LOCUS       NZ_CP133750            10022 bp    DNA     circular CON 13-SEP-2023
DEFINITION  Klebsiella pneumoniae strain KP17 plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP133750
VERSION     NZ_CP133750.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37272496
            Assembly: GCF_031326265.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10022)
  AUTHORS   Turton,J.F. and Turton,J.A.
  TITLE     Plasmid analysis using q20 nanopore sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10022)
  AUTHORS   Turton,J.F. and Turton,J.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2023) HCAI, Fungal, AMR, AMU & Sepsis Division,
            United Kingdom Health Security Agency, 61 Colindale Avenue, London,
            London NW9 5EQ, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2023
            Assembly Method :: flye v. 2.9.1-b1780; medaka v. 1.7.2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 69x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/11/2023 16:30:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,941
            CDSs (total)                      :: 5,814
            Genes (coding)                    :: 5,640
            CDSs (with protein)               :: 5,640
            Genes (RNA)                       :: 127
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 85 of 174
            Pseudo Genes (incomplete)         :: 91 of 174
            Pseudo Genes (internal stop)      :: 25 of 174
            Pseudo Genes (multiple problems)  :: 25 of 174
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP133750.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10022
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP17"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed2"
                     /geo_loc_name="United Kingdom: England"
                     /collection_date="2022-05"
                     /genotype="ST395"
     gene            join(9548..10022,1..20)
                     /locus_tag="RG052_RS29630"
                     /old_locus_tag="RG052_29630"
     CDS             join(9548..10022,1..20)
                     /locus_tag="RG052_RS29630"
                     /old_locus_tag="RG052_29630"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_309520633.1"
                     /translation="MHNAALELFNERLPHKPYFSDDLHFGVRIAGKERAILAKYIQFNQ
                     PHAMFWLGFDVDRAGSSIDWSDRNAPAPTLTITNPENGHAHLLYALENSIRTAPDGKIK
                     PLRYAASVENALRKKLEADAGYSGLICKNPNHNHWKIAVWQPKLVHSGLAGRLSRFEHR
                     E"
                     /name="replication initiation protein"
     gene            join(9990..10022,1..432)
                     /locus_tag="RG052_RS29635"
                     /old_locus_tag="RG052_29635"
     CDS             join(9990..10022,1..432)
                     /locus_tag="RG052_RS29635"
                     /old_locus_tag="RG052_29635"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="WP_309520634.1"
                     /translation="MYTLDWLADFLDLNTGNDKEIVADYGLGRNCTLFDKTRKWAYRAI
                     RQGWPEYEQWLQACYERACAYNLQFARPLDENEVRGIAKSIAKWTSNKFSPEEFSKFVD
                     ITHSSEIQSKRGKKSGQSRRKGSLEEIKPWVAMGISRRKYFYIKKNGEIR"
