LOCUS       NZ_CP135156            11264 bp    DNA     circular CON 01-FEB-2024
DEFINITION  Acinetobacter pittii strain AR3676 plasmid pAR3676_5, complete
            sequence.
ACCESSION   NZ_CP135156
VERSION     NZ_CP135156.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37485474
            Assembly: GCF_036320505.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter pittii
  ORGANISM  Acinetobacter pittii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 11264)
  AUTHORS   Hua,X. and Yu,Y.
  TITLE     Genetic Characterization of Plasmid-Borne blaOXA-58 and blaOXA-72 in
            Acinetobacter pittii in China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11264)
  AUTHORS   Hua,X. and Yu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2023) Sir Run Run Shaw Hospital, Zhejiang
            University, Qingchundong 3 RD, Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/31/2024 23:07:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,919
            CDSs (total)                      :: 3,824
            Genes (coding)                    :: 3,678
            CDSs (with protein)               :: 3,678
            Genes (RNA)                       :: 95
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 146
            CDSs (without protein)            :: 146
            Pseudo Genes (ambiguous residues) :: 0 of 146
            Pseudo Genes (frameshifted)       :: 51 of 146
            Pseudo Genes (incomplete)         :: 93 of 146
            Pseudo Genes (internal stop)      :: 23 of 146
            Pseudo Genes (multiple problems)  :: 18 of 146
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP135156.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11264
                     /organism="Acinetobacter pittii"
                     /mol_type="genomic DNA"
                     /strain="AR3676"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:48296"
                     /plasmid="pAR3676_5"
                     /geo_loc_name="China: Shaaxi"
                     /lat_lon="33.04 N 107.01 E"
                     /collection_date="2022"
                     /collected_by="Sir Run Run Shaw Hospital"
     gene            join(11053..11264,1..448)
                     /locus_tag="RQL83_RS19400"
                     /old_locus_tag="RQL83_19460"
     CDS             join(11053..11264,1..448)
                     /locus_tag="RQL83_RS19400"
                     /old_locus_tag="RQL83_19460"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="WP_233728940.1"
                     /translation="MFSTSEILGQLLGLSRTATFKTLKSMEKQDLVRLHHIEYELAQRG
                     KQTVWGLTPTGALLATDLDNFQIDFYEVGRIATSTMAHSLAIQRVKVAGLTKGWTDWVS
                     SRKLKQIAAKDKMKWKQIPDALATSLEGKVIAFEVEKTVKTPKRYEVILSNYAKMFLEQ
                     TVDQVFYIGPENIVKRLERLFSNIDKLVVDGKSYPVHENVLKRIQFLSYEDWNELK"
                     /name="replication-relaxation family protein"
     gene            467..847
                     /locus_tag="RQL83_RS19405"
                     /old_locus_tag="RQL83_19465"
     CDS             467..847
                     /locus_tag="RQL83_RS19405"
                     /old_locus_tag="RQL83_19465"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_233728943.1"
                     /translation="MFKDIYDLHTEKREFISLIPNIRNVYLQYNFKVDQNDVRLIFVET
