LOCUS       NZ_CP135266             5286 bp    DNA     circular CON 07-OCT-2023
DEFINITION  Enterobacter asburiae strain FAHZZU5722 plasmid unnamed4, complete
            sequence.
ACCESSION   NZ_CP135266
VERSION     NZ_CP135266.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37542530
            Assembly: GCF_032301015.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter asburiae
  ORGANISM  Enterobacter asburiae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 5286)
  AUTHORS   Li,C.
  TITLE     Coexistence of blaNDM and mcr-10 in Enterobacter asburiae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5286)
  AUTHORS   Li,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2023) The first affiliated hospital of Zhengzhou
            University, Zhengzhou University, 100 Science Avenue, Zhengzhou,
            Henan 450001, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. v3.10.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/07/2023 07:06:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,003
            CDSs (total)                      :: 4,885
            Genes (coding)                    :: 4,745
            CDSs (with protein)               :: 4,745
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 35 of 140
            Pseudo Genes (incomplete)         :: 97 of 140
            Pseudo Genes (internal stop)      :: 25 of 140
            Pseudo Genes (multiple problems)  :: 15 of 140
            Pseudo Genes (short protein)      :: 1 of 140
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP135266.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5286
                     /organism="Enterobacter asburiae"
                     /mol_type="genomic DNA"
                     /strain="FAHZZU5722"
                     /host="Homo sapiens"
                     /db_xref="taxon:61645"
                     /plasmid="unnamed4"
                     /geo_loc_name="China"
                     /collection_date="2022-03-04"
     gene            297..620
                     /locus_tag="RQP58_RS24905"
                     /old_locus_tag="RQP58_24905"
     CDS             297..620
                     /locus_tag="RQP58_RS24905"
                     /old_locus_tag="RQP58_24905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_163063187.1"
                     /translation="MLTMWVTEGEHRRLLERCDGRPLAAWMRQTCLDEKPARTGKLPSL
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            829..>1263
                     /locus_tag="RQP58_RS24910"
                     /old_locus_tag="RQP58_24910"
                     /pseudo=""
     CDS             829..>1263
                     /locus_tag="RQP58_RS24910"
                     /old_locus_tag="RQP58_24910"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1298..1789
                     /locus_tag="RQP58_RS24915"
                     /old_locus_tag="RQP58_24915"
     CDS             1298..1789
                     /locus_tag="RQP58_RS24915"
                     /old_locus_tag="RQP58_24915"
                     /inference="COORDINATES: protein motif:HMM:NF016710.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_213757678.1"
                     /translation="MSSLLTLAKDLEQQSKAQKQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAILAYEQSMSEAMEKNRRSVLRTAGRTWLTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            1796..2011
