LOCUS       NZ_CP135274            12725 bp    DNA     circular CON 07-OCT-2023
DEFINITION  Enterobacter hormaechei strain IMT49658-1 plasmid unnamed4, complete
            sequence.
ACCESSION   NZ_CP135274
VERSION     NZ_CP135274.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37527804
            Assembly: GCF_032302155.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 12725)
  AUTHORS   Kupke,J., Brombach,J., Wolf,S.A., Thrukonda,L., Ghazisaeedi,F.,
            Kuropka,B., Hanke,D., Semmler,T., Nordholt,N., Schreiber,F.,
            Tedin,K., Luebke-Becker,A., Steiner,U.K. and Fulde,M.
  TITLE     Heteroresistance in Enterobacter cloacae complex caused by
            cell-to-cell variation in transient gene duplication-amplification
            (GDA)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12725)
  AUTHORS   Kupke,J., Fulde,M., Semmler,T. and Wolf,S.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2023) Institute of Microbiology and Epizootics,
            Freie Universitaet Berlin, Robert-von-Ostertag-Str. 7, Berlin 14163,
            Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.9.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/07/2023 07:45:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,084
            CDSs (total)                      :: 4,965
            Genes (coding)                    :: 4,893
            CDSs (with protein)               :: 4,893
            Genes (RNA)                       :: 119
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 24 of 72
            Pseudo Genes (incomplete)         :: 47 of 72
            Pseudo Genes (internal stop)      :: 11 of 72
            Pseudo Genes (multiple problems)  :: 10 of 72
            Pseudo Genes (short protein)      :: 1 of 72
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP135274.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12725
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="IMT49658-1"
                     /host="horse"
                     /db_xref="taxon:158836"
                     /plasmid="unnamed4"
                     /geo_loc_name="Germany"
                     /collection_date="2020"
     gene            111..1436
                     /locus_tag="RP424_RS25325"
     CDS             111..1436
                     /locus_tag="RP424_RS25325"
                     /inference="COORDINATES: protein motif:HMM:NF013211.3"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0140911 - pore-forming activity [Evidence
                     IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA];
                     GO:0050829 - defense response to Gram-negative bacterium
                     [Evidence IEA]; GO:0031640 - killing of cells of another
                     organism [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /protein_id="WP_096889074.1"
                     /translation="MPGFNYGGNSVGDGTNWSSERGSDPTTPGGGDNGHAGDHSSSGSS
                     SSTVEHTPWGDLTTDAAGYAYMNGIKLTADNSQLAYVSPTHLVRVLNSLLDRSYMKPEP
                     GEHSGVTGVPATSPSRVTLADNALKNAQSKAQDAAAALSKANAAISQAQTRLNTAKAKI
                     PSLEANVNTKQQASDKATARAHELAHFLNDMGGPGRYNALNAGYQSEVAQSELDNAKKA
                     LANGHSEITAAQKALDDAKKALPNATANKQKADSAVASAADMKDAITTTTAFFESISKK
                     FGERAARLAQELAAQARSAKIRSVPEAVALFDKHKGTAVNKLNAADKAAIANAFKSLDQ
                     AAMAKQLAAYSKVLGHFNKAMDFADLGAAVKKGIETGNWNDAINKLESIALTNTAGQLA
                     AIAFAAAGAPLGIVGFGIVMTLSGVLFSNEKVLSAINTSLGM"
                     /name="colicin-like pore-forming protein"
     gene            complement(1456..1809)
                     /locus_tag="RP424_RS25330"
     CDS             complement(1456..1809)
                     /locus_tag="RP424_RS25330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071992646.1"