                     DLKGQKLTSVKTEERAKIFQWVLDKHKIDADLRGVKVSLVVNEGFGKPYGYRWDNGNFS
                     VNADIIKLDHEQCETILKQLEA"
                     /name="hypothetical protein"
     gene            complement(1046..1843)
                     /locus_tag="RQL83_RS19410"
                     /old_locus_tag="RQL83_19470"
     CDS             complement(1046..1843)
                     /locus_tag="RQL83_RS19410"
                     /old_locus_tag="RQL83_19470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005009958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_233728945.1"
                     /translation="MSKTLKKYWQSYGGFKALMKSPFLWLSFLMTIISYEFFLDEKWLD
                     IPISMLPSLLGFNLASYSVWLAFGNHKLNKILSKLKEGQLHSRYMEVNSSFIHFILAQI
                     LCLILVVLLKTNSLNNIIYLPNLACFNFYMKFLEYGQIFINGFIFFLFCYCLFSMLAAV
                     LGFIGIASGLDIIYRQESKEEIQTKRLNQQNIIQKEKEKELLNQKIKEELELLKVQKIK
                     EELELSILQKAKNELELLIAQKNSSIKPTQEKIKNIIGFFKSK"
                     /name="hypothetical protein"
     gene            complement(1845..2765)
                     /locus_tag="RQL83_RS19415"
                     /old_locus_tag="RQL83_19475"
     CDS             complement(1845..2765)
                     /locus_tag="RQL83_RS19415"
                     /old_locus_tag="RQL83_19475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005009956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4747 family protein"
                     /protein_id="WP_233728949.1"
                     /translation="MGVIKESKAKTYTFVAFNIRLHPHDKKGIYIDFFNFLQKSQLFSH
                     TGYKYNVIGVDQQKFEPYDDRQNIFLGRFIRYTNIYDAKWYNTEKDEVELKEDLKDLIQ
                     VPNNLKLDGTIFDFMFLLDPHILIVQVDNDETATTANTIGKYFKKVFKNEDFIKKFKDG
                     EITPICQSKDVKILLENPRLKFIEYIIRRPNADLFADLIEAEVLADMEESNSEEYRFQM
                     EAQNGKFLNLSDRVKEIGEISADNGEINALEVPTDSSRYKKISTKGIPLQEPITLPTTL
                     TAKEKWKSILLKLKPKVKKLSQINP"
                     /name="DUF4747 family protein"
     gene            complement(3003..3317)
                     /locus_tag="RQL83_RS19420"
                     /old_locus_tag="RQL83_19480"
     CDS             complement(3003..3317)
                     /locus_tag="RQL83_RS19420"
                     /old_locus_tag="RQL83_19480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_233728951.1"
                     /translation="MSMVRYTRKELNENFSDKQDAEIKRLLAKETVPDEQLDLSDIPEI
                     TDWSHAVRHGQFYRPVKQQTSVRLDADVLAWLKAQGKGYQTRMNKILREAMLEDLKNHS
                     "
                     /name="BrnA antitoxin family protein"
     gene            complement(3304..3591)
                     /locus_tag="RQL83_RS19425"
                     /old_locus_tag="RQL83_19485"
     CDS             complement(3304..3591)
                     /locus_tag="RQL83_RS19425"
                     /old_locus_tag="RQL83_19485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_233728954.1"
                     /translation="MELYFEWDEAKNQKNQKKHDVSFETASLIFEDPLRISIQDRHTNG
                     EERWQTIGMVKGVLMLLVAHTIFDEDDCEIIRIISARQVTKAERNQYEHG"
                     /name="BrnT family toxin"
     gene            complement(3908..4792)
                     /locus_tag="RQL83_RS19430"
                     /old_locus_tag="RQL83_19490"
