LOCUS       NZ_CP135217             7939 bp    DNA     circular CON 07-OCT-2023
DEFINITION  Escherichia coli strain Escherichia coli EC16019 plasmid pEC16019-2,
            complete sequence.
ACCESSION   NZ_CP135217
VERSION     NZ_CP135217.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37528250
            Assembly: GCF_032296485.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7939)
  AUTHORS   Peirano,G. and Pitout,J.
  TITLE     Genomic sequencing of MDR E. coli
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7939)
  AUTHORS   Peirano,G. and Pitout,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-SEP-2023) HMH-CDI, Hackensack-Meridian Health Center
            for Discovery and Innovation, 111 ideation way, Nutley, NJ 07110,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-SEP-2023
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Oxford Nanopore MiniION and Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/07/2023 06:56:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,982
            CDSs (total)                      :: 4,866
            Genes (coding)                    :: 4,645
            CDSs (with protein)               :: 4,645
            Genes (RNA)                       :: 116
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 221
            CDSs (without protein)            :: 221
            Pseudo Genes (ambiguous residues) :: 0 of 221
            Pseudo Genes (frameshifted)       :: 87 of 221
            Pseudo Genes (incomplete)         :: 148 of 221
            Pseudo Genes (internal stop)      :: 38 of 221
            Pseudo Genes (multiple problems)  :: 49 of 221
            Pseudo Genes (short protein)      :: 4 of 221
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP135217.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7939
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Escherichia coli EC16019"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC16019-2"
                     /geo_loc_name="Canada"
                     /collection_date="2016"
     gene            5..244
                     /locus_tag="RRI69_RS00015"
                     /old_locus_tag="RRI69_00015"
     CDS             5..244
                     /locus_tag="RRI69_RS00015"
                     /old_locus_tag="RRI69_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2164 family protein"
                     /protein_id="WP_000703021.1"
                     /translation="MKISDIEKNIYKGLAEFISQKIRDEHDICLGQFESEELLDEILKR
                     VIPVIYNVAIDDAIKSVRNVGDRLEEELDMRKVV"
                     /name="DUF2164 family protein"
     gene            241..603
                     /locus_tag="RRI69_RS00020"
                     /old_locus_tag="RRI69_00020"
     CDS             241..603
                     /locus_tag="RRI69_RS00020"
                     /old_locus_tag="RRI69_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="WP_000181070.1"
                     /translation="MTNFKSDLNLIRKEWYNAFYSGNTEMLDYLEADWFISTNGQKVIY
                     KKHQLRKIDMRPARILPSIERYEYDVVMREFKGIACVSGKAEIKQNDGNILMAFIENWI
                     KVNGGWKMQFISYEKI"
                     /name="DUF4440 domain-containing protein"
     gene            complement(628..1521)
