LOCUS       NZ_CP135711             4072 bp    DNA     circular CON 07-OCT-2023
DEFINITION  Escherichia coli strain TUM1857 plasmid pMTY1857_untypable, complete
            sequence.
ACCESSION   NZ_CP135711
VERSION     NZ_CP135711.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN35768948
            Assembly: GCF_032386835.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   Komori,K., Aoki,K., Ishii,Y. and Tateda,K.
  TITLE     Analysis of the Plasmid-Driven Acquisition of blaCTX-M-14 in
            Escherichia coli ST131 Subclone H30
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4072)
  AUTHORS   Komori,K., Aoki,K., Ishii,Y. and Tateda,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2023) Department of Microbiology and Infectious
            Diseases, Toho University, 5-21-16, Omori-Nishi, Ota-Ku, Tokyo
            143-8540, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 13-SEP-2021
            Assembly Method                   :: SPAdes v. v3.15.5
            Assembly Name                     :: Toho-u_TUM1857_Comp_v1.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: missing
            Genome Coverage                   :: 49x
            Sequencing Technology             :: Complete Genomics
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/07/2023 14:52:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,228
            CDSs (total)                      :: 5,120
            Genes (coding)                    :: 4,859
            CDSs (with protein)               :: 4,859
            Genes (RNA)                       :: 108
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 102 of 261
            Pseudo Genes (incomplete)         :: 176 of 261
            Pseudo Genes (internal stop)      :: 52 of 261
            Pseudo Genes (multiple problems)  :: 57 of 261
            Pseudo Genes (short protein)      :: 3 of 261
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP135711.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="TUM1857"
                     /db_xref="taxon:562"
                     /plasmid="pMTY1857_untypable"
                     /geo_loc_name="Japan"
                     /collection_date="2003"
                     /collected_by="Toho University School of Medicine"
     gene            201..902
                     /locus_tag="RS159_RS26130"
                     /old_locus_tag="RS159_26130"
     CDS             201..902
                     /locus_tag="RS159_RS26130"
                     /old_locus_tag="RS159_26130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281395.1"
                     /translation="MSDLIAYKSNALIEASYKLTLQEQRFLLVCISRLKSGEDSPSPDE
                     QKTMTITAAEFHDAFPDMGRQHAEARLKEAIDRLWDRSVIIKNEEKREEFRWVQYRAQY
                     FKGEAKVDITFSDAIMPYLTQLKGQFTRVVVKNVSSLSSTYSIRIYELLQQFRSTGDRT
                     IAIDDFRTMLAVEDKYQQFKDLNKMIIKPAIAELNEKSDLVVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(1160..3307)
                     /gene="mobQ"
                     /locus_tag="RS159_RS26135"
                     /old_locus_tag="RS159_26135"
     CDS             complement(1160..3307)
                     /gene="mobQ"
                     /locus_tag="RS159_RS26135"
                     /old_locus_tag="RS159_26135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_032495499.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPHELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAIGPAGFSGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNGGLTEPARRVADLLNIGLPDNATSYTLRDIIEALPKDSN
