LOCUS       NZ_CP133711             2968 bp    DNA     circular CON 25-APR-2024
DEFINITION  Acinetobacter baumannii strain CRAb2 plasmid unnamed05, complete
            sequence.
ACCESSION   NZ_CP133711
VERSION     NZ_CP133711.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37223190
            Assembly: GCF_038441325.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Talsma,D.T., Pimenta de Oliveira Monteiro,R., Flores-Vallejo,R.D.C.,
            Heuvelmans,M., Le,T.N., Hendrickx,A.P.A., Rosema,S., van Dijl,J.M.
            and Bathoorn,M.
  TITLE     Nosocomial transmission of tet(x3), blaNDM-1, and blaOXA-97
            -carrying Acinetobacter baumannii conferring resistance to
            eravacycline and omadacycline
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2968)
  AUTHORS   Talsma,D.T., Pimenta de Oliveira Monteiro,R., Flores-Vallejo,R.D.C.,
            Heuvelmans,M., Le,T.N., Hendrickx,A.P.A., Rosema,S., van Dijl,J.M.
            and Bathoorn,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-SEP-2023) Medical Microbiology and Infection
            Prevention, University Medical Center Groningen, Hanzeplein 1,
            Groningen 9700RB, Netherlands
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: UniCycler v. 0.4.4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 20x
            Sequencing Technology :: Oxford Nanopore MinION and Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_038441325.1-RS_2024_04_24
            Annotation Date                   :: 04/24/2024 16:07:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,226
            CDSs (total)                      :: 4,130
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 96
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 26 of 108
            Pseudo Genes (incomplete)         :: 70 of 108
            Pseudo Genes (internal stop)      :: 26 of 108
            Pseudo Genes (multiple problems)  :: 11 of 108
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP133711.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="CRAb2"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:470"
                     /plasmid="unnamed05"
                     /geo_loc_name="Netherlands"
                     /lat_lon="51.883331 N 5.433331 E"
                     /collection_date="2021-05-11"
                     /collected_by="Thuy-Nga Le"
     gene            complement(join(2681..2968,1..639))
                     /gene="repM"
                     /locus_tag="RGU80_RS21120"
                     /old_locus_tag="RGU80_21155"
     CDS             complement(join(2681..2968,1..639))
                     /gene="repM"
                     /locus_tag="RGU80_RS21120"
                     /old_locus_tag="RGU80_21155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005140758.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_121774293.1"
                     /translation="MKNGLVVKDNALMNASYNLEVTEQRLILLAIISARETGQGITSDS
                     KLEIHASDYAIQFSVAKETAYEALKSAVNNLFERQFSFREETKKGTGIVRSRWVSRIKY
                     IDDAAILEITFAPDVVPLITRLEEHFTSYQIKQIAQLTSKYAIRLYELLIAWRTTGKVP
                     ELELSEFRNRLGIASNEYTAMNNFKSRVLEPSIKQINEHTDITVTYEQHKKGRTITGFS
                     FRFKQKQQAKKIETNRDPNTPDFFIKMTDAQRHLFANKMSEMPEMGKYSQGTESYQQFA
                     IRIADMLLQPEKFRELYPILEKAGFKE"
                     /name="repM"
     gene            complement(1212..2345)
                     /gene="mobV"
                     /locus_tag="RGU80_RS21125"
                     /old_locus_tag="RGU80_21160"
     CDS             complement(1212..2345)
                     /gene="mobV"
                     /locus_tag="RGU80_RS21125"
                     /old_locus_tag="RGU80_21160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005080191.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_121774291.1"
                     /translation="MSFAILRIQKLKSFADVGGSLSHNYRNRETLNADDARTHLNEHTL
                     DTNEKCMSAIRDRIPEKRRKDAVLCIEHLVTASPEWDGWGTEKETAFFEQSRKWLESKY
                     GKKNVVSTTIHRDETTPHLVAYVVPVDEETGRLNAKKFIGGSRHTLSQMQTDFAVEVKD
                     LGLDRGVQGSKAKHTSIQEYYEKLNNYENEPGIEKGLTYEVPEPEFFESKNVYGERVAE
                     AVAAQIIDQIAPRFDNANLLASQTKKLKKELLNTRKTLDEVQKRAKPYLDIINEYNHPN
                     LEKEFNKQVAKLKDNFDSALEHHRFLKRQEEQERFNQQRELRNQLHLEQEQKKQLVEQE
                     RQEKERLALLRRQELENQRKNEPKKPDNGNNNDYSPS"
                     /name="mobV"
     gene            complement(2496..2684)
