LOCUS       NZ_CP135155            19700 bp    DNA     circular CON 01-FEB-2024
DEFINITION  Acinetobacter pittii strain AR3676 plasmid pAR3676_4, complete
            sequence.
ACCESSION   NZ_CP135155
VERSION     NZ_CP135155.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37485474
            Assembly: GCF_036320505.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter pittii
  ORGANISM  Acinetobacter pittii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 19700)
  AUTHORS   Hua,X. and Yu,Y.
  TITLE     Genetic Characterization of Plasmid-Borne blaOXA-58 and blaOXA-72 in
            Acinetobacter pittii in China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 19700)
  AUTHORS   Hua,X. and Yu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2023) Sir Run Run Shaw Hospital, Zhejiang
            University, Qingchundong 3 RD, Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/31/2024 23:07:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,919
            CDSs (total)                      :: 3,824
            Genes (coding)                    :: 3,678
            CDSs (with protein)               :: 3,678
            Genes (RNA)                       :: 95
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 146
            CDSs (without protein)            :: 146
            Pseudo Genes (ambiguous residues) :: 0 of 146
            Pseudo Genes (frameshifted)       :: 51 of 146
            Pseudo Genes (incomplete)         :: 93 of 146
            Pseudo Genes (internal stop)      :: 23 of 146
            Pseudo Genes (multiple problems)  :: 18 of 146
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP135155.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..19700
                     /organism="Acinetobacter pittii"
                     /mol_type="genomic DNA"
                     /strain="AR3676"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:48296"
                     /plasmid="pAR3676_4"
                     /geo_loc_name="China: Shaaxi"
                     /lat_lon="33.04 N 107.01 E"
                     /collection_date="2022"
                     /collected_by="Sir Run Run Shaw Hospital"
     gene            356..1360
                     /locus_tag="RQL83_RS19295"
                     /old_locus_tag="RQL83_19355"
     CDS             356..1360
                     /locus_tag="RQL83_RS19295"
                     /old_locus_tag="RQL83_19355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786738.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA]; GO:0008324 - monoatomic cation
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_process="GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="WP_023187986.1"
                     /translation="MSNQRDHNHKCHDHSHEGHDHSHEGHDHSHVPDVSNGNERKILIS
                     FFIIFGFMLVEATGGIISGSLALLADAGHMLTDAVALGLAYAAFRFGRRIADNSKTFGY
                     MRFEVIAGLMNSLTLFAIVGWIIYEAIERFQSPQPVMAGPMFIVAIIGMLVNLFVLWYL
                     TRGDTEHVNVKGAILHVMGDLLGSVGAIIAAIVIWYTGWTPIDPILSVLVSLLVLRSAW
                     SLFKRTLHILLEGAPENANAQEITEYLLKNVSGIEKITHIHIWMITSGRSMATLHVKPK
                     DGVDLATLTLNIENELKSKFSIEHPTIAIDWQGNSTCSLENPTQIDPHAGHNH"
                     /name="cation diffusion facilitator family transporter"
     gene            1401..3089
                     /locus_tag="RQL83_RS19300"
                     /old_locus_tag="RQL83_19360"
     CDS             1401..3089
                     /locus_tag="RQL83_RS19300"
                     /old_locus_tag="RQL83_19360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009586881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023187987.1"
                     /translation="MSDKYYNLGDYQRQITTSSPDTQLWFDRGLLWTYCFNPSEAINCF
                     KKALEHDAKCAMAYWGIAFASGPNYNKAWHLFDPSDLQNTIPYINEIMEQAKLAATHNA
                     TQFEKDLIDALAPRFPKHVTIDEQDFHELNFAYAQAMRSFYEKYSNDLDAAAMFADALM
                     CLSPRKLWDLDTGEAIGLGTVEARNVLEVAFTKPGANRHPVINHLYIHLMEMSPYPEVA
                     LPAADNLRNLSPDGSHLAHMSTHIDNACGDWRRVVYSNLDAIAADERYFSREKNPGGWN
                     ILYRVHNLMVGAYGAMMAGKSQEALFMAHRINDILTPEVLHITTPPMANLTESHCTTLP
                     HVMIRFGMWEDILKLELPKDKGLYSSTTAMIHYAKGIAFASLRRVKEAEKERESFIKAV
                     ANVSEERFNSIPVKEVEALKIAAKMIHGEIEYRKGNTKEAFAILREGIKLEDALPYCDP
                     PAWLQPVRHAFCALNMEQGNFEVAAQEYRIELGLEGDLGRRRIRPNNVWSLHGLYECLA
                     KLGLEKEARDISLQRDIALASADINIEASCFCRLSAFEDNTTSCC"
                     /name="hypothetical protein"
     gene            complement(3314..3628)
                     /locus_tag="RQL83_RS19305"
                     /old_locus_tag="RQL83_19365"
     CDS             complement(3314..3628)
                     /locus_tag="RQL83_RS19305"
                     /old_locus_tag="RQL83_19365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008556.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="WP_023187988.1"
                     /translation="MNFNNHPIPLSQDEIMSLAETFRLLGDASRLKILLFCMRGPSSVG
                     EISDALDLSQSLVSHNLRLLRSARLVKGERQSKQVFYEIDDLHVSQVLLDMASHISEEH
                     "
                     /name="metalloregulator ArsR/SmtB family transcription
                     factor"
     gene            3749..6235
                     /locus_tag="RQL83_RS19310"
                     /old_locus_tag="RQL83_19370"
     CDS             3749..6235
                     /locus_tag="RQL83_RS19310"
                     /old_locus_tag="RQL83_19370"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536249.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0019829 - ATPase-coupled monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="WP_023187989.1"
