LOCUS       NZ_CP136175             8066 bp    DNA     circular CON 13-NOV-2023
DEFINITION  Acinetobacter baumannii strain ABO21-A049 plasmid pABO21-A049-2,
            complete sequence.
ACCESSION   NZ_CP136175
VERSION     NZ_CP136175.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37654779
            Assembly: GCF_033469325.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 8066)
  AUTHORS   Andre,A., Plantade,J., Durieux,I., Godeux,A.-S., Charpentier,X. and
            Laaberki,M.-H.
  TITLE     Acinetobacter baumannii in Animal ICU
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8066)
  AUTHORS   Andre,A., Plantade,J., Durieux,I., Godeux,A.-S., Charpentier,X. and
            Laaberki,M.-H.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-OCT-2023) CIRI, Inserm, 50 avenue Tony Garnier, Lyon
            69007, France
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: SEP-2022
            Assembly Method                   :: Unicycler v. 0.4.9
            Assembly Name                     :: CIRI-ABO21-A0049
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 829x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/12/2023 12:49:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,748
            CDSs (total)                      :: 3,654
            Genes (coding)                    :: 3,603
            CDSs (with protein)               :: 3,603
            Genes (RNA)                       :: 94
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 29 of 51
            Pseudo Genes (incomplete)         :: 21 of 51
            Pseudo Genes (internal stop)      :: 11 of 51
            Pseudo Genes (multiple problems)  :: 9 of 51
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP136175.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8066
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="ABO21-A049"
                     /isolation_source="hospital environment"
                     /db_xref="taxon:470"
                     /plasmid="pABO21-A049-2"
                     /geo_loc_name="France: Marcy l'etoile"
                     /lat_lon="45.79387130 N 4.70843765 E"
                     /collection_date="2021-11-08"
                     /collected_by="Maria-Halima Laaberki"
     gene            complement(join(7738..8066,1..1297))
                     /locus_tag="RWV42_RS18605"
                     /old_locus_tag="RWV42_18605"
     CDS             complement(join(7738..8066,1..1297))
                     /locus_tag="RWV42_RS18605"
                     /old_locus_tag="RWV42_18605"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_318591279.1"
                     /translation="MIVKFLGNKGGGSAGATIDYLLGKDRDRPGAVLLSGDPELTQRLA
                     DNLDFQNRYTVGVLSFEELNLEEHQKEAIMQSFEETLLAGLERDQYDITWVEHTDKGRL
                     ELNFVIPNVELSTGKRLQPYFDQADRPLVENWKQVTNFEHGLTDPHAPEKAQAIKALNS
                     QNLPSSVKEIKEQIGTAIAEQISNGNIQNRQDVVETLKGAGFEIARETDRSISIKNPDG
                     KRNIRLEGVIYENRQFDKQFAEEHRRAGQDYQRTSRERYDTALEKLQRLAESKQQGNRE
                     TFKVKPSNHPRPAAENQKRFAFEYSHGSSSGRVDYSVSNLSWRKLVVSEANPEKAAGIS
                     RDQSKSRTDTTRETDLAEGETAREPRQENFIHRSEEQQKSGRLREYRQPNGTEIGQIES
                     NVKTPFENPFNESNGGMELLRDSGSLFENLATSKEANQDLKQNNPNQTEQDNAKRLYES
                     LQRIIELFRQTIERAKRDKSKSERADRNLELSESSIENSERGISQSKSAIKQSEQQITT
                     AEQHIDVQIKLEQKSNKKDYGMER"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1287..1619)
                     /gene="mobC"
