LOCUS       NZ_CP135663             2089 bp    DNA     circular CON 07-OCT-2023
DEFINITION  Escherichia coli strain TUM10008 plasmid pMTY10008_Col2, complete
            sequence.
ACCESSION   NZ_CP135663
VERSION     NZ_CP135663.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN35769064
            Assembly: GCF_032375225.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Komori,K., Aoki,K., Ishii,Y. and Tateda,K.
  TITLE     Analysis of the Plasmid-Driven Acquisition of blaCTX-M-14 in
            Escherichia coli ST131 Subclone H30
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Komori,K., Aoki,K., Ishii,Y. and Tateda,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2023) Department of Microbiology and Infectious
            Diseases, Toho University, 5-21-16, Omori-Nishi, Ota-Ku, Tokyo
            143-8540, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 13-SEP-2021
            Assembly Method                   :: SPAdes v. v3.15.5
            Assembly Name                     :: Toho-u_TUM10008_Comp_v1.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: missing
            Genome Coverage                   :: 196x
            Sequencing Technology             :: Complete Genomics
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/07/2023 14:08:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,263
            CDSs (total)                      :: 5,143
            Genes (coding)                    :: 4,853
            CDSs (with protein)               :: 4,853
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 290
            CDSs (without protein)            :: 290
            Pseudo Genes (ambiguous residues) :: 0 of 290
            Pseudo Genes (frameshifted)       :: 72 of 290
            Pseudo Genes (incomplete)         :: 213 of 290
            Pseudo Genes (internal stop)      :: 50 of 290
            Pseudo Genes (multiple problems)  :: 48 of 290
            Pseudo Genes (short protein)      :: 5 of 290
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP135663.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="TUM10008"
                     /db_xref="taxon:562"
                     /plasmid="pMTY10008_Col2"
                     /geo_loc_name="Japan"
                     /collection_date="2010"
                     /collected_by="Toho University School of Medicine"
     gene            782..1747
                     /locus_tag="RS168_RS26310"
                     /old_locus_tag="RS168_26310"
     CDS             782..1747
                     /locus_tag="RS168_RS26310"
                     /old_locus_tag="RS168_26310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001287824.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     gene            1751..2017
                     /locus_tag="RS168_RS26315"
                     /old_locus_tag="RS168_26315"
     CDS             1751..2017
                     /locus_tag="RS168_RS26315"
                     /old_locus_tag="RS168_26315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024536.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     Typing          1176..1407
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 tcctgcatta tcctcagcat tatcctcagc cttgccaact cgacaccaat gcaggataga
       61 caatccgatg tcaaatgtta acactctgcg agtggtacat tttccccgga ttatcgtcct
      121 gagcctgccg ctggctctct ttctaccgcc tcgctttgct cgttgctcaa cgcctcacag
      181 acacggatta aaatccgcat ccgttcaccg ttttttaaag tccgttaaaa gcatgatgcc
      241 atctccgaga gttaatctcg tcaaatgcta aatcgtgggg gtcccctttg gggttccgat
      301 ttagtgattg acgacaccac cgattaaaaa acttatgcgg ggtggatggt ttcacgaagt
      361 gaggccatcc acctgtaaga cagggttttg tttttattcc ctgttttggt gatcgggtgt
      421 gtggaaaagg ttggggtaag ccgttcgggg gtgcttgttt tggggggtta aaattgtggt
      481 tattttttgc gcaattctcg cgcgtgatcc ttgtatttat acttaaggga taaatggcgg
      541 atatgaaata gtggtttagc ccagtaatga cgaggctttg agtgggtttt gacaggtcaa
      601 agaaaatgga gcagaattga ggcgttttta atcggcgttg gggagtgcgt caacactccc
      661 caacatttcg aatgtgtcac ctcagcggca aactctggtg acatgtactg gctcgcaatg
      721 cacaggtacg tgatgaatat accacatcaa atcacagcct gcccagatcg gagcaggctt
      781 aatgtcagaa gataaattcc tttcggacta cagcccccgt gatgcagttt gggataccca
      841 gcgcacgctt accgattctg tcgggggtat ctaccagact gctgctgaat tcgagcgcta
      901 tgcactccgt atggcctcct gtagcggttt gttacgtttt ggttggtcta ccatcatgga
      961 aaccggagaa acgcgcctac ggcttcgtag tgcgcaattt tgccgtgtcc gtcattgccc
     1021 tgtctgccag tggagaagaa ccctcatgtg gcaagcccgt ttttatcagg ctctaccgaa
     1081 aatcgttgtg gattacccgt cttcccgatg gttgtttctg acgttaactg tcaggaactg
     1141 cgagataggt gaacttggaa cagtccttac agcaatgaat gcggcgttta agcgaatgga
     1201 aaagcgaaag gagctatcac ctgttcaggg gtggatcagg gctacggagg tgacgcgagg
     1261 taaggatggc agcgcacatc cgcattttca ctgtctgctg atggtgcaac cttcttggtt
     1321 taaagggaag aactacgtta agcacgaacg ttgggtagaa ctctggcgcg attgcttgcg
     1381 ggtgaactat gagccgaata tcgatattcg ggcagtaaaa actaagacag gtgaggttgt
     1441 ggccaacgtt gccgagcaac tgcaaagcgc ggttgctgaa acgctgaaat actccgttaa
     1501 accggaagat atggcaaacg atcctgagtg gtttcttgag ctgacgcggc agcttcacaa
     1561 gcgccgtttt atctcgaccg gtggggcgct aaaaaacgtc ctccagttgg atcgagaaac
     1621 caatgaggat cttgtcattg ccgacgatgt aggggatggc actgatgacg ggaagcggac
     1681 ggcgtttgtc tgggattcag gtaaacggcg ttacaaacgc gcccctgaga aggataaatc
     1741 ggattaacgt atgaatatta atattgaata cctgaatgga aataagacta ttggtttatt
     1801 ttttttaaga agtgaagcgg tgattcctga caggtttaaa aaccttattt tgcttattga
     1861 tggattaagt tttggcacat ttggttttca tccgcacgaa ggttttgagg atgaattaat
     1921 tttatatatt cagaaaacaa acgagagggt aaaaactctt tttgtgaaaa tagatttaaa
     1981 cggtatcaag tttggatttt taagaacgca ttcttagttc tggaaaagag ccagcggcag
     2041 gctgaggtga taggtacgag attgcatgca atctctagtg ctctgtcta
//
