LOCUS       NZ_CP134005            12764 bp    DNA     circular CON 21-OCT-2023
DEFINITION  Klebsiella pneumoniae strain AMR200031 plasmid unnamed7, complete
            sequence.
ACCESSION   NZ_CP134005
VERSION     NZ_CP134005.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37176067
            Assembly: GCF_032829175.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 12764)
  AUTHORS   Lai,Y.-C.
  TITLE     Diversification of ST11 KL64 Klebsiella pneumoniae in Taiwan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12764)
  AUTHORS   Tang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) Microbiology and Immunology, China Medical
            University, No. 91, Xueshi Road, Taichung 40402, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/21/2023 13:11:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,998
            CDSs (total)                      :: 5,874
            Genes (coding)                    :: 5,749
            CDSs (with protein)               :: 5,749
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 49 of 125
            Pseudo Genes (incomplete)         :: 73 of 125
            Pseudo Genes (internal stop)      :: 14 of 125
            Pseudo Genes (multiple problems)  :: 11 of 125
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP134005.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12764
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="AMR200031"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed7"
                     /geo_loc_name="Taiwan: Kaohsiung"
                     /lat_lon="24.24 N 120.54 E"
                     /collection_date="2019-06-04"
                     /collected_by="Kaohsiung Medical University Chung-Ho
                     Memorial Hospital"
     gene            complement(259..378)
                     /locus_tag="REM89_RS29830"
                     /old_locus_tag="REM89_29830"
     CDS             complement(259..378)
                     /locus_tag="REM89_RS29830"
                     /old_locus_tag="REM89_29830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368419.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_194315302.1"
                     /translation="MGVITGFLLAACQANYIRDVQGGTVAPSSSSELTGIAVQ"
                     /name="colicin release lysis protein"
     gene            complement(379..1076)
                     /locus_tag="REM89_RS29835"
                     /old_locus_tag="REM89_29835"
     CDS             complement(join(379..827,827..1076))
                     /locus_tag="REM89_RS29835"
                     /old_locus_tag="REM89_29835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558267.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="WP_223174630.1"
                     /translation="MASVSVCCPSCSATEGVMRNGKSTAGHQRYLCSHCRKTWQLTFTY
                     AASQPGTHQKIIDMAMNGVGCRATARLMGVGLNTILRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEQWGYVGAKSRQRWLFYAYDRIRRAVVAHVFGERTMATLERLLSLLSVFDVVVW
                     MTDGWPMYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1 family transposase"
     gene            complement(1270..1527)
                     /locus_tag="REM89_RS29840"
                     /old_locus_tag="REM89_29840"