                     /locus_tag="RWV42_RS18610"
                     /old_locus_tag="RWV42_18610"
     CDS             complement(1287..1619)
                     /gene="mobC"
                     /locus_tag="RWV42_RS18610"
                     /old_locus_tag="RWV42_18610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003115802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_286418734.1"
                     /translation="MKEKTLIKRNKEIKVRLTEAEHHALLERMEGGELATWIRNTCLDE
                     KPNKRRNYKAADPQLLAALGRIGGNLNQIARQVNTVESDIEKLRAFAELAVIREQLQGI
                     LANYDR"
                     /name="mobC"
     gene            1909..2391
                     /locus_tag="RWV42_RS18615"
                     /old_locus_tag="RWV42_18615"
     CDS             1909..2391
                     /locus_tag="RWV42_RS18615"
                     /old_locus_tag="RWV42_18615"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_318591280.1"
                     /translation="MISLKQLINEQDEFNVYIDEKVEALRNHQNYLNKIIPEELVKTGL
                     TYTPVEKNPNKHIFTYSELKIERETKNPVDGKVPLSKAISDRLKIEIAICRKLNSDIRN
                     YTVLDRTKYKSEYKENGTYRYSVYPNEETDKELFNVLERIFEYEAKQPMEDKNLPY"
                     /name="hypothetical protein"
     gene            complement(2445..3326)
                     /locus_tag="RWV42_RS18620"
                     /old_locus_tag="RWV42_18620"
     CDS             complement(2445..3326)
                     /locus_tag="RWV42_RS18620"
                     /old_locus_tag="RWV42_18620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004917957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_318591281.1"
                     /translation="MKNVQLSDFFENLAHKPYCADELLYGLQIRPKKTAINMQYIQGNQ
                     PCMLHYFFFDLDRSEAVMAWYDENLPMPYWTAQTQKNGHAHICYKLEIPLCTSEFGSQK
                     AIAYASKVQAGLANKLGADVGYSHLITKNPFHPDWRTTFWTERAYTLDYLADFVDLPKK
                     LSKKQEVSGLGRNCTLFDTVRKWAYTAVRAHRGGIYNIWLEEVTKQCLSVNEAFLEALP
                     YSEIKATAKSIATYCWKKDAYCYQEFIDRQSRKGAIGGKKSKSGGRPSLGEPWKELGIS
                     RATYFRRKKESL"
                     /name="replication initiation protein"
     gene            3842..4723
                     /locus_tag="RWV42_RS18625"
                     /old_locus_tag="RWV42_18625"
     CDS             3842..4723
                     /locus_tag="RWV42_RS18625"
                     /old_locus_tag="RWV42_18625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015589626.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982-like element ISPsma1 family transposase"
                     /protein_id="WP_058199818.1"
                     /translation="MPIDEFIINIYLMVEQYYKIVVTKPLRSAGYAPKLSDPEVICMEM
                     VGEFLHLDQDKQIWQYFTQHWQDWFPAIGSYPNFAKHCANLWQVKQQIQDKVSQIEGRD
                     NIHFMDGFPIPVCHYGRAYRHKNYQDLAAFSYCAAKQERYYGFEGHLLVNLSGMIKGFT
                     VAPANVDERAVAPDITDHIHGLLGADKGYISPSLTSYYDAQGVDLQTPLRANMKEDRPK
                     PVVRRLMKARRIVETVIGQLSERFNIQRVRARDLWHLSHRLIRKILSHNLCFVLNKKLG
                     NPPLQFDLLISS"
                     /name="IS982-like element ISPsma1 family transposase"
     gene            4751..4957
                     /locus_tag="RWV42_RS18630"
                     /old_locus_tag="RWV42_18630"
     CDS             4751..4957
                     /locus_tag="RWV42_RS18630"
                     /old_locus_tag="RWV42_18630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family toxin"
                     /protein_id="WP_318591278.1"
