LOCUS       NZ_CP135258             4099 bp    DNA     circular CON 23-OCT-2023
DEFINITION  Enterobacter chuandaensis strain FAHZZU5885 plasmid pFAHZZU5885-6,
            complete sequence.
ACCESSION   NZ_CP135258
VERSION     NZ_CP135258.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37542559
KEYWORDS    RefSeq.
SOURCE      Enterobacter chuandaensis
  ORGANISM  Enterobacter chuandaensis
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4099)
  AUTHORS   Chen,R.
  TITLE     Coexistence of blaNDM-1 and blaKPC-2 in Enterobacter chuandaensis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4099)
  AUTHORS   Chen,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2023) the first affiliated hospital of Zhengzhou
            University, Zhengzhou University, 100 Science Avenue, Zhengzhou,
            Henan 450001, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. v3.10.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/23/2023 12:24:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4
            CDSs (total)                      :: 4
            Genes (coding)                    :: 3
            CDSs (with protein)               :: 3
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP135258.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4099
                     /organism="Enterobacter chuandaensis"
                     /mol_type="genomic DNA"
                     /strain="FAHZZU5885"
                     /host="Homo sapiens"
                     /db_xref="taxon:2497875"
                     /plasmid="pFAHZZU5885-6"
                     /geo_loc_name="China"
                     /collection_date="2022-03-31"
     gene            650..1036
                     /gene="stbA"
                     /locus_tag="RQP59_RS00005"
                     /old_locus_tag="RQP59_25375"
     CDS             650..1036
                     /gene="stbA"
                     /locus_tag="RQP59_RS00005"
                     /old_locus_tag="RQP59_25375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006796897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein StbA"
                     /protein_id="WP_001039464.1"
                     /translation="MNKPADLKPRNLSAAVRLRLNEIENWLDRGLTRHEIAEILDSEYS
                     FSVTAKGLEMALYRTRQNRKNVLHNTHDKSSAKGAAESVLHNTQPSEPEVQESKKAESP
                     GIIDKEFFKKIGENFDPKKFNKKF"
                     /name="stbA"
     gene            complement(1176..2144)
                     /locus_tag="RQP59_RS00010"
                     /old_locus_tag="RQP59_25380"
     CDS             complement(1176..2144)
                     /locus_tag="RQP59_RS00010"
                     /old_locus_tag="RQP59_25380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611102.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="WP_030005799.1"
                     /translation="MKDQITHLPDNADHSVAKQKFKITNWPTYNKALINRGSITFWLDD
                     EAIQAWYESATPSSRGRPQRYSDLAITTVLVIKRVFRLTLRAAQGFIDSIFTLMNVPLR
                     CPDYTSVSKRAKSVNVSFKTFTRGEIAHLVIDSTGLKVFGEGEWKVKKHGQERRRIWRK
                     LHLAVDSNTHEIICANLSLNNVTDSEAFPGLIRQTHRKIRAASADGAYDTQLCHDELRR
                     KKISALIPPRKGAGYWPGEYADRNRAVANQRLTGSNARWKWTTDYNRRSIAETAMYRVK
                     QLFGGSLTLRDYDGQVAEAMALVRALNKMTKAGMPESVRIA"
                     /name="IS5 family transposase"