     CDS             complement(3908..4792)
                     /locus_tag="RQL83_RS19430"
                     /old_locus_tag="RQL83_19490"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_329777049.1"
                     /translation="MLKKPSERQKIQEILDKIEDDSFTPSDIDLILIKLREYSKPKSLF
                     QEISHFAAHNEIRDQGNTFYHMNGFFSSMLFHTKHSFDGKEPLDFSKPIPGYVIEKIKF
                     NIYLSKDTFTEKYKCSLTQFESKFNNVFQKIKGTNTYKLKGTLKGTVRKVTEDMLQLLI
                     SQPLFTQEQIIEEFINVLKENQFSIDENLYQLRCEKIILFIIFLMHGTEYSITEEIKSI
                     SFIDINQEDDLRILSLNAHVPTPYKDTLITCVYPLISTKLDYLYHCSEKIIQSGINEKN
                     RDMLIIKNRKLDF"
                     /name="hypothetical protein"
     gene            complement(4902..5207)
                     /locus_tag="RQL83_RS19435"
                     /old_locus_tag="RQL83_19495"
     CDS             complement(4902..5207)
                     /locus_tag="RQL83_RS19435"
                     /old_locus_tag="RQL83_19495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005020909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_005020909.1"
                     /translation="MNRSNDIVSRDAERDIGAELLMAVDEMLANAPVKTYTITQTDVAL
                     ARQKSELSQREFAETLGVSVRTLESWEQGVRKPSKAAQSLIKLFIKDPSFVREALS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(5191..5511)
                     /locus_tag="RQL83_RS19440"
                     /old_locus_tag="RQL83_19500"
     CDS             complement(5191..5511)
                     /locus_tag="RQL83_RS19440"
                     /old_locus_tag="RQL83_19500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005166860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005020911.1"
                     /translation="MYTICETSLFSKFYSDYWTEKEYNEFKIFLASNPEVGDVEPNSGG
                     IRKVRWGSGKTGKSGGVRVIYYNRLANGQIWLLTIYGKSAVDQLDKKVLRALVEKLNES
                     LK"
                     /name="hypothetical protein"
     gene            complement(5887..6114)
                     /locus_tag="RQL83_RS19445"
                     /old_locus_tag="RQL83_19505"
     CDS             complement(5887..6114)
                     /locus_tag="RQL83_RS19445"
                     /old_locus_tag="RQL83_19505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_077164723.1"
                     /translation="MHSFSVKELDQLIENDLKANKKSSKLLIGYKLFHDLMNDPKFHAE
                     VTNSALSATKRKYKSLKIKITTDEYQLHFE"
                     /name="hypothetical protein"
     gene            complement(6434..6985)
                     /locus_tag="RQL83_RS19450"
                     /old_locus_tag="RQL83_19510"
     CDS             complement(6434..6985)
                     /locus_tag="RQL83_RS19450"
                     /old_locus_tag="RQL83_19510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005108696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_063454750.1"
                     /translation="MPKFTVAELAKKYGIARTSIYDRMKNGSISYEIDAKNRKVIDFSE
                     MQRVYGTPNSQADSKPDSKETNNTTVELYKQLIEELRHDKSIAEERERRMYKEIEQLKD
                     KIDNLTLALGYTPKDIQADSKPDSRNEQIPTAKDLQEIVHLEQSKTVEVEPIQPTVVTT
                     SEPKKRGLLGRLARAVFKEE"
                     /name="plasmid replication DNA-binding protein"
     gene            complement(7068..8042)
                     /locus_tag="RQL83_RS19455"