     CDS             complement(1270..1527)
                     /locus_tag="REM89_RS29840"
                     /old_locus_tag="REM89_29840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(1537..3222)
                     /locus_tag="REM89_RS29845"
                     /old_locus_tag="REM89_29845"
     CDS             complement(1537..3222)
                     /locus_tag="REM89_RS29845"
                     /old_locus_tag="REM89_29845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            complement(3327..4814)
                     /gene="ltrA"
                     /locus_tag="REM89_RS29850"
                     /old_locus_tag="REM89_29850"
     CDS             complement(3327..4814)
                     /gene="ltrA"
                     /locus_tag="REM89_RS29850"
                     /old_locus_tag="REM89_29850"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     gene            5459..5704
                     /locus_tag="REM89_RS29855"
                     /old_locus_tag="REM89_29855"
     CDS             5459..5704
                     /locus_tag="REM89_RS29855"
                     /old_locus_tag="REM89_29855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001792445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004146442.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNRTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEAAETTFTWQPLRRR"
                     /name="hypothetical protein"
     gene            5978..6349
                     /gene="tolA"
                     /locus_tag="REM89_RS29860"
                     /old_locus_tag="REM89_29860"
     CDS             5978..6349
                     /gene="tolA"
                     /locus_tag="REM89_RS29860"
                     /old_locus_tag="REM89_29860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            6346..6564
                     /locus_tag="REM89_RS29865"
                     /old_locus_tag="REM89_29865"
     CDS             6346..6564
                     /locus_tag="REM89_RS29865"
                     /old_locus_tag="REM89_29865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002193751.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_002193751.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHFFFGKR"
                     /name="TonB family protein"
     gene            6906..7184
                     /locus_tag="REM89_RS29870"
                     /old_locus_tag="REM89_29870"
     CDS             6906..7184
                     /locus_tag="REM89_RS29870"
                     /old_locus_tag="REM89_29870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            7172..7486
                     /locus_tag="REM89_RS29875"
                     /old_locus_tag="REM89_29875"
     CDS             7172..7486
                     /locus_tag="REM89_RS29875"
                     /old_locus_tag="REM89_29875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006120406.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_001136729.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKAGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYQELVIGFGDSGYVMLYRHDREMDRIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            7650..8078
