LOCUS       NZ_CP135676             6756 bp    DNA     circular CON 07-OCT-2023
DEFINITION  Escherichia coli strain TUM9803 plasmid pMTY9803_Col1, complete
            sequence.
ACCESSION   NZ_CP135676
VERSION     NZ_CP135676.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN35769040
            Assembly: GCF_032382315.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6756)
  AUTHORS   Komori,K., Aoki,K., Ishii,Y. and Tateda,K.
  TITLE     Analysis of the Plasmid-Driven Acquisition of blaCTX-M-14 in
            Escherichia coli ST131 Subclone H30
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6756)
  AUTHORS   Komori,K., Aoki,K., Ishii,Y. and Tateda,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2023) Department of Microbiology and Infectious
            Diseases, Toho University, 5-21-16, Omori-Nishi, Ota-Ku, Tokyo
            143-8540, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 13-SEP-2021
            Assembly Method                   :: SPAdes v. v3.15.5
            Assembly Name                     :: Toho-u_TUM9803_Comp_v1.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: missing
            Genome Coverage                   :: 179x
            Sequencing Technology             :: Complete Genomics
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/07/2023 15:02:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,120
            CDSs (total)                      :: 6,000
            Genes (coding)                    :: 5,606
            CDSs (with protein)               :: 5,606
            Genes (RNA)                       :: 120
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 394
            CDSs (without protein)            :: 394
            Pseudo Genes (ambiguous residues) :: 0 of 394
            Pseudo Genes (frameshifted)       :: 148 of 394
            Pseudo Genes (incomplete)         :: 272 of 394
            Pseudo Genes (internal stop)      :: 84 of 394
            Pseudo Genes (multiple problems)  :: 93 of 394
            Pseudo Genes (short protein)      :: 4 of 394
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP135676.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6756
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="TUM9803"
                     /db_xref="taxon:562"
                     /plasmid="pMTY9803_Col1"
                     /geo_loc_name="Japan"
                     /collection_date="2010"
                     /collected_by="Toho University School of Medicine"
     gene            join(6101..6756,1..1564)
                     /gene="mobQ"
                     /locus_tag="RS166_RS30520"
                     /old_locus_tag="RS166_30520"
     CDS             join(6101..6756,1..1564)
                     /gene="mobQ"
                     /locus_tag="RS166_RS30520"
                     /old_locus_tag="RS166_30520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_121832145.1"
                     /translation="MAIYHLSMKIISRKNGYSAVASAAYRSGSVIPDDRTGLTHDYTRK
                     RGVDDAVILTPVNAPSWCGDRSVLWNAVEKAEQRRNSQLAREIELAIPREISREAAREA
                     VLAFVRENFVSRGMIADVAFHHMDRTNPHAHIMLTTRAVGETGFAGKVRDWNDRALAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKAPGLHLGKAACAMEKRGMETERGEQ
                     NRLINSLNLEIQVSRTRLAVIQVQENVRKRELSDAARRAAEALNLTIPAANASADTLRE
                     FIATLPQECGNAWEMTPEFVAMNGKANDIKREGNALVKERDILEKEMTGLRKARPVASI
