LOCUS       NZ_CP134006             4167 bp    DNA     circular CON 21-OCT-2023
DEFINITION  Klebsiella pneumoniae strain AMR200031 plasmid unnamed8, complete
            sequence.
ACCESSION   NZ_CP134006
VERSION     NZ_CP134006.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37176067
            Assembly: GCF_032829175.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4167)
  AUTHORS   Lai,Y.-C.
  TITLE     Diversification of ST11 KL64 Klebsiella pneumoniae in Taiwan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4167)
  AUTHORS   Tang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) Microbiology and Immunology, China Medical
            University, No. 91, Xueshi Road, Taichung 40402, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/21/2023 13:11:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,998
            CDSs (total)                      :: 5,874
            Genes (coding)                    :: 5,749
            CDSs (with protein)               :: 5,749
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 49 of 125
            Pseudo Genes (incomplete)         :: 73 of 125
            Pseudo Genes (internal stop)      :: 14 of 125
            Pseudo Genes (multiple problems)  :: 11 of 125
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP134006.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4167
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="AMR200031"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed8"
                     /geo_loc_name="Taiwan: Kaohsiung"
                     /lat_lon="24.24 N 120.54 E"
                     /collection_date="2019-06-04"
                     /collected_by="Kaohsiung Medical University Chung-Ho
                     Memorial Hospital"
     gene            complement(join(4094..4167,1..559))
                     /locus_tag="REM89_RS29905"
                     /old_locus_tag="REM89_29905"
     CDS             complement(join(4094..4167,1..559))
                     /locus_tag="REM89_RS29905"
                     /old_locus_tag="REM89_29905"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3226 domain-containing protein"
                     /protein_id="WP_012579086.1"
                     /translation="MKDINNSKKIIFVEGDDDFHFLCNLLEIQGITDVFVEKINGKSKL
                     KQALRAFKNIDSFDEKESIFIILDADTSFSDTERSIIATLRELQISSPSSHNEIAMNGN
                     TKISFFIMPGKDKQGAIEDLIIEHASTRALYEYIIEFFEKIKNNKDDISASDKEYTYPT
                     NEKKAKVQVYLSCNRESDSRIGISMKNGIINTADNCFDEIKDFISKI"
                     /name="DUF3226 domain-containing protein"
     gene            complement(559..1674)
                     /locus_tag="REM89_RS29910"
                     /old_locus_tag="REM89_29910"
     CDS             complement(559..1674)
                     /locus_tag="REM89_RS29910"
                     /old_locus_tag="REM89_29910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_012579087.1"
                     /translation="MIKEISLKNFKCFNELYLKQLKTLNIIAGKNNYGKTSILDAIFCF
                     YDVKNPAVLLNIQAFRKEMAEINKNKPFWVSYFHDMDTSQKMSIVIRDERSEVTQTYET
                     ETNQRLESSLSLNVDTVLSNQNIPRQTIGSQATVNSLKINVTETPNAKNRVSNKLFSMQ
                     LKEDGVEIKSEIKNERDGINYKFKTATIITTSRKINKEATITNVSSLLTQKRKKDILEN
                     LKKIDDRIVDIAISAIGNNKEIYLDIGFSELNEISMLGEGISRALSFISSVLVQENSII
                     LIDEIENGIHYSVIKDIIKSLISSAKQNNNQIFATTHSQDVIRAINEIDSKNEDIAYIR
                     LGREKNSLKPTAVQFNMDDFSYSVENDWEVR"
                     /name="ATP-binding protein"