                     /locus_tag="REM89_RS29880"
                     /old_locus_tag="REM89_29880"
     CDS             7650..8078
                     /locus_tag="REM89_RS29880"
                     /old_locus_tag="REM89_29880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            8104..8283
                     /locus_tag="REM89_RS29885"
                     /old_locus_tag="REM89_29885"
     CDS             8104..8283
                     /locus_tag="REM89_RS29885"
                     /old_locus_tag="REM89_29885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_065521055.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMQDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(8311..8841)
                     /locus_tag="REM89_RS29890"
                     /old_locus_tag="REM89_29890"
     CDS             complement(8311..8841)
                     /locus_tag="REM89_RS29890"
                     /old_locus_tag="REM89_29890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_065521056.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMAVMAWQTGAYVFRFE
                     DQAQHLAMMKFIGMYHERLNSDDKATYYFTGKNPAWEREKPAGMKLCDYLYRKVEQFAI
                     ENGVQVLDAGKYTPEQIGAMIDVVSERYGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRRWPFGKK"
                     /name="hypothetical protein"
     gene            complement(8848..9579)
                     /gene="mobC"
                     /locus_tag="REM89_RS29895"
                     /old_locus_tag="REM89_29895"
     CDS             complement(8848..9579)
                     /gene="mobC"
                     /locus_tag="REM89_RS29895"
                     /old_locus_tag="REM89_29895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_317085478.1"
                     /translation="MALERYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVAQRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDIIPFQP
                     SRVSAAQLPHRLAVQSLRLAMEARGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(9579..11540)
                     /locus_tag="REM89_RS29900"
                     /old_locus_tag="REM89_29900"
     CDS             complement(9579..11540)
                     /locus_tag="REM89_RS29900"
                     /old_locus_tag="REM89_29900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_065799589.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNNRTAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRWWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVDRREIPLKTFPVLAKTDPLAEY
                     QSEAGRQRPGDDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     BGC             1..8222
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          12342..12764
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.418"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(8852..9579)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.827"
                     /coverage="100.0"
                     /evalue="4.44e-141"
ORIGIN
        1 cagccattgg taactgggag ttcgcagagg atttgtttag ctaaacacga ggttgctctt
       61 gaagtgtgcg ccaaagtccg gctacactgg aaggacagat ttggttgctg tgctctgcga
