LOCUS       NZ_CP135675             8910 bp    DNA     circular CON 07-OCT-2023
DEFINITION  Escherichia coli strain TUM9803 plasmid pMTY9803_untypable2,
            complete sequence.
ACCESSION   NZ_CP135675
VERSION     NZ_CP135675.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN35769040
            Assembly: GCF_032382315.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8910)
  AUTHORS   Komori,K., Aoki,K., Ishii,Y. and Tateda,K.
  TITLE     Analysis of the Plasmid-Driven Acquisition of blaCTX-M-14 in
            Escherichia coli ST131 Subclone H30
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8910)
  AUTHORS   Komori,K., Aoki,K., Ishii,Y. and Tateda,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2023) Department of Microbiology and Infectious
            Diseases, Toho University, 5-21-16, Omori-Nishi, Ota-Ku, Tokyo
            143-8540, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 13-SEP-2021
            Assembly Method                   :: SPAdes v. v3.15.5
            Assembly Name                     :: Toho-u_TUM9803_Comp_v1.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: missing
            Genome Coverage                   :: 179x
            Sequencing Technology             :: Complete Genomics
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/07/2023 15:02:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,120
            CDSs (total)                      :: 6,000
            Genes (coding)                    :: 5,606
            CDSs (with protein)               :: 5,606
            Genes (RNA)                       :: 120
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 394
            CDSs (without protein)            :: 394
            Pseudo Genes (ambiguous residues) :: 0 of 394
            Pseudo Genes (frameshifted)       :: 148 of 394
            Pseudo Genes (incomplete)         :: 272 of 394
            Pseudo Genes (internal stop)      :: 84 of 394
            Pseudo Genes (multiple problems)  :: 93 of 394
            Pseudo Genes (short protein)      :: 4 of 394
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP135675.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8910
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="TUM9803"
                     /db_xref="taxon:562"
                     /plasmid="pMTY9803_untypable2"
                     /geo_loc_name="Japan"
                     /collection_date="2010"
                     /collected_by="Toho University School of Medicine"
     gene            join(8846..8910,1..>112)
                     /locus_tag="RS166_RS30460"
                     /old_locus_tag="RS166_30460"
                     /pseudo=""
     CDS             join(8846..8910,1..>112)
                     /locus_tag="RS166_RS30460"
                     /old_locus_tag="RS166_30460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611469.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /name="transposase"
     gene            complement(1992..2225)
                     /locus_tag="RS166_RS30465"
                     /old_locus_tag="RS166_30465"
     CDS             complement(1992..2225)
                     /locus_tag="RS166_RS30465"
                     /old_locus_tag="RS166_30465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_029400396.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTEHLSQLYSESRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2232..2750)
                     /locus_tag="RS166_RS30470"