                     /translation="MGKATDKLSNNMKVTLKIEGMTCASCVGRVETALKKVDGVQSASV
                     NLATERADIVLDQPVDRQVLVHAIEQSGYDVPANNIELSIKGMTCASCVGRVEKALKAV
                     AGVKEANVNLATERATVSGSANVDDLITAIDKAGYDATEIQASIPDQTELLEKKDQERA
                     ELKRDLILATLLALPVFILEMGSHLIPGVHQLIDQTIGMQNSWYLQFILTTLVLVIPGR
                     RFYLKGLPALLRLAPDMNSLVAVGTLAAYLFSIVATFIPNILPTGTVNVYYEAAAVIVA
                     LILLGRFLEAKAKGRTSEAIQRLVSLQAKTAHVLRDGQMVDIALDQVVADDIIIVKPGE
                     RIPVDGQVTDGKSFVDESMITGEPIPVEKGIGSDVVGGTINQNGTLTFTAVAVGGETML
                     AQIIRLVEQAQGSKMPIQAVVDKVTLWFVPAVMIAAVLTFLVWLIFGPSPALTFALVNA
                     VAVLIIACPCAMGLATPTSIMVGTGRGAELGVLFRKGEALQLLKDAKVVAVDKTGTLTE
                     GHPVLTDFEVTQLFERDYVLGMVAAVESRSEHPIAKAIVDAAKSENLALPKVDTFDSVT
                     GMGVYATINGNEKINIGADRYMVQLGIDVAPFAVTAQRLGDEGKSPLYVAVDGELAGII
                     AVADPIKSTTPAAIKALHQLGLKVAMVTGDNVRTAQAIAKQLGIDEVIAEVLPEGKVSA
                     VKELKAKYGHIAFVGDGINDAPALAEADVGLAIGTGTDVAIESADVVLMSGNLQGVANA
                     IALSKATIGNIHQNLFWAFAYNTMLIPVAAGILYPAYGILMSPIFAAGAMALSSVFVLG
                     NALRLRRFQSPSVSQG"
                     /name="heavy metal translocating P-type ATPase"
     gene            6246..6704
                     /gene="cueR"
                     /locus_tag="RQL83_RS19315"
                     /old_locus_tag="RQL83_19375"
     CDS             6246..6704
                     /gene="cueR"
                     /locus_tag="RQL83_RS19315"
                     /old_locus_tag="RQL83_19375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016540066.1"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006878 - intracellular copper ion
                     homeostasis [Evidence IEA]; GO:0006355 - regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="WP_061403561.1"
                     /translation="MNIGKVSKASGVTAKMIRYYEQIGLIPAAGRNNAGYRSYSVNDVE
                     RLKFIKRSRELGFSVAEISDLLDLWNNRSRQSADVKRLAQGHIEKLEERINDLQQMANT
                     LKDLIDCCAGDNRPDCPILEGLQEPHNEIKDVHHHNDLLSVSPLTRKK"
                     /name="cueR"
     gene            complement(6917..7105)
                     /locus_tag="RQL83_RS19320"
                     /old_locus_tag="RQL83_19380"
     CDS             complement(6917..7105)
                     /locus_tag="RQL83_RS19320"
                     /old_locus_tag="RQL83_19380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004653564.1"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing protein"
                     /protein_id="WP_000394310.1"
                     /translation="MEFHVEDMTCGGCARGVTRAIQAIDANAKVITEPPNRSVKVETTA
                     TQQQIVEALTEAGFPPR"
                     /name="heavy-metal-associated domain-containing protein"
     gene            complement(7186..7524)
                     /gene="fdx"
                     /locus_tag="RQL83_RS19325"
                     /old_locus_tag="RQL83_19385"
     CDS             complement(7186..7524)
                     /gene="fdx"
                     /locus_tag="RQL83_RS19325"
                     /old_locus_tag="RQL83_19385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019384153.1"
                     /GO_process="GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="WP_001124186.1"
                     /translation="MPKIIVIPHEKICPEGAELEIESGSNLCRSLLDHGIPIEHACDLS
                     GACTTCHVVLKKGFDSLEEMDDVEADLLDRAWGLTAQSRLSCQVKVQDENLEVEIPKYT
                     VNYISEGH"
                     /name="fdx"
     gene            complement(7615..8295)
                     /locus_tag="RQL83_RS19330"
                     /old_locus_tag="RQL83_19390"
     CDS             complement(7615..8295)
                     /locus_tag="RQL83_RS19330"
                     /old_locus_tag="RQL83_19390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="WP_000680279.1"
                     /translation="MKHFVPMLMIGAIVFSAGCSSSDAPKENSMSENKHESHSMHHDMN
                     GSAKTDNPSTNAYMEVNTRMHSGMGIDFTGNADTDFMLGMIPHHQGAVDMAKVVLQYGS
                     DPEVRQLAENVISAQESEIKMMNEWLAKNKNHSGSKNEDSPSTQAYIKINDEMHADMMV
                     PFTGNADVDFMRGMIPHHQGAIDMAKVVLQYGSDPEVRQLAEDVISAQESEIKMMNEWL
                     SKHK"
                     /name="DUF305 domain-containing protein"
     gene            8729..9861
                     /locus_tag="RQL83_RS19335"
                     /old_locus_tag="RQL83_19395"
     CDS             join(8729..8981,8981..9861)
                     /locus_tag="RQL83_RS19335"
                     /old_locus_tag="RQL83_19395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032072965.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISAba14 family transposase"
                     /protein_id="WP_225530522.1"
                     /translation="MARRPRRNHSNDFKAKVALAAIKAEKTLAELSSEFDVHQNQIIDW
                     KNQLISASSQAFDQSKAPSEPPIDLKKLHAKIGEQALEIGFFRRCVEETGPLQPQKLID
                     HSLQISVSKQAKLLKVSRGCYYYRPKPVSSSDLKLMRCIDELHMQYPFAGSRMMRDLLN
                     RQGHHIGRRHTRTLMKKMGIQALYCKPNLSQANQAHRKYPYLLKGLAIQRSNQVWSTDI
                     TYIPMAKGFVYLCAVIDWHSRKVLAHRVSISMEVDFCISALNEAIEKYGSPEILNTDQG
                     SQFTSDAFIDVLKSNGIQISMDGKGRWVDNVMVERLWRSVKYEEVYLKAYSSVTDAKKQ
                     LSAYFEFYNLKRPHSSLDKMTPNEFYYDQLPQQNKVA"
                     /name="IS3-like element ISAba14 family transposase"
     gene            <9925..10704
                     /locus_tag="RQL83_RS19340"
                     /old_locus_tag="RQL83_19400"
                     /pseudo=""