                     /locus_tag="RRI69_RS00025"
                     /old_locus_tag="RRI69_00025"
     CDS             complement(628..1521)
                     /locus_tag="RRI69_RS00025"
                     /old_locus_tag="RRI69_00025"
                     /inference="COORDINATES: protein motif:HMM:NF025007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="WP_001061104.1"
                     /translation="MNNLIKKEIIEKFKKYNFQKYPFVFTDVNYKNLINWNDLNKLLEK
                     DILHYPRVRMANDNFPEIRGYKGFIRYTYSQTGDRTPHINRHQLYKCLRDGATLIVDRC
                     QSFFESVDESRLWLSKELECTCSANLYAAFTATPSFGLHFDNHDVIAVQIEGIKKWKVY
                     NPTYSYPLEDERSFDYLPPNTSPDYEFDITPGQAIYLPAGYWHNVTTQSKHSLHISFTV
                     IRPRRLELFKTLFDELKNNPYMREPIEHGDSLSDKEKIKTIITNAINSFDIQFAENMLK
                     AQSRTYRYKKIKLEHI"
                     /name="cupin domain-containing protein"
     gene            complement(1524..3116)
                     /locus_tag="RRI69_RS00030"
                     /old_locus_tag="RRI69_00030"
     CDS             complement(1524..3116)
                     /locus_tag="RRI69_RS00030"
                     /old_locus_tag="RRI69_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842142.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0042626 - ATPase-coupled transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_001009226.1"
                     /translation="MNFIEKYIITFNKCNLFFIILLSTISSFLFVLFGYLIKTVIDNKD
                     SIGEINSLLIFIACFLSIRFLMPAGYSISEYLTQKTNIELSVKLREQVIDNIQNSHQEH
                     FLKKNKGELNKVIESMLSSASSLFYTICSDVVPLLIQMIGIIITICISVNTLIAIEFII
                     IMAIYIIFVIKMTQRRFPMMKSVALSSKFASGRMFDMMHMYPMDKAFHTTDKSRKRVIQ
                     AVNTHSEKQRKVNNEFFLFGISSAFLSVIFSSLIILSAYWMFLHGRASRGSIIMLATFL
                     FQVFLPLNRIGYLFRQIKMARTEIDLYCAEINDIKKTNYTDKQYLHVKDNICNIEIYNK
                     SFNHKLILTKGVVTFITGENGSGKTTIAKILSGNITTEENTIKINGIQQEKANSPFVNV
                     LYVPQDLDLMPGTIEENILHYSGINNLNTIKKLLHRFKFDKPLDYEIKGYGHNLSGGQK
                     QKLGVSLTSGKNVDLIIFDEPTKGFDSLGIKIISDYIKEENKEKHIIVISHDEQLINNI
                     PKAQIIDITKHEV"
                     /name="ABC transporter ATP-binding protein"
     gene            complement(3155..3853)
                     /locus_tag="RRI69_RS00035"
                     /old_locus_tag="RRI69_00035"
     CDS             complement(3155..3853)
                     /locus_tag="RRI69_RS00035"
                     /old_locus_tag="RRI69_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000939065.1"
                     /translation="MLQHKMNSSSYAKVHNVSSLEDIMSYHNDDVLLKFRKEWNVTPEE
                     ADDIFNETKKFIWLASTCLTECYNIKVHEQLQIIDEMWHTFIQFTDAYTSFCEKYLGAY
                     LHHYPNTNDMLKNEIRHVNEHGITFQEYRFNEYKNQIEKIAFYLGHETVAKWYGDYAVR
                     YSIKNINTIRIPKESISSDSYIEKVKSITHLPAAEFVKIIMRKDVWNDNGSVCGCSGKG
                     CGAGCSCNSR"
                     /name="hypothetical protein"
     gene            complement(4401..4559)
                     /locus_tag="RRI69_RS00040"
                     /old_locus_tag="RRI69_00040"
     CDS             complement(4401..4559)
                     /locus_tag="RRI69_RS00040"