      121 aagccagtta ccacggttaa gcagttcccc aactgactta accttcgatc aaaccacctc
      181 cccaggtggt tttttcgttt acagggcaaa agattacgcg cagaaaaaaa ggatctcaag
      241 aagatccttt gatcttttct actgaaccgc gatccccgtc agttcagagg aggacgatgg
      301 tgccaccgtt ccgccctgaa catcccggat ataatttgcc tgacatgcgg ccagaagaaa
      361 gccggtaatg actccaattt actgatagtg ttttatgttc agatagtgcc cgatgacttt
      421 gtcatgcagc tccaccgatt ttgagaacga cagggacttc ctgcccagcc ttgccagatg
      481 ctgtctcaga ttcaggttgt gtcgctcaat tcgctgcgtg taacgcttac tgatgacgtg
      541 cagttttccc ttcagacgtg actcatacat cggccagccg tccgtcatcc ataccaccac
      601 gtcaaaaact gacagcaggc tcagaagacg ctccagtgtg gccatagtgc gttcaccgaa
      661 aacgtgggct accaccgccc tgcgtatcct gtcatacgcg taaaacagcc agcgctgacg
      721 tgattttgca ccgacgtagc cccattgctc gtccatctcc gcacagacaa tgacatcact
      781 gcccggctgt atccgggagg ttactgactg cggcctgagt tttttaaatg gcgcaaaatg
      841 gtgttgaggc ccacgcccat tagtcgtgcg gtggcacggc aaccgacgcc gttcatagcc
      901 atatcaatga ttttctggtg tgtaccgggc tgagaagcgg cataagtgaa ggtgagctgc
      961 catgttttac ggcagtgaga gcagagataa cgttgatgcc cggcagtact tttgccatta
     1021 cgcatcacgc cttcagtagc ggaacaggag ggacagcaga cggagactga agccacggga
     1081 gcacctcaaa aacaccatta tacactaaat cagcaagttg gtagcatcac caagaaagcc
     1141 agcaacaatc aatataaaaa gaaaaatagt ctttaccttt ttcatatact ttccctgtat
     1201 ttaaagactc cggcattcat ccctgaacac cggaagtcag tatttcaaat catccttgat
     1261 aattttttgt taccagttac cgtcccgata atcaaaagct atataacaat tatccacatc
     1321 aggttttatt tgatttttaa aaaatggctg tagtaaagga acccattctt tcttcaaatc
     1381 aaactcacca ttattaatat tatcctttaa aggcattccc aagctttcaa tgacagaacc
     1441 atcgtcacca aagtctttag aatactcttc ccctttaaac tcttcagttt tcttatcaaa
     1501 ccatgtcaaa cgtaatttaa gccccatatt tacctctcaa agatattttt taatgttccg
     1561 ttttggatct ggccctttaa cctgcttacc cgttttggga tcaaatgccc cgaggtgttc
     1621 gccatcactg gcacgatacc cttcaagctc accgtgctgg gaatcccatt cataaatttt
     1681 acgtttttta tcgccatacc atcgggcccg cttaccacca ccaccttgtt taggtgtttt
     1741 aggatcacct cgctttaact ctccaagtcc ctttatgtct tctgttttag gtactggatg
     1801 ataatcatgg ccgtagtctt tagtcccctt gcgaggcttg ttcttttcct catttaactt
     1861 attttctgca tccttcgctt ttttctcctt ttgtttccgg ctctccatcg ccccggtaag
     1921 tgcagcctta tctctggata aggattcatt cattgcgttt aaagatgcct gagccgcttt
     1981 taatttctca ttctgattct gaatatcctt atcaagatag cgaacctgat cctgccaccc
     2041 ggagccatat tcatgaggcg ggttataagt cacaaaatca tctttatttg cctctgcctc
     2101 ttttaccttt ttatcggctt cctgaacagc agcccttgca ccggggagcg ccttctctgt
     2161 cgatgcaatg cggctgttta aggtcgcaat atttttatct tcggcatcca gctcagcttt
     2221 cgctttatca tagtctcttt tcagcccctc ttccggatga gcggcgtccc atgcctgctg
     2281 gcgacgcttt tcttcctcct gacgctgttt tacctgtgcc ggggtaagaa catctgtcac
     2341 cgacacataa accggcttct gtccggtctc tttcgggaaa cgaataacgg cctcacggga
     2401 gttaccaccg gctgtaaaac cagccggacg tgaatcacct ttttcagcaa caatgccttt
     2461 tggcggggct ttcgctgtcg gaacaccttt cggtacgctc acctgcagag acgggagacc
     2521 cggaatcgac acactgaata cccccggacg ttttgtcggt ttcgcatcca ccacagggac
     2581 actcattggc cgacctgcga caaccgcaat atgctgccgc tcatccttca ccacatccac
     2641 aacccgttgt ctgacacgaa ccgtcgcctg gtccagtggt aaagtacttg ccggagtctc