     CDS             <9925..10704
                     /locus_tag="RQL83_RS19340"
                     /old_locus_tag="RQL83_19400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611903.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /name="ISNCY family transposase"
     gene            complement(<10844..11212)
                     /locus_tag="RQL83_RS19345"
                     /old_locus_tag="RQL83_19405"
                     /pseudo=""
     CDS             complement(<10844..11212)
                     /locus_tag="RQL83_RS19345"
                     /old_locus_tag="RQL83_19405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015586042.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element ISAba3 family transposase"
                     /name="IS1-like element ISAba3 family transposase"
     gene            11293..12135
                     /locus_tag="RQL83_RS19350"
                     /old_locus_tag="RQL83_19410"
     CDS             11293..12135
                     /locus_tag="RQL83_RS19350"
                     /old_locus_tag="RQL83_19410"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002002480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbapenem-hydrolyzing class D beta-lactamase
                     OXA-58"
                     /protein_id="WP_002002480.1"
                     /translation="MKLLKILSLVCLSISIGACAEHSMSRAKTSTIPQVNNSIIDQNVQ
                     ALFNEISADAVFVTYDGQNIKKYGTHLDRAKTAYIPASTFKIANALIGLENHKATSTEI
                     FKWDGKPRFFKAWDKDFTLGEAMQASTVPVYQELARRIGPSLMQSELQRIGYGNMQIGT
                     EVDQFWLKGPLTITPIQEVKFVYDLAQGQLPFKPEVQQQVKEMLYVERRGENRLYAKSG
                     WGMAVDPQVGWYVGFVEKADGQVVAFALNMQMKAGDDIALRKQLSLDVLDKLGVFHYL"
                     /name="carbapenem-hydrolyzing class D beta-lactamase
                     OXA-58"
     gene            12226..12932
                     /locus_tag="RQL83_RS19355"
                     /old_locus_tag="RQL83_19415"
                     /pseudo=""
     CDS             12226..12932
                     /locus_tag="RQL83_RS19355"
                     /old_locus_tag="RQL83_19415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015586042.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /name="IS1 family transposase"
     gene            13106..13933
                     /locus_tag="RQL83_RS19360"
                     /old_locus_tag="RQL83_19420"
     CDS             13106..13933
                     /locus_tag="RQL83_RS19360"
                     /old_locus_tag="RQL83_19420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073659.1"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]; GO:0043565 - sequence-specific DNA
                     binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="WP_000073658.1"
                     /translation="MSNWVNVTQDKSTGIELIHAHFKGFAYDPHLHSSYLIGVTELGHQ
                     QFNCRKKIIDSYQGQTFMLEPEEVHDGNAPDPLGFTYKMMHLDPAWLKKSYEGIFNEPI
                     ELAIESTLRSDPQLSHLILSTYNILNNNESQLMKDTYLDLLLENLVNKKILYKREKSIQ
                     TLPNIALKLKEILHESLFHDLDLQTLSTLVKTDRFYINRVFKKNFNCSPHQYLIQLRLN
                     KAKELLRKGLSATTVASYLCFSDQSHLGRWFKRCYGITLNQYQKACTNVLDIS"
                     /name="AraC family transcriptional regulator"
     gene            13983..14588
                     /locus_tag="RQL83_RS19365"
                     /old_locus_tag="RQL83_19425"
     CDS             13983..14588
                     /locus_tag="RQL83_RS19365"
                     /old_locus_tag="RQL83_19425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="WP_000447788.1"
                     /translation="METILAFCAFSFVASITPGPTNFLILSTSHQFSIKKTLGLIFGGS
                     IGAASLVLITGLGIGTTLNEYPKIQLILSFTGGIWLSYIGWKIFNYRPDLESKIQNQNK
                     KNGFFTGFFLQFINPKSWLMAIAVITVYTAQLANYYQSLILFSFIFMLISLPCLFIWAY
                     LGKITKKILSTAHHMVLFNRVLGTVLLISVWYPILHAI"
                     /name="LysE family translocator"
     gene            14692..15012
                     /locus_tag="RQL83_RS19370"
                     /old_locus_tag="RQL83_19430"
     CDS             14692..15012
                     /locus_tag="RQL83_RS19370"
                     /old_locus_tag="RQL83_19430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000897307.1"
                     /translation="MLFIETSIFTKQIKDLVSDEEYRQLQQDLLVQPDRGDLIKNGGGI
                     RKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAYPKSVKDNLTDKETAILHQLVKEQF
                     HG"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            15005..15277
                     /gene="nadS"
                     /locus_tag="RQL83_RS19375"
                     /old_locus_tag="RQL83_19435"
     CDS             15005..15277
                     /gene="nadS"
                     /locus_tag="RQL83_RS19375"
                     /old_locus_tag="RQL83_19435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="WP_000369781.1"
                     /translation="MDNNLFDDLVASIKEAGAIKRKEVKASRVTELELPDIKEVREKTG
                     LSQNEFAARLHISPRTLQNWEQGRRYPTGPAATLIRILDAHPSLI"
                     /name="nadS"
     gene            15771..17246
                     /gene="msr(E)"
                     /locus_tag="RQL83_RS19380"
                     /old_locus_tag="RQL83_19440"
     ARG             15772..17243
                     /name="msr(E)"
                     /gene="msr(E)"
                     /gene_name="ABC-F type ribosomal protection protein Msr(E)"
                     /tool="amrfinderplus"
                     /drug_class="MACROLIDE/STREPTOGRAMIN"
                     /identity="100.0"
