LOCUS       NZ_CP133866             9552 bp    DNA     circular CON 16-SEP-2023
DEFINITION  Enterobacter cloacae complex sp. EB5 plasmid unnamed3, complete
            sequence.
ACCESSION   NZ_CP133866
VERSION     NZ_CP133866.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37200060
            Assembly: GCF_031432835.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter cloacae complex sp. EB5
  ORGANISM  Enterobacter cloacae complex sp. EB5
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 9552)
  AUTHORS   Turton,J.F. and Turton,J.A.
  TITLE     Plasmid analysis using q20 nanopore sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9552)
  AUTHORS   Turton,J.F. and Turton,J.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-SEP-2023) HCAI, Fungal, AMR, AMU & Sepsis Division,
            United Kingdom Health Security Agency, 61 Colindale Avenue, London,
            London NW9 5EQ, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2023
            Assembly Method :: Flye v. 2.9.1-b1780; Medaka v. 1.7.2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 79x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/16/2023 11:12:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,908
            CDSs (total)                      :: 4,792
            Genes (coding)                    :: 4,671
            CDSs (with protein)               :: 4,671
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 49 of 121
            Pseudo Genes (incomplete)         :: 67 of 121
            Pseudo Genes (internal stop)      :: 25 of 121
            Pseudo Genes (multiple problems)  :: 17 of 121
            Pseudo Genes (short protein)      :: 1 of 121
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP133866.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9552
                     /organism="Enterobacter cloacae complex sp. EB5"
                     /mol_type="genomic DNA"
                     /strain="EB5"
                     /isolation_source="drain"
                     /host="Homo sapiens"
                     /db_xref="taxon:3073865"
                     /plasmid="unnamed3"
                     /geo_loc_name="United Kingdom: England"
                     /collection_date="2022-08"
                     /genotype="ST108"
     gene            594..917
                     /locus_tag="RHN82_RS24470"
                     /old_locus_tag="RHN82_24470"
     CDS             594..917
                     /locus_tag="RHN82_RS24470"
                     /old_locus_tag="RHN82_24470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_163063187.1"
                     /translation="MLTMWVTEGEHRRLLERCDGRPLAAWMRQTCLDEKPARTGKLPSL
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1126..>1560
                     /locus_tag="RHN82_RS24475"
                     /old_locus_tag="RHN82_24475"
                     /pseudo=""
     CDS             1126..>1560
                     /locus_tag="RHN82_RS24475"
                     /old_locus_tag="RHN82_24475"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1595..2086
                     /locus_tag="RHN82_RS24480"
                     /old_locus_tag="RHN82_24480"
     CDS             1595..2086
                     /locus_tag="RHN82_RS24480"
                     /old_locus_tag="RHN82_24480"
