LOCUS       NZ_CP134361            13231 bp    DNA     circular CON 22-SEP-2023
DEFINITION  Escherichia coli strain TUM13936 plasmid pMTY13936_3, complete
            sequence.
ACCESSION   NZ_CP134361
VERSION     NZ_CP134361.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37323662
            Assembly: GCF_031661555.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13231)
  AUTHORS   Aoki,K.
  TITLE     Visualization of food-borne spreading blaCTX-M trafficking to human
            using blaCTX-M-8 as an indicator: blaCTX-M-8-carrying IncI-1/pST113
            plasmid was transferring host from chicken E. coli to human E. coli
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13231)
  AUTHORS   Aoki,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2023) Department of Microbiology and Infectious
            Diseases, Toho University, 5-21-16, Omori-nishi, Ota-ku, Tokyo
            143-8540, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.5.0
            Assembly Name                     :: TUM13936
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 142x
            Sequencing Technology             :: Illumina MiSeq; PacBio RS
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/22/2023 09:09:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,846
            CDSs (total)                      :: 4,731
            Genes (coding)                    :: 4,616
            CDSs (with protein)               :: 4,616
            Genes (RNA)                       :: 115
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 49 of 115
            Pseudo Genes (incomplete)         :: 65 of 115
            Pseudo Genes (internal stop)      :: 14 of 115
            Pseudo Genes (multiple problems)  :: 14 of 115
            Pseudo Genes (short protein)      :: 3 of 115
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP134361.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13231
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="TUM13936"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pMTY13936_3"
                     /geo_loc_name="Japan"
                     /collection_date="2013"
                     /collected_by="Toho University"
     gene            join(13161..13231,1..307)
                     /locus_tag="RJ646_RS24185"
                     /old_locus_tag="RJ646_24185"
     CDS             join(13161..13231,1..307)
                     /locus_tag="RJ646_RS24185"
                     /old_locus_tag="RJ646_24185"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="WP_310984186.1"
                     /translation="MRIRPDICALPCIGKYSRPLRHRKQGTNFVVNGSLILAFFYLVVI
                     GTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDWLGTLLILSSV
                     ILISMDSRRRARKINRPARHE"
                     /name="EamA family transporter"
     gene            complement(311..1129)
                     /gene="nlpA"
                     /locus_tag="RJ646_RS24190"
                     /old_locus_tag="RJ646_24190"
     CDS             complement(311..1129)
                     /gene="nlpA"
                     /locus_tag="RJ646_RS24190"
                     /old_locus_tag="RJ646_24190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="WP_032281859.1"