                     /translation="MTIKYYLKHCLWGLIGCGYLTYLILTDLNSGDIYPAYAPYMPYIA
                     SYLAVSAVIFPFAFYTTEQLSLKKMKRETWGNYFGSDSPSWGAFIFVYIFCVLLSVPLF
                     FIYPFINKNRPGN"
                     /name="hypothetical protein"
     gene            1934..2083
                     /locus_tag="RP424_RS25335"
     CDS             1934..2083
                     /locus_tag="RP424_RS25335"
                     /inference="COORDINATES: protein motif:HMM:NF014457.3"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_071992647.1"
                     /translation="MRISFFSGIVIVSMILAACQVNNVRDTGGGTVSPTSTVTGVTLDS
                     GTNR"
                     /name="colicin release lysis protein"
     gene            complement(2286..2420)
                     /locus_tag="RP424_RS25340"
     CDS             complement(2286..2420)
                     /locus_tag="RP424_RS25340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048230020.1"
                     /translation="MHEPPVQSDCCACSGNYRLESNPERHEKTPLAAATGKQVVREMY"
                     /name="hypothetical protein"
     gene            3067..3390
                     /locus_tag="RP424_RS25345"
     CDS             3067..3390
                     /locus_tag="RP424_RS25345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_180241484.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARTGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMTIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3380..4054
                     /locus_tag="RP424_RS25350"
     CDS             3380..4054
                     /locus_tag="RP424_RS25350"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_315451647.1"
                     /translation="MIVKFHPRGRGGGAGPVDYLLGKDRQRDGATVLQGKPEEVRELID
                     ASPYVKKYTSGVLSFAEADLPPGQREKLMASFERVLMPGLDKDQYSILWVEHEDKGRLE
                     LNFLIPNTELLTGRRLQPYYDRADRPRIDAWQTIVNGRLGLHDPNAPENRRALVTPSGL
                     PKTKQEAAEAITRGLLTLASSGELKSRQDVTEALERSGFEVVRTTKSSISIADPDGGET
                     SD"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            4067..4558
                     /locus_tag="RP424_RS25355"
     CDS             4067..4558
                     /locus_tag="RP424_RS25355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_015060400.1"
                     /translation="MSSLLTLAKDLEQQSKAQKQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWLTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            4565..4780
                     /locus_tag="RP424_RS25360"
     CDS             4565..4780
                     /locus_tag="RP424_RS25360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137422.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(4900..5322)
                     /gene="tolA"
                     /locus_tag="RP424_RS25365"
     CDS             complement(4900..5322)
                     /gene="tolA"
                     /locus_tag="RP424_RS25365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014063821.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_023316030.1"
                     /translation="MPLSVRFSPFMFFIPLFLTACTASPHTSVEKSAKAETTQPVDDLL
                     GDLKAGSSDNVASYATKIKHAVEEKMYKPESYKEQKCTIRLLLGRDGLVMGATAESGDS
                     ELCNAVMMAVKQANIPPAPDEKTWQEFRNTSLDFSY"
                     /name="tolA"
     gene            complement(5728..6186)
                     /locus_tag="RP424_RS25370"
     CDS             complement(5728..6186)
                     /locus_tag="RP424_RS25370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005119689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032635016.1"
                     /translation="MAWHTVKDTLKLTGRSRSQLYRDMKKGLVSYRTGKDGRREFDTSE