                     /old_locus_tag="RQL83_19515"
     CDS             complement(7068..8042)
                     /locus_tag="RQL83_RS19455"
                     /old_locus_tag="RQL83_19515"
                     /inference="COORDINATES: protein motif:HMM:NF013235.4"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_233728936.1"
                     /translation="MLKNNLIVKSNQVIEASYTLTTIEQRLILSAIAQVPKGEEISDDV
                     LYPLTTKNLVKLGGDEKASHKEFKDAVNRLYERSIVLRDGDESDSFRWIQEKVFNGSKV
                     AFIRFSKPILPFLSNLKAEFTKYLESDIVGMSSPYSIRFYELIMQYRSVGRREISLEDL
                     RWMFQLQNKYPVWADLKKRVIDQATKEINEHSPYNLTIEPKRTGRKITSIVLKFQDKKT
                     LKTPKDSPNNQLFETQRNSDFVSSNIQTFSDALKQPEKAKESDLGHRASRITGLIMSNG
                     LADRFKQGDESIMGMMKRIKEEITTHTAANQWENKLKEFGVVF"
                     /name="replication initiation protein"
     gene            9230..10954
                     /locus_tag="RQL83_RS19460"
                     /old_locus_tag="RQL83_19520"
     CDS             9230..10954
                     /locus_tag="RQL83_RS19460"
                     /old_locus_tag="RQL83_19520"
                     /inference="COORDINATES: protein motif:HMM:NF014581.4"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA transfer
                     family protein"
                     /protein_id="WP_233728938.1"
                     /translation="MIKFLIWGSAILLSGSLFLEIYKNFILEEISYRLLDACIVLGAIS
                     GFALFYIFSEGLKVKEKRSHIKFKASLGIFFIITVVQGLNTVFLKEYFVYLSIVHIQFF
                     KSAGLGFVGAIIFQRLWILGDQIFTSRNTAKSVLERDSKSDIRDLEKFSSQIKKDYDPK
                     KYFRKNGFFLALNEKDKPIYWLEKTLPHIQVCGMTGSGKGVFLSSIVAQCAKKGEVIVI
                     NDPKNDEFLPHAAYRASIDANTSYQYIDLRKSSYQFNLFEDAGPEEIEELLLAGFELGD
                     KGEASDFYKLADREMAHFVAQNLVKGQTAENFYTEHHEFLTKQAAGFAGKFRELADIKA
                     VNAANGLSFKEIIKSGGVVYIVGSMRNAKIIRIQKMLLVRLIQLAEARDRISGELKPIC
                     VVLDELKYHLSRTALEALGAARDKGLHMVMAHQSLADLKDCPKDLEPDAVIGAVFENTK
                     IKLVYRVEMPETAEIFAEKSGDIGIDVETRLIERSLSLAESVSSERRVSQTETNLIEKN
                     IFLTLPKRCGVLFGVGLPKLVHTSHYQAEKDINAVKTNDQKRDNEEIDLNEIDFGTL"
                     /name="type IV secretory system conjugative DNA transfer
                     family protein"
ORIGIN
        1 tgaagtaggg cgaatagcaa catcgactat ggctcatagc cttgctattc aacgtgtcaa
       61 agtagcaggg cttacaaaag gttggactga ttgggttagt agtcgaaagc ttaagcaaat
      121 agctgctaag gacaaaatga aatggaaaca gatcccagat gcactcgcta cctctttaga
      181 aggtaaagtt attgcctttg aagtagaaaa aacggttaaa acaccgaaga ggtacgaagt
      241 aattctcagt aattacgcca agatgttctt agagcagacc gtagaccaag ttttttatat
      301 tggtcctgaa aatattgtaa aacgactaga acgtctattt tcaaatatag ataagcttgt
      361 agttgatggg aaaagctatc cggtacatga aaacgtactt aaacgtattc aatttttgtc
      421 ttatgaagac tggaacgaat taaaataaac agtataggat caaccaatgt tcaaagatat
      481 atatgatttg catacagaaa aaagagagtt tatttctcta attccaaata taagaaatgt
      541 ttatcttcaa tataatttta aagtcgatca gaacgatgta aggctaatct ttgttgaaac
      601 agacctaaaa ggtcaaaaac taacaagtgt aaaaactgaa gagagagcaa aaatttttca
      661 atgggtattg gataaacata aaatagatgc tgatttaagg ggagtaaaag tttcccttgt
      721 agtgaatgag ggcttcggta agccatatgg ctacagatgg gataacggta atttttctgt
      781 aaatgcagac ataataaaat tagatcacga gcaatgtgag actattctaa agcagttaga