                     /coverage="100.0"
     gene            17302..18186
                     /locus_tag="RQL83_RS19385"
                     /old_locus_tag="RQL83_19445"
     CDS             17302..18186
                     /locus_tag="RQL83_RS19385"
                     /old_locus_tag="RQL83_19445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(E) family macrolide 2'-phosphotransferase"
                     /protein_id="WP_000155092.1"
                     /translation="MTIQDIQSLAEAHGLLLTDKMNFNEMGIDFKVVFALDTKGQQWLL
                     RIPRRDGMREQIKKEKRILELVKKHLSVEVPDWRISSTELVAYPILKDNPVLNLDAETY
                     EIIWNMDKDSPKYITSLAKTLFEIHSIPEKEVRENDLKIMKPSDLRPEIANNLQLVKSE
                     IGISEQLETRYRKWLDNDVLWADFTQFIHGDLYAGHVLASKDGAVSGVIDWSTAHIDDP
                     AIDFAGHVTLFGEESLKTLIIEYEKLGGKVWNKLYEQTLERAAASPLMYGLFALETQNE
                     SLIVGAKAQLGVI"
                     /name="Mph(E) family macrolide 2'-phosphotransferase"
     gene            18438..>18983
                     /locus_tag="RQL83_RS19390"
                     /old_locus_tag="RQL83_19450"
                     /pseudo=""
     CDS             18438..>18983
                     /locus_tag="RQL83_RS19390"
                     /old_locus_tag="RQL83_19450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611903.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /name="helix-turn-helix domain-containing protein"
     gene            19062..>19699
                     /locus_tag="RQL83_RS19395"
                     /old_locus_tag="RQL83_19455"
                     /pseudo=""
     CDS             19062..>19699
                     /locus_tag="RQL83_RS19395"
                     /old_locus_tag="RQL83_19455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032072965.1"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /name="IS3 family transposase"
     ARG             17303..18183
                     /name="mph(E)"
                     /gene="mph(E)"
                     /gene_name="Mph(E) family macrolide 2'-phosphotransferase"
                     /tool="amrfinderplus"
                     /drug_class="MACROLIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             11294..12132
                     /name="blaOXA-58"
                     /gene="blaOXA-58"
                     /gene_name="carbapenem-hydrolyzing class D beta-lactamase
                     OXA-58"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             15772..17246
                     /name="msrE"
                     /gene="msrE"
                     /gene_name="msr-type ABC-F protein"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic; streptogramin
                     antibiotic"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             17303..18186
                     /name="mphE"
                     /gene="mphE"
                     /gene_name="macrolide phosphotransferase (MPH)"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic"
                     /identity="100.0"
                     /coverage="100.0"
ORIGIN
        1 ggtgatctga atagattata caaatgaaca tatatttata tgaattatta aaaagtgaat
       61 ttctataagt ttaatagtcc gttttttcag ctttataaaa catacctccg tctatggaaa
      121 ttaaattcat gcaatatccc tccagtatta gactgtcaaa ttattcattt tatcctaagt
      181 agaaatttct cttagaataa gtaaaaacag tattacttta gaaaatttga ttccctatta
      241 gctcaaaaat ggcacatgcc attgattata ttaaaattaa taaattaaat ttgacaattt
      301 ttacatatga acatatattc atatgtaaaa atatatacat gttaggtttt tatacatgag
      361 taatcaacgc gatcacaatc ataaatgtca tgatcattcg catgaaggtc atgatcattc
      421 gcatgaaggt catgatcatt cacatgttcc tgatgtgagt aacggaaacg aaagaaaaat
      481 tctgatttcg ttttttatca ttttcgggtt catgcttgta gaagccactg gaggaatcat
      541 ctctggttca ctcgcacttc tggctgatgc agggcatatg cttacagatg ccgttgcctt
      601 aggtttagct tatgctgcct ttcgtttcgg tcgccgcatt gcagataatt caaaaacatt
      661 tggatacatg cgattcgaag ttattgctgg cttaatgaat tctcttacat tattcgcaat
      721 cgttggatgg attatttatg aggctattga acgctttcaa tcacctcaac ctgtcatggc
      781 aggaccgatg tttatcgttg ctattatagg tatgctcgtt aacttatttg ttttatggta
      841 cttaacaaga ggcgatacag aacatgtcaa tgttaaagga gctattctcc atgtaatggg
      901 cgatttactc ggctctgttg gagcaattat tgctgccata gtgatttggt acacaggctg
      961 gactcctatc gatccaattt tatctgttct agtttcttta cttgtactac gcagcgcatg
     1021 gtctttattt aaacgtactc ttcatatttt acttgagggt gctcctgaga atgctaatgc
     1081 ccaagagatc actgaatatc ttttgaaaaa tgtaagtggt attgaaaaaa ttacccatat
     1141 acatatttgg atgattacat caggtagatc tatggcaacg ttgcatgtca aacccaaaga
     1201 tggagttgac ctcgcgacat taactctcaa cattgagaat gagctaaaat ccaaattttc
     1261 tattgaacat ccaactatag ccattgattg gcaaggcaat agtacttgta gcttagaaaa
     1321 cccaactcaa attgatccac acgctggaca taatcactaa ccttaaatct ttattgttat
     1381 tcactgatga ggagacttta atgtctgata aatactacaa tttaggagat taccagcgtc
     1441 aaataactac gtcatctcct gatactcaac tttggtttga tcgtggctta ttatggacat
     1501 attgtttcaa tccgagtgaa gccataaatt gttttaagaa agctctagaa catgatgcaa
     1561 aatgcgcaat ggcttattgg ggcatcgcct ttgcatctgg tccaaattac aataaagctt
     1621 ggcatttatt tgatccttca gacttacaga ataccattcc atatattaat gaaattatgg
     1681 aacaagcaaa gttagcagcg actcataatg cgacacaatt tgagaaagat ttaattgatg
     1741 cccttgctcc acgctttcca aaacacgtga ctatagatga acaagacttt catgagctga
     1801 attttgcata tgctcaagct atgagatcat tttatgaaaa atattctaat gatcttgatg
     1861 cagctgcaat gtttgcagat gcactcatgt gcttgagccc tcgtaaattg tgggatttag
     1921 ataccggtga agccataggt ttaggtacag ttgaagctag aaacgtgctt gaagttgctt