                     LIRAYGELRTEETVKRHKKGQQEIDDTPAEHPQLKEIQQQLTALQQTVTLMLEDKSTRE
                     AELRQHDEERTQLLAEISRLNEDLEQEKKRSLWSRLFRRDIEEKNREK"
                     /name="hypothetical protein"
     gene            6473..7798
                     /locus_tag="RP424_RS25375"
     CDS             6473..7798
                     /locus_tag="RP424_RS25375"
                     /inference="COORDINATES: protein motif:HMM:NF013211.3"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0140911 - pore-forming activity [Evidence
                     IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA];
                     GO:0050829 - defense response to Gram-negative bacterium
                     [Evidence IEA]; GO:0031640 - killing of cells of another
                     organism [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /protein_id="WP_096889074.1"
                     /translation="MPGFNYGGNSVGDGTNWSSERGSDPTTPGGGDNGHAGDHSSSGSS
                     SSTVEHTPWGDLTTDAAGYAYMNGIKLTADNSQLAYVSPTHLVRVLNSLLDRSYMKPEP
                     GEHSGVTGVPATSPSRVTLADNALKNAQSKAQDAAAALSKANAAISQAQTRLNTAKAKI
                     PSLEANVNTKQQASDKATARAHELAHFLNDMGGPGRYNALNAGYQSEVAQSELDNAKKA
                     LANGHSEITAAQKALDDAKKALPNATANKQKADSAVASAADMKDAITTTTAFFESISKK
                     FGERAARLAQELAAQARSAKIRSVPEAVALFDKHKGTAVNKLNAADKAAIANAFKSLDQ
                     AAMAKQLAAYSKVLGHFNKAMDFADLGAAVKKGIETGNWNDAINKLESIALTNTAGQLA
                     AIAFAAAGAPLGIVGFGIVMTLSGVLFSNEKVLSAINTSLGM"
                     /name="colicin-like pore-forming protein"
     gene            complement(7818..8171)
                     /locus_tag="RP424_RS25380"
     CDS             complement(7818..8171)
                     /locus_tag="RP424_RS25380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071992646.1"
                     /translation="MTIKYYLKHCLWGLIGCGYLTYLILTDLNSGDIYPAYAPYMPYIA
                     SYLAVSAVIFPFAFYTTEQLSLKKMKRETWGNYFGSDSPSWGAFIFVYIFCVLLSVPLF
                     FIYPFINKNRPGN"
                     /name="hypothetical protein"
     gene            8297..8446
                     /locus_tag="RP424_RS25385"
     CDS             8297..8446
                     /locus_tag="RP424_RS25385"
                     /inference="COORDINATES: protein motif:HMM:NF014457.3"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_071992647.1"
                     /translation="MRISFFSGIVIVSMILAACQVNNVRDTGGGTVSPTSTVTGVTLDS
                     GTNR"
                     /name="colicin release lysis protein"
     gene            complement(8649..8783)
                     /locus_tag="RP424_RS25390"
     CDS             complement(8649..8783)
                     /locus_tag="RP424_RS25390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048230020.1"
                     /translation="MHEPPVQSDCCACSGNYRLESNPERHEKTPLAAATGKQVVREMY"
                     /name="hypothetical protein"
     gene            9431..9754
                     /locus_tag="RP424_RS25395"
     CDS             9431..9754
                     /locus_tag="RP424_RS25395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_180241484.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARTGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMTIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            9963..>10397
                     /locus_tag="RP424_RS25400"
                     /pseudo=""
     CDS             9963..>10397
                     /locus_tag="RP424_RS25400"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            10432..10923
                     /locus_tag="RP424_RS25405"
     CDS             10432..10923
                     /locus_tag="RP424_RS25405"
                     /inference="COORDINATES: protein motif:HMM:NF016710.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_015060400.1"