                     /name="primase C-terminal domain-containing protein"
     gene            429..854
                     /locus_tag="RG052_RS29640"
                     /old_locus_tag="RG052_29640"
     CDS             429..854
                     /locus_tag="RG052_RS29640"
                     /old_locus_tag="RG052_29640"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_309520635.1"
                     /translation="MKTFVTLWTIVFLAFIAVMCTVDISNYIPSVYTVFSKNRPLVTGA
                     LSILLISVFIWLMVSLYLLKKYSVKVEKLSLGGVNILFNESATLYKKSVKNHLDSKRAI
                     FKLRKNVDAFDEVISSYYQTYQFIRDEMKILNPKKRS"
                     /name="hypothetical protein"
     gene            883..1137
                     /locus_tag="RG052_RS29645"
                     /old_locus_tag="RG052_29645"
     CDS             883..1137
                     /locus_tag="RG052_RS29645"
                     /old_locus_tag="RG052_29645"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_223703889.1"
                     /translation="MVLNRFLTNYQNNYKRWYKYISDKDEVVDRVSGDVMRFHATSINK
                     IQMQYYDYERILKGFKDVNRFFVNDVKPKFGINTDKWNW"
                     /name="hypothetical protein"
     gene            complement(1174..1788)
                     /locus_tag="RG052_RS29650"
                     /old_locus_tag="RG052_29650"
     CDS             complement(1174..1788)
                     /locus_tag="RG052_RS29650"
                     /old_locus_tag="RG052_29650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012822468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="WP_019725132.1"
                     /translation="MGKHKVFISYHHANDQYYKNALEKMNEEHEIFVNRSVSLGDIDED
                     EAPQKIREIIRDEYLRDTSVLILLVGTETKNRKHVDWELYSSMRDSTINKKSGILVVNL
                     PSTNTTYIRSTHGTNEKSEVHPTVTEWFSIDKRSTYEERFPYMPARIIDNFMKSGSYIS
                     VVNWNQIENNPETLRKLIDMTYNDREKATYDMSREMRMHNS"
                     /name="TIR domain-containing protein"
     gene            2190..2591
                     /locus_tag="RG052_RS29655"
                     /old_locus_tag="RG052_29655"
     CDS             2190..2591
                     /locus_tag="RG052_RS29655"
                     /old_locus_tag="RG052_29655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_019725133.1"
                     /translation="MTRVSISEAARLTGKSRTTLHRLIKTGELSTCSGERNTKMVDISE
                     LLRVFGPFDQPKNEHLTRQVPEQRDTAPADQSEHVIRQLKQQIEHLNILILSKDSHIDS
                     LKQAMLLLENHQAKLTEPPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(3391..3552)
                     /locus_tag="RG052_RS29660"
                     /old_locus_tag="RG052_29660"
     CDS             complement(3391..3552)
                     /locus_tag="RG052_RS29660"
                     /old_locus_tag="RG052_29660"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_162870772.1"
                     /translation="MAKHWSGQECPKTGTYGQYHDTDNSYAGSEYDRHVSKGDKFPPSK
                     NNHHFQEK"
                     /name="hypothetical protein"
     gene            3789..4034
                     /locus_tag="RG052_RS29665"
                     /old_locus_tag="RG052_29665"