                     /inference="COORDINATES: protein motif:HMM:NF016710.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032629998.1"
                     /translation="MSSLLTLAKDLEQQSKAQQQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWFTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            2093..2308
                     /locus_tag="RHN82_RS24485"
                     /old_locus_tag="RHN82_24485"
     CDS             2093..2308
                     /locus_tag="RHN82_RS24485"
                     /old_locus_tag="RHN82_24485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137422.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2979..3221)
                     /locus_tag="RHN82_RS24490"
                     /old_locus_tag="RHN82_24490"
     CDS             complement(2979..3221)
                     /locus_tag="RHN82_RS24490"
                     /old_locus_tag="RHN82_24490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023316026.1"
                     /translation="MALVRSGNKSKIFEADTKFNSVFDTEYKPGETCEYEGIYKCTGCG
                     KEVTIAGGKTIPPQNHHQHTPAQGNIRWQLFVYAQ"
                     /name="hypothetical protein"
     gene            3408..3725
                     /locus_tag="RHN82_RS24495"
                     /old_locus_tag="RHN82_24495"
     CDS             3408..3725
                     /locus_tag="RHN82_RS24495"
                     /old_locus_tag="RHN82_24495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_023316025.1"
                     /translation="MQQDHEHDDCRIQFRDDWLYQFYKYGKANRKIPTAISASLGRKLD
                     MLNAADKVGDLRSPPGNRFENLNPPLTGYSSIRVNEKYRLIFKWGENGVEDLYLDPHDY
                     R"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            3928..4284
                     /locus_tag="RHN82_RS24500"
                     /old_locus_tag="RHN82_24500"
     CDS             3928..4284
                     /locus_tag="RHN82_RS24500"
                     /old_locus_tag="RHN82_24500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075993.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="WP_023316024.1"
                     /translation="MSQATRKPSTVGDIILYEYLEPSGLKINDLAEMLNVHRNSASALV
                     NNNRKLSTDMAFRLAKAFDTTAKFWLNIQQSVDLWEVTSSPRLQEEIDRVIPAHEIIER
                     KHIVDSSEKKHAIA"
                     /name="HigA family addiction module antitoxin"
     gene            complement(<4604..4726)
                     /locus_tag="RHN82_RS24505"
                     /old_locus_tag="RHN82_24505"
                     /pseudo=""
     CDS             complement(<4604..4726)
                     /locus_tag="RHN82_RS24505"
                     /old_locus_tag="RHN82_24505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487656.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            5377..5700
                     /locus_tag="RHN82_RS24510"
                     /old_locus_tag="RHN82_24510"
     CDS             5377..5700
                     /locus_tag="RHN82_RS24510"
                     /old_locus_tag="RHN82_24510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_163063187.1"
                     /translation="MLTMWVTEGEHRRLLERCDGRPLAAWMRQTCLDEKPARTGKLPSL
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            5909..>6343