                     /translation="MSSLLTLAKDLEQQSKAQKQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWLTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            10930..11145
                     /locus_tag="RP424_RS25410"
     CDS             10930..11145
                     /locus_tag="RP424_RS25410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137422.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(11265..11687)
                     /gene="tolA"
                     /locus_tag="RP424_RS25415"
     CDS             complement(11265..11687)
                     /gene="tolA"
                     /locus_tag="RP424_RS25415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014063821.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_023316030.1"
                     /translation="MPLSVRFSPFMFFIPLFLTACTASPHTSVEKSAKAETTQPVDDLL
                     GDLKAGSSDNVASYATKIKHAVEEKMYKPESYKEQKCTIRLLLGRDGLVMGATAESGDS
                     ELCNAVMMAVKQANIPPAPDEKTWQEFRNTSLDFSY"
                     /name="tolA"
     gene            complement(12093..12549)
                     /locus_tag="RP424_RS25420"
                     /pseudo=""
     CDS             complement(12093..12549)
                     /locus_tag="RP424_RS25420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005119689.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /name="entry exclusion protein 1"
     Typing          2783..2955
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="89.773"
                     /coverage="100.0"
                     /evalue="9.61e-56"
     Typing          9147..9319
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="89.773"
                     /coverage="100.0"
                     /evalue="9.61e-56"
     Typing          complement(2294..2816)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.656"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(8657..9180)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.667"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          3068..3384
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.453"
                     /coverage="99.0"
                     /evalue="7.36e-61"
     Typing          4016..4872
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.203"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          9432..9748
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.453"
                     /coverage="99.0"
                     /evalue="7.36e-61"
     Typing          9745..11237
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.6"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tatacagtat tatggtgtgg aagagtactt ataacagcgc gctgcggaaa cggcaggcaa
       61 caaaccgcac acctgatttc taataaaaat aaaacaaaaa gggaaaaatt atgcctgggt
      121 ttaattatgg tggtaacagt gtaggggatg gtactaattg gagttctgag cggggttctg
      181 accccactac gccagggggc ggagataatg gtcatgctgg agatcattct tcatctggta
      241 gttcgtcatc aactgttgaa catacccctt ggggagacct gacaaccgac gctgccggct
      301 atgcttatat gaatggtatt aaactgacgg cagataactc gcaacttgct tacgtttctc
      361 caactcatct tgtgcgagta cttaattctc tgctcgatcg ttcttacatg aaacccgagc
      421 caggtgaaca ttccggtgtg acgggggtgc ctgcgacatc cccaagccgt gtaaccctcg
      481 cagataacgc gctgaagaat gcacaatcaa aagcccaaga tgccgcagct gctctgtcga
      541 aagcaaatgc ggctattagt caggcgcaaa cacgtctgaa tacagcaaaa gcgaaaatcc
      601 ctagtcttga agccaatgtt aacacgaagc aacaggcttc agataaggcc actgctcgtg
      661 cgcatgagct ggctcatttc ctgaatgata tgggtgggcc cgggcgttat aatgccttga
      721 atgccggtta tcagtctgaa gttgcacaga gtgagctcga taacgctaaa aaggcgttag
      781 caaatggcca ttctgaaatt accgctgcac agaaagcttt ggatgatgct aaaaaggctt