      841 agcttgaaaa ataaaatcag aaaacctaga tattatgtct aggctttttt atttcatata
      901 agaggtttta tgttaagcag ccatcttatc taactaaaag ttgtcgagaa aatcggtgtc
      961 tgtttattca acgttcacca gcagcctagg gggcttcccc gaaggctatg ggaacttgag
     1021 agagttgatt acatttagta agaaattatt tagatttgaa gaatccaata atatttttaa
     1081 ttttctcttg agttggtttg atagaactat ttttttgagc tatgagtaac tctaattcat
     1141 ttttagcttt ttgaagtatt gataattcta gttcttcctt aattttttga actttcaata
     1201 attctagttc ttctttaatt ttttgattaa gtagttcttt ttctttctct ttttgaatga
     1261 tgttttgttg atttaatctt ttagtttgaa tttcttcctt agattcttgt ctataaataa
     1321 tatctaatcc tgatgctatt ccaataaatc ctaaaacagc agctaacatg ctaaataagc
     1381 agtagcaaaa taagaaaaat atgaaaccat ttataaaaat ttgcccatat tctaggaatt
     1441 tcatatagaa attgaaacat gctaaatttg gtaaataaat aatattattt agagagtttg
     1501 tctttaatag aactacaaga attaagcata atatttgagc taaaatgaaa tgaataaatg
     1561 aagaatttac ctccatatac cttgaatgca attgaccttc tttaagtttt gaaaggattt
     1621 tatttaattt atgatttcca aaagctagcc atactgaata actggcaagg ttaaacccta
     1681 ataaactagg taacatactt ataggaatat ctaaccactt ttcatctaaa aaaaactcat
     1741 aagatattat agtcatcaaa aatgataacc ataaaaatgg tgatttcatc agagccttga
     1801 aaccaccata actttgccaa tattttttta aagtctttga catattatgg atttatctgt
     1861 gaaagttttt taactttggg ctttagctta agtaaaatac ttttccattt ctctttagct
     1921 gtaagagtag taggtagtgt aataggttct tgaagaggaa taccttttgt actaatcttt
     1981 ttatatctac tagagtcagt tggtacttct aaagcattta tttctccatt gtcagcagat
     2041 atttctccta tttccttaac tctatcacta agattcagga actttccatt ttgcgcttcc
     2101 atctgaaatc tatattcttc agaattactt tcttccatat ctgctaatac ttcagcttca
     2161 attaaatctg caaatagatc agcatttggt cgtctaataa tgtattcaat aaattttaaa
     2221 cgcggatttt ctaaaagaat ctttacatct ttagattgac agataggtgt tatttctcca
     2281 tctttaaatt tctttataaa atcttcattt ttaaatactt ttttaaaata ttttccgatg
     2341 gtgttagctg ttgtagctgt ttcgtcatta tcaacttgaa cgattaaaat gtgagggtct
     2401 agaaggaaca taaaatcgaa aatagtaccg tctaacttta agttattagg aacttgaatt
     2461 aaatctttta aatcttcttt gagttctact tcatcttttt cagtgttata ccatttagca
     2521 tcataaatat tggtatagcg aataaaacga cctaaaaaaa tattctgtct atcatcatat
     2581 ggttcaaatt tttgttgatc tacgccaata acattgtatt tataaccagt atgtgaaaat
     2641 aactgtgatt tttgtaaaaa attaaagaaa tctatataaa ttcctttttt atcatgggga
     2701 tgaaggcgaa tattgaatgc aacgaaagta tatgtttttg cttttgattc cttaataacg
     2761 cccatgaata ccccaaaata ttattaaaat tcagataaat tataataaca caatgtattt
     2821 caatgatatg gagcatatta agatagtttt tttgatgagt ctaaagtcat tgtcgttaga
     2881 gtgattaaat tttttagatg catctcttta cgcttaattt tgtagatatt ttatattttt
     2941 cctgaattta acataattca cctcatgtaa aattgaaaat gggagcctat agctcccatt
     3001 ttttatgaat gattttttag atcttctagc attgcttcac gcagaatttt attcatacgt
     3061 gtctgatagc ctttaccttg tgcttttaac cacgcaagta catctgcatc aagacgaaca
     3121 gaggtttgtt gcttcactgg acgataaaat tgcccatggc gaacagcatg actccagtcg
     3181 gtaatctcag gaatgtcaga cagatctaat tgctcatctg gtacagtttc cttcgcaagc
     3241 aagcgtttaa tttcagcatc ttgcttgtca ctgaaatttt cattcagttc tttgcgtgta
     3301 tatctaacca tgctcatatt gattacgctc cgctttggtg acttgccttg cactaatgat
     3361 tcgtatgatt tcacagtcat cttcatcaaa gatggtgtga gcaaccaata acatcagtac