     gene            complement(2019..2132)
                     /locus_tag="REM89_RS29915"
                     /old_locus_tag="REM89_29915"
                     /pseudo=""
     CDS             complement(2019..2132)
                     /locus_tag="REM89_RS29915"
                     /old_locus_tag="REM89_29915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            2548..2739
                     /locus_tag="REM89_RS29920"
                     /old_locus_tag="REM89_29920"
     CDS             2548..2739
                     /locus_tag="REM89_RS29920"
                     /old_locus_tag="REM89_29920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2739..3140
                     /locus_tag="REM89_RS29925"
                     /old_locus_tag="REM89_29925"
     CDS             2739..3140
                     /locus_tag="REM89_RS29925"
                     /old_locus_tag="REM89_29925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_012579088.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(3486..3728)
                     /locus_tag="REM89_RS29930"
                     /old_locus_tag="REM89_29930"
     CDS             complement(3486..3728)
                     /locus_tag="REM89_RS29930"
                     /old_locus_tag="REM89_29930"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_098963735.1"
                     /translation="MHSAEIHTIQPGEVLFPAFFSSENGERGRNLLRGREALPAQPENR
                     FSAPSGIGFVDGRQSRYGKSPDDSPHDSPAIRIVN"
                     /name="hypothetical protein"
     Typing          complement(2020..2427)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.399"
                     /coverage="99.0"
                     /evalue="5.92e-171"
ORIGIN
        1 atattcctat tcttgagtcg gattctctat tgcagctaag atagacttgt accttagctt
       61 ttttttcgtt tgttggataa gtgtactctt tgtcactcgc tgagatgtca tctttattat
      121 ttttaatttt ttcgaaaaat tctattatgt attcatataa cgctcgagtg cttgcgtgtt
      181 caattatcag atcttcaatg gcaccttgct tgtcttttcc tggcattata aagaaagaaa
      241 tttttgtatt gccattcata gctatctcgt tatgcgagct gggggaggag atttgtagtt
      301 ctcgcaatgt ggcaattatt gatctttctg tgtcagaaaa tgatgtgtca gcatctaata
      361 taataaaaat actttctttt tcatcgaaag aatcaatgtt tttgaatgct ctaagtgctt
      421 gttttaattt tgattttcca tttatttttt cgacaaaaac atcggtaatg ccttgtattt
      481 caagtaggtt gcatagaaaa tgaaaatcat catcaccctc aacgaaaatg atctttttgc
      541 tattatttat gtctttcatt atcttacctc ccagtcgttc tcaactgagt aggagaaatc
      601 atccatattg aattgtacgg ctgttggttt tagggagttc ttttctcttc ctagtctaat
      661 ataagcgata tcttcatttt tgctatctat ttcgtttatt gctcgtatca catcttgact
      721 atgagttgtt gcaaatattt gattgttgtt ttgttttgca gatgaaatta atgattttat
      781 tatgtcttta attactgagt aatgtattcc gttctcgatt tcatcaataa gaataattga
      841 gttttcctgt accagcactg agctgataaa agataatgct cgactgatac cctcgccaag
      901 cattgatatt tcatttagct cactaaatcc aatgtctaaa tatatttcct tattgttacc
      961 tatcgcagat attgctatat ctacaattct gtcatctatt ttttttaagt tttctagtat
     1021 gtcttttttc cttttttgtg ttagtaggct agatacattt gtaattgttg cctctttgtt
     1081 tatctttctg gatgttgtta ttattgttgc tgtcttaaat ttataattaa tcccatccct
     1141 ttcgtttttt atttcggatt ttatttctac gccatcttct ttcaattgca tagagaaaag
     1201 cttattggaa accctgtttt tcgcattggg tgtctcggtc acatttattt ttaaagagtt
     1261 tactgtggcc tgagaaccga tagtttgacg aggtatgttt tggttagata gtacggtgtc
     1321 aacgtttaga gacaaacttg attctaatct ctgatttgtt tctgtttcat atgtttgtgt
