LOCUS       NZ_CP134045            10046 bp    DNA     circular CON 21-OCT-2023
DEFINITION  Klebsiella pneumoniae strain SCKP170089 plasmid unnamed5, complete
            sequence.
ACCESSION   NZ_CP134045
VERSION     NZ_CP134045.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37176446
            Assembly: GCF_032833975.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10046)
  AUTHORS   Lai,Y.-C.
  TITLE     Diversification of ST11 KL64 Klebsiella pneumoniae in Taiwan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10046)
  AUTHORS   Tang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) Microbiology and Immunology, China Medical
            University, No. 91, Xueshi Road, Taichung 40402, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/21/2023 13:05:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,805
            CDSs (total)                      :: 5,679
            Genes (coding)                    :: 5,575
            CDSs (with protein)               :: 5,575
            Genes (RNA)                       :: 126
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 47 of 104
            Pseudo Genes (incomplete)         :: 53 of 104
            Pseudo Genes (internal stop)      :: 14 of 104
            Pseudo Genes (multiple problems)  :: 10 of 104
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP134045.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10046
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="SCKP170089"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed5"
                     /geo_loc_name="Taiwan: Changhua"
                     /lat_lon="24.24 N 120.54 E"
                     /collection_date="2017-07-23"
                     /collected_by="Show Chwan Memorial Hospital"
     gene            join(8719..10046,1..634)
                     /locus_tag="REM96_RS28975"
                     /old_locus_tag="REM96_28975"
     CDS             join(8719..10046,1..634)
                     /locus_tag="REM96_RS28975"
                     /old_locus_tag="REM96_28975"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_044704830.1"
                     /translation="MLNTDSLAWQWHIPELARQIYPWVSWLYREYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNDRAAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLVLLGGLVVAVFIESWGETVLRPGYYANADYYLLQPANLFGIAWCAGGLV
                     VGILLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARQQQVVFLGKDEDGQPVRVPREAWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLAETGDVADHYRISEQKAAKLIAEQFPHGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRAWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVDRREIPLKTFPVLAKTDPLAEY
                     QAEAVRQRPGDDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            634..1365
                     /locus_tag="REM96_RS28980"
                     /old_locus_tag="REM96_28980"
     CDS             634..1365
                     /locus_tag="REM96_RS28980"
                     /old_locus_tag="REM96_28980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="WP_008321412.1"
                     /translation="MALEQYNVSHAKRQARNAEKTRLVLRWLRDELCSTAETVARLLGI
                     GAVQPVYRFLDSLVAKGLLARAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLDMEARGATGWRTLHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="replication-relaxation family protein"
     gene            1372..1902
                     /locus_tag="REM96_RS28985"
                     /old_locus_tag="REM96_28985"
     CDS             1372..1902