                     /translation="MKLTTHHLRAGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQD
                     VAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGYKL
                     VAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKEGKG
                     LLPTALDITENPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDSVFIE
                     DKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
                     /name="nlpA"
     gene            1338..1631
                     /gene="yicS"
                     /locus_tag="RJ646_RS24195"
                     /old_locus_tag="RJ646_24195"
     CDS             1338..1631
                     /gene="yicS"
                     /locus_tag="RJ646_RS24195"
                     /old_locus_tag="RJ646_24195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="WP_001626280.1"
                     /translation="MKLTMLLMITVFLTFPAISQAESPFSSLQSAKEKTTVLQDLRKIC
                     TPQASLSDEAWEKLMLSDESNKQHIREAIVAMERNNQNNYWEALGKVECPDM"
                     /name="yicS"
     gene            1933..2429
                     /locus_tag="RJ646_RS24200"
                     /old_locus_tag="RJ646_24200"
                     /pseudo=""
     CDS             1933..2429
                     /locus_tag="RJ646_RS24200"
                     /old_locus_tag="RJ646_24200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604188.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone"
                     /name="SycD/LcrH family type III secretion system
                     chaperone"
     gene            2439..2621
                     /locus_tag="RJ646_RS24205"
                     /old_locus_tag="RJ646_24205"
     CDS             2439..2621
                     /locus_tag="RJ646_RS24205"
                     /old_locus_tag="RJ646_24205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000700143.1"
                     /translation="MKINGKNASFTPEELARKYHEEAPVDLKNNHGDKINEKNNNKHIT
                     YFPVDAILDENKHLP"
                     /name="hypothetical protein"
     gene            2676..4458
                     /locus_tag="RJ646_RS24210"
                     /old_locus_tag="RJ646_24210"
                     /pseudo=""
     CDS             2676..4458
                     /locus_tag="RJ646_RS24210"
                     /old_locus_tag="RJ646_24210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001600713.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="type III cell invasion protein SipB"
                     /name="type III cell invasion protein SipB"
     gene            4471..5622
                     /locus_tag="RJ646_RS24215"
                     /old_locus_tag="RJ646_24215"
     CDS             4471..5622
                     /locus_tag="RJ646_RS24215"
                     /old_locus_tag="RJ646_24215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001626282.1"
                     /translation="MATYEIKNATSTLNINNLDKKIFDRDYSKESNISNDNKIVQYYNL
                     KDIKHDISSQFIILKNTDREVCPELRVKLLDNLNYSRNTLDNSKSDVDKATITSSQSSC
                     STEQTTIKISKFNILDMLDNLTMRQQVVMEEFLRVFTSMYKASAMSEAIMSNVFEQLVQ
                     YQRKETERATAASSVGVIIGSGVGAVGATASAVANYRSHPEKMVTLTPDQEHTINRTDD