                     LSELPLMTWAEPEYRKRQLRFWKLEKQIESLLRTYRAVKERDIPARRQAFETQWNTWIS
                     PGMAELKEKLSAREAERRREEAEAEARRKEQEYDARLKHHDNHRLSRETALAGVITGLG
                     RAREPGTGRITRYMMLSNRAGEFTVWGDELAHYPQSVHDPVNVYLSPGGAVMVTGIHSG
                     LSESHETLARPERVRMYSGATVRHVLEQMRQGWPSYGFPALPHHWPDNFYFSDDRRPAA
                     SPLPSAHRVDVTAYAAPEQLMPVVFSTERNSRTLNLLLCEGPEEMLVGFVRLEDELRPV
                     LALPSPDYSHLMVSIITENGIHLAGYGEAINRDADTPYPPEPKLMQFRLKGCQDTLFAA
                     INKPEEMPDYLFRQLGFNQTWHEWKREEQRRQQQRRPGHFRGMSM"
                     /name="mobQ"
     gene            1796..3544
                     /gene="col"
                     /locus_tag="RS166_RS30525"
                     /old_locus_tag="RS166_30525"
     CDS             1796..3544
                     /gene="col"
                     /locus_tag="RS166_RS30525"
                     /old_locus_tag="RS166_30525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012644886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-E9"
                     /protein_id="WP_121832143.1"
                     /translation="MSGGDGRGHNTGAHSTSGNINGGPTGIGVSGGASDGSGWSSENNP
                     WGGGSGSGIHWGGGSGRGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA
                     VSISASELSAAIAGIIAKLKKVNLKFTPFGVVLSSLIPSEIAKDDPNMMSKIVTSLPAD
                     DITESPVSSLPLDKATVNVNVRVVDDVKDERQNISVVSGVPMSVPVVDAKPTERPGVFT
                     ASIPGAPVLNISVNNSTPAVQTLSPGVTNNTDKDVRPAGFTQGGNTRDAVIRFPKDSGH
                     NAVYVSVSDVLSPDQVKQRQDEENHRQQEWDATHPVEAAERNYERARAELNQANEDVAR
                     NQERQAKAVQVYNSRKSELDAANKTLADAIAEIKRFNRFAHDPMAGGHRMWQMAGLKAQ
                     RAQTDVNNKQAAFDAAAKEKSDADAALSAAQERRKQKENKEKDAKDKLDKESKRNKPGK
                     ATGKGKPVGDKWLDDAGKDSGAPIPDRIADKLRDKEFKNFDDFRRKFWEEVSKDPELSK
                     QFNPGNKKRLSQGLAPRARNKDTVGGRRSFELHHDKPISQDGGVYDMDNLRITTPKRHI
                     DIHRGQ"
                     /name="col"
     gene            3547..3804
                     /locus_tag="RS166_RS30530"
                     /old_locus_tag="RS166_30530"
     CDS             3547..3804
                     /locus_tag="RS166_RS30530"
                     /old_locus_tag="RS166_30530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_000421100.1"
                     /translation="MELKNSISDYTETEFKKIIEDIINCEGDEKKQDDNLEHFISVTEH
                     PSGSDLIYYPEGNNDGSPEAVIKEIKEWRAANGKSGFKQG"
                     /name="bacteriocin immunity protein"
     gene            3866..4009
                     /locus_tag="RS166_RS30535"
                     /old_locus_tag="RS166_30535"
     CDS             3866..4009
                     /locus_tag="RS166_RS30535"
                     /old_locus_tag="RS166_30535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001314446.1"
                     /translation="MKKITGIILLLLAVIILAACQANYIRDVQGGTVSPSSTAEVTGLA
                     TQ"
                     /name="colicin release lysis protein"
     gene            4538..5431
                     /locus_tag="RS166_RS30540"
                     /old_locus_tag="RS166_30540"
     CDS             4538..5431
                     /locus_tag="RS166_RS30540"
                     /old_locus_tag="RS166_30540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001277133.1"
                     /translation="MSAVLQRFREKLPHKPYCTNDFAYGVRILPKNIAILARFIQQNQP
                     HALYWLPFDVDRTGASIDWSDRNCPAPNITVKNPRNGHAHLLYALALPVRTAPDASASA
