LOCUS       NZ_CP134665             8146 bp    DNA     circular CON 01-NOV-2023
DEFINITION  Escherichia coli strain 64A:C plasmid unnamed6, complete sequence.
ACCESSION   NZ_CP134665
VERSION     NZ_CP134665.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37298519
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8146)
  AUTHORS   Conquet,G.
  TITLE     Characterization of the Carbapenemase-Producing Enterobacteriaceae
            carriage in a colony of Yellow-legged Gulls (Larus michahellis) from
            south of France
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8146)
  AUTHORS   Conquet,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-SEP-2023) Bacteriology Laboratory, CHU Montpellier,
            191 avenue du Doyen Gaston Giraud, Montpellier, Occitanie 34295,
            France
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 22-FEB-2023
            Assembly Method :: Flye v. 2.9.1; Racon v. 1.5.0; Medaka v. 1.8.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 75x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/01/2023 18:30:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,956
            CDSs (total)                      :: 4,836
            Genes (coding)                    :: 4,709
            CDSs (with protein)               :: 4,709
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 41 of 127
            Pseudo Genes (incomplete)         :: 85 of 127
            Pseudo Genes (internal stop)      :: 26 of 127
            Pseudo Genes (multiple problems)  :: 23 of 127
            Pseudo Genes (short protein)      :: 4 of 127
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP134665.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8146
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="64A:C"
                     /host="Larus michahellis"
                     /db_xref="taxon:562"
                     /plasmid="unnamed6"
                     /geo_loc_name="France"
                     /lat_lon="43.38 N 4.86 E"
                     /collection_date="2022-05-31"
                     /collected_by="Tour du Valat"
                     /genotype="ST1883 (Achtman)"
     gene            complement(join(7918..8146,1..462))
                     /locus_tag="RIK58_RS23610"
                     /old_locus_tag="RIK58_23610"
                     /pseudo=""
     CDS             complement(join(7918..8146,1..462))
                     /locus_tag="RIK58_RS23610"
                     /old_locus_tag="RIK58_23610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            complement(994..1248)
                     /gene="mobC"
                     /locus_tag="RIK58_RS23615"
                     /old_locus_tag="RIK58_23615"
     CDS             complement(994..1248)
                     /gene="mobC"
                     /locus_tag="RIK58_RS23615"
                     /old_locus_tag="RIK58_23615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091747.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobC"
     gene            1427..3586
                     /gene="mobQ"
                     /locus_tag="RIK58_RS23620"
                     /old_locus_tag="RIK58_23620"
     CDS             1427..3586
                     /gene="mobQ"
                     /locus_tag="RIK58_RS23620"
                     /old_locus_tag="RIK58_23620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_001029355.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTELARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLPPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHTTERRHDRGWSQ"
                     /name="mobQ"
     gene            complement(3844..4536)
                     /locus_tag="RIK58_RS23625"
                     /old_locus_tag="RIK58_23625"
     CDS             complement(3844..4536)
                     /locus_tag="RIK58_RS23625"
                     /old_locus_tag="RIK58_23625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281403.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCISRLKSGADAELQKT
                     MTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKNDEKREEFRWIQYRAQYAKG
                     EAKARITFSDAVMPYLTQLKGQFTRVVIKNISGLSSSYSIRIYEFLQQFRSTGERIIAL
                     NDFRSMLGIENKYKQFRDLNKILIKPCVDELNKKSDLAVTVETIKKGRTVVALHFRFKE
                     DKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(5068..5322)
                     /gene="mobC"
                     /locus_tag="RIK58_RS23630"
                     /old_locus_tag="RIK58_23630"
     CDS             complement(5068..5322)
                     /gene="mobC"
                     /locus_tag="RIK58_RS23630"
                     /old_locus_tag="RIK58_23630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091747.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobC"
     gene            5501..7660
                     /gene="mobQ"
                     /locus_tag="RIK58_RS23635"
                     /old_locus_tag="RIK58_23635"
     CDS             5501..7660
                     /gene="mobQ"
                     /locus_tag="RIK58_RS23635"
                     /old_locus_tag="RIK58_23635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_001029355.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTELARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLPPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHTTERRHDRGWSQ"
                     /name="mobQ"
     Typing          complement(1250..1426)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.438"
                     /coverage="100.0"
                     /evalue="2.610000000000001e-86"
     Typing          1427..1785
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.056"
                     /coverage="100.0"
                     /evalue="3.94e-177"
     Typing          complement(5324..5500)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.438"
                     /coverage="100.0"
                     /evalue="2.610000000000001e-86"
     Typing          5501..5859
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.056"
                     /coverage="100.0"
                     /evalue="3.94e-177"
     Typing          complement(2..462)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="85.775"
                     /coverage="100.0"
                     /evalue="1.1e-135"
     Typing          complement(3845..4536)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.965"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(7919..8146)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.478"
                     /coverage="100.0"
                     /evalue="1.15e-90"
     Typing          1341..3583
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.064"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5415..7657
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.064"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gcggaattgc tggaggaact cgtatattct tatactgtaa ctacttgata aacctgatat