                     /old_locus_tag="RRI69_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071529495.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDKAPLAAATGNWTRWIEIL
                     RVLEVVA"
                     /name="replication initiation protein"
     gene            complement(5218..5409)
                     /locus_tag="RRI69_RS00045"
                     /old_locus_tag="RRI69_00045"
     CDS             complement(5218..5409)
                     /locus_tag="RRI69_RS00045"
                     /old_locus_tag="RRI69_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            5474..5797
                     /locus_tag="RRI69_RS00050"
                     /old_locus_tag="RRI69_00050"
     CDS             5474..5797
                     /locus_tag="RRI69_RS00050"
                     /old_locus_tag="RRI69_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955993.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVLAALMAIGEELRRLRLAVREQGAR
                     DDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            6006..>6437
                     /locus_tag="RRI69_RS00055"
                     /old_locus_tag="RRI69_00055"
                     /pseudo=""
     CDS             6006..>6437
                     /locus_tag="RRI69_RS00055"
                     /old_locus_tag="RRI69_00055"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            6475..6993
                     /locus_tag="RRI69_RS00060"
                     /old_locus_tag="RRI69_00060"
     CDS             6475..6993
                     /locus_tag="RRI69_RS00060"
                     /old_locus_tag="RRI69_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001673203.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETMLNDEFSKLEKSVSKAVTSNE
                     TKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVLFLFTVSG
                     GTLWYLGGMIQANLEEIRIQEETLQKLNAKTWGVEFVQDGRRKFLVIPQGKSATVIPYQ
                     GKDWVQLTE"
                     /name="MbeB family mobilization protein"
     gene            7000..7233
                     /locus_tag="RRI69_RS00065"
                     /old_locus_tag="RRI69_00065"
     CDS             7000..7233
                     /locus_tag="RRI69_RS00065"
                     /old_locus_tag="RRI69_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137439.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          5063..5142
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.78"
                     /coverage="92.0"
                     /evalue="8.94e-33"
     Typing          complement(4488..5030)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.679"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          5442..5791
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.872"
                     /coverage="99.0"
                     /evalue="9.42e-67"
     Typing          5788..7337
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.787"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aagaatgaaa atttctgata ttgaaaaaaa catatataaa ggactagctg aatttatttc
       61 acaaaaaatc agagatgaac atgatatatg ccttggacag tttgaatcag aggaattact
      121 tgatgagatt cttaaaagag taatcccagt catatataat gtggctattg atgatgctat
      181 aaaatcagta aggaatgttg gggatagatt agaggaggaa ttagatatga ggaaggtagt
