LOCUS       NZ_CP134008             3259 bp    DNA     circular CON 21-OCT-2023
DEFINITION  Klebsiella pneumoniae strain AMR200031 plasmid unnamed10, complete
            sequence.
ACCESSION   NZ_CP134008
VERSION     NZ_CP134008.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37176067
            Assembly: GCF_032829175.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Lai,Y.-C.
  TITLE     Diversification of ST11 KL64 Klebsiella pneumoniae in Taiwan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3259)
  AUTHORS   Tang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) Microbiology and Immunology, China Medical
            University, No. 91, Xueshi Road, Taichung 40402, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/21/2023 13:11:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,998
            CDSs (total)                      :: 5,874
            Genes (coding)                    :: 5,749
            CDSs (with protein)               :: 5,749
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 49 of 125
            Pseudo Genes (incomplete)         :: 73 of 125
            Pseudo Genes (internal stop)      :: 14 of 125
            Pseudo Genes (multiple problems)  :: 11 of 125
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP134008.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="AMR200031"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed10"
                     /geo_loc_name="Taiwan: Kaohsiung"
                     /lat_lon="24.24 N 120.54 E"
                     /collection_date="2019-06-04"
                     /collected_by="Kaohsiung Medical University Chung-Ho
                     Memorial Hospital"
     gene            480..1154
                     /locus_tag="REM89_RS29960"
                     /old_locus_tag="REM89_29960"
     CDS             480..1154
                     /locus_tag="REM89_RS29960"
                     /old_locus_tag="REM89_29960"
                     /inference="COORDINATES: protein motif:HMM:NF025107.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_065806389.1"
                     /translation="MIPNWAPMFSWWVHNGLIRKFNWRNDEIGKPSAKVAALMIYIAIT
                     MKCAEEVGIQEVKITYTRLSEMTGISRQLIREGTAILVKLDMIDVTRIGRNNTYKLISN
                     KERSGWCKIPVRAITSEGGHAITPFEDLKLRRKVELHALKIFIYLASVRDNYTEYSLAS
                     YQTISSAVGITEKDIQRALALLSLVGLITRISSEKADDTKKNQPNRYYLAGYKSFVKRS
                     DW"
                     /name="helix-turn-helix domain-containing protein"
     gene            1916..2107
                     /locus_tag="REM89_RS29965"
                     /old_locus_tag="REM89_29965"
     CDS             1916..2107
                     /locus_tag="REM89_RS29965"
                     /old_locus_tag="REM89_29965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_023302508.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2107..2529
                     /locus_tag="REM89_RS29970"
                     /old_locus_tag="REM89_29970"
     CDS             2107..2529
                     /locus_tag="REM89_RS29970"
                     /old_locus_tag="REM89_29970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_025714834.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKAIKVID
                     TSELIRVFGSLDGVIDTVKYDVKSDAESTSINSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKREPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(2875..3084)
                     /locus_tag="REM89_RS29975"
                     /old_locus_tag="REM89_29975"
     CDS             complement(2875..3084)
                     /locus_tag="REM89_RS29975"
                     /old_locus_tag="REM89_29975"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_158239539.1"
                     /translation="MNALKATTNHPDNGERMRNLLRGREALPAQPENRFSARSGIGFVD
                     GRQSRYGKSPDDSPHDSPAIRIVN"
                     /name="hypothetical protein"
     Typing          complement(1419..1868)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="80.0"
                     /coverage="92.0"
                     /evalue="8.57e-86"
ORIGIN
