LOCUS       NZ_CP135268             4783 bp    DNA     circular CON 07-OCT-2023
DEFINITION  Enterobacter asburiae strain FAHZZU5722 plasmid unnamed6, complete
            sequence.
ACCESSION   NZ_CP135268
VERSION     NZ_CP135268.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37542530
            Assembly: GCF_032301015.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter asburiae
  ORGANISM  Enterobacter asburiae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4783)
  AUTHORS   Li,C.
  TITLE     Coexistence of blaNDM and mcr-10 in Enterobacter asburiae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4783)
  AUTHORS   Li,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2023) The first affiliated hospital of Zhengzhou
            University, Zhengzhou University, 100 Science Avenue, Zhengzhou,
            Henan 450001, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. v3.10.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/07/2023 07:06:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,003
            CDSs (total)                      :: 4,885
            Genes (coding)                    :: 4,745
            CDSs (with protein)               :: 4,745
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 35 of 140
            Pseudo Genes (incomplete)         :: 97 of 140
            Pseudo Genes (internal stop)      :: 25 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            Pseudo Genes (short protein)      :: 1 of 140
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP135268.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4783
                     /organism="Enterobacter asburiae"
                     /mol_type="genomic DNA"
                     /strain="FAHZZU5722"
                     /host="Homo sapiens"
                     /db_xref="taxon:61645"
                     /plasmid="unnamed6"
                     /geo_loc_name="China"
                     /collection_date="2022-03-04"
     gene            complement(<76..198)
                     /locus_tag="RQP58_RS24980"
                     /old_locus_tag="RQP58_24980"
                     /pseudo=""
     CDS             complement(<76..198)
                     /locus_tag="RQP58_RS24980"
                     /old_locus_tag="RQP58_24980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487656.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            849..1172
                     /locus_tag="RQP58_RS24985"
                     /old_locus_tag="RQP58_24985"
     CDS             849..1172
                     /locus_tag="RQP58_RS24985"
                     /old_locus_tag="RQP58_24985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_163063187.1"
                     /translation="MLTMWVTEGEHRRLLERCDGRPLAAWMRQTCLDEKPARTGKLPSL
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1381..>1815
                     /locus_tag="RQP58_RS24990"
                     /old_locus_tag="RQP58_24990"
                     /pseudo=""
     CDS             1381..>1815
                     /locus_tag="RQP58_RS24990"
                     /old_locus_tag="RQP58_24990"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1850..2341
                     /locus_tag="RQP58_RS24995"
                     /old_locus_tag="RQP58_24995"
     CDS             1850..2341
                     /locus_tag="RQP58_RS24995"
                     /old_locus_tag="RQP58_24995"
                     /inference="COORDINATES: protein motif:HMM:NF016710.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_213757678.1"
                     /translation="MSSLLTLAKDLEQQSKAQKQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAILAYEQSMSEAMEKNRRSVLRTAGRTWLTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            2348..2563
                     /locus_tag="RQP58_RS25000"
                     /old_locus_tag="RQP58_25000"
     CDS             2348..2563
                     /locus_tag="RQP58_RS25000"
                     /old_locus_tag="RQP58_25000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137422.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3234..3476)
                     /locus_tag="RQP58_RS25005"
