LOCUS       NZ_CP135158             9108 bp    DNA     circular CON 01-FEB-2024
DEFINITION  Acinetobacter pittii strain AR3676 plasmid pAR3676_7, complete
            sequence.
ACCESSION   NZ_CP135158
VERSION     NZ_CP135158.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37485474
            Assembly: GCF_036320505.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter pittii
  ORGANISM  Acinetobacter pittii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 9108)
  AUTHORS   Hua,X. and Yu,Y.
  TITLE     Genetic Characterization of Plasmid-Borne blaOXA-58 and blaOXA-72 in
            Acinetobacter pittii in China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9108)
  AUTHORS   Hua,X. and Yu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2023) Sir Run Run Shaw Hospital, Zhejiang
            University, Qingchundong 3 RD, Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/31/2024 23:07:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,919
            CDSs (total)                      :: 3,824
            Genes (coding)                    :: 3,678
            CDSs (with protein)               :: 3,678
            Genes (RNA)                       :: 95
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 146
            CDSs (without protein)            :: 146
            Pseudo Genes (ambiguous residues) :: 0 of 146
            Pseudo Genes (frameshifted)       :: 51 of 146
            Pseudo Genes (incomplete)         :: 93 of 146
            Pseudo Genes (internal stop)      :: 23 of 146
            Pseudo Genes (multiple problems)  :: 18 of 146
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP135158.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9108
                     /organism="Acinetobacter pittii"
                     /mol_type="genomic DNA"
                     /strain="AR3676"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:48296"
                     /plasmid="pAR3676_7"
                     /geo_loc_name="China: Shaaxi"
                     /lat_lon="33.04 N 107.01 E"
                     /collection_date="2022"
                     /collected_by="Sir Run Run Shaw Hospital"
     gene            complement(join(9069..9108,1..92))
                     /locus_tag="RQL83_RS19535"
                     /old_locus_tag="RQL83_19595"
     CDS             complement(join(9069..9108,1..92))
                     /locus_tag="RQL83_RS19535"
                     /old_locus_tag="RQL83_19595"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_261632396.1"
                     /translation="MCYNGFSQINELTTELTTELTTELTTELTTELTTELTTELTTG"
                     /name="hypothetical protein"
     gene            complement(196..420)
                     /locus_tag="RQL83_RS19540"
                     /old_locus_tag="RQL83_19600"
     CDS             complement(196..420)
                     /locus_tag="RQL83_RS19540"
                     /old_locus_tag="RQL83_19600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005155986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002072934.1"
                     /translation="MPYEEFQRLIGKSGLSIKEFAALLDMNANSITNYKKNGKVPTTIA
                     VIAVVISDMKDDGLDFYPIFEKVRAYSDQ"
                     /name="hypothetical protein"
     gene            569..811
