LOCUS       NZ_CP135267             4935 bp    DNA     circular CON 07-OCT-2023
DEFINITION  Enterobacter asburiae strain FAHZZU5722 plasmid unnamed5, complete
            sequence.
ACCESSION   NZ_CP135267
VERSION     NZ_CP135267.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37542530
            Assembly: GCF_032301015.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter asburiae
  ORGANISM  Enterobacter asburiae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4935)
  AUTHORS   Li,C.
  TITLE     Coexistence of blaNDM and mcr-10 in Enterobacter asburiae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4935)
  AUTHORS   Li,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2023) The first affiliated hospital of Zhengzhou
            University, Zhengzhou University, 100 Science Avenue, Zhengzhou,
            Henan 450001, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. v3.10.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/07/2023 07:06:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,003
            CDSs (total)                      :: 4,885
            Genes (coding)                    :: 4,745
            CDSs (with protein)               :: 4,745
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 35 of 140
            Pseudo Genes (incomplete)         :: 97 of 140
            Pseudo Genes (internal stop)      :: 25 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            Pseudo Genes (short protein)      :: 1 of 140
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP135267.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4935
                     /organism="Enterobacter asburiae"
                     /mol_type="genomic DNA"
                     /strain="FAHZZU5722"
                     /host="Homo sapiens"
                     /db_xref="taxon:61645"
                     /plasmid="unnamed5"
                     /geo_loc_name="China"
                     /collection_date="2022-03-04"
     gene            join(4484..4935,1..163)
                     /locus_tag="RQP58_RS24945"
                     /old_locus_tag="RQP58_24945"
     CDS             join(4484..4935,1..163)
                     /locus_tag="RQP58_RS24945"
                     /old_locus_tag="RQP58_24945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012822468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="WP_019725132.1"
                     /translation="MGKHKVFISYHHANDQYYKNALEKMNEEHEIFVNRSVSLGDIDED
                     EAPQKIREIIRDEYLRDTSVLILLVGTETKNRKHVDWELYSSMRDSTINKKSGILVVNL
                     PSTNTTYIRSTHGTNEKSEVHPTVTEWFSIDKRSTYEERFPYMPARIIDNFMKSGSYIS
                     VVNWNQIENNPETLRKLIDMTYNDREKATYDMSREMRMHNS"
                     /name="TIR domain-containing protein"
     gene            complement(200..907)
                     /locus_tag="RQP58_RS24950"
                     /old_locus_tag="RQP58_24950"
     CDS             complement(200..907)
                     /locus_tag="RQP58_RS24950"
                     /old_locus_tag="RQP58_24950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004198042.1"
                     /translation="MKTFVTLWTIVFLAFIAVMCTVDISNYIPSVYTVFSKNRPLVTGA
                     LSILLISVFIWLMVSLYLLKKYSVKVEKLSLGGVNILFNESATLYKKSVKNHLDSKRAI
                     FKLRKNVDAFDEVISSYYQTYQFIRDEMKILNPKKDREIYDFSNEMLMVLNRFLTNYQN
