LOCUS       NZ_CP134004            12944 bp    DNA     linear   CON 21-OCT-2023
DEFINITION  Klebsiella pneumoniae strain AMR200031 plasmid unnamed6.
ACCESSION   NZ_CP134004
VERSION     NZ_CP134004.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37176067
            Assembly: GCF_032829175.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 12944)
  AUTHORS   Lai,Y.-C.
  TITLE     Diversification of ST11 KL64 Klebsiella pneumoniae in Taiwan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12944)
  AUTHORS   Tang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) Microbiology and Immunology, China Medical
            University, No. 91, Xueshi Road, Taichung 40402, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/21/2023 13:11:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,998
            CDSs (total)                      :: 5,874
            Genes (coding)                    :: 5,749
            CDSs (with protein)               :: 5,749
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 49 of 125
            Pseudo Genes (incomplete)         :: 73 of 125
            Pseudo Genes (internal stop)      :: 14 of 125
            Pseudo Genes (multiple problems)  :: 11 of 125
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP134004.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..12944
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="AMR200031"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed6"
                     /geo_loc_name="Taiwan: Kaohsiung"
                     /lat_lon="24.24 N 120.54 E"
                     /collection_date="2019-06-04"
                     /collected_by="Kaohsiung Medical University Chung-Ho
                     Memorial Hospital"
     gene            complement(87..1067)
                     /locus_tag="REM89_RS29745"
                     /old_locus_tag="REM89_29745"
     CDS             complement(87..1067)
                     /locus_tag="REM89_RS29745"
                     /old_locus_tag="REM89_29745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019445.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element ISKpn26 family transposase"
                     /protein_id="WP_000019473.1"
                     /translation="MSHQLTFADSEFSTKRRQTRKEIFLSRMEQILPWQNMVEVIEPFY
                     PKAGNGRRPYPLETMLRIHCMQHWYNLSDGAMEDALYEIASMRLFARLSLDSALPDRTT
                     IMNFRHLLEQHQLARQLFKTINRWLAEAGVMMTQGTLVDATIIEAPSSTKNKEQQRDPE
                     MHQTKKGNQWHFGMKAHIGVDAKSGLTHSLVTTAANEHDLNQLGNLLHGEEQFVSADAG
                     YQGAPQREELAEVDVDWLIAERPGKVKTLKQHPRKNKTAINIEYMKASIRARVEHPFRI
                     IKRQFGFVKARYKGLLKNDNQLAMLFTLANLFRVDQMIRQWERSQ"
                     /name="IS5-like element ISKpn26 family transposase"
     gene            <1136..1528
                     /locus_tag="REM89_RS29750"
                     /old_locus_tag="REM89_29750"
                     /pseudo=""
     CDS             <1136..1528
                     /locus_tag="REM89_RS29750"
                     /old_locus_tag="REM89_29750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525580.1"
                     /GO_function="GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]; GO:0003677 - DNA binding [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /name="3'-5' exonuclease"
     gene            1581..1976
                     /locus_tag="REM89_RS29755"
                     /old_locus_tag="REM89_29755"
     CDS             1581..1976
                     /locus_tag="REM89_RS29755"
                     /old_locus_tag="REM89_29755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015493087.1"
                     /translation="MENMMKTMREHFPTRTVVIEQSLDMFGVKRNEDGTLLFPRHSDKR
                     FPIARIRKRLAAYGWRGERLNKEVARIVAGPDIYAERNEYNIIFPYGWEKKQLTREMRR
                     MFPRKTGRRVLIMLDELHHFSDLREKP"
                     /name="hypothetical protein"
     gene            1973..2584
