LOCUS       NZ_CP134040             4938 bp    DNA     circular CON 21-OCT-2023
DEFINITION  Klebsiella pneumoniae strain CMUH20001 plasmid unnamed5, complete
            sequence.
ACCESSION   NZ_CP134040
VERSION     NZ_CP134040.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37176142
            Assembly: GCF_032833155.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4938)
  AUTHORS   Lai,Y.-C.
  TITLE     Diversification of ST11 KL64 Klebsiella pneumoniae in Taiwan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4938)
  AUTHORS   Tang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) Microbiology and Immunology, China Medical
            University, No. 91, Xueshi Road, Taichung 40402, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/21/2023 13:12:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,846
            CDSs (total)                      :: 5,722
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 110
            CDSs (without protein)            :: 110
            Pseudo Genes (ambiguous residues) :: 0 of 110
            Pseudo Genes (frameshifted)       :: 49 of 110
            Pseudo Genes (incomplete)         :: 59 of 110
            Pseudo Genes (internal stop)      :: 12 of 110
            Pseudo Genes (multiple problems)  :: 10 of 110
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP134040.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4938
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="CMUH20001"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed5"
                     /geo_loc_name="Taiwan: Taichung"
                     /lat_lon="24.24 N 120.54 E"
                     /collection_date="2019-12-05"
                     /collected_by="China Medical University Hospital"
     gene            510..623
                     /locus_tag="REP97_RS29200"
                     /old_locus_tag="REP97_29200"
     CDS             510..623
                     /locus_tag="REP97_RS29200"
                     /old_locus_tag="REP97_29200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032634168.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHAKAPLAAATGN"
                     /name="hypothetical protein"
     gene            890..1495
                     /locus_tag="REP97_RS29205"
                     /old_locus_tag="REP97_29205"
     CDS             890..1495
                     /locus_tag="REP97_RS29205"
                     /old_locus_tag="REP97_29205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006338602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_047715645.1"
                     /translation="MQDRSVVNRQGFNPEIDLPYSLRISDFEHAMEDIYDFFHDVNNLL
                     SNKGLHRLDDMMRPAAMSGLISDMITASLAKFSRSLVENKHFNGHPDLVVRGRYPNDSI
                     SSGEEGIEIKTTRKNGGAVDTHGARSQWMCVFVYNVDNETEPASDRKSMKFTEVYLCHV
                     DENDFRRNDRGALGTRTATLHKEGLKKLRESWVYKDLV"
                     /name="hypothetical protein"
     gene            complement(1492..2610)
                     /locus_tag="REP97_RS29210"
                     /old_locus_tag="REP97_29210"
     CDS             complement(1492..2610)
                     /locus_tag="REP97_RS29210"
                     /old_locus_tag="REP97_29210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012402750.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0008170 - N-methyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0006306 - DNA methylation [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="WP_117064210.1"
                     /translation="MSLAPGVVRDSIIECFLRLGREASIEEITKAVYLKIGEISKSSVR
                     SYLSLNTPGLFERVDRGVYRLNGDAGITPSLDLTSSPKTVGKSTIFFDDAIEWLKRQKD
                     NSIHAVVTDPPYGLVEYTKQQTEKLRAGKGGIWRKPPSFDGHKRAPLPRFTVLREEDLR
                     NISLFFKELSSQVERVVVPGGNIVIASNPLLSHIVADSMSTSGLELRGYIVRQVMTMRG
                     GDRPKNAHDEFGDVSVMPRSQWEPWIVLRKPLDGRVQDNLRKWKTGGFRRISADRPFGD
                     VIKSHPTPAHEKKIAPHPSLKPQNFMRQLVRAVLPLGEGIILDPFMGGGSTLAAANHIG
                     YESIGVEIDREFFDLAEKAIPALSALPSKENN"
                     /name="DNA methyltransferase"
     gene            complement(3086..3301)
                     /locus_tag="REP97_RS29215"
                     /old_locus_tag="REP97_29215"
     CDS             complement(3086..3301)
                     /locus_tag="REP97_RS29215"
