LOCUS       NZ_CP133865             9873 bp    DNA     circular CON 16-SEP-2023
DEFINITION  Enterobacter cloacae complex sp. EB5 plasmid unnamed1, complete
            sequence.
ACCESSION   NZ_CP133865
VERSION     NZ_CP133865.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37200060
            Assembly: GCF_031432835.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter cloacae complex sp. EB5
  ORGANISM  Enterobacter cloacae complex sp. EB5
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 9873)
  AUTHORS   Turton,J.F. and Turton,J.A.
  TITLE     Plasmid analysis using q20 nanopore sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9873)
  AUTHORS   Turton,J.F. and Turton,J.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-SEP-2023) HCAI, Fungal, AMR, AMU & Sepsis Division,
            United Kingdom Health Security Agency, 61 Colindale Avenue, London,
            London NW9 5EQ, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2023
            Assembly Method :: Flye v. 2.9.1-b1780; Medaka v. 1.7.2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 79x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/16/2023 11:12:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,908
            CDSs (total)                      :: 4,792
            Genes (coding)                    :: 4,671
            CDSs (with protein)               :: 4,671
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 49 of 121
            Pseudo Genes (incomplete)         :: 67 of 121
            Pseudo Genes (internal stop)      :: 25 of 121
            Pseudo Genes (multiple problems)  :: 17 of 121
            Pseudo Genes (short protein)      :: 1 of 121
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP133865.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9873
                     /organism="Enterobacter cloacae complex sp. EB5"
                     /mol_type="genomic DNA"
                     /strain="EB5"
                     /isolation_source="drain"
                     /host="Homo sapiens"
                     /db_xref="taxon:3073865"
                     /plasmid="unnamed1"
                     /geo_loc_name="United Kingdom: England"
                     /collection_date="2022-08"
                     /genotype="ST108"
     gene            complement(join(9774..9873,1..656))
                     /locus_tag="RHN82_RS24415"
                     /old_locus_tag="RHN82_24415"
     CDS             complement(join(9774..9873,1..656))
                     /locus_tag="RHN82_RS24415"
                     /old_locus_tag="RHN82_24415"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="WP_310399653.1"
                     /translation="MFSLKNMFSLNLQRSAISYDAICQNKPVIEFSPEGVINKASPLFL
                     STMGYRADEIIGHHHRIFCPPSLVSSPEYSQFWQRLARGESFSGKYLRLAKGNRSVWLE
                     ASYIPVSDRRGRVIRVIKIAADISERVHSALEQEAVVNAINRSMAIITFNPEGIVLEAN
                     ENFVNATGYKRDEIIGKHHRLFCAETLYKSDEYRHFWESLNQGEFFSGLFPRLNRQVCF
                     HALTVRETLCGSVQLITLSLTVMGSFIKL"
                     /name="PAS domain S-box protein"
     gene            complement(1552..1767)
                     /locus_tag="RHN82_RS24420"