      241 gtgactaatt ttaaatcaga cttaaatcta ataagaaaag aatggtataa tgcattttat
      301 tcaggtaata ctgaaatgct agattacttg gaagctgatt ggtttatttc aacaaatggt
      361 caaaaagtta tctataaaaa acatcagtta cgaaagattg atatgcgtcc agcgaggatt
      421 ttacctagca tagaaagata tgaatatgat gtcgtaatgc gtgagtttaa aggcattgct
      481 tgtgtttcag ggaaagctga aataaagcaa aatgatggaa atatattaat ggcttttatt
      541 gagaattgga taaaggttaa tggtggatgg aaaatgcagt ttatatcgta tgaaaaaatc
      601 taaggggaag cccccttaga ttaatggtta tatatgttca agttttattt ttttatagcg
      661 ataagttctg ctttgagctt taagcatgtt ttctgcaaac tgaatatcaa aactatttat
      721 agcattagtt attatagttt ttattttttc tttatcagat aatgaatcac catgctctat
      781 aggttcccgc atatatggat tgttttttaa ttcatcaaat aatgttttaa atagttctaa
      841 tcgacgaggt cttataactg taaaagatat atgaagagag tgtttgctct gagtcgtaac
      901 attgtgccag tatccagcag gaagatatat ggcctgacct ggggttatat caaactcata
      961 atctggggag gtattaggtg gtagataatc gaagcttctt tcatcttcga gagggtatga
     1021 ataggtaggg ttgtaaactt tccatttttt tattccctca atttgaacag ctattacatc
     1081 atgattgtca aaatgaagcc caaagcttgg tgttgctgta aatgcagcat atagattagc
     1141 actacatgta cattctaact ctttagataa ccatagtctg ctttcatcta cagattcaaa
     1201 gaatgactgg catcgatcta ctataagggt tgcgccatca cgtaagcatt tatatagctg
     1261 atggcgattt atatgtggtg ttctatcgcc tgtttggcta tatgtgtatc ttataaatcc
     1321 tttatacccc ctaatttcgg gaaagttgtc atttgccatt ctaactctag gatagtgcaa
     1381 tatatctttt tcaagcaatt tattaagatc attccagttg attaagtttt tataattaac
     1441 atccgtgaat acaaacggat atttctggaa attatatttc ttaaattttt ctatgatttc
     1501 cttttttata agattattca tttttaaacc tcatgttttg tgatatctat aatctgtgct
     1561 ttaggtatat tatttataag ttgttcatca tgagatataa caatgatatg cttttccttg
     1621 ttttcctctt ttatataatc actgattatt tttataccaa gtgaatcaaa cccttttgtc
     1681 ggttcatcga aaataatcaa gtctacgttt tttccagatg taagagatac tcctaatttt
     1741 tgtttttgtc caccagataa attatgtccg taacctttga tctcataatc tagtggtttg
     1801 tcgaacttaa atctgtgtag cagcttttta attgtattta aattattaat gccggaataa
     1861 tgtaatatat tttcctctat agttccaggc atgagatcta gatcttgagg aacgtataag
     1921 acattaacaa aaggagaatt tgctttttct tgttgtatcc cattgatttt tattgtgttt
     1981 tcttctgttg ttatattgcc agagagaatt tttgcaatag ttgtttttcc cgaaccattc
     2041 tcaccggtta taaaggtaac aacgcctttt gttaatatta atttatggtt gaatgatttg
     2101 ttgtatattt caatattgca aatattgtct tttacatgaa gatattgctt gtctgtgtaa
     2161 tttgtttttt ttatgtcgtt tatttcggca cagtataaat caatttctgt tcttgccatt
     2221 tttatttgtc taaatagata accaatgcga tttaatggga gaaaaacttg gaataaaaaa
     2281 gtggcaagca taataatact accacggctt gctctaccat gaagaaacat ccagtatgct
     2341 gataggataa tcagactact aaaaatgact gaaagaaaag cggagctgat accaaacagg
     2401 aaaaattcat tatttacttt tctttgtttt tctgaatgtg tatttacagc ttgaattact
     2461 cgtttacgac ttttatccgt ggtatggaaa gctttatcca taggatacat gtgcatcata
     2521 tcaaacattc ttccagatgc gaatttggag ctaagtgcaa ctgatttcat cattggaaat
     2581 ctacgttgtg tcatttttat gacaaatatt atatagattg ccattattat tataaactca
     2641 atggctatta atgtattaac acttatacaa attgttatga ttattccaat catttgtatt
     2701 aataatggta ctacatcaga acatattgta taaaataatg atgatgcaga agaaagcatg
     2761 ctttctataa ccttatttag ttctccttta tttttcttta aaaaatgctc ttggtgagaa
