LOCUS       NZ_CP135171             2987 bp    DNA     circular CON 04-OCT-2023
DEFINITION  Klebsiella pneumoniae strain L3995hy plasmid pL3995-7, complete
            sequence.
ACCESSION   NZ_CP135171
VERSION     NZ_CP135171.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37449614
            Assembly: GCF_032248875.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Liu,X.
  TITLE     Existance of tet(X4) in Klebsiella pneumoniae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2987)
  AUTHORS   Liu,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-SEP-2023) Jinan Provincial Laboratory of
            Microecological Biomedicine, Shandong First Medical University,
            No.6699 Qingdao Road, Jinan 250117, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. v. 4.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/04/2023 13:30:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,450
            CDSs (total)                      :: 5,326
            Genes (coding)                    :: 5,197
            CDSs (with protein)               :: 5,197
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 52 of 129
            Pseudo Genes (incomplete)         :: 76 of 129
            Pseudo Genes (internal stop)      :: 20 of 129
            Pseudo Genes (multiple problems)  :: 15 of 129
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP135171.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="L3995hy"
                     /isolation_source="human stool"
                     /db_xref="taxon:573"
                     /plasmid="pL3995-7"
                     /geo_loc_name="China:Hangzhou"
                     /collection_date="2021-07"
     gene            115..306
                     /locus_tag="RND80_RS27240"
                     /old_locus_tag="RND80_27240"
     CDS             115..306
                     /locus_tag="RND80_RS27240"
                     /old_locus_tag="RND80_27240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041614.1"
                     /translation="MNKQQKTAVNMAKFIQDQSLLLLDRLNELDLDHEADFCEKLHEDA
                     ERLYRSLSARLNEQQDGA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1616..1864
                     /locus_tag="RND80_RS27245"
                     /old_locus_tag="RND80_27245"
     CDS             1616..1864
                     /locus_tag="RND80_RS27245"
                     /old_locus_tag="RND80_27245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017096688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_029793011.1"
                     /translation="MKNVRMQFDLPEDRLDELDSLMKKCGISTKKELFNYALTMLEWAV
                     DESESGHEIAAIDRDSKQFYALRMPILKRVNRTSTAN"
                     /name="hypothetical protein"
     gene            1937..2308
                     /locus_tag="RND80_RS27250"
                     /old_locus_tag="RND80_27250"
     CDS             1937..2308
                     /locus_tag="RND80_RS27250"
                     /old_locus_tag="RND80_27250"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_024242345.1"
                     /translation="MSDSLDGFTEGDTSKEKQAVKQPKKFFSDVDDSADRRHLRWTKSG
                     VTLAMTILVAVMNWMVISMLYHVVDEELSLIRSGFMQADARTVTTSVYLALIAGTVAEV
                     SALFLIIVKSVFKNNNNDI"
                     /name="hypothetical protein"
     Typing          complement(17..240)
                     /name="rep_cluster_2327"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.667"
                     /coverage="84.0"
                     /evalue="1.71e-93"
     Typing          complement(2378..2803)
                     /name="rep_cluster_2374"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.766"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ctgcttatct tccctccgct cgccgcagac tcatgaccga acggagtgag cgaatgagcg