                     /old_locus_tag="RHN82_24420"
     CDS             complement(1552..1767)
                     /locus_tag="RHN82_RS24420"
                     /old_locus_tag="RHN82_24420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021243037.1"
                     /translation="MTELEKQLLSVLEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1774..2265)
                     /locus_tag="RHN82_RS24425"
                     /old_locus_tag="RHN82_24425"
     CDS             complement(1774..2265)
                     /locus_tag="RHN82_RS24425"
                     /old_locus_tag="RHN82_24425"
                     /inference="COORDINATES: protein motif:HMM:NF016710.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_047720453.1"
                     /translation="MSSLLTLAKDLEQQSKAQQKSTGEMLKAAFSEHEQSVRAELNASA
                     RRISDAISAHEQSMSEAMEKNRRSVLLTAGRTWLTILMVSALLIATSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQAEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            complement(<2300..3004)
                     /locus_tag="RHN82_RS24430"
                     /old_locus_tag="RHN82_24430"
                     /pseudo=""
     CDS             complement(<2300..3004)
                     /locus_tag="RHN82_RS24430"
                     /old_locus_tag="RHN82_24430"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2943..3266)
                     /locus_tag="RHN82_RS24435"
                     /old_locus_tag="RHN82_24435"
     CDS             complement(2943..3266)
                     /locus_tag="RHN82_RS24435"
                     /old_locus_tag="RHN82_24435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_021243036.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARTGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(4273..5559)
                     /locus_tag="RHN82_RS24440"
                     /old_locus_tag="RHN82_24440"
     CDS             complement(4273..5559)
                     /locus_tag="RHN82_RS24440"
                     /old_locus_tag="RHN82_24440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002437803.1"
                     /GO_process="GO:0006935 - chemotaxis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing methyl-accepting chemotaxis
                     protein"
                     /protein_id="WP_306170312.1"
                     /translation="MFSLNLQRSAISYDAICQNKPVIEFSPEGVINKASPLFLSTMGYR
                     ADEIIGHHHRIFCPPSLVSSPEYSQFWQRLARGESFSGKYLRLAKGNRSVWLEASYIPV
                     SDRRGRVIRVIKIAADISERVHSALEQEAVVNAINRSMAIITFNPEGIVLEANENFVNA
                     TGYKRDEIIGKHHRLFCAETLYKSDEYRHFWESLNQGEFFSGLFPRLNRQGDPLWFRAT
                     YNPVFNSDGQLYKIVKFATDVTADVLRNQREQEAAVHAWDMAVQTRESAQNGANVIENS
                     ILMIDRIAQGMGAVSTDISRLNNQSESIDDMVETIRKFAMQTRLIALNAAIEAARAGAS
                     GRSFAVVAAEVRNLAASVSSATEEIEQVVASNSQLAKDVLCGIENSLMNTREGVTLMRE
                     AGEVMTSIQRNAAGVETAVKDVAISVKAG"
                     /name="PAS domain-containing methyl-accepting chemotaxis
                     protein"
     gene            complement(6473..6688)
                     /locus_tag="RHN82_RS24445"
                     /old_locus_tag="RHN82_24445"
