LOCUS       NZ_CP135252             4072 bp    DNA     circular CON 07-OCT-2023
DEFINITION  Escherichia coli strain OHT76-1 plasmid pOHT76-1_8, complete
            sequence.
ACCESSION   NZ_CP135252
VERSION     NZ_CP135252.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37300944
            Assembly: GCF_032300135.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   Wittum,T.
  TITLE     Salmonella and antimicrobial resistant bacteria recovered from
            retail pet treats in the US
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4072)
  AUTHORS   Li,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-SEP-2023) CVM/OAS, FDA-CVM, 8401 Muirkirk Rd, MOD2,
            Laurel, MD 20708, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-JUL-2021
            Assembly Method                   :: HGAP v. 4
            Assembly Name                     :: Microbial assembly
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 228x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/07/2023 07:18:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,262
            CDSs (total)                      :: 5,137
            Genes (coding)                    :: 4,891
            CDSs (with protein)               :: 4,891
            Genes (RNA)                       :: 125
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 93
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 95 of 246
            Pseudo Genes (incomplete)         :: 150 of 246
            Pseudo Genes (internal stop)      :: 47 of 246
            Pseudo Genes (multiple problems)  :: 42 of 246
            Pseudo Genes (short protein)      :: 2 of 246
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP135252.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="OHT76-1"
                     /isolation_source="Pig ear pet treat"
                     /host="Food"
                     /db_xref="taxon:562"
                     /plasmid="pOHT76-1_8"
                     /geo_loc_name="USA:OH"
                     /collection_date="2021-06-14"
                     /collected_by="The Ohio State University"
     gene            join(4050..4072,1..2137)
                     /gene="mobQ"
                     /locus_tag="RRJ52_RS26300"
                     /old_locus_tag="RRJ52_26300"
     CDS             join(4050..4072,1..2137)
                     /gene="mobQ"
                     /locus_tag="RRJ52_RS26300"
                     /old_locus_tag="RRJ52_26300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_001029353.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAECRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLLPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHTTERRHDRGWSQ"
                     /name="mobQ"
     gene            complement(2393..3085)
                     /locus_tag="RRJ52_RS26305"
                     /old_locus_tag="RRJ52_26305"
     CDS             complement(2393..3085)
                     /locus_tag="RRJ52_RS26305"
                     /old_locus_tag="RRJ52_26305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281403.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCISRLKSGADAELQKT
                     MTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKNDEKREEFRWIQYRAQYAKG
                     EAKARITFSDAVMPYLTQLKGQFTRVVIKNISGLSSSYSIRIYEFLQQFRSTGERIIAL
                     NDFRSMLGIENKYKQFRDLNKILIKPCVDELNKKSDLAVTVETIKKGRTVVALHFRFKE
                     DKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(3617..3871)
                     /gene="mobC"
                     /locus_tag="RRJ52_RS26310"
                     /old_locus_tag="RRJ52_26310"
     CDS             complement(3617..3871)
                     /gene="mobC"
                     /locus_tag="RRJ52_RS26310"
                     /old_locus_tag="RRJ52_26310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091747.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobC"
     Typing          2..336
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.917"
                     /coverage="93.0"
                     /evalue="8.65e-164"
     Typing          complement(3873..4049)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.438"
                     /coverage="100.0"
                     /evalue="2.610000000000001e-86"
     Typing          complement(2394..3085)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.965"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3..2134
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.578"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 gaaaattatc tccagaagca gcggctacag cgccgtagct tccgctgcct atcgttccgg
       61 ttcactgatg cttgatgagc gtaccggact gactcacgac tacacccgca aaagcggtgt
      121 ggctgaagct gtgattctga cccccgcgac agcccctgcc tggtgtacga accgtgccga
      181 actctggaat gccgttgaga aggccgaatg ccggaagaac tcacaacttg cgcgggaaat
