LOCUS       NZ_CP136272            11970 bp    DNA     circular CON 19-OCT-2023
DEFINITION  Klebsiella pneumoniae strain L2425 plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP136272
VERSION     NZ_CP136272.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37688352
            Assembly: GCF_032681225.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11970)
  AUTHORS   Fu,H.
  TITLE     K. pneumoniae L2425,complete genome
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11970)
  AUTHORS   Fu,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-OCT-2023) The First Affiliated Hospital of Zhejiang
            University School of Medicine, Zhejiang University, Qingchun Road,
            Hangzhou, Zhejiang 310000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/18/2023 17:34:57
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,087
            CDSs (total)                      :: 5,964
            Genes (coding)                    :: 5,771
            CDSs (with protein)               :: 5,771
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 193
            CDSs (without protein)            :: 193
            Pseudo Genes (ambiguous residues) :: 0 of 193
            Pseudo Genes (frameshifted)       :: 69 of 193
            Pseudo Genes (incomplete)         :: 129 of 193
            Pseudo Genes (internal stop)      :: 27 of 193
            Pseudo Genes (multiple problems)  :: 32 of 193
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP136272.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11970
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="L2425"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed2"
                     /geo_loc_name="China:Hangzhou"
                     /collection_date="2020-07-30"
     gene            815..2779
                     /locus_tag="RX846_RS30315"
                     /old_locus_tag="RX846_30315"
     CDS             815..2779
                     /locus_tag="RX846_RS30315"
                     /old_locus_tag="RX846_30315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            2779..3510
                     /gene="mobC"
                     /locus_tag="RX846_RS30320"
                     /old_locus_tag="RX846_30320"
     CDS             2779..3510
                     /gene="mobC"
                     /locus_tag="RX846_RS30320"
                     /old_locus_tag="RX846_30320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            3517..4047
                     /locus_tag="RX846_RS30325"
                     /old_locus_tag="RX846_30325"
     CDS             3517..4047
                     /locus_tag="RX846_RS30325"
                     /old_locus_tag="RX846_30325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(4074..4253)
                     /locus_tag="RX846_RS30330"
                     /old_locus_tag="RX846_30330"
     CDS             complement(4074..4253)
                     /locus_tag="RX846_RS30330"
                     /old_locus_tag="RX846_30330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(4279..4707)
                     /locus_tag="RX846_RS30335"