                     DVQRASKREVREQQASLKQIDEKIKNTTKDTSLNEEQKEEALKSYDKEKETTLEKIRDT
                     NEKISTKHNNRYNKYFTIGKADVLSQLSNESIRATSRFLESTTHAEKDIVEQAKNVMHS
                     VMSSYLDQINRDVQGRDQALNLLQQIIQTIIDTDSAIVQGIRS"
                     /name="hypothetical protein"
     gene            5665..7011
                     /locus_tag="RJ646_RS24220"
                     /old_locus_tag="RJ646_24220"
     CDS             5665..7011
                     /locus_tag="RJ646_RS24220"
                     /old_locus_tag="RJ646_24220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IpaD/SipD/SspD family type III secretion system
                     needle tip protein"
                     /protein_id="WP_310984187.1"
                     /translation="MINKDNNLNIDILRQQNANIPLKEASSQSEEPHQLGNSKSEGNLH
                     NSASKDNNIGAMLKKDVEKLSTLVSERSPRNEVWKCQHDIQLNFVKNMLRNFPTENLSE
                     KGSELLHESHLLIKNVLQLTENINTIDGKLKDPAKILTLHNAVRKTLANVADNTTGSSA
                     MATTKGTLTHAIASLDSSVPEMRSSATSSALTASSSSEFKSDKYICSDIADLMSVLGSD
                     YLEIYANSVEIMSAYWQDFSEHIQSNMGKWTHSNKKGDAIVFDVNAFQKALMHFYYIDK
                     YPNGDFHYHYNPDYVLYPPAPADKIGVPLEEAEKWCSALGLPVIPPDPKHRTPSPIVEV
                     EPQGSGLYVIIPNPQIIDSMSQSSDSMVHRDDKGKEKNISKEFTGYEISTAEYQAWLAG
                     YNSQAENMKTDVQVITTKYSTANSTYDTIIKLLSSTITALFDSAKDLRF"
                     /name="IpaD/SipD/SspD family type III secretion system
                     needle tip protein"
     gene            7214..8862
                     /gene="eilA"
                     /locus_tag="RJ646_RS24225"
                     /old_locus_tag="RJ646_24225"
                     /pseudo=""
     CDS             7214..8862
                     /gene="eilA"
                     /locus_tag="RJ646_RS24225"
                     /old_locus_tag="RJ646_24225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176839.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="HilA family transcriptional regulator EilA"
                     /name="eilA"
     gene            complement(8914..>13151)
                     /locus_tag="RJ646_RS24230"
                     /old_locus_tag="RJ646_24230"
                     /pseudo=""
     CDS             complement(8914..>13151)
                     /locus_tag="RJ646_RS24230"
                     /old_locus_tag="RJ646_24230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907437.1"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /name="Ig-like domain-containing protein"
ORIGIN
        1 acaaggaaca aactttgtcg ttaacggcag tttgattctg gcgttttttt atttggtggt
       61 cattggtaca tctctgacat ttagcctgta cctgaaagga gcacaattaa ttggcggtcc
      121 aaaagccagc attttaagct gtgcagaacc attaagtagc gcgctgctct ctttgctgtt
      181 gctggggatc acgttcacat tgccggactg gctgggaacg ctgctgattc tgtcatcggt
      241 aattttgatt tcaatggatt cccgtcgtcg cgccagaaaa ataaatcgtc cagcgcggca
      301 tgagtgagaa ttaccagcca ggcaccgcgc caccgttaaa gatggtttct gccgcttttg
      361 cgacttcggg tgattgataa gattgcagaa attccttcac gttttctgca ttcttattat
      421 cttcccgtgc caccaaaata ttcacatacg gcgaattttt atcttcaatg aatacgctgt
      481 cgtgcaccgg agaaagcccg gtctgctgaa tgtaagtggt gctgataatc gccacatcaa