     gene            complement(2992..>3300)
                     /locus_tag="RQP59_RS00015"
                     /old_locus_tag="RQP59_25385"
                     /pseudo=""
     CDS             complement(2992..>3300)
                     /locus_tag="RQP59_RS00015"
                     /old_locus_tag="RQP59_25385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561120.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            complement(3310..4007)
                     /locus_tag="RQP59_RS00020"
                     /old_locus_tag="RQP59_25390"
     CDS             complement(join(3310..3758,3758..4007))
                     /locus_tag="RQP59_RS00020"
                     /old_locus_tag="RQP59_25390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558268.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1X3 family transposase"
                     /protein_id="WP_223229472.1"
                     /translation="MASISISCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTILRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEQWGYVGAKSRQRWLFYAYGRMCRTVVARVFGERTLATLERLPGLLSAFEVVVW
                     MTDGWPLYESRLKGELHVISKRYTQRIERHNQNLRQHLARLGRKSLSFSKSVALHDKVI
                     GHYLNIKHYQ"
                     /name="IS1-like element IS1X3 family transposase"
     Typing          complement(167..424)
                     /name="MOBF"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="87.072"
                     /coverage="100.0"
                     /evalue="4.07e-76"
ORIGIN
        1 tattttgaac ttttgctttg ccacggaacg gtctgcgttg tcgggaagat gcgtgatctg
       61 atccttcaac tcagcaaaag ttcgatttat tcaacaaagc ccattcagtc ctcaatttgt
      121 gcatttttta aacgaaaaaa gggtgtctaa tacggtgaaa tcatagtatt accaaagtaa
      181 taaataaaaa gttatttaaa tcagtaattt agatttgatt tgtaatacct aggtaatacc
      241 gcagcatggc taaattttgt tttttttctt ttaaatcagt gcgatagcgt gatttatcac
      301 gctgcgttag gtgtatagca ggttaaggga taaaaaatca tctttttttg gtagggcgat
      361 ctccgtaggt taaggggcat ttggctaaaa agcgtcctat tctttgatgg tcatgcttgc
      421 atgaccatct gagcaaccaa aaactacaga taaactacag ataaactaca aaaaacgttt
      481 tgccttagtg ttgtaagact acaaatagac tacaataaaa ctacaaataa actacaaaaa
      541 acgtgacaga ctacaaatag actacaataa aactacagat aaactacaaa actcgattga
      601 ccccttctta cgagtgttgt agagtcattt tcatacaacg gagggggtta tgaataaacc
      661 agcagatctg aaaccccgca acttatcggc tgctgtcaga ttgcgcctaa atgaaatcga
      721 gaactggctg gacagagggc taacgcggca tgaaattgct gaaatcctcg acagcgaata
      781 cagcttttcg gtaacagcca aagggcttga gatggcactg tatagaacgc ggcaaaaccg
      841 aaaaaatgta ttgcacaata cgcatgataa gagtagcgcg aagggtgcag cggaaagtgt
      901 attgcacaat acacaaccgt ctgagcctga agtgcaggaa agtaaaaaag cagagagtcc
      961 cggcattatt gataaagaat tcttcaagaa aatcggtgag aatttcgacc ctaagaagtt
     1021 caacaaaaaa ttctgaggtg atttatgaaa gtagcggtaa ttaattacag tggcagtgtt
     1081 cgtaaaacct taatttcatc ttacctgtta gccccgcgcg gctttgttga ataaatcaga
     1141 tttcgggtaa gtctcccccg tagcgggttg tgttttcagg caatacgcac gctttcaggc
     1201 atacctgctt tcgtcatttt gttcagcgct cgtaccaggg ccatagcctc tgcaacctga
     1261 ccatcgtagt cacgcagtgt cagtgaacct ccgaacagct gttttacccg gtacatcgcc
     1321 gtttccgcta tcgagcgacg gttgtaatct gttgtccatt tccaccgcgc attactcccg
     1381 gtcagccgct gattcgcaac agcacggtta cggtctgcat attcaccggg ccagtaaccc
