LOCUS       NZ_CP133709            10891 bp    DNA     circular CON 25-APR-2024
DEFINITION  Acinetobacter baumannii strain CRAb2 plasmid unnamed03, complete
            sequence.
ACCESSION   NZ_CP133709
VERSION     NZ_CP133709.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37223190
            Assembly: GCF_038441325.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 10891)
  AUTHORS   Talsma,D.T., Pimenta de Oliveira Monteiro,R., Flores-Vallejo,R.D.C.,
            Heuvelmans,M., Le,T.N., Hendrickx,A.P.A., Rosema,S., van Dijl,J.M.
            and Bathoorn,M.
  TITLE     Nosocomial transmission of tet(x3), blaNDM-1, and blaOXA-97
            -carrying Acinetobacter baumannii conferring resistance to
            eravacycline and omadacycline
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10891)
  AUTHORS   Talsma,D.T., Pimenta de Oliveira Monteiro,R., Flores-Vallejo,R.D.C.,
            Heuvelmans,M., Le,T.N., Hendrickx,A.P.A., Rosema,S., van Dijl,J.M.
            and Bathoorn,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-SEP-2023) Medical Microbiology and Infection
            Prevention, University Medical Center Groningen, Hanzeplein 1,
            Groningen 9700RB, Netherlands
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: UniCycler v. 0.4.4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 20x
            Sequencing Technology :: Oxford Nanopore MinION and Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_038441325.1-RS_2024_04_24
            Annotation Date                   :: 04/24/2024 16:07:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,226
            CDSs (total)                      :: 4,130
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 96
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 26 of 108
            Pseudo Genes (incomplete)         :: 70 of 108
            Pseudo Genes (internal stop)      :: 26 of 108
            Pseudo Genes (multiple problems)  :: 11 of 108
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP133709.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10891
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="CRAb2"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:470"
                     /plasmid="unnamed03"
                     /geo_loc_name="Netherlands"
                     /lat_lon="51.883331 N 5.433331 E"
                     /collection_date="2021-05-11"
                     /collected_by="Thuy-Nga Le"
     gene            join(10202..10891,1..12)
                     /locus_tag="RGU80_RS21000"
                     /old_locus_tag="RGU80_21035"
     CDS             join(10202..10891,1..12)
                     /locus_tag="RGU80_RS21000"
                     /old_locus_tag="RGU80_21035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002058850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="WP_002058850.1"
                     /translation="MRHSYHIEKHYIERSGWLRAAVLGANDGIISVTSLVVGIAASGAS
                     SQTLLVTCVAGLISGAASMAAGEYISVKSQQDIEKNDLKMEARELKLHPEHELQELKNI
                     YIQRGLEPTLAEDVAKQLTMHNSLDAHARDEIGISVHTSAKPFLAASSSALAFSVGSLF
                     PLISMMLLPEQYLDKGVMLIGVLTLGIMGALASYAGGVSIWRGSVRVMIWGIVAMLFSA
                     WIGSLFNVAVT"
                     /name="VIT family protein"
     gene            complement(360..890)
                     /locus_tag="RGU80_RS21005"
                     /old_locus_tag="RGU80_21040"
     CDS             complement(360..890)
                     /locus_tag="RGU80_RS21005"
                     /old_locus_tag="RGU80_21040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017397202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_005065050.1"
                     /translation="MKSLTVLELSKLYNINRQTIYNNIKKGILSKNSQNKIDLAEAIRV
                     FGEPVKKQDVKEPVKIDSPNSAEVLLLRQQIDMLKNQLDDAKDRESFYQNQIETMQRLL
                     EAPKPNMTTFTDQELVQPSIAKPDPEPDTAEAKHDGLTTPERRENKWIPVPEHIEPEPE
                     KRGFWSRFFRPYD"
                     /name="plasmid replication DNA-binding protein"
     gene            complement(969..1898)
                     /gene="repM"
                     /locus_tag="RGU80_RS21010"
                     /old_locus_tag="RGU80_21045"
     CDS             complement(969..1898)
                     /gene="repM"
                     /locus_tag="RGU80_RS21010"
                     /old_locus_tag="RGU80_21045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065049.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_005065049.1"
                     /translation="MSELIVKDNALIQASYTLDTVEQRLILLAIAEARETGHGITENSL
                     LEVHASSYINTFNVEKHTAYTVLRDASKSLFDRYVTYHDINPKTGKDRSFHCRWVDKIG
                     YEPQSGIVFLRFTQDIVPLITRLEENFTKYELEQVSRLTSSYAIRLYELLIQWRSAGKT