                     /locus_tag="RQL83_RS19545"
                     /old_locus_tag="RQL83_19605"
     CDS             569..811
                     /locus_tag="RQL83_RS19545"
                     /old_locus_tag="RQL83_19605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001015364.1"
                     /translation="MNHFVQPKLLISSQAKKIGLALINDLELCITARLAFYLDVNPSYD
                     GHEATFIWIEYFLQDHPQAEFSEVREAFLQLIPGC"
                     /name="hypothetical protein"
     gene            complement(884..2083)
                     /locus_tag="RQL83_RS19550"
                     /old_locus_tag="RQL83_19610"
     CDS             complement(884..2083)
                     /locus_tag="RQL83_RS19550"
                     /old_locus_tag="RQL83_19610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004762678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002072939.1"
                     /translation="MTPKILCIKSLELEVEKIMSIQEFAFQLSKKIQEQHSVKISRSHI
                     YELIALDQDYRTYSSFVAQNLLLDAQYDDSEEYYQHELVDALTLEILENPPETDYLNYS
                     EDDLHWDDYEGNVLLEQIRKIIIRLKVLLKLDASEEFYLSISKTVYRELLWLNLEVINF
                     NAVREELSYINFENGKVDDLELGGDYFEFSKIGAIFEKILSYAQDRENADAYALLGAYY
                     RYLANQIAPYGRHGSNFGSRWDNNKQKYINSDETKKNKEKYEEYIKQAEHFESYIKNSP
                     MNLHEINLDADKETVYNQILYLCNRGDTSAIEYFLYEEIFKNHGEAWLYIYLAQMCGVD
                     FTQDDFRDYNAYTGEDYDDYGPIEIAGREAIQYAIDLSELDVDKDNLARKIAAELFEKI
                     "
                     /name="hypothetical protein"
     gene            complement(2308..3084)
                     /locus_tag="RQL83_RS19555"
                     /old_locus_tag="RQL83_19615"
     CDS             complement(2308..3084)
                     /locus_tag="RQL83_RS19555"
                     /old_locus_tag="RQL83_19615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004762676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000191151.1"
                     /translation="MTQEKAKKRGRPAQLLQMAELHAFVEFLLEKDPRSELQNQVIAAL
                     QAEDFNFEMLSEAQQILVKEALKPYREHLKLQLLFDELVITPGRTEYEEKFLELYQDYK
                     KDDLDPAEMNILKTMCTRYLNFKAQKLEYSDLELYLSQIQKKYAGKKRKAENQRKFEVG
                     GGVLSLYKVKDDENLDSKKFLKRIEIDRFLAGKFRQSQLYKKVWALERPNTEKYQLLFE
                     VLHQLSGYSLKGQTLTLLDLVIINAQKKIKSRSEKE"
                     /name="hypothetical protein"
     gene            3391..4551
                     /locus_tag="RQL83_RS19560"
                     /old_locus_tag="RQL83_19620"
     CDS             3391..4551
                     /locus_tag="RQL83_RS19560"
                     /old_locus_tag="RQL83_19620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002011489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_000179382.1"
                     /translation="MASYHFSVKSKNKGYATGHYLYISRLMQYEGIRKTSNETIEHIEP
                     GRCMPAFVKDPIEFWQAADSHERANAKAYMEYEIALPNEFTPEQRKTLIETFLDKHIVP
                     QQYPHSYAIHNVKSRISGEDQPHCHLMFSLKADDGIERSAEQYFKRYNPQDPAKGGAKK
                     IQLQDGHENYSTFLIYIRKQWENHLNDALAQHCPTVTYTLDGQDITIKNQVSADSYEKY
                     NEIHSTLYLPEPKLGVGQQNATAEYLAQIQKIREHNQQERELEERQKQLDQQHEYFYSA
                     ENHPFSSLEVMYYLNYLIKNAKNKEKLEKDFSEIIKIQEPILHQEGWNLNNMNRKLRDS
                     EHNSTPRFEFEVHSQPNYPLPMQNKPPQPEQPKPKSDDYDYGGPGF"