                     /locus_tag="RHN82_RS24515"
                     /old_locus_tag="RHN82_24515"
                     /pseudo=""
     CDS             5909..>6343
                     /locus_tag="RHN82_RS24515"
                     /old_locus_tag="RHN82_24515"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            6378..6869
                     /locus_tag="RHN82_RS24520"
                     /old_locus_tag="RHN82_24520"
     CDS             6378..6869
                     /locus_tag="RHN82_RS24520"
                     /old_locus_tag="RHN82_24520"
                     /inference="COORDINATES: protein motif:HMM:NF016710.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032629998.1"
                     /translation="MSSLLTLAKDLEQQSKAQQQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWFTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            6876..7091
                     /locus_tag="RHN82_RS24525"
                     /old_locus_tag="RHN82_24525"
     CDS             6876..7091
                     /locus_tag="RHN82_RS24525"
                     /old_locus_tag="RHN82_24525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137422.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(7762..8004)
                     /locus_tag="RHN82_RS24530"
                     /old_locus_tag="RHN82_24530"
     CDS             complement(7762..8004)
                     /locus_tag="RHN82_RS24530"
                     /old_locus_tag="RHN82_24530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023316026.1"
                     /translation="MALVRSGNKSKIFEADTKFNSVFDTEYKPGETCEYEGIYKCTGCG
                     KEVTIAGGKTIPPQNHHQHTPAQGNIRWQLFVYAQ"
                     /name="hypothetical protein"
     gene            8191..8508
                     /locus_tag="RHN82_RS24535"
                     /old_locus_tag="RHN82_24535"
     CDS             8191..8508
                     /locus_tag="RHN82_RS24535"
                     /old_locus_tag="RHN82_24535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_023316025.1"
                     /translation="MQQDHEHDDCRIQFRDDWLYQFYKYGKANRKIPTAISASLGRKLD
                     MLNAADKVGDLRSPPGNRFENLNPPLTGYSSIRVNEKYRLIFKWGENGVEDLYLDPHDY
                     R"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            8711..9067
                     /locus_tag="RHN82_RS24540"
                     /old_locus_tag="RHN82_24540"
     CDS             8711..9067
                     /locus_tag="RHN82_RS24540"
                     /old_locus_tag="RHN82_24540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075993.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="WP_023316024.1"
                     /translation="MSQATRKPSTVGDIILYEYLEPSGLKINDLAEMLNVHRNSASALV
                     NNNRKLSTDMAFRLAKAFDTTAKFWLNIQQSVDLWEVTSSPRLQEEIDRVIPAHEIIER
                     KHIVDSSEKKHAIA"
                     /name="HigA family addiction module antitoxin"
     Typing          305..479
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.09"
                     /coverage="100.0"
                     /evalue="5.699999999999999e-63"
     Typing          5089..5262
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.0"
                     /coverage="100.0"
                     /evalue="1.21e-74"
