LOCUS       NZ_CP135704             4087 bp    DNA     circular CON 07-OCT-2023
DEFINITION  Escherichia coli strain TUM2367 plasmid pMTY2367_untypable2,
            complete sequence.
ACCESSION   NZ_CP135704
VERSION     NZ_CP135704.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN35768963
            Assembly: GCF_032375265.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Komori,K., Aoki,K., Ishii,Y. and Tateda,K.
  TITLE     Analysis of the Plasmid-Driven Acquisition of blaCTX-M-14 in
            Escherichia coli ST131 Subclone H30
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4087)
  AUTHORS   Komori,K., Aoki,K., Ishii,Y. and Tateda,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2023) Department of Microbiology and Infectious
            Diseases, Toho University, 5-21-16, Omori-Nishi, Ota-Ku, Tokyo
            143-8540, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 13-SEP-2021
            Assembly Method                   :: SPAdes v. v3.15.5
            Assembly Name                     :: Toho-u_TUM2367_Comp_v1.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: missing
            Genome Coverage                   :: 142x
            Sequencing Technology             :: Complete Genomics
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/07/2023 14:33:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,281
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 105
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 87 of 237
            Pseudo Genes (incomplete)         :: 176 of 237
            Pseudo Genes (internal stop)      :: 40 of 237
            Pseudo Genes (multiple problems)  :: 57 of 237
            Pseudo Genes (short protein)      :: 3 of 237
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP135704.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="TUM2367"
                     /db_xref="taxon:562"
                     /plasmid="pMTY2367_untypable2"
                     /geo_loc_name="Japan"
                     /collection_date="2005"
                     /collected_by="Toho University School of Medicine"
     gene            411..1118
                     /locus_tag="RS161_RS26910"
                     /old_locus_tag="RS161_26910"
     CDS             411..1118
                     /locus_tag="RS161_RS26910"
                     /old_locus_tag="RS161_26910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296720.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001296720.1"
                     /translation="MTSNPLIAYKSNALVEASYKLTLQEQRFLLLCISRLNSGTDVASP
                     ELQKTMTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQYRA
                     QYARGEGKAQITFSDAVMPYLTQLKGQFTRVVIKNISNLSRSYSIRIYEILQQFRSTGE
                     RIIALDDFKSSLMLDGKYKDFKTLNRDLIKPCVDELNKKSDLAVTVETIKKGRTVVALH
                     FRFKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(1374..3533)
                     /gene="mobQ"
                     /locus_tag="RS161_RS26915"
                     /old_locus_tag="RS161_26915"
     CDS             complement(1374..3533)
                     /gene="mobQ"
                     /locus_tag="RS161_RS26915"
                     /old_locus_tag="RS161_26915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_023142279.1"
                     /translation="MAIYHLSMKIISRNSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTEYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLLATL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHATERRHDRGWSQ"
                     /name="mobQ"
     gene            3712..3966
                     /gene="mobC"
                     /locus_tag="RS161_RS26920"
                     /old_locus_tag="RS161_26920"
     CDS             3712..3966
                     /gene="mobC"
                     /locus_tag="RS161_RS26920"
                     /old_locus_tag="RS161_26920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091745.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobC"
     Typing          complement(3176..3534)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3535..3711
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          412..1118
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1378..3620)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.599"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cgctgaaccc gcatgaatac tggatttcca cagggttgga gggggtgtat tcggaacttg
       61 caattctgct tttttgcaac gcattgtttt tattggtttt ttgttttcag cactccaagt
      121 tatccacagg ctcgtcgtta agtttttaga tcttgttttt aagatcttgt ttttaatact
      181 tgttttagcg ccaaaattcc ttatagaaaa caaaatgtta caacgctata ggtgatatga
      241 tttaaggtaa aggtgatata atttaaggtg cttgggtgag gtaatttaag gtaaaggtga
      301 ggagatttaa ggtaatattc acaggaaaat ggggggtgaa aatataattg ctcaccaatg