                     /old_locus_tag="RS166_30470"
     CDS             complement(2232..2750)
                     /locus_tag="RS166_RS30470"
                     /old_locus_tag="RS166_30470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_029400393.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKVLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMRQHRKDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEDIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<2788..3486)
                     /locus_tag="RS166_RS30475"
                     /old_locus_tag="RS166_30475"
                     /pseudo=""
     CDS             complement(<2788..3486)
                     /locus_tag="RS166_RS30475"
                     /old_locus_tag="RS166_30475"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3428..3751)
                     /locus_tag="RS166_RS30480"
                     /old_locus_tag="RS166_30480"
     CDS             complement(3428..3751)
                     /locus_tag="RS166_RS30480"
                     /old_locus_tag="RS166_30480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3816..4007
                     /locus_tag="RS166_RS30485"
                     /old_locus_tag="RS166_30485"
     CDS             3816..4007
                     /locus_tag="RS166_RS30485"
                     /old_locus_tag="RS166_30485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165974.1"
                     /translation="MTKQEKTALNMARFIRGQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            4677..4835
                     /locus_tag="RS166_RS30490"
                     /old_locus_tag="RS166_30490"
     CDS             4677..4835
                     /locus_tag="RS166_RS30490"
                     /old_locus_tag="RS166_30490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001354639.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDKAPLAAAVGNLVSRAMMA
                     QMVQQSS"
                     /name="hypothetical protein"
     gene            complement(5032..5169)
                     /locus_tag="RS166_RS30495"
                     /old_locus_tag="RS166_30495"
     CDS             complement(5032..5169)
                     /locus_tag="RS166_RS30495"
                     /old_locus_tag="RS166_30495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001519492.1"
                     /translation="MRKRFFVGILAINLLVGCQANYIRDVQGGTVAPSSSSKLTGISVQ
                     "
                     /name="colicin release lysis protein"
     gene            5331..5558
                     /locus_tag="RS166_RS30500"
                     /old_locus_tag="RS166_30500"
     CDS             5331..5558
                     /locus_tag="RS166_RS30500"
                     /old_locus_tag="RS166_30500"
                     /inference="COORDINATES: protein motif:HMM:NF015489.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="WP_015056439.1"
                     /translation="MLYAIVISTILCPYSKYAIEHIFFKFIKKDFFRKRKNLNNAPVAK
                     LNLFMLYNLLCLVLAIPFGLLGLFISIKNN"
                     /name="colicin E1 immunity protein"
     gene            complement(5555..7120)
                     /locus_tag="RS166_RS30505"
                     /old_locus_tag="RS166_30505"
     CDS             complement(5555..7120)
                     /locus_tag="RS166_RS30505"
                     /old_locus_tag="RS166_30505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0140911 - pore-forming activity [Evidence
