LOCUS       NZ_CP135208             4072 bp    DNA     circular CON 07-OCT-2023
DEFINITION  Escherichia coli strain Escherichia coli AZ1114 plasmid pAZ1114-4,
            complete sequence.
ACCESSION   NZ_CP135208
VERSION     NZ_CP135208.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37528247
            Assembly: GCF_032292885.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   Peirano,G. and Pitout,J.
  TITLE     Genomic sequencing of MDR E. coli
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4072)
  AUTHORS   Peirano,G. and Pitout,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-SEP-2023) HMH-CDI, Hackensack-Meridian Health Center
            for Discovery and Innovation, 111 ideation way, Nutley, NJ 07110,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-SEP-2023
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Oxford Nanopore MiniION and Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/07/2023 07:04:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,903
            CDSs (total)                      :: 4,785
            Genes (coding)                    :: 4,511
            CDSs (with protein)               :: 4,511
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 95 of 274
            Pseudo Genes (incomplete)         :: 177 of 274
            Pseudo Genes (internal stop)      :: 46 of 274
            Pseudo Genes (multiple problems)  :: 41 of 274
            Pseudo Genes (short protein)      :: 4 of 274
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP135208.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Escherichia coli AZ1114"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pAZ1114-4"
                     /geo_loc_name="Mexico"
                     /collection_date="2015"
     gene            complement(join(3896..4072,1..1971))
                     /gene="mobQ"
                     /locus_tag="RRI72_RS24475"
                     /old_locus_tag="RRI72_24475"
     CDS             complement(join(3896..4072,1..1971))
                     /gene="mobQ"
                     /locus_tag="RRI72_RS24475"
                     /old_locus_tag="RRI72_24475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_001029354.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPHELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAIGPAGFSGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNGGLTEPARRVADLLNIGLPDNATSYTLRDIIEALPKDSN
                     SAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQMAFEKQWQQWGESGLAALRAQL
                     LKREEELRRKEQEDTERSRAEQLRKNDNAVMDSGVLRAVVTGYGEAPMPGNGEMTCYLL
                     LHNRNGEYTLWGNELEKYRTRIFESVDLMRDRSGYICDRSEIGQRPPLQRVYSSATFEQ
                     LLTQVCQTWPQHTRNLRQPKTWPESFCLGEDRQPAMPSPAARKVDFMQGRLPPTLMPVM
                     SSVDRETRQLQLLLVMGVDDSLGGVIRLNGTLYPAFAVPSADNRHLVISALTDKGLRYA
                     GYGVAVNHDADSHISPAPELMQFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRTWEEW
                     QREEDARTHTTERRHDRGWSQ"
                     /name="mobQ"
     gene            2150..2404
                     /gene="mobC"
                     /locus_tag="RRI72_RS24480"
                     /old_locus_tag="RRI72_24480"
     CDS             2150..2404
                     /gene="mobC"