                     /old_locus_tag="RX846_30335"
     CDS             complement(4279..4707)
                     /locus_tag="RX846_RS30335"
                     /old_locus_tag="RX846_30335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(4871..5185)
                     /locus_tag="RX846_RS30340"
                     /old_locus_tag="RX846_30340"
     CDS             complement(4871..5185)
                     /locus_tag="RX846_RS30340"
                     /old_locus_tag="RX846_30340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(5173..5451)
                     /locus_tag="RX846_RS30345"
                     /old_locus_tag="RX846_30345"
     CDS             complement(5173..5451)
                     /locus_tag="RX846_RS30345"
                     /old_locus_tag="RX846_30345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(5626..5991)
                     /locus_tag="RX846_RS30350"
                     /old_locus_tag="RX846_30350"
     CDS             complement(5626..5991)
                     /locus_tag="RX846_RS30350"
                     /old_locus_tag="RX846_30350"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(5988..6359)
                     /gene="tolA"
                     /locus_tag="RX846_RS30355"
                     /old_locus_tag="RX846_30355"
     CDS             complement(5988..6359)
                     /gene="tolA"
                     /locus_tag="RX846_RS30355"
                     /old_locus_tag="RX846_30355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(6633..6878)
                     /locus_tag="RX846_RS30360"
                     /old_locus_tag="RX846_30360"
     CDS             complement(6633..6878)
                     /locus_tag="RX846_RS30360"
                     /old_locus_tag="RX846_30360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            7523..9010
                     /gene="ltrA"
                     /locus_tag="RX846_RS30365"
                     /old_locus_tag="RX846_30365"
     CDS             7523..9010
                     /gene="ltrA"
                     /locus_tag="RX846_RS30365"
                     /old_locus_tag="RX846_30365"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     gene            9115..10800
                     /locus_tag="RX846_RS30370"
                     /old_locus_tag="RX846_30370"
     CDS             9115..10800
                     /locus_tag="RX846_RS30370"
                     /old_locus_tag="RX846_30370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            10810..11067
                     /locus_tag="RX846_RS30375"
                     /old_locus_tag="RX846_30375"
     CDS             10810..11067
                     /locus_tag="RX846_RS30375"
                     /old_locus_tag="RX846_30375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            11152..11301
                     /locus_tag="RX846_RS30380"
                     /old_locus_tag="RX846_30380"
     CDS             11152..11301
                     /locus_tag="RX846_RS30380"
                     /old_locus_tag="RX846_30380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     BGC             4115..11970