     3421 gcccttgacc ataccaatgg tttgccaacg ttcctcccca ttggtatgcc gatcttggat
     3481 tgagatcctt aacggatctt caaaaatgag gcttgctgtt tcaaaagata catcatgttt
     3541 tttctgattc ttttggttct ttgcctcatc ccattcgaaa tacagttcca taaaaaacat
     3601 tcaaatttgt atatacaata atgtatcata aaattgtatc catcaacccg attggtgtaa
     3661 tttttagtca aaattattgg caaaacattc caaaaccgct cataaaaaag ccgacttgtt
     3721 gcaaagtcgg ctttttgagc tttttgtgat cagggtgtaa tttttaagta attgatattt
     3781 aaattaatat acaagtgtac ttcgtataat cgccattatg ttaaatggaa ggtaaattgg
     3841 tggaaaagtc cagttatttt ggtgttgtct acgagtagat tattaatgga tctcacctaa
     3901 tagttaacta aaaatctaat tttctatttt taattattaa catatctcta tttttctcat
     3961 taattcctga ttgaatgatt ttctcagaac aatgatacaa atagtctagc tttgttgaga
     4021 ttagaggata tacacacgtt atgagagtat ctttatatgg agttggtaca tgagcattga
     4081 ggctcaatat tcttaaatca tcttcttgat ttatatcaat aaaagatata gattttattt
     4141 cttctgtaat actatattcg gtaccatgca ttaaaaaaat tataaataga ataatttttt
     4201 cacaacgtaa ctgatataaa ttttcatcaa tggaaaactg attttctttt aaaacgttga
     4261 tgaattcttc aattatctgt tcttgtgtaa agagtggttg gctaatcaaa agttgcaaca
     4321 tatcttctgt tacttttcga actgtacctt taagagttcc ttttaattta taggtatttg
     4381 ttcccttgat cttctgaaat acattattaa atttactttc aaattgagtt agagagcatt
     4441 tgtacttttc tgtaaaagta tccttagata agtaaatgtt aaactttatt ttttcaataa
     4501 catagcctgg tattggttta ctaaaatcta agggttcttt tccatcaaag ctatgcttcg
     4561 tatgaaataa catactgctg aagaaaccat tcatatgata gaaagtattg ccttgatctc
     4621 ttatttcatt atgtgcggca aaatgtgaaa tttcttgaaa taaactttta ggcttagaat
     4681 attctcttag ttttattaaa attaaatcta tatctgatgg agtaaatgag tcatcctcta
     4741 ttttatccaa tatctcttgt attttttgtc tttcggaagg cttctttaac atcttgctca
     4801 ttcaatttga tctttagtaa tactattaaa attaacataa tacacgttat gcgaagtcaa
     4861 aaatgggagc ctttagctcc catttttact atatttttta attagctaag agcttctcta
     4921 acaaaagaag gatctttgat aaacagctta attaaggatt gtgctgcttt agatggttta
     4981 cgtacacctt gttcccaact ttccaaagta cgcacagata cacctaaagt ttcggcaaat
     5041 tcacgttggc ttaactctga tttttggcga gctaaagcga catcagtctg agtaatggta
     5101 taagttttta ctggagcatt ggcaagcatt tcatctactg ccataagtaa ttcagcacca
     5161 atatctcgtt cagcatcacg gcttacaata tcatttgagc gattcattta acttctccac
     5221 taaagcacgt agtacttttt tatcgagttg atcaacagca gatttcccat aaatggtaag
     5281 taaccatatt tgtccattag ctagacggtt gtaatagatt actctgactc caccgctttt
     5341 acctgtctta ccagatcccc aacgaacttt acgtattcct cctgagttag gttctacatc
     5401 accaacttca ggattggaag caagaaaaat tttaaactcg ttgtattctt tttctgtcca
     5461 atagtctgaa taaaacttac tgaacaaaga tgtttcgcat attgtataca tcaagcttcc
     5521 atactatcta ctactatgta gtaattttat tgtactacat agtagtactt tatcaatgtt
     5581 ttttctaaaa tttcgtataa cagccattat gttaaatgga tgatagatct gaataattgt
     5641 tacttataag acatgctaaa attagatcaa aatctattac ctataggtca agttatggaa
     5701 acaattatta tttcattgat cttggttatt attctgattt tactgaccat aatgtataac
     5761 aaaaaaaagt gaaacatacg cttactcaag agcttaaacg tcctgaactg atagctgaaa
     5821 tcaataaaat ttcacacatt gattaatgta aaaaaagagc atattgctct tttttttttg
     5881 ggggggttat tcaaagtgaa gttgatattc atctgtagtt atttttatct ttagactttt
     5941 atattttcgt ttggtagcac taagagctga attggtgact tctgcatgaa atttgggatc
     6001 attcatcaga tcgtgaaata gtttatatcc aatcaataac ttacttgatt ttttatttgc