                     /locus_tag="REM89_RS29760"
                     /old_locus_tag="REM89_29760"
     CDS             1973..2584
                     /locus_tag="REM89_RS29760"
                     /old_locus_tag="REM89_29760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008455304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2913 family protein"
                     /protein_id="WP_015493086.1"
                     /translation="MTLTEKSGHLAWCALVALALARQEGGVLSPAQENLFLIRWLATAL
                     KQRRFPREINPDIEWLLKQRRQLGVSAKLASKLNYLWRSCTGELSEQNDLFRLTYALET
                     AKDMHWNYRLLSDREWSGRNAVALSAGVNGIYLSRANLDVAFDDSGRQINPLTARLTGN
                     VVGVMKVFNRCGWQAEPESGASLPHQYSLMAGQGVPGKGD"
                     /name="DUF2913 family protein"
     gene            complement(2581..3531)
                     /locus_tag="REM89_RS29765"
                     /old_locus_tag="REM89_29765"
     CDS             complement(2581..3531)
                     /locus_tag="REM89_RS29765"
                     /old_locus_tag="REM89_29765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011147726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="WP_015493085.1"
                     /translation="MKKKPGTPTPHDATFRQFLANPDVARDFMMLHLPAELRALCDLTT
                     LKLESGTFVEDDLRQYVSDILWSMKTTTGDDGYIHLLLEHQSSADKNMAFRQLRYAVAA
                     MQRHLEAGHKKLPLVIPVLFYHGSRSPYPFSTNWLDCFSNPEQAGKVYTTGFPLVDVTV
                     IDDDEIMNHRRMAALTLLMKHIRQRDMMELLDRLPRVMVEWISPEQVRVLINYMVNAGE
                     APAPEFMRALAERLPQHEDELMTIAQQLEQIGIEKGLQQGLQQGLQLGEQRGIEKGERE
                     ATLKIARTMLQNGIDRNTVMKMTGLTEDELAQIRH"
                     /name="Rpn family recombination-promoting nuclease/putative
                     transposase"
     gene            complement(3678..3878)
                     /locus_tag="REM89_RS29770"
                     /old_locus_tag="REM89_29770"
     CDS             complement(3678..3878)
                     /locus_tag="REM89_RS29770"
                     /old_locus_tag="REM89_29770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_040219232.1"
                     /translation="MAERISQREQELLEEFLCTVLDDFSKGSITHHQAVSGIATLYTAA
                     TEGRREEALEYLREGRKLLRQ"
                     /name="hypothetical protein"
     gene            complement(3932..4564)
                     /locus_tag="REM89_RS29775"
                     /old_locus_tag="REM89_29775"
     CDS             complement(3932..4564)
                     /locus_tag="REM89_RS29775"
                     /old_locus_tag="REM89_29775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497474.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_022652286.1"
                     /translation="MFVFGYLRASTSEQNASRAKNALKAFANQNGHRIAGWYIDNVSGT
                     TMNRPELIRLLGDAEPGDVILIEQVDRLTRLDDAGWETLRKQITDKQLAIVSLDLPTSH
                     LALSSTVSDDFTRSMLKAVNGMMLDMLAAIARKDYEDRRRRQAEGICKAKTEGKYRGRV
                     ADAQKHELIRTLRLAHGKSLRETARLAGVSKMTVIRVCNGNHKQDTD"
                     /name="recombinase family protein"
     gene            4927..6132
                     /locus_tag="REM89_RS29780"
                     /old_locus_tag="REM89_29780"
     CDS             4927..6132
                     /locus_tag="REM89_RS29780"
                     /old_locus_tag="REM89_29780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="WP_015493083.1"
                     /translation="MKRDYGSVGTIALRASALLQAMSRDIEEQRKEFNLTDYHQTYTRN
                     AVAKLPKLSRRIVELAMKEMEEDGYIFNKKQIGNVEQYALTIKNVIDIYAHRQIPKYRD
                     IHKEPYVIFVVNLKGGVSKTVSTVTLAHALRVHQDLLRHDLRILVIDLDPQASSTMFLD
                     HTHSIGTVLETAAQAMLNDLDVETLREAVIRPTIIPGVDVIPASIDDGFVASQWESLVA
                     EHLPGLKPSEVLRKTIIDRIAGDYDFVFIDTGPHLDPFLLNGLAASDLLLTPTPPAQVD
                     FHSTLKYLTRLPEMLERLEDEGVEPRLSASIGFMSKMTNKRDHETSHSLAREVYASNIL