     CDS             3789..4034
                     /locus_tag="RG052_RS29665"
                     /old_locus_tag="RG052_29665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012848468.1"
                     /GO_component="GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0097351 - toxin sequestering activity [Evidence IEA]"
                     /GO_process="GO:0045927 - positive regulation of growth
                     [Evidence IEA]; GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="WP_025987695.1"
                     /translation="MHAINFTDARKHFAETMKRVTDDAEPVRVMRRDAPDIVMIDAGEY
                     EALIETVYLFSNPANAEHINESMKQAERGEFVEVEY"
                     /name="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
     gene            4015..4290
                     /locus_tag="RG052_RS29670"
                     /old_locus_tag="RG052_29670"
     CDS             4015..4290
                     /locus_tag="RG052_RS29670"
                     /old_locus_tag="RG052_29670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017412855.1"
                     /GO_process="GO:0008219 - cell death [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="WP_019725129.1"
                     /translation="MSKWNIKFTRNGADDLNYWRDTDKKKFERIRQLLLSIEADPLTGI
                     GKPERLRHHKDPALYSRRIDQSHRLVYSIQNGEIIIYAARYHYGDK"
                     /name="Txe/YoeB family addiction module toxin"
     gene            4538..5443
                     /locus_tag="RG052_RS29675"
                     /old_locus_tag="RG052_29675"
     CDS             4538..5443
                     /locus_tag="RG052_RS29675"
                     /old_locus_tag="RG052_29675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_019725130.1"
                     /translation="MHNAALELFNERLPHKPYFSDDLHFGVRIAGKERAILAKYIQFNQ
                     PHAMFWLGFDVDRAGSSIDWSDRNAPAPTLTITNPENGHAHLLYALENSIRTAPDGKIK
                     PLRYAASVENALRKKLEADAGYSGLICKNPNHNHWKIAVWQPKLYTLDWLADFLDLNTG
                     NDKEIVADYGLGRNCTLFDKTRKWAYRAIRQGWPEYEQWLQACYERACAYNLQFARPLD
                     ENEVRGIAKSIAKWTSNKFSPEEFSKFVDITHSSEIQSKRGKKSGQSRRKGSLEEIKPW
                     VAMGISRRKYFYIKKNGEIR"
                     /name="replication initiation protein"
     gene            5440..6147
                     /locus_tag="RG052_RS29680"
                     /old_locus_tag="RG052_29680"
     CDS             5440..6147
                     /locus_tag="RG052_RS29680"
                     /old_locus_tag="RG052_29680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004198042.1"
                     /translation="MKTFVTLWTIVFLAFIAVMCTVDISNYIPSVYTVFSKNRPLVTGA
                     LSILLISVFIWLMVSLYLLKKYSVKVEKLSLGGVNILFNESATLYKKSVKNHLDSKRAI
                     FKLRKNVDAFDEVISSYYQTYQFIRDEMKILNPKKDREIYDFSNEMLMVLNRFLTNYQN
                     NYKRWYKYISDKDEVVDRVSGDVMRFHATSINKIQMQYYDYERILKGFKDVNRFFVNDV
                     KPKFGINTDKWNW"
                     /name="hypothetical protein"
     gene            complement(6184..6798)
                     /locus_tag="RG052_RS29685"
                     /old_locus_tag="RG052_29685"
     CDS             complement(6184..6798)
