LOCUS       NZ_CP135702             5251 bp    DNA     circular CON 07-OCT-2023
DEFINITION  Escherichia coli strain TUM2367 plasmid pMTY2367_Col1, complete
            sequence.
ACCESSION   NZ_CP135702
VERSION     NZ_CP135702.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN35768963
            Assembly: GCF_032375265.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5251)
  AUTHORS   Komori,K., Aoki,K., Ishii,Y. and Tateda,K.
  TITLE     Analysis of the Plasmid-Driven Acquisition of blaCTX-M-14 in
            Escherichia coli ST131 Subclone H30
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5251)
  AUTHORS   Komori,K., Aoki,K., Ishii,Y. and Tateda,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2023) Department of Microbiology and Infectious
            Diseases, Toho University, 5-21-16, Omori-Nishi, Ota-Ku, Tokyo
            143-8540, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 13-SEP-2021
            Assembly Method                   :: SPAdes v. v3.15.5
            Assembly Name                     :: Toho-u_TUM2367_Comp_v1.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: missing
            Genome Coverage                   :: 142x
            Sequencing Technology             :: Complete Genomics
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/07/2023 14:33:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,386
            CDSs (total)                      :: 5,281
            Genes (coding)                    :: 5,044
            CDSs (with protein)               :: 5,044
            Genes (RNA)                       :: 105
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 87 of 237
            Pseudo Genes (incomplete)         :: 176 of 237
            Pseudo Genes (internal stop)      :: 40 of 237
            Pseudo Genes (multiple problems)  :: 57 of 237
            Pseudo Genes (short protein)      :: 3 of 237
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP135702.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5251
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="TUM2367"
                     /db_xref="taxon:562"
                     /plasmid="pMTY2367_Col1"
                     /geo_loc_name="Japan"
                     /collection_date="2005"
                     /collected_by="Toho University School of Medicine"
     gene            complement(607..819)
                     /locus_tag="RS161_RS26850"
                     /old_locus_tag="RS161_26850"
     CDS             complement(607..819)
                     /locus_tag="RS161_RS26850"
                     /old_locus_tag="RS161_26850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137423.1"
                     /translation="MTELEKQLLTALEQLHQDYSQRLDEWESAFAEWQKMSGLMQRENA
                     ALNERVTRLSQQVANLSRQLQRLSQ"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(823..1308)
                     /locus_tag="RS161_RS26855"
                     /old_locus_tag="RS161_26855"
     CDS             complement(823..1308)
                     /locus_tag="RS161_RS26855"
                     /old_locus_tag="RS161_26855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_013631087.1"
                     /translation="MSSLLALAKDLEQQSKAQQQSTGEMLKAAFSEHEQSVKAELSASA
                     KRISDAISAHEQGMTAAMQSNRLSVLRMVGRTWLTITLVSVLLIATSGSILWWQGQQIT
                     GNYQTIRAQERTQAMLSEKNHGVQLSLCGEKKLSCVKVNPKAGAYGEEGNWMVLERK"
                     /name="MbeB family mobilization protein"