                     /locus_tag="RQP58_RS24920"
                     /old_locus_tag="RQP58_24920"
     CDS             1796..2011
                     /locus_tag="RQP58_RS24920"
                     /old_locus_tag="RQP58_24920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137422.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            2657..3354
                     /locus_tag="RQP58_RS24925"
                     /old_locus_tag="RQP58_24925"
     CDS             join(2657..2906,2906..3354)
                     /locus_tag="RQP58_RS24925"
                     /old_locus_tag="RQP58_24925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558267.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="WP_227674736.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRASARIMGVGLNTVLRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLATLERLMSLLSPFDVVIW
                     MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1 family transposase"
     gene            complement(<3369..3545)
                     /locus_tag="RQP58_RS24930"
                     /old_locus_tag="RQP58_24930"
                     /pseudo=""
     CDS             complement(<3369..3545)
                     /locus_tag="RQP58_RS24930"
                     /old_locus_tag="RQP58_24930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /name="IS1 family transposase"
     gene            complement(3697..4359)
                     /locus_tag="RQP58_RS24935"
                     /old_locus_tag="RQP58_24935"
     CDS             complement(3697..4359)
                     /locus_tag="RQP58_RS24935"
                     /old_locus_tag="RQP58_24935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000430080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="WP_072661022.1"
                     /translation="MEKNSSDIDVIELFLFLKNKIMSIILFVIFSLILSSIFLLINKNK
                     ISVKYELNMITNSPGMIINCGDDFYCKANMIQSIIKNKSNSITSNIDERGKKINLSWAG
                     DDRYKPSVTAEVNAIHKAINDWYIQDYKTYKSIVNNQDSNYINGTETYAKIALLTKTNT
                     SGERNFISINNEIVNKKYKPALIMTLTLIMAIIFSSSYHIIKRSVLDYKNKSNRSFY"
                     /name="GNVR domain-containing protein"
     gene            complement(4786..4932)
                     /locus_tag="RQP58_RS24940"
                     /old_locus_tag="RQP58_24940"
     CDS             complement(4786..4932)
                     /locus_tag="RQP58_RS24940"
                     /old_locus_tag="RQP58_24940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063922569.1"
                     /translation="MHEPPVQSDRCASSGNFQLDSNPERHDKAPLAAATGKRVYKRLLI
                     QSS"
                     /name="hypothetical protein"
     Typing          9..182
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.0"
                     /coverage="100.0"
                     /evalue="1.21e-74"
     Typing          complement(4764..5286)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.366"
                     /coverage="86.0"
                     /evalue="0.0"
     Typing          298..614
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.453"
                     /coverage="99.0"
                     /evalue="1.45e-59"
     Typing          611..2103
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.4"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cgagtcagtg agcgaggaag cggtatatat tctgtatcac atattctgct gacgtaccgg