                     /old_locus_tag="RQP58_25005"
     CDS             complement(3234..3476)
                     /locus_tag="RQP58_RS25005"
                     /old_locus_tag="RQP58_25005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023316026.1"
                     /translation="MALVRSGNKSKIFEADTKFNSVFDTEYKPGETCEYEGIYKCTGCG
                     KEVTIAGGKTIPPQNHHQHTPAQGNIRWQLFVYAQ"
                     /name="hypothetical protein"
     gene            3663..3980
                     /locus_tag="RQP58_RS25010"
                     /old_locus_tag="RQP58_25010"
     CDS             3663..3980
                     /locus_tag="RQP58_RS25010"
                     /old_locus_tag="RQP58_25010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_023316025.1"
                     /translation="MQQDHEHDDCRIQFRDDWLYQFYKYGKANRKIPTAISASLGRKLD
                     MLNAADKVGDLRSPPGNRFENLNPPLTGYSSIRVNEKYRLIFKWGENGVEDLYLDPHDY
                     R"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            4183..4539
                     /locus_tag="RQP58_RS25015"
                     /old_locus_tag="RQP58_25015"
     CDS             4183..4539
                     /locus_tag="RQP58_RS25015"
                     /old_locus_tag="RQP58_25015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075993.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="WP_023316024.1"
                     /translation="MSQATRKPSTVGDIILYEYLEPSGLKINDLAEMLNVHRNSASALV
                     NNNRKLSTDMAFRLAKAFDTTAKFWLNIQQSVDLWEVTSSPRLQEEIDRVIPAHEIIER
                     KHIVDSSEKKHAIA"
                     /name="HigA family addiction module antitoxin"
     Typing          561..734
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.0"
                     /coverage="100.0"
                     /evalue="1.21e-74"
     Typing          complement(86..594)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.51"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          850..1166
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.453"
                     /coverage="99.0"
                     /evalue="1.45e-59"
     Typing          1163..2655
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.4"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aaatactgtc cttccagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
       61 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgttttgtc
      121 gtgtctttcc gggttggact caagtcgata gttaccggaa caggcgcagc ggtcggactg
      181 aacggggggt tcgtgcatac agtccagctt ggagcgaact gcctacccgg aaccgagtgt
      241 caggcgtgga atgagacaaa cgcggccata acagcggaat gacaccggta aaccgaaagg
      301 caggaacagg agagcgcacg agggagccat cagggggaaa cgcctggtat ctttatagtc
      361 ctgtcgggtt tcgccaccac tgatttgagc gtcagattct gtgatgctcg tcaggggggc
      421 ggagcctatg gaaaaccggc tctgccgtgg ccttatcgct tccctgttaa gtttcttcct
      481 ggcatcttcc aggaaatttc cgccccgttc gtaagcccgc accgctcgcc gcagccgaac
      541 gaccgagcgc agcgagtcag tgagcgagga agcggaatat atcctgtatc gcatattctg
      601 ctgacgcgcc gttgccgcct tttttctcct gccacatgaa gcacttcact gatttccgta
      661 tccgtgccaa catattaagc cagtatacac tccgctagcg ctacgtgact ggttcagggc
      721 tgcgccccga aacccgccaa aagccactga cgcgcctgcg gcttgtccaa ccccgcgccg
      781 accgggaaag gctttcccgc ccgtcccggc gttatcagga ggccggtttg tcagagaaac
      841 ggaacaagat gctcaccatg tgggtgacgg agggcgagca ccggcggctg cttgagcgct
      901 gcgacggcag gccgctggcg gcgtggatgc ggcagacctg cctggacgaa aagcctgcgc
      961 gcaccggaaa gctcccctca ctctcgccgg cgctgctgcg ccagcttgcc ggtatgggca
     1021 ataacctgaa ccagatagcc cgccgtgtta acgccggcgg cggcaccggc catgacaggg
     1081 tacagattgt ggcggcgctg atggccatcg atgccgggct tgagcggctg cggcacgccg
     1141 ttcttgagaa aggaacggac gatgatcgtt aaatttcatc cacgcggtcg cggcggtggc
     1201 gccgggccgg tggactatct gctgggtaaa gaccggcagc gtgaaggcgc cagcgtcctg
     1261 caggggaagc cggaagaagt ccgggagctg attgacgcgt cgccttacgt gaagaaatac
     1321 acctccggcg tactgtcgtt tgcggaggcc gatctgcctc ccggccagcg ggaaaaactg
     1381 atggccagct tcgagcgggt tctgatgccc ggactcgata aagatcagta cagcattctg
     1441 tgggtggagc acgcggacaa ggggcgtctg gagctgaact ttctgatccc caataccgaa