                     /locus_tag="RRI72_RS24480"
                     /old_locus_tag="RRI72_24480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091746.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPEQ"
                     /name="mobC"
     gene            2937..3638
                     /locus_tag="RRI72_RS24485"
                     /old_locus_tag="RRI72_24485"
     CDS             2937..3638
                     /locus_tag="RRI72_RS24485"
                     /old_locus_tag="RRI72_24485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281395.1"
                     /translation="MSDLIAYKSNALIEASYKLTLQEQRFLLVCISRLKSGEDSPSPDE
                     QKTMTITAAEFHDAFPDMGRQHAEARLKEAIDRLWDRSVIIKNEEKREEFRWVQYRAQY
                     FKGEAKVDITFSDAIMPYLTQLKGQFTRVVVKNVSSLSSTYSIRIYELLQQFRSTGDRT
                     IAIDDFRTMLAVEDKYQQFKDLNKMIIKPAIAELNEKSDLVVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     Typing          complement(1614..1972)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1973..2149
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          2938..3638
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.858"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2..2058)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.087"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ctgcatcagt tctggtgcag gtgatatgtg gctgtctgca tcgtgattta cggcaacacc
       61 ataccctgca taccgtaacc ccttatcggt cagcgcactg atgaccagat gcctgttatc
      121 ggctgaggga acggcaaacg cgggatacag cgtaccatta agccggataa caccgcccag
      181 agagtcatcc acgcccatga ccagcaaaag ctgaagctgg cgggtttccc ggtcgacaga
      241 actcatgacc ggcatcagcg tcggtggcag acgtccctgc ataaaatcca ccttccttgc
      301 cgctggtgat ggcatggctg gctggcggtc ttctccaaga cagaaagact ccggccatgt
      361 cttcggctgg cggagattgc gggtatgttg cggccatgtc tggcaaacct gcgtcagcag
      421 ttgctcaaag gttgcagagg aataaacacg ttgcagtggc ggacgttggc cgatttcgct
      481 tcggtcacag atataaccac tcctgtcacg cattaaatca acactctcaa agattctggt
      541 gcggtatttc tccagctcgt tcccccagag cgtatattct ccgttccggt tgtgaagcag
      601 aaggtaacag gtcatttccc cgttaccagg catgggagcc tcgccatacc ccgtaacgac
      661 tgctctcagc acccctgaat ccataacggc attgtcattt ttcctgagct gctccgcgcg
      721 actacgttca gtatcttcct gttccttccg gcgcaattcc tcttcacgtt taagcaactg
      781 ggctctgaga gctgcaaggc cggattcccc ccactgctgc cactgttttt caaatgccat
      841 ctgactcgcg ggaatgtcct gtttctccac cttccggtaa tgctgtcgta atttttccat
      901 ttgctggctc agagactgga tttttccctg ctcactacgg tattccggtg ccgcccattg
      961 catcagggga atacgggaaa aaccggacga aaacggcgat gattttttca ggctggcagc
     1021 atggctcatc gcttcctttc gttcggtgtt cagctcttcc cagcgtctgc gcgtcgcctg
     1081 catatctgcc atcatcgaca tggcattact ggtgagtttc catgccgagt tactgtcctt
     1141 cggcaacgct tcgataatgt cacgtagtgt gtacgaagtt gcgttgtcag gaagcccgat
     1201 attcagaagg tcagccacac ggcgggcagg ctctgtcagc cctccgttcc tgagttcggt
     1261 gcgggaaatc tgtatttcca ggttaagcct gttaatcaga cgattctgtt cgccgcgttc
     1321 tgtttcgata ccgcgtgttt ccatcgcaca ggcagacttt ccgaggtgaa taccaggcgt
     1381 tttctcaagc ccctgtcgct cgtatgaacg atggtctatc tcttcctgat aaccggcgtt
     1441 cgccagtgcc ctgtttgcat ggtcagccca tgatgcccgc cacgtctcgg catgtgtccg