     Typing          complement(3..378)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.974"
                     /coverage="97.0"
                     /evalue="4.37e-134"
     Typing          complement(4614..5122)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.51"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          complement(9167..9552)
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="81.752"
                     /coverage="100.0"
                     /evalue="5.25e-83"
     Typing          595..911
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.453"
                     /coverage="99.0"
                     /evalue="1.47e-59"
     Typing          908..2400
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.2"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5378..5694
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.453"
                     /coverage="99.0"
                     /evalue="1.47e-59"
     Typing          5691..7183
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.2"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aaaccgcggc cataacagcg gaatgacacc ggtaaaccga aaggcaggaa cgggggagcg
       61 cacgagggag ccaccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
      121 ccactgattt gagcgtcaga ttctgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
      181 cggctctggc gtggccttat cgcttccctg ttaagtatct tcctggcatc ccccaggaaa
      241 tttccgcccc gttcgtaagc ccgccccgct cgccgcagcc gaacgaccga gcgcagcgag
      301 tcagtgagcg aggaagcgga atatattctg tatcacatat tctcctgacg cgccgttgcc
      361 gccttttttc tcctgccata tgaggcactt actgatttgc gtatccgtgc cattacatat
      421 taagccagta tacactccgc tagcgctacg tgactggttc agggctgcgc cccgaaaccc
      481 gccaaaagcc actgacgcgc ctgcggcttg tccaaccccg cgccgaccgg gaaaggcttt
      541 cccgcccgtc ccggcgttat caggaggccg gtttgtcaga gaaacggaac aagatgctca
      601 ccatgtgggt gacggagggc gagcaccggc ggctgcttga gcgctgcgac ggcaggccgc
      661 tggcggcgtg gatgcggcag acctgcctgg acgaaaagcc tgcgcgcacc ggaaagctcc
      721 cctcactctc gccggcgctg ctgcgccagc ttgccggtat gggcaataac ctgaaccaga
      781 tagcccgccg tgttaacgcc ggcggcggca ccggccatga cagggtacag attgtggcgg
      841 cgctgatggc catcgatgcc gggcttgagc ggctgcggca cgccgttctt gagaaaggaa
      901 cggacgatga tcgttaaatt tcatccacgc ggtcgcggcg gtggcgccgg gccggtggac
      961 tatctgctgg gtaaagaccg gcagcgtgaa ggcgccagcg tcctgcaggg gaagccggaa
     1021 gaagtccggg agctgattga cgcgtcgcct tacgtgaaga aatacacctc cggcgtactg
     1081 tcgtttgcgg aggccgatct gcctcccggc cagcgggaaa aactgatggc cagcttcgag
     1141 cgggttctga tgcccggact cgataaagat cagtacagca ttctgtgggt ggagcacgcg
     1201 gacaaggggc gtctggagct gaactttctg atccccaata ccgaactgct gaccggcagg
     1261 cggctccagc cgtactacga ccgcgccgac cggccgcgca tcgatgcctg gcagaccatc
     1321 gtgaacggca gactggggct gcatgacccg aacgcgccgg aaaaccgccg ggcgctggtc
     1381 acgccgtccg gactgccgaa aacgaagcag gaggccactg aagccatcac gcgcggactg
     1441 ctgacccttg cttcgtccgg ggagctgaaa aaccgtcagg acgtcactga ggcgctggaa
     1501 cggtcaggtt ttgaggtggt gcgcaccacg aaaagcagta tcagcattgc cgacccggac
     1561 ggggggcgaa acatccgact gaagggagcc atctatgagc agtcttttaa cgctggcgaa
     1621 ggacttagag cagcaatcga aagcgcagca gcagagtacc ggcgagatgc tgaaagccgc
     1681 attcagcgag cacgagcagt ctgtcagagc ggaactgagc gcaagcgcga ggagaatcag
