LOCUS       NZ_CP135725             2101 bp    DNA     circular CON 07-OCT-2023
DEFINITION  Escherichia coli strain TUM1223 plasmid pMTY1223_Col1, complete
            sequence.
ACCESSION   NZ_CP135725
VERSION     NZ_CP135725.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN35768940
            Assembly: GCF_032386315.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Komori,K., Aoki,K., Ishii,Y. and Tateda,K.
  TITLE     Analysis of the Plasmid-Driven Acquisition of blaCTX-M-14 in
            Escherichia coli ST131 Subclone H30
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Komori,K., Aoki,K., Ishii,Y. and Tateda,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2023) Department of Microbiology and Infectious
            Diseases, Toho University, 5-21-16, Omori-Nishi, Ota-Ku, Tokyo
            143-8540, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 13-SEP-2021
            Assembly Method                   :: SPAdes v. v3.15.5
            Assembly Name                     :: Toho-u_TUM1223_Comp_v1.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: missing
            Genome Coverage                   :: 141x
            Sequencing Technology             :: Complete Genomics
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/07/2023 15:05:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,045
            CDSs (total)                      :: 4,927
            Genes (coding)                    :: 4,738
            CDSs (with protein)               :: 4,738
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 189
            CDSs (without protein)            :: 189
            Pseudo Genes (ambiguous residues) :: 0 of 189
            Pseudo Genes (frameshifted)       :: 68 of 189
            Pseudo Genes (incomplete)         :: 124 of 189
            Pseudo Genes (internal stop)      :: 39 of 189
            Pseudo Genes (multiple problems)  :: 40 of 189
            Pseudo Genes (short protein)      :: 4 of 189
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP135725.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="TUM1223"
                     /db_xref="taxon:562"
                     /plasmid="pMTY1223_Col1"
                     /geo_loc_name="Japan"
                     /collection_date="1997"
                     /collected_by="Toho University School of Medicine"
     gene            complement(49..315)
                     /locus_tag="RS156_RS25215"
                     /old_locus_tag="RS156_25215"
     CDS             complement(49..315)
                     /locus_tag="RS156_RS25215"
                     /old_locus_tag="RS156_25215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024536.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     gene            complement(319..1284)
                     /locus_tag="RS156_RS25220"
                     /old_locus_tag="RS156_25220"
     CDS             complement(319..1284)
                     /locus_tag="RS156_RS25220"
                     /old_locus_tag="RS156_25220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001287824.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     Typing          complement(660..891)
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 gcaatctcgt acctatcacc tcagcctgcc gctggctctt ttccagaact aagaatgcgt
       61 tcttaaaaat ccaaacttga taccgtttaa atctattttc acaaaaagag tttttaccct
      121 ctcgtttgtt ttctgaatat ataaaattaa ttcatcctca aaaccttcgt gcggatgaaa
      181 accaaatgtg ccaaaactta atccatcaat aagcaaaata aggtttttaa acctgtcagg
      241 aatcaccgct tcacttctta aaaaaaataa accaatagtc ttatttccat tcaggtattc
      301 aatattaata ttcatacgtt aatccgattt atccttctca ggggcgcgtt tgtaacgccg
      361 tttacctgaa tcccagacaa acgccgtccg cttcccgtca tcagtgccat cccctacatc
      421 gtcggcaatg acaagatcct cattggtttc tcgatccaac tggaggacgt tttttagcgc
      481 cccaccggtc gagataaaac ggcgcttgtg aagctgccgc gtcagctcaa gaaaccactc
      541 aggatcgttt gccatatctt ccggtttaac ggagtatttc agcgtttcag caaccgcgct
      601 ttgcagttgc tcggcaacgt tggccacaac ctcacctgtc ttagttttta ctgcccgaat
      661 atcgatattc ggctcatagt tcacccgcaa gcaatcgcgc cagagttcta cccaacgttc
      721 gtgcttaacg tagttcttcc ctttaaacca agaaggttgc accatcagca gacagtgaaa
      781 atgcggatgt gcgctgccat ccttacctcg cgtcacctcc gtagccctga tccacccctg
      841 aacaggtgat agctcctttc gcttttccat tcgcttaaac gccgcattca ttgctgtaag
      901 gactgttcca agttcaccta tctcgcagtt cctgacagtt aacgtcagaa acaaccatcg
      961 ggaagacggg taatccacaa cgattttcgg tagagcctga taaaaacggg cttgccacat
     1021 gagggttctt ctccactggc agacagggca atgacggaca cggcaaaatt gcgcactacg
     1081 aagccgtagg cgcgtttctc cggtttccat gatggtagac caaccaaaac gtaacaaacc
     1141 gctacaggag gccatacgga gtgcatagcg ctcgaattca gcagcagtct ggtagatacc
     1201 cccgacagaa tcggtaagcg tgcgctgggt atcccaaact gcatcacggg ggctgtagtc
     1261 cgaaaggaat ttatcttctg acattaagcc tgctccgatc tgggcaggct gtgatttgat
     1321 gtggtatatt catcacgtac ctgtgcattg cgagccagta catgtcacca gagtttgccg
     1381 ctgaggtgac acattcgaaa tgttggggag tgttgacgca ctccccaacg ccgattaaaa
     1441 acgcctcaat tctgctccat tttctttgac ctgtcaaaac ccactcaaag cctcgtcatt
     1501 actgggctaa accactattt catatccgcc atttatccct taagtataaa tacaaggatc
     1561 acgcgcgaga attgcgcaaa aaataaccac aattttaacc ccccaaaaca agcacccccg
     1621 aacggcttac cccaaccttt tccacacacc cgatcaccaa aacagggaat aaaaacaaaa
     1681 ccctgtctta caggtggatg gcctcacttc gtgaaaccat ccaccccgca taagtttttt
     1741 aatcggtggt gtcgtcaatc actaaatcgg aaccccaaag gggaccccca cgatttagca
     1801 tttgacgaga ttaactctcg gagatggcat catgctttta acggacttta aaaaacggtg
     1861 aacggatgcg gattttaatc cgtgtctgtg aggcgttgag caacgagcaa agcgaggcgg
     1921 tagaaagaga gccagcggca ggctcaggac gataatccgg ggaaaatgta ccactcgcag
     1981 agtgttaaca tttgacatcg gattgtctat cctgcattgg tgtcgagttg gcaaggctga
     2041 ggataatgct gaggataatg ctgaggataa tgcaggatag acagagcact agagattgca
     2101 t
//
