LOCUS       NZ_CP135157            10555 bp    DNA     circular CON 01-FEB-2024
DEFINITION  Acinetobacter pittii strain AR3676 plasmid pAR3676_6, complete
            sequence.
ACCESSION   NZ_CP135157
VERSION     NZ_CP135157.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37485474
            Assembly: GCF_036320505.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter pittii
  ORGANISM  Acinetobacter pittii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 10555)
  AUTHORS   Hua,X. and Yu,Y.
  TITLE     Genetic Characterization of Plasmid-Borne blaOXA-58 and blaOXA-72 in
            Acinetobacter pittii in China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10555)
  AUTHORS   Hua,X. and Yu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2023) Sir Run Run Shaw Hospital, Zhejiang
            University, Qingchundong 3 RD, Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/31/2024 23:07:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,919
            CDSs (total)                      :: 3,824
            Genes (coding)                    :: 3,678
            CDSs (with protein)               :: 3,678
            Genes (RNA)                       :: 95
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 146
            CDSs (without protein)            :: 146
            Pseudo Genes (ambiguous residues) :: 0 of 146
            Pseudo Genes (frameshifted)       :: 51 of 146
            Pseudo Genes (incomplete)         :: 93 of 146
            Pseudo Genes (internal stop)      :: 23 of 146
            Pseudo Genes (multiple problems)  :: 18 of 146
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP135157.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10555
                     /organism="Acinetobacter pittii"
                     /mol_type="genomic DNA"
                     /strain="AR3676"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:48296"
                     /plasmid="pAR3676_6"
                     /geo_loc_name="China: Shaaxi"
                     /lat_lon="33.04 N 107.01 E"
                     /collection_date="2022"
                     /collected_by="Sir Run Run Shaw Hospital"
     gene            complement(join(10379..10555,1..105))
                     /locus_tag="RQL83_RS19465"
                     /old_locus_tag="RQL83_19525"
     CDS             complement(join(10379..10555,1..105))
                     /locus_tag="RQL83_RS19465"
                     /old_locus_tag="RQL83_19525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985610.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antidote protein"
                     /protein_id="WP_049066984.1"
                     /translation="MAIKLRKWDSAEHLKTEEDMQAYLQVCIEESNGDAAFIAKALGNI
                     AKAKGMAQLSRDTGLGRESLYKALSGDVNPSFDTVIKVVKALNLRLAI"
                     /name="addiction module antidote protein"
     gene            complement(106..408)
                     /locus_tag="RQL83_RS19470"
                     /old_locus_tag="RQL83_19530"
     CDS             complement(106..408)
                     /locus_tag="RQL83_RS19470"
                     /old_locus_tag="RQL83_19530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286964.1"
                     /GO_function="GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /GO_process="GO:0008219 - cell death [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_049066985.1"