                     NYKRWYKYISDKDEVVDRVSGDVMRFHATSINKIQMQYYDYERILKGFKDVNRFFVNDV
                     KPKFGINTDKWNW"
                     /name="hypothetical protein"
     gene            complement(904..1809)
                     /locus_tag="RQP58_RS24955"
                     /old_locus_tag="RQP58_24955"
     CDS             complement(904..1809)
                     /locus_tag="RQP58_RS24955"
                     /old_locus_tag="RQP58_24955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_019725130.1"
                     /translation="MHNAALELFNERLPHKPYFSDDLHFGVRIAGKERAILAKYIQFNQ
                     PHAMFWLGFDVDRAGSSIDWSDRNAPAPTLTITNPENGHAHLLYALENSIRTAPDGKIK
                     PLRYAASVENALRKKLEADAGYSGLICKNPNHNHWKIAVWQPKLYTLDWLADFLDLNTG
                     NDKEIVADYGLGRNCTLFDKTRKWAYRAIRQGWPEYEQWLQACYERACAYNLQFARPLD
                     ENEVRGIAKSIAKWTSNKFSPEEFSKFVDITHSSEIQSKRGKKSGQSRRKGSLEEIKPW
                     VAMGISRRKYFYIKKNGEIR"
                     /name="replication initiation protein"
     gene            complement(2057..2332)
                     /locus_tag="RQP58_RS24960"
                     /old_locus_tag="RQP58_24960"
     CDS             complement(2057..2332)
                     /locus_tag="RQP58_RS24960"
                     /old_locus_tag="RQP58_24960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017412855.1"
                     /GO_process="GO:0008219 - cell death [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="WP_019725129.1"
                     /translation="MSKWNIKFTRNGADDLNYWRDTDKKKFERIRQLLLSIEADPLTGI
                     GKPERLRHHKDPALYSRRIDQSHRLVYSIQNGEIIIYAARYHYGDK"
                     /name="Txe/YoeB family addiction module toxin"
     gene            complement(2313..2558)
                     /locus_tag="RQP58_RS24965"
                     /old_locus_tag="RQP58_24965"
     CDS             complement(2313..2558)
                     /locus_tag="RQP58_RS24965"
                     /old_locus_tag="RQP58_24965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012848468.1"
                     /GO_component="GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0097351 - toxin sequestering activity [Evidence IEA]"
                     /GO_process="GO:0045927 - positive regulation of growth
                     [Evidence IEA]; GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="WP_025987695.1"
                     /translation="MHAINFTDARKHFAETMKRVTDDAEPVRVMRRDAPDIVMIDAGEY
                     EALIETVYLFSNPANAEHINESMKQAERGEFVEVEY"
                     /name="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
     gene            2795..2956
                     /locus_tag="RQP58_RS24970"
                     /old_locus_tag="RQP58_24970"
     CDS             2795..2956
                     /locus_tag="RQP58_RS24970"
                     /old_locus_tag="RQP58_24970"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_162870772.1"
                     /translation="MAKHWSGQECPKTGTYGQYHDTDNSYAGSEYDRHVSKGDKFPPSK
                     NNHHFQEK"
                     /name="hypothetical protein"
     gene            complement(3757..4158)
                     /locus_tag="RQP58_RS24975"
                     /old_locus_tag="RQP58_24975"
     CDS             complement(3757..4158)
                     /locus_tag="RQP58_RS24975"