                     /locus_tag="REM96_RS28985"
                     /old_locus_tag="REM96_28985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_044704832.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMAVMAWQTGAYVFRFE
                     DQAQHLAMMKFIGMYHERLNSDDKATYYFTGKNPAWERDKPSGMKLCDYLYRKVEQFAI
                     ENGVQVLDAGKYTPEQIGAMIDVVSERHGESAVNEKLKAELMREYNSRYFNEPVPVLPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(1930..2109)
                     /locus_tag="REM96_RS28990"
                     /old_locus_tag="REM96_28990"
     CDS             complement(1930..2109)
                     /locus_tag="REM96_RS28990"
                     /old_locus_tag="REM96_28990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032934837.1"
                     /translation="MTKQEKTALNMAKFIRDQSLLLLEKLNELDMDDPADMCERLHEDA
                     EKLWLVMRGKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2179..2556
                     /locus_tag="REM96_RS28995"
                     /old_locus_tag="REM96_28995"
     CDS             2179..2556
                     /locus_tag="REM96_RS28995"
                     /old_locus_tag="REM96_28995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_008321415.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQGETHERDGLRLPDETEKTASEALIAEIKALREEVAGLRQEMQAMRLLEH
                     KPETVETPAETPRRWWHWGKR"
                     /name="hypothetical protein"
     gene            complement(2618..2917)
                     /locus_tag="REM96_RS29000"
                     /old_locus_tag="REM96_29000"
     CDS             complement(2618..2917)
                     /locus_tag="REM96_RS29000"
                     /old_locus_tag="REM96_29000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010848978.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_009365129.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYEVDDPAQKVRVLAVVGQRQNPHTVR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2907..3170)
                     /locus_tag="REM96_RS29005"
                     /old_locus_tag="REM96_29005"
     CDS             complement(2907..3170)
                     /locus_tag="REM96_RS29005"
                     /old_locus_tag="REM96_29005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000072676.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            3943..4461
                     /locus_tag="REM96_RS29010"
                     /old_locus_tag="REM96_29010"
     CDS             3943..4461
                     /locus_tag="REM96_RS29010"
                     /old_locus_tag="REM96_29010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_020956924.1"
                     /translation="MQKVTDLYPPEIARHKLQNHFAGNTVMLELIQKLNKTSLCTFAAL
                     CDGNVVTTSGYNITADLCVNRASAVAHSLKQKYLPVTARTISTKADVGGAVKQAAFCID
                     EDDLARLKSEPEKMMKECERNLNSQKRTNAQKEMSRLYKEFGEDGILALLRNVAQSNGT
                     PPPGAHPAS"
                     /name="hypothetical protein"
     gene            5046..6734
                     /locus_tag="REM96_RS29015"
                     /old_locus_tag="REM96_29015"
     CDS             5046..6734
                     /locus_tag="REM96_RS29015"
                     /old_locus_tag="REM96_29015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_020956933.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGVKDYGHDYHPAPKTEEIKGLGELKKAPKKTPKQGGGGRRDRWIGDKGRKVY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQIKGPDPKGRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            6744..7001
