LOCUS       NZ_CP135250             7098 bp    DNA     circular CON 07-OCT-2023
DEFINITION  Escherichia coli strain OHT76-1 plasmid pOHT76-1_6, complete
            sequence.
ACCESSION   NZ_CP135250
VERSION     NZ_CP135250.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37300944
            Assembly: GCF_032300135.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7098)
  AUTHORS   Wittum,T.
  TITLE     Salmonella and antimicrobial resistant bacteria recovered from
            retail pet treats in the US
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7098)
  AUTHORS   Li,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-SEP-2023) CVM/OAS, FDA-CVM, 8401 Muirkirk Rd, MOD2,
            Laurel, MD 20708, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-JUL-2021
            Assembly Method                   :: HGAP v. 4
            Assembly Name                     :: Microbial assembly
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 228x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/07/2023 07:18:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,262
            CDSs (total)                      :: 5,137
            Genes (coding)                    :: 4,891
            CDSs (with protein)               :: 4,891
            Genes (RNA)                       :: 125
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 93
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 95 of 246
            Pseudo Genes (incomplete)         :: 150 of 246
            Pseudo Genes (internal stop)      :: 47 of 246
            Pseudo Genes (multiple problems)  :: 42 of 246
            Pseudo Genes (short protein)      :: 2 of 246
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP135250.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7098
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="OHT76-1"
                     /isolation_source="Pig ear pet treat"
                     /host="Food"
                     /db_xref="taxon:562"
                     /plasmid="pOHT76-1_6"
                     /geo_loc_name="USA:OH"
                     /collection_date="2021-06-14"
                     /collected_by="The Ohio State University"
     gene            complement(join(5480..7098,1..601))
                     /gene="mobQ"
                     /locus_tag="RRJ52_RS26210"
                     /old_locus_tag="RRJ52_26210"
     CDS             complement(join(5480..7098,1..601))
                     /gene="mobQ"
                     /locus_tag="RRJ52_RS26210"
                     /old_locus_tag="RRJ52_26210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_025672425.1"
                     /translation="MAIYHLSMKIISRKNGYSAVASAAYRSGSVIPDDRTGLTHDYTRK
                     CGVDDAVILTPANAPSWCGDRSVLWNAVEKAEQRRNSQLAREIELAIPREISREAARET
                     VLAFVRENFVSRGMIADVAFHHMDRTNPHAHIMLTTRAVGETGFAGKVRDWNDRALAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKAPGLHLGKAACAMEKRGMETERGEQ
                     NRLINSLNLEIQVSRTQLALRTVQEAQRKRELSDAARRAAEALNLTIPAANASADTLRE
                     FIATLPQECGNAWEMTPEFLAMSGKVNDIEREGNALLKEQAILEKEMTGLKKARPVASL
                     LSEIPLMTWAEPEYRKRQLRFWKLGKQIESLRRTYRAVKERDIPARRQAFETQWNTWIA
                     PGMAELKEKLSAREAERRREEAEAEARRKEQEHEARLKRHDNHRLSRETALVGVITELG
                     RAREPGTGRITRYMMLSNRAGEFTVWGDELAHYPRSVHDPVNVYLSPGGAVMVTDIHAG
                     RPESHETMARPERVRMYSGATVRHVLEQMRQGWPSYGFPALPHHWPDNFYFSNDRRPVA
                     SPLPSAHRVDITADAAPEQLMPVVFSREGNSRTLNLLLCKGPEEMLVGFVRREGGFCPV