     gene            complement(<1346..2023)
                     /locus_tag="RS161_RS26860"
                     /old_locus_tag="RS161_26860"
                     /pseudo=""
     CDS             complement(<1346..2023)
                     /locus_tag="RS161_RS26860"
                     /old_locus_tag="RS161_26860"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1986..2306)
                     /locus_tag="RS161_RS26865"
                     /old_locus_tag="RS161_26865"
     CDS             complement(1986..2306)
                     /locus_tag="RS161_RS26865"
                     /old_locus_tag="RS161_26865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_014386475.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGRLPSI
                     SPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAVLEKGGDD
                     DR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2943..3104
                     /locus_tag="RS161_RS26870"
                     /old_locus_tag="RS161_26870"
     CDS             2943..3104
                     /locus_tag="RS161_RS26870"
                     /old_locus_tag="RS161_26870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023144844.1"
                     /translation="MHEPPVQSDRCAFSGNYHLESNPERHGSSPLAAAIGNGLEEVSLE
                     VLRQLRLN"
                     /name="hypothetical protein"
     gene            complement(3257..3877)
                     /locus_tag="RS161_RS26875"
                     /old_locus_tag="RS161_26875"
     CDS             complement(3257..3877)
                     /locus_tag="RS161_RS26875"
                     /old_locus_tag="RS161_26875"
                     /inference="COORDINATES: protein motif:HMM:NF025084.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RloB family protein"
                     /protein_id="WP_023312615.1"
                     /translation="MSKKHGNNKKVPQVMYIYCEGEKTEPLYLEAYISDRSKRTLSVFK
                     IPKTRKNTPEQLVDEAIKKKNSSSSAEGDVFWVVYDQEHLTPSSISCHQKAWNKANRHG
                     INIAISCVCFELWLLLHFGFTTKDFSSYDNLMSSSPLKKLITNYDKGSKLTYLAIRERI
                     GTAKDNARKLDIYAKTNYKNTHEPYLFPCYTSFHELLKAIDDF"
                     /name="RloB family protein"
     gene            complement(3870..5225)
                     /locus_tag="RS161_RS26880"
                     /old_locus_tag="RS161_26880"
     CDS             complement(3870..5225)
                     /locus_tag="RS161_RS26880"
                     /old_locus_tag="RS161_26880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498067.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_046380597.1"
                     /translation="MFVYLTIENFRSVKEKSTLNLSASGSSNHLTTHIYKNDGMNVGTL
                     MSAGIYGANASGKSNVLMAFEAIKFIATDSGDLKEGKRIPCYEPYALSSATNEAPVKFE
                     AEFYSVEGNRFSYEVAFIKNRILFESLDYYPSRVKANLFTRDESDTWETIKFGGHYKGG
                     IKKIPFFPNNSYLAKAGNNAASPDIIKEAYNFFRKGIRHIGLNEKIRISSFGEREDVVN
                     ETAKVLCMVDTGVSGISVREVEAEIPIKMDEEIPQEIKEIIEEDYKYRYMFSHPTDEGG
                     SVSFPMGRESEGTQKLFEIIPLIRSAFNDSMVVIVDELDNSLHPHIADLIVKLFNDPDV
                     NKKGSQLVFSTHNMQLMAPEKMRRDQIWFCEKNKGASSLYSLDDFDKKKIKTTTPYAAW
                     YDEGRFGGVPDINYLKVASFISGDISLVMPDIDVKELSDGFFEEFDGDLSDE"
                     /name="ATP-binding protein"
     Typing          complement(2421..2593)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="84.659"
                     /coverage="100.0"