                     IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA];
                     GO:0050829 - defense response to Gram-negative bacterium
                     [Evidence IEA]; GO:0031640 - killing of cells of another
                     organism [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /protein_id="WP_000447001.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGNPDGSGSGGGGGTGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQHLKDIVNEALRH
                     NSTHPEVIDLAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIEKEQ
                     AETERQLKLAEAEEKRLAALNEEARAVEVAQKNLAAAQSELAKVDEEIKTLNTRLSSSI
                     HARDAEMNTLSGKRNELAQASAKYKELDELVKKLSPRANDPLQSRPFFYATSRRTGAGK
                     IMEEKQKQVTASETRINQLNAEINGIQGAMSQANNNRNTAVLRVHESEENLKTAQTNLL
                     NSQIKDAVDATVSFYQTLSEKYGEKYSKMAQELADKSKGKKISNVNEALAAFEKYKDVL
                     NKKFSKADRDAIFNALESVKYEDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTGD
                     WKPLFLTLEKKTVDAGVSYIVVLLFSVLAGTTLGIWGIAIVTGILCAFIDKNKLNTIND
                     VLGI"
                     /name="colicin-like pore-forming protein"
     gene            7398..7817
                     /locus_tag="RS166_RS30510"
                     /old_locus_tag="RS166_30510"
     CDS             7398..7817
                     /locus_tag="RS166_RS30510"
                     /old_locus_tag="RS166_30510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000312320.1"
                     /translation="MAWHTLQNALKMTGRSRSQFYRDMRAGLVSYRTGQDGRREFETSE
                     LIRAYGELKQDETPERHSAGQAENPHDQQTERILRELNELRQCLTLMLEDKQAQATERR
                     RQEAEREQLHKEIEQLRQALEQEKKRGFWARLFSR"
                     /name="hypothetical protein"
     gene            7901..8320
                     /locus_tag="RS166_RS30515"
                     /old_locus_tag="RS166_30515"
     CDS             7901..8320
                     /locus_tag="RS166_RS30515"
                     /old_locus_tag="RS166_30515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254598.1"
                     /translation="MRRSGGTLYLIALLTAATVLTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLAETISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     Typing          complement(4093..4180)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.753"
                     /coverage="100.0"
                     /evalue="1.48e-35"
     Typing          4203..4749
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.841"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(1889..3438)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.656"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ccagggcgaa tatgactcac aatgggcggc aatttgttcc attgctccaa agattggctg
       61 tacgccagag accctgcgtg tctgggttcg ccagcatgag cgggataccg ggtattacct
      121 ttcgaagaaa tttttgacat cctctacgcc ctgaaggacc gggttttacg gcgtaccaga
      181 taaaaaaacc cctctactga agaggggtag tgtaaggaac ttgctgattt tcttgatatt
      241 attaaccatc aataactgag catacaaaaa ataacaaaac tctgacacag atacaaccat
      301 ccctctttgt ataagaccga tgtgcatctc aaaaaagaag cgtgtttctg gaacacagaa
      361 caacaatgca cattgtagcc aacaaattca caccagaaac acgctctgtt ttttaattta
      421 cacaagaata tatgtctgga aaatcaggaa gaagaaatta atgggcattc tgtatacgaa
      481 gggagagcgc gtttccggtg ccccatatta gatattatcc ctgcaacaaa caccagagac
      541 gcgctcataa ttcttctagc gtaaatgatc ctttcaggca agaaaaaagc cgtgattctg
      601 tatcacggcc ttaattttat cgaaaacacg gattttagta tgcacatgac tgtactcctg