     CDS             complement(6473..6688)
                     /locus_tag="RHN82_RS24445"
                     /old_locus_tag="RHN82_24445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021243037.1"
                     /translation="MTELEKQLLSVLEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(6695..7186)
                     /locus_tag="RHN82_RS24450"
                     /old_locus_tag="RHN82_24450"
     CDS             complement(6695..7186)
                     /locus_tag="RHN82_RS24450"
                     /old_locus_tag="RHN82_24450"
                     /inference="COORDINATES: protein motif:HMM:NF016710.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_047720453.1"
                     /translation="MSSLLTLAKDLEQQSKAQQKSTGEMLKAAFSEHEQSVRAELNASA
                     RRISDAISAHEQSMSEAMEKNRRSVLLTAGRTWLTILMVSALLIATSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQAEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            complement(<7221..7925)
                     /locus_tag="RHN82_RS24455"
                     /old_locus_tag="RHN82_24455"
                     /pseudo=""
     CDS             complement(<7221..7925)
                     /locus_tag="RHN82_RS24455"
                     /old_locus_tag="RHN82_24455"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(7864..8187)
                     /locus_tag="RHN82_RS24460"
                     /old_locus_tag="RHN82_24460"
     CDS             complement(7864..8187)
                     /locus_tag="RHN82_RS24460"
                     /old_locus_tag="RHN82_24460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_021243036.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARTGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(9199..9699)
                     /locus_tag="RHN82_RS24465"
                     /old_locus_tag="RHN82_24465"
     CDS             complement(9199..9699)
                     /locus_tag="RHN82_RS24465"
                     /old_locus_tag="RHN82_24465"
                     /inference="COORDINATES: protein motif:HMM:NF012245.3"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_process="GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="WP_310399651.1"
                     /translation="MAVQTRESAQNGANVIENSILMIDRIAQGMGAVSTDISRLNNQSE
                     SIDDMVETIRKFAMQTRLIALNAAIEAARAGASGRSFAVVAAEVRNLAASVSSATEEIE
                     QVVASNSQLAKDVLCGIENSLMNTREGVTLMREAGEVMTSIQRNAAGVETAVKDVAISV
                     KAG"
                     /name="methyl-accepting chemotaxis protein"
     Typing          complement(3380..3552)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.851"
                     /coverage="100.0"
                     /evalue="1.98e-82"
     Typing          complement(8301..8474)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="94.886"
                     /coverage="100.0"
                     /evalue="9.34e-71"
     Typing          3477..4023
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.727"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          8398..8942