                     PIFDLSIFRQQLGVEAHQYKTMSNFKTYVLDFALKQVNELTDITAKYEQHKKGRSISGF
                     SFSFKQKKTNSDKVIKGTDTLALFTKMSDKQRHLFANKLSELPEMGQYSEGTESFQQFA
                     IRIAEMLQDYQKFQELFPYLQKVGFAPA"
                     /name="repM"
     gene            complement(2596..3309)
                     /locus_tag="RGU80_RS21015"
                     /old_locus_tag="RGU80_21050"
     CDS             complement(2596..3309)
                     /locus_tag="RGU80_RS21015"
                     /old_locus_tag="RGU80_21050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006581718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005065071.1"
                     /translation="MAGRKTFALTQQEIIDLDKHIRALPFKRRMQDAFLAMISERESMK
                     QLNEEDLKLIQRCRYERNTYNKRIVQLQLIQKTEPQKLKKLELNILELSKKTDIDAYFA
                     MHDALNEVLKIKRNQTSEKNITAKVERVLNPEKYNQAPLSKSLRKQQDHIKYFIGSLYL
                     NLLEQSGLERSNSNEDLDRMLDLFKQAQSGAEKNNAFSLDQNRKTDILSAKSLYEMEKL
                     IKQAKEDPRNPFNKA"
                     /name="hypothetical protein"
     gene            3765..4649
                     /locus_tag="RGU80_RS21020"
                     /old_locus_tag="RGU80_21055"
     CDS             3765..4649
                     /locus_tag="RGU80_RS21020"
                     /old_locus_tag="RGU80_21055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_005065070.1"
                     /translation="MFYLNLKHNIKKEQSSLNAFHYLTRTAHFENQKDNEKLEFMRYGN
                     MPKWAENKPKLFWKSADQFEISRGRTSSTLTIALPKELILEQRAELVQKLIDQFAGQYQ
                     FPYTAVIHNHPSEITGEDQPHLHLMYSERTISDDIERPPEQFFKQYRPKNPTQGGAQKL
                     TADALGFGRNQIKVFREKTQELMNLFLEKYAPTKKVMVYGVEIEVKNQVSCLSNDDFNQ
                     KFGTKLQDVPQIPRWKLYSADPIIQLDVEAQKNTIKKIRNQNNAELYGKEYDLEISRRH
                     VLTQKKDPGFSLD"
                     /name="MobA/MobL family protein"
     gene            5015..5842
                     /locus_tag="RGU80_RS21025"
                     /old_locus_tag="RGU80_21060"
     CDS             5015..5842
                     /locus_tag="RGU80_RS21025"
                     /old_locus_tag="RGU80_21060"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_342039595.1"
                     /translation="MEFFFILVGMILLVPVCIALSQSNILRDEFDEDDSPKTKNTATKF
                     QLQEEERKKALEYSKMMDEKRKRVAEEKRKRVAEEKRKRVAEEEPKREEEKLKIKEEER
                     KREEEIKYTKEKKLEEFSIYKNQLKEKILIIEKKDNTIVINGIPVSSWLDLTFYQLAYL
                     IKDNVKKYDDATLRAEYDKYNPNTYGGLRLLRESGVLIIEIGNKEYPDCILLNRNIQLV
                     GYENTPLKEILKTIKESEKSSKAMLEEQERQEQEQEVLHRQRTQNGYYNEYDM"
                     /name="hypothetical protein"
     gene            6207..6494
                     /locus_tag="RGU80_RS21030"
                     /old_locus_tag="RGU80_21065"
     CDS             6207..6494
                     /locus_tag="RGU80_RS21030"
                     /old_locus_tag="RGU80_21065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_002124950.1"
                     /translation="MELYFEWDEAKNQKNQKKHDVSFETASLVFEDPLRISIQDRHTNG
                     EERWQTIGMVKGVLMLLVAHTIIDDDDCEIIRIISARQVTKAERNQYEHG"
                     /name="BrnT family toxin"
     gene            6481..6792
                     /locus_tag="RGU80_RS21035"
                     /old_locus_tag="RGU80_21070"
     CDS             6481..6792
                     /locus_tag="RGU80_RS21035"
                     /old_locus_tag="RGU80_21070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_005065064.1"
                     /translation="MSMVRYTRKELNEKFSDKQDAEIKRLLAKGTVPDEQLDLSDIPEI
                     TDWSHAVRHGQFYRPVKQQTSVRLDADVLAWLKAQGKGYQTRMNKILREAMLEDLKNH"
                     /name="BrnA antitoxin family protein"
     gene            7149..7364
                     /locus_tag="RGU80_RS21040"
                     /old_locus_tag="RGU80_21075"
     CDS             7149..7364
                     /locus_tag="RGU80_RS21040"
                     /old_locus_tag="RGU80_21075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004693381.1"
                     /GO_component="GO:0005829 - cytosol [Evidence IEA]"
                     /GO_function="GO:0003676 - nucleic acid binding [Evidence
                     IEA]; GO:0003723 - RNA binding [Evidence IEA]; GO:0001072 -
                     transcription antitermination factor activity, RNA binding
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="WP_000071892.1"
                     /translation="MSNSNVVKGTVKWFNETKGFGFIQQESGPDVFAHFSEIASSGFKT
                     LFEGQQVEFSVAQGQKGPNAVNIVAI"
                     /name="cold-shock protein"
     gene            7462..7689
                     /locus_tag="RGU80_RS21045"