                     /translation="MLTPAWGEVLNCLTNDIPLPAKYKDHALTGNHKGFRDCHIKPDLV
                     LIYRVQSDTVDFVRLGSHSEVFD"
                     /name="type II toxin-antitoxin system YafQ family toxin"
     gene            complement(5150..6046)
                     /locus_tag="RWV42_RS18635"
                     /old_locus_tag="RWV42_18635"
     CDS             complement(5150..6046)
                     /locus_tag="RWV42_RS18635"
                     /old_locus_tag="RWV42_18635"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015589625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RTG family carbenicillin-hydrolyzing class A
                     beta-lactamase CARB-16"
                     /protein_id="WP_015589625.1"
                     /translation="MDVRKHKASFFSVVITFLCLTLSLNANATDSVLEAVTNAETELGA
                     RIGLAVHDLETGKRWEHKSNERFPLSSTFKTLACANVLQRVDLGKERIDRVVRFSESNL
                     VTYSPVTEKHVGKKGMSLAELCQATLSTSDNSAANFILQAIGGPKALTKFLRSIGDDTT
                     RLDRWETELNEAVPGDKRDTTTPIAMVTTLEKLLIDETLSIKSRQQLESWLKGNEVGDA
                     LFRKGVPSDWIVADRTGAGGYGSRAITAVMWPPNRKPIVAALYITETDASFEERNAVIA
                     KIGEQIAKTVLMENSRN"
                     /name="RTG family carbenicillin-hydrolyzing class A
                     beta-lactamase CARB-16"
     gene            6198..7079
                     /locus_tag="RWV42_RS18640"
                     /old_locus_tag="RWV42_18640"
     CDS             6198..7079
                     /locus_tag="RWV42_RS18640"
                     /old_locus_tag="RWV42_18640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015589626.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982-like element ISPsma1 family transposase"
                     /protein_id="WP_058199818.1"
                     /translation="MPIDEFIINIYLMVEQYYKIVVTKPLRSAGYAPKLSDPEVICMEM
                     VGEFLHLDQDKQIWQYFTQHWQDWFPAIGSYPNFAKHCANLWQVKQQIQDKVSQIEGRD
                     NIHFMDGFPIPVCHYGRAYRHKNYQDLAAFSYCAAKQERYYGFEGHLLVNLSGMIKGFT
                     VAPANVDERAVAPDITDHIHGLLGADKGYISPSLTSYYDAQGVDLQTPLRANMKEDRPK
                     PVVRRLMKARRIVETVIGQLSERFNIQRVRARDLWHLSHRLIRKILSHNLCFVLNKKLG
                     NPPLQFDLLISS"
                     /name="IS982-like element ISPsma1 family transposase"
     gene            complement(7105..7386)
                     /locus_tag="RWV42_RS18645"
                     /old_locus_tag="RWV42_18645"
     CDS             complement(7105..7386)
                     /locus_tag="RWV42_RS18645"
                     /old_locus_tag="RWV42_18645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family toxin"
                     /protein_id="WP_317457340.1"
                     /translation="MRKILAESQFKRDIKKQFLELITPAWAEVLHCLCNDLDLPEKYCD
                     HGLTGEWDGFRDCHVKPDLVLIYAIKNDYLQLVRIGSHSELFGKRRNK"
                     /name="type II toxin-antitoxin system YafQ family toxin"
     gene            complement(7379..7669)
                     /locus_tag="RWV42_RS18650"
                     /old_locus_tag="RWV42_18650"
     CDS             complement(7379..7669)
                     /locus_tag="RWV42_RS18650"
                     /old_locus_tag="RWV42_18650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282256.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="WP_252409991.1"
                     /translation="MATTNFNLRLEQDLRDKAFPVFERYGLSASQAFKLFLTQVAETNK
                     IPLSFDYAEDDNVPNSVTRKALLEAKNRTDFSEAYATPEEFMKAMQELANA"
                     /name="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