                     DSSLPRLDGFERCGESFDTIISANPVSYPGSAEALKKARTEAERFTKAVFDRIEYIRGE
                     SK"
                     /name="AAA family ATPase"
     gene            6129..7100
                     /locus_tag="REM89_RS29785"
                     /old_locus_tag="REM89_29785"
     CDS             6129..7100
                     /locus_tag="REM89_RS29785"
                     /old_locus_tag="REM89_29785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="WP_016479949.1"
                     /translation="MKKVLARGRVLGNSNSEFARMLEGDGDVKTFTLKSGKQAKFVKTV
                     VLSGEIESKTFVDASVNGRDQAMLTRESVSDISRTIKLQQFFPAIGREVNGRIEILDGT
                     RRRAACIFNNVKFEILVTKDDISLADARQLAQDIQTAREHSLRELGKRLEVTYGTSMTK
                     EEIALKENLSPAKVTRAFQAAAVPDEMVAVFPVINDISLSDYQFLLKLAEEANNKQTSV
                     TELMEKVQHRLKTMPDYPAIDKSKILAAIRVESKSLTTRPTRTVQTEKLREFSDRNQFA
                     RKKTDSKKRLVVYEFSRISAEAQSEIDDAIKRILKRLPESSE"
                     /name="ParB family protein"
     gene            complement(7236..8507)
                     /locus_tag="REM89_RS29790"
                     /old_locus_tag="REM89_29790"
     CDS             complement(7236..8507)
                     /locus_tag="REM89_RS29790"
                     /old_locus_tag="REM89_29790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="WP_015493081.1"
                     /translation="MFALADVNSFYASCEKVFRPDLRNRPVVVLSNNDGCVIARSAEAK
                     RLGIKMGVPWFQLKTAQFPEPVITFSCNYELYASMSNRVMSHLEELAPRVEQYSIDEMF
                     LDIRGIDSCINYEDFGRQLREHVRSGTGLTIGVGMGPTKTLAKSAQWASKEWRQFGGVL
                     ALTSGNPKRTDKLLSRQPVEEIWGVGRRISKTLNTMGITTALQLARANPVFIRKNFSVV
                     LERTVRELNGESCISLEEAPPPKQQIVCSRSFGERVTTYEAMRQAVCQYAERAAEKLRG
                     ERQFCRHIAVFVKTSPFAVNEPYYGNVASEKLMTPTQDTRDIIAAAVKALDRIWVNGHR
                     YAKAGCMLNDFTPTGVSQLNLFDDTQPRSNSNQLMKVMDGINHSGLGKVWFAGRGIAPE
                     WQMKREMLSPAYTTRWSELPVAKL"
                     /name="Y-family DNA polymerase"
     gene            complement(8507..8929)
                     /gene="umuD"
                     /locus_tag="REM89_RS29795"
                     /old_locus_tag="REM89_29795"
     CDS             complement(8507..8929)
                     /gene="umuD"
                     /locus_tag="REM89_RS29795"
                     /old_locus_tag="REM89_29795"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000600201.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="WP_015493080.1"
                     /translation="MILFVTPASEPALSTAPLFTERCPAGFPSPAADYTEDELDLNAYC
                     IRRPSATYFVRAIGDSMKDMGLHSGDLMVVDKAEKPMQGDIVIAETDGEFTVKRLQLKP
                     RIALLPMNPAYPTLYPEELQIFGVVMAFIHKTRGTN"
                     /name="umuD"
     gene            complement(9109..9780)
                     /locus_tag="REM89_RS29800"
                     /old_locus_tag="REM89_29800"
     CDS             complement(9109..9780)
                     /locus_tag="REM89_RS29800"
                     /old_locus_tag="REM89_29800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004113322.1"
                     /GO_function="GO:0003697 - single-stranded DNA binding
                     [Evidence IEA]"
                     /GO_process="GO:0006974 - DNA damage response [Evidence
                     IEA]; GO:0018142 - protein-DNA covalent cross-linking
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family protein"
                     /protein_id="WP_015493079.1"
                     /translation="MCGRFTQYNSREEYLAFLGEEAERDIPYDPEPVGRYNVAPGTRVL
                     LLSERRSQLHLDPVHWGYAPEWWNKPALINARSETAAGSRMFRPLWEHGRAIVFADGWF
                     EWKQQNGVKQPFFIYRKDGNPLLLAAIGKPPFENGNDQEGFLIVTAAADEELLDIHDRR
                     PLVYSPDAARKWLSENTTGKEAEDIAREGSLPAEDFTWHPVSRAVGNPGNQGRELIEPL
                     T"
                     /name="SOS response-associated peptidase family protein"
