LOCUS       NZ_CP135251             6647 bp    DNA     circular CON 07-OCT-2023
DEFINITION  Escherichia coli strain OHT76-1 plasmid pOHT76-1_7, complete
            sequence.
ACCESSION   NZ_CP135251
VERSION     NZ_CP135251.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37300944
            Assembly: GCF_032300135.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6647)
  AUTHORS   Wittum,T.
  TITLE     Salmonella and antimicrobial resistant bacteria recovered from
            retail pet treats in the US
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6647)
  AUTHORS   Li,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-SEP-2023) CVM/OAS, FDA-CVM, 8401 Muirkirk Rd, MOD2,
            Laurel, MD 20708, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-JUL-2021
            Assembly Method                   :: HGAP v. 4
            Assembly Name                     :: Microbial assembly
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 228x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/07/2023 07:18:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,262
            CDSs (total)                      :: 5,137
            Genes (coding)                    :: 4,891
            CDSs (with protein)               :: 4,891
            Genes (RNA)                       :: 125
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 93
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 95 of 246
            Pseudo Genes (incomplete)         :: 150 of 246
            Pseudo Genes (internal stop)      :: 47 of 246
            Pseudo Genes (multiple problems)  :: 42 of 246
            Pseudo Genes (short protein)      :: 2 of 246
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP135251.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="OHT76-1"
                     /isolation_source="Pig ear pet treat"
                     /host="Food"
                     /db_xref="taxon:562"
                     /plasmid="pOHT76-1_7"
                     /geo_loc_name="USA:OH"
                     /collection_date="2021-06-14"
                     /collected_by="The Ohio State University"
     gene            complement(join(6609..6647,1..399))
                     /locus_tag="RRJ52_RS26245"
                     /old_locus_tag="RRJ52_26245"
     CDS             complement(join(6609..6647,1..399))
                     /locus_tag="RRJ52_RS26245"
                     /old_locus_tag="RRJ52_26245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001405929.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="hypothetical protein"
     gene            659..2227
                     /gene="cea"
                     /locus_tag="RRJ52_RS26250"
                     /old_locus_tag="RRJ52_26250"
     CDS             659..2227
                     /gene="cea"
                     /locus_tag="RRJ52_RS26250"
                     /old_locus_tag="RRJ52_26250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="WP_000447006.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     gene            complement(2224..2565)
                     /locus_tag="RRJ52_RS26255"
                     /old_locus_tag="RRJ52_26255"
     CDS             complement(2224..2565)
                     /locus_tag="RRJ52_RS26255"
                     /old_locus_tag="RRJ52_26255"
                     /inference="COORDINATES: protein motif:HMM:NF015489.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="WP_000058760.1"
                     /translation="MSLRYYIKNILFGLYCTLIYIYLITKNSEGYYFLVSDKMLYAIVI
                     STILCPYSKYAIEYIAFNFIKKDFFERRKNLNNAPVAKLNLFMLYNLLCLVLAIPFGLL
                     GLFISIKNN"
                     /name="colicin E1 immunity protein"
     gene            2613..2750
                     /locus_tag="RRJ52_RS26260"
                     /old_locus_tag="RRJ52_26260"