       61 tgccgccttt tttctcctgc cacatgaagc acttcactga caccctcatc cgtgtcaaca
      121 tattaagcca gtatacactc cgctagcgct acgtgactgg ttcagggctg cgccccgaaa
      181 cccgccaaaa gccactgacg cgcctgcggc ttgtccaacc ccgcgccgac cgggaaaggc
      241 tttcccgccc gtcccggcgt tatcaggagg ccggtttgtc agagaaacgg aacaagatgc
      301 tcaccatgtg ggtgacggag ggcgagcacc ggcggctgct tgagcgctgc gacggcaggc
      361 cgctggcggc gtggatgcgg cagacctgcc tggacgaaaa gcctgcgcgc accggaaagc
      421 tcccctcact ctcgccggcg ctgctgcgcc agcttgccgg tatgggcaat aacctgaacc
      481 agatagcccg ccgtgttaac gccggcggcg gcaccggcca tgacagggta cagattgtgg
      541 cggcgctgat ggccatcgat gccgggcttg agcggctgcg gcacgccgtt cttgagaaag
      601 gaacggacga tgatcgttaa atttcatcca cgcggtcgcg gcggtggcgc cgggccggtg
      661 gactatctgc tgggtaaaga ccggcagcgt gaaggcgcca gcgtcctgca ggggaagccg
      721 gaagaagtcc gggagctgat tgacgcgtcg ccttacgtga agaaatacac ctccggcgta
      781 ctgtcgtttg cggaggccga tctgcctccc ggccagcggg aaaaactgat ggccagcttc
      841 gagcgggttc tgatgcccgg actcgataaa gatcagtaca gcattctgtg ggtggagcac
      901 gcggacaagg ggcgtctgga gctgaacttt ctgatcccca ataccgaact gctgaccggc
      961 aggcggctcc agccgtacta cgaccgcgcc gaccggccgc gcatcgatgc ctggcagacc
     1021 atcgtgaacg gcagactggg gctgcatgac ccgaacgcgc cggaaaaccg ccgggcgctg
     1081 gtcacgccgt ccggactgcc gaaaacgaag caggaggcca ctgaagccat cacgcgcgga
     1141 ctgctgaccc ttgcttcgtc cggggagctg aaaaaccgtc aggacgtcac tgaggcgctg
     1201 gaacggtcag gttttgaggt ggtgcgcacc acgaaaagca gtatcagcat tgccgacccg
     1261 gacggggggc gaaacatccg actgaaggga gccatctatg agcagtcttt taacgctggc
     1321 aaaggactta gagcagcaat cgaaagcgca gaagcagagt accggcgaga tgctgaaagc
     1381 cgcattcagc gagcacgagc agtctgtcag agcggaactg agcgcaagcg cgaggagaat
     1441 cagcgacgcc atcctcgcct acgagcagag tatgagcgag gcgatggaga aaaaccggcg
     1501 gagcgtactg cggactgccg ggcggacatg gctcaccatc ctgatggtgt cagcgctgct
     1561 gatcggcacg agcgggagca ttctgtggtg gcaggggcag cagataaccg acaattacac
     1621 gcatcttcgt cagcaggaag ataccctggc gaaaatgacc gcccggacgt ggggagtgcg
     1681 ctatcaggag agcagcgacg gcaggaggtt tcttatcctg ccgccgggga tgcaaaccga
     1741 agccatcccc tacgacggga cgacatggat ccgcctgaaa caggagtagc ggaaaatgac
     1801 agagctggaa aagcagttgc tgagcgcatt agagcagcta cagcaggact actcgcaaag
     1861 gctggacgaa tgggagagcg ccttcgcgga atggcggagc atgtgtgggc ttatgcaacg
     1921 ggagaacgcg gtgctgaacg agcgcgtgtc ggacttgagc acgcaggtgc tgagtttaag
     1981 cgagcagctg cgccgcttgt ccgggaactg aatgccatcg agcttcggca gagggagaat
     2041 gagtatcaga aaacactgga gctggagcgc cagccgcaga aaacctaccg cgggccgaca
     2101 ctttaaacca tccaggcagc tgacaacccg cacgaagagg ggctcttatt tctcccgcta
     2161 aataggttcg tgatcaaaaa ggtcacgact tattcaggag cgggaaatga agctgttaaa
     2221 tcttattgaa tcattgctta actgcctgta tctggcggcg gagctgtggg actggctgga
     2281 gaagcatttg tcggttttta aggcattggc gcggatttta gtgtgacttt aacatccatg
     2341 catctggctg catgactatg caccagtgta atattcagcg gctgcgtttc tggtgttttt
     2401 tcgttatgtc tgtcgccact ttctcgcgtc tggcaccgga aacgcagcca accccgtttc
     2461 agcggtgcgc ccaacggatg ttatggtaaa ttaggcgcat gataactcat tccgtatata
     2521 ttattttctt tagtaacgta tggacaggat attggtaaat ggattcagta aaaaaaacgg
     2581 gattgatgaa gctgaaatgc aggtaatgct gccaacttac tgatttagtg tatgatggtg