        1 gtaaatagct cgactctgtg gctcttactc gacgataaaa aaatagtatt ataaaacaac
       61 accttacaaa aaaacatata tttatcaatg gtttatgttt gttttttgtg aactaatagt
      121 ttatcaattt gggcgttttt tgactgataa atggtctttt atcgcgctaa atttcatctt
      181 atgaactaat ttttagatgc taaaaactaa tgattctaaa agtaagtgat tgattaaatt
      241 ggttttattt tttattttca aaaaaaatta aaaccattgt ctctagatgt aaatcctttg
      301 gcgagataaa atttgaaaat aactcctcgt agaaggacct tttatggaaa atttaaccgt
      361 tatcagttca tttattacct caatagcccc tgtcatcagt gggttcgttc cgttcctgtt
      421 gtcattagct gttttggcag gcttcgttgc tatttgcatt attggctgga ggaagtgaga
      481 tgatccctaa ttgggctccg atgttctctt ggtgggttca taacggactt attcggaaat
      541 ttaattggcg taacgatgag atcggcaaac catcagcaaa ggttgccgct cttatgattt
      601 acatagcaat taccatgaaa tgcgctgaag aagttggcat tcaggaagtg aaaataacct
      661 atacccggct atcagaaatg acagggatta gtcggcagct gataagagaa ggtacagcta
      721 ttttagttaa gctggatatg attgatgtta cgcggattgg tcgaaacaac acctataaac
      781 tgataagcaa taaagagcgt agtggatggt gcaagatacc tgtaagagca ataactagtg
      841 aaggtggcca tgctattaca ccatttgaag atttaaagct aaggcgaaaa gtagagctgc
      901 atgcgctaaa aatattcatt tatcttgctt ctgttcgtga taactacaca gagtattcgt
      961 tagcttctta ccagactata agtagtgctg ttggcattac agaaaaagat atccagagag
     1021 cattagcact cctctcattg gtaggtctga tcactaggat ttctagtgaa aaagctgatg
     1081 acactaaaaa gaatcagcct aatcgctact atcttgctgg atataaaagc tttgttaaaa
     1141 gatcggactg gtgaatacga ggtgtgcata ggcagggatg ggtttaacgc taagctagga
     1201 atgcattctt gacgttactc tttggctaag agtatcgtta cgcattaacg aaaaaaccgc
     1261 cctgcaaggc ggtttttcga aggttaagtc agttggggaa ctgcttaacc gtggtaacag
     1321 ggtgaacaag accgctgccg ccaaatttct ctttaagttt agccgttgtt aggctcaaac
     1381 atcaagacta aagaatctat aggcatcttg ttaccccgtt accagtggct accgccaatg
     1441 gcgatttgtc gtgtctgctc cgggttggac tcaagtgata gttaccggaa agggcgtggt
     1501 agtcgggctg aacggggggt tcttgcatac agcccagctt ggagcgaact gtctaaacgg
     1561 agcgggacgt ggtgatttag gtaaggcttc caccacaaca cgtaaaccgc agggcgggaa
     1621 caggaaagcg caagagggag ccaccggggg gaaacgcccg gtatctttaa gtcctgtcgg
     1681 gtttcgccac cactgatttg agcgtcagat ttcgtgatgc ttgtcagggg ggcggagcct
     1741 atggaaaaac ggccgctgtg ctgccttctc cctctccgag ctgtctgctc tatgtcgtta
     1801 tgtgcgcgtt gcgaccgctc gccgcagccg aacggcagga gcgtagcgac tgagtgagcg
     1861 aggaagcgga aaagagatct gtctgacatt gagcactgac gctctgaggc cctttatgac
     1921 gaaacaacag aaaacagcgc tgaacatggc taaattcatc cagaatcagt ctctgctcct
     1981 gctggagaag cttaacgagc ttgatctcga tgttgaggcc gacctttgtg agaaactcca
     2041 cgatgatgcg gagcatcttt tccgtacact gtcctcgaga ctggatgaat tacaggatgg
     2101 taattgatgg ccagagtgtc tattagtgaa gcggcacggc ttgttaaggt tagccgtccc
     2161 acaatttaca agatgattaa cagtgggaag ttaagttaca cctctgttgt aaagcacggg
     2221 aaggctatta aagttattga tacttcagag cttattagag tgtttggttc tcttgatggt
     2281 gtcatcgata ctgtaaaata tgatgtaaaa agtgacgcag aatctacaag tattaacagc
     2341 gttggtttac atgatttaca acatagaatc gctctacttg aagctgaaaa cgatggtctt
     2401 aaaggggctg ttaaggctcg agatgagcat attgattccc ttcgtcaggc tatgcagctt
     2461 ctggagcaca agcgcgagcc ttcttcacca ccccattccc cgtggtggaa attctggaaa
     2521 aaatcctgat cctgccatgc ttgagttttc ctctccggcc atcccaggga gttcctgaac
     2581 cttcccaaaa atcgccagat ctctgaacgc tcctcacgac taaaaatacc cacacactac
     2641 gcaaaattaa aattttgcag attccttatt taaaacaatt ggttaatgaa aaattaatgt
     2701 gcagtgatgt gttacaaagt gcagatccaa gtcgtggacg actctatggc gaatcgttta
     2761 cgaattgccc acgattcata tgtgaatcac gctttattat catgttgtta tgatgaatcg
     2821 actcttgatt caattcatca atttgggcgt tttttgttca tggtgactct tgattcaatt
     2881 cacgattcga atcgccggtg aatcgtgtgg tgagtcgtcc ggcgacttgc cataacgtga
     2941 ttgccggccg tcgacgaacc cgattcctga acgtgctgaa aaccggtttt ccggctgtgc
     3001 cggcaacgcc tcacggccac gcaataaatt gcgcatccgt tcaccgttat cggggtggtt
     3061 cgtggtggct ttcagggcgt tcatcggatt ttagccgcta aaatcccggc caggtatgtc
     3121 cgtgcatagc catgcagtcg cgccagaatc atcaggggcg ttttttctcg atatgggcac
     3181 agaaaggtgt gtgtttcagg ccggaggcct cagaaacgcc ctgagggcct ctgaggcggt
     3241 gcgggcaacg gatgttatg
//