     1741 cgacgccatc ctcgcacacg agcagagtat gagcgaggcg atggagaaaa accggcggag
     1801 cgtactgcgg actgccgggc ggacatggtt caccatcctg atggtgtcag cgctgctgat
     1861 cggcacgagc gggagcatac tgtggtggca ggggcagcag ataaccgaca attacacgca
     1921 tcttcgtcag caggaagata ccctggcgaa aatgactgcc cggacgtggg gagtgcgcta
     1981 tcaggagagc agcgacggcc ggaggtttct tatcctgccg cccgggatgc aaaccgaagc
     2041 catcccctac gacgggacga catggatccg cctgaaacag gagtagcgga aaatgacaga
     2101 gctggaaaag cagttgctga gcgcattaga gcagctacag caggactact cgcaaaggct
     2161 ggacgaatgg gagagcgcct tcgcggaatg gcggagcatg tgtgggctta tgcaacggga
     2221 gaacgcggtg ctgaacgagc gcgtgtcgga cttgagcacg caggtgctga gtttaagcga
     2281 gcagctgcgc cgcttgtccg ggaactgaat gccatcgagc tccggcagag ggagaatgag
     2341 tatcagaaaa cactggagct ggagcgccag ccgcagaaaa cctaccgcgg gccgacactt
     2401 taaaccatcc aggcagctga caacccgcac gaagaggggc tcttatttct cccgctaaat
     2461 aggttcgtga tcaaaaaggt cacgacttat tcaggagcgg gaaatgaagc tgttaaatct
     2521 cattgaatca ttgcttaact gcctgtatct ggcggcggag ctgtgggact ggctggagaa
     2581 gcatttgtcg gtttttaagg cattggcgcg gattttagtg tgactttaac atccatgcat
     2641 ctggctgcat gactatgcac cagtgtaata ttcagcggct gcgtttctgg tgttttttcg
     2701 ttatgtctgt cgccactttc tcgcgtctgg caccggaaac gcagccaacc ccgtttcagc
     2761 ggtgcgccca acggatgtta tggtaaatca gtatcagctg cagatataaa aaagccgcca
     2821 ggctggcggc ttgctgttat tttcgtggct ttcctgcatc tgagcgtttt cctctgaccc
     2881 ttccgtcatt gttacggtca cgaggttgag ttccgccgcc tggtttagga acgacaacgt
     2941 gtgggttgag tggtcgacga tctgccattt tctatacctc actgagcata aacaaacaat
     3001 tgccaacgaa tgttcccttg cgcgggggta tgttggtggt ggttttgtgg tgggattgtt
     3061 ttcccgcctg cgatagttac ttctttaccg caaccggtac atttataaat cccttcatat
     3121 tcacatgttt ctcccggctt atattcagtg tcaaaaaccg agttaaactt cgtatctgct
     3181 tcaaaaatct tgcttttgtt tcctgagcga acaagagcca tgcaacctcc aaattatcat
     3241 ttattttgca cacccgcact gtgcatgagt gcattctaca gcatagccat tgattgcaca
     3301 agtgcactgt gctgttgtgc atttgatatg tttgctgtac tatatcggag gtaagaaggt
     3361 gatcgaaaaa attgccaagt tgcggcaaaa attgaggtaa aagaaaaatg cagcaagatc
     3421 atgaacatga tgattgccgc attcagtttc gagacgattg gctataccag ttttacaaat
     3481 atggtaaagc caatcgaaag attccaactg cgattagtgc ttcattaggt cgcaagctgg
     3541 atatgctgaa tgctgctgat aaagttggag atttgcggtc tcccccggga aaccgatttg
     3601 agaatctcaa ccctccattg acagggtatt catccatacg ggtgaatgag aagtaccgct
     3661 tgatatttaa gtggggtgaa aacggagtcg aggatttgta tctcgatcca catgattacc
     3721 gatagataac aggtcgtggc tgttatctta taaaacaagt aggtgaggcg ttccctcacc
     3781 tacattaaaa tgtcattaca aataatgatt aataaaaaaa atgacgtgac tatatttaag
     3841 ataattactc atcgctacag ctggtaatag acgaaatact tatcttcctc atctaacaga
     3901 gagaagtaaa aaatttagga gtaaagcatg tcccaagcaa ctagaaaacc atcaactgta
     3961 ggggatatta ttctttatga atatctcgaa ccaagtggtt taaaaatcaa tgacttggct
     4021 gaaatgttga atgtgcatag aaactctgcc agtgctttag ttaacaataa tagaaagctg
     4081 tctaccgata tggcttttag gttggctaaa gccttcgata ccactgcaaa gttctggcta
     4141 aatatacagc agtctgttga tttatgggag gttactagta gccctcgatt gcaagaagaa
     4201 atagacagag tgattcccgc tcatgaaatc atagaaagga aacacattgt tgatagttct
     4261 gagaagaaac atgctattgc atgatttttt ttaaaaaaat ataagttatc cctcaaaaag