     2701 cgtcaccgca tcggccggta atgacgtcat aatttttgac atcattttcg gatcatcttt
     2761 tgcaatctca gaaggcagca caccgtagat cgcaatcccc cacagaccaa acttaaacgg
     2821 ccccttcagg gcagccagca catcagcaac ggcggccgac aacgcatcgc cggaaactga
     2881 taaagccagt ccttcagccc ccggagtagc cagggcaggc agaccgaagg ccatcgcagt
     2941 ggccgaagac tgtccgccac cagagctacc gctatgattg ccgctgttac caccattacc
     3001 accgggttta ctcccgccgt taccaaactc ccccggatta tagttatgaa tatctccatt
     3061 cggcgtatgc gtcgtacccc agcccggccc gctgtttgga tccgtaccac cacctgatga
     3121 agggccacct ttaccagacg taccgttcac attcccactg gcctgaccac cattatgtcc
     3181 cagacctgaa ttacccggac ctcgaccgtc tccaccactc atatcgtttt cctctttgac
     3241 atttaaaaca ataaattaaa gccaacatgt cgcggtaggg atacccgtta ccggataccc
     3301 cccgcacaga tcccggcgtg cgcgatttac gcaccgggct cctgcctcgg gtgtctggcg
     3361 gtgaaccgct ccacaggcca tggatgaaga acccgaaccc ttggtagcca tgcggctgcc
     3421 agtttgtttg ctttcgtcca ggtcgtatca tccttctggc tcctgcgcct gagcgcccgg
     3481 cgccagaggt ttgttacgtg tgtcctgaac ttctgcatgg tggggaagtt gcccggtacc
     3541 gagtgatagt tcaggtatcc ctgaaccact ctcctgagcc attttccctg ttcggggatt
     3601 gagtaatgcc agcgccttcg cagaccgtct ttgatggctt tcagagttgc cgtcatccga
     3661 tcccggcggg tctttcgtat cagcatgaac ctgccgttgc gatctttccc gctgatgtgc
     3721 gtgaacccga ggaagttgaa cgtttctggt ttgccttttc ccctgatggc acggttttcg
     3781 gcagcgaagc ggccgaactc catcagacgg gttttctccg ggtgaaccgt gagtccgaac
     3841 tccctcagtc tgcgctgcat ggctatacgg aagcgccggg catcgtatcg tttgtcgaac
     3901 ccgatgacga tgtcatcggc gtatctgacc attaccacat tgcctgtggc atagcgacgt
     3961 cgccactgat gcgcccacag atcgaagacg tagtggaggt atatgtttgc cagcagcggt
     4021 gagatgaccg caccctgtgg ggtgccttcc tccgttgctc gccattgacc ctcctccgac
     4081 gtcccggctg tgagccactt acgtatgagc ctgattaccc tccggtcgcc gatccgatgc
     4141 tctgtgaacc tgatcagcca ttcgtggctc accctgtcga agaactgact gatgtcggca
     4201 tccagtaccc agtttacgtt agtgcgtacc agccctgtgg ccagtgcgtc cagtgcatcg
     4261 tgctggcttc gcccgggtct gaacccgtat gagaacccca taaagtcgtt ttcatagact
     4321 gcgttcagga ttttcaccag cgcatactgg acgatcttgt cctccagcga ggcgatgccg
     4381 agcgggcgtt gttttccatc cgcttttggg atgtagtgac gcctgccggg ctgcgccctg
     4441 tagctgccct gatgtagcct ccggtgcaga tctgttatgt tgttcttcat gtttccggcg
     4501 tagtccatcc acctgatgcc atccactccg gcggccgctt tcctgctcag ggagaggaat
     4561 gcggcttcca gtgcttcgac tgtcagcagg tggaacaatg ctgtaaaccg ttctttcttc
     4621 cgctgcttcg cagcttcccg cacgcgtgac agcctctgtg acatgctttc ccggctctgt
     4681 gtccggcgca tgtgtggctg ttccgcgttc cccttggccc cgctccttcg ctccactgac
     4741 tccgctcctt tcgggttgtt cgcctgcttc gccgctacta tgagcgagtc cgacttctcc
     4801 tctccgtaca tcaccggcta tgactcctcg tcttcccggt gcgggccatc tccgacactg
     4861 gcagatggtc agaggggaga tctcccggtt cccgcgtaga gatcgtattg acatgccagg
     4921 gtctcagacc ccgccgggtc catgtggcac tcgcagtatc gcaccctatg atgttgcctt
     4981 ccgttaacag tacaacgtcg gcacccggta atttaatata catttcgtgg ctcaatggct
     5041 ggcctgtcaa cacccctgtc aacgcttcgc cccatacctc gcggtatgca acgcatgact
     5101 cggggacctt gtggattgct ggtccttcaa tggtcgggga ctttcacccc ttgatctcta
     5161 accggtctcc cggcgcacac tgtacatata cacagtataa ttattacctt actcctcccg