                     /locus_tag="RG052_RS29685"
                     /old_locus_tag="RG052_29685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012822468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="WP_019725132.1"
                     /translation="MGKHKVFISYHHANDQYYKNALEKMNEEHEIFVNRSVSLGDIDED
                     EAPQKIREIIRDEYLRDTSVLILLVGTETKNRKHVDWELYSSMRDSTINKKSGILVVNL
                     PSTNTTYIRSTHGTNEKSEVHPTVTEWFSIDKRSTYEERFPYMPARIIDNFMKSGSYIS
                     VVNWNQIENNPETLRKLIDMTYNDREKATYDMSREMRMHNS"
                     /name="TIR domain-containing protein"
     gene            7200..7601
                     /locus_tag="RG052_RS29690"
                     /old_locus_tag="RG052_29690"
     CDS             7200..7601
                     /locus_tag="RG052_RS29690"
                     /old_locus_tag="RG052_29690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_019725133.1"
                     /translation="MTRVSISEAARLTGKSRTTLHRLIKTGELSTCSGERNTKMVDISE
                     LLRVFGPFDQPKNEHLTRQVPEQRDTAPADQSEHVIRQLKQQIEHLNILILSKDSHIDS
                     LKQAMLLLENHQAKLTEPPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(8401..8562)
                     /locus_tag="RG052_RS29695"
                     /old_locus_tag="RG052_29695"
     CDS             complement(8401..8562)
                     /locus_tag="RG052_RS29695"
                     /old_locus_tag="RG052_29695"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_162870772.1"
                     /translation="MAKHWSGQECPKTGTYGQYHDTDNSYAGSEYDRHVSKGDKFPPSK
                     NNHHFQEK"
                     /name="hypothetical protein"
     gene            8799..9044
                     /locus_tag="RG052_RS29700"
                     /old_locus_tag="RG052_29700"
     CDS             8799..9044
                     /locus_tag="RG052_RS29700"
                     /old_locus_tag="RG052_29700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012848468.1"
                     /GO_component="GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0097351 - toxin sequestering activity [Evidence IEA]"
                     /GO_process="GO:0045927 - positive regulation of growth
                     [Evidence IEA]; GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="WP_025987695.1"
                     /translation="MHAINFTDARKHFAETMKRVTDDAEPVRVMRRDAPDIVMIDAGEY
                     EALIETVYLFSNPANAEHINESMKQAERGEFVEVEY"
                     /name="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
     gene            9025..9300
                     /locus_tag="RG052_RS29705"
                     /old_locus_tag="RG052_29705"
     CDS             9025..9300
                     /locus_tag="RG052_RS29705"
                     /old_locus_tag="RG052_29705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017412855.1"
                     /GO_process="GO:0008219 - cell death [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="WP_019725129.1"
                     /translation="MSKWNIKFTRNGADDLNYWRDTDKKKFERIRQLLLSIEADPLTGI
                     GKPERLRHHKDPALYSRRIDQSHRLVYSIQNGEIIIYAARYHYGDK"
                     /name="Txe/YoeB family addiction module toxin"