     gene            10139..10816
                     /locus_tag="REM89_RS29805"
                     /old_locus_tag="REM89_29805"
     CDS             10139..10816
                     /locus_tag="REM89_RS29805"
                     /old_locus_tag="REM89_29805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="WP_002211749.1"
                     /translation="MSRFIQGNCVHIMSGFPDNAVDFILTDPPYLVGFRDRQGRTIAGD
                     KTDEWLQPACHEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKTYTSK
                     AAYVGYRHECAYILAKGRPPLPQNPLNDVIAWKYSGNRHHPTEKPVTSLQPLIESFTHP
                     GAIVLDPFAGSGSTCVAALQAGRRYIGIELLEQYHRAGQQRLAAVRRAMQYPAANDEFP
                     EAA"
                     /name="DNA methylase"
     gene            10816..11037
                     /locus_tag="REM89_RS29810"
                     /old_locus_tag="REM89_29810"
     CDS             10816..11037
                     /locus_tag="REM89_RS29810"
                     /old_locus_tag="REM89_29810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015493077.1"
                     /translation="MNYAGHEKLRAEVAEVANSMCDLRATLNGMEHRYRFDSDVLAERL
                     TRQTLFRINALFMAAYNEMLELDACFKD"
                     /name="hypothetical protein"
     gene            11048..11467
                     /locus_tag="REM89_RS29815"
                     /old_locus_tag="REM89_29815"
     CDS             11048..11467
                     /locus_tag="REM89_RS29815"
                     /old_locus_tag="REM89_29815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="WP_015493076.1"
                     /translation="MYGTCETLCRLLREQYPAETPLNLIVWSPADIEALADGMEYAVSE
                     QDTRAVLARMDAIPEEQRLESGVSAGAVMDLIEQVKEAVPAVMVPADLLETLLTTAEQA
                     LWHREWTARDCNHPVPESVTRRLADAAKVRALLKK"
                     /name="DUF1380 family protein"
     gene            11521..12300
                     /locus_tag="REM89_RS29820"
                     /old_locus_tag="REM89_29820"
     CDS             11521..12300
                     /locus_tag="REM89_RS29820"
                     /old_locus_tag="REM89_29820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015493075.1"
                     /translation="MQETTTLNALVMRRARDLIADYGWPDHTDVDQRDPVNKPGWISIY
                     VRLDAANIVHLLPLLCTGDVPADLQSAMTKISGTPAQIILSGSRYADAPQLPEDGTQIA
                     FPWAGEWLTEPEIQAVTDCLSRAVRDVSRQVWEDARRIKAALNTRGETLFYRQTRNFRL
                     VVKENDMPCWLDDDDNLPVVLDAILNKGARYSSVEFFVISDKVDQILACGQMCDVLRIP
                     GEPPRRWMDLTLLHEVMAEARAEISLVRNALSAIRPV"
                     /name="hypothetical protein"
     gene            12705..>12944
                     /locus_tag="REM89_RS29825"
                     /old_locus_tag="REM89_29825"
     CDS             12705..>12944
                     /locus_tag="REM89_RS29825"
                     /old_locus_tag="REM89_29825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="WP_317085477.1"
                     /translation="MRTTTTTPAVYVGTYHKYNCGSIFGKWFDLTEFDGREDFYEACQA
                     LHADEWDAEFMFQDWEGIPSQFVSECSIDWDFIAA"
                     /name="antirestriction protein ArdA"
ORIGIN
        1 atctgggccg atttttttcc cgcaaacaca tcgaatcagc ctatttaggc tattttttcc
       61 accatttctg gcgttatttc cggtttttac tgagatctct cccactgacg tatcatttgg
      121 tccacccgaa acaggttggc cagggtgaat aacatcgcca gttggttatc gtttttcagc
      181 agccctttgt atctggcttt cacgaagccg aactgccgct tgatgatgcg aaacgggtgc
      241 tccaccctgg cacggatgct ggctttcatg tattcgatgt tgatggccgt tttgttcttg
      301 cgcggatgct gcttcaaggt ttttaccttg ccgggacgct cggcgatcag ccagtccaca
      361 tccacctcgg ccagctcctc gcgctgtggc gctccttggt agccggcatc ggctgagaca
      421 aattgctcct ctccatgaag cagattaccc agctgattga ggtcatgctc gttggccgcg
      481 gtggtgacca ggctgtgggt caggccactc ttggcatcga caccaatgtg ggccttcatg
      541 ccaaagtgcc actgattgcc tttcttggtc tgatgcatct ccggatcgcg ttgctgctct
