LOCUS       NZ_CP135491            26248 bp    DNA     circular CON 07-OCT-2023
DEFINITION  Escherichia coli strain isolateD plasmid pIsolateD_C, complete
            sequence.
ACCESSION   NZ_CP135491
VERSION     NZ_CP135491.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37529175
            Assembly: GCF_032351055.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26248)
  AUTHORS   Lerminiaux,N., Fakharuddin,K., Mulvey,M.R. and Mataseje,L.
  TITLE     Do we still need Illumina sequencing data?: Evaluating Oxford
            Nanopore Technologies R10.4.1 flow cells and v14 library prep kits
            for Gram negative bacteria whole genome assemblies
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 26248)
  AUTHORS   Lerminiaux,N., Fakharuddin,K., Mulvey,M.R. and Mataseje,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-SEP-2023) National Microbiology Lab, Public Health
            Agency of Canada, 1015 Arlington Street, Winnipeg R3E3R2, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 21-JUN-2023
            Assembly Method :: Trycycler v. v0.5.0; Flye v. v2.9.2; Raven v.
            v1.8.1; Miniasm/minipolish v. v0.3_r179
            Assembly Name                     :: isolateD
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 245X
            Sequencing Technology :: Oxford Nanopore MinION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/07/2023 13:56:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,954
            CDSs (total)                      :: 4,833
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 275
            CDSs (without protein)            :: 275
            Pseudo Genes (ambiguous residues) :: 0 of 275
            Pseudo Genes (frameshifted)       :: 84 of 275
            Pseudo Genes (incomplete)         :: 187 of 275
            Pseudo Genes (internal stop)      :: 46 of 275
            Pseudo Genes (multiple problems)  :: 40 of 275
            Pseudo Genes (short protein)      :: 4 of 275
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP135491.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..26248
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="isolateD"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pIsolateD_C"
                     /geo_loc_name="Canada: Ottawa"
                     /collection_date="2020"
     gene            1..1032
                     /gene="repA"
                     /locus_tag="RRL15_RS24600"
                     /old_locus_tag="RRL15_24600"
     CDS             1..1032
                     /gene="repA"
                     /locus_tag="RRL15_RS24600"
                     /old_locus_tag="RRL15_24600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiator RepA"
                     /protein_id="WP_023518831.1"
                     /translation="MAGLKNTPYNAVHWSQLAPEEQIRFWEDYEAGRATTFLVEPERKR
                     TKRRRGEHSTKPKCENPSWYRPELYKALSGQLGHAYNRLVKKDPVTGEQSLRMHMSLHP
                     FYVQKRTYAGRKYAFRPEKQRLLDAIWPVLVSFSDAGTHTVGMSVSRLAREISPKDSKG
                     KVIPELEVTVSRLSRLLAEQVRFGVLGVSEETLWDRETRQRLPRYVWITPAGWQMLGVD
                     MVKLHEQQQKRLRESEIRQQLIREGVLREDEDISVHAARKRWYLQRSQDALKHRRAKAA
                     ASKRARRLKKLPADQQIHEMAEYLRKRLPPDEAYFCSDDHLKRLAIRELRQLELTLAAP
                     PPH"
                     /name="repA"
     gene            complement(2101..2610)
                     /locus_tag="RRL15_RS24605"
                     /old_locus_tag="RRL15_24605"
     CDS             complement(2101..2610)
                     /locus_tag="RRL15_RS24605"
                     /old_locus_tag="RRL15_24605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012783913.1"
                     /translation="MSKSGAEKIQFILNNHEQAYRRLKPVYTFLELASWISVVLLIIYL
                     SGKNHVLTAAFLCFAWTAFWVLFLLHYFPKVFSILDRQSLLNLMEATKDSPDVKQELLN
                     RLFSGKRMTGRDEMDICRLLRKNEEEKIRQSELNIIRNFIDEQQLTNSDIPKKSTVPNE
                     DKKGIR"
                     /name="hypothetical protein"
     gene            complement(2666..3268)
                     /locus_tag="RRL15_RS24610"
                     /old_locus_tag="RRL15_24610"
     CDS             complement(2666..3268)
                     /locus_tag="RRL15_RS24610"
                     /old_locus_tag="RRL15_24610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001703912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="WP_001703912.1"
                     /translation="MEKNQEERKIPVIVVKKRRTFSLPSLSEKTDIIESAFTEQAAESA
                     PVGINSSAVETHIPEAPARKKKKKKRRFPRPSHWTREYTHECVEKIKILFPHLRAEGGG
                     FIPLKIGINNDISAFLAEHPETELTMDEWLCAVSCITSRRVYLQRTAVAGVPRYGLDGH
                     PKGQVSESEAQSAGRRLVTLEQKLLRMQAQQENISGQ"
                     /name="ProQ/FINO family protein"
     gene            3622..4968
                     /gene="yagA"
                     /locus_tag="RRL15_RS24615"
                     /old_locus_tag="RRL15_24615"
     CDS             3622..4968
                     /gene="yagA"
                     /locus_tag="RRL15_RS24615"
                     /old_locus_tag="RRL15_24615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001132032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YagA"
                     /protein_id="WP_039052405.1"
                     /translation="MPMILVKKNPRGKVIRELSSEEETAVKTVCGLKKPATMAQHNLAN
                     DLLREMREYDAWLQCDCIPGDSPAMNFAALKNNTGTLYLSSFNHEHAPECPMYRQLSGN
                     EEETSFGASRHPVSTRINYRNFLPPDDSNSVIRLQTRSAYHNGERSSVRKKRPRLGRLL
                     LSLIEDAGLNKLDSLANPRIRTNYRECLDAIRQVTLQQEYIRGRSLSEIIHFRPGMSER
                     SQERLMETLENSERHWPARRKHMFFQIFMAQHICRDAVEIHWANGNIQVFRPVRGISIN
                     GEAQGGIRPPYWVILAFCRSADGRIICSEGYAHALYQLTCPVPVDSKLERNTLTALLNV
                     ASWLKRKPGTPELSLERPLFDTEVYVNGEKKYVLPDFIVTAMAPDGKTARVVIETMGYE
                     DSDYCARKSRQHTGMKQIGVLHTDPPKWLDNDHPPFEKHMYGVFMHLRY"
                     /name="yagA"
     gene            complement(5483..5785)
                     /locus_tag="RRL15_RS24620"
                     /old_locus_tag="RRL15_24620"
     CDS             complement(5483..5785)
                     /locus_tag="RRL15_RS24620"
                     /old_locus_tag="RRL15_24620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001245884.1"
                     /translation="MRNRANTSPRDEAAFINGGTAGLDNTKKSSDKSSVVSKAKPVSIS
                     FFEENLKDIDALITAEMVSGKGRVNRSDVVRAAVEALKTLSKTEVSRLIAEVKQR"
                     /name="hypothetical protein"
     gene            complement(5782..6408)
                     /locus_tag="RRL15_RS24625"
                     /old_locus_tag="RRL15_24625"
     CDS             complement(5782..6408)
                     /locus_tag="RRL15_RS24625"
                     /old_locus_tag="RRL15_24625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010260829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family plasmid-partitioning AAA ATPase"
                     /protein_id="WP_000587689.1"
                     /translation="MIIVLGSQKGGVGKSTLAVSIAAYLMSLGNRVIIVDADDQKSVLT
                     WYNNRPEDLPHIPVTGATGNIKAMLKEHEKSYDFVIADCAGRDSAEMRSGLMAADVFIS
                     PLRPSQMDLDVVPHTCSVFTAAKDFNEDVRGYLVLNMTPTNMFVNEANEAAEVLKDYPE
                     MHLANSRVCDRKAHRDAWAESMTIFETQNDKAQQEIEALVKEVIL"