     1501 gtcgttccag tccctgacct ttccgctgaa cccagcaggt ccgatagccc tcgtggtcag
     1561 catgatgtgc gcatggggat ttgttttatc catgtgatgg aacgccacat cagcaatcat
     1621 gccctgactg acaaagtttt ctctgacaaa ggcgagaacg gtttcccgtg cggcatcctg
     1681 cggcagttcg tgggggatgg cgagttcaat ttcccgcgca agttgtgagt tcttccggcg
     1741 ttcggccttc tcaacggcat tccagagttc ggcacggttc gtacaccagg caggggcggt
     1801 ggcgggggtc agaatcacag cctcagccac accgcttttg cgggtgtagt cgtgagtcag
     1861 tccggtacgc tcatcaagca tcagtgaacc ggaacgatag gcagcggaag ctacggcgct
     1921 gtagccgctg cttctggaga taattttcat gctgagatga taaatcgcca tttctgccgt
     1981 aatgcctttt tgtagttttc tttcagcccc gcagggcgca cacaatcgaa acggagtgga
     2041 gattgtataa gtgcgcactt cgtgatttgc cgtgcattat gccagctcag tttttaaact
     2101 accagtgacg cgctaaaatg cgatttttca tcgcgcacgg agttacatca tgagcagaac
     2161 gctcgaacag aagattgctg atgctgaggc gagattacag cgcctgaagg cgaagagccg
     2221 gagcctggac acagcgcaga aggttgttgt gggtgctgca ttgctggcaa aggtcagaaa
     2281 gccggaggag gtgcagttgc gtgcctggtt actccagttc ctgaaggcag aggtgacacg
     2341 acaggctgat gtgacgcgca tactgccgct gattaacgag ctggaggcgc tgccagaaca
     2401 gtgattgtat cgtccgctcg ccgcagcccc agcgaccgag cgttagcgag cgagtgggcg
     2461 aggaagcgga agtgaatcag tcgccaggtt gagcacatac gcacggtttt tcggcaggtg
     2521 atatgcgctg aacccgcatg aatactgggt ttccacaggg ttggaggggg tgtattcgaa
     2581 acttgcaatt ctgctttatt tcaacgcatt gtttttattg gttttttgtt ttcagcaccc
     2641 caagttatcc acaggctcgc cgttaagttt ttaagatctt atttttagat ctttttttta
     2701 aaaccttttt aggtcttgta acaccatgat tttaaagatg ttctggcaag ataagtggag
     2761 cttttggggg tgaaaggtgg agcttttggg ggtgaaaggt ggagcttttg ggggtgaaag
     2821 gtggagcttt tgggggtgta atccacagaa aaagggggtt gcaaaaaaaa aacttccacc
     2881 catatgataa gtggcgtaca gtaaaaaaac gccaccaata gcaacgagca agcgaaatgt
     2941 cagatctgat agcttacaaa tcaaacgccc ttatcgaggc aagctacaag ctcactttgc
     3001 aggaacagcg cttcctgctg gtctgtatca gccgcctgaa atccggagaa gattcccctt
     3061 cgccggatga acaaaaaacc atgaccatca cggctgcaga gtttcatgat gcctttcctg
     3121 acatgggacg gcagcacgct gaagctaggc tcaaggaagc gatagatcgc ctctgggaca
     3181 ggtcagtaat catcaagaac gaggaaaaac gagaagagtt ccgctgggtg cagtaccggg
     3241 cgcagtattt caaaggtgag gcgaaagtag atatcacctt ctccgatgcc atcatgccgt
     3301 atctgacaca gctcaagggg cagtttacgc gggtagtcgt gaagaacgtc tccagccttt
     3361 ccagcaccta cagcatccgc atttacgagc tgctccagca gttccgcagc accggtgaca
     3421 gaacgatagc tatcgatgat ttccgaacca tgcttgccgt tgaagataaa tatcaacaat
     3481 tcaaagactt gaataagatg ataatcaagc cagccatcgc cgaactgaac gaaaaaagcg
     3541 atctggttgt aactgttgag acgatcaaaa aggggcgcac ggttgtagcc ctgcattttc
     3601 gcttcaagga agacaaacag atcaagatga cgatctaaaa tcgattttaa ggcacctaaa
     3661 acgctccctg ctgcgttttt tttgtgttag tagtgcgaag gtactattga agtgaaaaat
     3721 tgcgtcaaaa acgctctgat gcagcgaaaa aagacccccc aatttttggg ggcggaaaaa
     3781 caaaactatg cttgcgttat gtgagtgtgc ttcttgcatt catccctgtc gatgaacatc
     3841 gaagaacatc gacgaacata acacgatgtt aaacaaaaca aaatattgtt cacattcact
     3901 gtgaccaacc gcgatcatgt ctcctctcgg tggtgtgtgt tcgtgcatct tcctcgcgct
     3961 gccattcctc ccaggtacga ttaaaaccca gcgagcggaa cagatgatcc ggctgttttt
     4021 ctggtgtgtt gacggcagca aaaagcggtg cctcacgggt tttcagatga aa
//