                     LVLPSPDYSHLMVSTITENGVCLAGYGEAINHDEDTPYPPEPHLMQFRLKGHHDRLLAA
                     VHKPEEMPDYLFRQLGFNQTWHEWKRDEQHRQQQRRPGHFRGMSM"
                     /name="mobQ"
     gene            780..1055
                     /locus_tag="RRJ52_RS26215"
                     /old_locus_tag="RRJ52_26215"
     CDS             780..1055
                     /locus_tag="RRJ52_RS26215"
                     /old_locus_tag="RRJ52_26215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000091753.1"
                     /translation="MSRTLEQKIAEAEARLQRLKAKSRSLDTAQKVIVGAAMLARVRRP
                     EEAQLRAFLLQFLRKEVTRQADVNRLQPLINELEMLPRPPAKPQNH"
                     /name="hypothetical protein"
     gene            complement(1271..2164)
                     /locus_tag="RRJ52_RS26220"
                     /old_locus_tag="RRJ52_26220"
     CDS             complement(1271..2164)
                     /locus_tag="RRJ52_RS26220"
                     /old_locus_tag="RRJ52_26220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001277133.1"
                     /translation="MSAVLQRFREKLPHKPYCTNDFAYGVRILPKNIAILARFIQQNQP
                     HALYWLPFDVDRTGASIDWSDRNCPAPNITVKNPRNGHAHLLYALALPVRTAPDASASA
                     LRYAAAIERALCEKLGADVNYSGLICKNPCHPEWQEVEWREEPYTLDELADYLDLSASA
                     RRSVDKNYGLGRNYHLFEKVRKWAYRAIRQGWPVFSQWLDAVIQRVEMYNASLPVPLSP
                     AECRAIGKSIAKYTHRKFSPEGFSAVQAARGRKGGTKSKRAAVPTSARSLKPWEALGIS
                     RATYYRKLKCDPDLAK"
                     /name="replication initiation protein"
     gene            complement(2693..2836)
                     /locus_tag="RRJ52_RS26225"
                     /old_locus_tag="RRJ52_26225"
     CDS             complement(2693..2836)
                     /locus_tag="RRJ52_RS26225"
                     /old_locus_tag="RRJ52_26225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_021553018.1"
                     /translation="MKKITGIILLLLAVIILAACQANYIRDVQGGTVSPSSTAELTGVE
                     TQ"
                     /name="colicin release lysis protein"
     gene            complement(2898..3158)
                     /locus_tag="RRJ52_RS26230"
                     /old_locus_tag="RRJ52_26230"
     CDS             complement(2898..3158)
                     /locus_tag="RRJ52_RS26230"
                     /old_locus_tag="RRJ52_26230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012817927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_025672426.1"
                     /translation="MELKKSISDYTEAEFLELVKKICRAEGATEEDDNKLVREFKRLTE
                     HPDGSDLIYYPRDDREDSPEGIVKEIKEWRAANGKSGFKQG"
                     /name="bacteriocin immunity protein"
     gene            complement(3325..3582)
                     /locus_tag="RRJ52_RS26235"
                     /old_locus_tag="RRJ52_26235"
     CDS             complement(3325..3582)
                     /locus_tag="RRJ52_RS26235"
                     /old_locus_tag="RRJ52_26235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_025672427.1"
                     /translation="MGLKLHINWFDKTTEEFKGGEYSKDFGDDGSVIERLGMPLKDNIN
                     NGWFDVIAEWVPLLQPYFNHQIDISDNEYFVSFDYRDGDW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(3592..5247)
                     /locus_tag="RRJ52_RS26240"
                     /old_locus_tag="RRJ52_26240"
     CDS             complement(3592..5247)
                     /locus_tag="RRJ52_RS26240"
                     /old_locus_tag="RRJ52_26240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_025672428.1"