       61 gttttttata acaacgcgcg tgaattgccc cttcagttgc gtcagataag gcattaccgc
      121 gtcagaaaag gttattcgag ccttggcctc acctttagcg tactgtgccc gatactggat
      181 ccagcggaac tcctcacgct tctcgtcatt cttgagaatg atcgacctgt cccataaacg
      241 atcgatcgct tcctgtaact gaacctcggc atttttccgc cccatgtcag ggaatgaatc
      301 aaaatattcc gcagcggtaa tggtcattgt cttttgcaat tcagcatcag caccagattt
      361 caatcgactg atacacagca acagaaaacg ttgttcctgt agggtcaact tgtagctggc
      421 ctcaaccaaa gcattcgact tgtaggcaat caagtccgac atacaaagat cccgttcaaa
      481 tgtggtgatg ctttaatgtg caccacaaat aagaaatgtg gaatattttt ttttatatcc
      541 tcacatttct gtgaataaca ccttaaattc ctccaccttt accttaaatt cctccaccta
      601 aacaccttaa attcctccac ctttacctta aattcctcca catataccac tataacatat
      661 tgattttatt aatgaatttc gcccctaaaa caagtattaa aaacaagatc ttaaaaacaa
      721 gatctaaaaa cttaacggcg agcctgtgga taacttgggg gggtgaaaac aaaaaaccaa
      781 taaaaacaat gtgttgcaag aaagcagaat tgcaagttcc gaatactccc cttccaaccc
      841 tgtggaaatc cagtattcat gcgggttcag cgcatatcac ctgccgaaaa accgtgcgta
      901 tgtgttcaac ctggcgacgg attcacttcc gcttcctcgc ccactcgctc gctaacgctc
      961 ggtcgctggg gctgcggcga gcggacgata caatcactgt cctggcagcg cctccagctc
     1021 gttaatcagc ggcagtatgc gcgtcacatc agcctgtcgt gtcacctctg ccttcaggaa
     1081 ctggagtaac caggcacgca actgcacctc ctccggcttt ctgacctttg ccagcaatgc
     1141 tgcacccaca acaaccttct gcgctgtgtc caggctccgg ctcttcgcct tcaggcgctg
     1201 taatctcgcc tcagcatcag caatcttctg ttcgagcgtt ctgctcatga tgtaactccg
     1261 tgcgcgatga aaaatcgcat tttagcgcgt cactggtagt ttaaaaactg agctggcata
     1321 atgcacggca aatcacgaag tgcgcactta tacaatctcc actctgtttc gattgtgtgc
     1381 gccctgcggg gctgaaagaa aactacaaaa aggcattacg gcagaaatgg cgatttatca
     1441 tctcagcatg aaaattatct ccagaagcag cggctacagc gccgtagctt ccgctgccta
     1501 tcgttccggt tcactgatgc ttgatgagcg taccggactg actcacgact acacccgcaa
     1561 aagcggtgtg gctgaagctg tgattctgac ccccgcgaca gcccctgctt ggtgtacgaa
     1621 ccgtgccgaa ctctggaatg ccgttgagaa ggccgaacgc cggaagaact cacaacttgc
     1681 gcgggaaatt gaactcgcta tcccccgtga actgccgcag gatgccgcac gggaaaccgt
     1741 tctcgccttt gtcagagaaa actttgtcag tcagggcatg attgctgatg tggcgttcca
     1801 tcacatggat aaaacaaatc cccatgcgca catcatgctg accacgaggg ctgtcggacc
     1861 tgctgggttc ggcggaaagg tcagggactg gaacgaccgg acacatgccg agacgtggcg
     1921 tgcatcatgg gctgaccatg caaacagagc actggcgaac gccggttatc aggaagagat
     1981 agaccatcgt tcatacgagc gacaggggct tgagaaaacg cctggtattc acctcggaaa
     2041 gtctgcctgt gcgatggaaa cacgcggtat cgaaacagaa cgcggcgaac agaatcgtct
     2101 gattaacagg cttaacctgg aaatacagat ttcccgcact gaactcagga acagcgggct
     2161 gacagagctt gcccgccgtg tggctgacct tctgaatatc gtgcttcctg acaacgcaac
     2221 ttcgtacaca ctacgtgaca tcatcgaagc gttgccgaag gacagtaatg cggcatggaa
     2281 actcaccagt aatgccatgt cgatgatggc agatatgcag gcgacgcgca gacgctggga
     2341 agagctgaac accgaacgaa aggaagcgat gagccatgct gccagcctga aaaaatcatc
     2401 gccgttttcg tccggttttt cccgtattcc cctgatgcaa tgggcggcac cggaataccg
     2461 tagcgaacag ggaaaaatcc agtcgctgag ccagcaaatg gaaaaattgc gacagcatta
     2521 ccggaaggtg gagaaacagg acattcccgc gagtcagacg gcatttgaaa aacagtggca
     2581 gcagtggggg gcatccggcc ttgcagctct cagagtccag ttgcttaaac gtgaagagga
     2641 attgcgccgg aaggagcagg aggatacaga acgtagtcgc gcggagcgac gagcggaaca