                     /name="ParA family plasmid-partitioning AAA ATPase"
     gene            complement(6596..7867)
                     /locus_tag="RRL15_RS24630"
                     /old_locus_tag="RRL15_24630"
     CDS             complement(6596..7867)
                     /locus_tag="RRL15_RS24630"
                     /old_locus_tag="RRL15_24630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="WP_001703910.1"
                     /translation="MFALADINSFYASCEKVFRPDLRNEPVIVLSNNDGCVIARSPEAK
                     ALGIKMGQPWFQVRQMRLEKKIHVFSSNYALYHSMSQRVMAVLESLSPAVEPYSIDEMF
                     IDLRGINHCISPDFFGHQLREQVKSWTGLTMGVGIAPTKTLAKSAQWATKQWPQFSGVV
                     ALTAENRNRTLKLLGLQPVGEVWGVGRRLTEKLNALGINTALQLAQANTAFIRKNFSVI
                     LERTVRELNGESCISLEEAPPAKQQIVCSRSFGERITDKDAMHQAVVQYAERAAEKLRG
                     ERQYCRQVTTFVRTSPFAVKEPCYSNAAVEKLPLPTQDSRDIIAAACRALNHVWREGYR
                     YMKAGVMLADFTPSGIAQPGLFDEIQPRKNSEKLMKTLDELNQSGKGTVWFAGRGTAPE
                     WQMKREMLSPAYTTRWTDLPVAQL"
                     /name="Y-family DNA polymerase"
     gene            complement(7867..8286)
                     /gene="umuD"
                     /locus_tag="RRL15_RS24635"
                     /old_locus_tag="RRL15_24635"
     CDS             complement(7867..8286)
                     /gene="umuD"
                     /locus_tag="RRL15_RS24635"
                     /old_locus_tag="RRL15_24635"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457137.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="WP_057109540.1"
                     /translation="MVSCFPHRSRLQQTQFADRCQAGFPSPATDYAEQELDLNSYCISR
                     PAATFFLRASGESMNQAGVQNGDLLVVDRAEKPQHGDIVIAEIDGEFTVKRLLLRPRPA
                     LEPVSDSPEFRTLYPENICIFGVVTHVIHRTRELR"
                     /name="umuD"
     gene            8644..10545
                     /gene="ltrA"
                     /locus_tag="RRL15_RS24640"
                     /old_locus_tag="RRL15_24640"
     CDS             8644..10545
                     /gene="ltrA"
                     /locus_tag="RRL15_RS24640"
                     /old_locus_tag="RRL15_24640"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936286.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_000936285.1"
                     /translation="MLPDELEKRLDGIEDASKKGYPVRNLYKIACEPGLWLQAYVNIRA
                     NAGALTQGVSTDTIDGFSIERAEKLAKTLRSREYVAKPVRRVQIPKKDGKTRPLGIPGG
                     DDKLAQEVARIILERVYEPVFSEHSHGFRPKRSCETALRSIRPVWNGVKWIVDVDIKGF
                     FDNIPHKLLLNTLAEKIKDKNFLKLIEQWLKAGYVDNWKYHRSYSGTPQGGIISPLLAN
                     IYLDKLDRFVEQNLIPAYTSGTKRRANPDMNRLAHKIHKLRKKVDGMATDSESEKEAIK
                     KEIERLLEEKRSIPSQVMNDPNFRRMYYVRYADDFVIGVIGSKKDAEHISRQVRNFITT
                     SLGLEVNEAKTRIRHISEGVNFLGYEIRQADAKKLLKQKMQGRHALRRSTTGIVQLFVP
                     DNIAAKFCHQKKYGCYENVKAVHRSSLQNLSEAEIVLTFNAEMRGLANYYSLALDMKYK
                     LSKLYFIWQISLFKTLANKRRSSVNKVAKSLRQNNGDLAITVQAKNGSRKIEVFKLKHV
                     NRNRTTIVDTEPRTAHITTRTTEIMRRLGARICEYCAKTGRCEVHHVRKLKDLKKGRKA
                     GYKPSLWQLMMIARRRKTMILCASCHDKLHSGKLPDLRQEKGRILLQPPVSSGDSAK"
                     /name="ltrA"
     gene            <10728..>11621
                     /locus_tag="RRL15_RS24645"
                     /old_locus_tag="RRL15_24645"
                     /pseudo=""
     CDS             <10728..>11621
                     /locus_tag="RRL15_RS24645"
                     /old_locus_tag="RRL15_24645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            <11616..11969
                     /locus_tag="RRL15_RS24650"
                     /old_locus_tag="RRL15_24650"
                     /pseudo=""
     CDS             <11616..11969
                     /locus_tag="RRL15_RS24650"
                     /old_locus_tag="RRL15_24650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /name="plasmid SOS inhibition protein A"
     gene            11966..12562
                     /locus_tag="RRL15_RS24655"
                     /old_locus_tag="RRL15_24655"
     CDS             11966..12562
                     /locus_tag="RRL15_RS24655"
                     /old_locus_tag="RRL15_24655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000977995.1"
                     /translation="MMKSDEKYQVPAWMRPLLPLLCNTGGNDPEELLNDTETTASANVV
                     RYVLIVAVRSQVDLLQLLYRKGLLRTEIPGGFSPEEAQALLDNLVRSHISKALSGERMA
                     ARDRNADLAWIGQQLVDAAWFVRATLEAHGMSVGNESPPAPPETMPDIQTRELVMLIKR
                     LASSLKAVKPDSCVVREAQDWLRDRKLVDITDILR"
                     /name="hypothetical protein"
     gene            complement(<13045..13404)
                     /locus_tag="RRL15_RS24660"
                     /old_locus_tag="RRL15_24660"
                     /pseudo=""
     CDS             complement(<13045..13404)
                     /locus_tag="RRL15_RS24660"
                     /old_locus_tag="RRL15_24660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151583.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /name="DsbC family protein"
     gene            complement(13423..14631)
                     /gene="trbA"
                     /locus_tag="RRL15_RS24665"
                     /old_locus_tag="RRL15_24665"
     CDS             complement(13423..14631)
                     /gene="trbA"
                     /locus_tag="RRL15_RS24665"
                     /old_locus_tag="RRL15_24665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbA"
                     /protein_id="WP_011264049.1"
                     /translation="MSYNRQPVAEDPMQIWGAVGVLLILLLFVIWLFLPEVVYASCLIL
                     HTLWGLVDWGPFHNYAAPRYNLLAMTGNNAANISYSQWVNVMEQTIGILWMYLLPVTLW
                     CLWEWYQHPGQSRFTRRPVDITRLPHIFASLSPAIAPVLEDGDPEKLFHGGKRPERRVA
                     LTPEAFVEQHTLITNMQLDVAAARRCFMAQLGKPLTSWKDMAPHEKALFAIFGLQYFLD
                     DRKAALKLMDTLNLSCRIKSKRDSGKFCTPVYSLAKSAFKRVIKSDGAQQWLKQHRYVR
                     SGLVWLYAHDLRLTPPNWIWLKGVDRTLFYALHRANTTKGFIEGAGVVAVARAEAEAMR
                     FGLPCPEPCVDEAVEGLRRDMLSLGLIWDEPQPDRDRKRRILTNWSLTDDILPRTPATD
                     NEF"
                     /name="trbA"
     gene            complement(14938..15720)
                     /gene="finQ"
                     /locus_tag="RRL15_RS24670"
                     /old_locus_tag="RRL15_24670"
     CDS             complement(14938..15720)
                     /gene="finQ"
                     /locus_tag="RRL15_RS24670"
                     /old_locus_tag="RRL15_24670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023063753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein FinQ"
                     /protein_id="WP_023063753.1"
                     /translation="MMKGFLKPESRDNIPAQILKELPSWSIYVSAENLHTILPTSYPIH
                     GFFFYPFLDNNGNIIRLFIIDDLKQSQGTTGLKEKNVDVVNNIIRIKDSREGLLDSRKM
                     ECIDGEVVVTVNEKLKDFRDREFNLLNAQISMVLYICSQINDIKEKNQFKRSEKHKKHV
                     HTHHELPAQNIREWDVGIRMGQAIRQYRQAEPTGKERTTIGSKRPHIRRGHWHTYWTGS
                     KKPELAHERKPRLIWLPPVPVNLEDVNKLPVVITPIDK"
                     /name="finQ"
     gene            15916..16233
                     /gene="sugE"
                     /locus_tag="RRL15_RS24675"
                     /old_locus_tag="RRL15_24675"