     1381 tacttcagag cgttcatctc gaatgactat ggacatcttt tgtgatgtgt ccatatcatg
     1441 aaagtaacta acccaaaatg gcttgttttt gtttatttct gccatttctt ttctgaatgc
     1501 ttgaatgttt aacaaaacag ccgggttttt tacatcataa aaacaaaaaa tagcatccag
     1561 tatagatgtt ttcccgtagt tgtttttccc agctatgata tttaaagtct ttaattgctt
     1621 tagatatagc tcattgaagc atttgaagtt ctttaaactt atttccttta tcatgatagc
     1681 ctctgttcgt gtttttgtta tatgccatac gatttgttag taccttaata aaatgtacag
     1741 ctttcattgt gattagctag actttatgtc aaagattaac tttgattgct tgtgttttaa
     1801 tcgatttttt ttactttctt cttgagatta tctggcagca gccgtaagct tccggctgtg
     1861 aacgaaaaaa accgccttgg cgggcggttt tttcgaaagg ttcagtagag ttggcgctct
     1921 atgaaccgag gtaactggct taaagggagc gcagtcacca aaactgttcg tttagtttag
     1981 ctttaactgg cgtataactt caagactaac tccctaaatc agttaccagt ggctgctgcc
     2041 agtggcgctt tgtcgtgtct ttccgggttg gactcaagac gatagttacc ggattaggcg
     2101 cagcagtcgg actgaacggg gggttcgtgc atacagtcca gcttggagcg aactgcctac
     2161 ccggaactga gtgtcaggcg tggaatgaga caaacgcggc cataacagcg gaatgacacc
     2221 ggtaaaccga aaggcaggaa caggagagcg cacgagggag ccatcagggg gaaacgcctg
     2281 gtatctttat agtcctgtcg ggtttcgcca ccactgattt gagcgtcaga ttccgtgatg
     2341 ctcgtcaggg gggcggagcc tatggaaaaa cggccgctgt gcggcctttc cttctttcct
     2401 ccatgcgatt actcggtttt ctcgagcgtt tctacgcatg ctcctgccgc tcgccgcagc
     2461 cgaacggcag gagcgtagcg actgagtgag cgaggaagcg gaaaagagga gatccctaca
     2521 ctgaccactg acgctctgag gcctcttatg acgaaacaac agaaaacagc gctgaacatg
     2581 gctaaattca tccagaatca gtctctgctc ctgctggaga agcttaacga gcttgatctc
     2641 gatgtcgagg ccgacctttg tgagaaactc cacgatgatg cggagcatct tttccgtaca
     2701 ctgtcctcga gactggatgc attacaggat ggtaactgat ggccagagtg tctattagtg
     2761 aggctgcccg actgacgggt aagagtagaa caacactgca caggttaatt aaagcaggtg
     2821 acctgtccac ctgtagcggt gagcgcaatg caaaaatggt agatacttca gagcttttac
     2881 gtgtctttgg cccctttgag caaccgaagg gtgaacacca ttctggacag gtctctggac
     2941 agcgtgatac agcctctgct gaccagagtg aacaggtgat ccgtcatctg aaacaggaga
     3001 ttgaacacct gaacacctta cttttgtcca aggattcgca cattgacagc ctgaaacagg
     3061 ccatgctcct tcttgaaaat caccaggcaa aattaactga atccccggcg ccatggtgga
     3121 aattctggaa aaaatcctga ccccgtcatg cttcagtttt cctctccggc catcccaggg
     3181 agttcctgaa ccttcccaaa aatcgtcaga tctctgaacg ctcctcacca ctaaaaatac
     3241 ccacacccca cgcaaaatta aaattttgca gattccttat ttacaacaat gggttaatga
     3301 aaaataaatg tgcggtgatg tgttacaaag tgcagatcca agtcgtggac gactctatgg
     3361 cgaatcgctg acgaattgcc cacgattcat atgtgaatca cgctttatta tcatattgtt
     3421 atgatgaatc gactcttgat tcaattcatc actttgggtg ttttttgctc atggtgactc
     3481 ttgattcaat tcacgattcg aatcgccggt gaatcgtgtg gtgagtcgtc cggcgacttg
     3541 ccataacgtg attgccggcc gtcgacgaac ccgattcctg aaggtgctga aaaccggttt
     3601 tccggctgcg ccggcaacgc ttcacggcca cgcaataaat tgcgtccccg ttcaccgttt
     3661 tcggaggaga agaacgccgg aaacaggact tcccctggct gaatggtgtg aatttccgcg
     3721 ctatgcactt gcgcgcatac tcatgcatgc cgtaaaaaca gagcctgcgc gtttctggcg
     3781 ggttttcggg tggtttgttg cctgttttac cggtttcccg tcagaaacgt cctgagtccg
     3841 ttttagcggt gcgtacaatt atgattttat gttaaattgt tttaagtgaa tagagagaat
     3901 ataacttaag ggagcatctt gtgctgggtc ttttacatta gatctgcaca atcattgttt
     3961 tggcatctgg tgatgtcttt aggacgtttg agattgctga taactaaagt gtatgatgct
     4021 ttctatacgt tatccggcgt ttaataacac cctggtgata agccagcccg tgaagggctg
     4081 gcttgatttg gatttatatt tttgaaataa aatcttttat ttcatcaaag caattgtctg
     4141 ctgtgtttat tattccattt ttcatgg
//