     4321 aaggcttcta taactagaag ccttcttttt gagggataaa aacgctcatg ttaactttct
     4381 tcttacgatc ttcttgagat ccgtgctctg tacgcgtaat ctcttgctct gtaaacgaaa
     4441 aaaccgcctg gggaggcggt ttttcgaagg ttaagtcagt tggggaactg cttaacctgg
     4501 taactggctt cagcagagcg cagataccaa atactgtcct tccagtgtag ccgtagttag
     4561 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     4621 cagtggctgc tgccagtggc gttttgtcgt gtctttccgg gttggactca agtcgatagt
     4681 taccggaaca ggcgcagcgg tcggactgaa cggggggttc gtgcatacag tccagcttgg
     4741 agcgaactgc ctacccggaa ccgagtgtca ggcgtggaat gagacaaacg cggccataac
     4801 agcggaatga caccggtaaa ccgaaaggca ggaacaggag agcgcacgag ggagccatca
     4861 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccaccactg atttgagcgt
     4921 cagattctgt gatgctcgtc aggggggcgg agcctatgga aaaccggctc tgccgtggcc
     4981 ttatcgcttc cctgttaagt ttcttcctgg catcttccag gaaatttccg ccccgttcgt
     5041 aagcccgcac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     5101 cggaatatat cctgtatcgc atattctgct gacgcgccgt tgccgccttt tttctcctgc
     5161 cacatgaagc acttcactga tttccgtatc cgtgccaaca tattaagcca gtatacactc
     5221 cgctagcgct acgtgactgg ttcagggctg cgccccgaaa cccgccaaaa gccactgacg
     5281 cgcctgcggc ttgtccaacc ccgcgccgac cgggaaaggc tttcccgccc gtcccggcgt
     5341 tatcaggagg ccggtttgtc agagaaacgg aacaagatgc tcaccatgtg ggtgacggag
     5401 ggcgagcacc ggcggctgct tgagcgctgc gacggcaggc cgctggcggc gtggatgcgg
     5461 cagacctgcc tggacgaaaa gcctgcgcgc accggaaagc tcccctcact ctcgccggcg
     5521 ctgctgcgcc agcttgccgg tatgggcaat aacctgaacc agatagcccg ccgtgttaac
     5581 gccggcggcg gcaccggcca tgacagggta cagattgtgg cggcgctgat ggccatcgat
     5641 gccgggcttg agcggctgcg gcacgccgtt cttgagaaag gaacggacga tgatcgttaa
     5701 atttcatcca cgcggtcgcg gcggtggcgc cgggccggtg gactatctgc tgggtaaaga
     5761 ccggcagcgt gaaggcgcca gcgtcctgca ggggaagccg gaagaagtcc gggagctgat
     5821 tgacgcgtcg ccttacgtga agaaatacac ctccggcgta ctgtcgtttg cggaggccga
     5881 tctgcctccc ggccagcggg aaaaactgat ggccagcttc gagcgggttc tgatgcccgg
     5941 actcgataaa gatcagtaca gcattctgtg ggtggagcac gcggacaagg ggcgtctgga
     6001 gctgaacttt ctgatcccca ataccgaact gctgaccggc aggcggctcc agccgtacta
     6061 cgaccgcgcc gaccggccgc gcatcgatgc ctggcagacc atcgtgaacg gcagactggg
     6121 gctgcatgac ccgaacgcgc cggaaaaccg ccgggcgctg gtcacgccgt ccggactgcc
     6181 gaaaacgaag caggaggcca ctgaagccat cacgcgcgga ctgctgaccc ttgcttcgtc
     6241 cggggagctg aaaaaccgtc aggacgtcac tgaggcgctg gaacggtcag gttttgaggt
     6301 ggtgcgcacc acgaaaagca gtatcagcat tgccgacccg gacggggggc gaaacatccg
     6361 actgaaggga gccatctatg agcagtcttt taacgctggc gaaggactta gagcagcaat
     6421 cgaaagcgca gcagcagagt accggcgaga tgctgaaagc cgcattcagc gagcacgagc
     6481 agtctgtcag agcggaactg agcgcaagcg cgaggagaat cagcgacgcc atcctcgcac
     6541 acgagcagag tatgagcgag gcgatggaga aaaaccggcg gagcgtactg cggactgccg
     6601 ggcggacatg gttcaccatc ctgatggtgt cagcgctgct gatcggcacg agcgggagca
     6661 tactgtggtg gcaggggcag cagataaccg acaattacac gcatcttcgt cagcaggaag
     6721 ataccctggc gaaaatgact gcccggacgt ggggagtgcg ctatcaggag agcagcgacg
     6781 gccggaggtt tcttatcctg ccgcccggga tgcaaaccga agccatcccc tacgacggga
     6841 cgacatggat ccgcctgaaa caggagtagc ggaaaatgac agagctggaa aagcagttgc
     6901 tgagcgcatt agagcagcta cagcaggact actcgcaaag gctggacgaa tgggagagcg