     2821 ttttgtatat tatcaataac ttgctctcta agctttacag ataattctat atttgttttt
     2881 tgagtcaagt attccgatat gctatatcca gcaggcataa ggaatctaat tgataagaaa
     2941 catgctataa atattaataa tgagtttatt tctcctattg aatctttatt gtcaataact
     3001 gtttttatta aatatccgaa tagaacgaaa agaaaactac ttatagttga taataatata
     3061 ataaaaaaca aattacattt attaaatgtg atgatgtact tttcaataaa attcaattta
     3121 taatccttaa agcccgtata ttacgggctt tatgttatct agaattacat gagcacccag
     3181 cgccacatcc tttaccgctg caaccacaaa cagaaccatt atcattccaa acatcttttc
     3241 gcattattat ttttacaaat tctgcggctg gaaggtgagt gatactcttt actttttcga
     3301 tgtaagaatc actggatatg gattcttttg gaattcttat agtattaatg ttctttatgc
     3361 tgtatcttac agcataatca ccataccact ttgcgacagt ttcatgacct aggtaaaaag
     3421 cgattttttc aatttgattt ttatattcgt taaaacgata ttcttggaat gttataccat
     3481 gctcattaac atgccttatc tcatttttta gcatgtcatt tgtatttgga taatggtgga
     3541 ggtaagcacc aagatatttt tcacaaaagc tggtataagc atctgtaaat tgaataaacg
     3601 tatgccacat ttcatctata atttgtaatt gctcgtgaac ttttatattg tagcattccg
     3661 ttaggcatgt tgaggctagc cagatgaatt tctttgtttc attaaagata tcatcagctt
     3721 cttctggtgt tacattccat tctttacgaa attttagaag aacatcatca ttgtgataac
     3781 tcatgatatc ttctaatgag ctaacattat gaacttttgc ataagaactg ctgttcatct
     3841 tatgttgtag cataacaccc ccaattatat atttgaatgt agatgagaat agttatcatt
     3901 tgttatggta ttgttaaatt tgtgcttgtg tcaatatgcc taattctata aatgagatag
     3961 taatcaagtt ttttaatggt tataggatcc attagtaata caaggttttt gcagccagtt
     4021 tttacgggta ggggctgcaa ggaaaacaaa attttcccac tctaagagaa gagaattgag
     4081 ttggttgaat gagtggatgc atctagggcc gctaattcac cgagtgtaaa ggctgtacga
     4141 aatgtgttgc agtccgataa tagccccctc cattgacgtt ttacacatgt gttgtaggtt
     4201 ccaggagtgg gcggatctca aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     4261 cgtaatctgc tgccagtaaa cgaaaaaacc gcctggggag gcggtttagt cgaaggttaa
     4321 gtcagttggg gactgcttaa ccgggtaact ggcttggtgg agcacagata ccaaatactg
     4381 tccttctagt gtagccgcag ttaggccacc acttcaagaa ctcttaatat ctcaatccac
     4441 cttgtccagt taccagtggc tgctgccagt ggcgctttgt cgtgtcttac cgggttggac
     4501 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     4561 cagcccagct tggagcgaac gacctacacc gagccgagat acctacagcg tgagctatga
     4621 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     4681 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     4741 gtcgggtttc gccacctctg acttgagcgt cgatttttat gatgctcgtc aggggggcgg
     4801 agcctatgga aaaacgccaa cggcgcagcc ttttcctggt tctcgttttt tgctcacatg
     4861 tttcttttgg cgttatcccc tgattctgtg gataaccgca tctccgcttt tgagtgagca
     4921 gacaccgctc gccgcagccg aacgaccgag tgtagcgagt cagtgagcga ggaagcggaa
     4981 gagcgccgga acgtgcattt tctccttacg catctgtgcg gcatttcaca tcggacatgg
     5041 tgcgctttcc atacaattcg tactgatgcc gcatagttaa gccagtatac actccgctat
     5101 cgctacgtga ctggttcagg gcttcgcccc gaaaccccct gacgcgccct gaggggcttg
     5161 tctgctcccg gcatccgctc acagacaagc tgttaccgtc tccgggagct gtatgtgtca
     5221 gaggttttca ccgtcatccc cgaagcgtgc gaggcagctg cggtaaagct cgtcggcgtg
     5281 gtcgtgaagt gattcacaaa tatcggcctg ttcatctgcg tccagttcgt tgagtttctc
     5341 cagcagcgtt aatgtctggc ttctgataaa acgggccatg ttaagggcgg ttttttcctg
