LOCUS       NZ_CP135119             3987 bp    DNA     linear   CON 02-OCT-2023
DEFINITION  Enterobacter hormaechei strain F2 plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP135119
VERSION     NZ_CP135119.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36017835
            Assembly: GCF_032231155.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 3987)
  AUTHORS   Zhang,B.
  TITLE     Algicidal process and mechanisms of Enterobacter hormaechei F2
            revealed by an integrated transcriptomic and metabolomic approach
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3987)
  AUTHORS   Zhang,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-SEP-2023) School of environmental science and
            technology, Sun Yat-sen University, Zhonghuan Dong, Guangzhou,
            Guangdong 510000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. 2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 99.48x
            Sequencing Technology             :: Illumina; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/02/2023 13:40:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,546
            CDSs (total)                      :: 4,430
            Genes (coding)                    :: 4,370
            CDSs (with protein)               :: 4,370
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 22 of 60
            Pseudo Genes (incomplete)         :: 40 of 60
            Pseudo Genes (internal stop)      :: 8 of 60
            Pseudo Genes (multiple problems)  :: 10 of 60
            Pseudo Genes (short protein)      :: 1 of 60
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP135119.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3987
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="F2"
                     /isolation_source="environmental"
                     /db_xref="taxon:158836"
                     /plasmid="unnamed2"
                     /geo_loc_name="China:Guangzhou"
                     /collection_date="2017-09-10"
     gene            263..376
                     /locus_tag="RPB40_RS22685"
                     /old_locus_tag="RPB40_22685"
                     /pseudo=""
     CDS             263..376
                     /locus_tag="RPB40_RS22685"
                     /old_locus_tag="RPB40_22685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            605..1042
                     /locus_tag="RPB40_RS22690"
                     /old_locus_tag="RPB40_22690"
     CDS             605..1042
                     /locus_tag="RPB40_RS22690"
                     /old_locus_tag="RPB40_22690"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_064183952.1"
                     /translation="MQQAIRMRIEADKAALDAGFTTNNFVGELGEKLAHAVYGGRILPN
                     SAKSADIQTDDGTLLQVKTRKPNKGKQFGSIRSWDFDYLIAITLGDKYEVIRALKIPVS
                     TCKELADHRAHTNSFIIKPTRKLLSHADIEDVTDKFQSVVL"
                     /name="hypothetical protein"
     gene            1294..1737
                     /locus_tag="RPB40_RS22695"
                     /old_locus_tag="RPB40_22695"
     CDS             1294..1737
                     /locus_tag="RPB40_RS22695"
                     /old_locus_tag="RPB40_22695"
                     /inference="COORDINATES: protein motif:HMM:NF013561.3"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="WP_064183953.1"
                     /translation="MIAVSLPDELLKRLDDAVDKTGKKRSFLIRESISMYLNNIENIHE
                     KKEIILNTSKPFYEVLIEEFKNEKKLITEARKTEFTMFSDNGKLYVINSKGNTRRLEPM
                     YVNNFFEEYKKTGSMSTSSYQDITFNSSYLLAALQYLMEKELI"
                     /name="ribbon-helix-helix protein, CopG family"
     gene            complement(2214..2429)