                     /evalue="9.74e-41"
     Typing          2540..3053
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.617"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(492..1996)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.215"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ccaaaaaagc gaaaaatcaa tcataggaaa gatatcctat atgttttaat tttggaacaa
       61 catcttcctt aaaaaacgtt gtgctattta taattacacg ctatttacca taacatctgt
      121 tgcccgcacc gctccggaca ggttcgccac aaacggggcg gcagtcaggt aaaaccggca
      181 acaaaccacc cgatacaccg ccccgttcgt gacagataaa ttttacccgg ctgcatggtc
      241 atgcagccag atgcatacgg tctgatttca ggccagaatc cggatgaacg ccttaaaaac
      301 aggaaaatgc ttctccagcc agtcccagat ctccagcgcc atgtacaggc aattcagcac
      361 cgtctcaatc acgttaaaca gtctcataat ccctccgttg tatacgctgt gatggaaaca
      421 tcacgaccgt atttagcggg aaatttatga gcccttcttc ttggggtttg tcactggatg
      481 cgcgcagtca gcccagcgtc ggaccgtcat agctccgctg ccgggaacgg gtttccgcca
      541 gctctttttc atgctgtttc tgctcgtatg cccgttcggc ggccaccgcc atttcatgcc
      601 gctgcactac tggctcaaac gctgtaactg cctgctcaag ttcgccacct gctgactcaa
      661 gcgcgtgacg cgctcgttca gcgccgcgtt ctcccgttgc ataagaccag acatcttctg
      721 ccattccgcg aaggcgctct cccactcgtc cagcctttgc gagtagtcct gatgtagctg
      781 ctctaatgcg gtcagcaact gtttttccag ttccgtcatg ggctatttcc tctccagtac
      841 catccagttc ccttcctccc cgtaagctcc cgctttcggg ttcactttga cgcaggacag
      901 tttcttctcc ccgcagagcg acagctgcac gccgtggttc ttctccgaca gcatggcctg
      961 cgtccgctcc tgcgcccgga tagtctggta attcccggtg atctgctgcc cctgccacca
     1021 cagaatgctc ccgctcgtgg cgatcagcag taccgagacc agggtgatgg tcagccacgt
     1081 ccggccgacc atacgcagca cgctcagccg gttcgactgc atcgccgcgg tcataccctg
     1141 ctcgtgggcg ctgatggcgt cgctgattct cttcgcgctt gcgctcagtt ccgcttttac
     1201 agactgctcg tgctcgctga atgcggcttt cagcatctcg ccggtactct gctgctgcgc
     1261 tttcgattgc tgctccaagt ccttcgccag cgctaaaaga ctgctcataa atggctccct
     1321 tcagtcggat gtttcgcccc ccgtccgggt cggcaatgct gatactgttt tttgtggtgc
     1381 ggaccacctc aaaacctgcc gctgtgagcg cccctgtgac gtcctcgcgc gttttgagct
     1441 ccccggatga ggctaaggcc agcaacccac gtgtaatcgc ctgagcggct tccagcttcg
     1501 tttccggcag tgcggacggc gtgaccagta accgtcggtt ctccggggcg tttggatcat
     1561 gcagccccag cctgccgttc accacggtct gccaggcatc gatgcgcggc cggtcggcgc
     1621 gatcgtagta cggctgcagg cgtttcccgg tcagcagttc agtgttgggg atcaggaaat
     1681 tcagctccag ccgccccttg tcggtgtgct ccacccacag aatgctgtac tggtctttat
     1741 cgagcccggg catcagaacc cgctcaaagc tggccatgat ctgctcccgc tgcccgggcg
     1801 gcagttcagc ctccgcgaac gacagcacgc cggaggtgta ctttttcgca tacggcgagg
     1861 catcgataag ctcccggact tcctccggtt ttccctgcag cacggtcgcc ccctcgcgct
     1921 gccggtcttt tcccagcagg taatccaccg gaccggcacc accaccgcgc ccccgggggt
     1981 gaaatttaac gatcatcatc cccccctttt tccagcaccg catgccgcag ccgctccagt
     2041 cccgcgtcga ttgccatcag tgcggccaca atctgcaccc ggtcatgacc ggcaccaccg
     2101 gcattcactt tccgggcgat ctggttcagg ttgttcccca taccggcaag ctggcgcagc
     2161 agcgccggcg atatcgacgg cagtctgccg gcacgcgccg gcttctcatc gaggcaggtc
     2221 tgacgcatcc acgccgccag ctgcttgccg tcgcaccgct cgagcagccg ccggtgttcg
     2281 tcttccgtca cccacatcgt gagcatcttg ttgcgtttgt ctgccagagt agcctcctga
     2341 taacaccggg acgggcggga aatgtttccc ggtcggcacg gtgttggaca agccgcaggc
     2401 gcgtcagtcg cttttagcgg gtttcggggc gcagccctga accagtcacg tagcgctagc
     2461 ggagtgtata ctggcttatt atgttggcat gagtgcggta atcaggaaag tgcttcatgt