     6061 ttttaaatca ttttcaatta attgatctaa ttcttttaca gaaaaactat gcatcttaac
     6121 ctcactagta tgtttctgag ggagaaccat acaggtttca taataggaac taaagacatt
     6181 atttggcttc tgctatatgg aatattactg taaaagtaat aggttaaaat tatatcacgt
     6241 ttagattttc taaaattaac ataatacacc ttatacgaaa tgcgttataa ttatctatag
     6301 aaatcaatgc tgtagtttct tttttgccca acctccccca aggtgggctt tttttttatg
     6361 atttttcacg ttccacgctc aataagtgag caatgttcca cgattttgtg tagccccaat
     6421 gaacaatcaa atcctactcc tccttaaaaa ctgcccgagc taaacgaccc aataagcccc
     6481 tttttttagg ttctgatgta gttacaacag ttggctgaat tggttcgact tcgacagttt
     6541 tactttgttc aagatgcact atttcttgta agtctttagc tgtagggatt tgttcattcc
     6601 gactgtcagg cttactatca gcctgaatat cttttggtgt atacccaagg gcgagagtta
     6661 aattatcgat cttatcttta agctgctcaa tctctttgta catacgcctt tcacgttctt
     6721 ctgcgataga tttatcgtgc cgtaattctt caatcaattg cttgtacagt tcaactgtag
     6781 tgttgttagt ttctttacta tcaggcttac tgtcagcctg actgttaggc gtaccgtaaa
     6841 cacgttgcat ttcactaaaa tcaattactt ttctattttt ggcatctatt tcgtaactga
     6901 tagaaccatt cttcatacga tcataaattg atgtacgagc aattccatat tttttagcta
     6961 attctgcaac tgtaaattta ggcattatta aactcttcaa attgttgtag atctcaaagt
     7021 atttctacca atgctaataa ttgtgtcttg agattatttg gtcatgacta gaaaaccacg
     7081 ccaaactctt tcaatttgtt ttcccattga tttgctgctg tatgagttgt gatttcttct
     7141 ttgatacgtt tcatcatgcc cataattgat tcatcacctt gcttaaatcg atctgctaaa
     7201 ccattcgaca tgattagccc tgtaattcta cttgctctat gccctaaatc gctttcttta
     7261 gctttttcag gctgtttcag agcatcgcta aaggtttgga tattagaaga gacaaaatca
     7321 ctatttcttt gagtttcaaa aagttgatta ttgggcgaat cttttggtgt ttttaaggtt
     7381 tttttgtctt gaaatttaag aacaatactt gtgatcttac gtcctgtccg ttttggttcg
     7441 atagtaaggt tgtaagggct atgctcatta atttctttgg ttgcctgatc aataactcgt
     7501 ttttttaaat ctgcccaaac tggatattta ttttgtagct gaaacatcca acgaagatct
     7561 tcaaggctaa tttcacgtcg gccaacacta cggtattgca ttattagttc atagaaacga
     7621 atagagtaag gactagacat accaacaatg tcagattcta agtatttagt gaactcagct
     7681 ttcaaattag ataaaaatgg taatataggc tttgagaagc gaataaaagc tactttagat
     7741 ccattaaata ctttctcttg aatccatcta aatgagtccg actcatcacc atcccgaagt
     7801 acgatagatc gctcatataa acggtttacg gcatctttaa attctttgtg tgatgctttt
     7861 tcatctccac cgagtttgac caaatttttg gtagtaaggg gatataaaac atcgtctgat
     7921 atttcctcac cttttggaac ttgagcaatg gcacttagaa ttaatcgttg ttctattgtt
     7981 gtaagagtat aggacgcttc aatgacctga tttgatttta cgatcaaatt atttttaagc
     8041 atattgatag taactttaaa ttattatgga caaatatata gctgtcattc ttttgtgtca
     8101 acagtgcggg aatctgtcca tattagtgcg ggaatctgtc catattagtg cgggaatctg
     8161 tccatattta gcgggaatct gtccatactt aaggctgaaa agccttgata tacaatggtt
     8221 tcaagaggct taaaatcttt taaaagtatt aaaataataa aaaaggaaca acatgaaaaa
     8281 ctgcccttgg ttttcgctac gctcaaactc tattgaatct cgctttcgct cgattcgtcg
     8341 gggcaatttt ttggtttatg tttccgtaaa tttgagaaaa agcaaaagca actcggaatt
     8401 cgcttcgctc atagtacttt tttctcgcta cgctcggtct agaagcaaat ggatttaact
     8461 aatcattcta tttttaaaaa ttttgatact taaaagcatg ttttccagaa ggtctaggcg
     8521 cgacttttct ttgtgtcgct cgtagacact cgctaatcat ttaatcttag gatttaacaa
     8581 tacagagaat tgaaatagag aacataaata gaaacaaagt gttctcttat tatgttctcc