     2701 gctcaggaaa aatgacaatg ccgttatgga ttcaggggta ctgagcgcag tcgttacggg
     2761 gtatggcgag gcccccatgc ctggtaacgg ggaaatgacc tgttaccttc tgcttcacaa
     2821 ccggaacgga gaatacacgc tctgggggaa cgagctggag aaataccgca ccagaatctt
     2881 tgagcgtgtt gatttaatgc gtgacaggag tggttatatc tgtgaccgaa gcgaaatcgg
     2941 tcaacatcca ccactgcaac gtgtttattc ctctgcgacc tttgagcaac ttctgacgca
     3001 ggtttgccag acatggccgc aatatacccg caatctccgc cagccgaaga catggccgga
     3061 gtctttctgt cttggagaag accgccagcc agccatgcca tcactggcag caaggaaggt
     3121 ggattttacg cagggacgtc tgccaccgac gctgatgccg gtaatgagtt ctgtcgaccg
     3181 ggaaacccgc cagcttcagc ttttgctggt catgggcgtg gatgactctc tgggcggtgt
     3241 tgtccggctt aatggtacgc tgtatcccgc gtttgccgtt ccctcagccg ataacagcca
     3301 gctggtcatc agtgcactga ccgataaggg gttacggtat gcagggtatg gtgttgccgt
     3361 aaatcacgat gcagacagcc acatatcacc tgcgccggaa cttatggagt ttcatctgaa
     3421 aacccgtgag gcaccgcttt ttgctgccgt caacacacca gaaaaacagc cggatcatct
     3481 gttccgctcg ctgggtttca atcgtacctg ggacgaatgg cggcgcgagg aagatgcaag
     3541 aacacacacc accgagagga gacatgatcg cggttggtca cagtgaatgt gaacaatatt
     3601 ttgttttgtt taacatcgtg ttatgttcgt cgatgttctt cgatgttcat cgacagggat
     3661 gaatgcaaga agcacactca cataacgcaa gcatagtttt gtttttccgc ccccaaaaat
     3721 tgggggttct tttttcgctg catcagagcg tttttgacgc aatctttcac ttcaatagta
     3781 ccttcgcact actaacacaa aaaaaacgca gcagggagcg ttttaggtgc cttaaaatcg
     3841 attttagatc gtcatcttga tctgtttgtc ttccttgaag cgaaaatgca gggctacaac
     3901 cgtgcgcccc tttttgatcg tctcaaccgt tacagccagg tcgctttttt tgttcagttc
     3961 gtcaacacag ggttttatta gtattttatt taagtctctg aattgtttgt atttattttc
     4021 aatccctaac attgaacgaa agtcattaag agcaataatt ctctctcccg tactgcggaa
     4081 ttgctggagg aactcgtata ttcttatact gtaactactt gataaacctg atatgttttt
     4141 tataacaacg cgcgtgaatt gccccttcag ttgcgtcaga taaggcatta ccgcgtcaga
     4201 aaaggttatt cgagccttgg cctcaccttt agcgtactgt gcccgatact ggatccagcg
     4261 gaactcctca cgcttctcgt cattcttgag aatgatcgac ctgtcccata aacgatcgat
     4321 cgcttcctgt aactgaacct cggcattttt ccgccccatg tcagggaatg aatcaaaata
     4381 ttccgcagcg gtaatggtca ttgtcttttg caattcagca tcagcaccag atttcaatcg
     4441 actgatacac agcaacagaa aacgttgttc ctgtagggtc aacttgtagc tggcctcaac
     4501 caaagcattc gacttgtagg caatcaagtc cgacatacaa agatcccgtt caaatgtggt
     4561 gatgctttaa tgtgcaccac aaataagaaa tgtggaatat ttttttttat atcctcacat
     4621 ttctgtgaat aacaccttaa attcctccac ctttacctta aattcctcca cctaaacacc
     4681 ttaaattcct ccacctttac cttaaattcc tccacatata ccactataac atattgattt
     4741 tattaatgaa tttcgcccct aaaacaagta ttaaaaacaa gatcttaaaa acaagatcta
     4801 aaaacttaac ggcgagcctg tggataactt gggggggtga aaacaaaaaa ccaataaaaa
     4861 caatgtgttg caagaaagca gaattgcaag ttccgaatac tccccttcca accctgtgga
     4921 aatccagtat tcatgcgggt tcagcgcata tcacctgccg aaaaaccgtg cgtatgtgtt
     4981 caacctggcg acggattcac ttccgcttcc tcgcccactc gctcgctaac gctcggtcgc
     5041 tggggctgcg gcgagcggac gatacaatca ctgtcctggc agcgcctcca gctcgttaat
     5101 cagcggcagt atgcgcgtca catcagcctg tcgtgtcacc tctgccttca ggaactggag
     5161 taaccaggca cgcaactgca cctcctccgg ctttctgacc tttgccagca atgctgcacc
     5221 cacaacaacc ttctgcgctg tgtccaggct ccggctcttc gccttcaggc gctgtaatct
     5281 cgcctcagca tcagcaatct tctgttcgag cgttctgctc atgatgtaac tccgtgcgcg
     5341 atgaaaaatc gcattttagc gcgtcactgg tagtttaaaa actgagctgg cataatgcac
     5401 ggcaaatcac gaagtgcgca cttatacaat ctccactctg tttcgattgt gtgcgccctg