                     /old_locus_tag="RQP58_24975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_045328244.1"
                     /translation="MTRVSISEAARLTGKSRTTLHRLIKTGELSTCSGERNKKMVDISE
                     LLRVFGPFDQPKNEHLTRQVPEQRDTAPADQSEHVIRQLKQQIEHLNILILSKDSHIDS
                     LKQAMLLLENHQAKLTEPPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
ORIGIN
        1 aatagacaac ttcatgaaga gcgggtctta tatatcagtg gtcaactgga atcaaataga
       61 aaacaaccca gaaacactca gaaaattaat agacatgact tataacgata gagaaaaagc
      121 aacgtatgat atgtcgagag aaatgagaat gcataattca tagtactatc caaggagtag
      181 ccttggctac tcctttaatt taccagttcc acttatctgt attgattcca aacttaggct
      241 tcacatcatt aacaaagaac cggttaacat ctttaaaacc ttttaaaata cgctcataat
      301 cataatactg catttgtatt ttatttatgc tggttgcatg aaatctcatg acatcaccgc
      361 taaccctatc aacaacctca tctttgtctg atatgtactt ataccaacgc ttgtagttgt
      421 tctggtagtt agttagaaat ctatttaaca ccatgagcat ctcattgctg aaatcataaa
      481 tctcacgatc ttttttaggg tttagtattt tcatttcatc acgaataaat tggtaagtct
      541 gataatatga tgaaataacc tcatcaaagg catcaacgtt ttttcttaat ttgaaaattg
      601 ctctttttga atctaggtga ttttttacac tctttttgta aagtgtagca ctctcattga
      661 aaagtatatt cactccacca aggctaagtt tctcaacctt aaccgaatac tttttaagta
      721 agtagaggga taccatcagc caaataaaaa cagatatcaa aagaatagaa agggcgccag
      781 ttacaagagg tctattctta ctaaaaactg tgtaaacaga cggaatgtaa ttagatatat
      841 caacagtaca catcacggca ataaatgcta aaaaaactat agtccaaaga gttacgaagg
      901 ttttcatctt atttcaccat ttttcttgat ataaaaatac ttccgcctgc ttattcccat
      961 agctacccaa ggttttattt cctctaggct tcctttcctc cttgactgcc ctgacttttt
     1021 ccctctcttt gattgaattt cagaggaatg agtaatatca acaaatttac tgaattcttc
     1081 aggtgaaaat ttatttgatg tccatttagc aatgctctta gctatccctc ttacctcatt
     1141 ttcatccaga ggccgtgcaa attgcaggtt gtatgcgcaa gcgcgttcgt agcaggcttg
     1201 cagccactgc tcatactcag gccagccctg acggatggcg cgatatgccc acttacgggt
     1261 tttgtcgaac aacgtgcagt tacgacctaa gccgtagtcg gcaacgattt ccttgtcatt
     1321 ccctgtgttc agatcgagaa agtcggccag ccagtccaga gtgtagagct tcggttgcca
     1381 cacggcgatt ttccagtggt tgtggttcgg atttttgcag atgagccccg aatatccagc
     1441 gtcagcctct aattttttac gtagcgcatt ctccacggag gcggcgtagc gtaacggttt
     1501 tattttgccg tccggcgcgg tgcgtatcga gttttccagc gcgtacagca ggtgcgcgtg
     1561 tccgttttcg gggttagtta tggtcagcgt cggtgcaggc gcgttgcggt cgctccagtc
     1621 gatggaggaa ccggcgcggt caacgtcaaa tcccagccag aacatggcgt gaggctgatt
     1681 gaactggata tatttcgcca gaatagcgcg ttccttaccg gcgatacgga caccaaagtg
     1741 cagatcgtcc gaaaagtacg gcttgtgtgg taagcgttcg ttgaaaagtt cgagtgcggc
     1801 gttatgcaat gtgccctgcg cttatggcgg gctgttgtct ggcacggttc atgtgctaat
     1861 atcctttcaa ggtttcgacc tagccctggt tatcatgcga tccgccaaga tatgacatga
     1921 tttccggggc tttgtttttt ctgaggctaa cgttacaccg actttgcctt tgtgtcgatc
     1981 tgacagcgac ttaccctaac atccgttgta cgccttgcat gaaaagctcg catcaagcgg
     2041 gctatatcga cttttgctat ttgtcaccat agtgataacg ggctgcgtag atgatgattt
     2101 ctccgttctg gattgaatag accagacggt ggctctgatc aatcctgcgg gagtagagtg
     2161 ccggatcttt gtggtgccgc agccgttcgg gttttcctat acctgtcagc ggatcagcct
     2221 cgatgctgag caacagttgc cggatacgct caaacttctt tttgtcagta tcacgccagt
     2281 agttaaggtc atcagcgcca ttcctagtga acttaatatt ccacttcgac aaattcgccg