      601 ttgttcttgg tagagctggg tgcctcaatg atggtggcat ccaccaaagt gccttgggtc
      661 atcatgacgc ctgcttcggc cagccagcga ttgatggtct tgaacaattg acgggccagt
      721 tgatgctgct cgagcaggtg gcggaaattc atgatggtgg tgcgatccgg cagggcgcta
      781 tccagggata atcgggcaaa caggcgcatg gaggcgattt cgtacagggc atcttccatg
      841 gcaccgtcgc tcaggttgta ccaatgctgc atgcagtgaa tacgcagcat ggtctccagc
      901 ggatagggcc gtcggccatt gcccgccttg ggataaaacg gctcgatgac ttccaccatg
      961 ttttgccatg gcagaatctg ctccatgcgg gagaggaaaa tctcttttcg ggtctgacgg
     1021 cgcttagtgc tgaattcact atcggcgaag gtgagttgat ggctcatgat gtccctctgg
     1081 gatgcgctcc ggatgaatat gatgatctca tatcaggaac ttgttcgcac cttccatcag
     1141 tgacgatgat ctggtcagtg cgccatcgtg gcccgatatt gcgcagcagc tgcagcacca
     1201 tatcggccgg cgcccgctgg ttattttcaa tgctgagttt gatacgcgca ttctgaagca
     1261 gacagcggca gcgcataacg accgtgccag ctggctggac tctctgacgg tgtactgcgc
     1321 catgcgactt gcagcggggt attacggccc caccaatcgc tacggtacca tatcgctttc
     1381 cggcgctgtc agtcaggctg ggctgagctg ggccggggag gcacactccg ccgtcactga
     1441 cgcagttatg acggctcggg tggtaaacaa tattgccgga tactggcgtg agatccagtg
     1501 tgaaatgaat gatggcgcag ggagataacg ctgctctctg gctggtcaga cagctgcagg
     1561 tattaattca ggagtctgga atggaaaata tgatgaaaac tatgcgtgag cattttccca
     1621 cgagaacagt ggtgatcgag cagtctctgg atatgtttgg cgtcaaacga aatgaggacg
     1681 ggacgttact cttcccccgt cactccgata aacgttttcc cattgcacgt atccgtaagc
     1741 gactggcggc ttatggctgg cgaggcgaac gtctgaataa ggaggtagcg cgtatcgtcg
     1801 cgggccctga tatttacgct gagagaaatg aatacaacat tattttccct tacggatggg
     1861 agaagaagca gctgacgcgt gagatgcggc gaatgtttcc ccgtaagacg ggccgacgtg
     1921 tgctgattat gctggatgaa ttacatcatt tttctgactt gcgggagaag ccgtgacgct
     1981 gacagaaaaa tcgggccatc tggcatggtg tgccctggtg gcgcttgcgc tggccaggca
     2041 ggagggtggc gttctctcgc cggcacagga aaatctcttt ctcatccgct ggctggcgac
     2101 tgcgctgaag caacggcgtt tcccccggga gataaatccg gacatcgagt ggctgttgaa
     2161 acagaggcgc cagctgggcg tcagcgcgaa gctggccagc aagctgaatt acctctggcg
     2221 ttcctgcacc ggcgagttgt ccgagcagaa cgacctgttc cggctgacgt atgctctgga
     2281 aacagcgaaa gatatgcact ggaactaccg tctgctgagc gatcgggaat ggtcaggccg
     2341 aaatgctgtg gcgctcagtg cgggcgtgaa cggcatttat ctctcacggg ccaacctcga
     2401 tgtcgctttt gacgacagcg gccgacagat aaatccgctt acagcacgtc tgacgggtaa
     2461 cgtagtgggg gtgatgaagg tgtttaatcg ctgtggctgg caggcagagc ctgagtctgg
     2521 tgcctccctg ccccaccagt attctctgat ggccggacag ggagtgccag gaaaggggga
     2581 ttagtggcgg atctgcgcta gttcgtcttc agtcagaccg gtcattttca tgacggtatt
     2641 gcggtcaatt ccgttctgca gcatggttcg tgcaatttta agggtggctt cgcgctcgcc
     2701 tttttcaata ccgcgttgtt cacctaactg caggccctgc tgcagaccct gctgcaggcc
     2761 cttctcgatg ccaatctgtt caagctgttg tgcgatggtc atcagttcat cctcgtgttg
     2821 cggcagacgc tctgccagcg cccgcataaa ttcaggtgca ggcgcttcac ctgcatttac
     2881 catgtagttt atcagaacgc gcacctgttc gggggaaatc cattccacca ttacccgggg
     2941 tagcctgtcc agcagttcca tcatgtcccg ctggcggata tgtttcatca gcagggtcag
     3001 tgcggccatc cgtctgtggt tcattatctc atcatcatcg atgacggtga catcaaccag
     3061 cgggaatcct gtcgtgtata cttttccggc ctgttccggg ttactgaaac aatccagcca
     3121 gttcgtggag aacgggtacg gactccggct gccgtggtaa aacagaaccg ggatgaccag
     3181 cggcagtttt ttatgacccg cttcaagatg acgttgcatg gcggcgacag catatctgag
     3241 ctgacgaaag gccatatttt tatccgccga actctggtgt tccagaagca gatggatgta
     3301 cccgtcatcg ccggtggtgg ttttcatgct ccagagaatg tcgctgacgt actgacgtaa
     3361 gtcgtcctca acgaaggtac cggactccag cttcagcgtg gtcaaatcgc acagcgcgcg
     3421 taattcagcc gggagatgta acatcatgaa gtcacgggcg acatcgggat tcgccaggaa
     3481 ctggcgaaat gtcgcgtcat ggggtgtggg tgtacctggt ttcttcttca tcagtcctgg
     3541 gctctgaaaa atgacccgga gatgttatca cagcccggga aaagctaata ttggcggtga
     3601 acggaattgc cattccttca ggggaccgtt ccggggtggt tcggtcccct tcctaaacca