                     SAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQMAFEKQWQQWGKSGLAALRAQL
                     LKREEELRRKEQEDTERSRAEQLRKNDNAVMDSGVLRAVVTGYGEAPMPGNGEMTCYLL
                     LHNRNGEYTLWGNELEKYRTRIFESVDLMRDRSGYICDRSEIGQRPPLQRVYSSATFEQ
                     LLTQVCQTWPQHTRNLRQPKTWPESFCLGEDRQPAMPSPAARKVDFMQGRLPPTLMPVM
                     SSVDRETRQLQLLLVMGVDDSLGGVIRLNGTLYPAFAVPSADNRHLVISALTDKGLRYA
                     GYGVAVNHDADSHISPAPELMQFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRTWEEW
                     QREEDARTHTTERRHDRGWSQ"
                     /name="mobQ"
     gene            3486..3740
                     /gene="mobC"
                     /locus_tag="RS159_RS26140"
                     /old_locus_tag="RS159_26140"
     CDS             3486..3740
                     /gene="mobC"
                     /locus_tag="RS159_RS26140"
                     /old_locus_tag="RS159_26140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091746.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPEQ"
                     /name="mobC"
     Typing          complement(2950..3308)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3309..3485
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.438"
                     /coverage="100.0"
                     /evalue="2.610000000000001e-86"
     Typing          202..902
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1164..3394)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.989"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gatgttctgg caagataagt ggagcttttg ggggtgaaag gtggagcttt tgggggtgaa
       61 aggtggagct tttgggggtg aaaggtggag cttttggggg tgtaatccac agaaaaaggg
      121 ggttgcaaaa aaaaaacttc cacccatatg ataagtggcg tacagtaaaa aaacgccacc
      181 aatagcaacg agcaagcgaa atgtcagatc tgatagctta caaatcaaac gcccttatcg
      241 aggcaagcta caagctcact ttgcaggaac agcgcttcct gctggtctgt atcagccgcc
      301 taaaatccgg agaagattcc ccttcgccgg atgaacaaaa aaccatgacc atcacggctg
      361 cagagtttca tgatgccttt cctgacatgg gacggcagca cgctgaagct aggctcaagg
      421 aagcgataga tcgcctctgg gacaggtcag taatcatcaa gaacgaggaa aaacgagaag
      481 agttccgctg ggtgcagtac cgggcgcagt atttcaaagg tgaggcgaaa gtagatatca
      541 ccttctccga tgccatcatg ccgtatctga cacagctcaa ggggcagttt acgcgggtag
      601 tcgtgaagaa cgtctccagc ctttccagca cctacagcat ccgcatttac gagctgctcc
      661 agcagttccg cagcaccggt gacagaacga tagctatcga tgatttccga accatgcttg
      721 ccgttgaaga taaatatcaa caattcaaag acttgaataa gatgataatc aagccagcca
      781 tcgccgaact gaacgaaaaa agcgatctgg ttgtaactgt tgagacgatc aaaaaggggc
      841 gcacggttgt agccctgcat tttcgcttca aggaagacaa acagatcaag atgacgatct
      901 aaaatcgatt ttaaggcacc taaaacgctc cctgctgcgt tttttttgtg ttagtagtgc
      961 gaaggtacta ttgaagtgaa aaattgcgtc aaaaacgctc tgatgcagcg aaaaaagacc
     1021 ccccaatttt tgggggcgga aaaacaaaac tatgcttgcg ttatgtgagt gtgcttcttg
     1081 cattcatccc tgtcgatgaa catcgaagaa catcgacgaa cataacacga tgttaaacaa
     1141 aacaaaatat tgttcacatt cactgtgacc aaccgcgatc atgtctcctc tcggtggtgt
     1201 gtgttcgtgc atcttcctcg cgctgccatt cctcccaggt acgattaaaa cccagcgagc
     1261 ggaacagatg atccggctgt ttttctggtg tgttgacggc agcaaaaagc ggtgcctcac
     1321 gggttttcag atgaaactgc atcagttctg gtgcaggtga tatgtggctg tctgcatcgt