                     /name="MobA/MobL family protein"
     gene            5099..5980
                     /gene="repM"
                     /locus_tag="RQL83_RS19565"
                     /old_locus_tag="RQL83_19625"
     CDS             5099..5980
                     /gene="repM"
                     /locus_tag="RQL83_RS19565"
                     /old_locus_tag="RQL83_19625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004734502.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_005804946.1"
                     /translation="MNKNHVVKSNQVIEASYQLSAVEQRIVLAAISRIPKNQPITDDEL
                     YPVSVDELKQLGVHEKTAYRDLKEGINRLYERSINLSIDDKSIKMRWVQEIQFLDSQSV
                     IGIRFSKPILPFISNLSREFTKYALSDIAGINSGYGIRIYELLVQYRQIGKREISVESL
                     RTMLELGKKYPLFADFKKRVIDTAIDQINEYSPLKVTYEQKKTGRKVTHIIFSFKEKSK
                     SITHTSKDFYQLSDAQINLFGNQLSRLHELSHLASEGESYEILAAKIKDMLRNPHQQQQ
                     FLPHLKNLGFKA"
                     /name="repM"
     gene            6044..6610
                     /locus_tag="RQL83_RS19570"
                     /old_locus_tag="RQL83_19630"
     CDS             6044..6610
                     /locus_tag="RQL83_RS19570"
                     /old_locus_tag="RQL83_19630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_000858429.1"
                     /translation="MKTSTKYSVSDAATLYKKSRATLYKDIKNGVLSRDHDGSIDFSEL
                     LRVYGEPFSGKHSKHTDTSPTQTINTPEYTDNTVIELKNQIDFLKKQLEKAEDREAKAN
                     SRIDTLLTLIEMKPTQVHPTPTSDQSSVQSSPSQVEPTLNVDNDKHDGLTTPEKTENKR
                     IPVPEPVEPEQPKRGWLSRFFLPNG"
                     /name="plasmid replication DNA-binding protein"
     gene            6961..7278
                     /locus_tag="RQL83_RS19575"
                     /old_locus_tag="RQL83_19635"
     CDS             6961..7278
                     /locus_tag="RQL83_RS19575"
                     /old_locus_tag="RQL83_19635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005065053.1"
                     /translation="MLGENHHFLKKFSISDAAIAYKKPRSTILKYIKSGDFELDYNKLI
                     EYSQLVRKFGELTYPIQDEREQQIKKLKDEVMHLKKQLNFFKYTSISNKNRLLISHKHS
                     I"
                     /name="hypothetical protein"
     gene            7803..8324
                     /locus_tag="RQL83_RS19580"
                     /old_locus_tag="RQL83_19640"
     CDS             7803..8324
                     /locus_tag="RQL83_RS19580"
                     /old_locus_tag="RQL83_19640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000999915.1"
                     /translation="MNDSDDRNHKVILEYLQKVLERERDRFYKLDDKVSKLFTTVSTVI
                     TGFLIIVNILLDHNKISIFCSSEITILSLLSLTTFCFLCMSWNFLFKVFNLKKTAHLQH
                     QGFWKYYKNREEIDFYERIIKDHETVIDTYIESNDQKVLLLKKSLSNIYLSAIMFVILV
                     IIYLIFKYLK"
                     /name="hypothetical protein"
     gene            8321..8467
                     /locus_tag="RQL83_RS19585"
                     /old_locus_tag="RQL83_19645"
     CDS             8321..8467
                     /locus_tag="RQL83_RS19585"
                     /old_locus_tag="RQL83_19645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000192351.1"
                     /translation="MTQKNSNTSTNPANQQPHKDIKSEVPPPPVRYGLDEKIKAEVPKS
                     NKK"