                     /locus_tag="REM96_RS29020"
                     /old_locus_tag="REM96_29020"
     CDS             6744..7001
                     /locus_tag="REM96_RS29020"
                     /old_locus_tag="REM96_29020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WP_041937905.1"
                     /translation="MGLKLNLTWFDKKTEEFIGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGGFDVEKPWVPLLQPYFKNKIEVDKYWYQISFDYRDGNW"
                     /name="cloacin immunity family protein"
     gene            7086..7235
                     /locus_tag="REM96_RS29025"
                     /old_locus_tag="REM96_29025"
     CDS             7086..7235
                     /locus_tag="REM96_RS29025"
                     /old_locus_tag="REM96_29025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_020956931.1"
                     /translation="MKKVKVVFLFILIFLGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     BGC             46..10046
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(7339..7917)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.454"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          635..1362
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.239"
                     /coverage="100.0"
                     /evalue="1.14e-141"
ORIGIN
        1 gaaaatgctg tttgcccgct gcgcgcaaat catcattgcg cgggacgagt tccgcgcgtg
       61 gccgcacgtc agcgtgatgc tcgatgaaat caaatatctg ctctcgaagt acgtactcaa
      121 cgcgctgggg acgctgcgca gccgggactg taacctgctg ctggctcacc agtcgctggg
      181 cgacttcggc cagtgcgggc aggatctgcc cgctgatttt gtgaaaacca cggtactgga
      241 taacacgcca atccgctggt tctatcgcgc ggccagccag gagtccgcgc agtgggcggc
      301 cggacagacc ggggaaatcc gggtggacgt ggagcgccgc cgggccagcc gggaggcggg
      361 gaacgtggag cacatcagcg gggacagctt catccagaag gaagcgcggc cgctgttcga
      421 cgtgaacacc ctgcaacacc tgccggacgg gttcgcggtc atgaccgggc tgggcgtggc
      481 gcggctggcg ttcagcagcc cgctgcgggt tgatcgccgg gaaattccgc tgaaaacctt
      541 cccggtgctg gcgaaaacgg acccgctggc ggagtaccag gctgaggcag tgcggcagcg
      601 cccgggtgac gatgattttg cggggctgta ctgatggctc tggagcaata caacgtcagc
      661 cacgcgaagc gacaggcgcg gaacgcggag aaaacccggc tagtcctgcg gtggctgcgg
      721 gacgaactct gctcgacggc tgaaaccgta gcccgtctgc tggggatcgg ggcggtgcag
      781 ccggtttacc gctttctgga cagcctggtg gcgaaaggac tgctggcccg ggcgaaatac
      841 ccggttgacg gtcgtcaggt cagcgtgtgg gggctgaccc cgcacggcgt ggcgttctcg
      901 ttcgacgagg acgagccgct gacggacgtt attccgtttc agccctcgcg ggtgtcggcg
      961 gcgcagctgc cgcaccggct ggccgtgcag tccctgcgtc ttgatatgga agccagaggg
     1021 gccaccggct ggcgcaccct gcaccgcctg agtcttaaag gtatgaaggt gccggatgcg
     1081 ctggcagagc tggacggtaa aacggtggcg tttgaggtgg agcgcaccgt caagagccgt
     1141 cgccggtatc aggaagtggt gtccgggtat ctgttcaacc gtaaggcgaa cggcatcgat
     1201 gaaatctggt acatctgccc cgaccggccg acgcttgtcc gggtacagcg ggcgattctg
     1261 gcggtggatg aaatcgtgaa cccgcagacc ggcgaagccc gcaaaacggc ggaactggac
     1321 cgggagaggc tgttcgcgtg ctttaaattt atgacaacag agtgagggat tatggcgctg
     1381 tacatggttt ttattgagga tgtggaagac aaagacggcg agcgctgcat tatgccaacc
     1441 agcgagcatc tggaagacat ggcggtcatg gcctggcaga ccggggcata tgtattccgg
     1501 tttgaagatc aggcccagca tctggcgatg atgaagttta tcggcatgta tcacgaacgg
     1561 ctgaacagcg atgacaaagc cacgtattac ttcaccggca aaaatccggc gtgggaacgt
     1621 gataaaccgt ccggaatgaa gctgtgtgat tatctttacc gcaaggttga gcagttcgcc
     1681 atcgagaacg gcgtgcaggt actggacgca gggaagtaca ccccggagca gatcggcgcg