     1981 tcactaaacc tggggcaaat cgtcatcctg taattaatca tctctatata catttaatgg
     2041 aaatgtcacc ttatcctgaa gttgcattgc cagctgctga taaccttcgt aatttatcac
     2101 ctgatgggtc acatttagcc catatgtcca cacatattga taatgcatgc ggagattggc
     2161 gtcgagtggt ttattctaat cttgatgcaa tcgcagccga tgaaagatat ttttctagag
     2221 aaaagaatcc aggtggttgg aatatcttgt atcgcgttca taatttaatg gtaggtgctt
     2281 atggtgcaat gatggcaggt aaatcacagg aagcgttatt tatggctcat cgtatcaatg
     2341 acattttaac acccgaagta ctacatatca ctacaccgcc aatggctaat ttaactgaaa
     2401 gccattgcac aacactacca catgttatga ttagatttgg catgtgggaa gatattctga
     2461 aacttgagct acctaaagat aaaggtctat atagttccac aaccgcaatg attcactacg
     2521 caaaaggcat agcatttgct tcattaagaa gagttaaaga ggccgaaaaa gaaagagagt
     2581 cttttataaa agcagtagct aatgtatcag aagagcgctt taactctata cctgttaaag
     2641 aggtcgaagc tttaaaaatt gctgccaaaa tgattcatgg cgaaattgag tatcgtaaag
     2701 gtaatacgaa agaagcattc gcaatcttgc gagaaggcat taaattagaa gatgctcttc
     2761 cttattgtga tcctccagct tggttacaac cagttcggca tgcattctgc gcacttaata
     2821 tggaacaagg aaactttgaa gtagcagccc aagaatatcg gattgaactg gggctagaag
     2881 gtgaccttgg acgtcgccga attcgcccta acaatgtatg gagtcttcat ggactttatg
     2941 agtgtttagc aaaactaggt ctagagaaag aagctcgaga tatttcgctg caacgtgata
     3001 tagcacttgc ctcggctgat attaatattg aagcatcctg tttctgccga ttaagtgcat
     3061 ttgaagataa cactacaagt tgctgttaaa aacagatagc actttcctca aatagatatc
     3121 aaggcatata gctagacatt tcttaattat atgcccttga tcttaacttt ttgtagatga
     3181 tactgataac ttaaataaat gcatgatata tttcttgttt agagtaataa aaataaagaa
     3241 ttcctataac cctctttccc cttaaagtta tttaatttag atatttttgc aatacattac
     3301 gtactactat aaattagtgc tcctcactaa tatgacttgc catatctaat aaaacctgac
     3361 taacatgtaa atcatcaatc tcataaaata cttgtttaga ttgtctttcg cctttgacta
     3421 aacgagctga acgcaataaa cgaagattat gactgactaa agattgggat aaatcaagag
     3481 catcactgat ttcacctact gaactaggcc cccgcataca aaaaagtaga attttaagtc
     3541 gtgaagcatc acctaatagc cgaaatgttt cagctaaaga cataatctca tcttgagata
     3601 atggtatcgg gtgattatta aaattcatac taatacttaa attcctatta tttagaaaat
     3661 atatatgttt acatattcta cccttgacct tcccataatg gcaaggttta tgctcttaat
     3721 aattgggtaa gcactatagg tatatcttat gggaaaggca acagataagc tttctaataa
     3781 tatgaaagtt acattaaaaa ttgaagggat gacatgtgcc agctgtgttg ggcgtgttga
     3841 aacagcactc aaaaaagttg atggtgtaca gtctgcctcg gtcaatctag cgacagaacg
     3901 tgcagatatt gtacttgatc agcctgtgga tcgtcaggta ctggttcatg ccattgaaca
     3961 atcaggttat gacgtgcccg caaataatat tgaattatca attaaaggca tgacctgtgc
     4021 tagttgtgta ggtcgtgtcg aaaaagccct taaagccgtt gcaggtgtaa aagaggccaa
     4081 tgtcaatctt gcgactgagc gtgcaacggt cagtggttca gcaaatgtcg atgacttgat
     4141 taccgcaatt gataaagctg gttacgatgc cacagaaatt caagcttcta ttcctgacca
     4201 gacagagctg cttgagaaaa aagatcaaga acgtgctgaa cttaagcgtg atttgattct
     4261 tgcaacccta ttggctttgc ctgtatttat tttggaaatg ggatctcacc ttatccctgg
     4321 tgttcatcaa ctgattgatc aaactattgg gatgcagaat agctggtatt tacaattcat
     4381 cctgaccacg ttggttttag ttattccagg acgaagattc tatctaaaag gtttgcctgc
     4441 attactccgt ttagcacctg atatgaactc gctggttgcg gttggtacat tagcagctta
     4501 cctattttct attgttgcca cattcatacc caacatcctt cccacaggaa cggtaaatgt
     4561 atattacgaa gctgctgcgg tgattgtggc attaatctta ctgggtcgtt ttcttgaagc
     4621 aaaagccaaa gggcgaacat ctgaagcaat ccagcgttta gtcagcttac aggccaaaac
     4681 tgcccatgtt ttacgcgatg gccaaatggt agatattgca ctagaccaag tggttgcaga
     4741 tgacattatc attgtgaaac caggtgaaag aatccctgtc gatggtcaag tcactgatgg
     4801 aaaaagtttt gttgatgaat ccatgattac gggtgaacca atccctgttg agaaaggcat
     4861 cggcagtgat gtggtaggcg gcaccattaa ccagaatggc acgttgacct ttaccgctgt
     4921 ggcggtgggt ggcgaaacta tgctggctca aatcattcgt ttggttgagc aagcacaggg
     4981 ttctaaaatg ccgattcaag ctgtggtgga taaagttacc ttatggtttg tccctgcggt
     5041 gatgattgcc gcagtactta cattcctagt ctggcttatt tttggtccat caccagcctt
     5101 aacctttgcg ttggttaatg ctgtggcagt gctgatcatt gcctgcccgt gtgcaatggg
     5161 attagcgaca ccaacctcaa ttatggtggg tacaggtcgt ggtgcagagt taggagtgtt
     5221 gttccgtaaa ggtgaagcct tacaactatt aaaagatgct aaagttgttg cagttgataa
     5281 aacagggacg ttgacagagg gtcatcctgt gctgactgac tttgaagtaa cccaattgtt
     5341 tgagcgtgat tatgttctag gcatggttgc ggctgtcgaa tcccgttctg aacatccgat
     5401 tgctaaagca attgttgatg cggcaaaaag tgaaaaccta gctctaccta aagtcgatac
     5461 ctttgattca gtcactggta tgggtgtcta tgcaaccatc aatggtaatg aaaaaatcaa
     5521 tattggtgca gaccgttata tggttcaact tggtattgat gttgctccat ttgctgttac
     5581 agctcagcgt cttggtgatg agggtaaatc ccctctttat gttgctgtcg atggcgaact
     5641 agcaggaatt attgcagttg ccgacccaat caaatccaca acgccagcag cgattaaggc
     5701 tctacaccaa ttagggctta aggtggcaat ggttactggt gacaatgttc gtactgctca
     5761 agcgattgcc aaacaattgg gtattgatga agtgattgct gaagttttac cagagggtaa
     5821 agttagtgcg gttaaagagc tgaaagcaaa atatggccat atcgcttttg tgggtgatgg
     5881 tattaacgat gcgccagctt tggctgaagc tgatgtggga cttgcgattg gtacaggtac
     5941 agatgttgca attgaatctg ccgatgtcgt gttaatgtca gggaacttac aaggtgttgc
     6001 caatgcgatt gccttgtcca aagcaacgat tggtaatatt catcagaact tattctgggc
     6061 atttgcctat aacaccatgt tgattcctgt tgctgctggt atattgtacc cagcctatgg
     6121 aatactgatg tccccaatct ttgcagcagg tgcaatggct ttgtccagtg tatttgtact
     6181 cggtaatgca ttacgtttac gtcgattcca gtcaccatcc gtcagtcaag gctaaggagg
     6241 atatgatgaa cattggtaaa gtctctaaag cttcaggagt taccgccaag atgattcgtt
     6301 attacgagca gattggcctg attcccgctg ctggacgtaa taatgcagga tatcgctctt
     6361 actcagtaaa tgatgttgaa cgtttaaagt ttatcaaacg aagtcgtgaa ctcggttttt