     CDS             15916..16233
                     /gene="sugE"
                     /locus_tag="RRL15_RS24675"
                     /old_locus_tag="RRL15_24675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463202.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="WP_000118520.1"
                     /translation="MSWIVLLIAGLLEVVWAIGLKYTHGFTRLTPSIITIAAMIVSIAM
                     LSWAMRTLPVGTAYAVWTGIGAVGAAITGILLLGESASPARLLSLGLIVAGIIGLKLST
                     H"
                     /name="sugE"
     gene            complement(16230..16763)
                     /locus_tag="RRL15_RS24680"
                     /old_locus_tag="RRL15_24680"
     CDS             complement(16230..16763)
                     /locus_tag="RRL15_RS24680"
                     /old_locus_tag="RRL15_24680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="WP_001221666.1"
                     /translation="MRILPVVAAVTAAFLVVACSSPTPPKGVTVVNNFDAKRYLGTWYE
                     IARFDHRFERGLDKVTATYSLRDDGGINVINKGYNPDREMWQKTEGKAYFTGDPSRAAL
                     KVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEMKQQMLAIATREG
                     FEVNKLIWVKQPGA"
                     /name="lipocalin family protein"
     gene            complement(16857..18002)
                     /locus_tag="RRL15_RS24685"
                     /old_locus_tag="RRL15_24685"
     CDS             complement(16857..18002)
                     /locus_tag="RRL15_RS24685"
                     /old_locus_tag="RRL15_24685"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extended-spectrum class C beta-lactamase CMY-42"
                     /protein_id="WP_039023136.1"
                     /translation="MMKKSLCCALLLTASFSTFAAAKTEQQIADIVNRTITPLMQEQAI
                     PGMAVAVIYQGKPYYFTWGKADIANNHPVTQQTLFELGSVSKTFNGVLGGDAIARGEIK
                     LSDPVTKYWPELTGKQWQGIRLLHLATYTAGGLPLQIPDDVRDKAALLHFYQNWQPQWT
                     PGAKRLYANSSIGLFGALAVKPSGMSYEEAMTRRVLQPLKLAHTWITVPQNEQKDYAWG
                     YREGKPVHSSPGQLDAEAYGVKSSVIDMARWVQANMDASHVQEKTLQQGIALAQSRYWR
                     IGDMYQGLGWEMLNWPLKADSIINGSDSKVALAALPAVEVNPPAPAVKASWVHKTGSTG
                     GFGSYVAFVPEKNLGIVMLANKSYPNPVRVEAAWRILEKLQ"
                     /name="extended-spectrum class C beta-lactamase CMY-42"
     gene            complement(18455..19152)
                     /locus_tag="RRL15_RS24690"
                     /old_locus_tag="RRL15_24690"
     CDS             complement(join(18455..18903,18903..19152))
                     /locus_tag="RRL15_RS24690"
                     /old_locus_tag="RRL15_24690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558260.1"
                     /ribosomal_slippage=""
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1B family transposase"
                     /protein_id="WP_095033700.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTIFRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEQWGYVGAKSRQRWLFYAYDRLRKTVVAHVFGERTMATLGRLMSLLSPFDVVIW
                     MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1-like element IS1B family transposase"
     gene            <19207..19305
                     /locus_tag="RRL15_RS24695"
                     /old_locus_tag="RRL15_24695"
                     /pseudo=""
     CDS             <19207..19305
                     /locus_tag="RRL15_RS24695"
                     /old_locus_tag="RRL15_24695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480010.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutE"
                     /name="ethanolamine utilization protein EutE"
     gene            complement(19377..19586)
                     /locus_tag="RRL15_RS24700"
                     /old_locus_tag="RRL15_24700"
     CDS             complement(19377..19586)
                     /locus_tag="RRL15_RS24700"
                     /old_locus_tag="RRL15_24700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062602.1"
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="WP_000062603.1"
                     /translation="MSMPPAIANTFLFEMMKSKSKDVTLAAIYALGEGRCQAENITREL
                     HRLSQSDDMEIKIAAIKALGRIYR"
                     /name="HEAT repeat domain-containing protein"
     gene            19978..20154
                     /locus_tag="RRL15_RS24705"
                     /old_locus_tag="RRL15_24705"
     CDS             19978..20154
                     /locus_tag="RRL15_RS24705"
                     /old_locus_tag="RRL15_24705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001054897.1"
                     /translation="MNLVDAFVKKVISEPYEEYGKWWIDVEYISWGVPGKTRLMFESKE
                     QALEVKEGYKFLT"
                     /name="hypothetical protein"
     gene            complement(20219..20314)
                     /gene="dqlB"
                     /locus_tag="RRL15_RS24710"
                     /old_locus_tag="RRL15_24710"
     CDS             complement(20219..20314)
                     /gene="dqlB"
                     /locus_tag="RRL15_RS24710"
                     /old_locus_tag="RRL15_24710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="WP_000609148.1"
                     /translation="MINLLIIVLRAVVAVANALIAVLELIRELID"
                     /name="dqlB"
     gene            20815..21066
                     /locus_tag="RRL15_RS24715"
                     /old_locus_tag="RRL15_24715"
     CDS             20815..21066
                     /locus_tag="RRL15_RS24715"
                     /old_locus_tag="RRL15_24715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001291964.1"
                     /translation="MSEKWSKEFDSCFQVGSIEEIADALEKRSGSEENALAMLAMTAFT
                     LMTRRGICEMQNVAPDGKGIRLELIGFRENETIPDTLH"
                     /name="hypothetical protein"
     gene            complement(21138..21290)
                     /locus_tag="RRL15_RS24720"
                     /old_locus_tag="RRL15_24720"
     CDS             complement(21138..21290)
                     /locus_tag="RRL15_RS24720"
                     /old_locus_tag="RRL15_24720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618166.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="WP_001387489.1"
                     /translation="MPQRTFLMMLIVVCVTILCFVWMVRDSLCGLRLQQGNTVLVATLA
                     YEVKR"
                     /name="Hok/Gef family protein"
     gene            21611..21985
                     /locus_tag="RRL15_RS24725"
                     /old_locus_tag="RRL15_24725"
     CDS             21611..21985
                     /locus_tag="RRL15_RS24725"
                     /old_locus_tag="RRL15_24725"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_223349261.1"
                     /translation="MSQLKQPIFLKKIKKVINTIPGLEEQIFACRNKKRSDNPLLFIDR
                     KDEERILMSRLQSQQKNEELASKLESLFHGNELSSPHSILCFIYWRYTKKIYRLSEDII
                     SDVANTYVDNLDSTSVLQKA"
                     /name="hypothetical protein"
     gene            22138..>23295
                     /locus_tag="RRL15_RS24730"
                     /old_locus_tag="RRL15_24730"
                     /pseudo=""
     CDS             22138..>23295
                     /locus_tag="RRL15_RS24730"
                     /old_locus_tag="RRL15_24730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_031587700.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element ISEcp1 family transposase"