                     /locus_tag="RPB40_RS22700"
                     /old_locus_tag="RPB40_22700"
     CDS             complement(2214..2429)
                     /locus_tag="RPB40_RS22700"
                     /old_locus_tag="RPB40_22700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_064183954.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGS"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2436..2927)
                     /locus_tag="RPB40_RS22705"
                     /old_locus_tag="RPB40_22705"
     CDS             complement(2436..2927)
                     /locus_tag="RPB40_RS22705"
                     /old_locus_tag="RPB40_22705"
                     /inference="COORDINATES: protein motif:HMM:NF016710.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_064183955.1"
                     /translation="MSSLLTLAKDLEQQSKAQKQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRLSVLRTAGRTWLTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            complement(<2962..3666)
                     /locus_tag="RPB40_RS22710"
                     /old_locus_tag="RPB40_22710"
                     /pseudo=""
     CDS             complement(<2962..3666)
                     /locus_tag="RPB40_RS22710"
                     /old_locus_tag="RPB40_22710"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3605..3928)
                     /locus_tag="RPB40_RS22715"
                     /old_locus_tag="RPB40_22715"
     CDS             complement(3605..3928)
                     /locus_tag="RPB40_RS22715"
                     /old_locus_tag="RPB40_22715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_021243036.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARTGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          7..380
                     /name="rep_cluster_2373"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.553"
                     /coverage="87.0"
                     /evalue="2.31e-150"
     Typing          complement(2123..3615)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.8"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3612..3928)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.396"
                     /coverage="99.0"
                     /evalue="3.64e-61"
ORIGIN
        1 cggaaaaagg atgcggcgtt gccgtttttc cataggctcc gcccccctga caagcatcac
       61 gaaatatgac gctcaaatca gtggtggcga aacccgacag gactataaag atcccaggcg
      121 tttccccctg gtagctccct cgtgcgctct cctgttcctg cctttcggtt taccggtgtc
      181 attccgctgt tatggccgcg tttgtctcat tccacgcctg acactcagtt ccgggtaggc
      241 agttcgctcc aagctggact gtatgcacga accccccgtt cagtccgact accgcgccat
      301 gtaaggtaac tatcgtcttg agtccaaccc ttacagacac aaaaaatcgc cagtggcggt
      361 agccattggt aactgattta gaggaggaga gtcttgaagt ttcgggtgcg ataaggctac
      421 actgaaagga cagttttggt atctgcgctc ctccaagcca gttaccttgg ttcaaagagt
      481 tgctgctctg agaaccttcg aaaaaccgcc ttgctgggcg gttttttcgt tttcaaagca
      541 agagattacg cgcagaaaaa aaggaactca agaagatctt cccccattaa gggctacaaa
      601 aagtatgcaa caagccatcc gtatgagaat cgaagccgat aaagcagcat tagatgctgg
      661 ttttactacg aataattttg tcggtgaact gggcgaaaaa ttagctcatg ccgtatatgg
      721 aggtcgcata cttcctaact cagctaaaag cgcagacata cagacagacg acggcacttt
      781 actccaggtg aaaacgagga agccgaacaa aggaaaacag tttggtagca tccgctcttg
      841 ggattttgat tatctgatag cgataacgct gggtgataaa tacgaagtca tcagggcctt
      901 aaaaatccca gtatctacct gtaaagaact ggccgaccat cgggcacata cgaactcttt
      961 cataataaag ccgaccagaa aattactttc acatgcggat attgaagatg tgacggataa
     1021 atttcagagt gttgttttat aggtggtttt accccctact tgaataattc tgcacgaaat
