LOCUS       NZ_CP135216             2113 bp    DNA     circular CON 07-OCT-2023
DEFINITION  Escherichia coli strain Escherichia coli EC16019 plasmid pEC16019-3,
            complete sequence.
ACCESSION   NZ_CP135216
VERSION     NZ_CP135216.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37528250
            Assembly: GCF_032296485.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Peirano,G. and Pitout,J.
  TITLE     Genomic sequencing of MDR E. coli
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Peirano,G. and Pitout,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-SEP-2023) HMH-CDI, Hackensack-Meridian Health Center
            for Discovery and Innovation, 111 ideation way, Nutley, NJ 07110,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-SEP-2023
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Oxford Nanopore MiniION and Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/07/2023 06:56:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,982
            CDSs (total)                      :: 4,866
            Genes (coding)                    :: 4,645
            CDSs (with protein)               :: 4,645
            Genes (RNA)                       :: 116
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 221
            CDSs (without protein)            :: 221
            Pseudo Genes (ambiguous residues) :: 0 of 221
            Pseudo Genes (frameshifted)       :: 87 of 221
            Pseudo Genes (incomplete)         :: 148 of 221
            Pseudo Genes (internal stop)      :: 38 of 221
            Pseudo Genes (multiple problems)  :: 49 of 221
            Pseudo Genes (short protein)      :: 4 of 221
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP135216.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Escherichia coli EC16019"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC16019-3"
                     /geo_loc_name="Canada"
                     /collection_date="2016"
     gene            586..1551
                     /locus_tag="RRI69_RS00005"
                     /old_locus_tag="RRI69_00005"
     CDS             586..1551
                     /locus_tag="RRI69_RS00005"
                     /old_locus_tag="RRI69_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001287824.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     gene            1555..1821
                     /locus_tag="RRI69_RS00010"
                     /old_locus_tag="RRI69_00010"
     CDS             1555..1821
                     /locus_tag="RRI69_RS00010"
                     /old_locus_tag="RRI69_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024536.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     Typing          980..1211
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 gcatccgttc accgtttttt aaagtccgtt aaaagcatga tgccatctcc gagagttaat
       61 ctcgtcaaat gctaaatcgt gggggtcccc tttggggttc cgatttagtg attgacgaca
      121 ccaccgatta aaaaacttat gcggggtgga tggtttcacg aagtgaggcc atccacctgt
      181 aagacagggt tttgttttta ttccctgttt tggtgatcgg gtgtgtggaa aaggttgggg
      241 taagccgttc gggggtgctt gttttggggg gttaaaattg tggttatttt ttgcgcaatt
      301 ctcgcgcgtg atccttgtat ttatacttaa gggataaatg gcggatatga aatagtggtt
      361 tagcccagta atgacgaggc tttgagtggg ttttgacagg tcaaagaaaa tggagcagaa
      421 ttgaggcgtt tttaatcggc gttggggagt gcgtcaacac tccccaacat ttcgaatgtg
      481 tcacctcagc ggcaaactct ggtgacatgt actggctcgc aatgcacagg tacgtgatga
      541 atataccaca tcaaatcaca gcctgcccag atcggagcag gcttaatgtc agaagataaa
      601 ttcctttcgg actacagccc ccgtgatgca gtttgggata cccagcgcac gcttaccgat
      661 tctgtcgggg gtatctacca gactgctgct gaattcgagc gctatgcact ccgtatggcc
      721 tcctgtagcg gtttgttacg ttttggttgg tctaccatca tggaaaccgg agaaacgcgc
      781 ctacggcttc gtagtgcgca attttgccgt gtccgtcatt gccctgtctg ccagtggaga
      841 agaaccctca tgtggcaagc ccgtttttat caggctctac cgaaaatcgt tgtggattac
      901 ccgtcttccc gatggttgtt tctgacgtta actgtcagga actgcgagat aggtgaactt
      961 ggaacagtcc ttacagcaat gaatgcggcg tttaagcgaa tggaaaagcg aaaggagcta
     1021 tcacctgttc aggggtggat cagggctacg gaggtgacgc gaggtaagga tggcagcgca
     1081 catccgcatt ttcactgtct gctgatggtg caaccttctt ggtttaaagg gaagaactac
     1141 gttaagcacg aacgttgggt agaactctgg cgcgattgct tgcgggtgaa ctatgagccg
     1201 aatatcgata ttcgggcagt aaaaactaag acaggtgagg ttgtggccaa cgttgccgag
     1261 caactgcaaa gcgcggttgc tgaaacgctg aaatactccg ttaaaccgga agatatggca
     1321 aacgatcctg agtggtttct tgagctgacg cggcagcttc acaagcgccg ttttatctcg
     1381 accggtgggg cgctaaaaaa cgtcctccag ttggatcgag aaaccaatga ggatcttgtc
     1441 attgccgacg atgtagggga tggcactgat gacgggaagc ggacggcgtt tgtctgggat
     1501 tcaggtaaac ggcgttacaa acgcgcccct gagaaggata aatcggatta acgtatgaat
     1561 attaatattg aatacctgaa tggaaataag actattggtt tatttttttt aagaagtgaa
     1621 gcggtgattc ctgacaggtt taaaaacctt attttgctta ttgatggatt aagttttggc
     1681 acatttggtt ttcatccgca cgaaggtttt gaggatgaat taattttata tattcagaaa
     1741 acaaacgaga gggtaaaaac tctttttgtg aaaatagatt taaacggtat caagtttgga
     1801 tttttaagaa cgcattctta gttctggaaa agagccagcg gcaggctgag gtgataggta
     1861 cgagattgca tgcaatctct agtgctctgt ctatcctgca ttatcctcag cattatcctc
     1921 agcattatcc tcagcattat cctcagcctt gccaactcga caccaatgca ggatagacaa
     1981 tccgatgtca aatgttaaca ctctgcgagt ggtacatttt ccccggatta tcgtcctgag
     2041 cctgccgctg gctctctttc taccgcctcg ctttgctcgt tgctcaacgc ctcacagaca
     2101 cggattaaaa tcc
//
