LOCUS       NZ_CP135510             6141 bp    DNA     circular CON 07-OCT-2023
DEFINITION  Klebsiella pneumoniae strain isolateK plasmid pIsolateK_B, complete
            sequence.
ACCESSION   NZ_CP135510
VERSION     NZ_CP135510.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37529182
            Assembly: GCF_032343715.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 6141)
  AUTHORS   Lerminiaux,N., Fakharuddin,K., Mulvey,M.R. and Mataseje,L.
  TITLE     Do we still need Illumina sequencing data?: Evaluating Oxford
            Nanopore Technologies R10.4.1 flow cells and v14 library prep kits
            for Gram negative bacteria whole genome assemblies
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6141)
  AUTHORS   Lerminiaux,N., Fakharuddin,K., Mulvey,M.R. and Mataseje,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-SEP-2023) National Microbiology Lab, Public Health
            Agency of Canada, 1015 Arlington Street, Winnipeg R3E3R2, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 21-JUN-2023
            Assembly Method :: Trycycler v. v0.5.0; Flye v. v2.9.2; Raven v.
            v1.8.1; Miniasm/minipolish v. v0.3_r179
            Assembly Name                     :: isolateK
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 229X
            Sequencing Technology :: Oxford Nanopore MinION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/07/2023 13:32:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,641
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,398
            CDSs (with protein)               :: 5,398
            Genes (RNA)                       :: 126
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 42 of 117
            Pseudo Genes (incomplete)         :: 73 of 117
            Pseudo Genes (internal stop)      :: 20 of 117
            Pseudo Genes (multiple problems)  :: 15 of 117
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP135510.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6141
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="isolateK"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pIsolateK_B"
                     /geo_loc_name="Canada: Ottawa"
                     /collection_date="2020"
     gene            join(5814..6141,1..470)
                     /locus_tag="RRL08_RS28105"
                     /old_locus_tag="RRL08_28105"
     CDS             join(5814..6141,1..470)
                     /locus_tag="RRL08_RS28105"
                     /old_locus_tag="RRL08_28105"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011071128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-232"
                     /protein_id="WP_043907054.1"
                     /translation="MRVLALSAVFLVASIIGMPAVAKEWQENKSWNAHFTEHKSQGVVV
                     LWNENKQQGFTNNLKRANQAFLPASTFKIPNSLIALDLGVVKDEHQVFKWDGQTRDIAA
                     WNRDHDLITAMKYSVVPVYQEFARQIGEARMSKMLHAFDYGNEDISGNVDSFWLDGGIR
                     ISATQQIAFLRKLYHNKLHVSERSQRIVKQAMLTEANGDYIIRAKTGYSTSIEPKIGWW
                     VGWVELDDNVWFFAMNMDMPTSDGLGLRQAITKEVLKQEKIIP"
                     /name="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-232"
     gene            complement(877..>1029)
                     /locus_tag="RRL08_RS28110"