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.036"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          complement(1455..2953)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.297"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(6376..7874)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.297"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tgacggttaa ggcgtggaaa cagaccagaa aaaaactcac cttgattcag actctcccag
       61 aaatgtcggt attcatcact tttgtaaagc gtctcggcac agaacagacg atgatgctta
      121 cctatgattt catcccgctt atagccagtg gcattgacga aattttcatt tgcttcaagc
      181 acgatccctt caggattgaa ggtgatgatg gccatagaac ggtttatggc atttacaact
      241 gcttcctgct caagagcaga atgcacgcgc tcagaaatat cggcagcaat tttaatgact
      301 ctgataacac ggccgcgcct gtcagaaacg gggatataac tggcttcaag ccatacggaa
      361 cgatttccct ttgccagccg caggtacttg ccactaaagc tttctcccct ggcaaggcgc
      421 tgccagaact ggctgtattc cggtgaactt accagcgagg gcgggcagaa tatacgatgg
      481 tggtgaccga ttatttcatc agccctgtac cccatagtgg agagaaaaag cgggctggct
      541 ttatttatca ccccctccgg actgaactca atgacaggct tattctgaca aattgcgtca
      601 tacgaaatgg ctgatcgctg gaggttcagg gagaacatat ttttaagtga aaacatatga
      661 atcagattgc ccggataatg taagtactgc catggttatc ggctactcct tatgtagcgt
      721 tatatattac tgcgacaggg cgggaaaaag cccgctattc agccatgccg gcatgatgtc
      781 cggctccgga tttcacgcgt aaaaaacccg ccatctttag cgggctgaac aataagaaaa
      841 tgttcatatt tacggcaaat ccgccggctc cagcagagaa gctggcgtct atcaatcttg
      901 gaagaaccag cgcagctccg catgcattaa caggcatcag aaggcaaaaa aatggctccc
      961 atccgggagc cggaaaacac tcaactgcct ggatcgcagc acatcctttg tgaaggataa
     1021 tttaagtatc ggccggattg ccgcgggcat aattttttcc gtatccgcca gacctcggct
     1081 tttaacataa caccccttgc ccgcaccgca gaaacggggt tggctgcgtt tccggtgcca
     1141 gacgcgagaa agtggcgaca gacatgacga aaaaacacca aaaacgcagc cgctggatat
     1201 tacactgatg catagtcatg cagccagatg catgcagcgt aaagtcacac taaaatcagc
     1261 gcgaacgcct taaaaaccgg caaatgcctc tccggcaact cacacagctc catcaccagg
     1321 taaaaacagt tcagcagggc ttcaataacg ttgagaattt tcatcacttc ctccgggata
     1381 agtcactacc attccggtag tcagcctatt tagcaggaac gcgatgagcc cttcttcgtg
     1441 cgggctgtca cctcatcttc ggaccatcga aactcttctg cttctgtcgg agcgtcagct
     1501 ccagctcgtt ctgacgctga tactccagtg cagccttctc ctcgatggca ttcagttccc
     1561 ggacaagcgg cgcagctgct cgcttaaact cagcacctgc gtgctcaagt ccgacacgcg
     1621 ctcgttcagc accgcgttct cccgttgcat aagaccacac atgctccgcc attccgcgaa
     1681 ggcgctctcc cattcgtcca gcctttgcga gtagtcctgc tgtagctgct ctaatacgct
     1741 cagcaactgc ttttccagct ctgtcatttt ccgctactcc tgtttcaggc ggatccatgt
     1801 cgtcccgtcg taggggatgg cttcggcttg catccccggc ggcaggataa gaaacctccg
     1861 gccgtcgctg ctctcctgat agcgcactcc ccacgtccgg gcggtcattt tcgccagggt
     1921 atcttcctgc tgacgaagat gcgtgtaatt gtcggttatc tgctgcccct gccaccacag
     1981 aatgctcccg ctcgtggcga tcagcagcgc tgacaccatc aggatggtga gccatgtcct
     2041 cccggccgtc agcaacacgc tccgccggtt tttctccatc gcctcgctca tactctgctc
     2101 gtgggcgctg atggcgtcgc tgattctcct cgcgcttgcg ttcagttccg ctctgacaga
     2161 ctgctcgtgc tcgctgaatg cggctttcag catctcgccg gtactcttct gctgcgcttt
     2221 cgattgctgc tctaagtcct tcgccagcgt taaaagactg ctcatagatg gctcccttca
     2281 gtcggatgtt tcgccccccg tcagggtcgg caatgctgat actgcttttc gttgtacgca