                     /translation="MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNP
                     WGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA
                     VSISAGALSAAIADIMAALKGPFKFGLWGVALYGVLPSQIAKDDPNMMSKIVTSLPADD
                     ITESPVSSLPLDKATVNVNVRVVDDVKDERQNISVVSGVPMSVPVVDAKPTERPGVFTA
                     SIPGAPVLNISVNNSTPAVQTLSPGVTNNTDKDVRPAGFTQGGNTRDAVIRFPKDSGHN
                     AVYVSVSDVLSPDQVKQRQDEENRRQQEWDATHPVEAAERNYERARAELNQANEDVARN
                     QERQAKAVQVYNSRKSELDAANKTLADAIAEIKQFNRFAHDPMAGGHRMWQMAGLKAQR
                     AQTDVNNKQAAFDAAAKEKSDADAALSSAMESRKKKEDKKRNAENKLNEEKNKPRKGVK
                     DYGHDYHPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKGRKIYEWDSQHGELE
                     GYRASDGQHLGSFEPKTGNQLKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     BGC             1..7098
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          244..602
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.889"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          603..777
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="7.17e-87"
     Typing          complement(1272..2164)
                     /name="rep_cluster_2150"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.163"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3..601)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.0"
                     /coverage="99.0"
                     /evalue="8.52e-122"
     Typing          complement(5484..7096)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.513"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ggcccggcgc tttctccagt ccctgacgct cgtatgaacg atggtctatc tcttcctggt
       61 agccggcgtt cgcaagcgct ctgttcgcat ggtcagccca tgatgcgcgc cacgtctcgg
      121 cgagtgcccg gtcgttccag tccctgacct ttcctgcgaa tcccgtttcc ccgacagctc
      181 tcgtggtcag catgatgtgc gcatggggat tggtccggtc catgtgatgg aacgccacat
      241 cggcaatcat gccccgactg acaaagtttt cccggacaaa agcgagaacg gtctcccgtg
      301 cggcctcgcg ggaaatctca cggggaatgg caagttcaat ctcccttgcc agctgggagt
      361 tccggcgctg ttcggctttc tcgaccgcat tccagagaac ggaacggtca ccacaccagg
      421 acggtgcatt cgcaggggtg agaatgaccg catcatcaac gccgcattta cgggtgtaat
      481 cgtgggttaa tccggtacgg tcatcgggta tgacagagcc ggaacggtag gcagcagaag
      541 caacggcact gtagccgttt tttcgcgaaa tgattttcat gctgagatga taaatcgcca
      601 tttctgccgt aatgcctttt tgccgttttc ttttagcgtc gcagaccgca cacaatcgaa
      661 acgaagtgga gattgtataa gtgcgcactt cgtgatgtgc cgtgcattat gccagttcag
      721 ttttgcaact accagtgacg cgctaaaatg cgatttttca ccgcgcatgg agtcacaaaa
      781 tgagcagaac actcgaacag aagattgccg aagctgaggc cagattacag cgcctgaagg
      841 cgaagagccg gagcctggac acggcgcaaa aagtcattgt gggggctgcc atgctggcga
      901 gggtacggag gccggaagaa gcgcaactgc gtgcgttcct gctccagttt ctgcgaaagg
      961 aagtcacaag gcaggcggat gtgaaccgac tacaaccgct gattaacgag ctggagatgc
     1021 tacccagacc acccgcgaaa ccgcagaacc actaagcccc ggcgcgtaag tgcccatgca
     1081 ccaccggacg cctttgcgct tcctcgctca ttcgctcgct acgctcggtc atgagactgc
     1141 ggcgagcgct accacctcaa tcatgcggaa agccacgcca ttacggggca gaacggagaa
     1201 aatcgcaaga aagatcacag ctcaaaaaga cgccaaaagg gcgctgttat ctgataaggc
     1261 ttatctggtc tcattttgcg agatctggat cacattttaa ttttcggtag tacgtcgctc
     1321 gactgatccc taatgcctcc cacggcttca gcgaacgtgc tgatgtagga actgctgcgc
     1381 gcttagattt agttccgccc ttgcgacctc gagcggcctg tacagcggaa aatccctctg
     1441 gtgagaattt cctgtgtgta tatttcgcaa tgctcttgcc aatagcccga cattcagccg