                     /old_locus_tag="RRL08_28110"
                     /pseudo=""
     CDS             complement(877..>1029)
                     /locus_tag="RRL08_RS28110"
                     /old_locus_tag="RRL08_28110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015059985.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="EreA family erythromycin esterase"
                     /name="EreA family erythromycin esterase"
     gene            1405..2352
                     /locus_tag="RRL08_RS28115"
                     /old_locus_tag="RRL08_28115"
     CDS             1405..2352
                     /locus_tag="RRL08_RS28115"
                     /old_locus_tag="RRL08_28115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014293673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_015060053.1"
                     /translation="MRKCAQLELFRERLPRKPYYSDELTTGLRIADVARALGARYIQPN
                     GPTHRHWIVFDVDHAAATLSWDDVGAPAPNITVTNKANGHAHLIYGLDTPIRTAPDGNA
                     APLRYAAAIEAALREKLGADMGYSGLICKNPLHEHWLVQVWEPRLYDLAWLSDYLDLSS
                     YNGRKSLPEYGLGRNCTLFEKTRLWAYKAIRQGWPDYPEWLAACVDRASGYNAQFEQPL
                     PANEVRHTAKSIAKWTHQHLSPAGFREEQARRGAKGGKVSKGGGRPSNSGKDKSDLLPE
                     VLRLKAQGYTNRDIADDLQISPSTVSVYLKRDRP"
                     /name="replication initiation protein"
     gene            2760..3152
                     /locus_tag="RRL08_RS28120"
                     /old_locus_tag="RRL08_28120"
     CDS             2760..3152
                     /locus_tag="RRL08_RS28120"
                     /old_locus_tag="RRL08_28120"
                     /inference="COORDINATES: protein motif:HMM:NF037437.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VbhA family protein"
                     /protein_id="WP_043907050.1"
                     /translation="MSTTQIEKDSRRKAVNGVISSFQLEGEAITDEFKILLEDYINGKI
                     STDEVLAMVSDEPTTTEYDYEITPRTLGLGGGWNLRLLENGEEVGGGVFPLPEHCDFRD
                     EKALQTLLDSLYEDALAEASAWLASR"
                     /name="antitoxin VbhA family protein"
     gene            3134..3472
                     /locus_tag="RRL08_RS28125"
                     /old_locus_tag="RRL08_28125"
     CDS             3134..3472
                     /locus_tag="RRL08_RS28125"
                     /old_locus_tag="RRL08_28125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_043907051.1"
                     /translation="MAGQPLKRLRSIKIRVSDAELERLRELCPKAQLAEWMREQCLGVA
                     QPQRRTPAPTVDPALLRQLAGMGNNLNQIARRVNSGEWGPLDRLRIIAELSAIGRELEE
                     LHHDHQIP"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3453..4907
                     /locus_tag="RRL08_RS28130"
                     /old_locus_tag="RRL08_28130"
     CDS             3453..4907
                     /locus_tag="RRL08_RS28130"
                     /old_locus_tag="RRL08_28130"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_064996073.1"
                     /translation="MIIKFHNRGVGRGSGPVGYLLGRDGNREGATLDRGNPAVIEALID
                     SSPYAKKYTSGVLSFAEADLPRATKDKLMSDFEKALLPGLEGNQYAVLWVEHRDKGRLE
                     LNFVVPNIELQSGKRLQPYFHKADNPRLDAWRTVVNGELKLHDPDDPLNRQAVITPKDL
                     PRGHKEAAQAITDGLLRMAGAGEIQSRDDVRKALQGAGFSVVRETKNSLSIADPDGGRN