                     /name="hypothetical protein"
     gene            complement(8515..8805)
                     /locus_tag="RQL83_RS19590"
                     /old_locus_tag="RQL83_19650"
     CDS             complement(8515..8805)
                     /locus_tag="RQL83_RS19590"
                     /old_locus_tag="RQL83_19650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family toxin"
                     /protein_id="WP_000176412.1"
                     /translation="MTLTIRYKKQFKKDFKRVSSNPKLKKGLLILKDLIDEHLVKTGTV
                     PDEYLPHPLIGEWKPHMECHILPDLLLIWDVDEDNNELILARIGSHSELFG"
                     /name="type II toxin-antitoxin system YafQ family toxin"
     gene            complement(8789..9064)
                     /locus_tag="RQL83_RS19595"
                     /old_locus_tag="RQL83_19655"
     CDS             complement(8789..9064)
                     /locus_tag="RQL83_RS19595"
                     /old_locus_tag="RQL83_19655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005140357.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="WP_005140357.1"
                     /translation="MNTLAKKDEYVRARVPNELKVQAERILKEVGLNTTDAIRLLLTQV
                     VNRGGFPLELRTPNRTTIEAMNSVAEPKKFSSAQELFTDLEDDTDD"
                     /name="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
     Typing          5100..5978
                     /name="rep_cluster_1364"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="85.922"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          3392..4482
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.978"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tcagtagtca gttcagtagt cagttcagta gtcagttcag tagtcagttc agtagtcagt
       61 tcattgattt ggctaaagcc attgtagcac acatgtgcta cacaacaact aacttaacat
      121 aaaatctcat ctacgaaatg cagcagcacg tgctaggatc caattaaatc tttaattggg
      181 ttcacatcaa atactctatt gatcactata agctcgaact ttctcaaaaa ttggataaaa
      241 gtccagacca tcatccttca tatctgaaat tacgactgca attactgcaa tcgtagtggg
      301 cactttaccg ttctttttat agttggtaat tgaatttgcg ttcatatcta aaagcgctgc
      361 aaattcttta atagaaaggc cagacttacc tataagtctt tgaaattctt cgtaaggcat
      421 agttttaaaa gcagaattgt tctgatggaa aagtatagca cagatcattt atttacattt
      481 aaacacataa ataatgtgtt taacacatta tttatgtgtt taaatggtct tatcaagcaa
      541 ttttactctt ttgataggac ttgtttttat gaaccatttc gtacagccca agcttcttat
      601 ttcttctcaa gcaaaaaaga ttggattggc attaattaac gatcttgaac tttgtataac
      661 agcacgattg gctttttatc tggatgtaaa tccttcctat gacggccatg aggcaacatt
      721 catttggatc gaatattttt tacaagatca tcctcaagcc gaattttctg aagttagaga
      781 agcctttcta caacttatcc caggatgtta aacgatgata atgaaatctg tattttatga
      841 gtcaaataca gatttcaaat tctctttttt ataagactat gaattaaatt ttctcaaata
      901 actctgcagc aatcttccta gctaaattat ctttatcaac atccagttct gataaatcta
      961 tagcatattg aattgcctca cgacctgcga tttcaatagg tccatagtca tcataatcct
     1021 cacctgtata agcattgtag tctctaaaat catcctgcgt gaaatcaact ccacacattt
     1081 gagctaagta gatgtataac catgcttctc catgattttt gaaaatttct tcatacagga
     1141 agtattcaat agcactcgta tcacctcgat tacacaagta cagaatctgg ttgtaaactg
     1201 tttccttgtc tgcatccaaa ttaatttcat gaagattcat aggtgaattt ttaatataag
     1261 attcaaagtg ttccgcttgc tttatgtatt cttcatactt ttctttattt ttcttagtct