     2341 ccacctcaaa acctgcacct tcaagagcct cagtgacgtc ctgacggctt ttcagctccc
     2401 cggacgaggc taagctcagc agtccacggg tgatggcttc tgcggcttcc tgcttcgttt
     2461 tcggcagtcc ggccggcgtg accagcgccc ggcggttttc cggcgcgttc ggatcgtgca
     2521 gccccagtct gccgttcacg atggtctgcc aggcatcgat acgcggccgg tcggcgcggt
     2581 cgtagtacgg ctggagccgc ctgccggtca gcagctcggt attggggatc agaaagttca
     2641 gctccagacg ccccttgtcc tcgtgctcca cccacagaat gctgtactga tctttatcaa
     2701 gtccgggcat cagaacccgc tcgaagctgg ccatcagttt ttcccgctgg ccgggaggca
     2761 gatcggcctc cgcaaacgac aggacgccgg aggtgtattt cttcacgtaa ggcgacgcgt
     2821 caatcagctc ccggacttct tccggcttcc cctgcaggac gctggcgcct tcacgctgcc
     2881 ggtctttacc cagcagatag tccaccggcc cggcgccacc gccgcggcca cgtggatgaa
     2941 atttaacgat catcgtccgt tcctttctca agaacggcgt gccgcagccg ctcaagcccg
     3001 gcatcgatgg ccatcagcgc cgccacaatc tgcaccctgt catggccggt gccgccgccg
     3061 gcgttaacac ggcgggctat ctggttcagg ttattcccca tgccggcaag ctggcgcagc
     3121 agcgccggcg agattgaggg gagctttccg gtgcgcgcag gtttttcgtc caggcaggtc
     3181 tgccgcatcc acgccgccag ctgcctgccg tcacagcgct caagcagccg ccggtgctcg
     3241 tcctccgtca cccacatggt gagcatcttg ttccgtttct ctgacaaacc ggcctcctga
     3301 taacgccggg acgggcggga aagcctttcc cggtcggcgc ggggttggac aagccgcagg
     3361 cgcgtcaggc ttttggcggg tttcggggcg cagccctgaa ccagtcacgt agcgctagcg
     3421 gagtgtatac tggcttacta tattggcacg gatgcgaata tcagtgaagt gcttcatgtg
     3481 gcaggagaaa aatgcagcag cggagcgtca gcagaatatg tgatacagga tatattccgc
     3541 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg taccggctta
     3601 caggcggggc ggaaatttcc tgggagatgc cagaaagaag cttaacaggg aagcgataag
     3661 gccacggcga agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga
     3721 cgctcaaatc agtggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     3781 ggtggctccc tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg
     3841 ttatggccgc gtttgtctca ttccacgcct gacactcagt tccgggaagg cagttcgctc
     3901 caagctggac tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tatccggtaa
     3961 ctatcaactt gagtccaatc cggaaaaaca cgacaaagcg ccactggcag cagccactgg
     4021 taacaggttt agaggaggag agtcttgaag tttcgggtgc gataaggcta cactgaaagg
     4081 acagttttgg tgactgtgct cctccaagcc agttaccgcg gttcaaaaga gcgtcaactc
     4141 ttgcgaacct tcgaaaaagc caccttgccg ggtggttttt tcgttttcag agcaagagat
     4201 tacgacgcaa cggaaaggat ctcaagaaga tcatctgtga tgttcgacag atatggggat
     4261 atgcaccatg gcctatccgg ctttaactga aatggcgaca tccttaaccg cagtctcaac
     4321 ccctgcagca ttcctctgaa tgctggtcat aacttcaccc gcttcgcgca tgagtgtaac
     4381 cccttcccga gtgttcatca ggctgttttc aatgccacag agaacatcct tggccagttg
     4441 actattgctg gccacgactt gctcaatttc ttcagtcgcg ctactgacgc tcgccgcaag
     4501 attacgcact tctgcggcaa caaccgcaaa acttcttccg gatgcgccag cccttgctgc
     4561 ttcaatcgcg gcattcagtg caataagtct tgtctgcatg gcaaacttcc ggatggtttc
     4621 caccatatcg tcaatacttt cagactgatt gttgagccgt gagatatcgg tggagacagc
     4681 ccccatcccc tgcgcaattc tgtcaatcat aaggatgcta ttttcaataa cattggcacc
     4741 gttctgcgca ctttctcggg tctgcacggc catatcccat gcatgcacag cagcctcttg
     4801 ctctctctgg tttcgcagga catcagccgt aacatctgta gcgaatttca caattttata