     8641 atgaggttgt gtatcctcgg ataacgccac cgctacgcta cacttttaaa attatgataa
     8701 agccataatt ttaaaccact ttcgtggacg tgttggctaa aatcgctgaa cgcgattttt
     8761 atcctctacg gaagccccgt ctgcgcgttt cgcgcgcgac ttcccacccc tctacttgat
     8821 gatgagcttc attggctacg caatgaactc accgatcaag cttaacgtgg cgcgggaagt
     8881 cgcgcgttcg ttcctctcgc ttgacggggg ggcttgcccc cacttgggtc gttcgttggg
     8941 actcacttaa aaactcggca agctcgtttt tatccctgcg tggctccccc aagtcctctc
     9001 cgctaggctc cgaggatact cactttcgtt cgtacccctc cggggcttaa cgaccgcttg
     9061 gagcggtcgg tcacgtatcc aatttataga ataataataa attgctaatg gagtatatta
     9121 aatttgcggt atatgttact gatttaaaac actttttatt tatatttttg ttgaaaaagt
     9181 tttgttaatc ctttctaatt gatagaaaag aggcagacta gtaaaaaata tgattaaatt
     9241 tctaatttgg ggttcagcaa tcctcttatc gggttctttg tttttagaaa tatataaaaa
     9301 ttttatttta gaagaaatct cttatcgctt acttgatgcc tgtattgttc taggagcgat
     9361 ttcaggtttt gcattatttt atattttttc agaaggtctt aaagttaagg aaaaaagatc
     9421 acatattaag tttaaagcct cccttgggat attttttatt attacagtag tacagggttt
     9481 aaatactgtt tttctcaaag aatactttgt ttatttatca atcgttcata tccaattttt
     9541 taaatctgca ggcttaggct ttgtcggagc tatcattttc caacgtctgt ggattttggg
     9601 agatcaaatt tttacttcaa gaaatacagc aaaaagtgtt ttagaacggg atagcaaaag
     9661 cgatatccgt gatttagaga agttttcgag tcaaatcaag aaagattacg atcctaaaaa
     9721 atattttaga aaaaatggat tttttctagc tttaaatgaa aaagataaac cgatctattg
     9781 gcttgaaaag acacttcctc atatccaggt ttgcggtatg accggaagtg gaaaaggagt
     9841 tttcttaagt tctatcgttg ctcaatgtgc aaaaaaagga gaagtaattg ttattaatga
     9901 tccgaaaaat gatgaatttt tgccacatgc tgcatatcgt gcatctatag atgcaaacac
     9961 cagttaccaa tatatcgatc taagaaaatc aagttatcaa tttaatcttt ttgaagatgc
    10021 aggcccagaa gaaatagaag aattactctt agcaggcttt gaacttggag ataaagggga
    10081 agcctcagat ttttataaac ttgcagatag agaaatggct cattttgttg cacaaaatct
    10141 ggttaaaggg caaacagcag aaaactttta tacagaacat catgaatttc taactaaaca
    10201 ggctgctgga tttgcaggta agtttcgaga attggctgat attaaagctg tgaatgctgc
    10261 caatggtctg tcctttaaag aaatcattaa atcaggggga gtagtttaca ttgttggttc
    10321 aatgcgtaat gcaaagataa ttcgtattca gaaaatgcta ctggttcgtc tcatacagtt
    10381 ggcagaagct agggatagaa tatcaggaga gctaaagccg atttgtgtgg ttttagatga
    10441 gctgaaatat catctttccc gaactgcgct agaagcttta ggagcagcgc gcgacaaagg
    10501 tctacatatg gtcatggcac atcaaagcct tgctgatctt aaggattgcc cgaaagatct
    10561 tgaacccgat gcagtcattg gagctgtgtt tgaaaacact aagattaaat tggtttatcg
    10621 tgtggaaatg ccggaaactg cagaaatttt tgcagaaaaa agtggagaca tcggaatcga
    10681 tgtagaaact cggttgattg aacgttcttt aagtcttgca gaatcagtca gtagcgaacg
    10741 tagggttagt caaactgaaa ctaatttaat cgagaaaaat atctttttaa ctttgcctaa
    10801 acgatgtggc gttttatttg gagttggact cccaaaactc gtgcatacaa gtcactatca
    10861 ggctgagaaa gatataaatg cagttaaaac caatgatcag aaacgagata atgaagaaat
    10921 tgatttaaat gaaattgatt ttgggacgtt ataaagcatg ggagattcat ctttttttat
    10981 tcacgataaa gctaaacaat cacagcggat tgaagaaaaa aaaacattgc cttaggtttt
    11041 ttagctaatg aaatgtttag tacttctgaa attttaggac aattacttgg attaagtcgt
    11101 acagccacgt ttaaaacgct caaatcaatg gaaaagcaag atctggttag gttgcaccat
    11161 attgaatatg aactcgcaca gcgaggcaaa cagacggtct gggggcttac ccccacagga
    11221 gcattgttgg ctactgatct agataatttt caaatcgatt ttta
//