      841 tacctaatgc cacagcaaac aaacagaagg ctgacagtgc tgtggcatca gctgctgata
      901 tgaaagatgc gattaccact actacagcct ttttcgagtc aatttcgaaa aaattcggtg
      961 aacgggctgc tcgccttgcg caggaactgg ctgcccaggc tcgtagtgca aaaatccgta
     1021 gtgtgcctga ggcggttgct ttgtttgata aacataaggg tactgctgta aacaaactga
     1081 acgcagcaga taaggcggcg atcgcgaatg cgtttaaatc tctcgatcag gcagccatgg
     1141 caaaacaatt ggccgcgtat tcaaaggttt tagggcactt taataaggcg atggattttg
     1201 ctgatttagg tgcggcggta aaaaaaggta ttgagacagg taattggaac gatgccatca
     1261 acaagcttga atctattgct ttaactaata cggctggaca gttagctgcg attgcttttg
     1321 cggcagcagg tgcgccactt gggattgtgg gctttggtat tgtcatgacc ctgtctggtg
     1381 tcttatttag taacgaaaaa gttttgagtg caattaatac atcgttaggc atgtagccag
     1441 tttccgtccg gggtgctagt tacccggacg gtttttatta atgaaaggat aaataaaaaa
     1501 cagtggaact gataacagta cgcaaaaaat ataaacaaaa atgaaagcac cccaagatgg
     1561 gctgtctgaa ccgaaataat taccccatgt ttctcttttc atttttttta atgataattg
     1621 ttctgtggtg taaaaagcaa aagggaagat gactgcactt accgctaagt agctagctat
     1681 gtaaggcata taaggcgcat aagctggata aatgtctcca ctatttaaat ctgtcaagat
     1741 taagtaagta agatatccgc agccaatcaa accccagaga caatgtttta agtaatattt
     1801 aatagtcatt tttttgttta agttgaaatg cattcttgat tttatctaaa aaactgtctt
     1861 tggacaatgg atgaattgta agaccgaaag ttattataaa tgtagcctaa aataataaaa
     1921 agaaggatag aatatgcgaa tatctttttt ctcgggaatc gtcatagtat cgatgattct
     1981 cgcagcatgc caagtgaata acgtccgaga tactggaggt ggtacagttt ctccaacatc
     2041 cacagtaacg ggcgttacgc tcgacagtgg tacgaatagg taacgatcca taaaggatct
     2101 gcttgagatc ttttgcgatg agtcgtaatc tcttgctctg taaacgaaaa aaccacccgg
     2161 caaggtggtt tttcgaagga tcagaaaccg gcaagtttct gaaccaggta acagtcttgt
     2221 gcgagacatg tcaccaaatc tgtcctttca gtgtagccgc agtctggcca tcacttcaag
     2281 tactctcaat acatctctcg cacaacctgc ttaccagtgg ctgctgccag tggcgttttt
     2341 tcgtgtcttt ccgggttgga ctcaagtcga tagttaccgg aacaggcgca gcagtcggac
     2401 tgaaccgggg gttcgtgcat acagtccagc ttggagcgaa ctgccttccc ggaaccgagt
     2461 gtcaggcgtg gaatgagata aacgcggcca taacagcgga atgacaccgg taaaccgaaa
     2521 ggcaggaaca ggagagcgca cgagggagct accaggggga aacgcctggg atctttatag
     2581 tcctgtcggg tttcgccacc actgatttga gcgtcagatt ctgtgatgct tgtcaggggg
     2641 gcggagccta tggaaaaacg gctccgccgt ggccttctcg cttccctgtt aagtttcttc
     2701 ctggcatctt ccaggaaatt tccgccccgt tcgtaagcca tttccgctcg ccgcagccga
     2761 acgaccgagc gtagcgagtc agtgagcgag gaagcggaat atatcctgta tcacatattc
     2821 tgctgacgcg ctggtgccac ctttttctcc tgctacatga agcactttca taaagtgtta
     2881 ctccgccgta acatattaag ccagtataca ctccgctagc gctacgtgac tggttcaggg
     2941 ctgcgccccg aaacccgcca aaagcctgac gcgcctgcgg cttgtccaac cccggccgac
     3001 cgggaaaggt tttcccgccc gtcccggcgt tatcaggaga ccggtttgtc agagaaacgg
     3061 aacaagatgc tcaccatgtg ggtgacggag gacgagcacc ggcggctgct tgagcgctgc
     3121 gacggcaggc agctggcagc gtggatgcgg cagacctgcc tggacgaaaa gcctgcgcgt
     3181 accggaaagc ttccctcgat ctcgccggcg ctgctgcgcc agcttgccgg catgggcaac
     3241 aacctgaacc agatagcccg ccgcgttaac gccggcggtg gcactggcca tgacagggtg
     3301 cagattgtgg cggcgctgat gaccatcgat gccggacttg agcggctgcg gcacgccgtt
     3361 cttgagaaag gaacggacga tgatcgttaa atttcatcca cgcggtcgcg gcggtggcgc
     3421 cgggccggtg gactacctgc tgggtaaaga ccggcagcgt gatggtgcca ccgtcctgca
     3481 ggggaagccg gaagaagtcc gggagctgat tgacgcgtcg ccttacgtga agaaatacac
     3541 ttccggcgtc ctgtcgtttg cggaggccga tctgcctccc ggccagcggg aaaaactgat
     3601 ggccagcttc gagcgggttc tgatgcccgg acttgataaa gatcagtaca gcattctgtg
     3661 ggtggagcac gaggacaagg ggcgtctgga gctgaacttt ctgatcccca ataccgagct
     3721 gctgaccggc aggcggctcc agccgtacta cgaccgcgcc gaccggccgc gcatcgatgc
     3781 ctggcagacc atcgtgaacg gcagactggg gctgcacgac ccgaacgcgc cggaaaaccg