                     /translation="MYSIYTTEVFDDWFTKLKDQQAKRRIQVRIDRVEDGNFGDTEPVG
                     EGVSELRFFFGPGYRIYYCKQGQRVVILLSGGDKSTQSKDIKLALQLAQDLEEEL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            553..981
                     /locus_tag="RQL83_RS19475"
                     /old_locus_tag="RQL83_19535"
     CDS             553..981
                     /locus_tag="RQL83_RS19475"
                     /old_locus_tag="RQL83_19535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016653412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_030423737.1"
                     /translation="MKYSNEKIVKALLLSPLPLLFFTAVLFIVMNQEYSLYSILVVLVG
                     HGLVYLAYSILTVPFSFIFSVLLNRYNSLNLLTICIASIIIATPFFILFGWSHTGEISK
                     EWWKMYTDTWTIFMALFPGLCYWLFLINLKDKKSKNIE"
                     /name="hypothetical protein"
     gene            complement(1187..1330)
                     /locus_tag="RQL83_RS19480"
                     /old_locus_tag="RQL83_19540"
     CDS             complement(1187..1330)
                     /locus_tag="RQL83_RS19480"
                     /old_locus_tag="RQL83_19540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004676226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004676226.1"
                     /translation="MKKLKEILQGIAFLFIGIPIMIMASIMDQRAFKKEQDQRQKAQDQ
                     SK"
                     /name="hypothetical protein"
     gene            complement(1352..1906)
                     /locus_tag="RQL83_RS19485"
                     /old_locus_tag="RQL83_19545"
     CDS             complement(1352..1906)
                     /locus_tag="RQL83_RS19485"
                     /old_locus_tag="RQL83_19545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_004676224.1"
                     /translation="MAKLSLSEVSNRFNVSRSTLYRAIKEGRISRSADGLFDIAEVIRC
                     FGEPIKKHEKKQEVDQSKDDADLRQLVDFMKKEIESYKDREKRYLDQIDRFQLLLGHKE
                     SEEKMSHDTPNRHFNETPCDNNDDSSESIENKSIEPNSLSHDTVTRHTSEAPKNVSQTH
                     HETRKKRSLFGRVLSAVFDED"
                     /name="plasmid replication DNA-binding protein"
     gene            complement(1899..2849)
                     /gene="repM"
                     /locus_tag="RQL83_RS19490"
                     /old_locus_tag="RQL83_19550"
     CDS             complement(1899..2849)
                     /gene="repM"
                     /locus_tag="RQL83_RS19490"
                     /old_locus_tag="RQL83_19550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786597.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_004676222.1"
                     /translation="MADLVVKSNKLVQALQTLTLSETRLLQLAIVDARETGQGLSAEEP
                     LELNASRYATAFNVSPDAAYLALVEAEDSLFKRQFTITNEDGTLTKSRWIQDANYRKGE
                     GRILVTLTRVVIEHVTKIDGFEQYFTSYHLKKTSDFKSVYAVRLYELLMQWKSVGKTPI
                     YELNKFRSQLGIGVNEYDRMEAFKRRVLDIAIKQINELSDITVKYEQHKKGRAISGFSF
                     VFKQKKTNQPIADKRDPNTLDLFSKMTDAQRHMFANKLSELPEMGKYSQGTESYQQFAV
                     RIAEMLQDPAQFKELYPYLKKVGYMPSNKKDLVNG"
                     /name="repM"
     gene            complement(3417..5045)
                     /gene="mobQ"
                     /locus_tag="RQL83_RS19495"