     ARG             complement(5154..6046)
                     /name="blaCARB-16"
                     /gene="blaCARB-16"
                     /gene_name="RTG family carbenicillin-hydrolyzing class A
                     beta-lactamase CARB-16"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
ORIGIN
        1 ttgaggtggc taagttttca aataaacttc cgctatctct caaaagttcc attccaccat
       61 ttgattcgtt aaatggattt tcaaaaggag ttttgacgtt agattctatt tgtccaattt
      121 cagtaccatt tggctgtctg tactctcgta agcgtccgct tttttgctgt tcctcgctac
      181 gatgtataaa gttttcttgt cttggctctc ttgcagtctc accttccgca agatctgttt
      241 ctctagtggt gtctgttcta cttttagatt gatctcggct aattcctgct gctttttctg
      301 gatttgcttc gctaaccacc aatttacgcc aagaaagatt agaaacactg taatcaacac
      361 gcccagaact gctcccgtga gaatattcaa acgcaaagcg tttctgattt tcggctgctg
      421 gtcgtggatg gtttgaaggt ttgaccttaa aagtttctcg gtttccttgc tgcttgctct
      481 cagcaaggcg ttgtaacttt tcaagtgctg tgtcgtaacg ctctcggctt gttctttgat
      541 aatcctgtcc tgctcggcgg tgttcttctg caaattgctt atcaaattgt cgattttcat
      601 aaattacgcc ttctaaacga atattccgct tgccgtcagg attcttaata ctgattgatc
      661 tatctgtttc tctagcaatc tcaaagcctg cacccttcag ggtttcaact acatcttgcc
      721 tattttggat attgccattg ctaatttgct cagcaattgc ggtcccgatt tgttctttaa
      781 tttctttgac gcttgaaggt aggttttggc tattcaaagc tttaattgct tgagcttttt
      841 ctggtgcatg aggatctgtt aagccatgct caaaattggt gacttgcttc cagttttcta
      901 caagtgggcg gtcagcttgg tcaaaataag gctgtaatct ttttcctgta cttaattcca
      961 catttggaat cacgaaatta agctctaaac gccctttatc agtgtgttct acccaagtaa
     1021 tgtcgtattg atcgcgctct aagcctgcaa gcaatgtttc ttcaaagctc tgcatgatgg
     1081 cttctttttg gtgttcttcg aggtttaatt cctcaaatga aagcactcca accgtatagc
     1141 gattttggaa gtccaggtta tctgctaatc gttgggttaa ttcaggatct ccactcaata
     1201 aaactgcacc tggtctatcc ctatctttgc caagtaggta gtcaattgtt gcgcctgcac
     1261 taccacctcc cttatttccc aaaaatttaa cgatcatagt tggctaagat tccttgtagt
     1321 tgttctctaa tgactgccaa ctctgcaaag gctctaagtt tttctatatc agattccaca
     1381 gtatttactt gtctcgcaat ctgattgagg ttgccaccaa tacgccctaa agctgctaaa
     1441 agttgaggat ctgccgcttt ataatttctt cttttatttg gcttctcatc tagacaagta
     1501 tttcgaatcc aagttgcaag ctctccacct tccatacgtt ccagtaaggc gtgatgttca
     1561 gcttctgtta aacggacttt gatttcctta ttacgtttta taagcgtttt ttctttcata
     1621 acaactcatc aaatacttgg tgcttttggg ttttggggtc ggcttttttc cgtaagaatt
     1681 ttggctatgt ttcatggcca taaatcttgc ggaaataaag tcgaaaagag ggtcaaggga
     1741 ttcttccctt gcaaggcaca aaaggtaatc tagcgaaaac tttagttttt gtctagagta
     1801 ccctttgtgt gtccttgccc tattaaaaaa aataataata attagcctaa tatctggtta
     1861 ttgttaataa gtgctagaat tgtattcaag aagatttgga ttgaaaaaat gattagctta
     1921 aagcagctta taaatgagca agacgagttc aatgtttata tagatgaaaa ggtagaagcc
     1981 ctaagaaatc atcagaatta tttaaataag attattccag aggaattagt taaaactggt
     2041 cttacatata ccccagtaga aaagaatcct aacaagcaca tttttactta ttctgaatta
     2101 aaaatagaaa gagaaactaa gaatcctgtg gatggaaaag ttcctttatc aaaagctata
     2161 tctgatcggt tgaagataga gattgctata tgccgtaaat tgaactctga cattagaaat
     2221 tatacagttt tggatagaac aaaatataag agtgaatata aagagaatgg aacttatcgt
     2281 tacagtgttt accctaatga agaaacagat aaagaattat ttaatgtttt agaaagaatc
     2341 tttgaatatg aagcaaagca acctatggaa gataaaaact taccttattg agggcaaaaa
     2401 agctgatatc tgatttaggc ttgtttagtc tcacaaaaga gagtttataa actctctttt
     2461 tttcttcgga aatatgtagc tcgactaatt cctaattctt tccaaggctc tcctaatgaa