      541 ctttgggatc atccagtacg cgcgggagct gcgccccctc gagttccata atctgcaaat
      601 gacgaggatt ctcggttata tccagcgcgg taggtagtaa cccttttccc tctttcagag
      661 taattagctt ctctttttgc aacagtaaaa gcgcgcgacc aaggttggtc ggatcgttgg
      721 ggatcgccac tgtcgcgcct tcttttattt gcgccacggt ttttattttt ttggaataac
      781 ccgccatggg gaagacaaac gtatttccca ccgctaccag tttataacca tgcgcctgat
      841 tatcttgttc aagaaagggt cgatgctgga agacgttcgc atcaagctcg ccatgatttg
      901 tcgcatcgtt cggtagcaac gaaccgctaa aacctaccag ttcaacatcg aggccatatt
      961 tctcttttgc caccttttta gcgacttccg cgacatcttg ttctgcgcca tttataacgc
     1021 caactttaat gtgttttgca tcgctgctgc tctggtcgca acctgccagc agaattcctg
     1081 ccagcaataa tgcggccccc gcccgtagat gatgtgttgt cagtttcatt ttttatcctt
     1141 tttttaatca ttaactgact ataatggcaa gataactacg atttaaaaaa cgaaaagcta
     1201 tcgaatagaa gaaaaaggta taaaaaactg gcacttctta ctgaccacgc aaacaccttt
     1261 cgtacagcat gatgagagcg gcaaaagcaa actacactta tgcttcattc gatgcacatt
     1321 tgcagaaggt tgccgctatg aagctaacga tgctactcat gattaccgta ttcctgactt
     1381 ttccagccat ttctcaggcc gagtcgccat tcagttcgct acagtcggca aaagagaaaa
     1441 ccacggtatt gcaagattta cgtaaaattt gcacgccaca ggcgtcatta tcagacgaag
     1501 cgtgggaaaa attaatgttg tctgatgaga gtaacaaaca acatatccgt gaagctattg
     1561 tggcgatgga gcgtaataat cagaataact actgggaagc gctgggtaag gtggaatgcc
     1621 cggatatgta aatttcgcac acctccgcgc tcaggccagc agccgctgac ttaagctcgc
     1681 acatcagtaa atcacctgct ttttcaaatt ccaatcaaaa ttgaaccagt tcatcaggat
     1741 tgtggataaa tttccctcta cattattttc atttcatcac atagaaaatc ttatctcatt
     1801 gaaacaatac ttgtttttaa tgcagaaaac gaagcccaac ggatgggcat aaaaatgagc
     1861 gaattttgat atcaccaccc tctaaaaaat ggtgaagtct gagcaaagaa tgttcgaaag
     1921 gagagagtag agatggaaag cgcaaatctg gatttagaag aaaataaaga aattgccagt
     1981 aagttcgaac gtgcactagg tatgggagca acacttgcag agctgcatgg cattactcgg
     2041 atacccttga aggcgtttat gcttacgctt ataactttta tgaaaaagga agacttgacg
     2101 aagctgaact cttttttaaa tttctctgta tttatgattt ccaaaattat aactacttaa
     2161 aaggatatgc cgccgtttgt cagttgaaga aagattatca aaaggcattc gacatgtatc
     2221 atatctgtct aatgctatct cccgataacg atttctcttt ggtttattat atgggccaat
     2281 gccagatggg cttaaaaaat attaaaatgg ccacggagtt atttaataca gtcgtaacct
     2341 acagtcaaaa tgaaaaagtt aaagagatgg ctactgctta tcttgaactt ttgacagcca
     2401 acaccgaaga ggaagttcaa acagagtgag tcagcaaaat gaagataaac ggaaaaaacg
     2461 cttcttttac gcctgaagag ctagccagaa aatatcatga ggaagccccg gttgatttaa
     2521 aaaataacca tggcgataaa ataaatgaga aaaataacaa taaacacatt acttactttc
     2581 ccgttgatgc cattttagat gagaataaac atctgccatg agttaaataa aaagaattaa
     2641 ttacacccgg tgatagctta aaaaggaggt gaattatgtc ggcaccgatt acgggacaaa
     2701 cgatcacgtt tgaacaaatc agcgaaacat tgcgtacaca gtatagcgat gcggaaaaaa
     2761 gactgcagga cagcagcaaa acgcaagttg atcccatgcg gcttaataaa aacccgaaat
     2821 cccttgataa cgatattcgc gcgctcgtct tgaaaacaaa ccgatgcttg ctccgcctgc
     2881 aatacaggtt tcagattcag acaacgccac tacagcaaag acgaatgatg cccgcctgac
     2941 aatgattttg ggtaatttaa caggcattgc cgatcaggat attacaacac gattgcataa
     3001 taaccttgaa agcaccctgc ttcgacacga aatggcacat aataagtttc gtgaattaag