     1321 catcagaatt gatatatttt tgtttattat tgtcccaacg acttccaaaa tttgagccat
     1381 gtcgaccata aggggctatc tgatttgcta aataacgata gtaggccccg agtagagcat
     1441 aagcatcagc attctcacga tcttgcgcat aagataaaat cttctcaaaa atagccccaa
     1501 tttttgaaaa ctcgaaataa tcacctccaa gttctaggtc atccacctta ccattttcaa
     1561 aattgatata acttaattcc tctcttacag cattgaagtt aataacttct aaattaagcc
     1621 ataaaagttc tcgatagact gttttagaaa tcgacaaata aaactcttct gaggcatcta
     1681 acttaagtaa aaccttaagt cttataataa tttttctaat ctgctccaat agaacattac
     1741 cttcataatc atcccaatga agatcatctt cactataatt tagataatct gtttcaggcg
     1801 gattttccaa tatttctaga gttagagcat caaccaattc gtgctgataa tactcttcag
     1861 aatcatcata ttgagcatca agtaaaagat tttgagcaac aaaagagcta taggtcctat
     1921 agtcctgatc taaagcaatt aattcataaa tatgtgaacg gctaatcttt acagaatgct
     1981 gttcctgaat ttttttagat aattgaaatg cgaattcttg aatagacata attttctcta
     2041 cttccaactc taaactcttt atacaaagaa tctttggtgt cataagactt ctagcaacgc
     2101 atgtttaaat gctaagaacc aaacgagttg atacttaaga aaattagatt ttagatatgg
     2161 ctttaaaaat taaacctatg gttttttatc ccttttctac tcatgcgtaa gtacggtagg
     2221 cgaccttatt tttaaaataa taaatttcgc ataaatttac aattgccaaa atagaaaagt
     2281 cagctaaaaa gcggacagtc aaaaaattca ttctttttca gacctacttt ttatcttttt
     2341 ttgagcattt ataatcacta aatcgagaag cgttaaagtc tgccctttta ggctatatcc
     2401 agacagctga tggagaacct caaaaagtag ctgatacttt tctgtatttg gtcgctctag
     2461 tgcccaaacc tttttataaa gttgagattg acgaaatttc ccggcaagaa aacggtcaat
     2521 ttcgattctt tttagaaact ttttactgtc taaattttca tcatctttta ctttataaag
     2581 acttaaaacc ccaccaccca cttcaaactt acgttgattt tcagcctttc gctttttccc
     2641 ggcatacttt ttctggatct gactcaaata cagctccaag tcactgtatt caagcttttg
     2701 cgctttaaaa ttcagataac gcgtacacat cgttttcagg atattcattt cagccggatc
     2761 tagatcatct tttttataat cttggtacag ctccaggaat ttttcttcat actcagtcct
     2821 tcctggagtt atcaccaact catcaaacaa caactgaagt ttcaaatgct cacggtacgg
     2881 ttttaacgct tctttcacca gaatctgctg agcttctgac aacatttcaa aattaaagtc
     2941 ttcagcttgt agtgcagcaa taacctgatt ttgcagttca gagcgaggat ctttttctaa
     3001 caggaactca acgaaagcgt gtaactctgc catttgtaaa agttgagctg gtctaccacg
     3061 cttttttgct ttttcttggg tcatatcatc tgccgcctaa ttttcagaac tgctaccgat
     3121 ttaaatatgg ttttaccata tttaaatcgg gcagtctgaa aataattatt taaattaaaa
     3181 ataaaatttc ttttcaagaa attttatttt taatttaaat aattcgcact tgacagtttc
     3241 ttctaatcga agaaactagt acgcaatggg gcgattcccc cttgaccccc tttaaccgga
     3301 tttctcagcc attcaggcat ttcaaaatcc ggacggtttt cattccgttt cactgcataa
     3361 aaaccggtcc ccaagccacc agaggtgaac atggccagct accatttcag tgtaaaaagc
     3421 aaaaataaag gctatgcgac cggccactac ctctatattt cccggctgat gcaatatgaa
     3481 ggcattcgaa aaacaagcaa tgaaaccata gaacatattg aacctggacg ttgcatgccc
     3541 gcctttgtca aagatccgat tgagttttgg caggcagccg atagtcatga acgagccaat
     3601 gccaaggcct acatggagta tgaaattgcc ctccccaacg agttcacgcc agaacagcgt
     3661 aaaacgctca tagagacgtt tttagacaaa cacatagtcc cacagcaata tcctcactca
     3721 tacgccatac acaacgtaaa atcacgcatc agtggcgaag atcagcctca ttgtcacctg
     3781 atgttctcgc taaaagcgga tgacggcatt gaacgcagcg cagaacagta cttcaaacgc
     3841 tataaccctc aagatccagc caaaggcggt gctaaaaaaa tccaacttca agatggccat