     5221 ttttcaggtg tcaatatatg agattagttc tcatcttcag gcacaaaaaa gcgcccggag
     5281 ccacctgtaa gcagatcgcg tcttttcccg aaaatttctc acggcaacaa attttattat
     5341 ttgagatgta catcacactt acatttcagg tggtatagac acatctgaaa gctaactggt
     5401 atgaggggag gacccgcggt taaactgttc aggaaacctg agacgcccgg aggccaccat
     5461 gacacacctg aaactgactc ccgctgaaga aaagcgccta gcggacgcct ttgtcgctca
     5521 ggcactggcc atgggcctga aagggaacag aacgctgcca aaggcggtaa aacagcaggt
     5581 gcgggaaagg gtgcttcgcg ccattcagga cgaacgtgaa gccaggcggg cgcaggaaat
     5641 gcgtcgggag aaatacgagg cggcggaaac caccttcacc tggcagccgc tgcgccgccg
     5701 gtgaggggta tttaccataa aacccgttat cagcaccgcc cgagaagccc tcagagcgtt
     5761 tccggcggct acgggataaa acaggcaaca aatcacccgg aaaaccgccg gaaacgcgcc
     5821 cggtaaattg taacccgctg catgcctatg cacgcaactg catggcgggc cgtttggtgt
     5881 aaaaacgata cgataagccg tgtttatgtc aatgaacgtg gccgtaaaga gtggcaaata
     5941 tacatgtaat attttttact gacaggtcat caacccgatg cgtttaatac cctttatcct
     6001 gctggcattg tgcagcccct tactcctgac ggcctgtaag cattccacca cccagccgcc
     6061 gaaaatttat actctggata agtcatctaa aatcagtagc tacgctgcgg ccatcaagac
     6121 cgctgttgaa atgcagatgt ttgaaccaga ggcctatgcg ggaaaaatgt gcaccgtgcg
     6181 gatatccata caacgtgatg gcactctgaa cagcgccatt gctgaaggag gagatccgaa
     6241 actctgtaaa gcagcaatat cagccgttac ccacgccaaa attccgccgg caccggatga
     6301 aaatacctgg caaatattca aaaacgcccc tctggatttc agaccatgaa agcttttact
     6361 ctgacagtgc tggcggccat gttcctcact ccggtacatg cgataccact aaaacccgta
     6421 accacactgc agccaaccta cccggtacag gccgcagcaa aacgcatcga aggtcaggtg
     6481 accgccacgt ttgatattct ggacgatgga cgagtggaca acgtgtcgct taccggctgt
     6541 cacttttttt tcgggaaacg ctgacggcaa tgaggcactg gctatatccc gcagggcacc
     6601 cggcaatgcg acaggttata aattttgaat tcagtctgaa aaatgacggc ttacaggatc
     6661 cgcgaccagc caccaccaaa aagttcaatg atgatggttg ccgccagtaa caggtccttt
     6721 aacccgcagg ctgcaatagt cctggatgat gagtgagcct gtagcagccc tgattagtgg
     6781 aagcgaagct gaaaaatggg gaagcgaagc tgaaaaatgg gtagcaaaag ccgtatttct
     6841 ggcaaatagc gccgccacgg attaaagtag tcatatgtat tacacatgac tacaaagggg
     6901 cttttatgtc atcagcaaca tccatcaggc tcgatgaaga acttaaggac cggctgaaaa
     6961 cgctggccga tgaccggcac cgctcagcgc acgcgctaat gctggaggca atcaccgaat
     7021 acatcgaccg ggaagagaag cgcagccagt acctgcggga cggacaagcc gcatggcagc
     7081 actatcagga aacgggtctg caccttactg cggaagaagc agaagcctgg ataagtacct
     7141 ggggaactga gaacgagcag gatgctccgc catgccacag gtaacgattt ccgcgctggc
     7201 acagcgagat ttacaacgcc tccaggactt tctcaaaacc aaaaatcggc tggcagccag
     7261 aaaagccggt gaggtgatcg tccgggctat ccagcaactg aaaacattgc cagacattgg
     7321 ccgcccggtg ccatttctgc cgctggaata tcaggaactg gtgatcgggt ttggcgacag
     7381 tggctatgtg atgctctacc gccacgaccg ggaaatggac cggattgtga tcgtcaccgt
     7441 caggcatcag aaagaatccg ggtatccggg ggcagacagc ctctaataac cgatgacggg
     7501 ggcgcaattc attgcgtggc cgtcgccggt ttgacaagca cagcgcgtca gaaaaccaca
     7561 tcctgaacgg aaaaacgggt aaaattcagc gatacattta ccgtgagaaa ttacacatct
     7621 ttcacacccg ataaaatcag gggtatacag tggcgaaaac attgctcaac ctttcacagg
     7681 cagcacaagc cgcgggcatt accagaagaa cgctatacaa tcatgtaaaa cagggaaagg