ORIGIN
        1 gatttgaaca cagggaatga caaggaaatc gttgccgact acggcttagg ccgtaactgc
       61 acgttgttcg acaaaacccg taagtgggca tatcgcgcca tccgtcaggg ctggcctgag
      121 tatgagcagt ggctgcaagc ctgctacgaa cgcgcttgcg catacaacct gcaatttgca
      181 cggcctctgg atgaaaatga ggtaagaggg atagctaaga gcattgctaa atggacatca
      241 aataaatttt cacctgaaga attcagtaaa tttgttgata ttactcattc ctctgaaatt
      301 caatcaaaga gagggaaaaa gtcaggacag tcaaggagga aaggaagcct agaggaaata
      361 aaaccttggg tagctatggg aataagcagg cggaagtatt tttatatcaa gaaaaatggt
      421 gaaataagat gaaaaccttc gtaactcttt ggactatagt ttttttagca tttattgccg
      481 tgatgtgtac tgttgatata tctaattaca ttccgtctgt ttacacagtt tttagtaaga
      541 atagacctct tgtaactggc gccctttcta ttcttttgat atctgttttt atttggctga
      601 tggtatccct ctacttactt aaaaagtatt cggttaaggt tgagaaactt agccttggtg
      661 gagtgaatat acttttcaat gagagtgcta cactttacaa aaagagtgta aaaaatcacc
      721 tagattcaaa aagagcaatt ttcaaattaa gaaaaaacgt tgatgccttt gatgaggtta
      781 tttcatcata ttatcagact taccaattta ttcgtgatga aatgaaaata ctaaacccta
      841 aaaaaagatc gtgagattta tgatttcagc aatgagatgc tcatggtgtt aaatagattt
      901 ctaactaact accagaacaa ctacaagcgt tggtataagt acatatcaga caaagatgag
      961 gttgttgata gggttagcgg tgatgtcatg agatttcatg caaccagcat aaataaaata
     1021 caaatgcagt attatgatta tgagcgtatt ttaaaaggtt ttaaagatgt taaccggttc
     1081 tttgttaatg atgtgaagcc taagtttgga atcaatacag ataagtggaa ctggtaaatt
     1141 aaaggagtag ccaaggctac tccttggata gtactatgaa ttatgcattc tcatttctct
     1201 cgacatatca tacgttgctt tttctctatc gttataagtc atgtctatta attttctgag
     1261 tgtttctggg ttgttttcta tttgattcca gttgaccact gatatataag acccgctctt
     1321 catgaagttg tctattattc ttgctggcat atatgggaac ctttcctcat aagttgagcg
     1381 cttatcaata gaaaaccatt cagttacggt aggatgaact tctgattttt cattagttcc
     1441 atgtgttgaa cgaatgtacg tagtatttgt tgacgggaga ttgacgacta aaattcctga
     1501 ttttttgtta atggtactat ctctcatgct agagtaaagt tcccaatcta cgtgttttct
     1561 gttttttgtt tcagttccaa caagaaggat aagaactgat gtatctctaa gatattcatc
     1621 tcttattatc tctcttattt tttgtggggc ttcatcttca tctatatcac ctaatgatac
     1681 tgagcgattc acgaatattt catgttcctc attcattttt tcaagtgcgt ttttgtagta
     1741 ctgatcattc gcatggtggt agctaatgaa taccttatgc tttcccattt ttattgtcct
     1801 ttttttgttt gtgtgcactc tattttgcac ttgaacctat atcagataac agctctcttt
     1861 tagagagctc tttttttctc agccagcaca catagggtcc cttcagcaag gtctcgccat
     1921 gactggttta gggcgggctc gttgcttccc taaacgcctt gagaagagag ctatcacctc
     1981 actttattct tacgtaccgc tcgccgcagt cttatgaccg aacgtagtga gcgaatgcgc
     2041 gaggaagcgg aaagtcattt gttgcctgca ttttgcacgg tcactcctgt gtttcatcgc
     2101 ccttctgaaa aacacatcac ttctggacca gtatgtcgta agctggcccc tgtttccttg
     2161 agactgaatg tattaaagga tgataactga tgaccagagt gtctattagt gaggctgcac
     2221 ggctgacggg taagagccgg acaacgctgc acaggttaat caaaacaggt gaactgtcca
     2281 cttgtagcgg tgaacgaaat acaaaaatgg tcgatatatc agagctttta cgtgttttcg
     2341 gaccgtttga ccaaccgaag aatgaacacc ttactcgaca agtacctgaa cagcgtgata
     2401 cagcgcctgc tgaccagagt gaacatgtca ttcgtcaact gaaacagcag attgaacacc
     2461 ttaacatatt gattttatcg aaggattcgc acattgacag cctgaaacag gccatgctcc