                     /old_locus_tag="RQL83_19555"
     CDS             complement(3417..5045)
                     /gene="mobQ"
                     /locus_tag="RQL83_RS19495"
                     /old_locus_tag="RQL83_19555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005000602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_329777050.1"
                     /translation="MAIYHFSVKAISRSDGRSAVACAAYRSGEKLVCDYYGKEQDYTKK
                     TGVEFTNIYAPENTNPDLLNRQKLWNTVEKVENRKNSLLAREFEIAFPSELNAEQRQAL
                     LSNLCQELVKRHGVIVDAAIHAPHVEGGSDDRNYHAHIMFTTRAISKTGDFESKKYRDF
                     SRDDGTKTVSDWREHFADLVNTQLEQIGSNERVSHLSYADLGNGLEATVHEGYAVTQLR
                     RLGIDTEISLANDAIRQRNAEKTVNEQVIKELDQEITVSERLICDLREEKSEYDRKQAE
                     TQKAATIAAQRKIEHDREQAKQLDRDKFLQLQDRYKNFADSYFITINNKNQVLNDISEQ
                     LERSKKWLSKQRDVYEREGIFYHAMTHDMISINTPEYWLSSVQFDRKKKEIERQYQTQI
                     IELISDSNIEIVVRDLRETAAKILGRGEDLPVNHQEKQTFFKKLFAKKEYVHSYETLSD
                     YDEHVVPVLKKIEIRQELIERQKEKQLEREKLDEIEKKRYEQEVRQIKLENEKRYESER
                     NQRYQSQRDFETEQPKPRPKNDFEI"
                     /name="mobQ"
     gene            5277..5537
                     /locus_tag="RQL83_RS19500"
                     /old_locus_tag="RQL83_19560"
     CDS             5277..5537
                     /locus_tag="RQL83_RS19500"
                     /old_locus_tag="RQL83_19560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005000600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005000600.1"
                     /translation="MSLDYLNEKIAKQEERLKQLKVQKQATIAREKAKQKEQDRKDDTR
                     RKILLGSYLLKKMENEENKEKILAEMNEYLTQERDRKLFNL"
                     /name="hypothetical protein"
     gene            5878..6225
                     /locus_tag="RQL83_RS19505"
                     /old_locus_tag="RQL83_19565"
     CDS             5878..6225
                     /locus_tag="RQL83_RS19505"
                     /old_locus_tag="RQL83_19565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004641894.1"
                     /translation="MQIKFNNANPCMPKNLAKQLIVESAKLLNHQQRQALLDIFMTLDQ
                     AERTAENTLQQHAVAQQSTHNFSQDYECQEYNQEYWSGHYYDSYSQEYPEDWVKRTQST
                     PGRHKFPRTAY"
                     /name="hypothetical protein"
     gene            6721..6855
                     /locus_tag="RQL83_RS19510"
                     /old_locus_tag="RQL83_19570"
     CDS             6721..6855
                     /locus_tag="RQL83_RS19510"
                     /old_locus_tag="RQL83_19570"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_266095202.1"
                     /translation="MLDFSLLANIRPEIETVPLVGVNDALQRLRAGDVKFRFVIQMNA"
                     /name="hypothetical protein"
     gene            6896..8248
                     /gene="gorA"
                     /locus_tag="RQL83_RS19515"
                     /old_locus_tag="RQL83_19575"
     CDS             6896..8248
                     /gene="gorA"
                     /locus_tag="RQL83_RS19515"
                     /old_locus_tag="RQL83_19575"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230226.1"
                     /GO_function="GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]; GO:0004362 - glutathione-disulfide