     1501 gagaaagcgg aacgggaagc gaggcgttgt acatttcgac acgctggatc acggcatcaa
     1561 gccattgtga gaatacaggc cagccctgac gaatcgctct gtaggcccat ttacggacct
     1621 tttcgaacag atggtagttt cgccccagcc cgtaattttt atcgacgcta cggcgcgctg
     1681 aggcgctcaa atcgagataa tcagccagtt cgtcgagagt gtagggttcc tcgcgccatt
     1741 ccacttcctg ccattcaggg tggcacggat ttttgcagat caggccgctg taattcacat
     1801 ccgcgcccag tttttcacac aacgcacgct caatagcggc agcgtatctg agcgccgaag
     1861 ccgatgcatc cggcgcagtt ctcacaggaa gggcgagcgc gtagagcaga tgcgcgtgcc
     1921 cgttacgggg attttttacg gtgatgttcg gggccggaca attccggtcg ctccagtcga
     1981 ttgatgcccc cgtccggtcc acgtcaaagg gaagccagta cagtgcatgt ggctggttct
     2041 gctggatgaa acgggcaaga atggcaatgt ttttcggcag aatgcgaacg ccgtacgcga
     2101 aatcgttcgt acagtacggt ttgtgcggta atttttccct gaagcgttga agtacggcgc
     2161 tcatgtgata cgatttctct cggtacacag tttaactaac cgcgctttcc catcgggaaa
     2221 cgcggaaccc ggcgccctgt aatttcgtga tcaatcttgg cggtgagagc acgatttacg
     2281 gggcgtttgt ttttcagtcg cttacactac acccaacggc aaatcctgtc gaaccttccc
     2341 gaaaacccga tccatttacc ataactcccg ttgtacgcac cccttaaaac accctcagtc
     2401 gctttctgac gcaaaaaaaa caaaaagaca aggaaacacc agggtaaaca taaaaaatgc
     2461 gtcagaatcg tttttagcgc gttgcataac tatgcacgaa agcgatgcac gatcggtttc
     2521 gtgcgttctg aacaccgaac ggctcaaaac gacaagtttt ctccccctgg atggaaagtt
     2581 ctgcacgggg attttagatc ccatctccca catggaattt ttgtgacatt tcaaaaaaaa
     2641 gcgcatcaga atcagcctga cacgctttgt ttttgtcaaa gaggatttcg ggttactgcg
     2701 tttccactcc ggtcagttca gcagttgacg acggtgatac agtcccgccc tgaacatcac
     2761 ggatatagtt tgcctgacat gcagccagaa taatgactgc aagaagcaat aaaataatcc
     2821 ctgttatttt tttcataacg actccttgtt gtgaaagaat gccagtcatt catccttaaa
     2881 caaccggcat tcatatttca gccctgttta aatcctgact taccgttagc agctcgccat
     2941 tctttaattt ccttgacaat cccttcagga ctatcttccc tgtcatcgcg aggataataa
     3001 atcagatctg aaccatctgg gtgctcagtt aatcgcttaa actctctcac taatttattg
     3061 tcatcctctt cagtagcacc ttcagctcta catatttttt ttacaagctc cagaaattca
     3121 gcctcggtat aatcactaat acttttcttt agctccacct tcacctctcc tgagaatgtt
     3181 ttaaataatg cctatttaaa ttattgcaga gcaattataa ccaagaactc aataaaaaat
     3241 ataacacttc tacatttgga ggctccagcg ttcatccatg aacactagaa gcccgtacat
     3301 caactcatcc ctgataatat ttgatcacca atcaccatca cgataatcaa acgaaacaaa
     3361 atactcatta tcggaaatat caatttgatg attaaagtat ggttgtagca aaggtaccca
     3421 ttcagctata acatcaaacc aaccattatt gatattatct tttaaaggca ttccaagacg
     3481 ttcaatgacc gagccatcat ctccaaaatc ttttgaatac tcaccacctt taaattcctc
     3541 ggtcgtctta tcaaaccaat taatatgtaa tttaagcccc ataacatcct ctcaaagata
     3601 ctttttgata tttcgttttg gatcaggtcc tttcaactga ttaccagtct taggctcgaa
     3661 tgagccaaga tgctgaccat cactggcacg atacccctca agctcaccat gctgagagtc
     3721 ccactcataa atcttacgcc ctttatctcc ataccatcta gcacgtttac cgccaccacc
     3781 ttgctttgga gtttttggtt taccctcttt taactcaccc agccctttta tatcttcagt
     3841 tttaggatct ggatgataat catgaccgta atctttaact cccttgcgag gcttgttttt
     3901 ttcctcgttt aatttatttt cagcgttcct tttcttatct tctttcttct tcctgctttc
     3961 catagcagaa ctcaatgcag catcagcatc tgacttctct tttgcagcag catcaaatgc
     4021 agcctgctta ttatttacat ccgtctgcgc ccgctgagct ttaagcccgg ccatttgcca
     4081 cattctgtga ccgccagcca ttgggtcatg tgcaaatcga ttaaattgtt ttatttcagc
     4141 tattgcatca gcaagagttt tattcgctgc atcaagttcg cttttacgcg aattataaac
     4201 ctgaacagct ttagcctgtc gctcctgatt tctggcaaca tcttcatttg cctgattcag
     4261 ctctgcacgc gcgcgttcat aatttcgctc agccgcttca accggatgcg tagcatccca