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(11405..11970)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.719"
                     /coverage="98.0"
                     /evalue="0.0"
     Typing          2780..3507
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.35e-140"
ORIGIN
        1 cgttttgcct cacatgttag tcccctgctt atccacggaa tctgtgggta actttgtatg
       61 tgtccgcagc gcccgccgca gtctcacgcc cggagcgtag cgaccgagtg agcgaggagg
      121 cgcaatttcc ccggcacctg caagacggtc accggacgcg ctgatgtggg atattgtccc
      181 ctgattaagt ctttctattc cctgacgatc aattatcagg ataggtgaac catgtcggtt
      241 tttcgctgaa cattttacgt taacctatct ctgttgcttt tttctccttg cggagaaatg
      301 caagtcatcc ccgctggggc gtggctgact tcaggggaaa agaccttgct tacgctggct
      361 ggacgccacc gacgcacggt cttttcccct ctttctggct ctgtccggct ggacgccggc
      421 cacgccagaa attcacccgg cccccgtcag gctcacgcct gaccacccac aaaaatccac
      481 ggtgctggac gcacctgacg aagcagcaaa gctgctcctg attttgcggg tggtcagccg
      541 ctcgctatgg cgaactctga cgggggccgg aacaccggaa aagaccgcac tggggtgccg
      601 ccttttcctc tcacacgctg gggcgtgctc gttgttcctc cggggctgcg ccttttccgc
      661 atggggcgga aagaggctgg cctccgcggt gaaagctgaa cgcaatcacc acggacgtga
      721 ccgccgccgc catgccttgg ggcatgtccg cggcttgtgc agcgggggct gcgcatcacg
      781 ccggtaaacc cgtcgtctga aggagagaga ttgcatgttt aatacggatt cgcttgcctg
      841 gcagtggcat atcccggagc tggcccggca gatttacccg tgggtcagct ggctgtaccg
      901 ggagtattac agtctggccg gtttttttct ggttatcggc ctgtttttcg gtctggctga
      961 actctgggcc ggccgaatgc gttttgtggg gctgagccat aaggcgcggc gggagtatgc
     1021 cgatcgctat aagcgcgata ataaccggac tgtgccattt tcggtgctgg cctcgctgga
     1081 ttcacccggg cggatggtca gggcacggcg acatgtctgg aactacgcgt actggctgac
     1141 cggcgctgcc ggtctggccc ttctgggggg gctggttgtc gccgtgttta tcgagagctg
     1201 gggagaaacc gtcctgcgcc cggacctcta tgcgaacgca gattattacc tgctgcagcc
     1261 ggctaacctg ttcgggatag cctggtgtgc cggagggctt gtcgccggcg tccttctggc
     1321 gcggctgctg gcgttcctgc tgcttgatgg tcattttgcg gccgctgacg aggcggtgaa
     1381 cgcgaaactg aatcaggaga gccggcgcag cagccagcgg accggagaaa tgacagacgt
     1441 ccgccacctg catttcgggg agccggtacc ggttaacgcg ctggccgatt tttccacgga
     1501 gcaggcccgg aaacagcagg cggtgtttct gggtaaggat gagcagggcc agccggtgct
     1561 ggtccctcgt gatacctggc gcaagaccaa tattcagatc ctcggcctgc ccgggagcgg
     1621 caagagcgcg atggccacca acgcgctcat tcgctgcgta cgcgatttcg gggacgctgt
     1681 ggtgtacttc gaccccaaag gggacgcgtg ggcgccgcac gtcttccggg cgcactgccc
     1741 ggacttcacc ctgctggatc tgcggccggg gaaaccggcg cagctgaatc tgttccgcga
     1801 tctcgaccag tacgccctga agaatctgct ggttgccggg tttaacctga gtgagaccgg
     1861 cgacgtggcg gaccactacc gcatcagcga gcagaaagcg gcgaagctga ttgcggagca
     1921 gttcccgcag ggggcaaaca ttcagcaggt gctggcggcg gcgtacgcgt tgccggaggc
     1981 cctgaaaaag gacgtgaagg ggctgatcac caagctggag aacgtggccg acctgagcgt
     2041 cctgcagacg gacagcggta tcgatgtggc ggggataatc aacggcggcg gctgcctgta
     2101 cgtcatcggc tcgatggacg atgaggcggt gatccgggtg cagaagatgc tgtttgcccg