                     reductase (NADP) activity [Evidence IEA]; GO:0050661 - NADP
                     binding [Evidence IEA]"
                     /GO_process="GO:0045454 - cell redox homeostasis [Evidence
                     IEA]; GO:0006749 - glutathione metabolic process [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="WP_002122354.1"
                     /translation="MSKHYDYIAIGGGSGGIASVNRAAMYGKKCALIEMGDIGGTCVNV
                     GCVPKKVMWYSAQIADAIHKYGPDYGFNTELKSFNWKKLVENRQAYISRIHTSYDTGLA
                     KNNVDVIRGFAKFLDAQTIEVNGETYTADHILIATGTQAFKPEIEGAEYGIDSNGFFGL
                     EHLPKRTAIVGSGYIAVELAGVLNALGSNVQLFIRKHQPLRGLDHFLGKTLLENMRSEN
                     INVHTHSQINKVTKVQDNSLVLHLEDGTEHSVDCLIWAIGRKPATEAINLNKTQVQLNE
                     NRFIHVDAYQNTTQSGIYAVGDITGKVELTPVAVAAGRRLSERLFNNKPNEHLDYENIP
                     TVVFSHPPIGTVGLTEDQAIEKFGKDAIKIYSSSFTSMYTAITEHRQLTKMKLICVGTD
                     EKVVGIHGIGYGMDEILQGFAVALKMGATKKDFDNTLAIHPTSAEEFVTMR"
                     /name="gorA"
     gene            8316..>8420
                     /locus_tag="RQL83_RS19520"
                     /old_locus_tag="RQL83_19580"
                     /pseudo=""
     CDS             8316..>8420
                     /locus_tag="RQL83_RS19520"
                     /old_locus_tag="RQL83_19580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017508710.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family transcriptional regulator"
                     /name="CysB family transcriptional regulator"
     gene            8695..9525
                     /locus_tag="RQL83_RS19525"
                     /old_locus_tag="RQL83_19585"
     CDS             8695..9525
                     /locus_tag="RQL83_RS19525"
                     /old_locus_tag="RQL83_19585"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_049044175.1"
                     /translation="MKILTFLVITLFSCMTAHAAERKWRSLAGVMYLDENSVVVTNEPK
                     LVNATIYVHNSKTNENLLSNTTVDCKDNTYYIDDLIQVYDEKFILKRAILSPINTRQDP
                     KKITSESLYLALVTLCNDSNNERFREKVSNLKSRYDRNSIGQLYNFMGTNEWFLFHGMV
                     SGFDTINVKGCESNNKLENTTDCKSAKGLLQNYGSLNYIPDNYRYQNAINIKQLALSGK
                     LDVLKSIKLYSTWSSSIYKKAESYSRSSQELSRVYALLLFYEDVTGKVVNGIKN"
                     /name="hypothetical protein"
     gene            complement(9846..10181)
                     /locus_tag="RQL83_RS19530"
                     /old_locus_tag="RQL83_19590"
     CDS             complement(9846..10181)
                     /locus_tag="RQL83_RS19530"
                     /old_locus_tag="RQL83_19590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520300.1"
                     /GO_function="GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="WP_004740263.1"
                     /translation="MFLDWPEHTAHVKKSFGSLGKTHPKMLQAYLALNDAAAAEALDIK
                     TRELISIAVAITTRCESCISVHAEAAMKAGATESEVAGALATAIHLNAGAAYTFALRAL
                     EACEAQK"
                     /name="carboxymuconolactone decarboxylase family protein"
     Typing          complement(1936..2843)
                     /name="rep_cluster_1259"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="82.057"
                     /coverage="98.0"
                     /evalue="0.0"
ORIGIN
        1 ggcatcacca ttagattctt caatacatac ttgtaagtaa gcctgcatat cttcttctgt