      661 tggagtacaa ggaaatcata atcagcgacc aaaccattta tcaacagaaa accaacatat
      721 tgatgttatt tttgtgacaa atgttacaaa ttcgaaacca ctgtattttt actgcaacaa
      781 aatctggttg atctacaagt tacactgggg taaaaagttg tccggaatgt ttctgcgtag
      841 taaagcctct agccggattg cctttgcaaa aacgcaggaa cgttcccccc ttcatcagta
      901 ctcctgacgc tgtaaggagc tgactgatgc agacagggct gatgatatct aataaaagct
      961 atctcatttt tgcgcgtaca taaaataaat caggcacttc cctacactcc ccctccggaa
     1021 ttgagggggt tttctttaca atgtaaataa attgcttgcg taaagttcaa aaaaactgca
     1081 aatttttact gtagtgtcga gcttcatgta gctcaccagg gcatggacta aaaagaagta
     1141 aacccatctt ttaccccccc ttatacgggg ggttctttat agcacaaaga acacttataa
     1201 tttatcatct cacagctata aatataaaag aataatattc taaactacag ttatttatgc
     1261 agaacataac atcaaactca caataaatat aaaacaatta ttttataact gttcacttat
     1321 tatattcaga catatcagaa acacgactca attctttttt atttaacaaa atccataaaa
     1381 aacttatggt tgaaatcaca tttaaactgt gaaggatatc atccggagaa atttttcaat
     1441 ccgacactcc atagaatgga cctttaagac tttatcctca tcatgtatga ggattttttt
     1501 tctggcaaac agtatctttc agtaatcctt gctcagttcc gattctgacg tatccccgtg
     1561 tggatctgaa aaggagataa ttacgctctg tggccaacag gaataactca agcagctcct
     1621 ctctgatacc atcagaagaa gcattaaacg cattctggcg tcgattacag aagaagcgga
     1681 acgcgttcca ggctcctgtt gctatacaca ctacccacac taaccccatg gtttttgagc
     1741 gccagaaacg cgtccgtatg cgttttaacg cgttcctgtg ttttcagcaa ggcctgactg
     1801 aagctagttc ctagtgccct gaaccaacca ttatccaaaa aaatgcaccg gaaacgcgct
     1861 gacaacactt tagcctgtct ttggttacca tcccagcgac gggccatcca gtgtccgttc
     1921 ctgtatttcc agatggcgct cacgctctag gaatagctca tgttcacgtt cctgtatcag
     1981 tagttcgtcc cttatctcct gctctcgctg tataactggc tcaagtgttc tgtctgctcg
     2041 ctcaagtgct gcacctgctg actcaactgc atgacccgct cgttcagcat cgcgttgtcc
     2101 cgttgcgtaa gcgaaaacat cttctgcaat tccacgaagg cgctctccca ttcgttcagc
     2161 cgctgcatat agtcctgttg cagctgctct aaggcgttca gcaaatgtgt ttccagctct
     2221 gtcactctgt gtcactcctt cagatgtacc cactctttcc cctgaaaggg aatcacctct
     2281 gctgatttcc cgtacggaag gaccagaaat ttcctgttcc cgtcctgcac gaactccacg
     2341 ccccatgtct tcgcgttcag tttctgcaat gtctcttcct gcttcctgat atcttccagg
     2401 ttcgcctgta tcctcccgcc aagataccag agcgtcccgc cactcgcggt aaacaggaga
     2461 aagactatcc ccagtaacat catgcccgta ttccctgcca gctttaacac gtccttcctg
     2521 tgctgcctca tcgcctcttt caccccttcc cggtgtttct ccagcgattc ctctgtcgag
     2581 gctgtgaaca gggctatagc gtctctgatt ttcgtctcgt ttgatgtcac agcctcgctt
     2641 acagattcgc cgagcctcct gaactcgttg ttcagcactt tctctgtaga ttcggctctc
     2701 tctttcagct ttttctcgaa ctccgcgccc gtctgtaaaa gattgctcat aaaacgctcc
     2761 tttcagcctg atattcttcc cgccgttcgg gtctgcaatg ctgatgctgc ttcgcgtcac
     2821 cctgaccact tcaagccccg cctctgtgag cgcctgaatc acatcctgac ggccttttat
     2881 ctcccctaca tgataaagag cgtctatccc gcgtgtgacg ctctcagcaa gcgcctgttt
     2941 cgtttccggc aggttatcag agagagtcag tatccggcgg ttctccgggg cgttggggtc
     3001 atgcagcccg taatggtgat tcaccagcgt ctgccaggca tcaattctcg gcctgtctgc
     3061 ccggtcgtag tacggctgga gacgttttcc ggtctgtagc tccatgttcg gaatgacaaa
     3121 attcagctca agccgtccct tgtcctggtg ctccacccac aggatgctgt actgattctt
     3181 ttcgagacct ggcatcagta cgcgctcaaa gctcgccatc actttttcac gtcctcccgg
     3241 cggcagctcc ttctccgcga acgacagaac gcctgacgtg tatttcttcg caaatggcgt
     3301 ggcatcgatg agttcccgga cttcttccgg attaccctga agcaccgttg cgccttcgcg
     3361 gttacgctcc ctccccagca ggtaatcaac cggaccactg ccaccacctt ttcccctggc