                     /name="IS1380-like element ISEcp1 family transposase"
     gene            23351..24048
                     /locus_tag="RRL15_RS24735"
                     /old_locus_tag="RRL15_24735"
     CDS             join(23351..23600,23600..24048)
                     /locus_tag="RRL15_RS24735"
                     /old_locus_tag="RRL15_24735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558260.1"
                     /ribosomal_slippage=""
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1B family transposase"
                     /protein_id="WP_095033700.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTIFRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEQWGYVGAKSRQRWLFYAYDRLRKTVVAHVFGERTMATLGRLMSLLSPFDVVIW
                     MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1-like element IS1B family transposase"
     gene            complement(<24062..24367)
                     /locus_tag="RRL15_RS24740"
                     /old_locus_tag="RRL15_24740"
                     /pseudo=""
     CDS             complement(<24062..24367)
                     /locus_tag="RRL15_RS24740"
                     /old_locus_tag="RRL15_24740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303319.1"
                     /GO_process="GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]; GO:0006355 - regulation of
                     DNA-templated transcription [Evidence IEA]; GO:0140673 -
                     transcription elongation-coupled chromatin remodeling
                     [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /name="transcription termination/antitermination NusG
                     family protein"
     gene            complement(24809..25096)
                     /locus_tag="RRL15_RS24745"
                     /old_locus_tag="RRL15_24745"
     CDS             complement(24809..25096)
                     /locus_tag="RRL15_RS24745"
                     /old_locus_tag="RRL15_24745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraA"
                     /protein_id="WP_000074855.1"
                     /translation="MSPQMIQKLPAITLLEGMFPELSTNQLKVCVFYAMGVPYDAIAQN
                     CRLSPETVRTYLKRSLKNLNLEGYDALRSAVLMRTFVFMISNTAKENEKM"
                     /name="conjugal transfer protein TraA"
     gene            complement(<25134..25292)
                     /locus_tag="RRL15_RS24750"
                     /old_locus_tag="RRL15_24750"
                     /pseudo=""
     CDS             complement(<25134..25292)
                     /locus_tag="RRL15_RS24750"
                     /old_locus_tag="RRL15_24750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000980235.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /name="ash family protein"
     ARG             complement(16861..18002)
                     /name="blaCMY-42"
                     /gene="blaCMY-42"
                     /gene_name="extended-spectrum class C beta-lactamase
                     CMY-42"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          2..1032
                     /name="IncI-gamma/K1"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gtggcaggtc tgaagaatac tccatataac gcagtacact ggagtcagtt agcacccgaa
       61 gagcagatcc gtttctggga agactatgaa gcgggaaggg cgaccacttt cctggttgaa
      121 ccggaaagga agcgcacgaa gcgccgtcgc ggtgagcact ccaccaaacc caaatgcgaa
      181 aatccgtcct ggtatcgtcc tgagctctat aaggcgctga gcgggcagct cgggcacgcc
      241 tacaaccgtc tggtgaaaaa ggacccagtg accggcgagc agagcctgcg catgcacatg
      301 tctctgcatc ctttttacgt gcagaaacga acgtatgccg gtcgcaaata tgctttccgt
      361 ccggaaaaac aacgcctcct cgatgccatc tggccggttc tggtcagctt cagtgatgcg
      421 ggcacacata ccgtaggcat gagtgtttcc cgtctggcca gagaaatcag cccgaaagac
      481 agcaagggga aggttattcc ggaactggaa gtgacggtct cccgcctttc ccgtttgctg
      541 gccgaacagg tacgttttgg tgtgctgggt gtttcagagg aaaccctgtg ggaccgtgaa
      601 acccgccagc gtctgccacg ttacgtctgg ataacaccgg caggctggca gatgctgggc
      661 gtcgacatgg tgaaacttca cgaacagcag cagaagcggt tgcgtgaaag tgaaatccgc
      721 cagcaactca tccgggaagg cgtactgcgt gaggatgaag atatctccgt acatgcggcc
      781 agaaaacgct ggtatctgca gcgcagccag gatgcactga aacaccgtcg tgcaaaagcg
      841 gcagccagta agcgcgccag acgtctgaag aaactgcctg ccgaccagca gattcatgag
      901 atggcagagt atctcaggaa gcgtctgccg ccggatgaag cctatttttg ttccgatgac
      961 catctgaagc gactggccat cagggagttg cgtcagcttg aactgacgct ggctgcgcca
     1021 ccgccgcact agacagcacc attccttcag cactgaatca tcgccagccc ctccggggct
     1081 ttcggcgctg gttccgctca gcccaaaatc tgcagtaatc accttaaatc ccctcagagg
     1141 ggcatatctg tccataaaac cacgtatcag tcatcagaac atggccacgt tgtttcagtt
     1201 atccacataa atccgcaaac aaagaacttt aaaaagctgc aaacctgaaa cagcaaacct
     1261 gcaatatagt cctaccccca ttatttaatc ccctgcgttg cttcgccgca gggaaagtct
     1321 ttatctctga aaccactgtg aacaaataca gagaggcctt cgcttgcagc ggccaaggcc
     1381 gcgccgctca gaatctaaaa gcacctccca cgctgacgcg cgggccccga acctcaccgt
     1441 tctgaaacca caacaaaaag acatcaggaa taaaaacacc gcacaaacgc agcaccgtgc
     1501 ctacccctca taactgaaaa gcgaggccgc ccccgcccaa agggcgggaa caacatcgct
     1561 tttaattatg aatgttgtaa ctacattatc atcgctgcca gtcttctggc tggaagtcct
     1621 ccgtacacgc tcgtaagcgg ccctgacggc ccgctaacgc ggagatacgc cccgacttcg
     1681 ggtaaaccct cgtcgggacc actccgaccg cgcacagaag ctctctcatg gctgaaaacg
     1741 ggtatggtct ggcagggctg gggatgggta aggtgaaatc tatcagtccg taccggctta
     1801 cgccgggctt cggcggtttt actcctgtgt catatgcaac aacagagtgc cgccttccat
     1861 gccgctgacg cggcatatcc tggtgacgat atctgaatcg ttatatactg tgtataatat
     1921 atggtgcgtt aatttttaaa cccttattta atttcctcgt cagttagata ttgtccgttt
     1981 cccaaacgaa gcccttcaga gacgtcgtgt aaacacggtc cttttatctt acaggttaac
     2041 ccctatcaat cttaggatga aaccacgcca tcctctgctt gcctggttat taatttcata
     2101 tcatcttatg ccttttttat cttcatttgg gaccgtactt ttcttcggaa tatcactatt
     2161 cgttaactgc tgttcatcaa taaaattcct gatgatattc agttcactct gacggatttt
     2221 ttcttcttca ttttttctca gaagacggca gatatccatt tcatcccgcc cggtcattct
     2281 ttttccggaa aaaaggcggt taagcagctc ctgctttaca tctggtgagt ctttggtggc
     2341 ctccatgagg ttgagcagac tctgacgatc gagaatagag aaaacttttg ggaaataatg
     2401 taaaaggaaa agaacccaga atgctgtcca tgcaaagcag agaaaagctg ctgtaagcac
     2461 gtgattcttg cctgataggt atataatcag cagaacaacc gaaatccaag atgccagttc
     2521 taaaaaagta tatacaggct tcaggcgtct gtatgcctgt tcatgattat tcaggatgaa
     2581 ttgaattttt tctgcacctg atttactcat aataattctc ttcccatttt tctgatacgt
     2641 aatgtccagc tgttatcagg agtgtttatt gcccagaaat attctcctgc tgtgcctgca
     2701 tcctgagcaa cttttgttct aatgtcacca gtctgcgacc ggcactctgt gcttcagatt
     2761 ctgagacctg ccctttgggg tgaccatcca gaccataacg tgggacgccg gcgacagctg
     2821 tccgctgcag atatacacgg cgtgaggtga tgcaggacac tgcacagagc cattcgtcca
     2881 tggtcagttc tgtctccggg tgttctgcca gaaatgctga aatatcattg tttatgccaa
     2941 ttttcagggg aataaatcca ccaccttcag cacgaagatg aggaaacagg atctttattt
     3001 tttccacgca ctcgtgagta tactctctgg tccagtggga gggacgtggg aaacggcgtt
     3061 ttttcttttt cttcttcctg gctggtgctt cggggatatg tgtttcgacg gcagatgaat
     3121 ttattccgac gggggctgat tctgctgcct gttcagtaaa tgcagattcg ataatatctg
     3181 ttttttcgga taaggaaggt aacgaaaagg tacgacgttt tttcactaca atgacgggga