     3901 gaaaactatt ccactttttt gatctatatc cgaaaacaat gggaaaatca tttgaatgat
     3961 gcccttgcac agcactgccc gaccgtcacc tacacactgg atggccaaga catcaccatc
     4021 aaaaatcagg tttcagcaga cagctatgaa aaatataacg agatccacag cacgctatac
     4081 cttccagagc caaaactagg cgttggccaa caaaatgcga cagcggaata tctcgctcaa
     4141 atccaaaaaa tccgagaaca caaccagcaa gaacgagaac tcgaagaacg ccaaaagcaa
     4201 ttagatcaac aacatgaata tttttatagt gctgaaaacc atccattttc atcattggaa
     4261 gtgatgtatt acctgaatta cttaatcaaa aatgcgaaaa ataaggaaaa actggaaaaa
     4321 gattttagtg agattatcaa aattcaagag cctatactgc accaagaagg ctggaacctg
     4381 aataacatga atcgaaaatt gagagattca gaacataact caacgccacg ctttgagttt
     4441 gaagtccatt cccagccaaa ttatccatta cccatgcaaa ataagccccc tcaacccgaa
     4501 cagccaaagc caaaatctga tgattatgat tacggtggtc ctggttttta aaattatctg
     4561 gctttattca ggccttacag agcgagtgtc tacgagcgaa cagacccaat taaaattttc
     4621 gccatgcttt ttccctgatt acaaactccc ctttgctgct gagactgaga tacagagcat
     4681 cccggagggt gcgaacagat ttaatttgca tctaggtcga gcgaagcgag tataaaaaac
     4741 ttatgagcga agcgaattcc gagttgcttt tgcttttttg aagttatcac gagattaacc
     4801 caaaaattgc cccttcgaac tgagcgtaag cgaagttcaa tagagtttga gcgtagcgaa
     4861 aacatagggc aatttttcat tctttccttt ttaatatatt ttaaatcttt taaatctttt
     4921 cagtcgtctc tgtgccttat atagcaaagg tttcaacgtt taaatatgac ggattgacta
     4981 ctaactatga cggattgact actaactatg acggattgac tacttaacta tgacggattg
     5041 actactaact atgacaagta tatatttgtc atatattaga aaaatcaatg gttttgtttt
     5101 gaataaaaat cacgttgtaa aatcaaatca agtaatagaa gcatcgtatc aattaagtgc
     5161 cgtagagcaa cgaattgtct tagctgccat ttcacggatt cccaaaaatc agcctattac
     5221 agatgatgag ttataccctg ttagcgtcga tgaattaaaa caattgggcg tacatgagaa
     5281 aactgcatac agagatttaa aggaaggtat taatagactc tatgagaggt ctattaacct
     5341 cagtattgat gataaatcta taaaaatgag atgggtgcaa gaaatccaat ttttagatag
     5401 tcaaagtgtc attggaatcc gtttttcaaa acccattctg ccattcattt ccaatctaag
     5461 tagagaattc accaaatatg ccttatcaga cattgctggg atcaatagtg gatacggtat
     5521 tcgtatttat gagctactgg ttcaatatcg gcaaataggt aagcgtgaaa tttctgttga
     5581 gagtttacgg acaatgttag aactcggtaa aaagtaccca ttattcgctg atttcaagaa
     5641 acgagtaatt gacactgcta tagaccaaat caatgaatat agccctttaa aagtcactta
     5701 tgagcagaaa aaaacagggc gtaaagtcac tcatattatc ttttctttta aagaaaaatc
     5761 taaatccata acacatacct cgaaagattt ttatcagcta tctgatgctc aaattaatct
     5821 attcgggaat caattatcac gtttacatga gctttcccat ttagcctcag aaggtgaaag
     5881 ttatgagatt ttagctgcta aaattaagga tatgcttaga aaccctcacc aacaacagca
     5941 gtttttgcca cacttaaaga acttagggtt taaagcttga gtgtattgaa gaatacactt
     6001 attgtattct tcaatacgta tcctaaatac acagcaatac actatgaaaa caagtacaaa
     6061 atacagtgtt agtgatgcag ccacactgta taagaaatct cgtgctacac tttataaaga
     6121 tataaaaaat ggggtgttaa gtcgtgatca tgatggctct atcgacttct ctgagttact
     6181 ccgcgtttat ggtgaacctt ttagtggaaa acactcaaaa cacactgata cgtctccaac
     6241 acaaactata aatacacctg aatacacaga caatacagtt attgaactaa agaatcagat
     6301 cgattttcta aagaaacagt tagaaaaagc agaagatcgc gaagctaaag caaattctcg
     6361 tattgataca ttacttaccc taattgaaat gaagccaact caggttcatc ccacaccaac
     6421 ttcagatcaa tcttcagtgc aatctagtcc atctcaagta gaacctacat tgaatgtaga