     3421 atgaaattta actatcatcc cgcgccccct gttccctgac ggccagacgc agccggcgca
     3481 gctcatcccc gatggccatc agtgcggcca ccacctgaac ccgatcaccg gaagaccact
     3541 gcccgctgtt caccttcctc gcggtctgat tcaggttatt cccgatggcg gccagctgac
     3601 gcagtaacgg cggtgccagt gtcggcagct tcccggaacg ggcaaccggc tcccccaggc
     3661 agacccgccg catccatacc gccagttgtt taccctcaca gcgttccagt aaccgggcat
     3721 gttcatcatc agtaacccgt atcgtgagca tcctctcgcg tttcatcggt atcattaccc
     3781 ccatgaacag aaatccccct tacacggagg catcagtgac taaacaggaa aaaaccgccc
     3841 ttaacatggc ccgcttcatc agaggccaga cattaacgct gctggagaaa ctcaacgaac
     3901 tggacgcgga tgaacaggca gatatctgtg aatcgcttca cgaccacgcc gatgagcttt
     3961 accgcagctg cctcgcacgt ttcggggatg acggtgaaaa cctctgacac atgcagctcc
     4021 cggagacggt cacagcttgt ctgtgagcgg atgccgggag ccgacaagcc cgtcagggcg
     4081 cgtcagcagg ttttagcggg tgtcggggcg cagccctgac ccagtcacgt agcgatagcg
     4141 gagtgtatac tggcttagtc atgcggcatc ggtgcggatt gtatgaaaag tgcaccatgt
     4201 acggtgtgaa atgccgcaca gatgcgtaag gagaacatgc agatgccgat gctcttccgc
     4261 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg tggcgagcgg tgtctgctca
     4321 ctcaaaagcg gtgatactgt tatccacaca atcaggggat aacgccggaa agaacatgtg
     4381 agcaaaaaac gaagacccca gaaaaggccg cgccggaggc gctttttcca taggctccgc
     4441 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     4501 cttaaagata ccaggcgttt ccccccggaa gctccctcgt gcgctctcct gttccgaccc
     4561 tgccgcttac cggatacctc tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     4621 gctcacgctg ttggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     4681 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     4741 acccggtaag acacgacaaa gcgccactgg cagcagccgt tggtaacttg gttagcagag
     4801 cgatgatggc gcaaatggtg caacagagtt cttgaagtag tggcccgact acggctacac
     4861 tggaaggaca gtatttggta tctgcgctct gctgaagcca gttacctcgg ttaagcagtt
     4921 ccccaactga cttaaccttc gatcaaaccg cctccccagg cggttttttc gtttacaggc
     4981 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctactgaac
     5041 agagatcccc gtcagtttag aagaagatga tggagccaca gtccctccct gaacatcacg
     5101 tatatagtta gcctgacatc caacaaggag gtttatagct aatattccca caaaaaatct
     5161 tttcctcata actcgatcct tataaaatga aaagaatata taacgaggtt taatttatga
     5221 gtttaagata ctacataaaa aacattttgt ttggcctata ttgcacactt atatatatat
     5281 accttataac aaaaaacaac gaagggtatt atttcctagc gtcagataag atgctatacg
     5341 caatcgtgat aagcactatt ctatgcccat attcaaaata tgctattgaa cacatatttt
     5401 ttaagttcat aaagaaagat tttttcagaa aaagaaaaaa tctaaataat gcccccgtgg
     5461 caaaattaaa cctatttatg ctatacaatc tactttgttt ggtcctagca atcccatttg
     5521 gattgctagg actgtttata tcaataaaga ataattaaat acccaacaca tcatttatag
     5581 tattaagttt attcttgtca ataaaggcac atagaatgcc tgtaacaata gcaatccccc
     5641 agatacctaa tgtagttcca gcaagcacac taaaaagtaa aacaacaata taactaactc
     5701 cagcatctac agttttcttc tctaatgtaa gaaatagtgg cttccagtca cctgtatcct
     5761 taatttttag gatatcagat accacatcat atccaaaaga aacatgtccc gtaatcttca
     5821 agtacttagc aaactgatct aaatgcttag cccagtcttc atacttaacc gattccagtg
     5881 cattgaaaat cgcatcacgg tctgctttgc tgaatttctt atttaaaaca tccttgtatt
     5941 tttcaaaagc agctagagct tcattcacat tgctgatttt cttaccttta gacttatcag
     6001 caagttcctg tgccattttt gaatattttt caccatattt ttcagatagc gtttgataaa
     6061 agctaactgt tgcatccaca gcatccttaa tctgcgagtt caggagatta gtctgcgctg
     6121 ttttcaaatt ttcttcagat tcatgaacac gtaaaacagc tgtattacga ttattattag