     2521 ggtctaccac ctgatttaga cttcttacca cctatagcac ctttacggct ttgacgatca
     2581 ataaactctt ggtagcaata ggcatctttt ttccaacaat acgtagcgat acttttagcg
     2641 gtagctttga tttctgaata tggtaatgct tctaaaaacg cctcatttac gcttaaacac
     2701 tgtttagtga cttcctctag ccaaatatta taaataccac ctctatgcgc tctaacagcc
     2761 gtatatgccc atttacgcac tgtatcgaac agtgtgcagt tacgtccaag tcctgaaacc
     2821 tcttgtttct tacttaattt cttaggtaaa tcgacaaaat cagctaaata atcgagtgta
     2881 taagctcttt ctgtccaaaa agttgtgcgc caatcaggat gaaaaggatt tttagtaatg
     2941 agatggctat atccaacgtc tgcaccaagt ttattggcta gtcctgcttg tacctttgaa
     3001 gcataggcta ttgctttttg actgccaaac tcactggtac acagcggaat ttctaattta
     3061 tagcaaatat gagcatgacc atttttttgt gtttgggctg tccaataggg cattggtaaa
     3121 ttttcgtcat accacgccat gacagcttct gaacggtcta ggtcaaaaaa gaagtaatga
     3181 agcatacaag gctgattgcc ctgtatgtat tgcatgttga ttgccgtttt tttcggtctt
     3241 atttgtaagc catacaataa ttcatctgca cagtatggtt tatgagcaag attttcaaaa
     3301 aaatcgctta attgtacgtt tttcaccttt tgcaacgtcc tgtatgttgc aaataatcaa
     3361 aacatcggta aaatacaaat atcgattttg tgtttactga tgctttaagt atttgttaaa
     3421 caaagagaaa aaagtgagcc tgccagcttg cttttttttc gcctatagaa aatattacac
     3481 tatttcccag ttaaacaact aattgatttt tattaattta tgagttgcag tcactatcag
     3541 taccatcaat tttaactttc tcaccattcc tgataacagt tttttctatc aacaccattt
     3601 tatgggtaat agggtcttcc tcacataaaa tatgtgtacc tccctcatcc caagaatgag
     3661 tgccataaat catatagata taaaatatga atatagaaat gatagggaca aaggttaata
     3721 ctaaaatcca aataaatatt tttctatatg acaccccact ctcaacttcg aaagtgattg
     3781 aaaaaagaag agcacctcac taatctattg ttttcgacca agaatacaat ggagttgtga
     3841 gatgcccata gacgaattta tcatcaatat ctatttaatg gtagagcaat attacaaaat
     3901 agtcgtcacc aaacccttgc gaagtgcagg ttacgcgcca aagttgagtg atcctgaggt
     3961 tatttgcatg gagatggtcg gtgaattttt acaccttgat caagacaaac aaatttggca
     4021 atactttacc cagcattggc aagactggtt tccagccatc ggctcatacc ctaacttcgc
     4081 aaagcactgc gccaatctgt ggcaagtcaa acagcagata caagataaag tcagtcaaat
     4141 tgaaggccgt gacaacattc attttatgga tggctttccg atacccgtct gtcattatgg
     4201 acgcgcttat cggcataaaa actatcaaga cttagcagct tttagttact gcgccgctaa
     4261 gcaagagaga tactatggct ttgagggaca tttgcttgtt aatttatctg gcatgattaa
     4321 aggctttacc gttgcccctg ccaatgttga tgaaagagcg gttgcgcctg atattactga
     4381 ccatatccat ggtttgctcg gcgctgataa agggtatatc agcccgagtc tgacatcgta
     4441 ctacgacgct caaggagtag atttacaaac gccactcaga gccaacatga aagaggatag
     4501 acccaaacct gtggtaagac ggctaatgaa agcccgccgt atcgttgaaa cagtcattgg
     4561 tcagctatca gaacgattta atatacaaag agtgcgagca agagatttat ggcatttgtc
     4621 tcatcgattg attcgtaaga ttctttcaca caatctgtgc tttgtactca acaaaaaact
     4681 tggtaatccg cctcttcagt ttgatttgct tatttcaagt tgagagtggg gtaaaaagaa
     4741 ttatctaagc ttgctcactc ctgcatgggg cgaggtgctt aattgtttga cgaacgatat
     4801 accgctaccg gctaaataca aagaccatgc gttgaccggc aatcataaag ggtttaggga
     4861 ttgccatatt aagcctgatt tggtgcttat ctaccgtgtt cagagcgata cggtagattt
     4921 tgttaggctg ggtagccatt cagaggtgtt tgattagcct gttttaagtg gttccacaat
     4981 gacagtctgt tttgagtagt ctgttaatag gtttttgggg tgaaacagct ggttccgctg
     5041 gggtatacct aaatagaacc agtaagaact gatttctgac atccaacgtc ataaactctg
     5101 ttgtgggaac atgatcggtg cgctatacag gcatcgaact ataaataaat cagttacggc
     5161 tattctccat taatactgtc ttcgctattt gctcaccaat ttttgcaatg acagcatttc
     5221 tttcttcaaa cgaggcgtct gtctctgtaa tgtatagagc ggctacgata ggcttgcgat