     1501 ctgctgaccg gcaggcggct ccagccgtac tacgaccgcg ccgaccggcc gcgcatcgat
     1561 gcctggcaga ccatcgtgaa cggcagactg gggctgcatg acccgaacgc gccggaaaac
     1621 cgccgggcgc tggtcacgcc gtccggactg ccgaaaacga agcaggaggc cactgaagcc
     1681 atcacgcgcg gactgctgac ccttgcttcg tccggggagc tgaaaaaccg tcaggacgtc
     1741 actgaggcgc tggaacggtc aggttttgag gtggtgcgca ccacgaaaag cagtatcagc
     1801 attgccgacc cggacggggg gcgaaacatc cgactgaagg gagccatcta tgagcagtct
     1861 tttaacgctg gcaaaggact tagagcagca atcgaaagcg cagaagcaga gtaccggcga
     1921 gatgctgaaa gccgcattca gcgagcacga gcagtctgtc agagcggaac tgagcgcaag
     1981 cgcgaggaga atcagcgacg ccatcctcgc ctacgagcag agtatgagcg aggcgatgga
     2041 gaaaaaccgg cggagcgtac tgcggactgc cgggcggaca tggctcacca tcctgatggt
     2101 gtcagcgctg ctgatcggca cgagcgggag cattctgtgg tggcaggggc agcagataac
     2161 cgacaattac acgcatcttc gtcagcagga agataccctg gcgaaaatga ccgcccggac
     2221 gtggggagtg cgctatcagg agagcagcga cggcaggagg tttcttatcc tgccgccggg
     2281 gatgcaaacc gaagccatcc cctacgacgg gacgacatgg atccgcctga aacaggagta
     2341 gcggaaaatg acagagctgg aaaagcagtt gctgagcgca ttagagcagc tacagcagga
     2401 ctactcgcaa aggctggacg aatgggagag cgccttcgcg gaatggcgga gcatgtgtgg
     2461 gcttatgcaa cgggagaacg cggtgctgaa cgagcgcgtg tcggacttga gcacgcaggt
     2521 gctgagttta agcgagcagc tgcgccgctt gtccgggaac tgaatgccat cgagcttcgg
     2581 cagagggaga atgagtatca gaaaacactg gagctggagc gccagccgca gaaaacctac
     2641 cgcgggccga cactttaaac catccaggca gctgacaacc cgcacgaaga ggggctctta
     2701 tttctcccgc taaataggtt cgtgatcaaa aaggtcacga cttattcagg agcgggaaat
     2761 gaagctgtta aatcttattg aatcattgct taactgcctg tatctggcgg cggagctgtg
     2821 ggactggctg gagaagcatt tgtcggtttt taaggcattg gcgcggattt tagtgtgact
     2881 ttaacatcca tgcatctggc tgcatgacta tgcaccagtg taatattcag cggctgcgtt
     2941 tctggtgttt tttcgttatg tctgtcgcca ctttctcgcg tctggcaccg gaaacgcagc
     3001 caaccccgtt tcagcggtgc gcccaacgga tgttatggta aatcagtatc agctgcagat
     3061 ataaaaaagc cgccaggctg gcggcttgct gttattttcg tggctttcct gcatctgagc
     3121 gttttcctct gacccttccg tcattgttac ggtcacgagg ttgagttccg ccgcctggtt
     3181 taggaacgac aacgtgtggg ttgagtggtc gacgatctgc cattttctat acctcactga
     3241 gcataaacaa acaattgcca acgaatgttc ccttgcgcgg gggtatgttg gtggtggttt
     3301 tgtggtggga ttgttttccc gcctgcgata gttacttctt taccgcaacc ggtacattta
     3361 taaatccctt catattcaca tgtttctccc ggcttatatt cagtgtcaaa aaccgagtta
     3421 aacttcgtat ctgcttcaaa aatcttgctt ttgtttcctg agcgaacaag agccatgcaa
     3481 cctccaaatt atcatttatt ttgcacacct gaactgtgca tgagtgcatt ctacagcata
     3541 gccattgatt gcacaagtgc actgtgctgt tgtgcatttg agatgtttgc tgtactatat
     3601 cggaggtaag aaggtgatcg aaaaaattgc caagttgcgg caaaaattga ggtaaaagaa
     3661 aaatgcagca agatcatgaa catgatgatt gccgcattca gtttcgagac gattggctat
     3721 accagtttta caaatatggt aaagccaatc gaaagattcc aactgcgatt agtgcttcat
     3781 taggtcgcaa gctggatatg ctgaatgctg ctgataaagt tggagatttg cggtctcccc
     3841 cgggaaaccg atttgagaat ctcaaccctc cattgacagg gtattcatcc atacgggtga
     3901 atgagaagta ccgcttgata tttaagtggg gtgaaaacgg agtcgaggat ttgtatctcg
     3961 atccacatga ttaccgatag ataacaggtc gtggctgtta tcttataaaa caagtaggtg
     4021 aggcgttccc tcacctacat taaaatgtca ttacaaataa tgattaataa aaaaaatgac
     4081 gtgactatat ttaagataat tactcatcgc tacagctggt aatagacgaa atacttatct
     4141 tcctcatcta acagagagaa gtaaaaaatt taggagtaaa gcatgtccca agcaactaga
     4201 aaaccatcaa ctgtagggga tattattctt tatgaatatc tcgaaccaag tggtttaaaa
     4261 atcaatgact tggctgaaat gttgaatgtg catagaaact ctgccagtgc tttagttaac
     4321 aataatagaa agctgtctac cgatatggct tttaggttgg ctaaagcctt cgataccact
     4381 gcaaagttct ggctaaatat acagcagtct gttgatttat gggaggttac tagtagccct
     4441 cgattgcaag aagaaataga cagagtgatt cccgctcatg aaatcataga aaggaaacac
     4501 attgttgata gttctgagaa gaaacatgct attgcatgat tttttttaaa aaaatataag
     4561 ttatccctca aaaagaaggc ttctagttat agaagccttc tttttgaggg ataaaaacgc
     4621 tcatgttaac tttcttctta cgatcttctt gagatccgtg ctctgtacgc gtaatctctt
     4681 gctctgtaaa cgaaaaaacc gcctggggag gcggtttttc gaaggttaag tcagttgggg
     4741 aactgcttaa cctggtaact ggcttcagca gagcgcagat acc
//