     3061 tgacgcttac tcctcatcat tagatgatgc gcaaaaagcc gacgatatca tgcatcaggc
     3121 caataatatt acaatgcggt cgataaaaag gtgcaatcgc tggagaaaaa agtcaacaca
     3181 ttgaatcaag agttgtcaca actgcaacct ggcgatccgc aatataataa agtactgacg
     3241 caaaaaaatg cggctgaaaa aacactcacg ctgtcattac aaaaaaaatc gttagctgag
     3301 aaatcgttaa atacagccat tatggatgct gatgctgcga tcggccaaag tatggaaatt
     3361 tttgacgaaa ttcaacagca agaacagatt aataacttca ccaccaatat ttgcctgacc
     3421 caggaaaacc agaaaaacag aaacgcgaca gccacattta tcctttttga ttacctcagt
     3481 aatggaagtt attggcgata ccaattgcga ctctattaaa aatcaatctg aggtaatgaa
     3541 agagattaac catgtcaggg aaaataaact caatgaaaca gcgcgtaaat atactaccac
     3601 gactaaagtt ttaaaaatcg ttaatgagtg tgtaactgta gctaccattg ccatgagtgc
     3661 aatcttaatt gttgttggtc ttcttgctgc agttcctagt ggtggttcaa gtattgcggg
     3721 tgcactggcg cttattggtg gaatagcggg cgcagtggta ttaggtgttg atatcacttg
     3781 ccaaatcgct ctggtaccac tgctaccggc tggattttgg aaaaagttgt tgaaggtctt
     3841 tcggccgcta ttaaaacagt tgatcccact ctactcgcaa tcaccgcgct tctggacgtt
     3901 attggtgtcg atcaagatac gattgaactg gttaaaagta tttacgcaag tgcagccgct
     3961 tctcttatca tggcgacagt aatgattggt gcagctgtaa tatgttctgt agcaataggg
     4021 gccgttgttt ctgcattatc aaaaacagcc gcagaagaag tcaccaaaga aataacaggc
     4081 actataaaat caaccattga atccattatt aattccgttt caaaaaatat tataaaggta
     4141 ttagacagcg tatgcagcat gatacaaaca tcagtcgtag ttttgaagtt aattgccaag
     4201 ataagtaatg gcctggagaa aataggctta cttatctgtg aaacagcaac atcgataatg
     4261 aattattgtg ttgctgaaaa ctctgccgac atggcaattt tacaacagga catgagtaat
     4321 ctttcaaaaa cgcgtgaaca aatgctttca gtattgcaaa gggtggataa aaccgtcgaa
     4381 caagaggtaa gccagatggt acgagtatta caacaccgaa ctgaagcctt aaaatttgct
     4441 tcccattcta tcgtataaga ggattttaac atggccacct atgaaattaa aaatgcgacc
     4501 agcacactta atattaacaa tctcgataaa aaaatctttg atagagatta tagcaaagaa
     4561 agcaacatat caaatgataa taaaattgtg caatactata accttaaaga tattaagcac
     4621 gacataagct ctcagtttat aatattaaaa aatacagaca gagaagtctg tccggaacta
     4681 agagtaaaac tattggataa tcttaattac tcaaggaata cactagataa ttcgaagagc
     4741 gacgttgaca aagctacaat tacatccagt caatcaagct gttctacaga acagaccacc
     4801 attaaaatat ctaaatttaa tatccttgat atgctagata atttgaccat gcgacaacag
     4861 gttgtaatgg aggaattttt aagggtattc acatcaatgt ataaagcctc tgctatgtct
     4921 gaggctatta tgagtaatgt ttttgaacaa cttgtccagt atcagagaaa agaaaccgaa
     4981 cgcgccacag cagcaagttc ggtaggtgtt attatcgggt caggagttgg cgcagtcggg
     5041 gccacagcat ctgccgtagc aaattacaga agccatcccg aaaagatggt aacccttaca
     5101 cctgaccaag agcatactat caatagaact gatgatgatg tacagcgcgc atcaaaaagg
     5161 gaagttcgag agcaacaagc ttctctcaaa caaatagatg aaaaaataaa aaatacaacg
     5221 aaagatacga gtcttaatga agaacaaaaa gaagaggcgc tgaaatctta tgataaagaa
     5281 aaagaaacta ctctagaaaa gattagagat acaaacgaga aaattagtac aaaacataac
     5341 aataggtata ataaatattt tactataggt aaagcagatg tactttcaca gcttagcaat
     5401 gaatcaattc gagcaacatc tagatttcta gaaagcacta ctcatgcaga aaaagacatt
     5461 gttgagcagg ctaaaaatgt tatgcattca gttatgagta gctatctgga tcaaattaat
     5521 cgtgatgttc aaggaagaga tcaggcactc aatttattac agcaaataat acaaaccatt
     5581 attgatactg atagtgctat cgttcaaggc attcgctcat aaataattgc aataatagtt