     3661 tctttactca tcatcaatta ctgccttagc aactttcttc cctctctcag atattctaaa
     3721 gcctcttctc tccgtccttc tgtggcagct gtatataatg tcgcgatacc gctgacagcc
     3781 tgatggtggg ttatactgcc tttactgaaa tcatccagaa cagtacaaag gaattcctcc
     3841 aacaactcct gttctctttg agatattctt tcagccatat tcgctccata agcttaattg
     3901 tttgttctga acctaacgtg aataagtatc gtcaatcagt atcctgctta tgattgccgt
     3961 tacagaccct gattactgtc atctttgata cgccagccag gcgggctgtt tcacgcagtg
     4021 attttccgtg ggccagacgc agagtacgta tcagttcatg tttctgtgca tcggccaccc
     4081 tgccccggta ttttccttca gttttcgctt tacagattcc ctctgcctgg cgacgtcgac
     4141 ggtcttcgta gtcttttctg gcaatagcgg cgagcatatc cagcatcatg ccatttacgg
     4201 ccttcagcat gcttcgggtg aagtcatcac ttactgtgct ggatagcgcc agatggctgg
     4261 ttggtaaatc aagactgacg atggccagct gcttgtcggt gatttgcttt ctgagtgtct
     4321 cccagcctgc atcatccagt cgagtaagcc tgtcgacctg ttcaatgaga atcacatcac
     4381 caggttccgc atcaccaaga agcctgatca gttcaggacg gttcattgtc gtaccggaga
     4441 cattgtcgat ataccagcca gcaatacggt ggccattttg gtttgcaaat gccttcagtg
     4501 cattttttgc ccggctggca ttctgctctg aagtggatgc ccggaggtaa ccaaaaacga
     4561 acatattttg tccttctggt aacttttaag tggtcttgct tatgatggta tcacttatgt
     4621 ggtaacaata atattcgccg gtattttttc gggtggtata tatagagcat accccaatgt
     4681 gacaggctga cgcgcccacg gagcttgtcc aacccctcgc ctgctcagat gcaagctctg
     4741 aacagactca cggttttttc tttgagtaca gcctcagttg tctttttgag ctacgtgata
     4801 ggtaggtatt aacgtacaat aatttaatgt cgtatcatta tatgttgtac gttattattt
     4861 attgtgtaca atggcgtgag cttctacgcc atcaatgtgc aaggattcca tctttagggg
     4921 acaaaaatga aacgcgatta cggtagtgtg ggtaccatag ctcttagagc aagcgcttta
     4981 cttcaggcta tgagtaggga tattgaggaa caaaggaaag aatttaatct gacagattat
     5041 catcagacat atactcgtaa cgctgtcgca aaactaccaa agctgagccg gcgtatcgta
     5101 gaactggcca tgaaagaaat ggaagaagac ggctatatat ttaataaaaa gcagattggt
     5161 aacgtcgaac aatatgctct gacgataaag aatgttatcg atatctacgc ccaccgacag
     5221 atacctaaat atcgcgatat ccataaagaa ccctacgtta ttttcgttgt aaaccttaaa
     5281 ggtggtgtat caaaaacagt cagtacagtt actcttgcac atgcgttgcg tgtacatcag
     5341 gacctgctgc ggcatgatct tcgcatcctt gtaatagacc tcgacccaca ggcctccagt
     5401 actatgttcc tggatcacac ccatagcatt ggtacagtcc ttgaaacagc tgctcaggca
     5461 atgctgaatg atctcgatgt tgaaacattg cgagaagcag ttattcgccc caccattatt
     5521 cctggtgttg atgtcatccc tgcttctatt gatgacggct ttgtggcgag ccagtgggag
     5581 tctttagtag cagaacattt accaggactt aaaccttctg aagtcctcag gaaaacaatt
     5641 attgaccgta tcgctggtga ctatgatttt gtcttcatag atactggccc ccatcttgat
     5701 ccgttccttc tgaatggtct ggcagcgagt gatttgcttc tgacccctac tccaccggca
     5761 caggttgatt tccattcaac ccttaaatat ctgacacgtt tgcctgaaat gctggagcgt
     5821 cttgaagacg aaggtgtgga accacgcctg agcgcgagta tcggtttcat gtcaaaaatg
     5881 actaataagc gtgatcacga aacttcgcat agcctggccc gcgaagtgta tgcaagtaat
     5941 attctggact cgtcactgcc acgtttggat ggattcgaac gatgtggtga atcttttgac
     6001 actattatta gtgctaaccc tgtttcatac cctggtagtg ctgaagcgtt gaaaaaggca
     6061 cgtactgaag cagaacggtt tacgaaagca gtttttgacc gcattgaata catccgggga
     6121 gagtctaaat gaaaaaggtt ttagctcgtg ggcgagttct cggtaatagc aattcagagt
     6181 ttgcccgtat gcttgaaggt gacggagatg tcaaaacgtt tactcttaaa tccggcaagc
     6241 aggccaaatt tgtcaaaacg gttgtattaa gcggagagat tgaatcaaag acgttcgttg
     6301 atgcatcggt caatggacga gatcaggcta tgcttacgcg tgagtccgtt agtgatattt
     6361 cccggacgat aaagctgcag caattcttcc cggctattgg acgggaggtt aatggacgaa
     6421 ttgagatatt agatgggacc cgacgccgtg ccgcctgcat atttaataac gttaaattcg
     6481 aaatcctagt aactaaagat gatatatcac tcgccgatgc acggcagtta gcgcaagata
     6541 tccagacagc tagagaacat agccttcgag agttggggaa acgacttgaa gtcacctatg
     6601 gaactagcat gacgaaagaa gagattgcgt tgaaagagaa tctctctccg gcgaaagtga
     6661 ctcgcgcgtt tcaggccgca gcagtgccag atgaaatggt tgcagtattc ccggtgataa
     6721 atgatatttc tttgtcggat tatcagtttt tgctaaaact ggctgaagaa gcaaacaaca
