LOCUS       NZ_CP135222             2113 bp    DNA     circular CON 07-OCT-2023
DEFINITION  Escherichia coli strain Escherichia coli EC16409 plasmid pEC16409-5,
            complete sequence.
ACCESSION   NZ_CP135222
VERSION     NZ_CP135222.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37528252
            Assembly: GCF_032295845.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Peirano,G. and Pitout,J.
  TITLE     Genomic sequencing of MDR E. coli
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Peirano,G. and Pitout,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-SEP-2023) HMH-CDI, Hackensack-Meridian Health Center
            for Discovery and Innovation, 111 ideation way, Nutley, NJ 07110,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-SEP-2023
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Oxford Nanopore MiniION and Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/07/2023 07:47:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,050
            Genes (coding)                    :: 4,821
            CDSs (with protein)               :: 4,821
            Genes (RNA)                       :: 115
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 87 of 229
            Pseudo Genes (incomplete)         :: 159 of 229
            Pseudo Genes (internal stop)      :: 37 of 229
            Pseudo Genes (multiple problems)  :: 51 of 229
            Pseudo Genes (short protein)      :: 4 of 229
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP135222.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Escherichia coli EC16409"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC16409-5"
                     /geo_loc_name="Canada"
                     /collection_date="2016"
     gene            join(1625..2113,1..477)
                     /locus_tag="RRI70_RS00005"
                     /old_locus_tag="RRI70_00005"
     CDS             join(1625..2113,1..477)
                     /locus_tag="RRI70_RS00005"
                     /old_locus_tag="RRI70_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001287824.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     gene            481..747
                     /locus_tag="RRI70_RS00010"
                     /old_locus_tag="RRI70_00010"
     CDS             481..747
                     /locus_tag="RRI70_RS00010"
                     /old_locus_tag="RRI70_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024536.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     Typing          2019..2113
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.95e-42"
ORIGIN
        1 agcgcacatc cgcattttca ctgtctgctg atggtgcaac cttcttggtt taaagggaag
       61 aactacgtta agcacgaacg ttgggtagaa ctctggcgcg attgcttgcg ggtgaactat
      121 gagccgaata tcgatattcg ggcagtaaaa actaagacag gtgaggttgt ggccaacgtt
      181 gccgagcaac tgcaaagcgc ggttgctgaa acgctgaaat actccgttaa accggaagat
      241 atggcaaacg atcctgagtg gtttcttgag ctgacgcggc agcttcacaa gcgccgtttt
      301 atctcgaccg gtggggcgct aaaaaacgtc ctccagttgg atcgagaaac caatgaggat
      361 cttgtcattg ccgacgatgt aggggatggc actgatgacg ggaagcggac ggcgtttgtc
      421 tgggattcag gtaaacggcg ttacaaacgc gcccctgaga aggataaatc ggattaacgt
      481 atgaatatta atattgaata cctgaatgga aataagacta ttggtttatt ttttttaaga
      541 agtgaagcgg tgattcctga caggtttaaa aaccttattt tgcttattga tggattaagt
      601 tttggcacat ttggttttca tccgcacgaa ggttttgagg atgaattaat tttatatatt
      661 cagaaaacaa acgagagggt aaaaactctt tttgtgaaaa tagatttaaa cggtatcaag
      721 tttggatttt taagaacgca ttcttagttc tggaaaagag ccagcggcag gctgaggtga
      781 taggtacgag attgcatgca atctctagtg ctctgtctat cctgcattat cctcagcatt
      841 atcctcagca ttatcctcag cattatcctc agccttgcca actcgacacc aatgcaggat
      901 agacaatccg atgtcaaatg ttaacactct gcgagtggta cattttcccc ggattatcgt
      961 cctgagcctg ccgctggctc tctttctacc gcctcgcttt gctcgttgct caacgcctca
     1021 cagacacgga ttaaaatccg catccgttca ccgtttttta aagtccgtta aaagcatgat
     1081 gccatctccg agagttaatc tcgtcaaatg ctaaatcgtg ggggtcccct ttggggttcc
     1141 gatttagtga ttgacgacac caccgattaa aaaacttatg cggggtggat ggtttcacga
     1201 agtgaggcca tccacctgta agacagggtt ttgtttttat tccctgtttt ggtgatcggg
     1261 tgtgtggaaa aggttggggt aagccgttcg ggggtgcttg ttttgggggg ttaaaattgt
     1321 ggttattttt tgcgcaattc tcgcgcgtga tccttgtatt tatacttaag ggataaatgg
     1381 cggatatgaa atagtggttt agcccagtaa tgacgaggct ttgagtgggt tttgacaggt
     1441 caaagaaaat ggagcagaat tgaggcgttt ttaatcggcg ttggggagtg cgtcaacact
     1501 ccccaacatt tcgaatgtgt cacctcagcg gcaaactctg gtgacatgta ctggctcgca
     1561 atgcacaggt acgtgatgaa tataccacat caaatcacag cctgcccaga tcggagcagg
     1621 cttaatgtca gaagataaat tcctttcgga ctacagcccc cgtgatgcag tttgggatac
     1681 ccagcgcacg cttaccgatt ctgtcggggg tatctaccag actgctgctg aattcgagcg
     1741 ctatgcactc cgtatggcct cctgtagcgg tttgttacgt tttggttggt ctaccatcat
     1801 ggaaaccgga gaaacgcgcc tacggcttcg tagtgcgcaa ttttgccgtg tccgtcattg
     1861 ccctgtctgc cagtggagaa gaaccctcat gtggcaagcc cgtttttatc aggctctacc
     1921 gaaaatcgtt gtggattacc cgtcttcccg atggttgttt ctgacgttaa ctgtcaggaa
     1981 ctgcgagata ggtgaacttg gaacagtcct tacagcaatg aatgcggcgt ttaagcgaat
     2041 ggaaaagcga aaggagctat cacctgttca ggggtggatc agggctacgg aggtgacgcg
     2101 aggtaaggat ggc
//