     4861 aagctgccca tcactgttaa agacagggtt ataagttgca cggaaccaca gagggtctcc
     4921 ctgacggtta aggcgtggaa acagaccaga aaaaaactca ccttgattca gactctccca
     4981 gaaatgtcgg tattcatcac ttttgtaaag cgtctcggca cagaacagac gatgatgctt
     5041 acctatgatt tcatcccgct tatagccagt ggcattgacg aaattttcat ttgcttcaag
     5101 cacgatccct tcaggattga aggtgatgat ggccatagaa cggtttatgg catttacaac
     5161 tgcttcctgc tcaagagcag aatgcacgcg ctcagaaata tcggcagcaa ttttaatgac
     5221 tctgataaca cggccgcgcc tgtcagaaac ggggatataa ctggcttcaa gccatacgga
     5281 acgatttccc tttgccagcc gcaggtactt gccactaaag ctttctcccc tggcaaggcg
     5341 ctgccagaac tggctgtatt ccggtgaact taccagcgag ggcgggcaga atatacgatg
     5401 gtggtgaccg attatttcat cagccctgta ccccatagtg gagagaaaaa gcgggctggc
     5461 tttatttatc accccctccg gactgaactc aatgacaggc ttattctgac aaattgcgtc
     5521 atacgaaatg gctgatcgct ggaggttcag ggagaacata tttttaagtg aaaacatatg
     5581 aatcagattg cccggataat gtaagtactg ccatggttat cggctactcc ttatgtagcg
     5641 ttatatatta ctgcgacagg gcgggaaaaa gcccgctatt cagccatgcc ggcatgatgt
     5701 ccggctccgg atttcacgcg taaaaaaccc gccatcttta gcgggctgaa caataagaaa
     5761 atgttcatat ttacggcaaa tccgccggct ccagcagaga agctggcgtc tatcaatctt
     5821 ggaagaacca gcgcagctcc gcatgcatta acaggcatca gaaggcaaaa aaatggctcc
     5881 catccgggag ccggaaaaca ctcaactgcc tggatcgcag cacatccttt gtgaaggata
     5941 atttaagtat cggccggatt gccgcgggca taattttttc cgtatccgcc agacctcggc
     6001 ttttaacata acaccccttg cccgcaccgc agaaacgggg ttggctgcgt ttccggtgcc
     6061 agacgcgaga aagtggcgac agacatgacg aaaaaacacc aaaaacgcag ccgctggata
     6121 ttacactgat gcatagtcat gcagccagat gcatgcagcg taaagtcaca ctaaaatcag
     6181 cgcgaacgcc ttaaaaaccg gcaaatgcct ctccggcaac tcacacagct ccatcaccag
     6241 gtaaaaacag ttcagcaggg cttcaataac gttgagaatt ttcatcactt cctccgggat
     6301 aagtcactac cattccggta gtcagcctat ttagcaggaa cgcgatgagc ccttcttcgt
     6361 gcgggctgtc acctcatctt cggaccatcg aaactcttct gcttctgtcg gagcgtcagc
     6421 tccagctcgt tctgacgctg atactccagt gcagccttct cctcgatggc attcagttcc
     6481 cggacaagcg gcgcagctgc tcgcttaaac tcagcacctg cgtgctcaag tccgacacgc
     6541 gctcgttcag caccgcgttc tcccgttgca taagaccaca catgctccgc cattccgcga
     6601 aggcgctctc ccattcgtcc agcctttgcg agtagtcctg ctgtagctgc tctaatacgc
     6661 tcagcaactg cttttccagc tctgtcattt tccgctactc ctgtttcagg cggatccatg
     6721 tcgtcccgtc gtaggggatg gcttcggctt gcatccccgg cggcaggata agaaacctcc
     6781 ggccgtcgct gctctcctga tagcgcactc cccacgtccg ggcggtcatt ttcgccaggg
     6841 tatcttcctg ctgacgaaga tgcgtgtaat tgtcggttat ctgctgcccc tgccaccaca
     6901 gaatgctccc gctcgtggcg atcagcagcg ctgacaccat caggatggtg agccatgtcc
     6961 tcccggccgt cagcaacacg ctccgccggt ttttctccat cgcctcgctc atactctgct
     7021 cgtgggcgct gatggcgtcg ctgattctcc tcgcgcttgc gttcagttcc gctctgacag
     7081 actgctcgtg ctcgctgaat gcggctttca gcatctcgcc ggtactcttc tgctgcgctt
     7141 tcgattgctg ctctaagtcc ttcgccagcg ttaaaagact gctcatagat ggctcccttc
     7201 agtcggatgt ttcgcccccc gtcagggtcg gcaatgctga tactgctttt cgttgtacgc
     7261 accacctcaa aacctgcacc ttcaagagcc tcagtgacgt cctgacggct tttcagctcc
     7321 ccggacgagg ctaagctcag cagtccacgg gtgatggctt ctgcggcttc ctgcttcgtt