     1741 atgattgacg tggtgagtga gcggcacggt gagagtgcgg tgaacgagaa gctgaaagcg
     1801 gagctgatgc gagagtataa cagccggtat tttaacgagc cggtgcccgt attgccggag
     1861 gataaacccg cccgccggtg gtggccgttc ggcaaaaaat aaacgccagc accggccatg
     1921 ccggtgctgt tattcttccc cgagcttacc ccgcattacc agccagagtt tttccgcatc
     1981 ctcgtgaagt cgttcgcaca tgtcggccgg gtcgtccatg tccagctcgt tcagcttttc
     2041 cagcaacagc agcgactgat ccctgatgaa ttttgccatg ttgagggcgg ttttttcctg
     2101 cttcgtcatg attttttccc ttccgggcat ggtgtgcgcc agtcgggtat actgtctcaa
     2161 aaatagcggg gtgagacaat ggcgtgggtg acggtgagac aggcgacgga actgacgggg
     2221 aaagcccgga gttccctgta ccgggatatg gcgaaaggcc gcgtatcata ccggacggag
     2281 gctgatggcg gtcgggttgt ggataccagc gagcttatcc gggtgtacgg tgagttaaga
     2341 cagggtgaga ctcacgagcg ggacgggttg agactgccag atgagactga aaaaacggcc
     2401 tctgaagcgt tgatcgcgga gataaaagca ctcagggagg aggtggccgg gttgcggcag
     2461 gaaatgcagg ccatgagact gctggaacac aaaccggaga ccgtagagac accggccgaa
     2521 acgccgcgcc ggtggtggca ttggggaaaa cgctgacgcg ctgcgcttgt ccaaccggcg
     2581 acgggcacgc aatgaattgc gcccctgtca ccggttatta gcgaacggta tggggattct
     2641 ggcgctggcc gaccacggcc agaacccgga ctttctgcgc ggggtcgtcc acttcataga
     2701 ggacatgttg tcccatcagg ctgatccggc gaacgtcttc gccataacgt gagtccactt
     2761 cgtagaggcg cgggaagtcc gccagtttgt ccgcagcagc gagaacccgg tcggtaacgt
     2821 tctgcgcatc ccacagcgat gaattttccg cgatatagtc aaacagtgtc gttacatctt
     2881 cgatggctcc ctgcttccat tccacgtcat acggcatcgc gcagttcctg ataacggcca
     2941 tgcaggcgct ggcggagttg ttccgtggtc atgcccggac gcgtgtcagc acgggcggcg
     3001 gtcactttgg cgcgaacctg atcttcatac tctgcctggg ccagttcttc gttagccagg
     3061 cgctgcaaca gcgcagcttt gtccgggcca ccttccggca gtatgcgggg caggctttca
     3121 gccagttccc gaagaaagtt ggcgttttcg tagtgtgtcg tgttgctcat tgcggagcct
     3181 cccgggttaa tgcggcaata tgaaaaagtt tagcaggagg cggcctgaaa gaatatgctc
     3241 gtgtcagtca ggctgatgaa gcagaaagtc ggtaaaaaag ggcttacggc atccatttta
     3301 cgtcaaatcc catgccatgc agttacgtgc atagccatgc gttcaggtac aatttatccg
     3361 gcgcgtttcc gggggtgttg cgggtggttt gttgtctgtt ttatcggttt cccgtcagaa
     3421 atgccctcag aggctctcag ggggtgcggg caacggatgt tatggtaaat ggctggttat
     3481 gaggcattca gctgacgatt tttgcgtcaa cttttcgctg tatgtttaaa catgagttta
     3541 acattagcgt catctgatag cgctgttgct aatttcatcg accagacagg taatttcctt
     3601 gtaaatcaag cgcgaaagat ctgcaaaaaa gctatgtgtg cgcttatgga ataaggtaga
     3661 ataagggatt ctgatgatcg tgaggttgtc agtgaaaaac gaggcagcta tcagatcttc
     3721 ggcctgagaa actaaggatt ctgatagctg ccggtgactc agttcgtgaa aactgaatct
     3781 tacctgatca acaaacgccc gattagggcg tttgtcaagt ggtgctgcat tatgtagtaa
     3841 cttggtaccg acttgccagg catttttcac agttttttga tgtgcgaatg ctcatccgga
     3901 caccgtcacc tgttatttat cctctgtaaa caggtgctat aaatgcaaaa agtaactgat
     3961 ctgtatcctc ctgaaattgc tcgccataag ctccagaatc acttcgccgg taataccgtt
     4021 atgctggagt taatccagaa actaaataaa accagcctgt gtacttttgc ggcgctctgt
     4081 gacggcaacg tagtgaccac ttccggctat aacatcacgg ccgatctatg tgttaaccgt
     4141 gcgtcagccg tggcccattc gcttaagcaa aaatatctgc cagttacggc cagaacgatt
     4201 tcgacaaagg ctgatgtagg tggtgcggta aagcaggcgg ctttctgtat cgatgaggac
     4261 gatcttgcaa ggctgaaatc agagccggaa aagatgatga aagagtgcga acgtaacctg
     4321 aacagccaga agcgcaccaa tgctcagaag gaaatgagcc ggttgtacaa ggagtttggt
     4381 gaagacggca tactggcgct gttacgcaat gttgcccagt cgaacggtac accgccgcca
     4441 ggcgcacacc ctgcaagttg ataccagtaa agcccctaag gggctttatt tttaaggttt