     6421 cagtagcgga aatatcagac ctactcgatt tatggaataa ccgcagtaga cagagtgctg
     6481 atgtcaaacg cctggctcag ggtcatattg aaaagctgga agaacgtatc aatgacctac
     6541 agcaaatggc gaatactctc aaggacttaa tcgactgttg tgctggtgat aatagacccg
     6601 actgtccgat tcttgaaggc ttgcaagaac ctcacaatga aataaaggac gttcaccatc
     6661 ataacgacct gctttccgtt tcacctttaa cccgaaagaa atagcaatag gatataggga
     6721 gaagtaaaat taattttact tctccctatt ttttatattt ataactatga actaaaatat
     6781 gtcatttctt ttagatactt acattgtcaa ataactgcta atttaacatc acaaacattc
     6841 ctaaatattg agcttataat tcattgccca caaaaaagcg actcaataga gagtcgcttt
     6901 atcatttcgt ccgtttttaa cgaggaggaa aaccagcctc ggttaaagcc tcaacgattt
     6961 gctgctgtgt tgcagttgtt tcaactttta cactacgatt gggtggttcg gtgatgacct
     7021 ttgcattggc atctattgct tgaattgctc tggttacacc acgggcacag ccaccacaag
     7081 tcatatcttc aacatgaaat tccatattta actccactat tactattcag gatgtgttta
     7141 atataaacct tgccataatt gtaaggtcaa gcattaaaag taaaattaat gtccctcaga
     7201 tatataattg actgtgtact taggtatttc tacctccaag ttttcatctt ggaccttaac
     7261 ctggcaagat agtcttgatt gggccgtaag cccccaggca cgatccagta agtccgcctc
     7321 tacatcatcc atttcttcta aactgtcgaa cccttttttc agaacaacat ggcatgttgt
     7381 acatgcacct gataaatcgc aggcatgttc tatgggaatg ccatgatcca ataaacttcg
     7441 acataaatta cttccgcttt caatttcaag ttccgcaccc tctggacaaa tcttttcatg
     7501 cggaataact ataatttttg gcatataaca taatcctcaa ttcatcaaat ttatagataa
     7561 aacaaggggc tttaaaaagc ccctcattta aaagaatatc gaatagagaa gtatctactt
     7621 atgtttactt aaccattcat tcatcatctt gatttctgat tcctgggcag agataacatc
     7681 ttcagcaagc tggcgtactt ctggatcaga accatattga agaactactt ttgccatatc
     7741 aattgcacct tgatgatggg ggatcatgcc tctcatgaaa tcaacatcag catttcctgt
     7801 aaatggaacc atcatatctg cgtgcatttc atcattgatc ttaatatatg cttgagttga
     7861 tgggctatct tcatttttgc tgccagaatg atttttgttt tttgccaacc attcattcat
     7921 catcttgatt tctgactctt gggcagagat aacgttctcg gcaagctgac gcacttctgg
     7981 atcagaaccg tattgaagaa ctacttttgc catatcaacc gcaccctgat ggtgaggaat
     8041 catacccaac ataaagtcag tatctgcatt tcccgtgaaa tcaataccca ttccgctgtg
     8101 catacgagta tttacttcca tataggcatt tgtagatgga ttatctgttt tagctgaacc
     8161 attcatatca tggtgcattg aatggctttc atgtttattc tcactcattg aattttcctt
     8221 tggagcgtca gaagaggaac accccgcact aaatacaatc gcaccgatca ttaacattgg
     8281 aacaaagtgt ttcatggagt tatcctctta ctacaattat gaaaaaataa tgggtaataa
     8341 gctcggaagt tattctttca tatcaggtac atcattaccc ttgatgtagc aactctattc
     8401 tttccaatta tggtaaggtc aacacctaat caaataaaaa taattaaaag aataaaacaa
     8461 ttaaaaacaa atacttaaag acaattaact ccgtagtgag tcacaataat caatagaatt
     8521 aaaaaaatca cctccaatca caaataatat ttttatataa atatcaatgt gatatgtgtc
     8581 ttcatggttt cacaattatt tatttgacta ttggatgcac tacaaaagac tacaaacatt
     8641 agagatggat ccggaaattt gaacaactcc tataagtgat actctgctcc tcaaatgatg
     8701 ttataaacat caatatatgg agtattttat ggcacgtaga ccaagaagaa atcattcaaa
     8761 cgactttaag gctaaggtag cacttgctgc gatcaaagca gaaaaaacac ttgctgaatt
     8821 gagttctgag tttgatgttc atcaaaacca aattattgac tggaaaaatc aattgatctc
     8881 agcttcctca caagctttcg atcaatcaaa agctccatca gaacctccca ttgatcttaa
     8941 aaagttacat gcaaaaatcg gtgagcaggc attagaaatt gattttttag aaggtgtgtt
     9001 gaagaaactg ggccgcttca accacaaaag ttaatcgatc actcacttca gatttcagta
     9061 tctaaacaag ctaagttact gaaagtctct cgtggttgtt attattatcg cccaaaacct
     9121 gttagctcat cagatctgaa gctgatgcga tgtattgatg aattacatat gcaatatcct
     9181 tttgcaggca gtcgtatgat gcgtgatttg ttgaatcgtc aagggcatca tataggacga
     9241 cgtcatacac gtactttaat gaagaaaatg ggtattcagg cgttatattg caaaccaaat
     9301 ttaagccagg ctaatcaagc tcaccgtaaa tatccatatc tgctcaaagg attggctatt
     9361 cagcgcagta atcaagtgtg gtctactgat ataacgtata tccctatggc aaaaggcttt
     9421 gtttatttat gtgctgtgat tgattggcat agccgcaagg tacttgcgca tagggtatcg
     9481 attagtatgg aggtggattt ttgtatttcg gctttaaatg aagcgattga aaaatatgga
     9541 tcacctgaaa tattgaatac agaccaaggc agtcagttca ccagtgatgc atttattgat
     9601 gtattgaaat caaatggcat tcaaatcagt atggatggta aaggtcgatg ggtagataat
     9661 gtgatggttg aacgattatg gcggagcgtt aaatatgaag aggtgtatct caaagcttat
     9721 agcagtgtca cagatgcgaa aaagcaattg agtgcatatt ttgagtttta taatctgaaa
     9781 cgacctcatt cgagtctaga caaaatgaca ccaaatgagt tttactatga tcagctaccc
     9841 caacaaaaca aggtggctta actagagcgg aatatcactt ataaatacgc ttttagttgt
     9901 tcaaacaagt ggaaccacct ctattatatg atttcaacac gcttgtatgt tgagcagcat
     9961 ggtaagccgt tggcatttta cagcgacaaa cattcagtct tcagggtgaa tcaaagcagc
    10021 aagaaagaca ccaagattac ccagtttgga cgcgtactca gtaccctgaa tatcgatatc
    10081 atcttcgcca attcaccaca ggccaaaggc cgtgtggaac gtgccaacag aacccttcag
    10141 gaccgtctga tcaaggagat gcgtctggaa ggtatcagct cgattgagca agctaatgcc
    10201 tggctaccct gcttcattga gcagttcaat cagaagttcg ccaagatggc ttttaatcct
    10261 aaggatctac atcggcctat cactgaaaca gccgaagaat tagatgatat ttttacttgg
    10321 cgtgaacccc gcagagtcac gaatagcctg acgattactt atgataaatg cgtatatctc
    10381 ctggaaaaca ccgaagaaaa tcaaaggttg atcggtaagt atcttgagtt cctggaatac
    10441 ccggatggta ctgtagccct catgcatgat ggccgaaaga tcaattacag cctcttcgat
    10501 aaattaagtc agctgaatca gcgagagatt gttgagaata aacggttggg tgctgttctg
    10561 aatcatatcc aacaacagca tgaagaactg gaacaacaaa acaaacgcaa tcgttctcaa
    10621 aagatgccaa gcagacgtgc acagaaaata gcaatcaaag aacgaaatct aaatcctgtg
    10681 cttgacttgg aattgtccat ataggacatt tctatttggt tattaggtag gacatttcta
    10741 cttaggaata acaccacaga gagtgttaga acccatttaa agtgtctata ttctcaccaa
    10801 taaaaatgtc cggtttctcc gaggtaaaat atgacttttg aacaaaaata agccattgtt