     2521 ttcttgaaaa tcaccaggca aaattaactg aacccccggc gccgtggtgg aaattctgga
     2581 aaaaatcctg accccgtcat gcttcagttt tcctctccgg ccattccggg gagttcctga
     2641 accttcccga aaatcgtcag atctctgaac gctcctcacc acgaaaaata cccacacccc
     2701 acgcaaaatt ttaattttgc agatttgtat ttaatatcag cagcttactt tactgaaatt
     2761 tcgtttttta aattttattt atatagatcc gactctatga cgagtcgtca tagagtcccc
     2821 ggcgagtcgt catagagtcg gtaacgactc aggcctataa aaacaatgag ttaataacga
     2881 cccgactcta gatccgactc cccgtttggt tgttttttag acgtggcgac tctgggtacg
     2941 actctagtga agagtcgccg gggaatcgtg tggtgagtcg tccggcgact tgccataacg
     3001 tgattgccgg ccgtggacga accgattcct gaacgtgctg aaaaccggtt ttccggctgc
     3061 gccggcaacg cttcacggcc acgcaataaa ttgcgtcccc gttcaccgtt ttcggatggg
     3121 aagaacgccg gaaacaggac ttcccctggc tgaatggtgt caatttccgc gctatgcact
     3181 tgtgcgcata ctcatgcatg ccgtaaaaac agagcctgcg cgtttctggc gggttttcgg
     3241 gtggtttgtt gccttgttta ccggtttccc gtcagaaacg ccctgagggc atctcaggcg
     3301 gtgctgataa cggatgttat ggtaaatcaa gtgaatggca ggtacaaaaa aagccccaga
     3361 gggcttttag tttagacttc tgaagatatt ttatttttct tggaagtggt ggttgttttt
     3421 gctcggaggg aacttatcac ccttgctcac atggcgatca tattcagaac cagcgtatga
     3481 gttgtcggta tcatgatatt gaccgtaagt acctgttttt ggacattctt gcccagacca
     3541 atgttttgcc atttactttt cctttgctcg ttttaggagc taagctccta gcggtacgaa
     3601 attgtgccgc acttcataaa tgtaattgct tgaaattatg cttcaatgta ttgacttgtt
     3661 gtttttgtgg ttattcgatt gagttcacat tttgagctga tttggttgtg ggtttttagg
     3721 gaaatatctt tatgtactta tttgcgtaca tgttctatgc taagccccaa caaatggggg
     3781 ttaacaacat gcatgcaatc aattttactg atgcccgtaa acattttgcc gagacgatga
     3841 agcgcgtcac tgatgatgct gaaccggtac gtgtcatgcg ccgtgatgcg cctgatattg
     3901 ttatgatcga tgcgggagag tatgaggcgc taatcgaaac ggtctatctg ttcagcaatc
     3961 cggcgaacgc agagcatatc aatgagtcaa tgaaacaggc ggagcgcggc gaatttgtcg
     4021 aagtggaata ttaagttcac taggaatggc gctgatgacc ttaactactg gcgtgatact
     4081 gacaaaaaga agtttgagcg tatccggcaa ctgttgctca gcatcgaggc tgatccgctg
     4141 acaggtatag gaaaacccga acggctgcgg caccacaaag atccggcact ctactcccgc
     4201 aggattgatc agagccaccg tctggtctat tcaatccaga acggagaaat catcatctac
     4261 gcagcccgtt atcactatgg tgacaaatag caaaagtcga tatagcccgc ttgatgcggg
     4321 cttttcatgc aaggcgtaca acggatgtta gggtaagtcg ctgtcagatc gacacaaagg
     4381 caaagtcggt gtaacgttag cctcagaaaa aacaaagccc cggaaatcat gtcatatctt
     4441 ggcggatcgc atgataacca gggctaggtc gaaaccttga aaggatatta gcacatgaac
     4501 cgtgccagac aacagcccgc cataagcgca gggcacattg cataacgccg cactcgaact
     4561 tttcaacgaa cgcttaccac acaagccgta cttttcggac gatctgcact ttggtgtccg
     4621 tatcgccggt aaggaacgcg ctattctggc gaaatatatc cagttcaatc agcctcacgc
     4681 catgttctgg ctgggatttg acgttgaccg cgccggttcc tccatcgact ggagcgaccg
     4741 caacgcgcct gcaccgacgc tgaccataac taaccccgaa aacggacacg cgcacctgct
     4801 gtacgcgctg gaaaactcga tacgcaccgc gccggacggc aaaataaaac cgttacgcta
     4861 cgccgcctcc gtggagaatg cgctacgtaa aaaattagag gctgacgctg gatattcggg
     4921 gctcatctgc aaaaatccga accacaacca ctggaaaatc gccgtgtggc agccgaagct
     4981 ctacactctg gactggctgg ccgactttct cgatctgaac acagggaatg acaaggaaat
