LOCUS       NZ_CP135118             4440 bp    DNA     linear   CON 02-OCT-2023
DEFINITION  Enterobacter hormaechei strain F2 plasmid unnamed1, complete
            sequence.
ACCESSION   NZ_CP135118
VERSION     NZ_CP135118.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36017835
            Assembly: GCF_032231155.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4440)
  AUTHORS   Zhang,B.
  TITLE     Algicidal process and mechanisms of Enterobacter hormaechei F2
            revealed by an integrated transcriptomic and metabolomic approach
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4440)
  AUTHORS   Zhang,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-SEP-2023) School of environmental science and
            technology, Sun Yat-sen University, Zhonghuan Dong, Guangzhou,
            Guangdong 510000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. 2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 99.48x
            Sequencing Technology             :: Illumina; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/02/2023 13:40:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,546
            CDSs (total)                      :: 4,430
            Genes (coding)                    :: 4,370
            CDSs (with protein)               :: 4,370
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 60
            CDSs (without protein)            :: 60
            Pseudo Genes (ambiguous residues) :: 0 of 60
            Pseudo Genes (frameshifted)       :: 22 of 60
            Pseudo Genes (incomplete)         :: 40 of 60
            Pseudo Genes (internal stop)      :: 8 of 60
            Pseudo Genes (multiple problems)  :: 10 of 60
            Pseudo Genes (short protein)      :: 1 of 60
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP135118.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4440
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="F2"
                     /isolation_source="environmental"
                     /db_xref="taxon:158836"
                     /plasmid="unnamed1"
                     /geo_loc_name="China:Guangzhou"
                     /collection_date="2017-09-10"
     gene            60..383
                     /locus_tag="RPB40_RS22650"
                     /old_locus_tag="RPB40_22650"
     CDS             60..383
                     /locus_tag="RPB40_RS22650"
                     /old_locus_tag="RPB40_22650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001445156.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQVVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            592..>1026
                     /locus_tag="RPB40_RS22655"
                     /old_locus_tag="RPB40_22655"
                     /pseudo=""
     CDS             592..>1026
                     /locus_tag="RPB40_RS22655"
                     /old_locus_tag="RPB40_22655"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1061..1546
                     /locus_tag="RPB40_RS22660"
                     /old_locus_tag="RPB40_22660"
     CDS             1061..1546
                     /locus_tag="RPB40_RS22660"
                     /old_locus_tag="RPB40_22660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_023343076.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMVSQTWLTIVLVSVLLIASNAAILWWQSQQIL
                     DNYVSIREQKSTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            1550..1765
                     /locus_tag="RPB40_RS22665"
                     /old_locus_tag="RPB40_22665"
     CDS             1550..1765
                     /locus_tag="RPB40_RS22665"
                     /old_locus_tag="RPB40_22665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_023343075.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSDQLRRLSSG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            2036..2950
                     /gene="lpxO"
                     /locus_tag="RPB40_RS22670"
