LOCUS       NZ_CP136273             5596 bp    DNA     circular CON 19-OCT-2023
DEFINITION  Klebsiella pneumoniae strain L2425 plasmid unnamed3, complete
            sequence.
ACCESSION   NZ_CP136273
VERSION     NZ_CP136273.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37688352
            Assembly: GCF_032681225.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Fu,H.
  TITLE     K. pneumoniae L2425,complete genome
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Fu,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-OCT-2023) The First Affiliated Hospital of Zhejiang
            University School of Medicine, Zhejiang University, Qingchun Road,
            Hangzhou, Zhejiang 310000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/18/2023 17:34:57
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,087
            CDSs (total)                      :: 5,964
            Genes (coding)                    :: 5,771
            CDSs (with protein)               :: 5,771
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 193
            CDSs (without protein)            :: 193
            Pseudo Genes (ambiguous residues) :: 0 of 193
            Pseudo Genes (frameshifted)       :: 69 of 193
            Pseudo Genes (incomplete)         :: 129 of 193
            Pseudo Genes (internal stop)      :: 27 of 193
            Pseudo Genes (multiple problems)  :: 32 of 193
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP136273.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="L2425"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed3"
                     /geo_loc_name="China:Hangzhou"
                     /collection_date="2020-07-30"
     gene            complement(join(5474..5596,1..423))
                     /locus_tag="RX846_RS30385"
                     /old_locus_tag="RX846_30385"
     CDS             complement(join(5474..5596,1..423))
                     /locus_tag="RX846_RS30385"
                     /old_locus_tag="RX846_30385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            complement(449..775)
                     /locus_tag="RX846_RS30390"
                     /old_locus_tag="RX846_30390"
     CDS             complement(449..775)
                     /locus_tag="RX846_RS30390"
                     /old_locus_tag="RX846_30390"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            complement(1133..1291)
                     /locus_tag="RX846_RS30395"
                     /old_locus_tag="RX846_30395"
     CDS             complement(1133..1291)
                     /locus_tag="RX846_RS30395"
                     /old_locus_tag="RX846_30395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            1931..2107
                     /locus_tag="RX846_RS30400"
                     /old_locus_tag="RX846_30400"
     CDS             1931..2107
                     /locus_tag="RX846_RS30400"
                     /old_locus_tag="RX846_30400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            2097..2441
                     /locus_tag="RX846_RS30405"
                     /old_locus_tag="RX846_30405"
     CDS             2097..2441
                     /locus_tag="RX846_RS30405"
                     /old_locus_tag="RX846_30405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2650..>3084
                     /locus_tag="RX846_RS30410"
                     /old_locus_tag="RX846_30410"
                     /pseudo=""
     CDS             2650..>3084
                     /locus_tag="RX846_RS30410"
                     /old_locus_tag="RX846_30410"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3119..3628
                     /locus_tag="RX846_RS30415"
                     /old_locus_tag="RX846_30415"
     CDS             3119..3628
                     /locus_tag="RX846_RS30415"
                     /old_locus_tag="RX846_30415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            3635..3847
                     /locus_tag="RX846_RS30420"
                     /old_locus_tag="RX846_30420"
     CDS             3635..3847
                     /locus_tag="RX846_RS30420"
                     /old_locus_tag="RX846_30420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            4514..4738