     5041 cgttgccgac tacggcttag gccgtaactg cacgttgttc gacaaaaccc gtaagtgggc
     5101 atatcgcgcc atccgtcagg gctggcctga gtatgagcag tggctgcaag cctgctacga
     5161 acgcgcttgc gcatacaacc tgcaatttgc acggcctctg gatgaaaatg aggtaagagg
     5221 gatagctaag agcattgcta aatggacatc aaataaattt tcacctgaag aattcagtaa
     5281 atttgttgat attactcatt cctctgaaat tcaatcaaag agagggaaaa agtcaggaca
     5341 gtcaaggagg aaaggaagcc tagaggaaat aaaaccttgg gtagctatgg gaataagcag
     5401 gcggaagtat ttttatatca agaaaaatgg tgaaataaga tgaaaacctt cgtaactctt
     5461 tggactatag tttttttagc atttattgcc gtgatgtgta ctgttgatat atctaattac
     5521 attccgtctg tttacacagt ttttagtaag aatagacctc ttgtaactgg cgccctttct
     5581 attcttttga tatctgtttt tatttggctg atggtatccc tctacttact taaaaagtat
     5641 tcggttaagg ttgagaaact tagccttggt ggagtgaata tacttttcaa tgagagtgct
     5701 acactttaca aaaagagtgt aaaaaatcac ctagattcaa aaagagcaat tttcaaatta
     5761 agaaaaaacg ttgatgcctt tgatgaggtt atttcatcat attatcagac ttaccaattt
     5821 attcgtgatg aaatgaaaat actaaaccct aaaaaagatc gtgagattta tgatttcagc
     5881 aatgagatgc tcatggtgtt aaatagattt ctaactaact accagaacaa ctacaagcgt
     5941 tggtataagt acatatcaga caaagatgag gttgttgata gggttagcgg tgatgtcatg
     6001 agatttcatg caaccagcat aaataaaata caaatgcagt attatgatta tgagcgtatt
     6061 ttaaaaggtt ttaaagatgt taaccggttc tttgttaatg atgtgaagcc taagtttgga
     6121 atcaatacag ataagtggaa ctggtaaatt aaaggagtag ccaaggctac tccttggata
     6181 gtactatgaa ttatgcattc tcatttctct cgacatatca tacgttgctt tttctctatc
     6241 gttataagtc atgtctatta attttctgag tgtttctggg ttgttttcta tttgattcca
     6301 gttgaccact gatatataag acccgctctt catgaagttg tctattattc ttgctggcat
     6361 atatgggaac ctttcctcat aagttgagcg cttatcaata gaaaaccatt cagttacggt
     6421 aggatgaact tctgattttt cattagttcc atgtgttgaa cgaatgtacg tagtatttgt
     6481 tgacgggaga ttgacgacta aaattcctga ttttttgtta atggtactat ctctcatgct
     6541 agagtaaagt tcccaatcta cgtgttttct gttttttgtt tcagttccaa caagaaggat
     6601 aagaactgat gtatctctaa gatattcatc tcttattatc tctcttattt tttgtggggc
     6661 ttcatcttca tctatatcac ctaatgatac tgagcgattc acgaatattt catgttcctc
     6721 attcattttt tcaagtgcgt ttttgtagta ctgatcattc gcatggtggt agctaatgaa
     6781 taccttatgc tttcccattt ttattgtcct ttttttgttt gtgtgcactc tattttgcac
     6841 ttgaacctat atcagataac agctctcttt tagagagctc tttttttctc agccagcaca
     6901 catagggtcc cttcagcaag gtctcgccat gactggttta gggcgggctc gttgcttccc
     6961 taaacgcctt gagaagagag ctatcacctc actttattct tacgtaccgc tcgccgcagt
     7021 cttatgaccg aacgtagtga gcgaatgcgc gaggaagcgg aaagtcattt gttgcctgca
     7081 ttttgcacgg tcactcctgt gtttcatcgc ccttctgaaa aacacatcac ttctggacca
     7141 gtatgtcgta agctggcccc tgtttccttg agactgaatg tattaaagga tgataactga
     7201 tgaccagagt gtctattagt gaggctgcac ggctgacggg taagagccgg acaacgctgc
     7261 acaggttaat caaaacaggt gaactgtcca cttgtagcgg tgaacgaaat acaaaaatgg
     7321 tcgatatatc agagctttta cgtgttttcg gaccgtttga ccaaccgaag aatgaacacc
     7381 ttactcgaca agtacctgaa cagcgtgata cagcgcctgc tgaccagagt gaacatgtca
     7441 ttcgtcaact gaaacagcag attgaacacc ttaacatatt gattttatcg aaggattcgc