     2341 cgctccgcct gtttcattga ctcattgata tgctctgcgt tcgccggatt gctgaacaga
     2401 tagaccgttt cgattagcgc ctcatactct cccgcatcga tcataacaat atcaggcgca
     2461 tcacggcgca tgacacgtac cggttcagca tcatcagtga cgcgcttcat cgtctcggca
     2521 aaatgtttac gggcatcagt aaaattgatt gcatgcatgt tgttaacccc catttgttgg
     2581 ggcttagcat agaacatgta cgcaaataag tacataaaga tatttcccta aaaacccaca
     2641 accaaatcag ctcaaaatgt gaactcaatc gaataaccac aaaaacaaca agtcaataca
     2701 ttgaagcata atttcaagca attacattta tgaagtgcgg cacaatttcg taccgctagg
     2761 agcttagctc ctaaaacgag caaaggaaaa gtaaatggca aaacattggt ctgggcaaga
     2821 atgtccaaaa acaggtactt acggtcaata tcatgatacc gacaactcat acgctggttc
     2881 tgaatatgat cgccatgtga gcaagggtga taagttccct ccgagcaaaa acaaccacca
     2941 cttccaagaa aaataaaata tcttcagaag tctaaactaa aagccctctg gggctttttt
     3001 tgtacctgcc attcacttga tttaccataa catccgttat cagcaccgcc tgagaggccc
     3061 tcagggcgtt tctgacggga aaccggtaaa acaaggcaac aaaccacccg aaaacccgcc
     3121 agaaacgcgc aggctctgtt tttacggcat gcatgagtat gcgcacaagt gcatagcgcg
     3181 gaaattgaca ccattcagcc aggggaagtc ctgtttccgg cgttcttccc atccgaaaac
     3241 ggtgaacggg gacgcaattt attgcgtggc cgtgaagcgt tgccggcgca gccggaaaac
     3301 cggttttcag cacgttcagg aatcggttcg tccacggccg gcaatcacgt tatggcaagt
     3361 cgccggacga ctcaccacac gattcaccgg cgactcttca ctagagtcgt acccagagtc
     3421 gccgcgtcta aaaaacaacc aaacggggag tcggatctag agtcgggtcg ttattaactc
     3481 attgttttta taggcctgag tcgttaccga ctctatgacg actcgccggg gactctatga
     3541 cgagtcgtca tagagtcgga tctatataaa taaaatttaa aaaacgaaat ttcagtaaag
     3601 taagctgctg atattaaata caaatctgca aaattttaat tttgcgtggg gtgtgggtat
     3661 ttttcgtggt gaggagcgtt cagagatctg acgattttcg ggaaggttca ggaactcccc
     3721 ggaatggccg gagaggaaaa ctgaagcatg acggggtcag gattttttcc agaatttcca
     3781 ccacggcgcc gggggttcag ttaattttgc ctggtgattt tcaagaagga gcatggcctg
     3841 tttcaggctg tcaatgtgcg aatccttgga taaaatcaat atgttaaggt gttcaatctg
     3901 ctgtttcagt tgacgaatga catgttcact ctggtcagca ggcgctgtat cacgctgttc
     3961 aggtacttgt cgagtaaggt gttcattctt cggttggtca aacggtccga aaacacgtaa
     4021 aagctctgat atatcgacca tttttttatt tcgttcaccg ctacaagtgg acagttcacc
     4081 tgttttgatt aacctgtgca gcgttgtccg gctcttaccc gtcagccgtg cagcctcact
     4141 aatagacact ctggtcatca gttatcatcc tttaatacat tcagtctcaa ggaaacaggg
     4201 gccagcttac gacatactgg tccagaagtg atgtgttttt cggaagggcg atgaaacaca
     4261 ggagtgaccg tgcaaaatgc aggcaacaaa tgactttccg cttcctcgcg cattcgctca
     4321 ctacgttcaa cgagcccgcc ctaaaccagt catggtgaga ccttgctgaa gggaccctat
     4381 gtgtgctggc tgagaaaaaa agagctctct aaaagagagc tgttatctga tataggttca
     4441 agtgcaaaat agagtgcaca caaacaaaaa aaggacaata aaaatgggaa agcataaggt
     4501 attcattagc taccaccatg cgaatgatca gtactacaaa aacgcacttg aaaaaatgaa
     4561 tgaggaacat gaaatattcg tgaatcgctc agtatcatta ggtgatatag atgaagatga
     4621 agccccacaa aaaataagag agataataag agatgaatat cttagagata catcagttct
     4681 tatccttctt gttggaactg aaacaaaaaa cagaaaacac gtagattggg aactttactc
     4741 tagcatgaga gatagtacca ttaacaaaaa atcaggaatt ttagtcgtca atctcccgtc
     4801 aacaaatact acgtacattc gttcaacaca tggaactaat gaaaaatcag aagttcatcc
     4861 taccgtaact gaatggtttt ctattgataa gcgctcaact tatgaggaaa ggttcccata
     4921 tatgccagca agaat
//