     7741 ttaccgtgtc acgtgacggg aaaaacaacc ctgtcgtgga tgtgtcagaa ctgatccgtg
     7801 tatacggaaa tgtgaatatc cctgagaaac aaattcccgg catttcacac cgtgaaaata
     7861 cacaaaaaaa tttcccacag gaacagcttc tggcaatgca aaaggaactg gccgatctcc
     7921 ggcaggctgt caccctgatg ctggaagata aaacatcccg agaagaagaa cgccgccagc
     7981 acgatgatga gcgccggaaa ctccaggccg aagtggacag attgacaaca gaactgacac
     8041 agaagaaaaa acgcttctgg tccggctggt ttagctgaga acttaccgaa gggaatggag
     8101 aacatgacga agcaggaaaa aaccgccctc aatatggcta aattcattaa ggaccagtcg
     8161 ctgctgttgc tcgagaaact gaacgagctg gatatggata ccccggccga catgtgcgag
     8221 cggctgcacg aggatgcgga aacactctgg caggtgatgc aggataagct cggggaagaa
     8281 taacagcacc ggcatggccg gtgctggcgt ttattttttg ccgaacggcc acctccggcg
     8341 ggcgggttta tcctccggca gttctggcac cggaacgtta aaatagcggc tgttatattc
     8401 ccgcatcagc tccgctttca gcttctcgtt caccgcgctt tccccgtacc gctcactcac
     8461 gacgtcaatc atcgccccga tctgctccgg ggtgtatttc ccggcgtcca gtacctgcac
     8521 gccgttctcg atggcgaact gctcaacctt gcgataaaga taatcacaca gcttcattcc
     8581 ggccggtttc tcacgttccc acgccggatt tttgccggtg aagtaatacg tggctttgtc
     8641 atcgctgttc agccgttcgt gatacatgcc gataaacttc atcattgcca gatgctgggc
     8701 ctgatcttca aacctgaata catatgcacc ggtctgccag gccatgaccg ccatgtcttc
     8761 cagatgctcg ctggttggca taatgcagcg ctcgccgtct ttgtcttcca cgtcctcaat
     8821 aaataccatg tacagcgcca taatccctca ctccgttgtc ataaatttaa agcatgcgaa
     8881 cagcctctcc cggtccagct ccgccgtttt gcgggcctcg ccggtctgcg ggttcacgat
     8941 ttcatccacc gccagaatcg cccgctgtac ccggacaagc gtcggccggt cagggcagat
     9001 gtaccagatt tcatcgatgc cgttcgcccg gcggttgaac agatacccgg acaccacttc
     9061 ctgataccgc ctgcggctct tgacggtgcg ctctacctca aatgccaccg ttctgccgtc
     9121 cagctccgcc agcgcgtccg gcactttcat ccctttcagg gccatacggt gcaggtaccg
     9181 ccagccggtg gccccccggg cctccatggc gaggcgcagg gactgcacgg ccagccggtg
     9241 cggcagctgc gccgccgaca cccgcgacgg ctgaaacgga ataatgtccg tcagcggctc
     9301 gtcctcgtcg aacgagaacg ccaccccgtg cggcgtcagc ccccacacgc tgacctgacg
     9361 gccgtcaacc gggtatttcg cccggaccag cagccctttc gccaccaggc tgtccaggaa
     9421 gcggtaaacc ggctgtaccg ccgcaatccc cagccgttgc gcaaccagct ccgccgtgga
     9481 acacagttct tcccgcagcc agcgcagggt cagccgtgtt ttctccgcgt tccgtgcctg
     9541 ccgcttcgcg tggctgacgt tgtatcgctc cagtgccatc agtacagccc cgcaaaatca
     9601 tcatcacccg gccgctgccg cccggcctcc gactggtact ccgccagcgg gtccgttttc
     9661 gccagcacag ggaaggtttt cagcggaatt tcccggcgat caacacgcag cgggctgctg
     9721 aacgccagcc gcgccacgcc cagcccggtc atcaccgcga acccgtcagg cagatgttgc
     9781 agggtgttca cgtcaaacag cggccgcgcc tccttctgga tgaagctgtc cccgctgata
     9841 tgctccacgt tccccgcctc ccggctggcc cggcggcgct ccacgtccac ccggatctcc
     9901 ccggtctgtc cggccgccca ctgagcggac tcctggctgg ccgcccggta gaaccagcgg
     9961 atcggcgtgt tatccagtac cgtggttttc acaaaatcag cgggcagatc ctgcccgcac
    10021 tggccgaagt cgcccagcga ctggtgcgcg agcagcaggt tgcagtcccg gctgcgcagc
    10081 gtccccagcg cgttcagcac gtacttcgac agcagatatt taatctcatc gagcatgatg
    10141 ctcgcgtgcg gccaccaccg gaactcgtcc cgcgcaatga tgatttgcgc gcagcgggca
    10201 aacagcatct tctgcacccg gatcaccgcc tcatcgtcca tcgagccgat gacgtacagg