     6181 cctgagacat agccccctga attccattta tctcagcatt aagttggtta atacgcgttt
     6241 ctgatgctgt tacctgtttt tgtttttcct ccataatctt accggctccc gtccgtcgac
     6301 tggtggcata gaaaaaagga cgactctgaa gcggatcatt agctcttggt gacaattttt
     6361 tgaccagttc atcaagttcc ttatatttag cagatgcctg agccagctca tttcgttttc
     6421 cggacagcgt attcatttct gcatcacggg catgaatact ggagcttaaa cgggtattga
     6481 gagtcttaat ctcttcatcc actttcgcca gctcagattg tgcagcagca agattttttt
     6541 gtgccacctc cactgcccgg gcctcttcat tcaatgctgc cagccgtttc tcttcagctt
     6601 cagccagttt caactggcgt tctgtttcag cctgctcctt ctctatctct ttacgtcgtt
     6661 gttctgcctc ctgaaaagcc ttttccgcag cttccgcttc tttacgggct ttttcttctg
     6721 cttttgcaag gcgcaaccgc tctgcttctg cctgcatcgc tgcattattg gcatgagcca
     6781 ggtcaataac ctccggatga gtggaattat gacgaagcgc ctcattcaca atatccttca
     6841 gatgttgagt cagcgcatcc cggtttgctt ttgctttagc ctgtgcttct gcggcagctt
     6901 ttgcccgggc agcctgttct gcctgcgttt tcttcaattg agcagtagac catttagctg
     6961 tggcatgaat ggctgcagaa ctttcacttt tgctacctcc agttccacca ccgccgccag
     7021 agccactccc gtctggatta ccattcaaaa gagtaatgat tacctgcccc ttatcatcat
     7081 aaggaacacc atctttatag tacgctacag ctgtttccat tataaaatcc tctttgacat
     7141 taaaaacaaa tagttaaaag caatatactg tatatataac cactggtttt atatacagct
     7201 taaaattacg ccgctgcatt ttccctgtca accctgtgga ttttcatttt tgtgaaaacg
     7261 atcaaaaaaa cagccaccca caccacgtgc tattcccacc cgatgccaca aaaaccagca
     7321 caaacattac cgttctccgg ccccagtatg ttttactgaa accatgatat gagacattaa
     7381 tgggacactg tcacttcatg gcatggcaca cactccagaa cgcactgaaa atgacaggca
     7441 gatcgcgttc acagttttac cgtgatatgc gcgcaggcct tgtcagttat cgtacaggcc
     7501 aggacggacg gcgggagttt gaaaccagtg aactgatccg ggcatacggc gaattaaagc
     7561 aggatgagac accagaaagg cacagtgcgg gacaggcaga aaatccacat gatcagcaga
     7621 cagaacgcat tctccgggag ctgaatgagc tgagacaatg cctgacactg atgcttgagg
     7681 acaaacaggc acaggctacg gaacgccggc gccaggaagc cgaacgggaa cagctacata
     7741 aagagataga gcagctcagg caggcactgg agcaggaaaa aaaacgggga ttctgggcca
     7801 gactgttcag ccggtgagca ccgactgcaa gtgatgataa gatagtcagc ggttaaatat
     7861 aagtcactga gtacaaagat ttaaaggtaa ggagacactc atgagacgtt ccggcggcac
     7921 actgtatctt atcgccctgc tgacagcggc aacggtcctg acagcctgca cgccaaaggg
     7981 cagtgtggaa caacataccc ggcattacgt ttatgcatca gacgatggtt ttgatcctaa
     8041 cttttccacc cagaaggccg acacaacacg aatgatggtg ccttttttcc ggcagttctg
     8101 ggatatggga gcaaaggata aagcgacagg aaaatcacgg agtgatgtgc agcaacgcat
     8161 tcagcagttt cacagccagg aatttttaaa ctcactccgg ggcacaactc aatttgcggg
     8221 tactgattac cgcagcaaag accttacccc gaaaaaatcc aggctgctgg ctgagacgat
     8281 ttctgcggtt tatctcgatg gctacgaggg aagacagtaa gtggatttac cataatccct
     8341 taattgtacg caccgctgaa atgcattcag cgcaatcacg gcagcagaca ggtaaaaatc
     8401 gcaacaaccc ccccgaaaaa ctaccgcgat cgcgcctgat aaattttaac cttatgcata
     8461 cctatgcatc caggtgaatc acaagcgaat ttccttactg agacgactga aacggttttt
     8521 tttcacaatt ttcagctgtt ttcgattcca caaaatagaa gttacaggat agggattaat
     8581 ccccttcgcc gccaagggga gcaggatgag tcaggggaat gtctattcac ctgcgctgta
     8641 caggctgcca ggtaaaagta ttcgctgcgg cacgatactc ttttgccctc atagcctttg
     8701 cgcttcttac ttttcgttct tccagaatca ttcttaagac attttcccgc acttttctct
     8761 aacttcccgt ggcaatttat ctctgccgga ctgaaccgcc ccggaaatcc tggagactaa
     8821 acttcctgag aaagatgtaa acaggatgac taaaaatact cgtttttccc ccgaagtccg
     8881 tcaacgggca gttcgtatgg ttctggaaag
//