     3241 ttttcctttc ttcctgattt ttttccataa atacctcggc tggctggttt tactctttaa
     3301 taatatcaca atccataaat tagtaaattt tccgtcaggt gatttttcat ggtttataag
     3361 tccgttctct gaatatttat actcgaggcg gtaacagtta tggcgtgaca gtcgaattga
     3421 tattcgactg aaccctgacc gaaacatggt tgatatattc tctaacgtta atgttgtcat
     3481 gatataaata aaaaaaattc atttgaaagg atgtcagaag aagatatcgg ggcgcacagt
     3541 tcatctctgc ctttgtttca tggtgtgttc tttgtatatt ttacttactg tttaagcatg
     3601 tacataagga gtgctttcat tatgccaatg atacttgtaa agaagaatcc ccggggcaaa
     3661 gtgattcggg agcttagcag tgaggaagag actgcagtaa agacggtctg tggactgaaa
     3721 aagccagcaa ccatggcaca acataacctg gccaatgatc ttctccggga aatgcgtgag
     3781 tatgatgcat ggttacagtg tgactgtatt ccgggagatt cgccggccat gaattttgct
     3841 gctcttaaaa acaatacggg tactctttat ctgtccagtt ttaatcatga gcatgcacct
     3901 gaatgcccca tgtatcgtca gttatcagga aatgaagagg aaacatcctt cggagcaagc
     3961 cggcatcccg ttagcacccg tataaattac agaaactttt taccacctga tgacagtaat
     4021 tctgttatca gactgcagac ccgctctgct taccataatg gtgagcgctc ctccgttcgc
     4081 aagaaacgtc cgcgccttgg ccgtttactc ctgtcgctta ttgaagatgc agggctgaat
     4141 aaactggatt ccctggctaa tccccgtatc cggactaatt atcgtgagtg tcttgatgcc
     4201 atacggcagg ttacactaca gcaggagtat attcggggaa gatccttatc tgaaattatc
     4261 catttccggc caggaatgtc tgaaaggtct caggagcggt taatggaaac gctggagaat
     4321 tcagaacgac actggcctgc cagaagaaaa catatgttct ttcagatttt tatggcgcag
     4381 cacatctgtc gtgatgctgt ggaaattcac tgggcgaacg gtaatattca ggtcttcaga
     4441 cctgtgcggg ggatcagtat taatggtgaa gcgcagggcg gaatacgtcc cccttactgg
     4501 gttattcttg ctttttgccg gagtgccgat ggcagaatca tctgcagtga agggtatgct
     4561 catgctcttt atcaactgac atgtccggtt cctgtggaca gcaaactgga acgaaacacg
     4621 ctcactgctc ttctgaatgt ggccagctgg cttaaaagaa agccaggtac gccggaatta
     4681 agtctggaaa ggcccctgtt tgatacagaa gtttatgtta atggtgaaaa gaaatatgta
     4741 ctgccggatt tcattgtcac agcaatggct cctgacggaa agacggccag agtggtcatc
     4801 gaaacgatgg gatatgaaga cagtgattac tgcgcgagaa aatccaggca gcataccggc
     4861 atgaagcaga ttggtgttct gcataccgat ccaccgaaat ggctggataa cgatcatccc
     4921 ccttttgaga aacatatgta cggtgttttt atgcatctca ggtactgaga tattttgtgg
     4981 ctcagttctg taacttttcc cgtaacattg tctgttgtta cgggaaagtc cggtttttgt
     5041 attgcaccag agaatactca gactgtgatg ctgccacagc gtcagcaggc tttctgaacg
     5101 gtgtaacatc acttcatgtt aatgataatc actatcatta aatcttgaca tgccattttc
     5161 tccttaataa attaatactg tatatgtatc catatacgta agcagttaat tcatttgttt
     5221 tcctcagagg atgaaggaga taccgaatgt ctgatcctgt acggggttca gtgagttagc
     5281 tggcctggat gtaaccactc gttcaactca attctcttag cgtgggaaaa tttccgcttt
     5341 tccaagcgga cccctattaa tatttgtttt gttttaggtt ctattcaaca taggtcatcc
     5401 taaaatacat tgaagataac aagataacaa gataacaaga taacaagata acaagataac
     5461 aagataacaa gatagtaagg tgttatcttt gttttacttc tgctatcaat cgtgatacct
     5521 ctgtttttga tagtgtttta agtgcttcta ccgccgccct gacaacatca gaacgattca
     5581 ctctgccttt acctgaaacc atctcagccg ttataagggc atcaatgtct tttaggtttt
     5641 cttcaaaaaa agaaatactc actggttttg ctttgcttac aaccgatgat ttatctgatg
     5701 actttttggt attatcaagg ccagccgtgc caccgttaat aaatgctgcc tcgtcacgag
     5761 gtgaagtgtt tgctctattt ctcacagaat aacctctttc actaacgctt cgatttcttg
     5821 ctgggcttta tcattttgcg tttcaaaaat tgtcattgat tcggcccaag catctcgatg
     5881 tgctttacga tcacataccc gggagtttgc cagatgcatt tcagggtaat ccttaagtac
     5941 ttcagctgcc tcattggctt cattaacaaa catatttgtg ggcgtcatgt ttaaaacgag
     6001 atatccccga acatcctcat taaaatcttt tgctgctgta aaaacggagc atgtatgagg
     6061 cacaacatcc aggtccatct gagatgggcg caaaggagaa atgaatacat ctgcggccat
     6121 caatccacta cgcatttctg cgctatcacg gccagcacag tctgcaataa caaaatcata
     6181 ggatttttcg tgttccttta acatggcttt gatgttacct gtcgcgcctg taaccggaat
     6241 atgagggaga tcttcagggc gattgttgta ccaggttaat actgatttct ggtcatctgc
     6301 gtcgacaata atcactctat tgcctagtga catcagatac gcagctatag ataccgccag
     6361 tgtactttta cctaccccac ccttttgact acctaatact attatcatta tagcctcata
     6421 ttaagctgtt aagttaacaa cttaatattg ccatctgaac gcgaaccatg caattatttt
     6481 tgttatgtta acaacttaac aagctgttaa gtatttgatt tatagattaa ttaagataac
     6541 aagataacaa gataacaaga taacaagata acaatattat gggtggatta catattcata
     6601 actgggctac tggcaaatct gtccatctgg tggtatacgc aggagaaagc atttcccgtt
     6661 tcatttgcca ctcaggggcg gttcctcgcc ccgcaaacca cactgtccct tttcccgact
     6721 ggttcagttc atcgagtgtt ttcattaact tttcactgtt tttacggggc tggatttcat
     6781 caaataatcc cggctgtgct atacccgatg gtgtgaaatc agccagcatg actcctgcct
     6841 tcatatagcg gtacccttca cgccagacat ggtttaaggc tctgcatgcg gcggcaataa
     6901 tgtcccggct gtcctgtgtg ggcaatggaa gcttttccac agcggcattg ctgtaacagg
     6961 gttcttttac tgcaaagggg gatgtccgta caaatgtcgt cacctgccgg caatactgac
     7021 gctccccacg tagtttctct gcggcccgct ctgcatactg aacaacagcc tggtgcatgg
     7081 catctttgtc tgtgattcgt tcaccaaaac tgcgactaca gacaatctgc tgttttgccg
     7141 gtggtgcttc ttccagggat atgcaggact cgccgttgag ttcgcgtacc gtacgctcaa
     7201 gaatgacgct gaagtttttc cggatgaatg ccgtgttagc ctgcgccagc tgcagtgctg
     7261 tgttaatacc cagcgcattc agcttttccg tcagtctgcg tcctactccc cagacctcac
     7321 caactggctg cagccccagt agcttcaagg tccgattacg gttttctgcc gtcagcgcga
     7381 ccactccgga aaactgtggc cattgctttg ttgcccactg tgcactttta gccagcgttt
     7441 ttgtaggcgc aatgcccacc cccatggtga gtcctgtcca gctctttacc tgttccctga
     7501 gctgatgacc aaaaaaatcc ggagagatgc aatgatttat cccccgcaaa tcaataaaca
     7561 tttcatcaat tgagtagggc tcaactgcgg gagaaagcga ctccagaaca gccatgaccc
     7621 gttggctcat gctgtggtac agcgcataat tgctggaaaa tacatgtatt ttcttctcca
     7681 ggcgcatctg tctcacctga aaccagggct gccccatttt gatgccaagg gcttttgcct
     7741 ccgggctgcg cgcgatcaca cagccatcgt tattgctgag tacgatgacc ggttcgttgc
     7801 gaaggtccgg gcggaaaact ttttcacatg aggcgtagaa actgttgata tcagccagtg
     7861 caaacatcag cgtaactccc gcgtcctgtg tatcacgtga gtgacaacac caaaaataca
     7921 gatgttttcc ggatacagtg tgcggaactc cgggctgtct gaaaccggct ccagtgccgg
     7981 gcgtgggcgc aacagcagtc gtttgacggt gaactcaccg tcgatctcag cgataacgat
     8041 gtccccgtgt tgtggttttt cggccctgtc cactaccagc agatcaccat tctgcacgcc
     8101 agcctggttc atcgattcac cgctggcgcg cagaaagaag gtggctgcag gtctgctgat
     8161 gcaatagctg ttcagatcca gttcctgctc agcataatca gtggcaggcg aaggaaaacc
     8221 ggcctggcaa cgatcggcaa actgggtttg ttgaagtcga gatctatgtg ggaaacagga
     8281 aaccaataac gcagaacgat tgaagcgtta ttcccgtaat agcacaagaa atccgcccta
     8341 cgttgattgt gaccactcta acgggccagc tattgatgaa atagttaaac gatcgaaaga
     8401 catcctaagt acatcgcatc tccacaccgt gaaactacgg gactacctaa actggattta
     8461 tacccagcat ggtagcggag tctccatagt actcaaggtc ggcctgtaat gggctacggc
     8521 agtccgcatt atacggaagc agcaaaggta taaatcaacc ggaagggaga tgaaagcctc
     8581 acagtgctgc ggggaaggga gacagttgtt agatacatca atctctaaga ggagggttag
     8641 ccaatgctgc ctgatgaact tgagaaaaga ttagatggaa ttgaggatgc ttcaaaaaaa
     8701 gggtatccag tacgtaatct atacaaaatt gcgtgtgagc ccggactgtg gctgcaagcc
     8761 tacgtgaata tacgggccaa tgcaggtgca ctgactcaag gtgtatcaac agataccatt
     8821 gatggcttct ctatcgaaag agcagagaag ctggcaaaaa cattgcggag tagagaatac
     8881 gtagcgaaac ctgtacgcag agttcagatc ccaaaaaagg acggaaaaac cagaccgctg
     8941 gggattccag gtggagacga taaactggcg caagaagtag caagaattat tctggaaagg