     5641 aattatatta aggaggaatg acacatgatt aataaagata ataatttaaa tatagatatt
     5701 ttacgccagc agaatgcaaa cattcctctt aaagaggcat cctcgcaaag tgaggagcct
     5761 catcaactgg gaaacagcaa aagcgaggga aatttgcata acagcgccag taaggataat
     5821 aatattggcg ctatgctcaa gaaagatgtt gagaaactat caactttagt cagtgaacgt
     5881 agcccgcgaa atgaagtctg gaaatgccag catgacatac aacttaactt cgttaaaaac
     5941 atgctcagaa atttcccaac tgagaacctg tcagagaaag gatccgagct cttacacgaa
     6001 agtcatttgc tgataaaaaa cgttctgcag cttacagaaa atattaatac tattgacgga
     6061 aaattaaagg atcccgctaa aatattgacc ctgcataacg ctgtgagaaa aaccctggct
     6121 aacgttgctg acaacacaac cgggtcaagt gcaatggcaa cgaccaaagg aaccttaact
     6181 cacgctatcg cttctttaga tagttctgtg cctgaaatga gaagttcagc aaccagcagt
     6241 gctttaactg ccagtagttc aagcgaattt aaaagtgata aatatatatg cagtgatatt
     6301 gctgatttga tgagtgtgct cggtagtgac tacctcgaaa tctatgcaaa tagcgttgag
     6361 ataatgagcg catactggca ggacttcagt gaacatatcc aaagcaatat gggaaaatgg
     6421 actcactcta ataaaaaagg cgatgcgata gtttttgatg tcaatgcatt tcaaaaggcc
     6481 ttgatgcatt tttattatat cgataaatat cctaatggtg atttccacta tcattacaat
     6541 cccgactatg ttttataccc tccggcacca gctgacaaaa ttggtgttcc actggaagaa
     6601 gcagaaaaat ggtgctccgc tttaggactt ccggtaattc cacccgatcc caaacaccgc
     6661 acaccttctc cgattgtcga agttgaaccg caaggtagcg gtctgtatgt tattattccc
     6721 aaccctcaga ttatagacag tatgagtcag tcttcagact caatggtaca cagggatgat
     6781 aaagggaagg aaaaaaatat atctaaagaa tttaccggct acgaaataag cacggctgaa
     6841 taccaggcat ggctggcggg ttataacagc caggcagaaa acatgaaaac cgacgtgcag
     6901 gttataacga ctaaatacag tacagccaac tccacctacg atacaattat aaaattatta
     6961 tcttcaacca ttactgcgtt gtttgattca gcgaaagatt tacgtttctg attaagtaac
     7021 acctacaatt atcgtccact atctggtccc gtaaaatggg accagcgttt catattatta
     7081 agcatcacct ttcattcatt ttttcatcac aatcttacaa aatgagcgtt ttttgataga
     7141 gcaattctgc caatacttta ttttagacag atcaaattaa tactatgaat agagaaaatg
     7201 gaaggagcgt tctatgcaac tgcaacatgg aaaaaataat acacaacaat atatatttgg
     7261 cgactttgta ttaaaaaaca atggaatatt attattcaaa aataaagaat atcatattcc
     7321 ccccgaaaga acttggcgtt atcattctat tacttaatgc tgacggtgaa attgttagca
     7381 agattatcga taaggtctgg agcgcgagtt gctagtgatg aatctttaac acggtgtatt
     7441 tatgcattac gtaaactttt gcatgagaat aaacagtgta agtatatcga aacggtctat
     7501 ggtcgtggat accggtttgt aaccgataat gaaccggtaa agtcgaccac tcttgccgtt
     7561 tttccctttc gtactgaagg ttcgatcaat gttctaaaac tccattacga actggtccag
     7621 ggcctttcaa aatatgcttt ttgtggttta gatattttac ccgcttcggt aaccaatgag
     7681 gccagtgatt tctccactat ccatcagttt atcaataaaa caggtccaga atattatatt
     7741 atgggtcagg tcgttcacta tggtaaaaac tggcgtttat ttattgagtt aatccatgcc
     7801 agaacgcata aattgattga tcatcaaagt attgatttca accccgacaa tcctctttcc
     7861 gtgctattat cacaagtaat taatattctt attgaaaaaa tacccaacat taattttaaa
     7921 tccattaata cacaacaaat gccatctctg gattctgctg ttatgtatat gaatggcagg
     7981 atggaaatgt accgttatac accagaaagt ttgaaacgag cattagtaat tgagattgtg
     8041 tgagtacgca accgcaaaat accttgccct actgttgcct ggcggaatgc catatatcat
     8101 tagcactcct tggaatcagt gaacaaaaac aagcaataac tacggcagta acctccattg