     4501 tctggatgcc gccagctctt gatctgtcag ctaatcgcgg tgcagatagt tcccttctac
     4561 cggcggtggc gttaccagtt ttgcttttcc ttctttcgga cgagaatttc actcttaccg
     4621 tacattttcg tacggttgga cctcgaaccc ctttcaggcc catggccagt gcctgagcga
     4681 caaaggcgtc cgccaggcgc ttttcttcag gtgcgtcatg gtggcctccg ggttgtctcc
     4741 gattcctgaa cagtttaacc gcaggtactc tcctcatgcc atttcgcttt caggtacatc
     4801 tataccaata taaaagtaag tgtgatgaat atctcaaata ataaaatttg ttgccatgag
     4861 aaattttcgg gaaaagacgc gatctgctta caggcggctc cgggcgcttt tttgtgcctg
     4921 aagatgagaa ctaatctcat atattgacac ctgaaaactg gaggagtaag gtaacaatta
     4981 cactgtgtat atatacagta tattggtttt aatttattgt tttaaatgtc aaagaggaaa
     5041 acgatatgag tggtggagac ggtcgaggtc cgggtaattc aggtctggga cataatggtg
     5101 gtcaggccag tgggaatgtg aacggtacgt ctggtaaagg tggcccttca tcaggtggtg
     5161 gtacggatcc aaacagcggg ccgggctggg gtacgacgca tacgccgaat ggagatattc
     5221 ataactataa tccgggggag tttggtaacg gcgggagtaa acccggtggt aatggtggta
     5281 acagcggcaa tcatagcggt agctctggtg gcggacagtc ttcggccact gcgatggctt
     5341 tcggtctgcc tgccctggct actccggggg ctgaaggact ggctttatca gtttccggcg
     5401 atgcgttgtc ggccgccgtt gctgatgtgc tggctgccct gaaggggccg tttaagtttg
     5461 gtctgtgggg gattgcgatc tacggtgtgc tgccttctga gattgcaaaa gatgatccga
     5521 aaatgatgtc aaaaattatg acgtcattac cggccgatgc ggtgacggag actccggcaa
     5581 gtactttacc actggaccag gcgacggttc gtgtcagaca acgggttgtg gatgtggtga
     5641 aggatgagcg gcagcatatt gcggttgtcg caggtcggcc aatgagtgtc cctgtggtgg
     5701 atgcgaaacc gacaaaacgt ccgggggtat tcagtgtgtc gattccgggt ctcccgtctc
     5761 tgcaggtgag cgtaccgaaa ggtgttccga cagcgaaagc cccgccaaaa ggcattgttg
     5821 ctgaaaaagg tgattcacgt ccggctggtt ttacagccgg tggtaactcc cgtgaggccg
     5881 ttattcgttt cccgaaagag acaggacaga agccggttta tgtgtcggtg acagatgttc
     5941 ttaccccggc acaggtaaaa cagcgtcagg aggaagaaaa gcgtcgccag caggcatggg
     6001 acgccgctca tccggaagag gggctgaaaa gagactatga taaagcgaaa gctgagctgg
     6061 atgccgaaga taaaaatatt gcgaccttaa acagccgcat tgcatcgaca gagaaggcgc
     6121 tccccggtgc aagggctgct gttcaggaag ccgataaaaa ggtaaaagag gcagaggcaa
     6181 ataaagatga ttttgtgact tataacccgc ctcatgaata tggctccggg tggcaggatc
     6241 aggttcgcta tcttgataag gatattcaga atcagaatga gaaattaaaa gcggctcagg
     6301 catctttaaa cgcaatgaat gaatccttat ccagagataa ggctgcactt accggggcga
     6361 tggagagccg gaaacaaaag gagaaaaaag cgaaggatgc agaaaataag ttaaatgagg
     6421 aaaagaacaa gcctcgcaag ggagttaaag attacggtca tgattatcat cccgctccga
     6481 agaccgaaga aatcaagggg ttgggggaat taaaaaaagc acctaaaaaa acacctaaac
     6541 aaggtggtgg tggtagacgt gaccgttgga ttggtgataa aggccgtaag gtttatgaat
     6601 gggactccca gcacggtgag cttgaagggt atcgtgccag tgatggcgaa cacctcgggg
     6661 catttgatcc aaaaacgggt aagcaaatta aaggtccgga tccgaaagga cgaaacatta
     6721 aaaaatatct ttgagaggta agtatggggc ttaaattaaa tttaacctgg tttgataaga
     6781 agactgagga atttattggt gaggagtatt ctaaagactt tggtgatgat ggttctgtca
     6841 ttgaaagcct tggaatgcct ttaaaggata atattaacaa tggcgggttt gatgttgaga
     6901 aaccatgggt tccgttgttg cagccatatt ttaaaaataa aattgaagtc gataagtact
     6961 ggtatcaaat ttcatttgat tatcgggacg gtaactggta acaaaaaatt atcaaggatg
     7021 atttgaaata cagacttccg gtgttcaggg atgaatgccg gggtttttaa atacagggaa
     7081 ggtatatgaa aaaggtaaag gttgtttttc tttttatatt gattttttta ggctttcttc
     7141 tggtcgcatg tcaggcaaac tatatccggg atgttcaggg tggaacggtg gcaccatcgt
     7201 cctcctctga actgacgggg atcgcggttc agtagaaaag atcaaaggat cttcttgaga