                     LRYAAAIERALCEKLGADVNYSGLICKNPCHPEWQEVEWREEPYTLDELADYLDLSASA
                     RRSVDKNYGLGRNYHLFEKVRKWAYRAIRQGWPVFSQWLDAVIQRVEMYNASLPVPLSP
                     AECRAIGKSIAKYTHRKFSPEGFSAVQAARGRKGGTKSKRAAVPTSARSLKPWEALGIS
                     RATYYRKLKCDPDLAK"
                     /name="replication initiation protein"
     gene            complement(5647..5922)
                     /locus_tag="RS166_RS30545"
                     /old_locus_tag="RS166_30545"
     CDS             complement(5647..5922)
                     /locus_tag="RS166_RS30545"
                     /old_locus_tag="RS166_30545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000091752.1"
                     /translation="MSRTLEQKIAEAEARLQRLKAKSRSLDTAQKVIVGAAMLARVRRP
                     EEAQLRAFLLQFLRKEVTRQADVNRLQPLINELEKLPRPPAKPQNH"
                     /name="hypothetical protein"
     BGC             1..6756
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(5927..6100)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="94.857"
                     /coverage="100.0"
                     /evalue="2.61e-71"
     Typing          complement(6101..6459)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.889"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4539..5431
                     /name="rep_cluster_2150"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.275"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3..1561
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="88.654"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          6102..6754
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.624"
                     /coverage="99.0"
                     /evalue="3.36e-134"
ORIGIN
        1 cggtgagcag aaccgcctga ttaacagcct taacctggaa atacaggttt cccgcacaag
       61 gctggcagtc atccaggtac aggagaacgt gcggaaacgg gaactcagcg atgccgcacg
      121 tcgtgcagcg gaagccctta atctgaccat tcccgccgcg aatgcctcag cggataccct
      181 gcgggaattc attgccacac tgccgcagga atgcgggaac gcatgggaga tgacaccgga
      241 gttcgtggcg atgaacggga aggcaaacga catcaaacgt gaggggaatg cgctggtgaa
      301 agagcgggat attctcgaaa aagagatgac cggactcaga aaagcccgtc cggtagcgtc
      361 catcctgtca gagcttcccc tgatgacatg ggctgaaccg gaataccgca aaagacaact
      421 ccgcttctgg aaactcgaga aacagattga atctcttctc cgcacctaca gggccgtgaa
      481 agaacgggac attcccgccc gtcgtcaggc ctttgaaacg cagtggaata cgtggatttc
      541 gccgggaatg gcagagctga aagaaaaact gtcagcacgg gaagcggagc ggcgcaggga
      601 ggaagccgaa gcggaagcgc gccggaagga acaggaatat gacgcacggc tgaaacacca
      661 tgataaccat cgcctgagcc gtgaaacggc attagccggg gttattacgg ggctggggcg
      721 tgccagagag ccgggaacgg gcaggataac ccgctacatg atgctgagta acagagccgg
      781 agaattcacg gtatggggtg atgagctggc gcattacccc cagagtgttc atgacccggt
      841 gaatgtttac ctgtcgccag gtggggccgt gatggtcacg ggtatacatt cggggctgtc
      901 agaatctcat gagacgctgg cccggcctga gcgtgtgaga atgtattccg gtgcgacggt
      961 ccggcatgta ctggaacaga tgcgccaggg gtggccctct tacggttttc cggcgctgcc
     1021 gcatcactgg ccggataatt tttatttcag cgacgaccgc aggcccgcag cctctccgct
     1081 gccgtcagcg caccgggtgg acgtcaccgc ttatgcggca ccggagcaac tcatgcccgt
     1141 tgtcttttcg acagagcgaa acagcaggac gctgaatctg ctgttgtgcg aagggccgga
     1201 ggaaatgctt gtcggttttg tgcgcctgga ggacgagctg cgtcccgttc ttgcgcttcc
     1261 gtcgccggat tacagtcatc tgatggtaag catcatcaca gagaacggga tacacctggc