     6781 agcaaacatc cgtaacagaa ctgatggaaa aagttcaaca tcggttgaag accatgccag
     6841 attatccggc aattgataaa agcaaaattc tggcggctat cagggttgag agcaaatcgt
     6901 tgacaacccg ccctaccaga acggttcaaa ctgagaagct gagagaattt tcagatcgca
     6961 atcagtttgc cagaaagaaa actgattcaa agaagcgact ggttgtttat gagttttccc
     7021 gcatttccgc tgaggcacag tcggagattg atgatgcaat aaaacgtatt ctgaaaagac
     7081 ttcctgagtc aagtgagtaa gggaaaagga tgccggagag catccttttt ttatgttttt
     7141 caccatgccg atttcaatgt tattattatg attgtaaagg aaaattttaa attctttcac
     7201 actgaaatca ccaagttttt caaccttttc gtgactcata atttcgctac tggcagttca
     7261 gaccagcgag ttgtataggc cggtgaaagc atctcccgtt tcatttgcca ctcaggtgcg
     7321 atacctcgtc ccgcaaacca tactttcccc agaccggaat ggttaatacc gtccatgact
     7381 ttcatcagct gattactgtt gcttcggggc tgagtatcat cgaacaggtt caactgcgaa
     7441 accccggtag gagtgaaatc attgagcatg caacccgctt ttgcatatcg atggccattc
     7501 acccagatcc gatccagtgc tttcaccgct gcggctatga tgtccctggt atcctgcgtg
     7561 ggagtcatca acttctcact ggccacattc ccgtaatacg gttcgttcac cgcgaacggt
     7621 gacgtcttca caaatacagc aatgtgcctg cagaactgtc gctcgccgcg cagtttttca
     7681 gctgcacgct cagcatactg acaaactgcc tggcgcatgg cttcatacgt cgtgacacgc
     7741 tccccaaagg accggctgca gacaatctgc tgttttggcg gcggcgcttc ttcaagcgaa
     7801 atacagcttt ctccgttgag ttcacggacg gttctctcca gcacaacgct gaaattcttc
     7861 ctgataaata cagggttcgc gcgcgccagc tgcagtgccg tagtgatgcc catcgtatta
     7921 agcgtttttg agatcctgcg acctactccc cagatttctt ccaccggctg tcgtgagagc
     7981 agtttatccg ttcgctttgg attgcctgaa gtcagagcaa gcacgccccc aaactgccgc
     8041 cattccttag acgcccattg tgcgctttta gccagcgttt tggtcggccc catcccgacg
     8101 ccgattgtca gccccgtacc ggagcgcacg tgctcgcgca gctgccggcc gaagtcctca
     8161 taattgatgc agctgtctat accgcggata tccagaaaca tctcgtcgat gctgtactgc
     8221 tccacgcgcg gcgccagttc ttcgagatga gacataactc tgttcgacat agacgcatag
     8281 agctcgtaat tgcacgagaa cgtgatgacc ggctccggga attgtgccgt cttcagctgg
     8341 aaccagggga cgcccatttt aatacccagc cgtttcgctt cggcactgcg cgcgatgacg
     8401 cagccatcat tgttactgag caccacaacg ggcctgttac gcaggtccgg acgaaatact
     8461 ttctcacagc tggcgtagaa gctgttgacg tcagccaaag cgaacatcaa ttcgtacccc
     8521 gcgtcttatg tatgaatgcc atcaccacgc cgaatatctg cagttcttcc ggataaagag
     8581 tggggtaggc gggattcatc ggcagcaggg cgatacgggg tttaagctgc aggcgcttaa
     8641 cggtgaattc gccatctgtt tccgcgatga caatatcccc ctgcatgggt ttttcagctt
     8701 tgtcgacaac catgagatcg ccggagtgca ggcccatatc tttcatcgaa tcgccaatgg
     8761 cccggacaaa gtaggtcgcg ctggggcgac ggatacagta ggcgttcaga tccagttcgt
     8821 cttcggtata gtccgcggcc ggtgacggaa aaccagccgg acagcgctct gtgaaaagtg
     8881 gtgcggtgga cagtgctggc tctgatgcgg gagtgacaaa aaggatcatg gcatacctct
     8941 taaaatacct gtttttacat acagtgttat ttaagatatt tcccgtcctg caaggagaat
     9001 ttttcctgtt cctgccaggt tattgttctg gcagataaaa caggcagtgg gttatccaca
     9061 gaaacggtgc ataaccctgt gagtcgatgc cgggtatctc accgtctgct aagtcagtgg
     9121 ctctataagt tcccggccct ggttcccggg attgccaaca gcccggctga cggggtgcca
     9181 tgtgaaatct tcggcgggaa gactgccctc acgggcaatg tcttccgcct ctttaccggt
     9241 tgtgttttcg ctgagccatt tacgtgcggc atccggtgag tacacgagtg gtcgccggtc
     9301 gtgaatgtcc agcagttcct catccgcagc ggccgtgacg atcaggaatc cttcctggtc
     9361 attgccgttc tcaaaaggtg gtttcccgat ggcagcgagc aggagcggat ttccgtcctt
     9421 acggtaaatg aaaaacggct gtttcacgcc gttttgctgt ttccactcaa accagccgtc
     9481 agcaaaaaca atggcccgtc cgtgctccca gagtggcctg aacatccggc tgccggccgc
     9541 agtttcactt ctggcgttga tgagagcggg cttgttccac cactccggcg cataacccca
     9601 gtgaacagga tcaagatgca gctgcgagcg gcgttcactc agcagtaata cacgggttcc
     9661 gggcgcaacg ttgtagcggc cgacgggctc cggatcatac gggatgtccc gttctgcttc
     9721 ttctccgaga aaggccaggt actcctcgcg ggagttgtac tgcgtaaacc gaccgcacat
     9781 tatcgtttct ccggtgtaga tttcgcatgc gaaatctgtg ttttcatcag ttcagaaaat