      361 ctaaaggtga taggttttta tttcacctca tttttggcct ggtggttaaa atgacatcaa
      421 accctctgat agcctataag tccaatgcgc tggttgaggc cagttacaag ctaactttac
      481 aagaacagcg tttcttattg ctctgcataa gccgacttaa ctccggtact gacgtagctt
      541 cacctgaatt gcaaaagaca atgaccatta cagctgcgga atattttgat tcattccctg
      601 atatggggcg gaaaaatgcc gaggttcagc tacaggaagc gatcgatcgt ttgtgggaca
      661 ggtcgatcat tctcaaggac gacgagaagc gcgaggagtt ccgctggatt cagtatcggg
      721 cacagtacgc gaggggagag gggaaagcac aaatcacttt ttctgacgcg gtaatgcctt
      781 atctgacaca gttgaagggg caattcacca gagtcgtcat taaaaatatt tctaatttat
      841 caagaagtta cagcataaga atatacgaaa ttctccagca attccgcagt actggggaac
      901 gtattatcgc cctcgatgat tttaaatcat cactgatgct tgatggtaaa tataaagatt
      961 tcaaaacgtt aaacagggat ctaataaaac cctgtgttga cgaactgaac aaaaaaagcg
     1021 acctggctgt aacggttgag acgatcaaaa aggggcgcac ggttgtagcc ctgcattttc
     1081 gcttcaagga agacaaacag atcaagatga cgatctagaa tcgattttaa ggcacctaaa
     1141 acgctccctg ctgcgttttt tttgtgttag tagtgcgaag gtactattga agtgaaaaat
     1201 tgcgtcaaaa gcgctctaat gcagcgaaaa aagaaccccc atttttcggg ggcggaaaaa
     1261 caaaactatg cttgcattat gtgtgtgctt cttgcattca tccctgtcga tgaacatcga
     1321 agaacatcga cgaacataac acgatgttaa acaaaacaaa atattgttca cattcactgt
     1381 gaccaaccgc gatcatgtct cctctcggtg gcgtgtgttc gtgcatcttc ctcgcgccgc
     1441 cattcgtccc aggtacgatt aaaacccagc gagcggaaca gatgatccgg ctgtttttct
     1501 ggtgtgttga cggcagcaaa aagcggtgcc tcacgggttt tcagatgaaa ctccataagt
     1561 tccggcgcag gtgatatgtg gctgtctgca tcgtgattta cggcaacacc ataccctgca
     1621 taccgtaacc ccttatcggt cagtgcactg atgaccagct ggctgttatc ggctgaggga
     1681 acggcaaacg cgggatacag cgtaccatta agccggacaa caccgcccag agagtcatcc
     1741 acgcccatga ccagcaaaag ctgaagctgg cgggtttccc ggtcgacaga actcattacc
     1801 ggcatcagcg tcgctagcag acgtccctgc gtaaaatcca ccttccttgc tgccagtgat
     1861 ggcatggctg gctggcggtc ttctccaaga cagaaagact ccggccatgt cttcggctgg
     1921 cggagattgc gggtatattg cggccatgtc tggcaaacct gcgtcagaag ttgctcaaag
     1981 gtcgcagagg aataaacacg ttgcagtggt ggatgttgac cgatttcgct tcggtcacag
     2041 atataaccac tcctgtcacg cattaaatca acacgctcaa agattctggt gcggtatttc
     2101 tccagctcgt tcccccagag cgtgtattct ccgttccggt tgtgaagcag aaggtaacag
     2161 gtcatttccc cgttaccagg catgggggcc tcgccatact ccgtaacgac tgcgctcagt
     2221 acccctgaat ccataacggc attgtcattt ttcctgagct gttccgctcg tcgctccgcg
     2281 cgactacgtt ctgtatcctc ctgctccttc cggcgcaatt cctcttcacg tttaagcaac
     2341 tggactctga gagctgcaag gccggatgcc ccccactgct gccactgttt ttcaaatgcc
     2401 gtctgactcg cgggaatgtc ctgtttctcc accttccggt aatgctgtcg caatttttcc
     2461 atttgctggc tcagcgactg gatttttccc tgttcgctac ggtattccgg tgccgcccat
     2521 tgcatcaggg gaatacggga aaaaccggac gaaaacggcg atgatttttt caggctggca
     2581 gcatggctca tcgcttcctt tcgttcggtg ttcagctctt cccagcgtct gcgcgtcgcc
     2641 tgcatatctg ccatcatcga catggcatta ctggtgagtt tccatgccgc attactgtcc
     2701 ttcggcaacg cttcgatgat gtcacgtagt gtgtacgaag ttgcgttgtc aggaagcacg
     2761 atattcagaa ggtcagccac acggcgggca ggctctgtca gcccgctgtt tctgagttcg
     2821 gtacgggaaa tctgtatttc caggttaagt ctgttaatca gacgattctg ttcgccgcgt
     2881 tctgtttcga taccgcgtgt ttccatcgca caggcagact ttccgaggtg aataccaggc
     2941 gttttctcaa gcccctgtcg ctcgtatgaa cgatggtcta tctcttcctg ataaccggcg
     3001 ttcgccagtg ccctgttcgc atggtcagcc catgatgcac gccacgtctc ggcatgtgtc
     3061 cggtcgttcc agtccctgac ctttccaccg aatccagcag gtccgacagc tctcgtggtc
     3121 agcatgatgt gcgcatgggg atttgtttta tccatgtgat ggaacgccac atcagcaatc
     3181 atgccctgac tgacaaagtt ttctctgaca aaggcgagaa cggtttcccg tgcggcatcc
     3241 tgcggtagtt cgcgggggat ggcgagttca atttcccgcg caagttgtga gttcttccgg
     3301 cgttcggcct tctcaacggc attccagagt tcggcacggt tcgtacacca ggcaggggct
     3361 gtcgcgggag tcagaatcac agcttcagcc acaccgcttt tgcgggtgta gtcgtgagtc
     3421 agtccggtac gctcatcaag catcagtgaa ccggaacgat aggcagcaga agcaacggcg
     3481 ctgtagccgc tgtttctgga gatgattttc atgctgagat gataaatcgc catttctgcc
     3541 gtaatgcctt tttgtggttt tctttcagcc ccgcagggcg cacacaatcg aaacggagtg
     3601 gagattgtat aagtgcgcac ttcgtgattt tccgtgcatt atgccagctc agtttttaaa
     3661 ctaccagtga cgcgctaaaa tgcgattttt catcgcgcac ggagttacat catgagcaga
     3721 acactcgaac agaagattgc tgatgctgag gccagattac agcgcctgaa ggcgaagagc
     3781 aggagtctgg acacagcgca gaaggtcatt gtgggtgcag cattgctggc aaaggtcaga
     3841 aagccggagg aggtgcagtt gcgtgcctgg ttactccagt tcctgaaggc agaggtgaca
     3901 cgacaggctg atgtgacgcg catactgccg ctgattaacg agctggaagc gctgccagga
     3961 cagtgattgt atcgtccgct cgccgcagtc ccagcgaccg agcgttagcg agcgagtggg
     4021 cgaggaagcg gaagtgaatc cgtcgcctga ttgagcacat acgcacggtt tttcgggagg
     4081 tgatatg
//