     9001 gtttatgaac cagtattctc tgaacactct catggattca gaccaaaacg ttcgtgtgaa
     9061 actgcgctca gatctattcg tccagtctgg aacggagtta aatggatcgt ggatgttgat
     9121 atcaaaggtt tctttgacaa tatcccccac aaactattgc tgaatacact ggcagagaaa
     9181 attaaggata agaatttcct gaaattaatc gaacagtggc tgaaggcagg atatgtggac
     9241 aattggaaat accatcgctc atattctggc acgccacaag gtgggataat ttctccctta
     9301 ctggcaaata tatacctcga caaactggac cgttttgtag aacagaactt aattcctgca
     9361 tatacaagcg gtacaaaacg aagagcgaat ccagatatga acaggctggc ccataagatt
     9421 cataaactac gaaagaaagt agacggtatg gcaactgatt ctgaatcaga aaaagaagcc
     9481 ataaagaaag aaattgaacg actgcttgaa gaaaaacgtt ctataccatc tcaggtcatg
     9541 aacgatccaa acttcaggcg tatgtactat gttcgatatg ctgatgactt cgtaattggt
     9601 gtcatcggct cgaagaaaga tgctgaacat atatcaagac aggtcagaaa ctttattact
     9661 acttcactgg gattggaagt taatgaagca aagaccagga tccgccatat ctcagaagga
     9721 gttaattttt taggctatga aatcagacag gcagatgcaa agaaattgct gaagcagaaa
     9781 atgcaagggc ggcacgctct ccggagatcc accacaggaa ttgtgcagct cttcgttcct
     9841 gacaatatag ccgctaaatt ttgtcaccaa aagaaatatg gctgttatga gaatgtgaag
     9901 gctgtacatc gttcaagtct acaaaatctc agtgaagctg aaattgttct gactttcaac
     9961 gcggaaatgc gcggattggc gaactactac agtctggcgc tggacatgaa atataaatta
    10021 tcaaaattat atttcatatg gcaaataagc ctttttaaga ccctagcgaa taaacgacgt
    10081 agttcagtga ataaagtcgc caaatcgcta cggcaaaata atggagatct ggctattacc
    10141 gttcaggcca aaaatggatc ccgaaagatt gaggttttca aactcaaaca tgtcaatcgc
    10201 aaccgaacaa cgattgtcga tacggaaccg agaaccgcac atatcacaac ccgcacaaca
    10261 gagatcatgc gtcgattagg ggcaaggatc tgcgagtatt gtgcgaagac tgggcgctgc
    10321 gaagtacatc atgtcagaaa gctaaaagac ctgaagaaag gtcgtaaggc cggatataaa
    10381 cccagcctgt ggcaactaat gatgatcgca cgccgccgga aaacgatgat tttgtgcgca
    10441 agttgccacg acaagttaca tagcggaaaa ctacccgatc tgagacagga aaagggaaga
    10501 atactcctgc aaccacctgt ctcaagtggg gattctgcta aatgagttta tccccaataa
    10561 gctagcaatg gagagccgta tgcattgaaa ggtgcacgta cggttcggag ggggggtaac
    10621 ggtgatctaa ctcaagataa gcaggacata ttcgccattt atcttgaaag accacacgta
    10681 ccctacccta ccgtctgggt aatgatccgg aagtccgtta catccccaaa ccggatgtgc
    10741 gggttatccg caatctgatt acggaaagtg aagtggcggt ggcggggaac agtaaattcc
    10801 gcttcgtggg ggctgatgcc ttctcgccag acgaactgcg caccgatttg ttcagcgacg
    10861 acgagggtgg ctatgtggac tgcgtggcgc tcgatgccgc cctgctggaa aaactccagg
    10921 ctgtcgcaga gttccttcgg gaagccgaag gctgggaatg gtgcgccgga cgcatggagc
    10981 ctgtcggtga gtgccgtgag gatgccggaa cataccgctg tctgccggag ccggaagcgg
    11041 tgctgaccga ggcggaagaa gaacgtctga acgaactgat ggcacgttac gacgcgctgg
    11101 aaaaccagtg tgaggaatct gacctgctgg aagcagaaat gaagctgatg cgctgcatgg
    11161 cgaaggtcag agcgtggacg ccggagatgc gtgccggagg tggagtggtg gtgtcctggc
    11221 gttatggcaa cgtgtgtgtc cagcgtggtg tgcagttgcg cagtgaagat gacgcgactg
    11281 atgacgctga ccgcacggaa caggtgcagg agaaagcgtc agtggaggca atcagtctgc
    11341 cgttgctgac gaaaatgtct tccgaacgca cgctggcagt ccaggcagca ctgatgcagc
    11401 aaccggacaa atctcttgca ctgttggcat ggacgctctg cctgaatgtg tttgacagcg
    11461 gagcgtacag taaaccagca caaatcagcc tggaatgtaa acattattcg ctgaccagcg
    11521 atgcgccgtc agggaaggaa ggtgccgcat tcctggcgct gatggcagaa aaagcccgtc
    11581 ttgcggccct gttaccggag ggatggtcac gggacatgac agagcagaaa tacagccgcc
    11641 agcgggaacg tgaggcagaa cgacgggaac tggaatacca gacatgtttt gctcaggcgc
    11701 agattgacct tgcgtttcat actcccgcca cggtcggaag ctggttgtcc cgctggtctg
    11761 gtgttgttga ggagcatgat ctggaaacga ttttctgggg gtggtgcggg cgttttccat
    11821 cactgtcatc atttgaccgg tttttctggc aggaggaacc actctggcgg ctgatttttg
    11881 aagccggtga ggccggtcgt ggtgcaccgg tacaggtacg tgcacttgag cagtggatga
    11941 tcccgaacaa gctggagaac gtaatatgat gaaatcagac gaaaaatacc aggttcccgc
    12001 gtggatgcga cctctgttgc cgttgctctg caacaccggg gggaacgatc cggaagaact
    12061 gctgaatgat acagaaacca ctgccagtgc gaatgttgtc cgttatgtac tgatagttgc
    12121 tgtgcggtcg caggttgatc tgctgcagct tctgtacagg aaagggctgt tgcgcacaga
    12181 gataccaggt ggcttttcac cggaagaagc gcaggcactc ctggataatc tggtgcgcag
    12241 ccatatcagc aaggcgctgt cgggcgagcg aatggcagcc cgtgacagaa atgccgatct
    12301 ggcctggatt ggacagcaac tggtcgatgc cgcctggttt gtccgtgcca cactggaagc
    12361 gcatgggatg agcgtcggaa atgagagtcc ccctgctccg ccggagacaa tgccggacat
    12421 acagacacgg gaactggtga tgttgatcaa gcgactggca tcatcgctga aagcggtgaa
    12481 accggacagt tgtgtggtgc gtgaagcgca ggactggctg cgcgacagaa aacttgtgga
    12541 tatcacagat attctccggt gaaccaggca tgtatatttt acgcagggac acgccgatca
    12601 tgatcgtttc atatcagggg tggtctggag gtcatgcggc gtgtcctctg cactcgccgg
    12661 aataaggaag tcgccggcgg ctccgctttt acccggccat gcggggcatg gccttgtggg
    12721 ttttcagctc tgtggcctca gcgtcgtgtg cgggctgtgc cgtgcctcca tcttagccgg
    12781 gctggcaggg atgcaagggt acgcttcgcc gctgcggtca cccggtccct ccttcccgtc
    12841 tgccgtgatt ttccggtcgt tcgtccgctc aggtttcgcg gcctcaccct gcaactcccg
    12901 tcagccgtgt gcgtcaggct gcggcttccc ttgcgcccct gcatccccgc cttatcgccc
    12961 ggcttttatg gaggcacggc accgcccggt gtcgcagaac atgtgactat ggaggattcg
    13021 ggaatgtctg ttgttgcacc tgctgtagcg gaacagagag acggcaatca ttgccattac
    13081 gaacagtaca acgctggcga ttattgcacg gaacaaaggc gttctgctga agcggcgttc
    13141 catcaggtat tcaaggcgca gacaggaatc aatagctgta agtatctggt cagcgttatg
    13201 ctcctgcttg tcaatcagga cggtttctct gttacgagtg cccatggcgt caaaggctat
    13261 atgaatcagt tgattgtttc gtgttaccag ccctgacagg gcatcctgtg gaaggacggc
    13321 aataatttct gtcctgccac ccggccagat tgcctgcacg gaaatatgtt ttgcaatgcg
    13381 tgttgcaaag tactcgattg tcattttcag ttctccggat aatcagaatt cgttgtctgt
    13441 agcaggtgtg cggggcagga tgtcgtcggt gagggaccag ttggtcagaa tgcggcgttt
    13501 ccggtcgcga tctggttgtg gttcatccca gataagaccg agactgagca tatcccgccg
    13561 taagccttcc accgcttcat caacacaggg ttcagggcag ggaaggccaa aacgcatggc
    13621 ttcggcctct gcacgtgcaa cggccaccac gccagcgcct tcaataaatc ctttcgtggt
    13681 attggcgcgg tgtaatgcat aaaacagcgt tctgtccacg cctttcagcc aaatccagtt
    13741 gggtggcgta agccgcaagt catgtgcata cagccagacc agcccactgc ggacgtagcg
    13801 atgttgtttc agccattgct gtgctccgtc gcttttaatc actcttttga atgcagattt
    13861 ggccagtgaa taaacagggg tacagaattt cccactgtcc cgtttgcttt taattctgca
    13921 tgagaggttc agtgtatcca tgagctttaa tgctgcctta cgatcatcca ggaaatactg
    13981 aaggccaaat atggcaaaga gcgctttttc atgtggtgcc atgtctttcc aggacgttaa
    14041 aggtttaccc aactgggcca tgaaacatcg tcttgcagct gcaacatcga gctgcatatt
    14101 ggtgatgaga gtatgctgtt cgacaaatgc ttccggggtg agagcaaccc gccgttcagg
    14161 tcgtttgcca ccatgaaaga gtttttccgg atcaccatct tccagtacag gggcgatagc
    14221 aggggacaac gaagcaaaga tgtgtggcag tcgggtaata tctactggtc tgcgggtgaa
    14281 acggctttgt cccggatgtt gataccattc ccacaaacac cagagtgtca caggtaagag
    14341 gtacatccac aaaataccta tggtctgctc catgacgtta acccactggc tatagcttat
    14401 gttggcggcg ttgttaccgg tcatggcaag caggttgtat cgtggtgctg catagttatg
    14461 aaatggtccc cagtcaacca gtccccataa ggtatgaaga atcaggcagc tggcgtaaac
    14521 cacttccggc aagaataacc atatgacgaa tagcagcagg ataagaagga caccaacagc
    14581 gccccatatc tgcatcggat cttctgcgac aggctgtcga ttgtaagaca tggcaaagtc
    14641 tccatttctg gaatttaaat caaacggaaa ggttaaaaat ttattttcag gtgataagag
    14701 ctaaccagag gagttagccg tgcgataaaa ggattgttgc agatacgaaa gtcgttagaa