       61 tttaagatgc tcagcactgt cccatttgcg aagtttaata gccatttata gctcctcctc
      121 taaatcttgt gccaattgca gggcaagttt tatatcttta ctttgcgtag acttatctcc
      181 acctgataaa agaataacaa ccctttgccc ttgtttgcag tagtaaatcc tatagcctgg
      241 tccaaagaag aaacgtaatt cagagacacc ttcaccaact ggttcagtat ctccaaagtt
      301 tccatcttca acccgatcaa ttcggacttg tatgcgtctt tttgcctgtt ggtcttttaa
      361 cttggtgaac cagtcatcaa agacttcagt ggtatatatt gagtacatag gtaaaatgta
      421 tcctatagga tacatttatg caagaaataa acttggtttt gggagcctga aacgctgaat
      481 ttcgtataac cgccattatg ttaaaatcgt tatagtatta ttatttattg agtagataag
      541 agaattaaat tagtgaaata ctcaaatgaa aaaatagtaa aagctctctt actttctcct
      601 ttaccactac ttttttttac agcagtcctg tttatcgtaa tgaatcagga atacagccta
      661 tactcgattt tagtggttct tgtagggcac ggtcttgtct acttggctta ttctattttg
      721 actgtacctt tcagcttcat attctcagtt cttttaaatc gttataacag tcttaattta
      781 ttaactatct gtattgcctc tataattatt gctactccat tttttattct ttttggatgg
      841 agtcacacag gagaaatttc gaaagagtgg tggaagatgt atacagatac atggactatc
      901 tttatggctc tatttcctgg cttatgttat tggctatttc tgattaacct aaaagataag
      961 aaatcaaaga atattgaata aaccattgca tagagattct taaatttccc taaaattaac
     1021 ataatacacc ttatacgaaa tgctctaaat tagttgtttt atttcaataa tttatagtat
     1081 taagtgcagc ctgattctta gcaatcaggc tttttttatg atttttcatg ttccacgcct
     1141 attatttgac caatgttcca cgattttggt tagttttgat gaataatcac ttgctttgat
     1201 cttgggcttt ctggcgctga tcctgctcct ttttaaaggc tcgttgatcc ataatgctgg
     1261 ccataatcat gatcggaatc ccgataaata gaaatgcaat gccttgtagg atctctttca
     1321 gttttttcat acgtaatgct ccttggggag cttagtcttc atcgaatacc gcactcagta
     1381 ctcgcccaaa taagctacgc ttttttcttg tctcatgatg tgtctgtgat acgttttttg
     1441 gtgcttcact ggtatgtctc gttactgtgt catgagataa tgaatttggc tctatgcttt
     1501 tattttcaat actttcactt gagtcatcat tgttgtcgca aggtgtttca ttgaaatgtc
     1561 tatttggtgt gtcatgagac attttttctt ctgactcttt atgtcctagc aacaattgga
     1621 agcggtctat ttggtctaaa tagcgtttct cacgatcttt atatgactca atctcctttt
     1681 tcataaaatc aacaagctga cgtaaatctg catcatcctt agattgatca acttcctgtt
     1741 ttttttcatg ctttttgatt ggctctccaa agcagcgaat gacttctgca atgtcaaaaa
     1801 gaccatctgc gcttcgagat atacgccctt cttttatagc cctatatagc gtagagcgac
     1861 taacattaaa gcgattggat acttcactta gtgataactt agccatttac taagtccttt
     1921 ttatttgatg gcatatatcc cacttttttt aggtatgggt atagctcttt gaattgagcg
     1981 ggatcttgta gcatctcagc aatacgtaca gcaaactgtt ggtagctttc tgtaccttga
     2041 gaatatttcc ccatttcagg taattctgaa agcttattag caaacatatg acgttgagca
     2101 tccgtcattt ttgaaaaaag atctagcgtg tttggatctc gcttatctgc tattggttga
     2161 ttcgttttct tttgtttgaa gacgaatgaa aaacctgaaa tagcacgacc ttttttatgt
     2221 tgctcatatt taacggtaat atcagaaagc tcattaattt gtttaatagc aatatctaaa
     2281 acacgtcttt taaaagcctc catacgatca tattcattaa ctcctatacc caattggcta
     2341 cgaaatttat tcaactcata tataggtgtt ttgcctacag acttccattg cattaacaac
     2401 tcataaagtc gtacagcata aacgctttta aaatcggaag tttttttaag atggtaacta
     2461 gtaaaatatt gttcaaagcc atctattttt gtgacatgtt caattacaac acgagttaat
     2521 gttactagta tgcgaccttc accttttcga taattagcat cttgaatcca acgacttttt
     2581 gttaaagttc catcttcatt ggtaatagta aattgtcttt taaataaaga atcttctgcc
     2641 tcaactaaag ctaaataagc agcatcaggt gaaacattaa aggctgtagc atatctacta