    10861 tattcttttt atttccaaca aaattccaga actcatctac ttcgagagat tcataatgac
    10921 tttgctgagg ctgaatttca taggttgatt cagttaaagt acgtaaaact ttaccgatac
    10981 tgatgcgctc aacttcagca atatctcgta taccgctgcc tctgaccatc aactgtaata
    11041 ttttacgagt aatacctgac ttacatccta gatagctcag tgcatgatca ccaataaact
    11101 gacgtttaca gtctttgcac tgatagtttt gtttcccatc tactttgata ccatttttct
    11161 ttatactatc actgaggcag gttggacatt tgattgctag agttatttgc atttctctat
    11221 tttatcaaaa tccaatcggc tttttcttca gcatactttt tgaaacacta ccaaatttta
    11281 aagttgtata tcatgaaatt attaaaaata ttgagtttag tttgcttaag cataagtatt
    11341 ggggcttgtg ctgagcatag tatgagtcga gcaaaaacaa gtacaattcc acaagtgaat
    11401 aactcaatca tcgatcagaa tgttcaagcg ctttttaatg aaatctcagc tgatgctgtg
    11461 tttgtcacat atgatggtca aaatattaaa aaatatggca cgcatttaga ccgagcaaaa
    11521 acagcttata ttcctgcatc tacatttaaa attgccaatg cactaattgg tttagaaaat
    11581 cataaagcaa catctacaga aatatttaag tgggatggaa agccacgttt ttttaaagca
    11641 tgggacaaag attttacttt gggcgaagcc atgcaagcat ctacagtgcc tgtatatcaa
    11701 gaattggcac gtcgtattgg tccaagctta atgcaaagtg aattgcaacg tattggttat
    11761 ggcaatatgc aaataggcac ggaagttgat caattttggt tgaaagggcc tttgacaatt
    11821 acacctatac aagaagtaaa gtttgtgtat gatttagccc aagggcaatt gccttttaaa
    11881 cctgaagttc agcaacaagt gaaagagatg ttgtatgtag agcgcagagg ggagaatcgt
    11941 ctatatgcta aaagtggctg gggaatggct gtagacccgc aagtgggttg gtatgtgggt
    12001 tttgttgaaa aggcagatgg gcaagtggtg gcatttgctt taaatatgca aatgaaagct
    12061 ggtgatgata ttgctctacg taaacaattg tctttagatg tgctagataa gttgggtgtt
    12121 tttcattatt tataagaatt agaagtttga ggttaatcta tttttggtag tgtttcaaaa
    12181 agtatgctga agaaaaagcc gattggattt tgataaaata gagaaatgca aataactcta
    12241 gcaatcaaat gtccaacctg cctcagtgat agtataaaga aaaatggtat caaagtagat
    12301 gggaaacaaa actatcagtg caaagactgt aaacgtcagt ttattggtga tcatgcactg
    12361 agctatctag gatgtaagtc aggtattact cgtaaaatat tacagttgat ggtcagaggc
    12421 agcggtatac gagatattgc tgaagttgag cgcatcagta tcggtaaagt tttacgtact
    12481 ttaactgaat caacctatga aattcagcct cagcaaagtc attatgaatc tctcgaagta
    12541 gatgagttct ggaattttgt tggaaataaa aagaataaac aatggcttat ttacgcctat
    12601 catcgagaaa caggtgaaat tgttgcttat gtttggggta aacgagattt agctacagtc
    12661 taacaattga agacaaagct taaacaatta gatattcact acacccgaat tgcaagtgat
    12721 cattgggaca gtttcatcac tgcttttaaa aatggtaagc aaagtattgg taaatttttt
    12781 actgtaggta ttgaaggcaa taattgcaaa ataaggcata gaattaggcg tagtttcaga
    12841 agaagctgta atttttcaaa aaagcttgaa aaccatttta aagcttttga tttagccttc
    12901 ttttctttta catcaataat ggcttcattt aaaggcagca tactttttga agcaccacca
    12961 aaattttatg ttaagtagag actaaaaatg cccttgatta agggcttaca agggcatttt
    13021 gtataaggtg tattatgtta attttagaat ttcttcatta gataattttt gataagctaa
    13081 atccattagg actaattaag taaatatgag caattgggtc aatgtcacac aggataagtc
    13141 tacaggtata gaactaattc atgcccactt caaaggcttt gcatacgatc ctcatctaca
    13201 ctcaagttat cttatagggg taacagagtt aggacatcaa caattcaatt gtagaaaaaa
    13261 aattattgat agctatcaag gtcaaacttt catgcttgaa cctgaagaag ttcatgatgg
    13321 taatgcacca gatcctctcg gcttcactta taaaatgatg catttagatc cagcatggtt
    13381 aaaaaaaagc tatgaaggaa tatttaatga gcctatcgaa cttgctatcg aatcaaccct
    13441 acgttctgat cctcaacttt cacatttgat actttcaacc tacaatatat tgaataacaa
    13501 tgaatctcaa ttgatgaaag atacgtattt agatctttta ttagaaaact tagtaaataa
    13561 aaaaatttta tataaacgtg aaaagtctat ccagacatta cctaatattg cattaaagct
    13621 aaaagaaatt ttacatgaaa gtctctttca tgatttagac ttacaaacac tctcaacatt
    13681 ggtgaaaaca gatcgttttt atattaaccg tgtatttaaa aaaaacttta actgttctcc
    13741 acatcaatat ctgatccaac tccgtttaaa taaggcaaaa gaattgttaa gaaaaggatt
    13801 atcagcaaca actgttgctt catatctttg tttttctgat caaagtcatc taggtcgatg
    13861 gtttaaacgt tgttatggca taacattgaa tcaatatcaa aaagcgtgca caaatgttct
    13921 agatatatct tgaagatatt tatcaaacta gccaaatagt ttattaattt ttggatttta
    13981 ttttggaaac gattttagct ttttgtgcct tttcttttgt tgcatcaatt actcctggac
    14041 caaccaattt ccttattctt agtacaagtc atcaatttag tattaaaaaa acattaggct
    14101 taatcttcgg tggaagtatt ggtgctgcat cattagtctt aattacagga cttggaattg
    14161 gtacaacact caatgaatac cccaagattc aattaatatt aagtttcact ggcggcattt
    14221 ggttaagcta tattggatgg aaaattttta attatagacc tgatctagaa tctaaaatcc
    14281 aaaatcaaaa taagaaaaat ggttttttta caggcttctt tttacagttt atcaacccta
    14341 aaagctggtt gatggctatt gctgttatta ctgtttatac agcacaacta gctaactact
    14401 atcagtcact cattctattt tcattcatct ttatgctgat ttctctacct tgtttattta
    14461 tttgggctta tttaggaaaa atcacaaaaa aaatactttc tacagctcat catatggtgc
    14521 tctttaatcg agtattaggt actgttttac ttatttcagt atggtatcca atattgcatg
    14581 ctatttaaca taatggctgt tatgcgaaac atagagagag atctaccagc tctcgttttt
    14641 tgttatggaa actgctaaaa ttcaaatacg ccattgacgt ataaagagtc catgttattt
    14701 attgaaacca gcatctttac caagcaaatt aaagaccttg tatctgatga ggaatatcgt
    14761 caacttcagc aagatctttt ggtacagcct gatagaggtg acctaatcaa gaatggcggt
    14821 ggtattcgca aagtacgttg tgctcaaggt aacaaaggga aaagtggtgg gatacgggtg
    14881 atttattatt gggtcaccga ggatgatcag atctttttct tagtggctta tccaaaatca
    14941 gtaaaagata atttaacaga taaggaaacc gccattctgc accaactcgt gaaggagcaa
    15001 tttcatggat aacaacttat ttgatgactt ggttgcttca atcaaagaag ctggagctat
    15061 caaacgtaaa gaagttaaag caagccgagt tacagaactc gaattgcctg atattaaaga
    15121 agtacgtgaa aaaactggct tgagccaaaa tgaatttgct gcacgtctac atattagccc
    15181 ccgtaccctg caaaattggg aacagggacg acgctatcca actggaccag cagctacatt
    15241 gattcgaatt ttagatgctc acccaagcct tatttgaaaa aattacttaa aaaatgggag