      241 tgaactcgct atcccccgtg aactgccgca ggatgccgca cgggaaaccg ttctcgcctt
      301 tgtcagagaa aactttgtca gtcagggcat gattgctgat gtggcgttcc atcacatgga
      361 taaaacaaat ccccatgcgc acatcatgct gaccacgagg gctgtcggac ctgctgggtt
      421 cggcggaaag gtcagggact ggaacgaccg gacacatgcc gagacgtggc gtgcatcatg
      481 ggctgaccat gcaaacagag cactggcgaa cgccggttat caggaagaga tagaccatcg
      541 ttcatacgag cgacaggggc ttgagaaaac gcctggtatt cacctcggaa agtctgcctg
      601 tgcgatggaa acacgcggta tcgaaacaga acgcggcgaa cagaatcgtc tgattaacag
      661 gcttaacctg gaaatacaga tttcccgcac tgaactcagg aacagcgggc tgacagagcc
      721 tgcccgccgt gtggctgacc ttctgaatat cgtgcttcct gacaacgcaa cttcgtacac
      781 actacgtgac atcatcgaag cgttgccgaa ggacagtaat gcggcatgga aactcaccag
      841 taatgccatg tcgatgatgg cagatatgca ggcgacgcgc agacgctggg aagagctgaa
      901 caccgaacga aaggaagcga tgagccatgc tgccagcctg aaaaaatcat cgccgttttc
      961 gtccggtttt tcccgtattc ccctgatgca atgggcggca ccggaatacc gtagcgaaca
     1021 gggaaaaatc cagtcgctga gccagcaaat ggaaaaattg cgacagcatt accggaaggt
     1081 ggagaaacag gacattcccg cgagtcagac ggcatttgaa aaacagtggc agcagtgggg
     1141 ggcatccggc cttgcagctc tcagagtcca gttgcttaaa cgtgaagagg aattgcgccg
     1201 gaaggagcag gaggatacag aacgtagtcg cgcggagcga cgagcggaac agctcaggaa
     1261 aaatgacaat gccgttatgg attcaggggt actgagcgca gtcgttacgg ggtatggcga
     1321 ggcccccatg cctggtaacg gggaaatgac ctgttacctt ctgcttcaca accggaacgg
     1381 agaatacacg ctctggggga acgagctgga gaaataccgc accagaatct ttgagcgtgt
     1441 tgatttaatg cgtgacagga gtggttatat ctgtgaccga agcgaaatcg gtcaacatcc
     1501 accactgcaa cgtgtttatt cctctgcgac ctttgagcaa cttctgacgc aggtttgcca
     1561 gacatggccg caatataccc gcaatctccg ccagccgaag acatggccgg agtctttctg
     1621 tcttggagaa gaccgccagc cagccatgcc atcactggca gcaaggaagg tggattttac
     1681 gcagggacgt ctgctaccga cgctgatgcc ggtaatgagt tctgtcgacc gggaaacccg
     1741 ccagcttcag cttttgctgg tcatgggcgt ggatgactct ctgggcggtg ttgtccggct
     1801 taatggtacg ctgtatcccg cgtttgccgt tccctcagcc gataacagcc agctggtcat
     1861 cagtgcactg accgataagg ggttacggta tgcagggtat ggtgttgccg taaatcacga
     1921 tgcagacagc cacatatcac ctgcgccgga acttatggag tttcatctga aaacccgtga
     1981 ggcaccgctt tttgctgccg tcaacacacc agaaaaacag ccggatcatc tgttccgctc
     2041 tctgggtttc aatcgtacct gggatgaatg gcggcgcgag gaagatgcaa gaacacacac
     2101 caccgagagg agacatgatc gcggttggtc acagtgaatg tgaacaatat tttgttttgt
     2161 ttaacatcgt gttatgttcg tcgatgttct tctatgttca tcgacaggga tgaatgcaag
     2221 aagcacacac ataatgcaag catagttttg tttttccgcc cccaaaaatt gggggttctt
     2281 ttttcgctgc attagagcgc ttttgacgca atttttcact tcaatagtac cttcgcacta
     2341 ctaacacaaa aaaaacgcag cagggagcgt tttaggtgcc ttaaaatcga ttttagatcg
     2401 tcatcttgat ctgtttgtct tccttgaagc gaaaatgcag ggctacaacc gtgcgcccct
     2461 ttttgatcgt ctcaaccgtt acagccaggt cgcttttttt gttcagttcg tcaacacagg
     2521 gttttattag tattttattt aagtctctga attgtttgta tttattttca atccctaaca
     2581 ttgaacgaaa gtcattaaga gcaataattc tctctcccgt actgcggaat tgctggagga
     2641 actcgtatat tcttatactg taactacttg ataaacctga tatgtttttt ataacaacgc
     2701 gcgtgaattg ccccttcagt tgcgtcagat aaggcattac cgcgtcagaa aaggttattc
     2761 gagccttggc ctcaccttta gcgtactgtg cccgatactg gatccagcgg aactcctcac
     2821 gcttctcgtc attcttgaga atgatcgacc tgtcccataa acgatcgatc gcttcctgta
     2881 actgaacctc ggcatttttc cgccccatgt cagggaatga atcaaaatat tccgcagcgg
     2941 taatggtcat tgtcttttgc aattcagcat cagcaccaga tttcaatcga ctgatacaca
     3001 gcaacagaaa acgttgttcc tgtagggtca acttgtagct ggcctcaacc aaagcattcg
     3061 acttgtaggc aatcaagtcc gacatacaaa gatcccgttc aaatgtggtg atgctttaat
     3121 gtgccccaca aataagaaat gtggaatatt tttttttata tcctcacatt tctgtgaata
     3181 acaccttaaa ttcctccacc tttaccttaa attcctccac ctaaacacct taaattcctc
     3241 cacctttacc ttaaattcct ccacatatac ctctataaca tattgatttt attaatgaat
     3301 tttgacccta aaacaagtat taaaaacaag atcttaaaaa caagatctaa aaacttaacg
     3361 gcgagcctgt ggataactcg gggcgctgaa aacaaaaaac caataaaaac aatgtgttgc
     3421 aagaaagcag aattgcaagt tccgaatact ccccttccaa ccctgtggaa atccagtatt
     3481 catgcgggtt cagcgcatat cacctaccga aaaaccgtgc gtatgtgctc aacctggcga
     3541 cggattcact tccgcttcct cgcccactcg ctcgctaacg ctcggtcgct ggggctgcgg
     3601 cgagcggacg atacaatcac tgtcctggca gcgcctccag ctcgttaatc agcggcagta
     3661 tgcgcgtcac atcagcctgt cgtgtcacct ctgccttcag gaactggagt aaccaggcac
     3721 gcaactgcac ctcctccggc tttctgacct ttgccagcaa tgctgcaccc acaacaacct
     3781 tctgcgctgt gtccaggctc cggctcttcg ccttcaggcg ctgtaatctc gcctcagcat
     3841 cagcaatctt ctgttcgagc gttctgctca tgatgtaact ccgtgcgcga tgaaaaatcg
     3901 cattttagcg cgtcactggt agtttaaaaa ctgagctggc ataatgcacg acaaatcacg
     3961 aagtgcgcac ttatacaatc tccactccgt ttcgattgtg tgcgccctgc ggggctgaaa
     4021 gaaaactaca aaaaggcatt acggcagaaa tggcgattta tcatctcagc at
//