       61 aggaagcgga aaaccatcag aacgtacagt aagcactttc gcgccggggc tttcatgaac
      121 aaacaacaaa agactgccgt taacatggct aaattcatcc aggatcagtc cctgcttctt
      181 ctggacaggc ttaatgagct ggatctggat catgaggctg atttctgcga gaaactccat
      241 gaggatgcgg aacgccttta ccgaagcctg tcagcgcgcc tgaatgagca gcaggacggt
      301 gcgtaatggc tccgttctgg gtattaccgg agtaataccg gcatcgcctt aattttcatt
      361 gtcttagcgc ttaacattat taccccggta atacccagaa tccctgtatt ttttcttttt
      421 tatttatttt tcatgcagtt gcgatgaaag tcgcggctgc gttaggtgta tgaccatttt
      481 gaggttgaaa aataatcaac catggcgggt gatttacgca ggttaagggg agatcgtgac
      541 aaaaaaagcg ttcctcactg tatcgttcat gcctgcatga acgggttatt cttcctgact
      601 acagaattat atcgctcctt cagttcatac atcactttta caacactcct tcagtctgta
      661 catcactttt acaacacttt tgcatcactt ttacatcatt tgtacatcac ttttacatca
      721 ttccttcagc tcatacgtca cttttacgtc agttccatcc tctgtgccat ttgtacatcc
      781 gacccccttc ctgaacgtgc tgaaaacggt ttttccggct gtgccggcaa cgcctcacgg
      841 ccacgcaata aattgcgtcc ccgttcaccg ttttcggata agaagaacat cggaaacagg
      901 acttaccccg gctgaatggc gtgaatttcc gcgctatgca cttgcgcgca tactcatgca
      961 tgccgtaaaa acagagcctg cgcgtttctg gcgggttttc gggtggtttg ttgcctgttt
     1021 taccggtttt ccgtcagaaa cgccctgagg ccgttttagc ggtgcgtaca atacttgagt
     1081 tatgttaaat gcacctaaga tttggatgat tgtttcatct cgttacaact actgttgtct
     1141 ttttaaccta tatcgtttta tactcgtaat atgctccaat tacactcgac aatggaggtg
     1201 tttgggtgta agatacaatc tggagcccat tacgaggtgt ggtgggtaaa ctggagattt
     1261 cacctgtgag cgagttaact caaaactgct cctcgacctc caggaacagt gaggactgca
     1321 ccgacttcga agaactgcct cctaaagagc tttctttgaa ggattggttc tcagtcttta
     1381 ctgaggtcga tgagtaataa ccaccatgga ggagttttca caacctcctc catggtgggg
     1441 ataacctgct tgcggagaag agataaggct aagccttatc caaattagga gtatcataag
     1501 tatatgaaac ttaatttatt tataggtaga gatatataaa taccaacggt cctaatggtg
     1561 aagaatgtgc agcatagagt acaaggatga catgctggcc cctcggagat tagttatgaa
     1621 aaatgtacgt atgcaattcg atctacctga agataggttg gatgaactcg attctttaat
     1681 gaagaagtgc ggcataagta ctaaaaaaga gttgttcaac tacgcgttaa caatgcttga
     1741 gtgggctgtg gacgagtccg aaagtggtca cgaaatcgca gctatcgata gagacagtaa
     1801 gcaattttat gctctacgaa tgcctattct taagcgagtg aaccgtacat ctactgcaaa
     1861 ttaacttatc acttgatgtg ttaaggagaa ggccagctta aactggcctt ctttttttat
     1921 tcaagagggt ttgattgtgt ccgatagcct agatggattt actgaaggcg atacctcaaa
     1981 agaaaaacaa gcggtaaaac aacccaagaa gttctttagt gacgtcgacg attcagcaga
     2041 tagacggcat ctgagatgga caaaaagtgg cgttactctg gctatgacaa ttttggttgc
     2101 tgtaatgaac tggatggtca taagcatgct atatcacgta gttgatgaag agctttctct
     2161 gatacgaagt ggctttatgc aagcagacgc aaggactgta actactagtg tttatttggc
     2221 tttgattgct ggaacagttg ctgaggtatc tgctttattt ttgataattg taaagtcagt
     2281 ttttaagaac aataacaatg atatatgaag ttgattccag tcgttagatg tagtgtcaca
     2341 catggttttg ttctgtctgt cttgagatca ctctaatcaa aatgtaatct cttgggactg
     2401 aaacgaaaaa accacccggc aaggtggttt tttcgaaggt tcattaaccg gcaagtttct
     2461 gaaccgtggt aactggcttt cgcagagcac agaaaccgaa ttcgttttaa cagtgtagcc
     2521 gtagtctgcc ttacacttca acacctcaac gcttctttgt tctgcgaatc tgttacctgt
     2581 ggctgctgcc attggcgttt taacgcgttt tttccgggtt ggactcaagg tgatagttac
     2641 tggaaaaaac gcagcagtcg gactgaacgg ggggttcgtg catacagtcc agcttggagc
     2701 gaactgcctt cccggatctg agtgtcaggc gtggagtgag aaaacgcggc cataacagcg
     2761 gaatgacacc ggtaaaccga aaggcaggaa caggagagcg cacgagggag ccaccagggg
     2821 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca ccactgattt gagcgtccga
     2881 ttctgtgatg cttgtcaggg ggggcggagc ctatggaaaa acgccccgcc ggggcgtggc
     2941 cttttagccc ttgtccttag ctttcgttat gcttaccgtt acgttat
//