     7381 ttcggcagtc cggccggcgt gaccagcgcc cggcggtttt ccggcgcgtt cggatcgtgc
     7441 agccccagtc tgccgttcac gatggtctgc caggcatcga tacgcggccg gtcggcgcgg
     7501 tcgtagtacg gctggagccg cctgccggtc agcagctcgg tattggggat cagaaagttc
     7561 agctccagac gccccttgtc ctcgtgctcc acccacagaa tgctgtactg atctttatca
     7621 agtccgggca tcagaacccg ctcgaagctg gccatcagtt tttcccgctg gccgggaggc
     7681 agatcggcct ccgcaaacga caggacgccg gaggtgtatt tcttcacgta aggcgacgcg
     7741 tcaatcagct cccggacttc ttccggcttc ccctgcagga cgctggcgcc ttcacgctgc
     7801 cggtctttac ccagcagata gtccaccggc ccggcgccac cgccgcggcc acgtggatga
     7861 aatttaacga tcatcgtccg ttcctttctc aagaacggcg tgccgcagcc gctcaagccc
     7921 ggcatcgatg gccatcagcg ccgccacaat ctgcaccctg tcatggccgg tgccgccgcc
     7981 ggcgttaaca cggcgggcta tctggttcag gttattcccc atgccggcaa gctggcgcag
     8041 cagcgccggc gagattgagg ggagctttcc ggtgcgcgca ggtttttcgt ccaggcaggt
     8101 ctgccgcatc cacgccgcca gctgcctgcc gtcacagcgc tcaagcagcc gccggtgctc
     8161 gtcctccgtc acccacatgg tgagcatctt gttccgtttc tctgacaaac cggcctcctg
     8221 ataacgccgg gacgggcggg aaagcctttc ccggtcggcg cggggttgga caagccgcag
     8281 gcgcgtcagg cttttggcgg gtttcggggc gcagccctga accagtcacg tagcgctagc
     8341 ggagtgtata ctggcttact atattggcac ggatgcgaat atcagtgaag tgcctcatat
     8401 ggcagggaga aagaatgcag cagcgagcgt caggagaata tgtgatacag aatatattcc
     8461 gcttcctcgc tcactgactc gctccgctcg gtcgttcggc tgcggcgagc gggaccggct
     8521 tacaggcggg gcggaaattt cctgggagat gccagaaaga agcttaacga agaaagcata
     8581 aggccgcgcc aaagccgttt ttccataggc tccgcccccc tgacaagcat cacgaaatct
     8641 gacgctcaaa tcagtggtgg cgaaacccga taggactata aagataccag gcgtttcccc
     8701 ctggtggctc cctcgtggct cccccgcatt cctgcctttc ggtttaccgg tgtcactccg
     8761 ctgttatggc cgcggtttct cattccacgc ctgacactca gttccgggaa ggcagttcgc
     8821 tccaggatgg actgtatgca cgaacccccc gttcagtccg accgctgcgc cttatccggt
     8881 aactatcaac ttgagtccaa cccggaaaaa cacgactaat cgccattggc aacggccatt
     8941 ggcaaacagg atttgagagg agctagagtt cttgaagttg cggcttagtg aggctacact
     9001 gaaaggacaa ttttggtgac atgtgctccc ccaagactgt taccacggtt aagagagcgt
     9061 caatcttcct aaccttcgaa aaaccacctt gccagggtgg ttttttcgtt ttcagggcaa
     9121 gagattacga cgcggaagaa aaggatctca agaagatcat ctgtgatgtt cgacagatat
     9181 ggggatatgc accatggcct atccggcttt aactgaaatg gcgacatcct taaccgcagt
     9241 ctcaacccct gcagcattcc tctgaatgct ggtcataact tcacccgctt cgcgcatgag
     9301 tgtaacccct tcccgagtgt tcatcaggct gttttcaatg ccacagagaa catccttggc
     9361 cagttgacta ttgctggcca cgacttgctc aatttcttca gtcgcgctac tgacgctcgc
     9421 cgcaagatta cgcacttctg cggcaacaac cgcaaaactt cttccggatg cgccagccct
     9481 tgctgcttca atcgcggcat tcagtgcaat aagtcttgtc tgcatggcaa acttccggat
     9541 ggtttccacc atatcgtcaa tactttcaga ctgattgttg agccgtgaga tatcggtgga
     9601 gacagccccc atcccctgcg caattctgtc aatcataagg atgctatttt caataacatt
     9661 ggcaccgttc tgcgcacttt ctcgggtctg cacggccata tcccatgcat gcacagcagc
     9721 ctcttgctct ctctggtttc gcaggacatc agccgtaaca tctgtagcga atttcacaat
     9781 tttataaagc tgcccatcac tgttaaagac agggttataa gttgcacgga accacagagg
     9841 gtctccctga cggttaaggc gtggaaacag acc
//