    15301 cttaaggctc ccattttttt attcgtataa ggtgtattat gttaatttta gaaaatcttc
    15361 actactatta ttttaaaata agccttatat ccaagcataa aacaaggttg tctagacttc
    15421 ttttaacagt aaagttatca taaaactgaa ttttattttt taggtaagtt tatgcattct
    15481 atccgcattc gttaagacac aactatttgc atagtgacac tattttataa tggtgggctt
    15541 ttgttgtgtc tttaagaata tatgcggata tataaagtaa aagtatgctt aatttataag
    15601 tatgctttta gtgcatagtt tccagttata acttaattga ctagctattt gtccaccctg
    15661 tggatgaata gctttttttt tgggaggaca ctgtgatgct agcttttgtt ttcacctaaa
    15721 tcctgtttgc tgcataaaaa atttcaagag ctaaacagga gtaaataaaa atgagtttaa
    15781 ttattaaagc gagaaacata cgcttggatt atgctgggcg tgatgttttg gatattgatg
    15841 aattggaaat tcactcttat gaccgtattg gtcttgtggg tgataacgga gcaggaaaga
    15901 gtagtttact caaagtactt aatggcgaaa ttgttttagc cgaagcgaca ttacagcgtt
    15961 ttggtgattt tgcacatatc agccaactgg gcggaatcga aatagaaacg gtcgaagacc
    16021 gggcaatgtt atctcgcctt ggtgtttcca atgtacaaaa cgacacaatg agtggcggag
    16081 aggaaactcg tgcaaaaatt gctgccgcat tttcccaaca agtacatggc attctagcgg
    16141 atgaaccaac cagccacctt gatctcaatg gaatagatct acttattggt caacttaaag
    16201 catttgatgg agcattactt gttatcagtc atgaccgata ttttcttgat atggttgtag
    16261 acaagatatg ggagttaaaa gacggtaaaa ttacggaata ttggggtggt tactcggatt
    16321 acttgcgtca aaaagaagaa gagcgacaac accaagccgt agaatatgag ctgatgatga
    16381 aggaacggga gcgattagaa tctgctgtgc aagaaaaacg ccagcaagct aatcgattag
    16441 acaataagaa aaaaggagaa aaatccaaaa actctaccga aagtgctgga cgacttgggc
    16501 atgcaaaaat gactggcacc aagcaaagaa aactgtatca ggcagctaag agtatggaaa
    16561 agcgtttggc tgcattagaa gatattcaag caccagagca tttgcgttct attcgttttc
    16621 gtcaaagttc agccctagaa ctgcacaata agttcccgat tacggcagat ggtctgagct
    16681 taaaatttgg tagccgtact atctttgatg acgctaactt tataataccg cttggcgcta
    16741 aagtcgctat aactggatcg aatggaacag ggaaaacgtc cttgttaaaa atgatatcag
    16801 aacgtgctga tggattaacc atatctccaa aagctgaaat tggctacttt acacaaacag
    16861 gatataaatt taacacgcat aaatctgtgc tctcctttat gcaggaagag tgcgagtaca
    16921 cagttgcgga aattcgtgca gtattggctt caatggggat cggagcgaat gatattcaaa
    16981 aaaacttatc cgacttatcg ggaggtgaaa tcatcaaact gcttttatcc aaaatgcttt
    17041 taggaaaata taatattttg cttatggatg aaccaggaaa ctatcttgac ctaaaaagta
    17101 ttgccgcatt agaaacaatg atgaagtcct atgcaggaac tattatcttc gtatctcatg
    17161 acaagcaatt ggtcgataat attgctgaca ttatctacga gatcaaagac cacaaaatca
    17221 tcaagacttt tgagagagat tgttaatgat agccaatcta atccgaacat taattattga
    17281 actctttaaa ggaaattaaa aatgacaatt caagatattc aatcacttgc tgaagcacac
    17341 ggcttgttgc ttacggacaa aatgaatttc aatgaaatgg gcattgattt taaggtcgtt
    17401 tttgctcttg atacaaaggg gcaacaatgg ttgctgcgta ttcctcgtcg tgatggcatg
    17461 agggaacaaa tcaagaaaga aaaacgcatt ttagaattgg taaaaaaaca tctttctgta
    17521 gaggttcctg attggagaat ttcatctaca gaattagtgg cttatcccat acttaaagat
    17581 aatcctgttt taaatttgga tgctgaaacc tatgaaataa tttggaatat ggacaaagat
    17641 agcccgaaat acataacatc tttggcaaaa accttatttg aaatccatag tattcctgaa
    17701 aaagaagttc gggaaaatga tttgaaaatt atgaaacctt cagatttaag acctgaaata
    17761 gcaaacaatt tgcagttagt aaaatctgaa attggtataa gtgagcaatt ggaaacccgc
    17821 tacagaaaat ggttggataa tgatgttcta tgggcagatt tcacccaatt tatacatggc
    17881 gatttatatg ctgggcatgt actagcttca aaggatggag ctgtttcagg cgttattgat
    17941 tggtcaacag cccatataga tgacccagcg attgattttg ctgggcatgt aactttgttt
    18001 ggagaagaaa gcctcaaaac tctaatcatc gagtatgaaa aactaggggg taaagtttgg
    18061 aataaactat atgaacagac tttagaaaga gcagcggcct ctcctttgat gtatggttta
    18121 tttgccttag aaactcaaaa tgaaagcctt atcgttggag caaaagctca gttgggagtt
    18181 atataattta aaaatatgat tgctgagaac tgccttgttt tgaaacttgg ttggctttaa
    18241 ttagttttta gtattcttta tagaaaatgc ctcgatcaag gggcatttct aacaatcatt
    18301 taacataaaa tttcttatgt gaagtaacag tgtaactcca aaatagaaat gtccggtttc
    18361 tccaaagtaa aaatgtccgc ttttagaata tgcacttttg caatttcgtt agcggacggt
    18421 ttgatatgtt ggtgtctatg tcggataaag aacttaaacg attgtcggtc ttgcaagaaa
    18481 tctgtgatca acgcataact caatcccagg ctgctcagct acttcatatt tcagaacgtc
    18541 agatcagacg cttactgcag aaatacaaag ctcaaggtcc cgctgcatta gcccatgccg
    18601 gtcgtggcca aatcagcaat tccaaacttc ctgaagaact cagactcaag tgcctcaata
    18661 ttgtttctga ccaactccat ggtttcggac ccactttagc gcatgaaaag ctcaccaccg
    18721 tacatggatt cgatctttca gtagaaaccc tgcgttcctg gatgattgca gctgacttat
    18781 ggattcctcg atccaagcgc ctgaaacgcc cgtatcagcc tcgttacaac cgggattgct
    18841 ttggtgaact gatccaaatt gatggctcat atcatgactg gtttgaagga cgcgctgcta
    18901 aatgctgtct gctggtgttt atcgatgatg ctacaggaaa attgcagcat ttacgcttct
    18961 gtgaatcaga atcagccttt gattaagaga tggatccgga aatttgaaca actcctataa
    19021 gtgatactct gctcctcaaa tgatgttata aacatcaata tatggagtat tttatggcac
    19081 gtagaccaag aagaaatcat tcaaacgact ttaaggctaa ggtagcactt gctgcgatca
    19141 aagcagaaaa aacacttgct gaattgagtt ctgagtttga tgttcatcaa aaccaaatta
    19201 ttgactggaa aaatcaattg atctcagctt cctcacaagc tttcgatcaa tcaaaagctc
    19261 catcagaacc tcccattgat cttaaaaagt tacatgcaaa aatcggtgag caggcattag
    19321 aaattgattt tttagaaggt gtgttgaaga aactgggccg cttcaaccac aaaagttaat
    19381 cgatcactca cttcagattt cagtatctaa acaagctaag ttactgaaag tctctcgtgg
    19441 ttgttattat tatcgcccaa aacctgttag ctcatcagat ctgaagctga tgcgatgtat
    19501 tgatgaatta catatgcaat atccttttgc aggcagtcgt atgatgcgtg atttgttgaa
    19561 tcgtcaaggg catcatatag gacgacgtca tacacgtact ttaatgaaga aaatgggtat
    19621 tcaggcgtta tattgcaaac caaatttaag ccaggctaat caagctcacc gtaaatatcc
    19681 atatctgctc aaaggattgg
//