                     IRLKGVLYEQDFRHGEGLRAELEAAGQRYREASDRRIHEARESYLRGVEIKRGENERRY
                     CRAQPENGLAVAQNVDLDAGQHRPVLERNERDPLVSGGIDSGAASHSPSLGREGRQAKA
                     DPVREEIGAPLRADLRPQSNRLGEGYLLDRAEGVLNDGIGAGIALRVRGFAERIRAAAQ
                     GMAGKLRELGEHVREHQTRGRDQQGSSRAIDEAGERLNAASGRLEQAAGGLDRASQQVA
                     KEREHRLLKALDRGWERGDRSRGFER"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(5189..5470)
                     /locus_tag="RRL08_RS28135"
                     /old_locus_tag="RRL08_28135"
     CDS             complement(5189..5470)
                     /locus_tag="RRL08_RS28135"
                     /old_locus_tag="RRL08_28135"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_043907053.1"
                     /translation="MGFFNLSKALEEVSDAFGAKETAIASAKLLGKGVVNLAHFAATTG
                     LDSVVKQTSNKVLNSESSTEEQREQAKKSAAWADARIAARNKAENTED"
                     /name="hypothetical protein"
     ARG             4..470
                     /name="OXA-232"
                     /gene="OXA-232"
                     /gene_name="OXA beta-lactamase; OXA-48-like beta-lactamase"
                     /tool="rgi"
                     /drug_class="carbapenem; penam"
                     /identity="100.0"
                     /coverage="58.49"
     ARG             5815..6140
                     /name="OXA-833"
                     /gene="OXA-833"
                     /gene_name="OXA beta-lactamase; OXA-48-like beta-lactamase"
                     /tool="rgi"
                     /drug_class="carbapenem; penam"
                     /identity="100.0"
                     /coverage="41.13"
     Typing          1418..2339
                     /name="rep_cluster_1195"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="81.974"
                     /coverage="98.0"
                     /evalue="0.0"
     Typing          3454..4904
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.554"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 acttaattac cgcgatgaag tactcagttg tgcctgttta tcaagaattt gcccgccaaa
       61 ttggtgaggc acgtatgagt aaaatgctgc acgccttcga ttatggcaat gaggatatct
      121 cgggcaatgt agacagtttt tggctcgatg gtggtattcg catttcggct acccagcaaa
      181 tcgctttttt acgcaagctg tatcacaaca agctgcacgt ttctgagcgt agtcagcgca
      241 tcgtgaaaca agccatgctg accgaagcca atggcgacta tattattcgg gctaaaacgg
      301 gatactcgac tagtatcgaa cctaagattg gctggtgggt tggttgggtt gaacttgatg
      361 ataatgtgtg gttttttgcg atgaatatgg atatgcccac atcggatggt ttagggctgc
      421 gccaagccat cacaaaagaa gtgctcaaac aggagaaaat tattccctag aagtggttag
      481 cgcgtatttg tgtgaaatag ccgtcatata agctgtaaag atatatggac aaaatgctga
      541 tgctcgatgc gcttgtagtt aatggctcat acttaatcgg tcaggcctaa tcgacttaag
      601 ctccgcgcgt taactttcag cctgagcatt agcatcacat tcctaaacgt aaaaagccta
      661 gccaatttgc taggcttttt tgttatctca atggccatgg tcactcaaca tgggggtgtg
      721 aataaaggag tggcgatgtc agtaaaggtg aatgccgctg cgataaagtg cctagcccat
      781 caaggattgt aggctttctt ggctgctaaa gcgtttgacg aaaaagtcca agaaggcgct
      841 gatattggtt ctccatcatt ttcatctacc ggtttactat aggtcaacca ggccgtcctt
      901 gccggcgctt gggacgcaga cgatggcatc aaatgcctcg ctcaaattcg tttctacaga
      961 ggcgctttgg gaccgcattc gctttgcttt catgggggca tctgtcaatg tcagacatga