    14761 tagccgcgga cgcttgaggc tgtatgccga aagcgttttg tggacggtat acagcagaaa
    14821 gcccctggag atttttttat caatcaacca agggctctac tgtaatgcat gaacaacatt
    14881 acagtagcct gataatttag atatgtgtac atcctaccgg aaaagatact tctcaggtca
    14941 tttatcaatg ggcgttatta ccaccggtag tttgtttacg tcttctaaat taactggtac
    15001 cggaggtaac catattaatc taggtttacg ctcatgggct agttctggtt tcttacttcc
    15061 tgtccagtat gtatgccagt gtccccggcg tatatgagga cgtttgctac ctattgttgt
    15121 ccgctctttc cctgtgggtt ctgcttggcg atattgccgt atggcttgcc ccattcgaat
    15181 accaacgtcc cactcccgaa tgttttgtgc tggtagttca tggtgagtgt gaacatgctt
    15241 tttgtgcttt tctgaccgtt taaattgatt tttttctttt atatcattga tctgtgaaca
    15301 gatataaagc accattgata tctgtgcgtt aagaagatta aactccctgt ctctgaaatc
    15361 tttgagcttt tcgttaactg ttacgacgac ttcaccatcg atacactcca tctttctgga
    15421 gtcaagaagt ccttcacgag agtcctttat tctgatgata ttattgacga cgtcaacatt
    15481 cttttctttt agccctgtag ttccttgact ttgtttcaga tcatcaataa tgaaaagccg
    15541 gataatattt ccattattgt ccaaaaatgg ataaaagaaa aatccatgta tcggatagga
    15601 cgtaggaagt atagtatgca gattttcggc tgaaacatag atactccatg aagggagttc
    15661 cttgaggatc tgtgcaggaa tgttatctcg actttctggt ttcagaaagc ccttcatcat
    15721 agagaactcc gatgagtgcc aaatgcggga actacgccgc taattatgct taattcttgc
    15781 ttttagcagg cggtgaataa aaaggttgaa attcattttc ttccgggcaa aaatgccatt
    15841 tcacacatta gctttgcagg acgacctgca aacgcttctt tcaccgggga cggccccatt
    15901 gttctggagc ctgatatgtc ctggatcgtt ttattaattg caggtttgct cgaagttgtc
    15961 tgggcgattg gcctgaaata cacccacggt tttacgcgtc ttacgccaag cattatcact
    16021 attgcggcga tgatcgtcag tatcgccatg ctctcttggg caatgcgcac gttgcctgta
    16081 ggaaccgctt atgcggtctg gaccggtatt ggcgctgttg gggcggccat tacagggatt
    16141 ttgctgctgg gtgagtctgc cagcccggca cgtttgctga gccttgggct gatcgttgct
    16201 ggcattattg gtctgaagct gagcactcac taagcgccag gctgtttcac ccaaatcagt
    16261 ttattcactt caaacccttc ccgggtcgcg atggctaaca tttgctgttt catttcatct
    16321 gaaatagtag gggtccgtga aaggatccac aggtagtcgc gatccggacc acaaaccagc
    16381 gcgtgacgat attcccggtc gagtgcaatt acgttatacc cgccatagaa ggggccgaaa
    16441 aaagaaacct taagcgcggc tctgcttggg tcgccggtga aataggcttt cccttccgtt
    16501 ttctgccaca tctccctgtc agggttatag cccttgttaa taacgttgat gccgccgtcg
    16561 tcgcgcaagc tgtatgttgc ggtcacttta tccaatccgc gctcgaaacg atggtcgaag
    16621 cgcgcaattt cataccaggt tcccagatag cgtttggcat caaagttatt taccacggta
    16681 acgcctttcg gcggtgtcgg ggagctacac gcgacaacca ggaatgcagc cgtaactgca
    16741 gcaacgacgg gcaaaatgcg catgggattt tccttgctgt atttttgtta agtgtagatg
    16801 acaacaggaa aaaaagagaa agaaaggagg cccaatatcc tgggcctcat cgtcagttat
    16861 tgcagctttt caagaatgcg ccaggccgcc tcgacacgga cagggttagg atagcttttg
    16921 tttgccagca tcacgatgcc aaggtttttt tctggaacga aggctacgta gctgccaaat
    16981 ccaccagtgg agcccgtttt atgcacccat gaggctttca ctgcgggggc gggcgggttt
    17041 acctcaacgg cgggaagcgc tgccaatgcc actttgctgt cgctgccgtt gatgatcgaa
    17101 tcagctttca gcggccagtt cagcatctcc cagcctaatc cctggtacat atcgccaata
    17161 cgccagtagc gagactgcgc aagcgcaatg ccctgctgga gcgttttctc ctgaacgtgg
    17221 ctggcatcca tgttggcctg aacccagcgg gccatatcaa taacgctgga tttcacgcca
    17281 taggcttcgg cgtcaagttg tcccggagaa ctgtgtacgg gcttcccttc gcgatagccc
    17341 caggcataat ctttttgttc gttctgcgga accgtaatcc aggtatgcgc cagttttaat
    17401 ggttgcagga cgcgtctggt cattgcctct tcgtaactca ttcctgaggg tttcaccgcc
    17461 agcgcgccaa acagaccaat gctggagtta gcgtaaagtc gcttagcgcc cggagtccat
    17521 tgcggctgcc agttttgata aaaatgcagt aatgcggctt tatccctaac gtcatcgggg
    17581 atctgcagcg gtaggccgcc tgccgtatag gtggctaagt gcagcaggcg gataccctgc
    17641 cactgtttgc ctgtcagttc tggccagtat ttcgtgaccg gatcgctgag cttaatttcg
    17701 ccgcgggcga tagcatcgcc gcccaacacg ccgttaaacg tcttactaac cgatcctagc
    17761 tcaaacagcg tttgctgcgt gactgggtgg ttattggcga tatcggcttt accccaggtg
    17821 aaataatagg gttttccctg gtagataacg gcaacggcca tacccggaat agcctgctcc
    17881 tgcatcaacg gggtgatggt gcgattaacg atatcggcaa tctgttgttc tgtttttgcg
    17941 gcagcaaatg tggagaaaga ggctgtcagc agcagagcgc agcataacga ttttttcatc
    18001 atgaaatcag ttccgtaatt aaaagcaaaa aggtgtccgg gcccgtcaga cgcaatcagt
    18061 gtgtttgatt tgcaccgtgt tgacaaacgg ttaaatttag cagcagatat aagtttttcc
    18121 taaattccac gtgtgttttt tattagcttc aaaaatcact atttcacgaa gaatttagac
    18181 tgcttctcac acattgtaac attatttaca accacctttc aatcattttt gataaatcat
    18241 tgatttcatc tttgctgcaa tgatacttaa taaactctgc aagttatcca cagagcaaca
    18301 ctcaatttta ttgatgatat tcttattata ccagacattt ttcatacact cccttgtacg
    18361 gatagttttc cgacaacttc atgattacat atcttgcggt tttgattatt tttgctgcaa
    18421 gaaatacata cttcaaacgg gtaatgactc caacttattg atagtgtttt atgttcagat
    18481 aatgcccgat gactttgtca tgcagctcca ccgattttga gaacgacagc gacttccgtc
    18541 ccagccgtgc caggtgctgc ctcagattca ggttatgccg ctcaattcgc tgcgtatatc
    18601 gcttgctgat tacgtgcagc tttcccttca ggcgggattc atacagcggc cagccatccg
    18661 tcatccatat caccacgtca aagggtgaca gcaggctcat aagacgcccc agtgtcgcca
    18721 tagtgcgttc accgaatacg tgcgcaacaa ccgtcttccg gagcctgtca tacgcgtaaa
    18781 acagccagcg ctggcgcgat ttagccccga cgtatcccca ctgttcgtcc atttccgcgc
    18841 agacgatgac gtcactgccc ggctgtatgc gcgaggttac cgactgcggc ctgagttttt
    18901 taaatggcgg aaaatcgtgt tgaggccaac gcccataatg cgggcggttg cccggcatcc
    18961 aacgccattc atggccatat caatgatttt ctggtgcgta ccgggttgag aagcggtgta
    19021 agtgaactgc agttgccatg ttttacggca gtgagagcag agatagcgct gatgtccggc
    19081 ggtgcttttg ccgttacgca ccaccccgtc agtagctgaa caggagggac agctgataga
    19141 aacagaagcc actggagcac ctcaaaaaca ccatcataca ctaaatcagt aagttggcag
    19201 catcaccgag gatatgatta ccctgtcgac tttcctcgcc tgattcagga aaagcgtaag
    19261 gagtgggagc agattgcagg catacctgta gccgaagtat tttaagataa gctccctcca
    19321 taacagccgg ttcaaatgag ccggcttttg ttcattacaa cagataactt atgccgttac
    19381 cgataaattc gaccaagtgc tttaatcgca gcaattttta tttccatatc atcactttga
    19441 ctcaatctat gtaattcacg agtaatgttc tccgcctgac accggccttc acctaatgca
    19501 tagatcgcag caagtgtaac gtcttttgat ttactcttca tcatttcaaa caagaaagta
    19561 ttagctatcg caggaggcat gctcataatc aatcctcttc atgaaataaa caatggcact
    19621 cacttgcaaa caggagtcca ctatcgcaag cgactcaact tagcaatatc tctcaatttg
    19681 aatgaaaaaa ccagatggca tgtgacaggg aaccgtccgc atgatgtccg tgaccattta
    19741 accccataag ctaaccttgt cacaaacggg cgcagaacag cctgccagac agacaacaaa
    19801 acaaaaatcc aagataaaac atgaataccg gtatctcaat gcgaagaaat aatctccgta
    19861 gaaaccccca tgagaaaaat atcatatggg agaaaatcgc aattatcaca gccagcgcat
    19921 tcattatttt cgctgtgctc tggcattaat tattttcaac acaaaaagga aaaaacaatg
    19981 aatctggtag acgcctttgt caaaaaagta atatccgagc catatgaaga atatggaaaa
    20041 tggtggatag atgttgaata catttcatgg ggtgttcccg ggaaaacaag actgatgttt
    20101 gagtcaaagg agcaggcact ggaagttaaa gaagggtaca aatttttaac gtgagatctc
    20161 tttttgttaa agggcaggaa gcggtaactc cctgccctta gttacgtttc caggcttatc
    20221 agtcgataag ttcccggatc agttccagaa cagcaatcag cgcgtttgca acggcaacta
    20281 ctgccctgag aacgatgatt aacagattaa tcatccgctt ctccatcact ggagaaagaa
    20341 ccccgttgtt cgctcttaca ctgcctgtca caacggtttc tatagtgtca gttatagaca
    20401 gttcttctcc gaccagagca ccaaaaccag caccacctga tgttttggcc aggacttgca
    20461 acctgttctt cccgctgaac aatatgtcgg acagaacatg cctcagagct gacatgccga