     7501 acattgacag cctgaaacag gccatgctcc ttcttgaaaa tcaccaggca aaattaactg
     7561 aacccccggc gccgtggtgg aaattctgga aaaaatcctg accccgtcat gcttcagttt
     7621 tcctctccgg ccattccggg gagttcctga accttcccga aaatcgtcag atctctgaac
     7681 gctcctcacc acgaaaaata cccacacccc acgcaaaatt ttaattttgc agatttgtat
     7741 ttaatatcag cagcttactt tactgaaatt tcgtttttta aattttattt atatagatcc
     7801 gactctatga cgagtcgtca tagagtcccc ggcgagtcgt catagagtcg gtaacgactc
     7861 aggcctataa aaacaatgag ttaataacga cccgactcta gatccgactc cccgtttggt
     7921 tgttttttag acgtggcgac tctgggtacg actctagtga agagtcgccg gggaatcgtg
     7981 tggtgagtcg tccggcgact tgccataacg tgattgccgg ccgtggacga accgattcct
     8041 gaacgtgctg aaaaccggtt ttccggctgc gccggcaacg cttcacggcc acgcaataaa
     8101 ttgcgtcccc gttcaccgtt ttcggatggg aagaacgccg gaaacaggac ttcccctggc
     8161 tgaatggtgt caatttccgc gctatgcact tgtgcgcata ctcatgcatg ccgtaaaaac
     8221 agagcctgcg cgtttctggc gggttttcgg gtggtttgtt gccttgttta ccggtttccc
     8281 gtcagaaacg ccctgagggc atctcaggcg gtgctgataa cggatgttat ggtaaatcaa
     8341 gtgaatggca ggtacaaaaa aagccccaga gggcttttag tttagacttc tgaagatatt
     8401 ttatttttct tggaagtggt ggttgttttt gctcggaggg aacttatcac ccttgctcac
     8461 atggcgatca tattcagaac cagcgtatga gttgtcggta tcatgatatt gaccgtaagt
     8521 acctgttttt ggacattctt gcccagacca atgttttgcc atttactttt cctttgctcg
     8581 ttttaggagc taagctccta gcggtacgaa attgtgccgc acttcataaa tgtaattgct
     8641 tgaaattatg cttcaatgta ttgacttgtt gtttttgtgg ttattcgatt gagttcacat
     8701 tttgagctga tttggttgtg ggtttttagg gaaatatctt tatgtactta tttgcgtaca
     8761 tgttctatgc taagccccaa caaatggggg ttaacaacat gcatgcaatc aattttactg
     8821 atgcccgtaa acattttgcc gagacgatga agcgcgtcac tgatgatgct gaaccggtac
     8881 gtgtcatgcg ccgtgatgcg cctgatattg ttatgatcga tgcgggagag tatgaggcgc
     8941 taatcgaaac ggtctatctg ttcagcaatc cggcgaacgc agagcatatc aatgagtcaa
     9001 tgaaacaggc ggagcgcggc gaatttgtcg aagtggaata ttaagttcac taggaatggc
     9061 gctgatgacc ttaactactg gcgtgatact gacaaaaaga agtttgagcg tatccggcaa
     9121 ctgttgctca gcatcgaggc tgatccgctg acaggtatag gaaaacccga acggctgcgg
     9181 caccacaaag atccggcact ctactcccgc aggattgatc agagccaccg tctggtctat
     9241 tcaatccaga acggagaaat catcatctac gcagcccgtt atcactatgg tgacaaatag
     9301 caaaagtcga tatagcccgc ttgatgcggg cttttcatgc aaggcgtaca acggatgtta
     9361 gggtaagtcg ctgtcagatc gacacaaagg caaagtcggt gtaacgttag cctcagaaaa
     9421 aacaaagccc cggaaatcat gtcatatctt ggcggatcgc atgataacca gggctaggtc
     9481 gaaaccttga aaggatatta gcacatgaac cgtgccagac aacagcccgc cataagcgca
     9541 gggcacattg cataacgccg cactcgaact tttcaacgaa cgcttaccac acaagccgta
     9601 cttttcggac gatctgcact ttggtgtccg tatcgccggt aaggaacgcg ctattctggc
     9661 gaaatatatc cagttcaatc agcctcacgc catgttctgg ctgggatttg acgttgaccg
     9721 cgccggttcc tccatcgact ggagcgaccg caacgcgcct gcaccgacgc tgaccataac
     9781 taaccccgaa aacggacacg cgcacctgct gtacgcgctg gaaaactcga tacgcaccgc
     9841 gccggacggc aaaataaaac cgttacgcta cgccgcctcc gtggagaatg cgctacgtaa
     9901 aaaattagag gctgacgctg gatattcggg gctcatctgc aaaaatccga accacaacca
     9961 ctggaaaatc gccgtgtggc agccgaagct tgtacactct ggactggctg gccgactttc
    10021 tc
//