     8161 aaaaggctct ggaaattaac ccttcaaatt ctcaggcatt gggtttactg ggtttaatca
     8221 gtgggctaaa agatgaacat tcagtgtcta atgtgctatt taaacaagca catttactca
     8281 aacctaattc gccagacgtt tattattatc agtctctgct atgctttttg aatggtgacc
     8341 ttgcaagagc atttaatcta atagaaaaaa gcattgctct tgaacccaat aaaatgggca
     8401 taagcattct taaattaatt atactttatt acacatcccc cctcgataac gcgatatctt
     8461 ttgcattaaa tattaacagt caaaaccctg taacaatcca attattatat ctattctggc
     8521 tatgttcatg gcactgaaag gacataatga caaagccaaa agtttatttt gaacctgaac
     8581 atggtcttga ttacacctgc gttaactctt tatacgaagt ttctgatcta tggcgcatct
     8641 ataaaaaatg acattatgaa gttactggca aatattaaca ctaacaaaat caatggtgtt
     8701 attttgccgt taatttacac tgtttatggt aaaaaagaat atgaagcgca acaattaatt
     8761 aaagagaatg atttgtggtc aaatgttttg cttcatgacc cccgtttgct cagtgtgaaa
     8821 aatgaattta atactattgg tgtgatgcgt acttctgcgt aagaactaat ataaattgcc
     8881 ctgctgatgc agggcaattt agtgattact tcgttaataa tactctacac acaagccaga
     8941 agactttcct gtcccaagct ctgatatacc agtttcagga ttaagtaccc ctaagagctg
     9001 agtggttgtc caataatccg taccgctaaa agcgttccca taagttttca aattaccata
     9061 ttcgccccac aacgtaccta cttttcgcgg tactacccca ccgcttggcc gctgcgctaa
     9121 ttctaaatga cggcatttga ccatagctgc atgaagcatt tatgatatcc caggtattac
     9181 tactggcagc acctaaacta gtatttttaa tcttgaattt aaatacaaca tcattttatt
     9241 cttgggtttc ccggtaatag taacttcact tcttattagc agagataatg ttattactgt
     9301 taattaattc cataaatccc agccgtccaa ttatatccgt atttctcata ttatgggtta
     9361 gtaacagcgt gaattttgca ccatcaaacc ctgtttctgg aaaaccatct gtcgccctga
     9421 atcttacacc attaacactc agcgactcga cttcgacaaa aggtaggatg gtataagacg
     9481 gttgcccgtc tacataccaa ccattaatat gtagatatga gtttaagttt tctccttctt
     9541 tgtaggccct ataggtacgc tcatatctgc cttcacccac agatacccat tagccccttc
     9601 tttcttttcc acattttgta agtacacggc atcctctgtc agcaattcca gaccgtcatt
     9661 cacaggatta ccaaattcat cagataataa acaggtatat ccgccccagc taccactaaa
     9721 acttcgcgta tacgaagctg tgaacgaaac atacttactt cacctctgtt aatagtgtag
     9781 ttttttgaat aattggcatc gctccaacca gccatcttca gttgcgcatt caaattcgct
     9841 tccgcaaaat gcgccatata ctgtctttga tatataccat tgccattgta aacccaatta
     9901 ttgccctgga taggatccgc attcctgact tgtacgtttt cctcattaag ctgaacaacg
     9961 ccatcagtaa taaagttatc ctgggcatct ttaagaacaa cggtcaccgt tatgctatcc
    10021 ccagcaatat atatcgtttt gtccgcgaca atcgtggatt gagtttttgc cgccgcacct
    10081 ggttgtattc cgtataacga ggacttaatc tccgttgccc atgtttttaa tttcagactg
    10141 gcaaccagat tttcctcggc aacccatgct gtccagacaa tggtatattc accattattc
    10201 tgctcgaccc actgcataga atcggaagtt gcgccaccta ccgcgaaatt atcgatataa
    10261 gactcgatga cttctgatgt cagccctaaa gcagggttac caaactcatc ccgcaacgtt
    10321 attgtgacag ttaatggctc cccggcgata tatgccgatt tatctgtcct gatagcagaa
    10381 tttgcctgca ccggagcacc actgtggatg gaataactct ctgattgttt actgctgccc
    10441 tcgggcaacg tcagcgtcgc gtggtgagag tctcccgcca tttgcgccga ccatgtagca
    10501 gtataaacac cggcagtttc agaccatcct cctgaacgta cggctccttc tactgttacg
    10561 ttatcaccag acagtaagga ctcgccccct tcaaccaggt taccgtgcgc gtctttcagc
    10621 gtcacggcga cggttatagt ctcgccagcg gtgtatgcgt tttttatctg tggcgatagt