     2161 ctgcgcgcaa atcatcattg cgcgggatga gttccggagg tggccgcacg cgagcatcat
     2221 gctcgatgag attaaatatc tgctgtcgaa gtacgtgctg aacgcgctgg ggacgctgcg
     2281 cagccgggac tgcaacctgc tgctcgcgca ccagtcgctg ggcgacttcg gtcagtgcgg
     2341 gcaggatctg cccgctgatt ttgtgaaaac cacggtactg gataacacgc cgatccgctg
     2401 gttctatcgc gcggccagcc aggagtccgc gcagtgggcg gcgggccaga ccggggagat
     2461 ccgggtggac gtggagcgcc gccgggccag ccgggaggcg gggaacgtgg agcacatcag
     2521 cggcgacagc ttcatccaga aggaggcgcg gccgctgttt gacgtgaaca ccctgcaaca
     2581 cctgcctgac gggttcgcgg tgatgaccgg gctgggcgtg gcgcggctgg cgttcagcag
     2641 cccgctgcgg gttgagcgcc gggaaatttc gctgaaaacc ttcccggtgc tggcgaaaac
     2701 ggacccgctg gcggagtacc tgcctccggc cgacggcggc ggaccaaaag cggatgatga
     2761 ttttgcgggg ctttactgat gacactggag cagtacaacg tcagccacgc gaagcggcag
     2821 gcgcggaacg cggagaaaac acggctgacc ctgcgctggc tgcgggaaga gctgtgttcc
     2881 acggcggagc tggttgcgcg acggctgggg attgcggcgg tacagccggt ttaccgcttc
     2941 ctggacagtc tggtggcgaa agggctgctg gtccgggcga aatacccggt tgacggccgt
     3001 caggtcagcg tgtgggggct gacgccgcac ggggtggcgt tctcgttcga cgaggacgag
     3061 ccgctgacgg acgttattcc gtttcagccg tcaagggtgt cggcggcgca gctgccgcac
     3121 cggctggccg tgcagtccct gcgcctctcc atggagggcc ggggggccac cggctggcgg
     3181 tacctgcacc gtatggccct gaaagggatg aaagtgccgg acgcgctggc ggagctggac
     3241 ggcagaacgg tggcatttga ggtagagcgc accgtcaaga gccgcaggcg gtatcaggaa
     3301 gtggtgtccg ggtatctgtt caaccgccgg gcgaacggca tcgatgaaat ctggtacatc
     3361 tgccctgacc ggccgacgct tgtccgggta cagcgggcga ttctggcggt ggatgaaatc
     3421 gtgaatccac agaccggcga ggcccgcaaa acggtggagc tggaccggga gaggctgttc
     3481 gcgtgcttta aatttatgac aacggagtga gggattatgg cgctgtacat ggtatttatt
     3541 gaggacgtgg aagacaaaga cggcgagcgc tgcattatgc caaccagcga gcatctggaa
     3601 gacatggtgg tgctcgcctg gcagaccggg gcgtatgttt tctgttttgt cgaccgggcc
     3661 cagcatctgg cgatgatgaa atccatcggg atgtatgacg agcggctcaa cagcgacgaa
     3721 aaagccacgt actttttcac cggtgaaaat ccgcaatggg aacgggacaa acccacagga
     3781 atgaagctgt gtgattacct ctatcgaaag gtggagcggt tcgccatcga gaacggtgtg
     3841 caggtggttg atgccgggaa gtgctcaccg gagcagatta acgcgatgat tgaggtggtc
     3901 agcgagcggc acggggaaag cgcggtgaac gagaagctga aagcggagct gatgcgggaa
     3961 tataacagcc gctattttaa cgtgccggtg cctgaactgc cggaggacaa acccgcccgc
     4021 cggtggtggc cgttcggcaa aaaataacgc cagcaccggc atggccggtg ctgttattct
     4081 tccccgagct tatcccgcat cacctgccag agtgtttccg catcctcgtg aagccgttcg
     4141 cacatgtcgg ccggggtatc catgtccagc tcgttcagct tttccagcaa cagcagcgac
     4201 tgatccttaa tgaatttagc catattgagg gcagtttttt cctgcttcgt catgttctcc
     4261 attcccttcg gtaagttctc agctaaacca gccggaccag aagcgttttt tcttctgtgt
     4321 cagttctgtt gtcaatctgt ccacttcggc ctggagtttc cggcgctcat catcgtgctg
     4381 gcggcgttct tcttcccggg atgttttatc ttccagcatc agggtgacag cctgccggag
     4441 atcggccagt tccttttgca ttgccagaag ctgttcctgt gggaaatttt tttgtgtatt
     4501 ttcacggtgt gaaatgccgg gaatttgttt ctcagggata ttcacatttc cgtatacacg
     4561 gatcagttct gacacatcca cgacagggtt gtttttcccg tcacgtgaca cggtaacctt