     7261 tccttttttt ctgcgcgtaa tcttttgccc tgtaaacgaa aaaaccacct ggggaggtgg
     7321 tttgatcgaa ggttaagtca gttggggaac tgcttaaccg tggtaactgg ctttcgcaga
     7381 gcacagcaac caaatctgtc cttccagtgt agccggactt tggtgcacac ttcaagagca
     7441 accgcgtgtt tagctaaaca aatcctctgc gaactcccag ttaccaatgg ctgctgccag
     7501 tggcgttttg cgtgcttttc cgggttggac tcaagtgaac agttaccgga taaggcgcag
     7561 cagtcgggct gaacggggag ttcttgctta cagcccagct tggagcgaac gacctacacc
     7621 gagccgagat accagtgtgt gagctatgag aaagcgccac acttcccgca agggagaaag
     7681 gcggacaggt atccggtaaa cggcagggtc ggaacaggag agcgcaagag ggagcgacct
     7741 accggaaacg gtggggatct ttaagtcctg tcgggtttcg cccgtactgt cagattcatg
     7801 gttgagcctc acggctccca cagatgcacc gaaaaagcgt ctgtttatgt gaactctggc
     7861 aggagggcgg agcctatgga aaaacgccac cggcgcggcc ctgccgtttt gcctcacatg
     7921 ttagtcccct gcttatccac ggaatctgtg ggtaactttg tatgtgtccg cagcgcccgc
     7981 cgcagtctca cgcccggagc gtagcgaccg agtgagcgag gaggcgcaat ttccccggca
     8041 cctgcaagac ggtcaccgga cgcgctgatg tgggatattg tcccctgatt aagtctttct
     8101 attccctgac gatcaattat ccgaataggt gaaccatgtc ggtttttcgc tgaacatttt
     8161 aagttaacct atctctgttg cttttttctc cttgcggaga aatgcaagtc atccccgctg
     8221 gggcgtggct gacttcaggg ggaaatcccc tgcttacgct ggctggacgc caccgacgca
     8281 cggtctttcc ccctctttct ggctctgtcc ggctggacgc cggtcacgcc agaaattcac
     8341 ccggcccccg tcaggctcac gcctgaccac ccacaaaaat ccacggtgct ggacgcacct
     8401 gacgaagcag caaagctgct cctgatttta tgggtggtca gccgctcgct gtcgcttact
     8461 ctgacggggg ccggaacacc ggaaaagaca gcactggggt gccgcctttt cctctcgcac
     8521 gctgtgggcg tgctcgttgt tcctccgggg ctgcgccttt tccgcatggg gcggaaagag
     8581 gctggcctcc gtggcgagcg ctgaacgctt tcaccacgga cgtgaacgcc gccgccatgc
     8641 cttggggcat gtcgtcagct tgcgcagatg ggctgcgcat cacgccggta aatccgtcgt
     8701 ctgtaggaga gaggttgcat gttgaatacg gattcgcttg cctggcagtg gcatatcccg
     8761 gagctggccc ggcagattta cccgtgggtc agctggctgt accgggagta ttacggtctg
     8821 gccggggctt ttctggttat cggcctgttt ttcggtctgg ctgaactctg ggccggacgg
     8881 atgcgttttg tgggcctgag ccataaggag cggcgggagt atgccgatcg ctataagcgc
     8941 gataatgaca gggctgcgcc attttcggtg ctggcctcgc tggattctcc cgggcggatg
     9001 gtcagggctc gtcgacatgt ctggaactac gcgtactggc tgaccggcgc tgccggtctg
     9061 gttctgctgg gggggctggt tgtcgccgtg tttatcgaga gctggggaga aacggtgttg
     9121 cgtccgggct actacgcgaa tgccgattat tacctgctgc aacccgctaa tctgttcggg
     9181 atagcctggt gtgccggtgg tctggtcgtc gggatacttc tggcgcggct actggcattc
     9241 ctgctgcttg acggtcattt tgcggccgct gacgaggcgg tcaatgcaaa actgaatcag
     9301 gagagccggc gcagcagcca gcgcaccggc gaaatgactg acgtgcgcca cctgcatttc
     9361 ggggagccgg tgccggtcaa cgcgctggcc gatttttcca cggagcaggc ccggcagcag
     9421 caggtggtgt tcctgggtaa ggacgaggac ggccagccgg tgcgggttcc gcgcgaggcg
     9481 tggcgcaaga ccaatattca gatcctcggc ctacccggga gcggcaagag cgtgatggcc
     9541 accaacgcgc tgatccgctg cgtgcgcgat tttggcgatg cggtggtgta cttcgacccg
     9601 aaaggcgacg catgggcgcc acacgtcttt cgggcgcact gcccggactt caccctgctg
     9661 gacctgcggc cggggaaacc ggcgcagctg aacctgttcc gcgaccttga ccagtacgca
     9721 ctgaaaaatc tgctggttgc cgggtttaac ctggcggaaa ccggcgatgt ggccgaccac
     9781 taccgcatca gcgagcagaa agcggcgaag cttatcgcgg agcagttccc gcacggcgcg
     9841 aacattcagc aggtgctggc ggcggcgtat gcgctgccgg aggcgctgaa aaaggacgtg
     9901 aaggggctga tcaccaagct ggagaacgtc gcggacctga gcgtcctgca gacggattcc
     9961 ggtattgatg tggcggggat tatcaacggc ggcggctgtc tgtacgtcat cggatcgatg
    10021 gacgacgagg cggtgatccg ggtgca
//