    20521 gtatacactt atccagtact tgcagaaata taattccaga cgtactatct acaagtcagt
    20581 tatagacagt tcttttccga ccagagccac ccactctgta acggaacagt aaaaaaaccg
    20641 acctcaccag tcggtttttt tacatctgtt gtttctcaaa atccgcccga catgcccgtc
    20701 acaccgggga aactcatgct ttgaatcaga accaatcatc tcaactttgc cgtctcctga
    20761 aaaaactggc acattcagtt cgataactac ccataaatac agagagaaaa tccgatgtca
    20821 gaaaagtgga gtaaagaatt cgactcttgc tttcaggtgg ggagcataga agaaattgcc
    20881 gatgctctcg agaaacgcag cggaagcgag gaaaacgcgt tagccatgct ggcaatgaca
    20941 gccttcacac tcatgacccg acgtggtatc tgcgaaatgc agaacgtggc ccccgatggc
    21001 aaaggcattc ggctggagct aatcggcttc agggaaaatg aaacaatacc agatacgctt
    21061 cattgaagaa gaaagggcgc aatgaggtca gccttcgcaa caaagcggcg gcagaaaacc
    21121 tgccgccgtg ttgcccgtta acgtttaact tcgtaggcta acgttgccac aagcactgtg
    21181 tttccctgct ggagccggag tccgcaaagc gaatccctca ccatccagac aaaacacaga
    21241 atcgtcacac agacgacgat taacatcatt aaaaacgttc gctgtggcat catagcccct
    21301 ccattgcctt ccggcggtta tcgggctact ataatgttgt tgaggcatta cagtagagcc
    21361 cttggttgat tgataaaaaa atctccaggg gctttctgct gtataccgtc cacaaaacgc
    21421 tttcggcata cagcctcaag cgcccgcagc tattctaacg actttcgtat ctgtaacaat
    21481 ccttttatta tttgaatgat tcagcgttgt ttttgaagtt aacaaccaac ccaatacatg
    21541 gttctctact tcacaacaac gcatgttatg ctgattttag ttaataaaag aatcccctta
    21601 ggagatggaa atgtcgcagt taaaacaacc tattttcctg aaaaagataa aaaaagtcat
    21661 caacacaatc cctgggcttg aagagcaaat ctttgcatgc cgaaataaaa aacgcagcga
    21721 caatccatta ttatttatcg acagaaaaga tgaagaacgc atactaatga gtcgtctcca
    21781 gagccaacaa aaaaatgaag aactggcttc taaactagaa tctttatttc atggaaacga
    21841 attatcgtca ccacacagta ttttgtgttt tatatactgg cgctatacaa agaaaattta
    21901 tcgcctaagc gaggacatca tcagtgatgt ggctaacacc tatgttgata acctagattc
    21961 tacgtcagta cttcaaaaag cataatcaaa gccttgataa atatgcattc cttcgaaatt
    22021 cagctttcac ccattgggtg aaagaaaagt gctcaaaaat atgttaaatt atcagctttt
    22081 atgactcgat atatggtaaa ataatagtaa gaaaagtagt aaaaaggggt tctaattatg
    22141 attaataaaa ttgatttcaa agctaagaat ctaacatcaa atgcaggtct ttttctgctc
    22201 cttgagaatg caaaaagcaa tgggattttt gattttattg aaaatgacct cgtatttgat
    22261 aatgactcaa caaataaaat caagatgaat catataaaga ccatgctctg cggtcacttc
    22321 attggcattg ataagttaga acgtctaaag ctacttcaaa atgatcccct cgtcaacgag
    22381 tttgatattt ccgtaaaaga acctgaaaca gtgtcacggt ttctaggaaa cttcaacttc
    22441 aagacaaccc aaatgtttag agacattaat tttaaagtct ttaaaaaact gctcactaaa
    22501 agtaaattga catccattac gattgatatt gatagtagtg taattaacgt agaaggtcat
    22561 caagaaggtg cgtcaaaagg atataatcct aagaaactgg gaaaccgatg ctacaatatc
    22621 caatttgcat tttgcgacga attaaaagca tatgttaccg gatttgtaag aagtggcaat
    22681 acttacactg caaacggtgc tgcggaaatg atcaaagaaa ttgttgctaa catcaaatca
    22741 gacgatttag aaattttatt tcgaatggat agtggctact ttgatgaaaa aattatcgaa
    22801 acgatagaat ctcttggatg caaatattta attaaagcca aaagttattc tacactcacc
    22861 tcacaagcaa cgaattcatc aattgtattc gttaaaggag aagaaggtag agaaactaca
    22921 gaactgtata caaaattagt taaatgggaa aaagacagaa gatttgtcgt atctcgcgta
    22981 ctgaaaccag aaaaagaaag agcacaatta tcacttttag aaggttccga atacgactac
    23041 tttttctttg taacaaatac taccttgctt tctgaaaaag tagttatata ctatgaaaag
    23101 cgtggtaatg ctgaaaacta tatcaaagaa gccaaatacg acatggcggt gggtcatctc
    23161 ttgctaaagt cattttgggc gaatgaagcc gtgtttcaaa tgatgatgct ttcatataac
    23221 ctatttttgt tgttcaagtt tgattccttg gactcttcag aatacagaca gcaaataaag
    23281 acctttcgtt tgaagggtga tgctgccaac ttactgattt agtgtatgat ggtgtttttg
    23341 aggtgctcca gtggcttctg tttctatcag ctgtccctcc tgttcagcta ctgacggggt
    23401 ggtgcgtaac ggcaaaagca ccgccggaca tcagcgctat ctctgctctc actgccgtaa
    23461 aacatggcaa ctgcagttca cttacaccgc ttctcaaccc ggtacgcacc agaaaatcat
    23521 tgatatggcc atgaatggcg ttggatgccg ggcaaccgcc cgcattatgg gcgttggcct
    23581 caacacgatt ttccgccatt taaaaaactc aggccgcagt cggtaacctc gcgcatacag
    23641 ccgggcagtg acgtcatcgt ctgcgcggaa atggacgaac agtggggata cgtcggggct
    23701 aaatcgcgcc agcgctggct gttttacgcg tatgacaggc tccggaagac ggttgttgcg
    23761 cacgtattcg gtgaacgcac tatggcgaca ctggggcgtc ttatgagcct gctgtcaccc
    23821 tttgacgtgg tgatatggat gacggatggc tggccgctgt atgaatcccg cctgaaggga
    23881 aagctgcacg taatcagcaa gcgatatacg cagcgaattg agcggcataa cctgaatctg
    23941 aggcagcacc tggcacggct gggacggaag tcgctgtcgt tctcaaaatc ggtggagctg
    24001 catgacaaag tcatcgggca ttatctgaac ataaaacact atcaataagt tggagtcatt
    24061 acctattgac gggttttatt tcaccgccaa acttcacgaa atcaatgaag gcgctatggc
    24121 gacgtaatgc cgatactggt tgaatgtcaa aattagccag aataaaaaag taccctggaa
    24181 aaatagcgaa tattctttca cggtgacagc acctggagtc tgcccgtctt atctttcttg
    24241 ttattaatgg agtccatggt acgacgtttt gctcattcag ccatgagaat aaagattctc
    24301 tgtttttgcc ggctgtatta tattgcgcaa gataccagtt ccggttattc aggtgttcta
    24361 tattcacgaa gttcaacgct ccgcgcaaaa ccgatccatg atggacgatt ttgcgatgtc
    24421 aggcgaatat gctaaatcat atgtctgaag cagtgtccag cccatatctg gataaactgc
    24481 agttctgaca ggcatataac cagcagactc tagtacgttc tgtatcggtg atttggcgaa
    24541 ctcacagata aagaacggct aagcactaat atcagcaata cagtggcaca gtatcgtgat
    24601 acgcgaaata aaacatcagc acgtcagttc agagtaaggt cgccatatat cgggcatgag
    24661 ccagatacaa ataattggga caccgtctga agtgatttgc tttattaaag taacttgtgc
    24721 ttggtgttta tgtttatcaa tttgggagcg cctgtaaagt gcaaaatgca atttccatga
    24781 aagtgtcccc tttaatgggg acacctgttc acattttttc gttttcttta gctgtattgc
    24841 taatcatgaa aacgaacgtt cgcatcagca cagcagagcg aagtgcatca tatccttcca
    24901 gatttaagtt cttcaggctt cgcttcaggt aggttcgtac cgtttcggga gaaagccgac
    24961 agttctgagc aatggcgtcg taaggaacac ccattgcgta aaaaacgcag actttaagct
    25021 ggtttgttga tagctcggga aacatgcctt ccaacagagt gatggccggg agtttctgta
    25081 tcatttgagg tgacatagca aaggtctccg atgacaacaa aaaacgctat caccgagttc
    25141 ctacgctcat tggtgatagc ccagacgggg gtaggaatac cggcgtcatc ggaaaccggc
    25201 caggctaatg cctgccccgc ctgagctacc atagatgacg acaagaaata caagttcctg
    25261 tctttatggt atggcaaaaa acgacgccaa atgaagaaat taacttctgc gccgacgatg
    25321 actacgcggg ttcctacgcc cgatcacagt tttgcctgtg accagtaaag ggtacttagt
    25381 ataacgtacc gagtcaacta accgcttaaa aaacatacaa aaaagcgtca aaacttaacg
    25441 ctccgcgccg aagcgatcca ggcatgatgc ttctgctttg tggcgttcga acatgctggt
    25501 ccatatgcac gaaacggtgt ctgaccctcg tccagacaat ccgcagttct gacgaacata
    25561 tgaacaaata tgctcttgta gaggctgttg ttacacaatt ggcgggctgt gtaacaaatg
    25621 gttttattat ctaagaatca ttctatttac gatcgaatga tctacaaaat gattttttaa
    25681 aggtttttta gatcgtttaa ttgtcttaaa acagaatatt attctgttag gttgatcggt
    25741 tgtaagataa atgatcgtga gtagaacaaa agtcgatctt ttaatccaaa agaaggatct
    25801 aaagcagaaa aatctgcttt taaaaataga ttttattttt ttgacgcagg tcaagattaa
    25861 ctcattagag gtatcggtga ggaggcactg gaagagaaga gatcgttgta atgcttttca
    25921 aattaacgta aagcgggtat atttcggttg ttattagctg cgcagagggt ggcactctgt
    25981 ggagcaaagc ggcgaaagcc ggacggcaga atgcgccata aggcattcag gagagatggc
    26041 atttacgggc agtaagtcag aagaccgaag atgttccgga agccataaaa ggaaaacccc
    26101 cacaatcttt cgacgaactt ggcgggacgg agaaagatta tgggggcctc acagaatacg
    26161 ggtaaagtat aatgaaaccg taccagagat tcaaccctgt gcagtgtata aatacacggc
    26221 acaatcgctc cgccataagc gacagctt
//