     3841 ccgggcgctg gtcacgccgt ccggacttcc gaaaacgaag caggaggccg cagaagccat
     3901 cacgcgcgga ctgctgaccc ttgcttcgtc cggggagctg aaaagccgtc aggacgtcac
     3961 tgaggcgctg gaacggtcag gttttgaggt ggtgcgcacc acgaaaagca gtatcagcat
     4021 tgccgacccg gacgggggcg aaacatccga ctgaagggag ccatctatga gcagtctttt
     4081 aacgctggcg aaggacttag agcagcaatc gaaagcgcag aagcagagta ccggcgagat
     4141 gctgaaagcc gcattcagcg agcacgagca gtctgtcaga gcggaactga gcgcaagcgc
     4201 gaggagaatc agcgacgcca tcctcgccca cgagcagagt atgagcgagg cgatggagaa
     4261 aaaccggcgg agcgtactgc ggactgccgg gcggacatgg ctcaccatcc tgatggtgtc
     4321 agcgctgctg atcggcacga gcgggagcat tctgtggtgg caggggcagc agataaccga
     4381 caattacacg catcttcgtc agcaggaaga taccctggcg aaaatgaccg cccggacgtg
     4441 gggagtgcgc tatcaggaga gcagcgacgg ccggaggttt cttatcctgc cgccggggat
     4501 gcaaaccgaa gccatccctt acgacgggac gacatggatc cgcctgaaac aggagtagcg
     4561 gaagatgaca gagctggaaa agcagttgct gagcgcatta gagcagctac agcaggacta
     4621 ctcgcaaagg ctggacgaat gggagagcgc cttcgcggaa tggcggagca tgtgtgggct
     4681 tatgcaacgg gagaacgcgg tgctgaacga gcgcgtgtcg gacttgagca cgcaggtgct
     4741 gagtttaagc gagcagctgc gccgcttgtc cgggaactga atgccatcga gcgccggcag
     4801 agagataatc agtatcagaa aacactggag ctggagcggc agccgcagaa aacctaccgg
     4861 ggaccgacac tgtgactgac ctccagcagg ggaaatcttt cagtatgaaa aatctagaga
     4921 ggtatttctg aattcctgcc aagttttttc atccggtgca ggaggaatat tcgcttgttt
     4981 aacagccatc attactgcgt tacaaagctc tgagtccccg ctctccgctg ttgcgcccat
     5041 aactaaacca tcgcgcccga gtaagagccg aatagtacat ttctgctcct tatatgattc
     5101 aggtttgtac atcttttctt cgacggcgtg ttttattttt gttgcataac tggcgacatt
     5161 atcgcttgaa cctgctttta aatcccctag caggtcgtca acgggctggg ttgtttctgc
     5221 ttttgccgat ttttctacgc ttgtatgtgg agaggcagtg catgccgtca aaaacagcgg
     5281 gataaagaac ataaatggtg aaaagcgtac tgataaaggc atcgcggcat tccttgttaa
     5341 cataaaggag ccatcataat tctgtggtta aaccaaagta aactatcaga aatcacgctc
     5401 tcgccatccg tttcccgtca gattaagaat catgcatctt tctgcatagt catgcatgcc
     5461 gataaaacct ggcaggtgcg tttctggcgg ttttccgggt ggtttgttgc ctgttttatc
     5521 ccgtagccgc cggaaatgcc ctcaggccgt ctgagcggtg cgcgtaatga gacgttatgg
     5581 taaatcgttt gtcagcagct ggtgacatag caccagagca agtacaacgc cagcataaac
     5641 caaatccccc acttcagaca ccatcgccag tatctgaaca gatgcttctc ccgtgtatcc
     5701 gcttcatgaa tgacgttctc caaactgtca tttttcacga tttttctcct caatatcacg
     5761 tcggaacaac ctggaccaga ggctgcgttt tttttcctgc tccagatctt cattcagtcg
     5821 gctgatttcg gccagcagct gtgtccgctc ctcatcgtgt tgccggagtt cggcttcgcg
     5881 agtgctttta tcctccaaca tcaatgtgac cgtctgctgc agggcagtta actgctgttg
     5941 tatctctttc agttgcgggt gttcagctgg agtatcgtca atttcttgct gtcccttttt
     6001 gtgccttttg actgtctcct ccgtccgcag ttcaccgtaa gcccgaatca gctcgctggt
     6061 gtcgaattct cgccgtccat ctttccctgt gcggtaactg acaaggcctt ttttcatgtc
     6121 ccgatacagc tgggatcgcg aacgaccggt cagttttagt gtgtccttta cggtgtgcca
     6181 agccataaaa attaccgtgt ctcctgtatg tctcatgttg agctcgcaga ttatatttct
     6241 gttttaacat ggcatatcta aggttgtccg caggaagtga aaaaaccaca aaaaaatcat
     6301 gttgacgatg tgcttttttc gggcgtagta ttagatgtac tgtatataaa cacattgtta
     6361 tatatacagt attatggtgt ggaagagtac ttataacagc gcgctgcgga aacggcaggc
     6421 aacaaaccgc acacctgatt tctaataaaa ataaaacaaa aagggaaaaa ttatgcctgg
     6481 gtttaattat ggtggtaaca gtgtagggga tggtactaat tggagttctg agcggggttc
     6541 tgaccccact acgccagggg gcggagataa tggtcatgct ggagatcatt cttcatctgg
     6601 tagttcgtca tcaactgttg aacatacccc ttggggagac ctgacaaccg acgctgccgg
     6661 ctatgcttat atgaatggta ttaaactgac ggcagataac tcgcaacttg cttacgtttc
     6721 tccaactcat cttgtgcgag tacttaattc tctgctcgat cgttcttaca tgaaacccga