     1021 atcctccgat ataggttgat ttttgatctc ttcaacactt ggtgttgtcc gactagagag
     1081 acttatatgg atcagtattc ttgcactgac aatggtgcca ctcaatccca aggatcgacc
     1141 tagttagagg cgtagatcaa gcaattcaaa acagcgcaga agcagaagga ggtggaaata
     1201 tgttccaggg tgaaaggtta catgcaacta acgttgctct ctaggatgtg cctgctgtaa
     1261 cgtattgtcc agaaaagaca accccccgtt atcgcttgca tcttggcgga gctgcgaaaa
     1321 acgggggccg tgacgaatca caagagaagt ctatcacatg gatcatgcca aacaacccca
     1381 cgccgccaat aagcgcggtg gtgcttgaga aagtgcgcac agcttgagct gtttcgggag
     1441 cgcctgcccc ggaaacccta ctattccgac gagcttacaa cgggcctacg gattgcggat
     1501 gttgcccgcg cccttggtgc gcgttacatc cagccgaacg ggccaacgca ccgccactgg
     1561 atcgtttttg acgtagacca tgcagccgct acgttatctt gggatgacgt aggcgctcca
     1621 gcccctaaca ttaccgtcac gaacaaggcc aacggccacg cgcatctgat ctacggactg
     1681 gatacgccga tccgcactgc cccggatgga aatgcagccc ctctgcggta tgccgccgcc
     1741 atcgaggccg cgctccggga gaaattaggg gcggatatgg gctactccgg gctgatctgc
     1801 aagaaccccc tgcatgagca ctggctggtg caggtatggg aaccccggct ctacgattta
     1861 gcctggctat ccgattacct cgatctgagc tcctacaatg gccgcaaatc gcttcctgag
     1921 tacgggcttg ggcgaaactg taccctgttc gagaaaacgc gcctgtgggc ctataaagcg
     1981 atccggcagg gctggccgga ttaccccgaa tggttggccg cttgtgttga ccgggcatca
     2041 ggatataacg cccagttcga gcagccgctc cccgcaaacg aagtccggca cacggccaag
     2101 tccattgcca aatggacgca ccagcaccta agccccgcag ggttcagaga ggagcaggcc
     2161 cggagaggtg cgaagggcgg caaggtatcc aagggagggg gaaggccgag caatagcggt
     2221 aaggacaagt ccgatctatt gccggaggtt ctgcggctta aggctcaggg gtacaccaac
     2281 cgcgatatag cggacgatct tcaaatctcc cccagtacgg tgagcgttta cctaaagcgt
     2341 gatcgtccgt gatcgaaaca agccttatca gataatagcc ccctttaggg gggttcctgc
     2401 gctcttgtct tttagcctta aaaaatcctt ttagcgcgga tctttggcga ggtggacaac
     2461 tgcgcctgtc cgtccatcgc ccccctgttt tttcgggggg tgtgggcggc agcagttatc
     2521 cacggagccg ctctttccct tgatcttgct ctagggcttg cagtatcccg cccatggttg
     2581 aataatgtac taagcaaggc tacacagagg cccctctacc tgcttcgcgg tctcgggcct
     2641 ctgtggcctt gttcaggggt gctacgcccc cccgaaaccc cctgtggccc ccgctcgatc
     2701 cgccccttcg ggacggtctg cgcggtggct tcttggctct ggagaatgga cgaggcccta
     2761 tgtcaacaac acagatagag aaagattcga gaaggaaggc agtcaatggc gttatttcta
     2821 gtttccaact agagggggag gccataaccg atgaattcaa aattctactt gaagattata
     2881 tcaatggaaa aatttccacc gatgaggtgt tagctatggt aagtgacgaa cccacaacaa
     2941 cggaatacga ctacgaaatc accccccgca ctctcggtct tggcgggggc tggaatctgc
     3001 gtttgctgga gaacggcgaa gaggtcggcg ggggtgtttt ccccttgccg gagcactgcg
     3061 attttcggga tgaaaaggcg ctccagacgc tactcgatag cctctatgag gacgccttag
     3121 cggaggcctc tgcatggctg gccagccgtt aaagcgcctc cgctctatca agatccgggt
     3181 ctcagatgcg gagcttgagc gcctgcggga gctctgcccc aaggcccaac tggcggagtg
     3241 gatgcgggag caatgtctcg gtgttgcgca accccagcgc cgaacgccgg cgcctaccgt
     3301 agatccggcg ctcttgcggc agttggccgg aatgggcaac aacctgaacc agatcgcccg
     3361 gcgcgttaat tccggggagt gggggccgtt ggatcggctg cgcatcatag cggagctgtc
     3421 cgctataggc cgcgagctgg aggaactgca ccatgatcat caaattccat aaccgtgggg
     3481 taggccgggg ctccgggcct gttggctact tgttgggccg tgacgggaac cgggaaggcg
     3541 caacgctcga ccggggcaac cctgcggtga ttgaagccct gattgattcc agtccctacg