                     /old_locus_tag="RPB40_22670"
     CDS             2036..2950
                     /gene="lpxO"
                     /locus_tag="RPB40_RS22670"
                     /old_locus_tag="RPB40_22670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001728425.1"
                     /GO_process="GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A hydroxylase LpxO"
                     /protein_id="WP_023343074.1"
                     /translation="MLSLIIIFVFLISVLYAHSRGKIKQKFSRQLFDHSTFMAPINMFM
                     TGFSKLPSKQAWFPPETFPELQPLIDNWQVIRDEAVNLQGHIKSSEKNNDAGFNTFFKR
                     GWKRFYLKWYGDAHPSAERLCPVTTQLVSSIPCIKAAMFAELPSGSHLGKHRDPYAGSV
                     RYHLGLSTPNDERCFIEVDGVRRSWHDGEVIIFDETFVHWAQNDTEQTRIILFCDIERP
                     MKWRWSQKINRWIGGVLMSAAASPNDNNDRTGAINHLFKYVNAIRDAGQKLKKRNRKLY
                     YLIKNGVISLIFVWLAVSVFRWL"
                     /name="lpxO"
     gene            3350..3811
                     /locus_tag="RPB40_RS22675"
                     /old_locus_tag="RPB40_22675"
     CDS             3350..3811
                     /locus_tag="RPB40_RS22675"
                     /old_locus_tag="RPB40_22675"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_065707752.1"
                     /translation="MVNTYYGYLLGVTLGEWAMGSDVQWQIDNGYLAQVMGDFSTESPS
                     FETPMFGRYFDTFQAAIHVAKAFPGSTLQPSEYEDKTGMLIRHYYVDCHPYYDRLPPEK
                     SDNFQYDEYPSAIHVAACIDQASKELDELLFGGGYGENGEGHASPIILY"
                     /name="hypothetical protein"
     gene            complement(4066..4176)
                     /locus_tag="RPB40_RS22680"
                     /old_locus_tag="RPB40_22680"
     CDS             complement(4066..4176)
                     /locus_tag="RPB40_RS22680"
                     /old_locus_tag="RPB40_22680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048246078.1"
                     /translation="MHDPPVQSDYHAHSGNYQLESNPERHDKIAIGGSHW"
                     /name="hypothetical protein"
     Typing          complement(3850..4360)
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="85.849"
                     /coverage="99.0"
                     /evalue="2.21e-151"
     Typing          374..1863
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.182"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 agatttcccg cccgtcccgg cgttatcagg aggccggatt ggcagacaaa cgcagcaaaa
       61 tgctcacgat gtgggtgacc gaggatgagc accggcgtct gctggagcgc tgcgacggta
      121 aacagcttgc agcctggatg cgccagacgt gcctggacga gaaacccgcc cgcgccggca
      181 agcttccctc gatctcgccg gcgctgcttc gtcagcttgc cggcatgggg aacaacctta
      241 accagattgc ccggcaggtt aacgccggtg gtggcagcgg gcacgaccgc gtgcaggtcg
      301 tcgccgcgct gatggccata gatgccggac tcgagcggct gcggcatgcc gtgctggaaa
      361 agggtgcaga cgatgatcgt taagtttcac ccgcgagggc gcggcggtgg cgccggtccg
      421 gtggattatc tgctggggaa agaccgacag cgcgagggcg ccaccgttct gcaggggaag
      481 ccggaagaag tcagggagct tatcgacgcc tcaccctacg caaaaaagta cacatccggc
      541 gtcctgtcct ttgccgaaaa ggatttaccg cccgggcagc gcgaaaagct gatggcgagc
      601 tttgaacggg ttctgatgcc cggactcgac aaagaccagt acagcgtgct gtgggttgag
      661 caccaggata aggggcggct ggagctgaac ttcctgatcc cgaacacgga gctgctgact
      721 ggcaagcgtc tccagccgta ttacgaccgc gccgaccgtc cgcgcattga tgcctggcag
      781 accatcgtga acggcaggct ggggctgcat gacccgaacg cgcctgaaaa ccggcgggcg
      841 ctggtcacgc cgtccgcgct gccggaaacg aagcaggaag ccgctcaggc aattacacgg
      901 ggtttactgg ccttggcctc gtccggggag ctgaaaaccc gccaggacgt cactgaggcg
      961 ctggaaagcg caggttttga ggtggtacgc accacaaaaa gcagcatcag cattgccgac
     1021 ccggacgggg ggcgaaacat ccgactgaag ggagccatct atgaacagtc ttttaacgct
     1081 ggcgaaggac ttagagcaga aatcgaaagc gcagcagcag agtaccggcg agatgctgaa
     1141 agccgcattc agcgagcacg agaagtctgt cagagcggaa ctgagcgaaa gcgagaagag
     1201 aatcagcgcc gccatcctcg accacgaccg gaagctgtcc tcagccatga gccagcgtac
     1261 gaaagggatg ctgcgcatgg tcagccagac gtggctgacc atcgtcctgg tctccgtgct