     6961 ccttcgcgga atggcggagc atgtgtgggc ttatgcaacg ggagaacgcg gtgctgaacg
     7021 agcgcgtgtc ggacttgagc acgcaggtgc tgagtttaag cgagcagctg cgccgcttgt
     7081 ccgggaactg aatgccatcg agctccggca gagggagaat gagtatcaga aaacactgga
     7141 gctggagcgc cagccgcaga aaacctaccg cgggccgaca ctttaaacca tccaggcagc
     7201 tgacaacccg cacgaagagg ggctcttatt tctcccgcta aataggttcg tgatcaaaaa
     7261 ggtcacgact tattcaggag cgggaaatga agctgttaaa tctcattgaa tcattgctta
     7321 actgcctgta tctggcggcg gagctgtggg actggctgga gaagcatttg tcggttttta
     7381 aggcattggc gcggatttta gtgtgacttt aacatccatg catctggctg catgactatg
     7441 caccagtgta atattcagcg gctgcgtttc tggtgttttt tcgttatgtc tgtcgccact
     7501 ttctcgcgtc tggcaccgga aacgcagcca accccgtttc agcggtgcgc ccaacggatg
     7561 ttatggtaaa tcagtatcag ctgcagatat aaaaaagccg ccaggctggc ggcttgctgt
     7621 tattttcgtg gctttcctgc atctgagcgt tttcctctga cccttccgtc attgttacgg
     7681 tcacgaggtt gagttccgcc gcctggttta ggaacgacaa cgtgtgggtt gagtggtcga
     7741 cgatctgcca ttttctatac ctcactgagc ataaacaaac aattgccaac gaatgttccc
     7801 ttgcgcgggg gtatgttggt ggtggttttg tggtgggatt gttttcccgc ctgcgatagt
     7861 tacttcttta ccgcaaccgg tacatttata aatcccttca tattcacatg tttctcccgg
     7921 cttatattca gtgtcaaaaa ccgagttaaa cttcgtatct gcttcaaaaa tcttgctttt
     7981 gtttcctgag cgaacaagag ccatgcaacc tccaaattat catttatttt gcacacccgc
     8041 actgtgcatg agtgcattct acagcatagc cattgattgc acaagtgcac tgtgctgttg
     8101 tgcatttgat atgtttgctg tactatatcg gaggtaagaa ggtgatcgaa aaaattgcca
     8161 agttgcggca aaaattgagg taaaagaaaa atgcagcaag atcatgaaca tgatgattgc
     8221 cgcattcagt ttcgagacga ttggctatac cagttttaca aatatggtaa agccaatcga
     8281 aagattccaa ctgcgattag tgcttcatta ggtcgcaagc tggatatgct gaatgctgct
     8341 gataaagttg gagatttgcg gtctcccccg ggaaaccgat ttgagaatct caaccctcca
     8401 ttgacagggt attcatccat acgggtgaat gagaagtacc gcttgatatt taagtggggt
     8461 gaaaacggag tcgaggattt gtatctcgat ccacatgatt accgatagat aacaggtcgt
     8521 ggctgttatc ttataaaaca agtaggtgag gcgttccctc acctacatta aaatgtcatt
     8581 acaaataatg attaataaaa aaaatgacgt gactatattt aagataatta ctcatcgcta
     8641 cagctggtaa tagacgaaat acttatcttc ctcatctaac agagagaagt aaaaaattta
     8701 ggagtaaagc atgtcccaag caactagaaa accatcaact gtaggggata ttattcttta
     8761 tgaatatctc gaaccaagtg gtttaaaaat caatgacttg gctgaaatgt tgaatgtgca
     8821 tagaaactct gccagtgctt tagttaacaa taatagaaag ctgtctaccg atatggcttt
     8881 taggttggct aaagccttcg ataccactgc aaagttctgg ctaaatatac agcagtctgt
     8941 tgatttatgg gaggttacta gtagccctcg attgcaagaa gaaatagaca gagtgattcc
     9001 cgctcatgaa atcatagaaa ggaaacacat tgttgatagt tctgagaaga aacatgctat
     9061 tgcatgattt tttttaaaaa aatataagtt atccctcaaa aagaaggctt ctataactag
     9121 aagccttctt tttgagggat aaaaacgctc atgttaactt tcttcttacg atcttcttga
     9181 gatccgtgct ctgtacgcgt aatctcttgc cctgtaaacg aaaaaaccac cctggcaggt
     9241 ggtttttcga aggttaggta atcctgggga accccctaac cgtggtaaca gtcttgagcg
     9301 gacatgttac caaatttgtc ctttcagtgt agcctcacta aggccgccac ttcaagaact
     9361 cttgagacat tctcgcacat cctgtttgcc aatggccgtt gccaatggcg attagtcgtg
     9421 tctttccggg ttggactcaa gttgatagtt accggataag gcgcagcggt cggactgaac
     9481 ggggggttcg tgcatacagt ccatcctgga gcgaactgcc ttcccggaac tgagtgtcag
     9541 gcgtggaatg ag
//