     6781 gccaggtgaa cattccggtg tgacgggggt gcctgcgaca tccccaagcc gtgtaaccct
     6841 cgcagataac gcgctgaaga atgcacaatc aaaagcccaa gatgccgcag ctgctctgtc
     6901 gaaagcaaat gcggctatta gtcaggcgca aacacgtctg aatacagcaa aagcgaaaat
     6961 ccctagtctt gaagccaatg ttaacacgaa gcaacaggct tcagataagg ccactgctcg
     7021 tgcgcatgag ctggctcatt tcctgaatga tatgggtggg cccgggcgtt ataatgcctt
     7081 gaatgccggt tatcagtctg aagttgcaca gagtgagctc gataacgcta aaaaggcgtt
     7141 agcaaatggc cattctgaaa ttaccgctgc acagaaagct ttggatgatg ctaaaaaggc
     7201 tttacctaat gccacagcaa acaaacagaa ggctgacagt gctgtggcat cagctgctga
     7261 tatgaaagat gcgattacca ctactacagc ctttttcgag tcaatttcga aaaaattcgg
     7321 tgaacgggct gctcgccttg cgcaggaact ggctgcccag gctcgtagtg caaaaatccg
     7381 tagtgtgcct gaggcggttg ctttgtttga taaacataag ggtactgctg taaacaaact
     7441 gaacgcagca gataaggcgg cgatcgcgaa tgcgtttaaa tctctcgatc aggcagccat
     7501 ggcaaaacaa ttggccgcgt attcaaaggt tttagggcac tttaataagg cgatggattt
     7561 tgctgattta ggtgcggcgg taaaaaaagg tattgagaca ggtaattgga acgatgccat
     7621 caacaagctt gaatctattg ctttaactaa tacggctgga cagttagctg cgattgcttt
     7681 tgcggcagca ggtgcgccac ttgggattgt gggctttggt attgtcatga ccctgtctgg
     7741 tgtcttattt agtaacgaaa aagttttgag tgcaattaat acatcgttag gcatgtagcc
     7801 agtttccgtc cggggtgcta gttacccgga cggtttttat taatgaaagg ataaataaaa
     7861 aacagtggaa ctgataacag tacgcaaaaa atataaacaa aaatgaaagc accccaagat
     7921 gggctgtctg aaccgaaata attaccccat gtttctcttt tcattttttt taatgataat
     7981 tgttctgtgg tgtaaaaagc aaaagggaag atgactgcac ttaccgctaa gtagctagct
     8041 atgtaaggca tataaggcgc ataagctgga taaatgtctc cactatttaa atctgtcaag
     8101 attaagtaag taagatatcc gcagccaatc aaaccccaga gacaatgttt taagtaatat
     8161 ttaatagtca ttttttttgt ttaagttgaa atgcattctt gattttatct aaaaaactgt
     8221 ctttggacaa tggatgaatt gtaagaccga aagttattat aaatgtagcc taaaataata
     8281 aaaagaagga tagaatatgc gaatatcttt tttctcggga atcgtcatag tatcgatgat
     8341 tctcgcagca tgccaagtga ataacgtccg agatactgga ggtggtacag tttctccaac
     8401 atccacagta acgggcgtta cgctcgacag tggtacgaat aggtaacgat ccataaagga
     8461 tctgcttgag atcttttgcg atgagtcgta atctcttgct ctgtaaacga aaaaaccacc
     8521 cggcaaggtg gtttttcgaa ggatcagaaa ccggcaagtt tctgaaccag gtaacagtct
     8581 tgtgcgagac atgtcaccaa atctgtcctt tcagtgtagc cgcagtctgg ccatcacttc
     8641 aagtactctc aatacatctc tcgcacaacc tgcttaccag tggctgctgc cagtggcgtt
     8701 ttttcgtgtc tttccgggtt ggactcaagt cgatagttac cggaacaggc gcagcagtcg
     8761 gactgaacgg ggggttcgtg catacagtcc agcttggagc gaactgcctt cccggaaccg
     8821 agtgtcaggc gtggaatgag ataaacgcgg ccataacagc ggaatgacac cggtaaaccg
     8881 aaaggcagga acaggagagc gcacgaggga gctaccaggg ggaaacgcct gggatcttta
     8941 tagtcctgtc gggtttcgcc accactgatt tgagcgtcag attctgtgat gcttgtcagg
     9001 gggggcggag cctatggaaa aacggctccg ccgtggcctt ctcgcttccc tgttaagttt
     9061 cttcctggca tcttccagga aatttccgcc ccgttcgtaa gccatttccg ctcgccgcag
     9121 ccgaacgacc gagcgtagcg agtcagtgag cgaggaagcg gaatatatcc tgtatcacat
     9181 attctgctga cgcgctggtg ccaccttttt ctcctgctac atgaagcact ttcataaagt
     9241 gttactccgc cgtaacatat taagccagta tacactccgc tagcgctacg tgactggttc
     9301 agggctgcgc cccgaaaccc gccaaaagcc tgacgcgcct gcggcttgtc caaccccggc
     9361 cgaccgggaa aggttttccc gcccgtcccg gcgttatcag gagaccggtt tgtcagagaa
     9421 acggaacaag atgctcacca tgtgggtgac ggaggacgag caccggcggc tgcttgagcg
     9481 ctgcgacggc aggcagctgg cagcgtggat gcggcagacc tgcctggacg aaaagcctgc
     9541 gcgtaccgga aagcttccct cgatctcgcc ggcgctgctg cgccagcttg ccggcatggg
     9601 caacaacctg aaccagatag cccgccgcgt taacgccggc ggtggcactg gccatgacag
     9661 ggtgcagatt gtggcggcgc tgatgaccat cgatgccgga cttgagcggc tgcggcacgc
     9721 cgttcttgag aaaggaacgg acgatgatcg ttaaatttca tccacgcggt cgcggcggtg