     4621 tccctgtttt acatgattgt atagcgttct tctggtaatg cccgcggctt gtgctgcctg
     4681 tgaaaggttg agcaatgttt tcgccactgt atacccctga ttttatcggg tgtgaaaggt
     4741 gtgtaatttc tcacggtaaa tgtatcgctg aattttaccc gtttttccgt tcaggatgtg
     4801 gttttctgac gcgctgcgct tgtcaaaccg gcgacggcca cgcaatgaat tgcgcccccg
     4861 tcatcggtta ttagaggctg tctgcccccg gatacccgga ttctttctga tgcctgacgg
     4921 tgacgatcac aatctggtcc atttcccggt cgtggcggta gagcatcaca tagccactgt
     4981 cgccaaaccc gatcaccagt tccttatatt ccagcggcag aaacggcacc gggcggccaa
     5041 tgtctggcaa tgttttcagt tgctggatag cccggacgat cacctcacct ccttttctgg
     5101 ctgccagccg atttttggtt ttgagaaagt cctgaaggcg ttgtaaatcg cgctgtgcca
     5161 gcgcggaaat cgttacctgt ggcatggcgg agcatcctgc tcgttctcag ttccccaggt
     5221 acttatccag gcttccgctt cttccgcagt aaggtgcaga cccgtttcct gatagtgctg
     5281 ccatgcggcc tgtccgtccc gcaggtactg gctgcgcttc tcttcccggt cgatgtattc
     5341 ggtgattgcc tccagcatta gcgcgtgcgc tgagcggtgc cggtcatcgg ccagcgtttt
     5401 cagccggtcc ttaagttctt catcgagcct gatggatgtt gctgatgaca taaaagcccc
     5461 tttgtagtca tatgtattac acatgactac tttaatccgt ggcggcgcta tttgccagaa
     5521 atacggcttt tgctgcccat ttttcagctt cgcttccact aatcagggct gctacaggct
     5581 cactcatcat ccaggactat tgcagcctgc gggttaaagg acctgttact ggcggcaacc
     5641 atcatcattg agcttttggg tggtgactgg tcgcggatcc tgtaagccgt catttttcag
     5701 actgaattca aaatttataa cctgtcgcat tgccgggtgc cctgcgggat atagccagtg
     5761 cctcattgcc gtcagcgttt cccgaaaaaa aaggtgacag ccggtaagcg acacgttgtc
     5821 cactcgtcca tcgtccagaa tatcaaacgt ggcggtcacc tgaccttcga tgcgttttgc
     5881 tgcggcctgt accgggtagg ttggctgcag tgtggttacg ggttttagtg gtatcgcatg
     5941 taccggagtg aggaacatgg ccgccagcac tgtcagagta aaagctttca tggtctgaaa
     6001 tccagagggg cgtttttgaa tatttgccag gtattttcat ccggtgccgg cggaattttg
     6061 gcgtgggtaa cggctgatat tgctgcttta cagagtttcg gatctcctcc ttcagcaatg
     6121 gcgctgttca gagtgccatc acgttgtatg gatatccgca cggtgcacat ttttcccgca
     6181 taggcctctg gttcaaacat ctgcatttca acagcggtct tgatggccgc agcgtagcta
     6241 ctgattttag atgacttatc cagagtataa attttcggcg gctgggtggt ggaatgctta
     6301 caggccgtca ggagtaaggg gctgcacaat gccagcagga taaagggtat taaacgcatc
     6361 gggttgatga cctgtcagta aaaaatgtta catgtatatt tgtcactctt tacggccacg
     6421 ttcatttaca taaacacggc ttatcgtatc gtttttacac caaacggccc gccatgcagt
     6481 tgcgtgcata ggcatgcagc gggttacaat ttaccgggcg cgtttccggc ggttttccgg
     6541 gtggtttgtt gcctgtttta tcccgtagcc gccggaaacg ccctcagacc gtctgagcgg
     6601 tgcgggcaat gcgacgttat ggtaaatacc ccttaccggc ggcgctgtgg ctgccaggtg
     6661 aaggtggttt ccgtagcctc gtatttctcc cgacgcattt cctgcgcccg cctggcttca
     6721 cgttcgtcct gaatggcacg aagcaccctt tcccgcacct gttgttttac cgcctttggc
     6781 agcgttttgt tccctttcag gcccatggcc agtgcctgag cgacaaaggc gtccgccagg
     6841 cgcttttctt cagcgggagt cagtttcagg tgcgtcatag tggcctccgg gcgtctcagg
     6901 tttcccgaac agtttaaccg caggtcctct cctcatgcca gttagctttc agatgtgtct
     6961 ataccatctg aaatgtaagt gtgatgaatg tcttaaataa taaaatttgt tgccatgaga
     7021 aattttcggg aaaagacgcg atctgcttac aggtggctcc gggcgctttt ttgtgcctga