                     /locus_tag="RGU80_RS21130"
                     /old_locus_tag="RGU80_21165"
     CDS             complement(2496..2684)
                     /locus_tag="RGU80_RS21130"
                     /old_locus_tag="RGU80_21165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010591710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_121774292.1"
                     /translation="MIEKEITKFEKEILLQDKISQLENELKEFSDLQKKAYSERLQKSI
                     VGLENRIYRIKKMLYTT"
                     /name="hypothetical protein"
     Typing          complement(1216..2372)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="87.047"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 tttatgctgt tcatacgtca cagtaatgtc tgtgtgttca ttgatctgct taatagacgg
       61 ctctaataca cggcttttaa agttgttcat tgctgtgtat tcattactag ctatgcctaa
      121 tctatttcta aattctgata gttcaagctc agggactttg cctgtagttc gccaagcaat
      181 aagaagttca tataaacgga tagcgtactt acttgtaagc tgtgctattt gcttgatttg
      241 ataacttgtg aagtgttctt caagtctagt gattaatggt acaacgtcag gcgcaaaagt
      301 gatttcaagt attgctgcgt catcaatgta tttaattctg ctaacccatc gtgaccgtac
      361 aatgccagtg cctttttttg tttcttctct aaatgaaaat tgacgctcaa ataaattatt
      421 tacagcactt tttagtgcct cataagctgt ttcttttgca acactgaact gaattgcata
      481 gtcactagca tgtatttcta atttgctatc tgacgtaatc ccttgccctg tttctcttgc
      541 actaatgatt gctagaagta ttaagcgctg ttctgttact tctaaattat agctggcatt
      601 cattaacgca ttatctttca ctactaaccc atttttcata ttagttattt catacaatca
      661 tgtattaaaa gatacattat atgactatgt accttaatgc aaggaatctg tatctttata
      721 gcaaggaatc tgtatcttta tagcaaggaa tctgtatctt tatagcaagg aatctgtatc
      781 tttataagct ctgaaacata tgtatagcaa tgctttcagg ctatctaaaa gcatttaaaa
      841 gcatttaaaa acattaaaag cccaaaaaag gcaaaatcaa aggctatggc actcgctttg
      901 ctcgtgtcgt ttcaagagca aaatcaaagt caaaagcata gataatgcat tcgctatgct
      961 catgcttttt taagagcaaa agcatcaata cttcgctcgt agacactcgc taacaagtct
     1021 ctccagaagc aaaaaagagc aaaagcagtt tttcagaggg ggaataggcg aattttcatt
     1081 gtgtcgctcg tagacactcg ctaaatgctc atcatttgcg ttctaagagc atttttttat
     1141 ttcaattata cttttatgca taaaaggcca aacagggcta aaacccccgg ttttttgcgt
     1201 ttaaacgggt attatgaggg tgagtagtcg ttattattgc cattatcagg ttttttaggc
     1261 tcatttttcc gctgattttc taattcttga cgtcttaaaa gtgctaaacg ttctttttct
     1321 tgcctttctt gctcaaccag ttgttttttt tgctcttgct ctaagtgtaa ttgattgcga
     1381 agttcacgtt gttggttaaa tctttcttgt tcttcctgtc tttttaggaa tctatgatgc
     1441 tcaagtgctg aatcaaaatt atcttttaat ttagcaactt gcttattaaa ttctttctca
     1501 agatttggat gattatattc gttgattata tccaaatagg gttttgctcg tttctgtact
     1561 tcatcaagcg tttttctagt gtttaacagt tcttttttta atttttttgt ttgactagcc
     1621 aataaattag cattgtcgaa tcgaggtgca atttgatcaa ttatctgagc agccacagct
     1681 tctgcgactc tctcaccgta aacattttta gattcaaaaa actcaggttc aggcacttca
     1741 taggtgagtc ctttttcaat acctggttca ttctcataat tgttcaattt ttcataatat
     1801 tcttgaatag acgtatgttt tgctttgctc ccctgtaccc cgcgatctaa tcctaaatcc
     1861 tttacttcaa ctgcaaaatc agtctgcatc tgtgaaagtg tatgtcgaga tccaccaata
     1921 aattttttag catttaaacg tcccgtttct tcgtcaacgg gaacaacata cgcaactaaa
     1981 tgtggagttg tttcatctct atgaatcgtt gtactgacca catttttttt accatatttg
     2041 ctttcaagcc atttccttga ctgttcaaaa aatgcagttt ctttttcagt tccccagccg
     2101 tcccattctg gtgatgcagt cacaagatgt tcaatacaca aaaccgcatc ttttcttcgc
     2161 ttctcaggaa ttcggtccct gattgcggac atacattttt cgtttgtatc tagcgtatgt
     2221 tcattcaaat gagtacgagc atcatctgca tttaacgttt ctcgattgcg ataattatgc
     2281 gaaagactac cgccaacgtc tgcaaacgat ttcaattttt gaatacgcaa aatagcaaat
     2341 gacatttgat aaatccgata ccgaaaaaac acacgatagc agaggttttt gagctttgcg
     2401 atagcgaagt ggactcactc cactttcaaa gcacctaaag gtacattgaa agttcatgag
     2461 taaggggaat cctcccctta acccctgaca acctttcagg ttgtataaag cattttcttg
     2521 attcgataga ttctattttc taaacccacg atactttttt gaagccgttc gctatatgct
     2581 tttttttgaa gatcagaaaa ttcttttagt tcattttcga gctgagagat tttgtcttgt
     2641 agcaaaatct ctttctcaaa ttttgttatc tctttttcaa tcattctttg aatccggctt
     2701 tttctaaaat tggataaagc tctctgaatt tttcaggttg taaaagcatg tcggcgatgc
     2761 gaatagcaaa ttgttggtag ctttctgtac cttgtgagta tttccccatt tcgggcattt
     2821 cagacatttt gttagcgaat aaatgccgtt gagcatcggt cattttgata aaaaagtcag
     2881 gtgtgtttgg atctctgtta gtttctattt tttttgcttg ttgcttctgc ttaaatctga
     2941 atgaaaagcc tgtaattgtc cgcccttt
//