     2701 gcatttagtt ctaatggttc ttctgctgat aatccttggc ctgtttcacg agcatcgaca
     2761 atagcgagct gtaataggcg agtttcactt aaagtaagtg tctgtaaggc ttgaacaagt
     2821 ttatttgatt ttacgactaa gtcggccatg tcattctatt tagcaacggt tttattgtgt
     2881 tgttataaca acacaataaa tatttgttgt caaattccga ggaaacgttg ctatataacc
     2941 gaggaaacgt tgctatataa ccgaggaaac gttgctatat aaccgaggaa acgttgctat
     3001 ataaccgagg aaacgttgct atatacttct taaaagcatt gtaaaataag ggtttcaggc
     3061 catttaaaaa catttaaaaa catttaaaat aattaaaagc ccaaggagtg aaaaattgcc
     3121 ctatgttttc gctacgctca aactctattg aatctcgctt tcgctcgatt cgtcggggca
     3181 attttttggc tcatctcgtt gtgacttcaa taaaaagcaa aagcacgatg aattcgcttc
     3241 gctcataaag cttttttgct cgctacgctc ggtctagggg cgaatttcag catgaaaata
     3301 tagattttct aggattcaag tcatttaaaa gcggtttttc agagggtcga tggtcgaata
     3361 ttgctagttc gctcgtagac actcgctctg tagatatttg ttattgttta gaaaaactaa
     3421 atttcaaaat catttttagg tcttggtttt ggctgttctg tttcaaaatc tcgctgagat
     3481 tgataacgct gattgcgttc tgactcgtaa cgtttttcat tttcaagctt aatttgtcga
     3541 acttcctgtt cataacgttt tttctcgatc tcatcgagtt tttctctttc gagttgcttc
     3601 tctttttgac gttctataag ttcttgtctt atctctattt ttttcaaaac agggacgaca
     3661 tgttcatcat aatcagaaag tgtttcatag ctatgtacat actctttttt tgcgaataac
     3721 tttttgaaaa acgtctgttt ttcttgatga ttaacgggta gatcctcgcc acgccctaag
     3781 atttttgctg ccgtttctct taaatcacgt acaacaattt caatattaga atctgaaatt
     3841 aactcaataa tttgtgtctg atattgccgt tcaatttctt ttttctttct atcaaactgg
     3901 acgcttgaaa gccaatactc aggtgtattg atgctgatca tgtcatgcgt catagcgtga
     3961 taaaatatgc cctctcgttc gtaaacatct cgctgtttcg atagccactt tttcgacctc
     4021 tcaagctgct cagaaatatc attaagcacc tgatttttat tgtttattgt aatgaaatag
     4081 ctatcagcaa agtttttata gcgatcctga agctgaagaa atttatctcg atcaagttgt
     4141 ttagcttgtt ctctatcatg ctctattttg cgttgtgctg cgattgtagc cgctttttga
     4201 gtttcagctt gctttctatc gtactcgctc ttttcttctc ttaaatcgca aataagacgc
     4261 tctgagaccg tgatttcttg gtctagttct ttaatgactt gctcatttac agttttttca
     4321 gcattacgtt gcctgattgc atcattagca aggctgattt ctgtatcaat gccaagtctg
     4381 cgtagctgtg tgactgcgta gccctcatgt acggtggctt ctagcccgtt gcctaggtct
     4441 gcatagctga gatggcttac tcgttcgttg ctgccgattt gctctaactg tgtattcact
     4501 agatcggcaa aatgttctct ccagtcgctt acggtttttg tcccatcatc cctactaaaa
     4561 tcacgatatt tcttgctttc aaagtcacca gtcttactaa tagctctggt cgtaaacata
     4621 atgtgggcat ggtagttgcg gtcatcagat ccaccctcaa catgtggcgc atgtatcgct
     4681 gcatcaacga tcacgccatg ccgtttaaca agttcctggc acaggttaga aagcagagct
     4741 tgccgttgtt cggcattcaa ttcggatgga aaggcaattt caaattcacg ggcaagaagt
     4801 gaattttttc tgttctcgac tttctcaact gtgttccaca atttttgacg atttaataaa
     4861 tcgggatttg tattttcagg tgcatagata tttgtaaatt cgacacctgt tttttttgtg
     4921 tagtcttgct cttttccgta atagtcacat accaattttt caccgctacg ataggcagca
     4981 caagcaacgg cactacgtcc atcacttcta ctgatcgctt taactgaaaa atgatatatc
     5041 gccattttgc ttttgctctt tatttcgatt tatcgaaatt accaacctgt tcaggttggt
     5101 caggggtaaa ggggagtccc ctttcgcacc aaactaatca catacttaaa gtatggattt
     5161 agtttggcta agtgcgccct tacgggattc tttgagtatc atatagcgtc ttgaacttca
     5221 gttcaagcgg gattatgatc gagaagaatc caaaagctta agaacggtat ttgattatga
     5281 gtcttgatta tctgaatgaa aaaattgcaa aacaagaaga gcgattgaaa cagttaaaag