     7081 agatgagaac taatctcata tattgacacc tgaaaacggg aggagtaagg taataattat
     7141 actgtgtata tgtacagtgt gcgccgggag accggttaga gatcaagggg tgaaagtccc
     7201 cgaccattga aggaccagca atccacaagg tccccgagtc atgcgttgca taccgcgagg
     7261 tatggggcga agcgttgaca ggggtgttga caggccagcc attgagccac gaaatgtata
     7321 ttaaattacc gggtgccgac gttgtactgt taacggaagg caacatcata gggtgcgaca
     7381 ctgcgagtgc cacatggacc cggcggggtc tgagaccctg gcatgtcaat acgatctcta
     7441 cgcgggaacc gggagatctc ccctctgacc atctgccagt gtcggagatg gcccgcaccg
     7501 ggaagacgag gagtcatagc cggtgatgta cggagaggag aagtcggact cgctcatagt
     7561 agcggcgaag caggcgaaca acccgaaagg agcggagtca gtggagcgaa ggagcggggc
     7621 caaggggaac gcggaacagc cacacatgcg ccggacacag agccgggaaa gcatgtcaca
     7681 gaggctgtca cgcgtgcggg aagctgcgaa gcagcggaag aaagaacggt ttacagcatt
     7741 gttccacctg ctgacagtcg aagcactgga agccgcattc ctctccctga gcaggaaagc
     7801 ggccgccgga gtggatggca tcaggtggat ggactacgcc ggaaacatga agaacaacat
     7861 aacagatctg caccggaggc tacatcaggg cagctacagg gcgcagcccg gcaggcgtca
     7921 ctacatccca aaagcggatg gaaaacaacg cccgctcggc atcgcctcgc tggaggacaa
     7981 gatcgtccag tatgcgctgg tgaaaatcct gaacgcagtc tatgaaaacg actttatggg
     8041 gttctcatac gggttcagac ccgggcgaag ccagcacgat gcactggacg cactggccac
     8101 agggctggta cgcactaacg taaactgggt actggatgcc gacatcagtc agttcttcga
     8161 cagggtgagc cacgaatggc tgatcaggtt cacagagcat cggatcggcg accggagggt
     8221 aatcaggctc atacgtaagt ggctcacagc cgggacgtcg gaggagggtc aatggcgagc
     8281 aacggaggaa ggcaccccac agggtgcggt catctcaccg ctgctggcaa acatatacct
     8341 ccactacgtc ttcgatctgt gggcgcatca gtggcgacgt cgctatgcca caggcaatgt
     8401 ggtaatggtc agatacgccg atgacatcgt catcgggttc gacaaacgat acgatgcccg
     8461 gcgcttccgt atagccatgc agcgcagact gagggagttc ggactcacgg ttcacccgga
     8521 gaaaacccgt ctgatggagt tcggccgctt cgctgccgaa aaccgtgcca tcaggggaaa
     8581 aggcaaacca gaaacgttca acttcctcgg gttcacgcac atcagcggga aagatcgcaa
     8641 cggcaggttc atgctgatac gaaagacccg ccgggatcgg atgacggcaa ctctgaaagc
     8701 catcaaagac ggtctgcgaa ggcgctggca ttactcaatc cccgaacagg gaaaatggct
     8761 caggagagtg gttcagggat acctgaacta tcactcggta ccgggcaact tccccaccat
     8821 gcagaagttc aggacacacg taacaaacct ctggcgccgg gcgctcaggc gcaggagcca
     8881 gaaggatgat acgacctgga cgaaagcaaa caaactggca gccgcatggc taccaagggt
     8941 tcgggttctt catccatggc ctgtggagcg gttcaccgcc agacacccga ggcaggagcc
     9001 cggtgcgtaa atcgcgcacg ccgggatctg tgcggggggt atccggtaac gggtatccct
     9061 accgcgacat gttggcttta atttattgtt ttaaatgtca aagaggaaaa cggtatgagt
     9121 ggtggagacg gtcgaggtcc gggtaattca ggtctgggac ataatggtgg tcaggccagt
     9181 gggaatgtga acggtacgtc tggtaaaggt ggcccttcat caggtggtgg tacggatcca
     9241 aacagcgggc cgggctgggg tacgacgcat acgccgaatg gagatattca taactataat
     9301 ccgggggagt ttggtaacgg cgggagtaaa cccggtggta atggtggtaa cagcggcaat
     9361 catagcggta gctctggtgg cggacagtct tcggccactg cgatggcctt cggtctgcct
     9421 gccctggcta ctccgggggc tgaaggactg gctttatcag tttccggcga tgcgttgtcg
     9481 gccgccgttg ctgatgtgct ggctgccctg aaggggccgt ttaagtttgg tctgtggggg