     CDS             2613..2750
                     /locus_tag="RRJ52_RS26260"
                     /old_locus_tag="RRJ52_26260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001405930.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTIAPSSSSKLTGIAVQ
                     "
                     /name="colicin release lysis protein"
     gene            complement(2949..3107)
                     /locus_tag="RRJ52_RS26265"
                     /old_locus_tag="RRJ52_26265"
     CDS             complement(2949..3107)
                     /locus_tag="RRJ52_RS26265"
                     /old_locus_tag="RRJ52_26265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071531517.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRAMMA
                     QTVLQSS"
                     /name="replication initiation protein"
     gene            complement(3777..3968)
                     /locus_tag="RRJ52_RS26270"
                     /old_locus_tag="RRJ52_26270"
     CDS             complement(3777..3968)
                     /locus_tag="RRJ52_RS26270"
                     /old_locus_tag="RRJ52_26270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            4032..4355
                     /locus_tag="RRJ52_RS26275"
                     /old_locus_tag="RRJ52_26275"
     CDS             4032..4355
                     /locus_tag="RRJ52_RS26275"
                     /old_locus_tag="RRJ52_26275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4564..>4995
                     /locus_tag="RRJ52_RS26280"
                     /old_locus_tag="RRJ52_26280"
                     /pseudo=""
     CDS             4564..>4995
                     /locus_tag="RRJ52_RS26280"
                     /old_locus_tag="RRJ52_26280"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            5033..5551
                     /locus_tag="RRJ52_RS26285"
                     /old_locus_tag="RRJ52_26285"
     CDS             5033..5551
                     /locus_tag="RRJ52_RS26285"
                     /old_locus_tag="RRJ52_26285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067500.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            5558..5791
                     /locus_tag="RRJ52_RS26290"
                     /old_locus_tag="RRJ52_26290"
     CDS             5558..5791
                     /locus_tag="RRJ52_RS26290"
                     /old_locus_tag="RRJ52_26290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137445.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(6109..6528)
                     /locus_tag="RRJ52_RS26295"
                     /old_locus_tag="RRJ52_26295"
     CDS             complement(6109..6528)
                     /locus_tag="RRJ52_RS26295"
                     /old_locus_tag="RRJ52_26295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     Typing          3605..3691
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.43e-38"
     Typing          complement(3036..3591)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.699"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          4346..5895
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.473"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttccagttcc agtgcctgcc tgagctgggc tatctcattt tgtagctgtt cccgttctgc
       61 ttcctggcgt ctgcgatcca tatcctgtgc ctgtttatcc tcaagcatca gcgtcaggca
      121 ttgtttcagc tcattcagct cccggagaat gcgttctgtc tgctgatcat gtggattttc
      181 tgcatgtccc tcactgtgcc tttctggtgt ctcattctgc tttaattcgc cgtatgcccg
      241 gatcagttca ctggtttcaa actcccgtcg tccgtccctg ccggtacggt aactgacaag
      301 gcctgcgcgc atatcacggt aaaactgtga acgcgatctg cctgtcattt ttagtgcgtc
      361 ccggagtgtg tgccatgcca taaagtgaca gtgtcccata gatgtctcat ctcatagttt
      421 cagtaaaaca taatgaggtc tgagaacggt aatgtttgtg ctggtttttg tggcatcggg
      481 cgagaatagc gcgtggtgtg aaagactgtt tttttgatcg ttttcacaaa aatggaagtc