    10681 tgatgtggct tgcgccggag aaccggactg tatgtcatag cgatctgacg ttacaccatc
    10741 actccacgtc tgcagttgca ggttagcgtg ctgatcggta ccggcaatcg tcgccattcg
    10801 ggttgtggta tacacgccac ctgattcaac ccagcttccc gtttctgacg ggtcggtgcc
    10861 atccaccgtc agcacactat tagcggctaa ctgtgaagct gcaccggtta ccagattatc
    10921 gaacgcatcc cggagcgtta ccgtgatggt gatggtctct ccaaccgtgt agttagactt
    10981 atttgtttca acggttgaag tgagctgcga tgcgtcaccc ggtacaaagg tgacgttctg
    11041 gctcaggcta tgattgttga tggttgctgt gactgcgcta actcccgccg tctgactggt
    11101 gacactgaac tccaccgtac cgtccgactg cgtggtgacc gtcggagtga ccgttgcgcg
    11161 ctgttgcctg ccgtcacgct gaccgtctgc ccggcaagca cattgccgtt agcatcggta
    11221 acctttacct gcagcatgtt cgccattgcg ccgtcagcca ctgagccgtc cttaatgacc
    11281 accagatccg cgatcttcgc cgtcctgaca tcggcaacaa acgtcacgtt ccggctcgcg
    11341 ttgctgctgt tgatactggc ggtgactgca ctgactcccg ccgtctggct ggtgacactg
    11401 atctccgccg tgccgtccgg ctctgtgatc accgtcgggg caaccgttgc gccgttgcct
    11461 gccgtcacgc tgaccgtctg tccgccaagc gcattgccga acgcatcggt gaccttcacc
    11521 tgcagcgtgt tcgccattgc gccgtctgcc actgagccgt ccttaatgac caccagatcc
    11581 gcgatcttcg ccgtcctgac atcggcaaca aacgtcacgt tccggctcgc gttgctgctg
    11641 ttaatgctgg cagtgactgt actgatcccc gccgtctggc tggtgacact gatttccacc
    11701 gtgccgtccg gctgcgtggt gaccgtcgga gctgtcgtgg caccgttgtc cgccaacacg
    11761 ctgaccgtct gcccggcaag cacattgccg ttagcatcgg tgaccttcac ctgcagcgtg
    11821 ttcgccattg cgccgtctgc cactgagccg tccttaatga ccaccagatc cgcgatcttc
    11881 gccgtcctga catcggcgat aaacgtcaca tcccggctct ggctgctgct gttgatgctg
    11941 gcggtgactg cactgactcc cgccgtctgg ctggtgacac tgatctccgc cgtgccgtcc
    12001 ggctctgtga tcaccgtcgg ggcaaccgtt gcgccgttgc ctgccgtcac gctgaccgtc
    12061 tgtccgccaa gcgcattgcc gaacgcatcg gtgaccctcg cccgcagcat atccgccatt
    12121 gcgccgtcag ccatggcgtt atcctgcgtc acctccagac tcgcgatctg cgccgttctg
    12181 acatcggcga taaacgtcac atcccggctc tggctgctgc tgttgatgct ggcagtgact
    12241 gtactgatcc ccgccgtctg actggtgaca ctgatctcca ccgtgccatc cggctgcgtg
    12301 gtcaccgtcg gagtgaccat tgcgctgttg tctgtcgata cgctgaccgt ctgcccattc
    12361 agcgcattcc cgaatgcatc ggtgaccttc acttgcagcg tattcgccat tgcaccgtct
    12421 gccactgagt tatcccgggt gactaccaga tcagcgatct tcgccgtcct gacatccgca
    12481 ataaacgtga cgttccggct ctggctgctg ctgttaatgt tggcagtgac cgcagtggtg
    12541 cctgccgtct ggctggtgac agggatctcc accgtgccgt ccggctgcgt ggtgaccgtc
    12601 ggagtgaccg ttgcgccgtt gcctgccgtc acgctgaccg tctgtccatt cagcgcatta
    12661 ccgaacgcat cggtcaccct tgcccgcagc atattcgcca ttgcgccgtc agccactgag
    12721 ccgtcctgca tcaccaccag atcagcgatc ttcgccgtcc tgacatcggc aacaaacgtc
    12781 acgttccggc tcaggctgct gttgttgata ctggcggtga ccgcactgac tcccgccgtc
    12841 tggctggtaa cagagatctc caccgtcccg tccggctcgg tgataaccgt cggagcgacc
    12901 gttgcgctgt tgtccgccaa cacgctgacc gtctgcccgt ccagcgcatt accgaacgca
    12961 tccgtcaccc tcacccgcag cgtgttcgcc gtcgaaccgt ctgccactga gccatcctta
    13021 atgaccacca gatcagcgat ctgcgccgtg ctgacatcag caacaaagtt caccgtttta
    13081 ctatcgctgc tttgattgac cttagcctca acagtgctgg catccgctac ggtactgctc
    13141 agatcaacct ctgccatacc atgcgcatcc gtcctgatat ctgcgctctg ccctgcattg
    13201 gcaaatacag ccgccccctt cgtcaccgta a
//
