LOCUS       NZ_CP135282             2067 bp    DNA     circular CON 07-OCT-2023
DEFINITION  Enterobacter hormaechei strain OHT403-4 plasmid pOHT403-4_6,
            complete sequence.
ACCESSION   NZ_CP135282
VERSION     NZ_CP135282.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37300945
            Assembly: GCF_032298955.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Wittum,T.
  TITLE     Salmonella and antimicrobial resistant bacteria recovered from
            retail pet treats in the US
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Li,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-SEP-2023) CVM/OAS, FDA-CVM, 8401 Muirkirk Rd, MOD2,
            Laurel, MD 20708, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-SEP-2023
            Assembly Method                   :: HGAP v. 4
            Assembly Name                     :: Microbial assembly
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 604x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/07/2023 07:13:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,027
            CDSs (total)                      :: 4,906
            Genes (coding)                    :: 4,800
            CDSs (with protein)               :: 4,800
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 106
            CDSs (without protein)            :: 106
            Pseudo Genes (ambiguous residues) :: 0 of 106
            Pseudo Genes (frameshifted)       :: 39 of 106
            Pseudo Genes (incomplete)         :: 76 of 106
            Pseudo Genes (internal stop)      :: 33 of 106
            Pseudo Genes (multiple problems)  :: 32 of 106
            Pseudo Genes (short protein)      :: 1 of 106
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP135282.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="OHT403-4"
                     /isolation_source="Pig ear pet treat"
                     /host="Food"
                     /db_xref="taxon:158836"
                     /plasmid="pOHT403-4_6"
                     /geo_loc_name="USA:OH"
                     /collection_date="2021-11-08"
                     /collected_by="The Ohio State University"
     gene            784..1749
                     /locus_tag="RRJ53_RS25130"
                     /old_locus_tag="RRJ53_25130"
     CDS             784..1749
                     /locus_tag="RRJ53_RS25130"
                     /old_locus_tag="RRJ53_25130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001287824.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     gene            1753..2019
                     /locus_tag="RRJ53_RS25135"
                     /old_locus_tag="RRJ53_25135"
     CDS             1753..2019
                     /locus_tag="RRJ53_RS25135"
                     /old_locus_tag="RRJ53_25135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024536.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     Typing          1178..1409
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 atgcaatctc tagtgctctg tctatcctgc attatcctca gcattatcct cagcattatc
       61 ctcagcatta tcctcagcct tgccaactcg acaccaatgc aggatagaca atccgatgtc
      121 aaatgttaac actctgcgag tggtacattt tccccggatt atcgtcctga gcctgccgct
      181 ggctctcttt ctaccgcctc gctttgctcg ttgctcaacg cctcacagac acggattaaa
      241 atccgcatcc gttcaccgtt ttttaaagtc cgttaaaagc atgatgccat ctccgagagt
      301 taatctcgtc aaatgctaaa tcgtgggggt cccctttggg gttccgattt agtgattgac
      361 gacaccaccg attaaaaaac ttatgcgggg tggatggttt cacgaagtga ggccatccac
      421 ctgtaagaca gggttttgtt tttattccct gttttggtga tcgggtgtgt ggaaaaggtt
      481 ggggtaagcc gttcgggggt gcttgttttg gggggtattt atacttaagg gataaatggc
      541 ggatatgaaa tagtggttta gcccagtaat gacgaggctt tgagtgggtt ttgacaggtc
      601 aaagaaaatg gagcagaatt gaggcgtttt taatcggcgt tggggagtgc gtcaacactc
      661 cccaacattt cgaatgtgtc acctcagcgg caaactctgg tgacatgtac tggctcgcaa
      721 tgcacaggta cgtgatgaat ataccacatc aaatcacagc ctgcccagat cggagcaggc
      781 ttaatgtcag aagataaatt cctttcggac tacagccccc gtgatgcagt ttgggatacc
      841 cagcgcacgc ttaccgattc tgtcgggggt atctaccaga ctgctgctga attcgagcgc
      901 tatgcactcc gtatggcctc ctgtagcggt ttgttacgtt ttggttggtc taccatcatg
      961 gaaaccggag aaacgcgcct acggcttcgt agtgcgcaat tttgccgtgt ccgtcattgc
     1021 cctgtctgcc agtggagaag aaccctcatg tggcaagccc gtttttatca ggctctaccg
     1081 aaaatcgttg tggattaccc gtcttcccga tggttgtttc tgacgttaac tgtcaggaac
     1141 tgcgagatag gtgaacttgg aacagtcctt acagcaatga atgcggcgtt taagcgaatg
     1201 gaaaagcgaa aggagctatc acctgttcag gggtggatca gggctacgga ggtgacgcga
     1261 ggtaaggatg gcagcgcaca tccgcatttt cactgtctgc tgatggtgca accttcttgg
     1321 tttaaaggga agaactacgt taagcacgaa cgttgggtag aactctggcg cgattgcttg
     1381 cgggtgaact atgagccgaa tatcgatatt cgggcagtaa aaactaagac aggtgaggtt
     1441 gtggccaacg ttgccgagca actgcaaagc gcggttgctg aaacgctgaa atactccgtt
     1501 aaaccggaag atatggcaaa cgatcctgag tggtttcttg agctgacgcg gcagcttcac
     1561 aagcgccgtt ttatctcgac cggtggggcg ctaaaaaacg tcctccagtt ggatcgagaa
     1621 accaatgagg atcttgtcat tgccgacgat gtaggggatg gcactgatga cgggaagcgg
     1681 acggcgtttg tctgggattc aggtaaacgg cgttacaaac gcgcccctga gaaggataaa
     1741 tcggattaac gtatgaatat taatattgaa tacctgaatg gaaataagac tattggttta
     1801 ttttttttaa gaagtgaagc ggtgattcct gacaggttta aaaaccttat tttgcttatt
     1861 gatggattaa gttttggcac atttggtttt catccgcacg aaggttttga ggatgaatta
     1921 attttatata ttcagaaaac aaacgagagg gtaaaaactc tttttgtgaa aatagattta
     1981 aacggtatca agtttggatt tttaagaacg cattcttagt tctggaaaag agccagcggc
     2041 aggctgaggt gataggtacg agattgc
//