     2521 gacagaggaa aatgcagctg cgggcgtaag gcagaaaatg gaagttcaga ggatatgacg
     2581 cctcctcgct cactcagtcg ctgccgctcc tgccgttcgg ctgcggcggg cgttacctgc
     2641 acacggagaa gagcgaaggc atatggcatg acaaacgggg cagaaaaagg atgcgccgct
     2701 gccgtttttc cataggctcc gcccccctga caagcatcac gaaatctgac gctcaaatca
     2761 gtggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gtggctccct
     2821 cgtgcgctct cctgttcctg cctttcggtt taccggtgtc attccgctgt tatggccgcg
     2881 tttgtctcat tccacgcctg acactcagtt ccgggtaggc agttcgctcc aagctggact
     2941 gtatgcacga accccccgtt cagtccgacc gctgcgcctt ctccggtaac tatcatcttg
     3001 agtccaaccc ggaaagacac ggttcatcgc cactggcagc agccattggt aacggattag
     3061 aggaagtgag tcttgaagtt ctgcgccagt taaggctaaa ctgaaaggac aatttttggt
     3121 gactgcgctc cctctaagcc agttacccgg tacataagtt ggtagctcat gtaccttcca
     3181 aaaaccaccg ttggtagcgg tggtttttat ttttcataga ttacagcctg taataagcca
     3241 tggtcaatgg attacttcaa aagtcgtcaa tcgccttaag tagctcatgg aatgatgtat
     3301 aacatggaaa tagataaggt tcgtgcgtgt ttttataatt agttttagcg taaatatcta
     3361 gctttcttgc gttatctttc gcagtaccaa tcctctctct tatagcaagg taagttagtt
     3421 tactaccttt atcatagtta gttattaatt tttttagcgg ggagctagac atcaaattat
     3481 catacgacga aaaatctttg gttgtgaagc cgaaatgcaa caataaccat aattcaaaac
     3541 aaacacagga tattgctata tttatcccat gcctattagc tttattccat gccttttgat
     3601 gacatgaaat agacgaaggc gtcagatgct cttgatcata aacaacccaa aagacgtcac
     3661 cttctgcgga agatgatgaa ttctttttct ttatcgcctc atccaccaac tgctcaggtg
     3721 tattttttct tgttttaggt attttaaata cacttaaagt tcttttagat ctgtctgaaa
     3781 tataagcctc aaggtataat ggttcggttt tctcaccctc acagtatata tacattactt
     3841 gtgggacttt cttattattt ccatgttttt tactcatcag aaagatcccc gtcaaactct
     3901 tcaaaaaaac catcagacaa ttctttgacg tcaatatccg gcattaccaa ggatatatca
     3961 ccagatataa atgaggctac ctttaagtag tttatgtcgg gaaccccacc aaacctaccc
     4021 tcatcatacc aagctgcgta aggtgttgtt gtttttatct ttttcttatc aaaatcatct
     4081 aagctataaa gagacgacgc acctttattt ttttcacaaa accatatctg atctcttctc
     4141 attttctctg gagccattaa ctgcatatta tgggttgaga atacaagttg tgagcctttt
     4201 ttattgacat caggatcatt aaataactta acaatcaagt cagcaatatg cgggtgcaag
     4261 ctattatcta actcatcaac aattacaacc atactatcat taaacgcaga tctaattaga
     4321 ggtatgattt caaataattt ttgggtgcct tcagactctc tccccatagg gaaagaaaca
     4381 ctcccccctt catcagtagg atgagagaac atataacgat atttataatc ttcttcaatt
     4441 atctccttta tttcttgagg gatttcctca tccattttta ttggtatttc cgcctcaacc
     4501 tcccgtacag atataccgga aacaccagtg tcaaccatgc ataaaacctt ggccgtttca
     4561 tttacgacgt cctccctctc cccaaaagaa gatattctta tcttttcatt taaacctata
     4621 tgtctaattc cctttctaaa aaagttataa gcttctttaa ttatgtctgg tgatgcggca
     4681 ttattacctg ctttcgctaa gtatgaatta ttagggaaga atggtatttt tttaatacct
     4741 cctttataat gcccaccaaa cttgattgtt tcccaggtgt cgctctcatc tctagtaaat
     4801 aaatttgctt taaccctaga tggataatag tctaatgatt caaataaaat ccgattttta
     4861 ataaaagcaa cttcataact gaaccgattc ccctcaacag aataaaattc agcctcaaac
     4921 tttacaggag cctcattcgt agccgaagaa agagcatatg gctcatagca gggaatccgt
     4981 ttaccttctt tcaaatcacc gctatctgtt gcgataaatt ttatagcttc aaaagccatg
     5041 agaacgttag atttaccaga cgcattagca ccataaatcc cagcactcat gagcgttcca
     5101 acattcattc catcattttt atagatatga gttgttaaat ggttactaga accggatgcg
     5161 ctcaagttta atgtcgattt ttccttaacc gacctaaaat tttcaatagt caaataaaca
     5221 aacatgcata cctcctaacg gggatttttc g
//