     2641 tttttgaggt gctccagtgg cttctgtttc tatcagctgt ccctcctgtt cagctactga
     2701 cggggtggtg cgtaacggca aaagcaccgc cggacatcag cgctatctct gctctcactg
     2761 ccgtaaaaca tggcaactac agttcactta caccgcctct cagcccggta cgcaccagaa
     2821 aatcattgat atggccatga atggcgtcgg atgtcgcgcc agtgcacgca ttatgggcgt
     2881 tggcctcaac acggttttac gtcacttaaa aaactcaggc cgcagtcggt aacctcgcgc
     2941 atacagccgg gcagtgatgt gattgtctgc gctgaaatgg acgaacagtg gggctacgtc
     3001 ggtgctaaat cacgtcagcg ctggctgttt tacgcgtatg acaggatacg gaggacggtt
     3061 gtggcgcacg tcttcggtga acgcactctg gccacactgg agcgtcttat gagcctgctg
     3121 tcaccctttg acgtggtgat atggatgacg gatggctggc cgctgtatga atcccgcctg
     3181 aagggaaagc tgcacgtaat cagcaagcga tatacgcagc gaattgagcg gcataacctg
     3241 aatctgaggc agcacctggc acggctggga cggaagtcgc tgtcgttctc aaaatcggtg
     3301 gagctgcatg acaaagtcat cgggcattat ctgaacataa aacactatca ataagttgga
     3361 gtcattaccg ccatttcagt gatcagctct ttcatacccg gcttacgtgc ttgataagta
     3421 taagtgagct gaaatacacg gtggcagtca cggcagcgaa atctgtcacg gcctttaggg
     3481 ttctgaccat ggcggtaaac cagagctgac tgacaacggg gacaatgaat attaacgctg
     3541 gctatgagat aacctcaaaa gcccgtatta tacatcagat tcaactaatt agaggcatca
     3601 cccagtgaga tttgtcggga ggcccgctat tagcgggctt ttctgcatca ggcgtgtaac
     3661 ggatgttgct agaatacggt tattaatcgt tacaggctaa taaaaagacc tatttgactt
     3721 atttttatag tcaagtacag atctttttat tatatggtac gaggaggaaa agataatagc
     3781 cataattaac gttagagtca taattaaagc tggtttatac tttttattta ctatttcatt
     3841 attgattgaa ataaagtttc tttctccgga agtgtttgtt ttagttaata atgcaatttt
     3901 tgcatatgtt tcagtgccat ttatatagtt gctatcctgg ttgttaacta tgcttttata
     3961 agttttataa tcttggatgt accagtcatt aatagcttta tggatagcgt ttacttcagc
     4021 ggtaaccgag ggtttgtaac ggtcatcacc agcccatgac aggtttattt tttttcctct
     4081 ttcatctata tttgatgtta tagaattgct tttgtttttt attattgatt gtatcatgtt
     4141 cgctttgcaa taaaagtcat ccccacagtt aataatcata cctggcgaat tagtgatcat
     4201 atttaattcg tattttacac ttattttgtt tttgtttatt aacaaaaaaa tagatgacaa
     4261 aatcaatgag aaaataacaa acaaaataat actcattatt ttgtttttga gaaatagaaa
     4321 taactcgata acatcaatat cagatgaatt tttttccatt tttatatacc aataatattt
     4381 attaaagtat gggttaaata aataaataaa attaaaaagg ctgtgatgaa tatttttgcg
     4441 gatttagtgt atttcatttt aaaagccaat cgaataaaaa aacattttac ctactattta
     4501 ggtgtgaaaa aactcctaaa atcatttttt ttgtttcttt tttgttcggt catgtctatg
     4561 gagggagttg ttgttagcct gtatcctttc aacatatttt aagatcttct tgagatcctt
     4621 gcgttacagc cgtaatctct tgctctgaaa acgaaaaaac cacctgggga ggtggttttt
     4681 cgaaggttca gtaagttggg gaacttctga accgtggtaa cagggtgtac aagaccgctg
     4741 ccaccaaatt cgtccttcca gtttagccgt agtttggctc tgacttcaag aactctgtat
     4801 cagtaatctc ttgtacaccc tcttaccagt ggctgctgcc agtggcgctt tgtcgtgcct
     4861 ttccgggttg gaatcaagtt gaaagttacc ggaggaggcg cagcggtcgg actgaacggg
     4921 gggttcgtgc atacagtcca gcttggagcg aactgccttc ccggaactga gtgtcaggcg
     4981 tggaatgaga taaacgcggc cataacagcg gagtgacacc ggtaaaccga aaggcaggaa
     5041 caggagagcg cacgagggag ccaccagggg gaaacgcctg gtatctttat agtcctgtcg
     5101 ggtttcgcca ccactgattt gagcgtcaga tttcgtgatg cttgtcaggg gggcggagcc
     5161 tatggaaaaa cggctttgcc gcggccttat cgcttgcccg ttaagtttct tcctggtatc
     5221 caccaggaaa tttccgcccc attcgtaagc ccgcaccgct cgccgcagcc gaacgaccga
     5281 gcgcag
//