     3601 ccaaaaaata cacctctggc gttctgtcgt ttgccgaggc tgatttaccc agggcgacca
     3661 aggacaagct gatgagcgac tttgaaaagg cgctcctgcc cggactggag ggcaaccagt
     3721 acgctgtttt gtgggtagag caccgcgaca aggggcggct ggagctgaat tttgtagtcc
     3781 cgaacatcga attgcagagc gggaagcggc tgcaacccta cttccacaag gccgacaatc
     3841 cgcgcctcga tgcgtggcga accgtggtaa acggggagct gaaattgcac gacccggacg
     3901 atccgttaaa ccgtcaggcc gttatcacgc caaaggattt gccgaggggg cacaaggagg
     3961 ccgctcaggc cattacagac gggcttttac gcatggccgg agcaggggag atccagagcc
     4021 gcgatgatgt gcgcaaagcc ttgcagggtg cgggtttcag cgtggtacgc gaaaccaaaa
     4081 acagcctgag cattgccgac ccggacgggg ggcggaacat ccgattaaag ggggtgctgt
     4141 atgagcaaga ttttagacat ggcgaaggac ttcgagcaga gctcgaagca gcaggccaac
     4201 gctatcgaga agcgtctgac cgaagaattc acgaggcacg agaaagctat ctccggggcg
     4261 ttgaaatcaa gcgaggagaa aatgagcgcc gctattgccg agcacagcca gaaaatgggc
     4321 tggctgttgc tcaaaacgtg gatctggatg ctggtcagca tcgtcctgtt cttgagcgca
     4381 acgagcggga tcctctggta tcagggggaa ttgatagcgg agcggcaagc cactctccaa
     4441 gccttggcag agaagggcgg caagctaaag ctgacccagt gcgggaagaa attggagcac
     4501 ctttgcgtgc agatctccga ccccaatcca accgtttggg ggaaggatac ctattggatc
     4561 gtgccgaagg ggtactaaat gacgggattg gagcgggaat tgctctgcgc gttcgaggct
     4621 ttgcagagcg catacgagca gcagcacaag gcatggcagg aaagctacgc gaacttggcg
     4681 agcatgtacg agagcaccaa acaagaggcc gagatcagca gggcagcagc agagcgattg
     4741 acgaggcagg tgaacggctt aacgcagcaa gtggacggct tgagcaagca gctggagggc
     4801 ttgaccgggc tagtcagcaa gttgcaaaag aaagggaaca caggctcctg aaggcactgg
     4861 atcgaggctg ggagcgtgga gacagatcgc gaggctttga gcgttgacgg aacaaaacgg
     4921 ccgtcagtta taaaaaaagg aaagtgctat cactttcctt ttatcggcgg gaattatcgt
     4981 tgagttcttc tatgccattt ttcatagtag taactgtaat cttcatctct accaccagat
     5041 tcaatccagt catcatactc agggctattt ttgaaatctg aatcagacat gctaactcct
     5101 tgttattagt tggcttctat ggaagctagc gcaccgtgaa gttatgatca acgatgtagt
     5161 ccacatcatt gtcgaaatgt gcgttcagct agtcttcggt gttttctgct ttatttctag
     5221 cggcgattct cgcgtctgcc catgcggcag actttttagc ctgttctcgt tgttcttcgg
     5281 ttgaagactc gctgttgaga actttgttgc tcgtttgttt gaccaccgaa tccaaacctg
     5341 ttgttgctgc aaaatgagct aggttaacaa ctcccttacc taacaacttg gcactagcga
     5401 ttgcggtttc tttagcccca aaagcatcag acacctcctc aagcgcttta gataggttaa
     5461 aaaatcccat actcccccta agaatatcat caataaaatt gagtgttgct ctgtggataa
     5521 cttgcagagt ttattaagta tcattgcagc aaagatgaaa tcaatgattt atcaaaaatg
     5581 attgaaaggt ggttgtaaat aatgttacaa tgtgtgagag gcagtctaaa ttcttcgtga
     5641 aatagtgatt tttgaagcta ataaaaaaca cacgtggaat ttaggtaata gttaaagctt
     5701 gtgcctgatt gttgccacaa ttggtcagca gtttgctagg gaatgagagg gcagagaatg
     5761 ataagcttag cacttaattt gtatggatat attgcattaa gcaaggggac gttatgcgtg
     5821 tattagcctt atcggctgtg tttttggtgg catcgattat cggaatgcca gcggtagcaa
     5881 aggaatggca agaaaacaaa agttggaatg ctcactttac tgaacataaa tcacagggcg
     5941 tagttgtgct ctggaatgag aataagcagc aaggatttac caataatctt aaacgggcga
     6001 accaagcatt tttacccgca tctaccttta aaattcccaa tagcttgatc gccctcgatt
     6061 tgggcgtggt taaggatgaa caccaagtct ttaagtggga tggacagacg cgtgatatcg
     6121 ccgcttggaa tcgtgaccat g
//