     5401 tttagtcact gatgcctccg tgtaaggggg atttctgttt tatggggtaa tgataccgat
     5461 gaaacgcgag aggatgctca ccatacgggt tactgatgat gaacatgccc ggttactgga
     5521 acgctgtgag ggtaaacagc tggcggtatg gatgcggcgg gtctgcctgg gtgagccggt
     5581 tgcacgctcc gggaagctgc cgacactggc accgccgtta ctgcgtcagc tggccgccat
     5641 cgggaataac ctgaatcaga cagcgaggaa ggtgaacagc gggcagtggt cttccggtga
     5701 tcgggttcag gtgttggctg cgctgatggc catcggggag gagctgcgtc ggctgcgtct
     5761 ggccgtcagg gaacagggcg cgcgggatga ttgttaaatt tcatgccagg ggaaaaggcg
     5821 gtggcagtgg tccggttgat tacctgctgg gaagggagcg aaaccgggaa ggggcaacgg
     5881 tgcttcaggg taatccggaa gaagtccggg aactcatcga tgccacgcca tttgcgaaga
     5941 aatacacgtc aggtgttctg tcgttcgcgg agaaggagct gccgccggga ggacgtgaaa
     6001 aggtgatggc gagctttgag cgtgtactga tgcccggtct cgaaaagaat cagtacagca
     6061 tcctgtgggt ggagcaccag gacaagggac ggcttgagct gaattttgtc attccgaaca
     6121 tggagctaca gaccggaaaa cgcctccagc cgtactacga ccgggcagac aggccgagaa
     6181 ttgatgcctg gcagacgctg gtgaatcacc attacgggct gcatgacccc aacgccccgg
     6241 agaaccgccg gatactgact ctccctgata acctgccgga aacgaaacag gcgcttgcag
     6301 agagcgtcac acgcgggata gacgctcttt atcatgtcgg ggagataaaa ggtcgtcagg
     6361 atgtgattca ggcgctcaca gaggcagggc ttgaagtggt cagggtgatg cgaagcagta
     6421 tcagcattgc agacccgaac ggtgggaaga atatcaggct gaaaggagca ttttatgagc
     6481 aatcttttac agacgggcgc ggagttcgag aaaaagctga aagagagagc cgaatctaca
     6541 gagacaatgc tgaacgacga gttcagcaag ctcgaaaaat ctgtaagcaa ggctgtgaca
     6601 tcaaacgaga cgaaaatcaa agacgctata gccctgttca cagcttcgac agaggaatca
     6661 ctgaaaaaac accgggaagg ggtgaaagag gcgatgatgc agcacaggaa ggacgtgtta
     6721 aagctggcag ggaatacggg catgatgtta ctggggatgg tccttttcct gtttaccgtg
     6781 agtggcggga cgctctggta tcttggcggg atgatacagg cgaacctgga agaaatcagg
     6841 atacaggaag aaacattgca gaaactgaac gcgaagacat ggggcgtgga gtttgtgcag
     6901 gacgggagaa ggaaatttct ggttatccct caggggaaat cggcaacggt gattccctat
     6961 caggggaagg actgggtaca actgacggag tgacacagag tgacggagct ggaaagacat
     7021 ttgctgaacg ccttagagca actgcaacag gactatatgc agcggctgaa cgaatgggag
     7081 agcgccttcg tggaattgca gaaaatgttt tcgcttacgc aacgggacaa cgcgatgctg
     7141 aacgagcggg tcatgcagtt gagtcagcag gtgcagcact tgagcgagca gacagaacgc
     7201 ttgagccagt tatacagcga gaacaggaga taagggatga acggctgata caggaacgcg
     7261 aacatgagtt atccctggag cgtgagcgcc agctggaaat acaggaacgg acacaggatg
     7321 gcccttcgct gggatggtaa tcagagacag gctaaaatgt tgtcagcgcg tttccggtgc
     7381 attttttggg ataatggttg gttcgggggc accgggaacg cgcttcagtc agaccttgct
     7441 gaaaacacag gaacgcgtta aaacgcatac gggcgcgatt ctggtgtgtc ttttgttgct
     7501 gtgttttgcc tctattgcca gatcgcgctc attcccctga acttctgttt tcccgctccg
     7561 gtccccgtct tcttcgcttc cgaaagcatg aaaaaaatcc gtttcagctg tctctggctg
     7621 gcaattcctg cgtgattcac atggctgcat agctatgcat gtggttaaaa tttaccgggt
     7681 gcgatcgcgg cagtttttcg ggtggtttgt tgccattttt acctgtctgc tgccgtgatc
     7741 gcgccgaacg cgtttcagcg gtgcgtacaa taagggatta tggtaaatta tcttgttcct
     7801 tagagaatga tttgaggtgg tatcctgcac ttatttaaat atgcttgctt gggaaattcc
     7861 aggttgcagt atggttgagt tgtaattttc ctatgatagg aaaaatgtta tattattaat
     7921 ttatttgtga ggcttcata
//