      541 cacagtcttg acagggaaaa tgcagcggcg tagcttttat gctgtatata aaaccagtgg
      601 ttatatgtac agtatttatt tttaacttat tgttttaaaa gtcaaagagg attttataat
      661 ggaaaccgcg gtagcgtact ataaagatgg tgttccttat gatgataagg gacaggtaat
      721 tattactctt ttgaatggta ctcctgacgg gagtggctct ggcggcggag gtggaaaagg
      781 aggcagtaaa agtgaaagtt ctgcagctat tcatgcaact gctaaatggt ctactgctca
      841 attaaagaaa acacaggcag agcaggctgc ccgggcaaaa gctgcagcgg aagcacaggc
      901 gaaagcaaag gcaaacaggg atgcgctgac tcagcgcctg aaggatatcg tgaatgaggc
      961 tcttcgtcac aatgcctcac gtacgccttc agcaacagag cttgctcatg ctaataatgc
     1021 agctatgcag gcggaagcag agcgtttgcg ccttgcgaaa gcagaagaaa aagcccgtaa
     1081 agaagcggaa gcagcagaaa aggcttttca ggaagcagaa caacgacgta aagagattga
     1141 acgggagaag gctgaaacag aacgccagtt gaaactggct gaagctgaag agaaacgact
     1201 ggctgcattg agtgaagaag ctaaagctgt tgagatcgcc caaaaaaaac tttctgctgc
     1261 acaatctgaa gtggtgaaaa tggatggaga gattaagact ctcaattctc gtttaagctc
     1321 cagtatccat gcccgtgatg cagaaatgaa aacgctcgct ggaaaacgaa atgaactggc
     1381 tcaggcatcc gctaaatata aagaactgga tgagctggtc aaaaaactat caccaagagc
     1441 caatgatccg cttcagaacc gtcctttttt tgaagcaacc agacgacggg ttggggccgg
     1501 taagattaga gaagaaaaac aaaaacaggt aacagcatca gaaacacgta ttaaccggat
     1561 aaatgctgat ataactcaga tccagaaggc tatttctcag gtcagtaata atcgtaatgc
     1621 cggtatcgct cgtgttcatg aagctgaaga aaatttgaaa aaagcacaga ataatctcct
     1681 taattcacag attaaggatg ctgttgatgc aacagttagc ttttatcaaa cgctgactga
     1741 aaaatatggt gaaaaatatt cgaaaatggc acaggaactt gctgataagt ctaaaggtaa
     1801 gaaaatcggc aatgtgaatg aagctctcgc tgcttttgaa aaatacaagg atgttttaaa
     1861 taagaaattc agcaaagccg atcgtgatgc tatttttaat gcgttggcat cggtgaagta
     1921 tgatgactgg gctaaacatt tagatcagtt tgccaagtac ttgaagatta cggggcatgt
     1981 ttcttttgga tatgatgtgg tatctgatat cctaaaaatt aaggatacag gtgactggaa
     2041 gccactattt cttacattag agaagaaagc tgcagatgca ggggtgagtt atgttgttgc
     2101 tttacttttt agcttgcttg ctggaactac attaggtatt tggggtattg ctattgttac
     2161 aggaattcta tgctcctata ttgataagaa taaacttaat actataaatg aggtgttagg
     2221 gatttaatta ttctttattg atataaaaag tcctagcaat ccaaatggga ttgctaggac
     2281 caaacaaagt agattatata gcataaatag gtttaatttt gctacggggg cgttatttag
     2341 gttttttctt ctttcgaaaa aatctttctt tatgaagtta aaagctatgt attcaatagc
     2401 atattttgaa tatggacata gaatagtgct tatcactatt gcatatagca tcttatctga
     2461 cacaaggaaa taataccctt cgctgttttt tgttataagg tatatatata taagtgtgca
     2521 gtacaggcca aataaaatat tttttatgta gtatcttaag ctcataaatt aaacctcgcc
     2581 atatattctt ttcattttat aaggatcgag ttatgaggaa aagatttttt gtgggaatat
     2641 tcgcgataaa cctccttgtt ggatgtcagg ctaactatat acgtgatgtt cagggaggga
     2701 ccatcgcacc atcctcctct tctaaactga cggggatcgc ggttcagtag aaaagatcaa
     2761 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     2821 accgctacca acggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     2881 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagtcggg
     2941 ccactacttc aagaactctg tagcaccgtt tgtgccatca tcgctctgct aatccggtta
     3001 ccagtggctg ctgccagtgg cgttaaggcg tgccttaccg ggttggactc aagacgatag
     3061 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     3121 gagcgaacga cctacaccga actgagatac caacagcgtg agctatgaga aagcgccacg
     3181 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     3241 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     3301 cacctctgac ttgagcgtct atttttgtga tgctcgtcag gggggcggag cctatggaaa
     3361 aacgcctgct acgtggcctt cttcctgttc ctggtctttt gctcacatgt tctttccggc
     3421 cttatcccct gattctgtgg ataactgtgt taccgttttt gtgtgagtca gtaccgctcg
     3481 ccgcagtcga acgaccgagc gtagcgagtc agtgagcgag gaagcggaaa agcgcctgga
     3541 cgtgcatttt ctccttacgc atctgtgcgg catttcacac ccggcatggc gtacttttca