                     /old_locus_tag="REP97_29215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_064147933.1"
                     /translation="MTELEKQLLSALEQLQQDYSRRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVSGLSRQVQSLSEQLRRLSTG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3308..3805)
                     /locus_tag="REP97_RS29220"
                     /old_locus_tag="REP97_29220"
     CDS             complement(3308..3805)
                     /locus_tag="REP97_RS29220"
                     /old_locus_tag="REP97_29220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_015328238.1"
                     /translation="MSSLLTASRDFEEKSKAQQQLTEERLKAAFSKHENVVKSELNASA
                     KRINDAISAHEQGMNAAMQSNRLSVLRMVGRTWLTITMVSGLLFASLSGVLWYQGNRIA
                     ANLAEIRQQRDTLSKLHMQTWGVTYLENRNGRFLVLPEGMKAETGRTVDNKTRNAVKLV
                     RE"
                     /name="MbeB family mobilization protein"
     gene            complement(<3846..4544)
                     /locus_tag="REP97_RS29225"
                     /old_locus_tag="REP97_29225"
                     /pseudo=""
     CDS             complement(<3846..4544)
                     /locus_tag="REP97_RS29225"
                     /old_locus_tag="REP97_29225"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(4483..4824)
                     /locus_tag="REP97_RS29230"
                     /old_locus_tag="REP97_29230"
     CDS             complement(4483..4824)
                     /locus_tag="REP97_RS29230"
                     /old_locus_tag="REP97_29230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_074181101.1"
                     /translation="MADKRNKMLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPTR
                     AGRLPSISPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAV
                     QEKGPDDDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          104..623
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.587"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(2971..4493)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.803"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4490..4803)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.509"
                     /coverage="99.0"
                     /evalue="8.420000000000001e-58"
ORIGIN
        1 accagtcacg tagcgctagc ggagtgtata ctggcttact atagtggcat cggtatgggt
       61 atcagcgaaa gtgctatata tggcagggat gacatgcagc gacggtgcgt aaggcagata
      121 atggtagtgc cgggggtatg acgcctcctc gctcactcgg tcgctgcgct cctgccgtga
      181 gactgcggcg ggcgttacca gctcacgagt gacgagagaa agaaatagcg ctcacgtcag
      241 gaaggccaga aaaaaggatg cggcgttgcc gtttttccat aggctccgcc cccctgacga
      301 gcatcacaga atctgatgct caaatcagtg gtggcgaaac ccgacaggac tataaagatc
      361 ccaggcgttt ccccctggta gctccctcgt gcgctctcct gttcctgcct ttcggtttac
      421 cggtgtcatt ccgctgttat ggccgcgttt gtctcattcc acgcctgaca ctcagttccg
      481 ggtaggcagt tcgctccaag ctggactgta tgcacgaacc ccccgttcag tccgactgct
      541 gcgccttatc cggtaactat cgtcttgagt ccaacccgga aagacatgca aaagcgccac
      601 tggcagcagc cactggtaac tgaaaaggtg gatttagata ttgagagttc ttgaagtggt
      661 gacctgactg cggctacact agaaggacag tatttggtat ctgcgctcca ccaagccagt
      721 tacctggtta agcagttccc caactgactt aaccttcgat taaaccgcct ccccaggcgg
      781 ttttttcgtt tcaagaggct acgcccaaac gacaatgatc gtcaaggatc ttcttggtaa
      841 gatatgacaa agagctttcg aacttttata tcttattcaa ggaactaaga tgcaagacag
      901 gtcagtagtt aatcgacagg gatttaaccc agagatcgat ctgccgtatt cgctaaggat
      961 tagtgatttt gagcacgcga tggaagatat ttatgacttc ttccatgatg tgaacaactt
     1021 actatcaaac aaaggtttgc ataggcttga tgacatgatg agaccagcag ctatgtctgg
     1081 cctcatatca gatatgatca cagcttccct cgctaaattt tcaagatcgc ttgttgaaaa
     1141 caagcatttc aatggacatc cagacctagt tgttaggggc aggtatccga atgacagtat
     1201 ctcctcagga gaagaaggca ttgaaataaa aaccacccgg aaaaatgggg gggcagtcga
     1261 tactcacggg gccagatctc aatggatgtg cgtatttgtc tacaatgtcg ataatgagac
     1321 cgaaccggcc tcagacagaa agtcgatgaa atttaccgag gtatatcttt gccacgttga
     1381 tgaaaatgac ttccgtcgta atgatcgtgg tgcattagga accagaacag caacccttca
     1441 caaagaaggg ttgaagaagc tcagagaaag ctgggtttac aaggacttag tttagttatt
     1501 ttccttgcta ggaagagctg atagcgcggg gatagccttc tccgccaaat caaagaactc