     9841 aggccgtttt tcaggttcct gccatcgtgt ggccgccgtg ccgctactgt agccgggccg
     9901 cttcgaatgc aagggttcgc tctgccggtg tcctcacccg gtcgcagttt atttcaaagg
     9961 ctttacccgc tgtcttcccg tcgttttccg tctcaatttt cgccgtcaaa ccgtccagcc
    10021 agcgtgggct ttttcactgc gctgcggctt gcggcaccgc ccctgcattc tccacttttc
    10081 gcccggctgt cgtatcggca cggcgtaact ggcgccgctt tgagaaagga gaactactat
    10141 gtcccgattt atccagggta attgcgtcca tatcatgtcc ggctttccgg ataatgctgt
    10201 cgatttcatt ctcaccgacc cgccatatct cgtcggtttt cgtgaccgtc aggggcgtac
    10261 catcgccggc gataaaactg atgaatggct gcaacccgcc tgccatgaga tgtatcgcgt
    10321 gctgaaaaaa gacgcgctga tggtgagctt ctacggctgg aaccgcgtcg atcgctttat
    10381 ggccgcctgg aaaaatgcgg gattcagcgt tgtcggccac ctggtgttta ccaaaaccta
    10441 cacatcgaag gccgcatatg tgggctatcg ccacgaatgc gcctacatcc ttgcaaaagg
    10501 ccgtccgccc ctgccgcaaa acccgttgaa tgacgtaatt gcgtggaaat attcaggcaa
    10561 ccggcaccac ccgaccgaaa aacccgtaac cagcctgcaa ccgttgattg agtccttcac
    10621 acatcccggc gcgattgtgc tcgacccctt tgccggcagt ggctccacct gcgtggccgc
    10681 cctccaggct ggccgtcgtt acatcggtat tgaattgctt gagcagtatc accgggccgg
    10741 acagcagcgt ctggccgccg tccggcgcgc catgcagtac ccggccgcta acgacgagtt
    10801 cccggaggcc gcgtaatgaa ctatgcagga cacgaaaaac tccgcgcaga agtggcggaa
    10861 gtcgccaaca gcatgtgtga cctgcgggcg acgctgaacg ggatggagca ccgctatcgc
    10921 tttgactctg atgtgctggc cgaacgcctg acccgtcaga ccctttttcg catcaatgcc
    10981 ctgtttatgg cggcatacaa cgaaatgctt gagctggatg cctgctttaa ggactaagga
    11041 gaaaaacatg tacggaacct gtgaaaccct ctgccgcttg ttgcgtgagc agtatcccgc
    11101 agaaacccct ttgaacctca ttgtctggtc cccggcggat atcgaagcac tggccgacgg
    11161 aatggaatat gccgtttcgg agcaggacac aagggcggta ctggcgcgta tggacgccat
    11221 tccggaggaa caacggcttg agtcgggcgt gtctgccggt gcggtgatgg atctgattga
    11281 acaggtgaaa gaggccgttc cggcggtgat ggtgccggcg gacctgcttg aaaccctgct
    11341 gaccactgcc gaacaggcgt tatggcacag agaatggacc gcccgtgact gcaatcatcc
    11401 ggtcccggaa agcgttaccc gtcgcctggc tgatgcggcg aaagttcgcg ccttactgaa
    11461 aaaatgaaat caacacgccg cccgggcggc gtgtactgac cccgttcaga ggaacccatt
    11521 atgcaggaaa ccaccacgtt aaacgccctt gttatgcgcc gtgcgcgcga tttgattgct
    11581 gattatggat ggcctgacca taccgatgtt gatcagcgtg atccggtcaa taaaccgggc
    11641 tggataagca tttatgtccg tctggatgcg gcaaatattg ttcatctgct gcctttgctt
    11701 tgtaccggtg acgtacccgc agatctgcaa agtgccatga caaaaatatc cgggacgcca
    11761 gcgcagatta ttttatccgg cagccgctat gccgacgcgc cgcagcttcc ggaggacgga
    11821 acacagatag ctttcccctg ggccggggaa tggctgacgg agccggagat tcaggctgta
    11881 acggattgcc tgtcccgcgc cgtgcgggat gtatcccggc aggtatggga ggatgcgcgc
    11941 cggataaagg cggcactgaa cacgcgcggg gaaacgctgt tttatcgcca gacccgaaac
    12001 ttccgtctgg ttgtgaagga aaatgatatg ccctgctggc tggacgatga tgacaatttg
    12061 ccggtggtgc tcgatgccat cctgaacaaa ggggcacgct acagtagcgt cgagttcttt
    12121 gttatcagcg acaaagtgga tcaaatcctg gcatgtggcc agatgtgcga tgtgctgcgt
    12181 attccgggcg agcctccgcg ccggtggatg gatttaaccc tgttacatga ggtcatggca
    12241 gaagcgcgtg cagaaatcag tcttgtccgc aatgccctgt cagcaatccg gccagtttag
    12301 cgaacagcag ggggcggcgg ccccctgaat ctggcacgaa ggataaggat gtcgccggct
    12361 ccgcttttac ggctcttcct ccacgttgtt ccggcttcgc gttgagtaac gtcccgtgcg
    12421 gcctgcggca aacgccgtgc ctctactgta gccgggccgc ggctgatgca agggaagcgt
    12481 aaacgccgtc accttcaccc ggtcgcagtt tatttcatac gttatccgcc gtcttcccgt
    12541 cgttttccgg ctcagttttc gccgtcaaac cgtccagcct gctcctgctt ttttactgcg
    12601 ctgcggcttc ggtgacgccc ttgcatcctc cgcttttcgc ccggctgtcg tgtcggcacg
    12661 gcgtaagccg gaactcagac gacatcgact agggaggatt ttttatgcgc actacaacca
    12721 ccacacccgc tgtttacgtg ggcacgtatc acaaatacaa ctgcggcagc attttcggca
    12781 aatggtttga tctgacggaa tttgacggca gggaggattt ttacgaggcc tgtcaggcgc
    12841 tgcatgccga tgaatgggat gccgagttta tgtttcagga ctgggaaggt atcccgtcgc
    12901 agttcgtctc tgaatgctcc attgactggg attttatcgc cgct
//