    10261 cagccgccgc cgttgattat ccccgccacg tcgatgccgc tgtccgtctg aaggacgctc
    10321 aggtcggcca cgttctccag cttggtgatc agccccttca cgtccttttt cagggcctcc
    10381 ggcagtgcgt acgccgccgc cagcacctgc tgaatatttg ccccctgcgg aaactgctcc
    10441 gcaatcagct tcgccgcttt ctgctcgctg atgcggtagt ggtccgccac gtcgccggtc
    10501 tcactcaggt taaatccggc aaccagaaga ttcttcaggg cgtactggtc gagatcgcgg
    10561 aacagattca gctgcgccgg tttccccggc cgcagatcca gcagggtgaa gtccgggcag
    10621 tgcgcccgga agacgtgcgg cgcccacgcg tcccctttcg ggtcgaagta caccaccgcg
    10681 tccccgaaat cgcgtacgca gcgaatgagc gcgttggtgg ccatcacgct cttgccgctc
    10741 ccgggcaggc cgaggatctg aatgttggtc ttgcgccagg tatcacgcgg gaccagcacc
    10801 ggctggccct gctcatcctt acccagaaac accgcctgct gtttccgggc ctgctccgtg
    10861 gaaaaatcgg ccagcgcgtt aaccggtacc ggctccccga aatgcaggtg gcggacgtcg
    10921 gtcatttctc cggtccgctg gctgctgcgc cggctctcct gattcagttt cgcgttcacc
    10981 gcctcgtcag cggccgcaaa atgcccgtca agcagcagga acgccagcag ccgcgccagg
    11041 aggacgcctg cgacaagacc tccggcacac caggcaatcc cgaacaggtt agccggctgc
    11101 agcaggtaat aatctgcgtt cgcgtagagg tccggacgca ggacggtttc tccccagctc
    11161 tcgataaaca cggcgacaac cagccccccc agaagggcca gaccggcagc gccggtcagc
    11221 cagtatgcgt agttccagac atgtcgccgt gccctgacca tccgcccggg cgaatccagc
    11281 gaggccagca ccgaaaatgg cgcagtccgg ttattatcgc gcttatagcg atcggcatac
    11341 tcccgccgct ccttatgact cagccccacg aatcgcatcc gcccggccca gagttctgcc
    11401 agaccgaaaa acaggccgat aaccagaaaa aaaccggcca gactgtaata ctcccggtac
    11461 agccagctga cccacgggta aatctgccgg gccagctccg ggatatgcca ctgccaggca
    11521 agcgaatccg tattaaacat gcaatctctc tccttcagac gacgggttta ccggcgtgtt
    11581 gtgcagcccc tgctgcacaa gccgcggaca tgccccaagg catggcggcg gcggtcacgt
    11641 ccgtggtgat tgcgttcagc tttcaccgcg gaggccagcc tctttccgcc ccatgcggaa
    11701 aaggcgcagc cccggaggaa caacgagcac gccccagcgt gtgagaggaa aaggcggcac
    11761 cccagtgcgg tcttttccgg tgttccggcc cccgtcagag ttcgccatag cgagcggctg
    11821 accacccgca aaatcaggag cagctttgct gcttcgtcag gtgcgtccag caccgtggat
    11881 tttgtgggtg gtcaggcgtg agcctgacgg gggccgggtg aatttctggc gtgaccggcg
    11941 tccagccgga cagagccaga aagaggggga aagacagtgc gtcggtggcg tccagccagc
    12001 gtaagcaggg gatttccccc tgaagtcagt cacgccccag cggggatgac gtgcatttct
    12061 ccgcaaggag aaaaaagcaa cagagatagg ttaacgtaaa atattcagca gaaaaccgac
    12121 agacttcacc tatctgaata atttatcggc agggaagaga aaggcggtaa cacggagcaa
    12181 tatgtcacat ccacgcgtcc ggtgaccgtc ttgcaggtgc cggggaaatt gcgcctcctc
    12241 gctcactcgg tcgctacgcg ccggccgtga gactgcggcg ggcgctgcga actctcgcaa
    12301 agttacccac agattccgtg gataagcagg gaacgagcat gtgaggcaaa acggcagggc
    12361 cgcgccggtg gcgtttttcc ataggctccg ccctcctgcc agagttcaca taaacagacg
    12421 ctttttcggt gcatctgtgg gagccgtgag gctcaaccat gaatctgaca gtacgggcga
    12481 aacccgacag gacttaaaga tccccaccgt ttccggtagg tcgctccctc ttgcgctctc
    12541 ctgttccgac cctgccgttt accggatacc tgtccgccgt tctcccttgc gggaagtgtg
    12601 gcgctttctc atagctcaca cactggtatc tcggcccggt gtaggtcgtt cgctccaagc
    12661 tgggctgtaa gcaagaactc cccgttcagc ccgactgctg cgccttatcc ggtaactgtt
    12721 cacttgagtc caacccggga aagcacgcaa aacgccactg gcag
//