     5281 ttggaggcca catcaccgca gtaatagcac gcgacccata accaccagcg cctgttctat
     5341 ctgctactat ccagtcactt ggaacgcctt tacgaaacaa tgcatcgcca acctcattac
     5401 ctttaagcca agattctagt tgttgacgag atttgataga tagtgtttcg tcaattagta
     5461 acttttcaag tgtcgttacc attgcaattg gtgttgtcgt gtctcgctta tctccaggca
     5521 ccgcttcgtt aagttctgtt tcccagcgat caaggcgcgt agtatcgtcg ccaatggaac
     5581 gcaaaaattt cgttagagcc ttaggtccac caatcgcttg tagaataaaa ttggcagctg
     5641 aattatcact ggttgataat gtggcctgac acagctctgc gagcgacatc ccttttttac
     5701 ccacatgttt ttctgttaca ggtgagtatg taacgagatt gctttcagag aatctcacaa
     5761 ctctatcaat tctttcttta cctagatcaa ctctttgaag aacgtttgca caggcaagtg
     5821 ttttaaaggt gctacttaga ggaaaacgtt cattagattt atgttcccaa cgttttcccg
     5881 tttccaaatc atgcacagct agaccaattc tagcgcctaa ttcagtttca gcattggtaa
     5941 ccgcttcaag tactgagtct gttgcattag catttaatga tagcgtgaga cataaaaaag
     6001 taattactac gctaaaaaaa ctagccttgt gtttacgtac gtccatgatg agttaacttt
     6061 ccttttggat caaattttta gattatataa tatgtaatta acaagtatac cccactctca
     6121 acttcgaaag tgattgaaaa aagaagagca cctcactaat ctattgtttt cgaccaagaa
     6181 tacaatggag ttgtgagatg cccatagacg aatttatcat caatatctat ttaatggtag
     6241 agcaatatta caaaatagtc gtcaccaaac ccttgcgaag tgcaggttac gcgccaaagt
     6301 tgagtgatcc tgaggttatt tgcatggaga tggtcggtga atttttacac cttgatcaag
     6361 acaaacaaat ttggcaatac tttacccagc attggcaaga ctggtttcca gccatcggct
     6421 cataccctaa cttcgcaaag cactgcgcca atctgtggca agtcaaacag cagatacaag
     6481 ataaagtcag tcaaattgaa ggccgtgaca acattcattt tatggatggc tttccgatac
     6541 ccgtctgtca ttatggacgc gcttatcggc ataaaaacta tcaagactta gcagctttta
     6601 gttactgcgc cgctaagcaa gagagatact atggctttga gggacatttg cttgttaatt
     6661 tatctggcat gattaaaggc tttaccgttg cccctgccaa tgttgatgaa agagcggttg
     6721 cgcctgatat tactgaccat atccatggtt tgctcggcgc tgataaaggg tatatcagcc
     6781 cgagtctgac atcgtactac gacgctcaag gagtagattt acaaacgcca ctcagagcca
     6841 acatgaaaga ggatagaccc aaacctgtgg taagacggct aatgaaagcc cgccgtatcg
     6901 ttgaaacagt cattggtcag ctatcagaac gatttaatat acaaagagtg cgagcaagag
     6961 atttatggca tttgtctcat cgattgattc gtaagattct ttcacacaat ctgtgctttg
     7021 tactcaacaa aaaacttggt aatccgcctc ttcagtttga tttgcttatt tcaagttgag
     7081 agtggggtat atgacataac agttctattt atttctacgt tttccaaata attcagaatg
     7141 actaccaatt cgtaccaatt gcaaataatc attttttatc gcatagatca gcactagatc
     7201 aggctttaca tgacaatctc taaaaccgtc ccattcacca gtaagaccgt gatcacaata
     7261 cttttcaggc aaatctaaat cattgcaaag acaatgtaag acttctgccc atgcaggtgt
     7321 gattagctct aaaaattgct ttttaatatc tcgcttaaat tgactttcag ctaatatttt
     7381 acgcattggc taactcttgc attgctttca taaattcttc gggtgtcgca taagcttctg
     7441 agaaatcagt tctattttta gcttcaagca atgcctttct agtaacacta ttcggtacat
     7501 tatcatcttc tgcataatca aaagacaaag gaattttatt ggtttcagca acttgagtta
     7561 aaaataattt gaaagcttgt gatgcactca atccataacg ctcaaatact ggaaatgctt
     7621 tatcacgtaa atcttgttca agtcgaagat tgaaatttgt tgtagccata tcatcaccta
     7681 tattttacat aatgcgtact atagtaaatt aaatgtacgc caaaaaataa tatttttcta
     7741 cctttccata ccgtaatctt ttttattcga tttctgctct aactttattt ggacatcaat
     7801 gtgctgttca gctgtcgtaa tctgctgttc agattgttta attgctgatt ttgattggct
     7861 aattcctcgt tctgaattct caatacttga ttctgaaagc tcaaggtttc gatctgctcg
     7921 ctctgacttt gatttatctc ttttagctcg ctcaattgtt tgtctaaaga gttcaataat
     7981 tcgttgtagc gattcataaa gtcgtttggc attgtcttgt tccgtttgat taggattatt
     8041 ctgttttaag tcctgatttg cttctt
//