     1321 aggttacggt gaggccataa accgtgatgc tgatactccg tacccaccgg aaccgaagct
     1381 gatgcagttc aggcttaagg gctgtcagga cacgcttttt gctgccatca acaaaccgga
     1441 agagatgccg gattatctct tccgtcaact cggatttaat cagacctggc atgagtggaa
     1501 gcgggaggaa cagcgcaggc aacaacaacg ccgtcccggg cacttccggg gcatgagtat
     1561 gtgatgtccg gggctgcact ccggaccccg ccaacacatc acgggccaca aaattttttg
     1621 tggcccgctc tgcgttttct aagtgttatc cctcctgact tctaaaaaat tttccacctg
     1681 aacttgacag aaaaaacgat gacgggtact ttttgatctg tacataaaac cagtggtttt
     1741 atgtacagta ttaatcatgt aattaattgt tttaacgctt aaaagaggga cttttatgag
     1801 cggtggagat ggacgcggcc ataacacggg cgcgcatagc acaagtggta acattaatgg
     1861 tggcccgacc gggattggtg taagtggtgg tgcttctgat ggttcaggat ggagttcgga
     1921 aaataacccg tggggtggtg gttccggtag cggcattcac tggggaggtg gctccggtcg
     1981 tggtaatggc gggggtaatg gcaattccgg tggtggctcg ggaacaggcg gtaatttgtc
     2041 agcagtagct gcgccagtgg catttggttt tccggcgctt tccactccag gagctggcgg
     2101 tctggctgtc agtatttctg caagcgaatt atcggcagct attgctggta ttattgctaa
     2161 attaaaaaaa gtaaatctta aattcactcc ttttggggtt gtcttatctt cattaattcc
     2221 gtcggaaata gcgaaagatg accccaatat gatgtcaaag attgtgacgt cattacccgc
     2281 agatgatatt actgaatcac ctgtcagttc attacctctc gataaggcaa cagtaaacgt
     2341 aaatgttcgt gttgttgatg atgtaaaaga cgaacgacag aatatttcgg ttgtttcagg
     2401 tgttccgatg agtgttccgg tggttgatgc aaaacctacc gaacgtccag gtgtttttac
     2461 ggcatcaatt ccaggtgcac ctgttctgaa tatttcagtt aataacagta cgccagcagt
     2521 acagacatta agcccaggtg ttacaaataa tactgataag gatgttcgcc cggcaggatt
     2581 tactcagggt ggtaatacca gggatgcagt tattcgattc ccgaaggaca gcggtcataa
     2641 tgccgtatat gtttcagtga gtgatgttct tagtcctgac caggtaaaac aacgtcagga
     2701 tgaagaaaat caccgtcagc aggaatggga tgctacgcat ccggttgaag cggctgagcg
     2761 aaattatgaa cgcgcgcgtg cagagctgaa tcaggcaaat gaagatgttg ccagaaatca
     2821 ggagcgacag gctaaagctg ttcaggttta taattcgcgt aaaagcgaac ttgatgcagc
     2881 gaataaaact cttgctgatg caatagctga aataaaacga tttaatcgat ttgcccatga
     2941 cccaatggct ggcggtcaca gaatgtggca aatggccggg cttaaagctc agcgggcgca
     3001 gacggatgta aataataagc aggctgcatt tgatgctgct gcaaaagaga agtcagatgc
     3061 tgatgctgca ttaagtgccg cgcaggagcg ccgcaaacag aaggaaaata aagaaaagga
     3121 cgctaaggat aaattagata aggagagtaa acggaataag ccagggaagg cgacaggtaa
     3181 aggtaaacca gttggtgata aatggctgga tgatgcaggt aaagattcag gagcgccaat
     3241 tccagatcgc attgctgata agttgcgtga taaagaattt aaaaacttcg acgattttcg
     3301 gagaaaattc tgggaagaag tgtcgaaaga tcctgagtta agtaagcaat ttaatccagg
     3361 taataaaaaa cgcttgagcc aaggattagc tccacgggct aggaataaag atactgtcgg
     3421 tgggcgtcgc tcatttgagc ttcatcacga caagccaatc agtcaggatg gtggtgttta
     3481 tgatatggac aacctccgta tcactacgcc gaaacgccat attgatattc acagaggtca
     3541 gtaaaaatgg aactgaaaaa cagcattagt gattacactg aaactgaatt caaaaaaatt
     3601 attgaagaca tcatcaattg tgaaggtgat gaaaaaaaac aggatgataa cctcgagcat
     3661 tttataagtg ttactgagca tcctagtggt tctgatctga tttattaccc agaaggtaat
     3721 aatgatggta gccctgaagc tgttattaaa gagattaaag aatggcgagc tgctaacggt
     3781 aagtcaggat ttaaacaggg ctgaaatatg aatgccagtt gtttaaggat gaatgactgg
     3841 cattctttca caacaaggag tcgttatgaa aaaaataaca gggattattt tattgcttct
     3901 tgcagtcatt attctggctg catgtcaggc aaactatatc cgggatgttc agggcgggac
     3961 cgtatctccg tcatcaacag ctgaagtgac cggattagca acgcagtaac ccgaaatcct
     4021 ctttgacaaa aacaaagcgt gtcaggctga ttctgatgcg cttttttttg aaatgtcaca