                     /locus_tag="RX846_RS30425"
                     /old_locus_tag="RX846_30425"
     CDS             4514..4738
                     /locus_tag="RX846_RS30425"
                     /old_locus_tag="RX846_30425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            4722..4988
                     /locus_tag="RX846_RS30430"
                     /old_locus_tag="RX846_30430"
     CDS             4722..4988
                     /locus_tag="RX846_RS30430"
                     /old_locus_tag="RX846_30430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(5058..5408)
                     /locus_tag="RX846_RS30435"
                     /old_locus_tag="RX846_30435"
     CDS             complement(5058..5408)
                     /locus_tag="RX846_RS30435"
                     /old_locus_tag="RX846_30435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     Typing          1766..1845
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.361"
                     /coverage="97.0"
                     /evalue="1.1100000000000001e-21"
     Typing          complement(1183..1580)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          2432..3963
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gggagctact ctatataaca tataacctga gttaagggtt tcttttatac tttcttcaat
       61 gctcattata aatattagtg gattatcatt tttcttcccc catgggtttc taattaatcc
      121 ttcgatgtac tcaggtcctt tgcgtcctat tgcctgcgca atatttttta tttcatctgc
      181 gtgagggctt tgttgtacag ctttgtttat gcctgcttga gtgattttca gagactctac
      241 accgtgtatg ttgtatgaga taaaatgtaa taaattaaga tgcaatgcaa ttaatccatt
      301 tttgtttaca tctgagtatt tggataaaaa acgtaatgaa gtgttaactc tcccttgaag
      361 cctaacaatt gggagtgctg ccttgtatat ttccttctcc gttaaatacc gtttaaaaaa
      421 cataattctc cgcctccgta tagcgtgcct attttatttc gtcgtaaaac ttagctctga
      481 aatattcctt tagaaccatc atgtcattaa taattgaatc tatctcatcc attttttctt
      541 ggtattcttt taatggtgaa gggagcggta actcaatatt tgaaaagtga ttggcgagat
      601 agggatcaag gtttctcatc cattcaggct tttcgccaat gcgttcttca actgaaagtg
      661 aacctgataa tctctggtta ataagatcac ttagactcat tttggtttta ttcgaaagcc
      721 gttgtaagga ttgctttaat cctatatcta gcatgatatt tgttctttct ctcattaccc
      781 ccccttgcta ttgcgttttt tgttgcgcac aaagctacca ttgcgcatta agaaacgcaa
      841 taaaaaagga gggagggagt ggtttgtaca tgatgtgcag tgcgctgagg gcatcagtgg
      901 cgggtgtttt tccgtgggat ggatggagct ccggggataa cagaactgac gatcatttaa
      961 aggatcttct tgagatcctt tttttctgcg cgtaatctct tgcactgtaa acgaaaaaac
     1021 cgcctgggga ggcggtttga tcgaaggtta agtcagttgg ggaactgctt aaccgggtaa
     1081 ctgggtatac gagaccgcag ataccaaaac tgttctttca gtgtagccgc agttaggcac
     1141 ccacttcaag acctctcaat atctctctcg tatatatcca gttaccaatg gctgctgcca
     1201 gtggcgtttt tgcgtgtccg tccgggttgg actcaagatg atagttaccg gaaagggcgc
     1261 agcagtcggg ctgaacgggg ggttcgtgca tacagcccag cttggagcga acgacctaaa
     1321 ccgagccgag ataccagcag cgtgagctat gagaaagcgc cacgcttccc gaaagggaaa
     1381 aaggcggaac aggtatccgg taaacggcag ggtcggaaca ggagagcgca cgagggagcc
     1441 gccggagaga aatcaccggc atctttaagt cctgtcgggt ttcgtcctcc tctgacttga
     1501 gcgtccattt ctgtgatgct cgtcagggga ggcggagcct atggaaaaac ggcaacgccg
     1561 catccttttc tccggccctt tctgatttga gcgcctttgc tttatcccgt tatccgtgag
     1621 tcggtcacgc ccgccgcagc cgaacggcag gagcgcagct accgagtgag cgaggaggcg
     1681 tcatttcatc tgtccgtgca tggtattgcg gacgctcctg agccacatat cacacctgcc
     1741 acatgaggca ctttcatgaa tcgtgctctc atgccaacat agtaagccag tatacacagc
     1801 gtaaacgctg tgactggttc agggctgcgc cccgaaaccc gctaacacca ctgacgcgct
     1861 ggcgcttgtc caacgccgga gcgacccgga aaacttttcc gtgccgtccg gcgtttgcga
     1921 aggagggata atgcagaagc cgaagaagaa gccgctcgtc gtaaaaaaaa gatcctggca
     1981 tccggcagtg atggcaaatc tgatgcgtca ggcacaggag gaagattacc gccgggagca
     2041 gatggcagag cttgagcgtt cagcccggtc acattttgcg gatatcggag gagaggatgg
     2101 cggttaagcg tacccgtttt ttaggtatcc gggtgaccga tggggagtac cagcagctgc
     2161 tggagcgctg caatggaagg caactggcgg tgtggatgcg ggaaacctgt ctcgatacgc
     2221 gcccggcgcg gtcgttgcgg ctgccgtcca tcgatccggt tttgctgcgc cagcttgccg