     5341 tgcaaaaaca agcaactatt gctcgtgaaa aagcaaagca aaaagaacaa gaccgaaagg
     5401 atgatacaag acgtaaaatt ttgttgggtt cttacttact caaaaagatg gaaaatgaag
     5461 aaaataagga aaaaatactc gctgaaatga acgagtattt aacacaagaa agagacagaa
     5521 agttatttaa tctttaatgt ttgaatagcc actgatttta tagacacctt ttagttagat
     5581 ttttcagttt ttataagaac tactgattag gaccttgaca ccctactttt tactggataa
     5641 tcttatttca acgcagcaaa atctgtgtcc aggcgtgaga acctgataaa atctaggtgc
     5701 aattaaatgt cgcacttgcg gctatttttt tgcctactgt taagcagtcc ctatggtagc
     5761 ttagcagggc agctttgtgc tggctgatcc tagattcagt attctcaccc ctgttaagtt
     5821 gcctccattg ccgtgagaag tgatggagat aattaaactc ttatctagga aactgctatg
     5881 cagattaaat tcaataatgc aaacccatgc atgccaaaaa acctcgctaa acaactgatt
     5941 gtcgaaagcg caaaacttct gaatcatcaa caacgccaag ccttgctcga tattttcatg
     6001 actttagatc aggcggaaag aacagctgaa aatacacttc agcaacatgc tgtagcgcaa
     6061 caaagcaccc acaacttttc tcaagattac gaatgccaag aatacaatca ggaatattgg
     6121 tcaggtcatt actatgattc ttacagtcaa gaatatccgg aagattgggt aaaacggact
     6181 cagtccaccc caggtcgtca taagttccct agaactgctt actgatcgtg gggggtgcct
     6241 ttttaaaagt aggcatcata atccgagcta ctcgaccgag ttggctcgga tgaataccgg
     6301 ggaaactgag ggatttgaga acctgatgac agtcgttgaa ctgcatatta aagaactcaa
     6361 tacctctgcc gcctaaagca ttcactacac gattaatcgt atcaaaacgt ggtgcttaat
     6421 cctgacgtag gactttgtgg agtgcctcac cccaatccct gcttctttag cgattttgag
     6481 tcatgccacg agctttagca ataacaccaa attcatattc taaaagcgga catttctact
     6541 ttgggctact gaatttcgta tatgagattt tatgttaaat gttttctata agttcgcttt
     6601 ataaccaccc ctaatttgct agcaggttct atataactta gtattcgcaa gtctatttgt
     6661 tattactgta taagtaaaat tcaaggctct ttaacaggaa cacctcacga ttcagaaaaa
     6721 atgctcgatt ttagtctatt agcaaatatt cgtcctgaaa tagaaactgt accgttagta
     6781 ggtgttaatg atgctttaca acgtttaaga gcaggagatg ttaagtttag atttgtcatt
     6841 caaatgaatg cttaagtctt taacttttta aatctaaaaa acaggaattt catttatgtc
     6901 taaacactat gattatattg cgattggcgg aggcagtggc ggtattgcat cagtcaatcg
     6961 tgctgccatg tatggtaaga aatgtgcact gatagaaatg ggtgatattg gtggaacctg
     7021 tgtcaacgta ggatgtgttc ctaaaaaagt gatgtggtat tcagctcaaa ttgccgatgc
     7081 gattcataaa tatggtcctg actatggctt taatacagaa ttgaaatcct ttaactggaa
     7141 gaaattagtt gaaaacagac aagcctatat cagccgaata catacatcgt atgacacagg
     7201 actagctaaa aataatgttg atgtcattcg aggttttgca aaatttttag atgctcaaac
     7261 aattgaagta aatggtgaaa cgtacacagc tgaccatatt ttaattgcta cggggactca
     7321 agcattcaaa cctgaaattg aaggtgctga atatggtatt gattccaatg gtttcttcgg
     7381 attagaacac ttaccgaagc ggactgctat tgttggttct ggctatattg ctgtggaatt
     7441 ggcaggtgtt cttaatgcat tgggttcgaa tgtacaatta tttattcgta aacatcagcc
     7501 tttgcgtgga ttagatcatt tccttggtaa aacactatta gagaatatgc gcagtgaaaa
     7561 tattaacgtg catactcata gtcagattaa caaagtgact aaagttcaag ataactcact
     7621 tgtattacac ctagaagatg gtacagaaca tagtgtagat tgcttgattt gggcaattgg
     7681 tagaaaacct gcaactgaag cgatcaatct taataagact caagttcaat tgaacgagaa
     7741 tcgctttatt catgttgatg catatcaaaa tacaacacag tctggcattt atgcagtggg
     7801 tgatattaca ggtaaagtcg agttaacacc tgttgctgtg gcagcgggtc gaagactgtc
     7861 tgaacgttta tttaacaata agccaaatga acatttagat tatgaaaata ttccgactgt