     1081 cacgaccgta aaaaaaccgc catcttgagt agaagggcgc tattgcacaa gcatccggct
     1141 ttaccgtctt ttaccataac atcccttgca cgtacctacg aactaatcag aacatgcaac
     1201 tgattagaaa atgaaatctc gactattgac ggccgctcta tttttatata aatttagatt
     1261 agttaattag attataaatt tagagggctg ttcatgattg ctgtatcact tccagacgag
     1321 ttactaaaaa gactagatga tgctgtagat aaaactggta aaaaacgctc attcttgata
     1381 cgagaatcaa tatctatgta tttgaataac atagaaaaca tacatgaaaa aaaagagatc
     1441 atcctgaaca cctcaaaacc gttctatgag gtcctcattg aagaatttaa aaatgaaaaa
     1501 aaactaataa ctgaggccag aaaaactgag ttcacgatgt ttagtgataa cggaaaactc
     1561 tatgtcataa attcgaaagg gaatacgcgc aggctagaac ccatgtatgt gaataatttc
     1621 tttgaagagt ataaaaagac aggaagtatg agtacatcta gctatcaaga tataaccttc
     1681 aactcatctt atcttttagc tgcactacag tacctgatgg aaaaagaact gatttaacat
     1741 aagcactcat tgcccgcacc gccccggaca gcttcgccac gaacggggcg gcagtcagcc
     1801 aaaaccaaca acaacccacc cggtaaacca ccccgatcgc gacagataaa ttttacccaa
     1861 ctgcatagcc atgcagccag gtgaatgacg gcaggattca gcccaaaaac agcgtgaagg
     1921 ccccgaaaac cggcaaatgc ttctccagcc actcccacag ttctgccgcc agatgcaggc
     1981 agttaagtac agcttcaata accttaagca gtttcataat tccccctggt ataggccgtg
     2041 atctttgcat cacgacgtat ttagcgggga atttcgcagc ccttcttctt ggggtttgtc
     2101 acgggacggc atccgtcagc ccagcgtcgg tcctttgtag gttttctgcg gctggcgctg
     2161 caactccagg cttttctgat actcatgctc ccgctgccgc tgttctaccg tactcagctc
     2221 ccggacaagc ggcgcagctg ctcgcttaaa ctcagcacct gcgtgctcaa gtccgacacg
     2281 cgctcgttca gcaccgcgtt ctcccgttgc ataagaccac acatgctccg ccattccgcg
     2341 aaggcgctct cccattcgtc cagcctttgc gagtagtcct gctgtagctg ctctaatgcg
     2401 ctcagcaact gcttttccag ctctgtcatt ttccgctact cctgtttcag gcggatccat
     2461 gtcgtcccgt cgtaagggat ggcttcggtt tgcatccccg gcggcaggat aagaaacctc
     2521 cggccgtcgc tgctctcctg atagcgcact ccccacgtcc gggcggtcat tttcgccagg
     2581 gtatcttcct gctgacgaag atgcgtgtaa ttgtcggtta tctgctgccc ctgccaccac
     2641 agaatgctcc cgctcgtgcc gatcagtagc gctgacacca tcaggatggt gagccatgtc
     2701 cgcccggcag tccgcagtac gctcagccgg tttttctcca tcgcctcgct catactctgc
     2761 tcgtgtgcga ggatggcgtc gctgattctc ctcgcgcttg cgctcagttc cgctctgaca
     2821 gactgctcgt gctcgctgaa tgcggctttc agcatctcgc cggtactctg cttctgcgct
     2881 ttcgattgct gctctaagtc cttcgccagc gttaaaagac tgctcataga tggctccctt
     2941 cagtcggatg tttcgccccc cgtccgggtc ggcaatgctg atactgcttt tcgtggtgcg
     3001 caccacctca aaacctgacc gttccagcgc ctcagtgacg tcctgacggg ttttcagctc
     3061 cccggacgaa gcaagggtca gcagtccgcg cgtgatggct tctgcggcct cctgcgtcgt
     3121 tttcggcagc ccggacggcg tgatcagcgc ccggcggttt tccggtgcgt tcgggtcgtg
     3181 cagccccagt ctgccgttca cgatggtctg ccaggcatcg atacgcggcc ggtcggcgcg
     3241 gtcgtagtac ggctggagcc gcctgccggt cagcagctcg gtattgggga tcagaaagtt
     3301 cagctccaga cgccccttgt cctcgtgctc cacccacaga atgctgtact gatctttatc
     3361 aagtccgggc atcagaaccc gctcgaagct ggccatcagt ttttcccgct ggccgggagg
     3421 cagatcggcc tccgcaaacg acaggacgcc ggaggtgtat ttcttcacgt aaggcgacgc
     3481 gtcaatcagc tcccggactt cttctggctt cccctgcagg acgctggcgc cttcacgctg
     3541 ccgatcttta cccagcagat agtccaccgg cccggcgcca ccgccgcgac cgcgtggatg
     3601 aaatttaacg atcatcgtcc gttcctttct caagaacggc gtgccgcagc cgctcaagcc
     3661 cggcatcgat ggccatcagc gccgccacaa tctgcaccct gtcatggccg gtgccgccgc
     3721 cggcgttaac acggcgggct atctggttca ggttattgcc catgccggca agctggcgca
     3781 gcagcgccgg cgagattgag gggagctttc cggtgcgcgc aggcttttcg tccaggcagg
     3841 tctgccgcat ccacgccgcc agctgcctgc cgtcacagcg ctcaagcagc cgccggtgct
     3901 cgtcctccgt cacccacatg gtgagcatct tgttccgttt ctctgacaaa ccggcctcct
     3961 gataacgccg ggacgggcgg gaaagcc
//