     2281 ggatggggaa caacctcaac cagatcgccc ggaaaattaa cggcggccag tggtcaggcg
     2341 ctgaccgggt tcaggtggtg gccgcgctga tggccattga tgccgaactc gagcggctgc
     2401 ggcatgccgt gctggaaaag ggggcagacg atgatcgtta agtttcatcc gcgagggcga
     2461 ggcggcggtg ccgggccggt ggattatctg ctggggaaag accgccagcg cgacggggcc
     2521 atcgtcctgc agggaaagcc ggaggaggtc cgggagctta tcgacgcctc gccctacgcc
     2581 aaaaagtaca cctccggcgt cctgtctttt gctgaacagg atttaccgcc cggccagcgt
     2641 gaaaagctga tggcgagctt cgagcgggtt ctgatgcccg gactcgataa agaccagtac
     2701 agcgtgctgt gggttgaaca ccgggacaag gggcggctgg aactgaattt tctggtaccg
     2761 aacacggaac tgctgaccgg caggcgtctc cagccgtact acgaccgggc agaccgtccg
     2821 cgcatcgatg cgtggcagac cgtggtgaac gggcgactgg ggctgcacga cccgaacgca
     2881 ccggagaacc ggcgcgcgct ggtgacgcca tcttcgttac ccaaaacgaa gcaggaagcc
     2941 gcagaggcta ttacgcgggg cttactggcc ctcgcctcgt caggggagct taaaacgcgt
     3001 caggacgtca ctgaggcgct ggaaagcgca ggttttgagg tcgtgcgcac cacaaagagc
     3061 agcatcagca ttgccgaccc ggacggaggg cgaaacatcc gacttaaggg agccatctat
     3121 gaacagtctt ttaacgctgg cgaaggactt agagcagaaa tcgaaagcgc agcagcagag
     3181 taccggcgaa atgctgaaag ccgcattcag cgagcacgag aagtctgtca gagcggaact
     3241 gagcgaaagc gggaagagaa tcagcgccgc catccgcgcc cacgagcagg gtatgagcgc
     3301 ggccatgcag tcgaaccgcc tgagcgtgat gcgtatggtc gggcggacat ggctgaccat
     3361 cacgatggtg tcaggactgc tgttcgccag cctgagcggg gtgttgtggt atcaggggag
     3421 cttgatagcg tcaaatctgg ccgaaatcga ccggcagaac gcagcgctgt cgaagctgaa
     3481 cgcgaagacc tggggcgtga cgtacctgga agacagcaac ggacgttttc tggtgctgcc
     3541 gaaggggacg gccgtagaca ggacgcagag ctggacggtc gggaacgggc ggtcgaagca
     3601 gaacgcgctg agactggtga aggagtaagt gccgatgacc gagctggaaa aacagttgct
     3661 gagcgcatta gagcagctac agcaggacta ctcgaaaagg ctggacgagt gggagagcgc
     3721 cttcggggaa tggcggaaaa tgtcagggct tatgcaacgg gagaacgcgg cgctgagcga
     3781 gcgcgtgacg cgcttgagtc agcaggtgga acgcttgagc gggcaactgc agcgtttgag
     3841 ccggtagtgc agcgtcatga gatggccgtg gccgcagaac gggcgcacga gcaacggcag
     3901 cacgaaaagg aactgacgga agcgcgttcc cggcagcgaa gctatgacgg tccgtcgctg
     3961 ggctgaaatg caggaaaaca gggttctcat cgggcattta cgctgaaacc ggatgtcatt
     4021 cacctggctg catggctatg cagccaggta aaatttatct gtcgcgaacg gggcggttta
     4081 tcgggtggtt tgttgccggt tttacctgac tgccaccccg ttcgcggcga acccgtccgg
     4141 ggcggtgcgg gcaacgggtg ttatgtttaa tcagcgtgaa cggcagatac aaaaaagccc
     4201 cggagggctt ttaactactg acttattttt ttgaagattt gctgggtttc aatttgtcag
     4261 tatttcccgg cgttttttta ttgtcacgct ggcgtttatc aggttcccct gttgaatcgg
     4321 caattcgttt cggacccggc ttaagaccca taatcgttat cctttcaata gttaagagga
     4381 aagccctcaa gctagatata ttgaccgcgt tacggattat caagaggggc taacaagatt
     4441 attttttgtt cttttaggtt gattgtggta ctgttgatat acatgtatag caaatggtgt
     4501 tcgggaggtt tatatgcttg ctatccggtt atctgatgag attgagtccc gtctggactc
     4561 gctggcgaag caaaccggca gaacaaagac gttttatgcg cgggaagcaa tactggcgca
     4621 tctggaagac ctggaggatt attatctttc agcagaaact gctgcacgcg ttcgccgtgg
     4681 tgatgaagca gtgcattcgt ctgaagacgt gaggaagtca cttggtctgg acgattaact
     4741 attccgatcg ggcgctcaaa tcgttacgca agatggacaa acagaacgca cgacggattg
     4801 tggattttat gagtttacgc attgcagttg ctgccgatcc tcgccagtca gggaagccgc
     4861 tcaaaggtga gctgggcgag ttctggcgct atcgggtggg agattatcgc gttctgtgtg
     4921 agatccgaga tgacgagctt gttatccttg ccgccacgat tggacatcgc cgcgaagttt
     4981 acgactgaag cccgctatgc gggctttttt gatgccgaca acaggtatta tggtaaatac
     5041 tcgactaact acgtgttcta ctgtttgtcg gttttagtta accagcaggt tgtcttaacg
     5101 ataatgtagc cgttattttt aactaataag gtcatattgt taacgtcagg cataataaaa
     5161 gacattatgg cctcagagtt atcatcagaa gcagctctct gcattactac ttcgattccc
     5221 ttttctttga atctataagt atccagttgc ctattaaact tagaaagacc tacaaccata
     5281 cttagtttgt tatctttaac agtaagagat gcttcctgag taccattaca tcggtagtaa
     5341 gcggtgtaac cgtcagcaaa agatcgaccg gggaatataa atgctaggaa cgcaattaac
     5401 caatacatag ttgagaatct tggtattaca catttcacta tcctctcttt catttttcgt
     5461 tcaccatact ttgttatctg atttttaatt tttctatttt ataaaccaag tctctaccaa
     5521 aaaggcgaaa attatcctca aataataata attgtttaag aacatccatg aaagcagctg
     5581 cttcaccctc agttat
//