     6481 taacgacaaa catgacggat tgactactcc ggaaaaaacg gaaaataagc gtattccggt
     6541 gccggagccg gttgaaccgg agcagccgaa aagagggtgg ttaagccggt ttttcttgcc
     6601 gaatggctaa gggtactatt caactgagct aatcaaagtt gtaaggattt gattaaagaa
     6661 tagccatatt acgtgaaaga tcacaaaaaa aagcccgtac agggatgttc gggctataaa
     6721 ctttgaaata agtatatgaa tttcaaggta aattatgaca catttcgtat aaggtgtatt
     6781 atgttaataa tataaatttt taaaagcata ttgatttaat aatagaaaaa ttatcagagt
     6841 agggcatgtg taaagtaaac gttcgattat taaccaataa tgattaaaga tttatatctt
     6901 gtatgtgtta attttattaa catatatata cgaaattaat aatgccttaa gtgaaatagt
     6961 atgctaggtg aaaatcatca ttttttaaaa aaattttcga tcagtgatgc tgctatagcg
     7021 tataaaaaac ctcgctcgac tatacttaaa tatataaaaa gcggagattt cgaacttgac
     7081 tacaataaat tgattgaata cagtcagtta gttagaaaat ttggtgaact tacttaccct
     7141 atacaggacg aaagagagca acaaatcaaa aaactaaaag atgaagttat gcatttaaaa
     7201 aaacaactta acttttttaa atatacttca atctcaaaca aaaatagact attaatttct
     7261 cataaacatt ccatttaaca taatgggcgt tatccgaaat cgtcttttat aagttaaata
     7321 aaatcatgta cttagctatt ttacataacc cgaaactacc gcaaaaatcc gctttgtttc
     7381 caaagcggat tttttttgag caaggttgat acttcttgcc aataaaattg gttaaataat
     7441 gatcaaaaat agcaattcca tagagttttt aagccttttt tacccaaaag aatccacaat
     7501 ttatgcggat aaccattggg ataaattttg attatttttt gtacgttttg gcttacagcg
     7561 ttttacgaaa ttgtctgtat ctatcccccg atgaagtcct tatgatcaat ctatatgaac
     7621 aagatgttca atgacttaaa acaacaataa taacaataat aatcagtcag gcaccatgac
     7681 catcaggtag tcagccctac ctaggcgctc cacaccgaaa aatcccgaca tgatgtcggg
     7741 attttttatt gttaaaattt aaattaaata aattttgtta tttgaaataa aggggattga
     7801 gtatgaatga ctctgatgat cgtaatcata aggtaattct tgagtatcta cagaaagtac
     7861 tagaaagaga gcgagatcga ttttataaat tagatgacaa agtatctaag ttatttacga
     7921 cagtatctac tgtgattaca ggatttctaa ttatagttaa tatcctttta gatcataata
     7981 aaattagtat tttttgttct tccgagatta ctattctaag tttattgtca cttacaacat
     8041 tctgcttctt gtgcatgtct tggaacttcc tttttaaagt ttttaattta aaaaaaacag
     8101 ctcaccttca acatcaaggg ttttggaaat attataaaaa tagagaagaa atagattttt
     8161 atgaaagaat cattaaggat catgaaactg ttatagatac atacatagaa tctaatgacc
     8221 aaaaagtttt acttttaaag aagtctttaa gcaacatcta tttatcagca ataatgtttg
     8281 ttatattagt tattatctat ttgattttta agtatctaaa atgacccaaa aaaattccaa
     8341 tacaagtacc aacccagcta atcaacaacc tcacaaagat atcaaatcag aagttccacc
     8401 tcctcctgtt agatatggtt tagatgaaaa aattaaagct gaagttccaa aaagtaataa
     8461 aaaataatct ctaattattg acaacaaaaa gcccgaatat atcgggcttt ttacttatcc
     8521 aaaaagttca gaatgagatc caatccgagc aagtattagc tcattattat cttcatccac
     8581 atcccaaatt aataataaat caggtaaaat atggcattcc atatggggtt tccactcacc
     8641 aatcaaagga tgaggtaaat actcatctgg aacagtccct gtttttacca aatgctcatc
     8701 aataagatcc ttcaaaatta agagtccttt cttaagcttt gggtttgagc ttacccgttt
     8761 aaaatctttt ttaaattgtt tcttgtacct aatcgtcagt gtcatcttct aaatccgtaa
     8821 acaattcctg agcagaacta aattttttag gttctgcaac agagttcata gcctcaattg
     8881 ttgtacggtt aggggttctt aactctaaag gaaatccacc tcgatttact acttgcgtta
     8941 ataataagcg aatagcatct gtagtattta aacccacttc ttttagaatt cgctccgctt
     9001 gaacctttaa ttcatttgga accctagcac gtacatactc atcttttttt gccaaagtat
     9061 tcatatgttt atccagtagt cagttcagta gtcagttcag tagtcagt
//
