LOCUS       NZ_CP135718             2097 bp    DNA     circular CON 07-OCT-2023
DEFINITION  Escherichia coli strain TUM1586 plasmid pMTY1586_Col, complete
            sequence.
ACCESSION   NZ_CP135718
VERSION     NZ_CP135718.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN35768946
            Assembly: GCF_032375165.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Komori,K., Aoki,K., Ishii,Y. and Tateda,K.
  TITLE     Analysis of the Plasmid-Driven Acquisition of blaCTX-M-14 in
            Escherichia coli ST131 Subclone H30
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Komori,K., Aoki,K., Ishii,Y. and Tateda,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2023) Department of Microbiology and Infectious
            Diseases, Toho University, 5-21-16, Omori-Nishi, Ota-Ku, Tokyo
            143-8540, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 13-SEP-2021
            Assembly Method                   :: SPAdes v. v3.15.5
            Assembly Name                     :: Toho-u_TUM1586_Comp_v1.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: missing
            Genome Coverage                   :: 90x
            Sequencing Technology             :: Complete Genomics
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/07/2023 14:16:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,396
            CDSs (total)                      :: 5,269
            Genes (coding)                    :: 5,070
            CDSs (with protein)               :: 5,070
            Genes (RNA)                       :: 127
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 99
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 68 of 199
            Pseudo Genes (incomplete)         :: 148 of 199
            Pseudo Genes (internal stop)      :: 29 of 199
            Pseudo Genes (multiple problems)  :: 43 of 199
            Pseudo Genes (short protein)      :: 4 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP135718.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="TUM1586"
                     /db_xref="taxon:562"
                     /plasmid="pMTY1586_Col"
                     /geo_loc_name="Japan"
                     /collection_date="2002"
                     /collected_by="Toho University School of Medicine"
     gene            383..1372
                     /locus_tag="RS158_RS26980"
                     /old_locus_tag="RS158_26980"
     CDS             383..1372
                     /locus_tag="RS158_RS26980"
                     /old_locus_tag="RS158_26980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_024136759.1"
                     /translation="MKNPDLQNLTDYSPSDAPWDAHRSVSDDVGGIYLLAAEYERYGAR
                     MALCGGLLRFGWSTLKETGETRLRLREAHFCRVRHCPVCQWRRSLMWQARFYQSLPRIV
                     ADYPDARWMFLTLTVRNCAIGELGEMLNRMNAAFQRMKVRKEFRPVQGWIRTTEVTRGS
                     DGSAHPHFHTLMMVPPGMLNGKSYVRHERWVELWRECLRVDYDPNVDVRAVKPRKPKGG
                     ESLACATAELVRGAVAETLKYSTKPADMVADPEWFLELTRQTHKRRFVATGGALKDVLK
                     LDQETDADMVIGDDISEGDDDGSRIAFEWKTEVRKYRRSPTKDKTESG"
                     /name="protein rep"
     Typing          1375..1456
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="93.976"
                     /coverage="90.0"
                     /evalue="3.4000000000000004e-27"
     Typing          complement(1099..1290)
                     /name="ColpVC"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.964"
                     /coverage="100.0"
                     /evalue="6.14e-93"
ORIGIN
        1 gttcacaaaa tcggggtgaa agtctgtgga tgagatgggt gtaagtgggt ggggttgggt
       61 ggttttgggt tactgaaaat cccgctcaaa attcaagcaa atctcgcgcg cgatccttgt
      121 atttatactt aagggataac tctaggattc aaattcgcct ccagcccttg tgccgcaagg
      181 cctgaggggg gatttgtcgg aaaatggttt tggagttatt ctgtcgggag tggtcgcatt
      241 aaaagcgacc cccgcaacga gggcaatcgt tcgggggtgt gaccacctgt aggaattcga
      301 attggtgata cgactaacac aacaggtgat atggcaatga taacacgtca gatgagccat
      361 cgcccactat cgggtggtgc gcatgaaaaa ccctgatctc cagaacctca cggactattc
      421 cccctcagat gcgccctggg acgctcacag aagcgtttct gatgacgtgg gagggattta
      481 cctgcttgca gccgaatatg agcgctacgg ggcgcgtatg gccctctgtg gtggtttact
      541 gcgtttcggg tggtctaccc tcaaggaaac cggagaaacg cgtctgaggc tgcgtgaggc
      601 gcatttctgc cgtgtccggc attgtcctgt ctgccagtgg aggcgctccc tgatgtggca
      661 agcccgtttc taccagtccc tgccgcgtat cgtggcggat tatcccgatg cccgctggat
      721 gttcctgact ctgaccgtgc gcaactgtgc gatcggggag ctgggcgaga tgctgaaccg
      781 gatgaatgca gcttttcagc gcatgaaggt tcgcaaggag ttccggcctg tgcagggctg
      841 gatacgtacc acggaagtca cccgcgggag cgacggcagc gcacacccgc acttccacac
      901 cctgatgatg gtgccgcctg gcatgctaaa tgggaaaagt tacgtcaggc atgaacgctg
      961 ggttgaactc tggcgggagt gtctgcgggt cgattatgac ccgaatgttg atgtccgggc
     1021 agtgaaacct cgtaagccga agggcggcga atcgctggcc tgcgcgaccg ccgaactggt
     1081 gcgcggtgcg gtggccgaaa ccctgaaata ttccacgaag ccggctgaca tggtggccga
     1141 tcctgaatgg ttcctcgaac tgacccgcca gacgcacaag cgccgctttg tggcgaccgg
     1201 cggcgcactc aaagacgtgc tgaaactcga tcaggaaact gacgccgaca tggtgatcgg
     1261 tgatgatatt tccgagggcg acgatgacgg ttcacgcatc gcgttcgagt ggaaaaccga
     1321 ggtcagaaag taccgtcgct caccgacgaa agataaaact gaatcaggct agcggggtgt
     1381 cggggtgcac ccctgaccag atggcaattg tgatagcgtc gcgtgtgacg gtattacaat
     1441 tacacatcct gtcccgaaaa ttcccgccgt ttctcggttt tttgagcgtc gcagaccgca
     1501 cgacaaacga acctaaggtg agtttgtata agtgcgcact tcgtgagatt ccctgactgc
     1561 cagtttgtcg attgcgcgga acggctgcgg cacgaaaacg gcaaaatgat ttactcgccg
     1621 tttgtgtggg ttgttttgtg gggtgtcaat ggggttgtgg tcttttttgt gggttaaagt
     1681 gtgggtgatt gtgtgggctg tgtgttttga gttgtgggtg attatgtggg tcattgtgtg
     1741 ggctgtatgc acggcatttt tttgttctcc taaaacacag gcacgataag cggcgtacac
     1801 aaaacggggc gtttcgctcc gtcgcttgtg ccaaagggga cacccctttg caaccctgtg
     1861 cggttctacg aacctggtca ggactgcgcc ccgacacccc gccaacgcct cacggtacgc
     1921 gaaaaaattc cgcgtaccgt tcaccgttcc ctgattcgtc aaacagaaaa tcgcgggggc
     1981 ccgcgcctcc ggcgtgggtt ccgattttgt gattgacgaa ggcacgggaa aaacactggg
     2041 gatggtggtt ggtgtcacgc agtgaggcca accacctgta agacggtttt ttttgtt
//