     3601 tacaatccgc actgatgccg catggttaag ccagtataca ctccgctatc gctacgtgac
     3661 tgggtcatgg ctgcgccccg acacccgcta aaacccgctg acgcgccctg acgggcttgt
     3721 ctactcccgg tatccgctca caggcaagct gtgaccgtct ccgggagctg tatgtgtcag
     3781 aggttttcac cgtcatcccc gaagcgtgcg aggcagctgc ggtaaagctc atcggcgtgg
     3841 tcgtgaagcg attcacaaat atcggcctgt tcatctgcgt ccagttcgtt gagcttctcc
     3901 agcagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt
     3961 ttagtcactg atgcctccgt gtaaggggga tttctgttca tggggtaatg ataccgatga
     4021 aacgcgagag gatgctcaca atacgggtta ctgatgatga acatgcccgg ttacttgaac
     4081 gctgtgaggg taaacaactg gcggtatgga tgcggcgggt ctgcctgggg gagccggttg
     4141 cccgttccgg aaaactgccg acactggcac cgccgttact gcgtcagctg gccgccatcg
     4201 gaaataacct gaatcagaca gcccgtaagg tgaacagcgg gcagtggtct tccggtgacc
     4261 gggttcaggt ggtggccgca ctgatggcca tcggggatga gctgcgccgg ctgcgtctgg
     4321 ctgtcaggga acagggggcg cgggatgata gttaaatttc atgccagggg aaaaggtggt
     4381 ggcagtggtc cggttgatta cctgctgggg agggagcgta accgcgaagg cgcaacggtg
     4441 cttcagggta atccggaaga agtccgggaa ctcatcgatg ccacgccatt tgcgaagaaa
     4501 tacacgtccg gtgttctgtc gttcgcggag aaggagctgc cgccgggagg acgtgaaaaa
     4561 gtgatggcga gctttgagcg tgtactgatg cccggtctcg aaaaaaatca gtacagcatc
     4621 ctgtgggtgg agcaccagga caagggacgg cttgagctga attttgtcat tccgaacatg
     4681 gagctacaga ccggaaaacg cctccagccg tactacgacc gcgcagacag gcctagaatt
     4741 gatgcctggc agacgctggt gaatcaccat tacgggctgc atgacccgaa cgccccggag
     4801 aaccgcagga cgctgacact ccctgataac ctgcctgaaa cgaaacaggc gcttgctgag
     4861 ggcgtcacgc gaggtataga tgcactttac catgccggag agataaaagg ccgtcaggat
     4921 gtgattcagg cgctcactga ggcggggctg gaagtggtca gggtgacgcg aagcagtatc
     4981 agcattgcag atccgaacgg cgggaagaat atcaggctga aaggagcatt ttatgagcaa
     5041 tcttttgcag acgggcgcgg agttcgagaa aaagctgaaa gagagagccg aatctacaga
     5101 gaaaatgctg aacaacgagt tcaggaggct cggcgaatct gtaagcgagg ctgtgacatc
     5161 aaacgagacg aaaatcagag acgctatagc cctgttcaca gcctcgacag aggaatcgct
     5221 ggaaaaacac cgggaagggg tgaaagaggc gatgatgcag cacaggaggg acgtgttaaa
     5281 gctggcaggg aatacgggca tgatgttact ggggatagtc tttctcctgt ttaccgcgag
     5341 tggcgggacg ctctggtatc ttggagggag gatacaggcg aacctggaag aaatcaggaa
     5401 gcaggaagag acattgcaga aactgaacgc gaagacatgg ggcgtggagt ttgtgcagga
     5461 cgggaacagg aaattccttg tccttccgta cgggaaatca gcggaggtga ttccctttca
     5521 ggggaaagag tgggtacatc tgaaggagtg acacagagtg acagagctgg aaacacattt
     5581 gctgaacgcc ttagagcagc tgcaacagga ctatatgcag cggctgagcg aatgggagag
     5641 cgccttcgtg gaattgcaga agatgttttc gcttacgcaa cgggacaacg cgatgctgaa
     5701 cgagcgggtc atgcagttga gtcagcaggt gcagcacttg agcgagcaga cagaacgctt
     5761 gagccagtta tacagcgaga actggagata agagaggaac ggctgataca ggagcgcgaa
     5821 catgtgttat ccctggaacg ggagcgtcag ccggaaatac aggaacgcac gctggatggc
     5881 ccttcgctgg gatggtgaaa ccatgaaaaa tggcagcttc agtggattaa gtgggggtaa
     5941 tgtggcctgt accctctggt tgcataggta ttcatacggt taaaatttat caggcgcgat
     6001 cgcggcagtt tttcgggtgg tttgttgcca tttttacctg tctgctgccg tgatcgcgct
     6061 gaacgcgttt tagcggtgcg tacaattaag ggattatggt aaatccactt actgtctgcc
     6121 ctcgtagcca tcgagataaa ccgcagaaat cgtgtcagcc agcagcctgg attttttcgg
     6181 ggtaaggtct ttgctgcggt aatcagtacc cgcaaattga gttgtgcccc ggagtgagtt
     6241 taaaaattcc tggctgtgaa actgctgaat gcgttgttgc acatcactcc gtgattttcc
     6301 tgtcgcttta tctttagctc ccatatccca gaactgccga aaaaaaggca ccatcattcg
     6361 tgttgtgtcg gctttttggg tggaaaagtt gggatcaaaa ccgtcatcag aagcatatac
     6421 gtaatgccgg gtatgttgtt ccacgctgcc ctttggggta caggctgtga gagctactgt
     6481 tgctgttatc atggcgataa gacaaagttt gtttccggta cgtctcatga gtgtctccct
     6541 accttaaagt atttattctc ggtgagttat tatccgaaga ctattttatc atcagtctct
     6601 gacagcgttc agcgaccgaa caacctggac cagaatcccc gtttctt
//
