LOCUS       NZ_CP135229             2149 bp    DNA     circular CON 07-OCT-2023
DEFINITION  Escherichia coli strain Escherichia coli EC16611 plasmid pEC16611-4,
            complete sequence.
ACCESSION   NZ_CP135229
VERSION     NZ_CP135229.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37528253
            Assembly: GCF_032295145.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Peirano,G. and Pitout,J.
  TITLE     Genomic sequencing of MDR E. coli
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Peirano,G. and Pitout,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-SEP-2023) HMH-CDI, Hackensack-Meridian Health Center
            for Discovery and Innovation, 111 ideation way, Nutley, NJ 07110,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-SEP-2023
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Oxford Nanopore MiniION and Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/07/2023 07:08:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,115
            CDSs (total)                      :: 5,007
            Genes (coding)                    :: 4,787
            CDSs (with protein)               :: 4,787
            Genes (RNA)                       :: 108
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 220
            CDSs (without protein)            :: 220
            Pseudo Genes (ambiguous residues) :: 0 of 220
            Pseudo Genes (frameshifted)       :: 80 of 220
            Pseudo Genes (incomplete)         :: 154 of 220
            Pseudo Genes (internal stop)      :: 40 of 220
            Pseudo Genes (multiple problems)  :: 48 of 220
            Pseudo Genes (short protein)      :: 3 of 220
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP135229.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Escherichia coli EC16611"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC16611-4"
                     /geo_loc_name="Canada"
                     /collection_date="2016"
     gene            complement(585..851)
                     /locus_tag="RRI67_RS00010"
                     /old_locus_tag="RRI67_00010"
     CDS             complement(585..851)
                     /locus_tag="RRI67_RS00010"
                     /old_locus_tag="RRI67_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024536.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     gene            complement(855..1820)
                     /locus_tag="RRI67_RS00015"
                     /old_locus_tag="RRI67_00015"
     CDS             complement(855..1820)
                     /locus_tag="RRI67_RS00015"
                     /old_locus_tag="RRI67_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001287824.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     Typing          complement(1196..1427)
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 acccccgaac ggcttacccc aaccttttcc acacacccga tcaccaaaac agggaataaa
       61 aacaaaaccc tgtcttacag gtggatggcc tcacttcgtg aaaccatcca ccccgcataa
      121 gttttttaat cggtggtgtc gtcaatcact aaatcggaac cccaaagggg acccccacga
      181 tttagcattt gacgagatta actctcggag atggcatcat gcttttaacg gactttaaaa
      241 aacggtgaac ggatgcggat tttaatccgt gtctgtgagg cgttgagcaa cgagcaaagc
      301 gaggcggtag aaagagagcc agcggcaggc tcaggacgat aatccgggga aaatgtacca
      361 ctcgcagagt gttaacattt gacatcggat tgtctatcct gcattggtgt cgagttggca
      421 aggctgagga taatgctgag gataatgctg aggataatgc tgaggataat gctgaggata
      481 atgctgagga taatgctgag gataatgcag gatagacaga gcactagaga ttgcatgcaa
      541 tctcgtacct atcacctcag cctgccgctg gctcttttcc agaactaaga atgcgttctt
      601 aaaaatccaa acttgatacc gtttaaatct attttcacaa aaagagtttt taccctctcg
      661 tttgttttct gaatatataa aattaattca tcctcaaaac cttcgtgcgg atgaaaacca
      721 aatgtgccaa aacttaatcc atcaataagc aaaataaggt ttttaaacct gtcaggaatc
      781 accgcttcac ttcttaaaaa aaataaacca atagtcttat ttccattcag gtattcaata
      841 ttaatattca tacgttaatc cgatttatcc ttctcagggg cgcgtttgta acgccgttta
      901 cctgaatccc agacaaacgc cgtccgcttc ccgtcatcag tgccatcccc tacatcgtcg
      961 gcaatgacaa gatcctcatt ggtttctcga tccaactgga ggacgttttt tagcgcccca
     1021 ccggtcgaga taaaacggcg cttgtgaagc tgccgcgtca gctcaagaaa ccactcagga
     1081 tcgtttgcca tatcttccgg tttaacggag tatttcagcg tttcagcaac cgcgctttgc
     1141 agttgctcgg caacgttggc cacaacctca cctgtcttag tttttactgc ccgaatatcg
     1201 atattcggct catagttcac ccgcaagcaa tcgcgccaga gttctaccca acgttcgtgc
     1261 ttaacgtagt tcttcccttt aaaccaagaa ggttgcacca tcagcagaca gtgaaaatgc
     1321 ggatgtgcgc tgccatcctt acctcgcgtc acctccgtag ccctgatcca cccctgaaca
     1381 ggtgatagct cctttcgctt ttccattcgc ttaaacgccg cattcattgc tgtaaggact
     1441 gttccaagtt cacctatctc gcagttcctg acagttaacg tcagaaacaa ccatcgggaa
     1501 gacgggtaat ccacaacgat tttcggtaga gcctgataaa aacgggcttg ccacatgagg
     1561 gttcttctcc actggcagac agggcaatga cggacacggc aaaattgcgc actacgaagc
     1621 cgtaggcgcg tttctccggt ttccatgatg gtagaccaac caaaacgtaa caaaccgcta
     1681 caggaggcca tacggagtgc atagcgctcg aattcagcag cagtctggta gatacccccg
     1741 acagaatcgg taagcgtgcg ctgggtatcc caaactgcat cacgggggct gtagtccgaa
     1801 aggaatttat cttctgacat taagcctgct ccgatctggg caggctgtga tttgatgtgg
     1861 tatattcatc acgtacctgt gcattgcgag ccagtacatg tcaccagagt ttgccgctga
     1921 ggtgacacat tcgaaatgtt ggggagtgtt gacgcactcc ccaacgccga ttaaaaacgc
     1981 ctcaattctg ctccattttc tttgacctgt caaaacccac tcaaagcctc gtcattactg
     2041 ggctaaacca ctatttcata tccgccattt atcccttaag tataaataca aggatcacgc
     2101 gcgagaattg cgcaaaaaat aaccacaatt ttaacccccc aaaacaagc
//