     9781 gcgccgggcc ggtggactac ctgctgggta aagaccggca gcgtgatggt gccaccgtcc
     9841 tgcaggggaa gccggaagaa gtccgggagc tgattgacgc gtcgccttac gtgaagaaat
     9901 acacttccgg cgtcctgtcg tttgcggagg ccgatctgcc tcccggccag cgggaaaaac
     9961 tgatggccag cttcgagcgg gttctgatgc ccggacttga taaagatcag tacagcattc
    10021 tgtgggtgga gcacgaggac aaggggcgtc tggagctgaa ctttctgatc cccaataccg
    10081 agctgctgac cggcaggcgg ctccagccgt actacgaccg cgccgaccgg ccgcgcatcg
    10141 atgcctggca gaccatcgtg aacggcagac tggggctgca cgacccgaac gcgccggaaa
    10201 accgccgggc gctggtcacg ccgtccggac ttccgaaaac gaagcaggag gccgcagaag
    10261 ccatcacgcg cggactgctg acccttgctt cgtccgggga gctgaaaagc cgtcaggacg
    10321 tcactgaggc gctggaacgg tcaggttttg aggtggtgcg caccacgaaa agcagtatca
    10381 gcattgccga cccggacggg gggcgaaaca tccgactgaa gggagccatc tatgagcagt
    10441 cttttaacgc tggcgaagga cttagagcag caatcgaaag cgcagaagca gagtaccggc
    10501 gagatgctga aagccgcatt cagcgagcac gagcagtctg tcagagcgga actgagcgca
    10561 agcgcgagga gaatcagcga cgccatcctc gcccacgagc agagtatgag cgaggcgatg
    10621 gagaaaaacc ggcggagcgt actgcggact gccgggcgga catggctcac catcctgatg
    10681 gtgtcagcgc tgctgatcgg cacgagcggg agcattctgt ggtggcaggg gcagcagata
    10741 accgacaatt acacgcatct tcgtcagcag gaagataccc tggcgaaaat gaccgcccgg
    10801 acgtggggag tgcgctatca ggagagcagc gacggccgga ggtttcttat cctgccgccg
    10861 gggatgcaaa ccgaagccat cccttacgac gggacgacat ggatccgcct gaaacaggag
    10921 tagcggaaga tgacagagct ggaaaagcag ttgctgagcg cattagagca gctacagcag
    10981 gactactcgc aaaggctgga cgaatgggag agcgccttcg cggaatggcg gagcatgtgt
    11041 gggcttatgc aacgggagaa cgcggtgctg aacgagcgcg tgtcggactt gagcacgcag
    11101 gtgctgagtt taagcgagca gctgcgccgc ttgtccggga actgaatgcc atcgagcgcc
    11161 ggcagagaga taatcagtat cagaaaacac tggagctgga gcggcagccg cagaaaacct
    11221 accggggacc gacactgtga ctgacctcca gcaggggaaa tctttcagta tgaaaaatct
    11281 agagaggtat ttctgaattc ctgccaagtt ttttcatccg gtgcaggagg aatattcgct
    11341 tgtttaacag ccatcattac tgcgttacaa agctctgagt ccccgctctc cgctgttgcg
    11401 cccataacta aaccatcgcg cccgagtaag agccgaatag tacatttctg ctccttatat
    11461 gattcaggtt tgtacatctt ttcttcgacg gcgtgtttta tttttgttgc ataactggcg
    11521 acattatcgc ttgaacctgc ttttaaatcc cctagcaggt cgtcaacggg ctgggttgtt
    11581 tctgcttttg ccgatttttc tacgcttgta tgtggagagg cagtgcatgc cgtcaaaaac
    11641 agcgggataa agaacataaa tggtgaaaag cgtactgata aaggcatcgc ggcattcctt
    11701 gttaacataa aggagccatc ataattctgt ggttaaacca aagtaaacta tcagaaatca
    11761 cgctctcgcc atccgtttcc cgtcagatta agaatcatgc atctttctgc atagtcatgc
    11821 atgccgataa aacctggcag gtgcgtttct ggcggttttc cgggtggttt gttgcctgtt
    11881 ttatcccgta gccgccggaa atgccctcag gccgtctgag cggtgcgcgt aatgagacgt
    11941 tatggtaaat cgtttgtcag cagctggtga catagcacca gagcaagtac aacgccagca
    12001 taaaccaaat cccccacttc agacaccatc gccagtatct gaacagatgc ttctcccgtg
    12061 tatccgcttc atgaatgacg ttctccaaac tgtcattttt cacgattttt ctcctcaata
    12121 tcacgtcgga acaacctgga ccagaggctg cgttttttcc tgctccagat cttcattcag
    12181 tcggctgatt tcggccagca gctgtgtccg ctcctcatcg tgttgccgga gttcggcttc
    12241 gcgagtgctt ttatcctcca acatcaatgt gaccgtctgc tgcagggcag ttaactgctg
    12301 ttgtatctct ttcagttgcg ggtgttcagc tggagtatcg tcaatttctt gctgtccctt
    12361 tttgtgcctt ttgactgtct cctccgtccg cagttcaccg taagcccgaa tcagctcgct
    12421 ggtgtcgaat tctcgccgtc catctttccc tgtgcggtaa ctgacaaggc cttttttcat
    12481 gtcccgatac agctgggatc gcgaacgacc ggtcagtttt agtgtgtcct ttacggtgtg
    12541 ccaagccata aaaattaccg tgtctcctgt atgtctcatg ttgagctcgc agattatatt
    12601 tctgttttaa catggcatat ctaaggttgt ccgcaggaag tgaaaaaacc acaaaaaaat
    12661 catgttgacg atgtgctttt ttcgggcgta gtattagatg tactgtatat aaacacattg
    12721 ttata
//