     1441 gcgccttttc ggggcgggat aagcgcgctg attttcttac gccgcagttc atcgtgacag
     1501 agttgggtgt cgtaagcgcc gtctgccgat gctgccctga tttttctgtg agtctgccgg
     1561 ataagacccg ggaaggcttc tgagtccgtc acattgttca gcgacaggtt tgcacagatg
     1621 atttcatgtg tgttgctgtc aactgccaga tgcaactttc gccatatacg acggcgttcc
     1681 tggccatgct ttttgacttt ccattcgcct tcaccaaaga ccttcagccc ggtggaatca
     1741 atcaccagat gcgcgatttc accccgggtg aacgttttga aactgacatt aaccgacttt
     1801 gcgcgcttgc tgacactggt gtaatccggg cagcgcaacg gaacattcat cagtgtaaaa
     1861 atggaatcaa taaaaccctg tgcagcccgc agggtcaacc tgaacacgcg tttaatgacc
     1921 agaacggtgg tgatggcgag atcagaatag cgctgaggtc ttccccgtga tgaaggcgtt
     1981 gccgactcat accaggcctg aatagcttca tcatccagcc agaaagttat ggagccacgg
     2041 ttgatgaggg ctttattgta ggtgggccag ttggttattt tgaacttttg ctttgccacg
     2101 gaatggtctg cgttgtcggg aagatgcgtg atctgatcct tcaactcagc aaaagttcga
     2161 tttattcaac aaagccgtct gtgttgtaaa atggcgccga aagcccggcg tagccggttg
     2221 ccgggctata aattctgcca ccctaccccc tgccctgatt agccttaact cgcgaacagc
     2281 aatgaagaag tcaaagtaat cccttttaac cccggtgagc gggctttacg gcgtttacga
     2341 cgtaaagggc gtgatttccg gggcctaagc ggcggcagct tgctgctgac gcttaggggg
     2401 atgttcatca actatttcag cgacaccagg gaacttaaca cggtagctgc aaagccctaa
     2461 tcccgctcac cgccacgcag ctgccgtacg tcccccacgg gggtgcgcag tgctgcgcgc
     2521 gggaacggcg gcccgtctgc gggacgcggc gccgtactgc gcgttagcgg ccgcacgcgg
     2581 ccggttacgg gggacactcc gccatacgac cagcgacccg ctgagctgca caatccacag
     2641 ataacacaag ctacatacat gtcgatatgg gaatcggccc accttgccgg gcgccggtgc
     2701 cgtgtggaaa tccgcccacc ttgccgcgcg ccggtgccgt gtgggaatcc gcccaccttg
     2761 ccgcgcgccg gtgccgtgtg ggaatccgcc caccttgccg ggcgccggtg ctgtgtggaa
     2821 atccgcccac cttaccgggc gccggtgctg tgtggaaatc cgcccatctt gccgggcgcc
     2881 ggtgccgtgt gggaatccgc ccaccttacc gggcgccggt gctgtgtgga aatccgccca
     2941 tcttcatgac aaaaatttgt caaatgacag gctcatgtgt gctgatttat gtcagacggg
     3001 attttgctta tcaattattg agaacagaaa ttttcctgaa tcatcaatac tatacttagc
     3061 aagtaaaggt gttttctcat ttatctgctt gagtgcagga tcaaggaaag atcgtttcaa
     3121 ctctgccaag ttcttctgct gggattccgg taagacgaaa cggtcattta accaagcatg
     3181 agtagtaacc cataagcctg aagatttgaa ttgagcaaga ctttcataaa ggcggatcgt
     3241 ccggggattt cgaagactgc cacaatcgcg gagcgagtag gtggtgaact ggttggtaat
     3301 gactccaact tactgatagt gttttatgtt cagataatgc ccgatgactt tgtcatgcag
     3361 cgccaccgat tttgagaacg acagtgactt cctgcccagc cttgccagat gctgcctcag
     3421 attctggtta tgccgctcaa ttcgctgcgt atatcgcttg ctgataacgt gcagttctcc
     3481 cttcaggcgg gattcataaa gcggccagcc atccgtcatc cataccacga cctcaaaggc
     3541 cgacagcagg cccggaagac gctccagcgt ggccagagta cgctcaccaa agacgcgtgc
     3601 caccaccgtc ctgcacatcc tgccataggc gtaaaataac cagcgctgac gtgatttagc
     3661 cccgacgtag ccccactgtt cgtccatttc cgcgcagaca atgacgtcac tgcccggttg
     3721 tatgcgtgag gttaccgact gcggtctgag ttttttaagt gacgtaaaat cgtgttgagg
     3781 ccaacgccca taatgcgggc ggttgcccgg catccaacgc cattcatggc catatcaatg
     3841 attttctggt gcgtaccggg ctgagaggcg gtgtaagtga actgtagctg ccatgtttta
     3901 cggcagtgag agcagagata gcgctgatgt ccggcagtac ttttaccgtt acgcaccacg
     3961 ccttcagtag ctgagcagga gggacaactg atggagatgg aagccactgg agcacctcaa
     4021 agacaccatc gtacactaaa tcagtaagtt ggcaccatta tccggcaggc gatctggatt
     4081 atcgtcacgc tgttaaata
//