     1381 gatttacggc aacaccatac cctgcatacc gtaacccctt atcggtcagc gcactgatga
     1441 ccagatgcct gttatcggct gagggaacgg caaacgcggg atacagcgta ccattaagcc
     1501 ggataacacc gcccagagag tcatccacgc ccatgaccag caaaagctga agctggcggg
     1561 tttcccggtc gacagaactc atgaccggca tcagcgtcgg tggcagacgt ccctgcataa
     1621 aatccacctt ccttgccgct ggtgatggca tggctggctg gcggtcttct ccaagacaga
     1681 aagactccgg ccatgtcttc ggctggcgga gattgcgggt atgttgcggc catgtctggc
     1741 aaacctgcgt cagcagttgc tcaaaggttg cagaggaata aacacgttgc agtggcggac
     1801 gttggccgat ttcgcttcgg tcacagatat aaccactcct gtcacgcatt aaatcaacac
     1861 tctcaaagat tctggtgcgg tatttctcca gctcgttccc ccagagcgta tattctccgt
     1921 tccggttgtg aagcagaagg taacaggtca tttccccgtt accaggcatg ggagcctcgc
     1981 cataccccgt aacgactgct ctcagcaccc ctgaatccat aacggcattg tcatttttcc
     2041 tgagctgctc cgcgcgacta cgttcagtat cttcctgttc cttccggcgc aattcctctt
     2101 cacgtttaag caactgggct ctgagagctg caaggccgga tttcccccac tgctgccact
     2161 gtttttcaaa tgccatctga ctcgcgggaa tgtcctgttt ctccaccttc cggtaatgct
     2221 gtcgtaattt ttccatttgc tggctcagag actggatttt tccctgctca ctacggtatt
     2281 ccggtgccgc ccattgcatc aggggaatac gggaaaaacc ggacgaaaac ggcgatgatt
     2341 ttttcaggct ggcagcatgg ctcatcgctt cctttcgttc ggtgttcagc tcttcccagc
     2401 gtctgcgcgt cgcctgcata tctgccatca tcgacatggc attactggtg agtttccatg
     2461 ccgagttact gtccttcggc aacgcttcga taatgtcacg tagtgtgtac gaagttgcgt
     2521 tgtcaggaag cccgatattc agaaggtcag ccacacggcg ggcaggctct gtcagccctc
     2581 cgttcctgag ttcggtgcgg gaaatctgta tttccaggtt aagcctgtta atcagacgat
     2641 tctgttcgcc gcgttctgtt tcgataccgc gtgtttccat cgcacaggca gactttccga
     2701 ggtgaatacc aggcgttttc tcaagcccct gtcgctcgta tgaacgatgg tctatctctt
     2761 cctgataacc ggcgttcgcc agtgccctgt ttgcatggtc agcccatgat gcccgccacg
     2821 tctcggcatg tgtccggtcg ttccagtccc tgacctttcc gctgaaccca gcaggtccga
     2881 tagccctcgt ggtcagcatg atgtgcgcat ggggatttgt tttatccatg tgatggaacg
     2941 ccacatcagc aatcatgccc tgactgacaa agttttctct gacaaaggcg agaacggttt
     3001 cccgtgcggc atcctgcggc agttcgtggg ggatggcgag ttcaatttcc cgcgcaagtt
     3061 gtgagttctt ccggcgttcg gccttctcaa cggcattcca gagttcggca cggttcgtac
     3121 accaggcagg ggcggtggcg ggggtcagaa tcacagcctc agccacaccg cttttgcggg
     3181 tgtagtcgtg agtcagtccg gtacgctcat caagcatcag tgaaccggaa cgataggcag
     3241 cggaagctac ggcgctgtag ccgctgcttc tggagataat tttcatgctg agatgataaa
     3301 tcgccatttc tgccgtaatg cctttttgta gttttctttc agccccgcag ggcgcacaca
     3361 atcgaaacgg agtggagatt gtataagtgc gcactttgtg atttgccgtg cattatgcca
     3421 gctcagtttt taaactacca gtgacgcgct aaaatgcgat ttttcatcgc gcacggagtt
     3481 acatcatgag cagaacgctc gaacagaaga ttgctgatgc tgaggcgaga ttacagcgcc
     3541 tgaaggcgaa gagccggagc ctggacacag cgcagaaggt tgttgtgggt gctgcattgc
     3601 tggcaaaggt cagaaagccg gaggaggtgc agttgcgtgc ctggttactc cagttcctga
     3661 aggcagaggt gacacgacag gctgatgtga cgcgcatact gccgctgatt aacgagctgg
     3721 aggcgctgcc agaacagtga ttgtatcgtc cgctcgccgc agccccagcg accgagcgtt
     3781 agcgagcgag tgggcgagga agcggaagtg aatcagtcgc caggttgagc acatacgcac
     3841 ggtttttcgg caggtgatat gcgctgaacc cgcatgaata ctgggtttcc acagggttgg
     3901 agggggtgta ttcgaaactt gcaattctgc tttattgcaa cgcattgttt ttattggttt
     3961 tttgttttca gcaccccaag ttatccacag gctcgccgtt aagtttttaa gatcttattt
     4021 ttagatcttt tttttaaaac ctttttaggt cttgtaacac catgatttta aa
//