     4081 aaagttccat gtgggagatg ggatctaaaa tccctgtgca gaaccttcca tccggtggga
     4141 gaaaacttgt cattttgacc tgttcgccat tcggaacggt cgaaaccgat cgcgcatcgc
     4201 tttcgtgcat agttatgcag cgccctaaaa acgattctga cgcatttttc tggttcagcc
     4261 tggtgtttcc ttgtcttttt gcgttttttg cgtcagaacg cgtctgagag cgttttaagg
     4321 ggtacgtaca acaggagtta tggtaaatgg atcgggtttt cgggaaggtt cgacaggatt
     4381 tgccgttggg tgtagtgtaa gcgactgaaa aacaaacgcc ccgtaaatcg tgctctcacc
     4441 gccaagattg atcacgaaat tacagggcgc cgggttccgc gtttcccgat gggaaagcgc
     4501 ggttagttaa actgtgtacc gagagaaatc gtatcacatg agcgccgtac ttcaacgctt
     4561 cagggaaaaa ttaccgcaca aaccgtactg tacgaacgat ttcgcgtacg gcgttcgcat
     4621 tctgccgaaa aacattgcca ttcttgcccg tttcatccag cagaaccagc cacatgcact
     4681 gtactggctt ccctttgacg tggaccggac gggggcatca atcgactgga gcgaccggaa
     4741 ttgtccggcc ccgaacatca ccgtaaaaaa tccccgtaac gggcacgcgc atctgctcta
     4801 cgcgctcgcc cttcctgtga gaactgcgcc ggatgcatcg gcttcggcgc tcagatacgc
     4861 tgccgctatt gagcgtgcgt tgtgtgaaaa actgggcgcg gatgtgaatt acagcggcct
     4921 gatctgcaaa aatccgtgcc accctgaatg gcaggaagtg gaatggcgcg aggaacccta
     4981 cactctcgac gaactggctg attatctcga tttgagcgcc tcagcgcgcc gtagcgtcga
     5041 taaaaattac gggctggggc gaaactacca tctgttcgaa aaggtccgta aatgggccta
     5101 cagagcgatt cgtcagggct ggcctgtatt ctcacaatgg cttgatgccg tgatccagcg
     5161 tgtcgaaatg tacaacgcat cgcttcccgt tccgctttct ccggctgaat gtcgggctat
     5221 tggcaagagc attgcgaaat atacacacag gaaattctca ccagagggat tttccgctgt
     5281 acaggccgct cgaggtcgca agggcggaac taaatctaag cgcgcagcag ttcctacatc
     5341 agcacgttcg ctgaagccgt gggaggcatt agggatcagt cgagcgacgt actaccgaaa
     5401 attaaaatgt gatccagatc tcgcaaaatg agaccagata agccttatca gataacagcg
     5461 cccttttggc gtctttttga gcagtgatct ttcttgcgat ttcctccgtt ttgccccgta
     5521 atggcgtggc tttccgcatg attgaggtgg tagcgctcgc cgcagtctca tgaccgagcg
     5581 tagcgagcga atgagcgagg aagcgcaaag gcgtccggtg gtgcatgggc acttacgcgc
     5641 cggggcttag tggttctgcg gtttcgccgg tggtctgggt agcttctcca gctcgttaat
     5701 cagcggttgt agtcggttca catccgcctg tcttgtgact tcctttcgca gaaactggag
     5761 caggaacgca cgcagttgcg cttcttccgg cctccgtacc ctcgccagca tggcagcccc
     5821 cacaatgact ttttgcgccg tgtccaggct ccggctcttc gccttcaggc gctgtaatct
     5881 ggcctcagct tcggcaatct tctgttcgag tgttctgctc atttcgtgac tccgtgcgcg
     5941 gtgaaaaatc gtattttagc gcgtcactgg tagttgcaaa actgaactgg cataatgcac
     6001 gacacatcac gaagtgcgca cttatacaat cttcacttcg tttcgattgt gtgcgccctg
     6061 cggggcttaa agaaaacggc aaaaaggcat tacggaagaa atggcgattt atcatctaag
     6121 catgaaaatc atttcgcgaa aaaacggcta cagtgccgtt gcttctgctg cctaccgttc
     6181 cggctctgtc atacccgatg atcgtaccgg attaacccac gattacaccc gtaaacgcgg
     6241 cgttgatgat gcggtcattc tcacccctgt gaatgcaccg tcctggtgtg gtgaccgttc
     6301 cgttctctgg aatgcggtcg agaaagccga acagcgccgg aactcccagc tggcaaggga
     6361 gattgaactc gccattcccc gtgagatttc ccgcgaggcc gcacgggagg ccgttctcgc
     6421 ttttgtccgg gaaaactttg tcagtcgggg catgattgcc gatgtggcgt tccatcatat
     6481 ggaccggacc aacccccatg cacacatcat gctgaccacg agagctgtcg gggaaacggg
     6541 attcgcagga aaggtcaggg actggaacga ccgggcactc gccgagacgt ggcgcgcatc
     6601 atgggctgac catgcgaaca gagcgcttgc gaacgccggc taccaggaag agatagacca
     6661 tcgttcatac gagcgtcagg gactggagaa agcgccgggc cttcacctcg gaaaggctgc
     6721 ctgtgcgatg gaaaaacggg gcatggaaac agaacg
//