     9541 attgcgatct acggtgtgct gccttctgag attgcaaaag atgatccgaa aatgatgtca
     9601 aaaattatga cgtcattacc ggccgatgcg gtgacggaga ctccggcaag tactttacca
     9661 ctggaccagg cgacggttcg tgtcagacaa cgggttgtgg atgtggtgaa ggatgagcgg
     9721 cagcatattg cggttgtcgc aggtcggcca atgagtgtcc ctgtggtgga tgcgaaaccg
     9781 acaaaacgtc cgggggtatt cagtgtgtcg attccgggtc tcccgtctct gcaggtgagc
     9841 gtaccgaaag gtgttccgac agcgaaagcc ccgccaaaag gcattgttgc tgaaaaaggt
     9901 gattcacgtc cggctggttt tacagccggt ggtaactccc gtgaggccgt tattcgtttc
     9961 ccgaaagaga ccggacagaa gccggtttat gtgtcggtga cagatgttct taccccggca
    10021 caggtaaaac agcgtcagga ggaagaaaag cgtcgccagc aggcatggga cgccgctcat
    10081 ccggaagagg ggctgaaaag agactatgat aaagcgaaag ctgagctgga tgccgaagat
    10141 aaaaatattg cgaccttaaa cagccgcatt gcatcgacag agaaggcgct ccccggtgca
    10201 agggctgctg ttcaggaagc cgataaaaag gtaaaagagg cagaggcaaa taaagatgat
    10261 tttgtgactt ataacccgcc tcatgaatat ggctccgggt ggcaggatca ggttcgctat
    10321 cttgataagg atattcagaa tcagaatgag aaattaaaag cggctcaggc atctttaaac
    10381 gcaatgaatg aatccttatc cagagataag gctgcactta ccggggcgat ggagagccgg
    10441 aaacaaaagg agaaaaaagc gaaggatgca gaaaataagt taaatgagga aaagaacaag
    10501 cctcgcaagg ggactaaaga ctacggccat gattatcatc cagtacctaa aacagaagac
    10561 ataaagggac ttggagagtt aaagcgaggt gatcctaaaa cacctaaaca aggtggtggt
    10621 ggtaagcggg cccgatggta tggcgataaa aaacgtaaaa tttatgaatg ggattcccag
    10681 cacggtgagc ttgaagggta tcgtgccagt gatggcgaac acctaggcgc atttgatccc
    10741 aaaacgggta agcaggttaa agggccagat ccaaaacgga acattaaaaa atatctttga
    10801 gaggtaaata tggggcttaa attacgtttg acatggtttg ataagaaaac tgaagagttt
    10861 aaaggggaag agtattctaa agactttggt gacgatggtt ctgtcattga aagcttggga
    10921 atgcctttaa aggataatat taataatggt gagtttgatt tgaagaaaga atgggttcct
    10981 ttactacagc cattttttaa aaatcaaata aaacctgatg tggataattg ttatatagct
    11041 tttgattatc gggacggtaa ctggtaacaa aaaattatca aggatgattt gaaatactga
    11101 cttccggtgt tcagggatga atgccggagt ctttaaatac agggaaagta tatgaaaaag
    11161 gtaaagacta tttttctttt tatattgatt gttgctggct ttcttctggc cgcatgtcag
    11221 gcaaattata tccgggatgt tcagggcgga acggtggcac catcgtcctc ctctgaactg
    11281 acggggatcg cggttcagta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
    11341 gcgtaatctt ttgccctgta aacgaaaaaa ccacctgggg aggtggtttg atcgaaggtt
    11401 aagtcagttg gggaactgct taaccgggta actggctttc gcagagcaca gcaaccaaat
    11461 ctgtccttcc agtgtagccg gactttggcg cacacttcaa gagcaaccgc gtgtttagct
    11521 aaacaaatcc tctgcgaact cccagttacc aatggctgct gccagtggcg tttttgcgtg
    11581 tctttccggg ttggactcaa gatgacagtt accggataag gcgcagcagt cgggctgaac
    11641 ggggagttct tgcttacagc ccagcttgga gcgaacgacc tacaccgagc cgagatacca
    11701 gtgtgtgagc tatgagaaag cgccacactt cccgcaaggg agaacggcgg acaggtatcc
    11761 ggtaaacggc agggtcggaa caggagagcg caagagggag cgacctaccg gaaacggtgg
    11821 ggatctttaa gtcctgtcgg gtttcgcccg tactgtcaga ttcatggttg agcctcacgg
    11881 ctcccacaga tgcaccgaaa aagcgtctgt ttatgtgaac tctggcagga gggcggagcc
    11941 tatggaaaaa cgccaccggc gcggccctgc
//