     5461 cggggctgaa agaaaactac aaaaaggcat tacggcagaa atggcgattt atcatctcag
     5521 catgaaaatt atctccagaa gcagcggcta cagcgccgta gcttccgctg cctatcgttc
     5581 cggttcactg atgcttgatg agcgtaccgg actgactcac gactacaccc gcaaaagcgg
     5641 tgtggctgaa gctgtgattc tgacccccgc gacagcccct gcttggtgta cgaaccgtgc
     5701 cgaactctgg aatgccgttg agaaggccga acgccggaag aactcacaac ttgcgcggga
     5761 aattgaactc gctatccccc gtgaactgcc gcaggatgcc gcacgggaaa ccgttctcgc
     5821 ctttgtcaga gaaaactttg tcagtcaggg catgattgct gatgtggcgt tccatcacat
     5881 ggataaaaca aatccccatg cgcacatcat gctgaccacg agggctgtcg gacctgctgg
     5941 gttcggcgga aaggtcaggg actggaacga ccggacacat gccgagacgt ggcgtgcatc
     6001 atgggctgac catgcaaaca gagcactggc gaacgccggt tatcaggaag agatagacca
     6061 tcgttcatac gagcgacagg ggcttgagaa aacgcctggt attcacctcg gaaagtctgc
     6121 ctgtgcgatg gaaacacgcg gtatcgaaac agaacgcggc gaacagaatc gtctgattaa
     6181 caggcttaac ctggaaatac agatttcccg cactgaactc aggaacagcg ggctgacaga
     6241 gcttgcccgc cgtgtggctg accttctgaa tatcgtgctt cctgacaacg caacttcgta
     6301 cacactacgt gacatcatcg aagcgttgcc gaaggacagt aatgcggcat ggaaactcac
     6361 cagtaatgcc atgtcgatga tggcagatat gcaggcgacg cgcagacgct gggaagagct
     6421 gaacaccgaa cgaaaggaag cgatgagcca tgctgccagc ctgaaaaaat catcgccgtt
     6481 ttcgtccggt ttttcccgta ttcccctgat gcaatgggcg gcaccggaat accgtagcga
     6541 acagggaaaa atccagtcgc tgagccagca aatggaaaaa ttgcgacagc attaccggaa
     6601 ggtggagaaa caggacattc ccgcgagtca gacggcattt gaaaaacagt ggcagcagtg
     6661 gggggcatcc ggccttgcag ctctcagagt ccagttgctt aaacgtgaag aggaattgcg
     6721 ccggaaggag caggaggata cagaacgtag tcgcgcggag cgacgagcgg aacagctcag
     6781 gaaaaatgac aatgccgtta tggattcagg ggtactgagc gcagtcgtta cggggtatgg
     6841 cgaggccccc atgcctggta acggggaaat gacctgttac cttctgcttc acaaccggaa
     6901 cggagaatac acgctctggg ggaacgagct ggagaaatac cgcaccagaa tctttgagcg
     6961 tgttgattta atgcgtgaca ggagtggtta tatctgtgac cgaagcgaaa tcggtcaaca
     7021 tccaccactg caacgtgttt attcctctgc gacctttgag caacttctga cgcaggtttg
     7081 ccagacatgg ccgcaatata cccgcaatct ccgccagccg aagacatggc cggagtcttt
     7141 ctgtcttgga gaagaccgcc agccagccat gccatcactg gcagcaagga aggtggattt
     7201 tacgcaggga cgtctgccac cgacgctgat gccggtaatg agttctgtcg accgggaaac
     7261 ccgccagctt cagcttttgc tggtcatggg cgtggatgac tctctgggcg gtgttgtccg
     7321 gcttaatggt acgctgtatc ccgcgtttgc cgttccctca gccgataaca gccagctggt
     7381 catcagtgca ctgaccgata aggggttacg gtatgcaggg tatggtgttg ccgtaaatca
     7441 cgatgcagac agccacatat cacctgcgcc ggaacttatg gagtttcatc tgaaaacccg
     7501 tgaggcaccg ctttttgctg ccgtcaacac accagaaaaa cagccggatc atctgttccg
     7561 ctcgctgggt ttcaatcgta cctgggacga atggcggcgc gaggaagatg caagaacaca
     7621 caccaccgag aggagacatg atcgcggttg gtcacagtga atgtgaacaa tattttgttt
     7681 tgtttaacat cgtgttatgt tcgtcgatgt tcttcgatgt tcatcgacag ggatgaatgc
     7741 aagaagcaca ctcacataac gcaagcatag ttttgttttt ccgcccccaa aaattggggg
     7801 ttcttttttc gctgcatcag agcgtttttg acgcaatctt tcacttcaat agtaccttcg
     7861 cactactaac acaaaaaaaa cgcagcaggg agcgttttag gtgccttaaa atcgatttta
     7921 gatcgtcatc ttgatctgtt tgtcttcctt gaagcgaaaa tgcagggcta caaccgtgcg
     7981 cccctttttg atcgtctcaa ccgttacagc caggtcgctt tttttgttca gttcgtcaac
     8041 acagggtttt attagtattt tatttaagtc tctgaattgt ttgtatttat tttcaatccc
     8101 taacattgaa cgaaagtcat taagagcaat aattctctct cccgta
//