     1321 gctgatcgcc tcgaacgcgg ccattctgtg gtggcagagt cagcagatac tcgacaatta
     1381 cgtgagcatc cgggagcaga agagcacgca ggcgatgctg tccgagcgga acagcggcgt
     1441 gcagctctcg acctgcggcg agcagagacg ccgctgcgtg agggtgaacc cggaagcggg
     1501 acggttcgga gaggattcga gctggatgat actggcgggg aaatagcaca tgacagagct
     1561 ggaaaaacag ttgctgagcg cattagagca gctacagcag gactactcga aaaggctgga
     1621 cgagtgggag agcgccttcg cggaatggcg gacgatgtct ggtcttatgc aacgggagaa
     1681 cgcggcgctg agcgagcgcg tcacgggctt gagcaggcag gtgcagagtt tgagcgatca
     1741 gctgcgccgg ttgtcgagcg gctgaatgcc gtcgagctgc agcaggtgcg ggaggcgcgg
     1801 catcagaaag cgctggagct cgaacgatca cagcgacagc gggtatatca cggtccgtcc
     1861 ctttgattca tttcagcata attcctgctg aaatcctctc tgcctgtgta gtagatatgt
     1921 tctggtgaag cttcgattat actattgata aatagcattt agattataat ttaagtgcat
     1981 tcattatgtt atctttaaga gatttttatt attggcgtga acaaggaata tacacatgct
     2041 gtcacttatt attatttttg tttttcttat cagtgtattg tatgcgcatt ctcgcggtaa
     2101 aataaaacaa aagttttcca ggcagttatt tgatcattca acatttatgg ctccgataaa
     2161 tatgtttatg acaggctttt cgaagctgcc gtcaaagcag gcatggtttc ccccagaaac
     2221 atttcctgaa cttcaaccat taattgataa ctggcaggtt ataagagacg aagctgttaa
     2281 tttgcaaggg cacattaaat catctgaaaa aaataatgat gcaggattta atacattttt
     2341 taagcgcggt tggaagcgct tttacctcaa gtggtatgga gatgctcatc catcagcgga
     2401 gcggctttgc ccagtaacaa cccaactggt tagctctatt ccctgtatta aagcagcgat
     2461 gtttgctgaa ttgccttcag gaagccattt aggaaagcat cgtgaccctt atgccggttc
     2521 agttcgttat catttaggat tatcaacacc aaatgatgaa cggtgtttta ttgaagttga
     2581 tggtgtacgc cgcagttggc atgatggtga agtgataatt tttgacgaaa cttttgttca
     2641 ctgggctcag aatgataccg agcagacacg tattattttg ttctgtgata tagaaaggcc
     2701 gatgaagtgg cggtggtcac aaaaaataaa taggtggata ggtggggtgc taatgtcagc
     2761 ggcagcatca cccaacgata ataatgacag gactggtgct ataaatcatt tatttaaata
     2821 tgttaatgct attcgtgatg caggtcagaa gctaaaaaaa aggaatcgaa aactatatta
     2881 tttgattaaa aatggagtta tttctttgat ttttgtttgg cttgctgtct cagtgtttag
     2941 atggctgtga tttgtttttt tctattgagt catacataaa tttgggtctt ctatgctcaa
     3001 tgtatggtta tagataaaaa tcgcctgcta aaaaattcta ttgaaatctt tttagtaaga
     3061 aaggcccgaa tctgacgctc agcgggcctt tcgtgaagaa gtgcggcagt agacagattt
     3121 catctgatca cgttcatgtg ctgctgtgca attaagcgtt tctttcggag ctttcacggt
     3181 atttccttca cgaaaccggc cgccattcac tcttgtgcat agccatgcag ccgcgcagaa
     3241 attatcaggg gcgtttttca gggtttgggc cggggaaagg tgccggaaac ggccctcagg
     3301 ccgcagaaac gcgctgaggg gctctcaggg ggtgcggcca agaagcgtta tggtaaatac
     3361 atattatggt tatttattag gagtaactct gggagagtgg gctatgggat cagatgttca
     3421 atggcagata gataatggtt atttggcgca agttatgggg gatttttcga cagagtcgcc
     3481 tagctttgaa acgccaatgt ttgggcgata ttttgatacc tttcaagctg ctatacatgt
     3541 tgcaaaagct ttcccaggga gtacgttgca gccatcagag tacgaagata aaactgggat
     3601 gctaatacgt cattattatg tggattgtca tccgtattat gatcgactcc ctcctgaaaa
     3661 aagcgataat tttcagtatg atgagtaccc ttctgctatt catgtggctg cgtgtattga
     3721 tcaagctagc aaagaactcg acgaactatt gtttggtggt ggatatgggg aaaatggtga
     3781 ggggcatgca agcccgataa ttttatatta gtgttagggg cggagggata ttatccaatt
     3841 tgtaaaatga tcttcttgag atcgttttgc tctgcgcgta atctcttgct ctgaaaacga
     3901 aaaaaccacc tggggaggtg gtttttcgaa ggttcagtaa gttggggaac ttctgaaccg
     3961 tggtaacagt cttgtgcgag acgtgtcacc aaatctgtcc ttccagtgta gccgtagtcc
     4021 ggccaccact tcaagaactc tcgatacatc tctcgcacat cctgcttacc agtggctacc
     4081 gccaatggcg attttgtcgt gtctttccgg gttggactca agttgatagt taccggaatg
     4141 ggcgtggtag tcggactgaa cgggggggtc gtgcatacag tccagcttgg agcgaactgc
     4201 ctacccggaa ctgagtgtca ggcgtggaat gagacaaacg cggccacata acagcggaat
     4261 gacaccggta aaccgaaagg caggaacagg agagcgcacg agggagctac cagggggaaa
     4321 cgcctgggat ctttatagtc ctgtcgggtt tcgccgccac tgatttgagc gtcatatttc
     4381 gtgatgcttg tcaggggggc ggagcctatg gaaaaacggc aacgccgcat ccttttccca
//