     4321 ttcctgctga cggcgatttt cttcatcctg acgttgtttt acctggtcag ggctaagaac
     4381 atcactcact gaaacatata cggcattatg accgctgtcc ttcgggaatc gaataactgc
     4441 atccctggta ttaccaccct gagtaaatcc tgccgggcga acatccttat cagtattatt
     4501 tgtaacacct gggcttaatg tctgtactgc tggcgtactg ttattaactg aaatattcag
     4561 aacaggtgca cctggaattg atgccgtaaa aacacctgga cgttcggtag gttttgcatc
     4621 aaccaccgga acactcatcg gaacacctga aacaaccgaa atattctgtc gttcgtcttt
     4681 tacatcatca acaacacgaa catttacgtt tactgttgcc ttatcgagag gtaatgaact
     4741 gacaggtgat tcagtaatat catctgcggg taatgacgtc acaatctttg acatcatatt
     4801 ggggtcatct ttcgctattt gtgatggcaa tacaccatat aaagccaccc cccaaagacc
     4861 aaatttaaac ggtcctttca gggcagccat aatatcagca atagctgccg ataatgctcc
     4921 cgctgaaata ctgaccgcca gaccgccagc tcctggagtg gaaagtgccg gaaaaccaaa
     4981 tgccactggc gcagctactg ctgacagatt accgcctgtt cccgagccac caccggaatt
     5041 accattcccc ccgccattac catgaccgga accaccaccc cagtgaatgc cgctaccgga
     5101 accaccaccc cacgggttat tttccgaact ccacccggaa ccatcagaag caccaccacc
     5161 tacaccaagc ccggtcgggc caccattaat gttaccactt gtgctatgcg cgcccgtgtt
     5221 atggccgcgt ccatcgccac cgctcataaa aactccctct tttaagcgtt aaaacaatta
     5281 attacatgat taatactgta cataaaacca ctggttttat gtacagatca aaaagtaccc
     5341 gtcatcgttt tttctgtcaa gttcaggtgg aaaatttttt agaaatcagg agggataaca
     5401 cttagaaaac gcagagcggg ccacaaaaaa ttttgtggcc cgtgatgtgt tggcggggtc
     5461 cggggtgcag ccccggatat cacatactca tgccccggaa gtgcccgggg cggcgttgtt
     5521 gttgcctgtg ctgttcgtcc cgcttccact catgccaggt ctgattaaaa ccgagttgac
     5581 ggaagagata atccggcatc tcttccggtt tgtggacagc agccaaaagc ctgtcatgat
     5641 ggcctttgag ccggaactgc atcaggtgcg gttccggtgg gtacggagta tcctcatcat
     5701 ggtttatagc ttctccgtaa cctgccaggc ataccccgtt ctccgtgatg gtgctgacca
     5761 tcagatgact gtaatccggc gacggaagca caagaacggg gcaaaaccct ccttcccggc
     5821 gaacaaaccc gacaagcatt tcctccggcc ctttgcacaa caacagattc agcgtcctgc
     5881 tgtttccctc tctcgaaaat acaacaggca tgagttgttc cggtgccgca tcagcggtga
     5941 tatccacccg gtgcgctgac ggtagcggag aggctacggg cctgcggtcg ttgctgaaat
     6001 aaaaattatc cggccagtga tgcggcagcg ccggaaaacc gtaagagggc cacccctggc
     6061 gcatctgttc cagtacatgc cggaccgtcg caccggaata cattctcaca cgctcaggcc
     6121 gcgccattgt ctcatgagat tcaggccgcc ctgcgtgtat atccgtgacc atcacagccc
     6181 cgccaggcga cagataaaca ttcaccgggt catgaacact ccgggggtaa tgcgccagct
     6241 catcacccca taccgtgaat tctccggccc tgttactcag catcatgtag cgggttatcc
     6301 tgcccgttcc cggctctctg gcacgcccca gctccgtaat aaccccgact aatgccgttt
     6361 cacggctcag acggtggtta tcatgacgtt tcagccgcgc ctcatgctcc tgttccttcc
     6421 ggcgcgcttc cgcttcggct tcctccctgc gccgctccgc ttcccgtgct gacagttttt
     6481 ctttcagctc tgccattccc ggcgcaatcc acgtattcca ctgcgtttca aaggcctgac
     6541 gacgggcggg aatgtcccgt tctttcacgg ccctgtaggt gcggcgaaga gattcaatct
     6601 gtttcccgag tttccagaaa cggagttgtc ttttgcggta ttccggttca gcccatgtca
     6661 tcaggggaat ctctgacaga agggacgcga cagggcgtgc ttttttgagt ccggtcatct
     6721 ccttttcgag aatggcctgc tctttcagca gcgcattccc ctcacgttcg atgtcgttca
     6781 ccttcccgct catcgccagg aactccgggg tcatctccca cgcgttcccg cattcctgcg
     6841 gcagcgtggc aatgaattcc cgcagggtat ccgctgaggc attcgcagcg ggaatggtca
     6901 ggttaagggc ttccgctgca cgacgtgcag catcgctgag ttcccgctta cgctgcgctt
     6961 cctgaaccgt cctgagagca agctgcgtgc gggaaacctg tatttccagg ttaaggctgt
     7021 taatcagacg gttctgctca ccgcgttctg tttccattcc ccgtttttcc atcgcacagg
     7081 cagcctttcc gaggtgaa
//