     7921 ggtgtttagt cacccaccta ttggaacagt tggattaacc gaagatcaag ccatagaaaa
     7981 atttggtaaa gatgccatca aaatttatag ttcttccttt acatccatgt atacagcgat
     8041 aacagaacat cgacaactca caaaaatgaa attaatttgt gtaggaactg atgagaaagt
     8101 ggtcggaatt catggtattg gatacggtat ggatgaaatt ctgcaaggct ttgctgttgc
     8161 tttaaaaatg ggagcaacta aaaaagactt cgataacact ttagcaattc atccgacttc
     8221 tgctgaagaa ttcgtcacaa tgcgttaatc attgatcatc taaaagagca tttatttata
     8281 tatttgctct tatttttata ttctatatcc aaattatgaa ctttcaacaa cttagaatta
     8341 ttagagaact cactcaacat aatctcaatt tatcacaaac agcacatgct ctaaaaacat
     8401 ctcaatctgg tttaagtaaa aaagtcttct ttcattatta tatcttttta tttttctgaa
     8461 atatttactt taagatattg aataacaaaa taaaataaat gacagcttat gtgtcactaa
     8521 ctaatgtcat tgattgtcac taaattctat aactcattga tattatttaa tcagaaatga
     8581 caacttatgt gtcatctgac gaatgttgat aaattagtgg ctatgtaagt gctaatttta
     8641 cacttctcta ttatactttt tatcaaatta tttttaataa tatgctacta cttaatgaaa
     8701 atattaactt ttttggtgat aactctcttt tcatgtatga ctgctcatgc tgctgagagg
     8761 aaatggagat ccttagcagg tgtgatgtat ttagatgaaa attctgtagt tgttaccaat
     8821 gaacctaaac tcgttaatgc aactatctat gtgcataatt ctaaaacaaa cgaaaatttg
     8881 ttaagtaata ctacagttga ttgtaaagat aatacttatt atatagatga cttaattcag
     8941 gtatatgatg aaaaatttat tctaaaacgg gcaattcttt ctccaatcaa tactagacaa
     9001 gacccgaaaa aaattacatc tgagtctctt tatttagctt tagtaactct ttgtaatgac
     9061 tcgaataatg agaggtttag agaaaaagta tctaatttaa aatcacgtta tgatagaaat
     9121 tctattggtc aactttacaa tttcatgggc actaatgaat ggttcttatt tcatggaatg
     9181 gtatctggtt ttgataccat aaacgttaaa ggatgtgagt ccaacaataa actggaaaat
     9241 actacagact gtaaaagtgc aaaaggttta ctccaaaatt atggaagcct caactatatt
     9301 ccagataatt acagatatca aaatgcgata aatattaaac aacttgcgct atctggaaaa
     9361 ttagatgttc taaagtctat taaactctat agcacatggt cttcgtctat ttacaaaaaa
     9421 gccgagtcat acagtaggtc tagtcaagaa ttatctcgtg tttacgcact attgctgttc
     9481 tatgaggatg taacagggaa agttgttaat ggtattaaaa actaataact agcccaatga
     9541 gttgaaagac tgaataggtt tttgaatgat taccgattga aaagatcttt tagcctcact
     9601 aaaattaaca taatacacct tatacgaaat tcaaaaaaaa taatattatc tttatgataa
     9661 ttaaagaaat ttaatataat ctccattata ttaaattctc attacatagg ctaagaactg
     9721 actcaataat aaaaaatggg agttttgttg aactcccatt tttgagactg tacattaaat
     9781 tttttcaaat aattatgtag cagtttttct agtcaaatta tctttttcaa tacctaataa
     9841 agatattatt tctgtgcttc acatgcctct aaagctcgta aagcaaatgt atatgcagct
     9901 cctgcattca aatgaattgc tgtagctaaa gcaccagcta cttcgctttc tgttgcgcca
     9961 gctttcattg ctgcttctgc atgcactgaa atacagcttt cacaacgcgt agtgattgcc
    10021 actgctatag atattaattc acgtgttttg atatctaatg cctcggcagc agcagcatca
    10081 ttaagtgcta aataagcttg aagcatttta ggatgagttt ttcctaaaga tccaaatgat
    10141 tttttgacat gtgctgtatg ttctggccaa tctaggaaca ttttaaaaat acctttgatt
    10201 aaatacagat ttaatattag ttgaattgaa gataaaattt cttaaatttt gtgtaaacac
    10261 cgtaatttta atcttataaa tgaatagcta ccgattttgt agacacttta taattattct
    10321 aaaattaact taatgcacgt tatacaaaat taaaaaatgg agcctaaagc tccatttttt
    10381 aaattgctaa gcgcaaattc agtgctttta caactttaat caccgtatca aaactagggt
    10441 taacatcacc agaaagtgct ttgtaaagac tttctcggcc tagacctgta tctcgtgata
    10501 attgagccat tcctttagct ttagcaatgt tacctaaagc tttagcaatg aatgc
//