     1561 tcggtctatt tcaactccaa tgctctcata acctatatga tttgcagctg ccaatgtgga
     1621 gcctcccccc ataaatggat cgagaataat cccctctccc aaaggaagca ctgccctcac
     1681 taattgtcgc atgaaatttt gcggctttaa tgagggatgt ggtgctatct ttttttcgtg
     1741 tgctggcgtc ggatgagatt tgatgacgtc gccaaatggc ctatcagctg aaatcctcct
     1801 gaatccacct gttttccact ttcttagatt atcctgaacc cttccatcta gaggctttct
     1861 caacactatc catggttccc attgagaacg aggcatgaca gaaacatctc caaattcatc
     1921 atgggcgttt ttgggtctat ccccacctct catggtcata acctgacgta caatgtaacc
     1981 tcgtagctct aagccagacg tagacataga gtccgcgact atatgggata gtagagggtt
     2041 ggatgcgata actatattcc cgccaggaac aaccaccctt tcgacttgcg aggatagttc
     2101 tttgaaaaat aatgatatat ttctgaggtc ttcttcccgc aaaaccgtaa agcgaggtaa
     2161 tggcgctcgc ttatgtccat caaaggaagg tggctttctc caaataccac ctttccctgc
     2221 ccttagtttc tcagtctgtt gttttgtata ctcgactaat ccgtaaggag gatcagttac
     2281 aactgcatga attgaattat ctttttgtct ttttaaccat tcaattgcat cgtcaaaaaa
     2341 aatcgtcgat ttcccaaccg tcttaggtga agacgtcaaa tctagggagg gagtaatacc
     2401 agcatcacca tttaagcgat agacacctcg gtctactcgc tcgaacagac ctggggtatt
     2461 taaactcaga tatgagcgaa cagaagactt tgaaatttct ccaattttta aataaacagc
     2521 ttttgttatc tcctcaatag aagcttctct tccaagcctt aaaaaacatt caattatcga
     2581 gtcacgaaca acaccaggag ccaaagacat gtttataaat ccagtatttt tatataaaat
     2641 aataatacta actagacgtc aggacgtcaa tttagtttaa aagagacgtc aaaaaagtat
     2701 acattaactt tttttttacc ataacatccc ttacccgcac cgcgccggag gccctcaggg
     2761 cgtttctgat ggctgacggg caaaaggtgg tggcaagtac accgaaaacc accagaaacg
     2821 cacctgattt attttacgca tatgcatcac ttattcacat cactgcatag ccggtgattt
     2881 cggttaaaaa agactctctg tgtgctgaaa tcgcagaaaa tggccatgat ccccgtcacg
     2941 ctcaggccac ctgagacggg cgtttatcag tctccatact ccctgtcacc atgagataat
     3001 gtgcgttgcc gttccggtac cagatctgct gtcttctctg gttccttttc cgttctttgc
     3061 ccgtatcgct gttgctcaat ctcattcagc ctgtcgataa ccggcgcagc tgctcgctca
     3121 aactctgcac ctgcctgctc aagcccgaca cgcgctcgct cagcgccgcg ttctcccgtt
     3181 gcataagacc agacatcttc cgccattccc cgaaggcgct ctcccactcg tccagccttc
     3241 tcgagtagtc ctgctgtagc tgctctaacg cgctcagcaa ttgtttttcc agttccgtca
     3301 ttctgctcta ctcccgtacc agcttcaccg cgttccgcgt cttgttgtcc accgtccggc
     3361 ccgtttcggc tttcatcccc tccggcagca ccagaaaccg tccgttgcgg ttctccagat
     3421 aggtcacgcc ccacgtctgc atgtgcagtt tcgacagcgt atctctctgc tgcctgattt
     3481 ccgccaggtt cgccgctatc cggttcccct ggtaccacag caccccgctc aggctggcga
     3541 acagcagccc tgacaccatg gtgatggtca gccacgtccg cccgaccatt cgcagcacgc
     3601 tcagccggtt cgactgcatc gccgcgttca tcccctgttc gtgggcgctg atggcgtcgt
     3661 tgattctctt cgcgcttgcg ttcagttccg atttgacaac gttctcgtgc ttgctgaatg
     3721 cggctttcag cctctcttcg gtaagctgct gctgtgcttt cgatttctcc tcgaagtccc
     3781 tcgaggccgt taaaagactg ctcataaatg gctcccctca gtcggatatt tcgccccccg
     3841 tccgggtcgg caatgctgat actgtttttt gtggttctga ccacctcaaa accggccgct
     3901 gtgagcgccc ctgtgacgtc atcacgcgtt ttcagctctc ctgatgaggc taacatcagc
     3961 aaaccccgtg taatcgtctc cgcggcttcc tgcttcgttt ctggcagtcc tgacggcgtg
     4021 accagcgccc gccggttctc cgggtcgttc ggatcatgca gccccagcct gccgttgacc
     4081 actgtctgcc acgcatcgat gcgcggccgg tcggcacgat cgtaatacgg ctggaggcgt
     4141 ttccccgtca gtaattcggt attggggatc agaaaattca gctcaagacg acccttgtct
     4201 gcgtgttcca cccacagaat gctgtactga tctttatcga gtccgggcat cagaacccgc
     4261 tcaaagctgg ccatcagttt ttcacgctga cccggcggca ggtccgcctc cgcgaatgac
     4321 agcaccccgg aggtatattt tttcacatac ggcgacgcat caatcagctc ccggacttct
     4381 tccggcttcc cctgcaggac gctggcacct tcacgctgcc ggtctttacc cagaagatag
     4441 tccaccggac cggcgccccc gccgcgaccc cggggatgga atttaacaat catcgtccgg
     4501 ccctttttcc tgtacggcat gccgcaaccg ttccagaccg gcatcgatcg ccatcagcgc
     4561 tgccacgatc tgcacccggt catgaccggc accaccggcg ttcactttcc gggcgatctg
     4621 gttcaggtta ttgcccatgc cggcaagctg acgcagcagc gccggcgaaa tcgaggggag
     4681 cctgccggcg cgtgtcggtt tctcatcaag gcaggtctgg cgcatccacg ccgccagttg
     4741 ttttccgtcg caccgctcaa gcaaccgccg gtgttcgtct tccgtcaccc acatcgtgag
     4801 catcttgttg cgtttatctg ccagaatcgc ctcctgataa ccccgtgacg gacgggaaag
     4861 cctttcccgg tcggcgcggg gttggacaag ccgcaggcgc gtcagtggtt tttagcgggt
     4921 ttcggggcgc agccctga
//