                     /old_locus_tag="RGU80_21080"
     CDS             7462..7689
                     /locus_tag="RGU80_RS21045"
                     /old_locus_tag="RGU80_21080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005166620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000921865.1"
                     /translation="MLKVNDLIAKSKNGTEIIVSLIPLHKMQNTRQGLKQIEVGKRVLL
                     ESGIEVDLNLDGRTFYTSLNQLFKLNHKVA"
                     /name="hypothetical protein"
     gene            7756..7953
                     /locus_tag="RGU80_RS21050"
                     /old_locus_tag="RGU80_21085"
     CDS             7756..7953
                     /locus_tag="RGU80_RS21050"
                     /old_locus_tag="RGU80_21085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000476222.1"
                     /translation="MFKQTLSVKDQFGVKHAIQATVDHEFANTQSHLNIKHITVDGEDI
                     RPSFEMLFQSTLSGKIFKII"
                     /name="hypothetical protein"
     gene            8140..8460
                     /locus_tag="RGU80_RS21055"
                     /old_locus_tag="RGU80_21090"
     CDS             8140..8460
                     /locus_tag="RGU80_RS21055"
                     /old_locus_tag="RGU80_21090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000897309.1"
                     /translation="MLFIETSIFTKQIKELVSDEEYRQLQQDLLVQPDKGDLIKNGGGI
                     RKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAYPKSVKDNLTDKETSILRQLVKEQF
                     HG"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            8453..8725
                     /gene="nadS"
                     /locus_tag="RGU80_RS21060"
                     /old_locus_tag="RGU80_21095"
     CDS             8453..8725
                     /gene="nadS"
                     /locus_tag="RGU80_RS21060"
                     /old_locus_tag="RGU80_21095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="WP_000369780.1"
                     /translation="MDNNLFDDLVASIKEAGAIKRKEIKASRVTELELPDIKEVREKTG
                     LTQAEFAARLHISARTLQNWEQGRRYPTGPAATLIRILDAHPTLI"
                     /name="nadS"
     gene            complement(9237..9854)
                     /locus_tag="RGU80_RS21065"
                     /old_locus_tag="RGU80_21100"
     CDS             complement(9237..9854)
                     /locus_tag="RGU80_RS21065"
                     /old_locus_tag="RGU80_21100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="WP_002054947.1"
                     /translation="MAQNTATSSTDLLIRFLHIVIILSFTGAYLTGDAEEWHQVHMAFG
                     YTLGISLILRILWQFLASRFAHTQPSGASRRFNVIKPFVQRYWAQPQQWLSPAFLKAAS
                     SSLFQLSILGAFCLMPLTVLAGFLTDYTHSHSLKEVHELFANLFLATVLLHLTALTLNS
                     VLLKKWLAKRMFWGTQSHSWFTLFASVLSLGVLVAFWFWYFS"
                     /name="cytochrome b/b6 domain-containing protein"
     Typing          complement(983..1898)
                     /name="rep_cluster_1226"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="85.884"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          3766..4646
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gctgtgacct aatatacttt gtgtatctga tcacaatatt ttaatccaat aaattattat
       61 aggttttgag tagttattat tcatgagttc tcctaacttt tagatactta tattacatat
      121 gatctaacct gattagaggt caaaactaag atactcaatc atctgcttca aagattttta
      181 aaattaacat aatacacctt atacgaaatg tgttataatt ttccttagaa atcaacgctg
      241 tagtttcttt tttgcccaac ctccccaagg tgggcttttt ttttatgatt tttcacgttc
      301 cacgcttatt atttaatcaa agtttcacga ctttattcat attaattaaa caatcgtggt
      361 taatcatagg gtctaaaaaa acggctccaa aagcctcttt tctcaggctc tggttcaatg
      421 tgctctggca ctggaatcca cttattttcc ctccgctctg gagtagtcaa tccgtcatgt
      481 ttagcttctg ctgtgtcagg ttcgggatct ggtttggcta tgctgggttg cactaattct
      541 tgatcggtaa acgtagtcat attaggtttt ggggcttcta ataggcgttg catcgtttca
      601 atttggttct gatagaaaga ttcccgatct ttggcatcat caagctggtt tttcagcatg
      661 tctatctgct gtctaagtag taaaacttct gccgaatttg gactgtctat ttttacaggt
      721 tcttttacat cctgtttttt gacaggttcc ccgaaaacac ggatagcctc ggctagatcg
      781 atcttattct gggaattttt acttaaaatt cctttcttta tgttgttgta aatcgtttgt
      841 ctattgatat tgtaaagctt agaaagctcg agaactgtaa ggcttttcat gttgtaaatc
      901 tatgcctaaa cctgtcaaaa gttgttgtct attagactgt ctaataaaca aatgatcaag
      961 tttaaatttt aggcaggtgc aaagccaact ttttgaagat aagggaataa ttcttggaat
     1021 ttttgataat cttggagcat ttctgcaatg cgtatagcaa attgttggaa gctttcggtg
     1081 ccttctgaat attgccccat ctcaggtaat tcagaaagtt tgttggcaaa gagatgacgt
     1141 tgtttatcac tcattttggt gaaaagggct aatgtatccg ttcccttaat tactttgtca
     1201 gagttggttt ttttctgctt aaaactgaaa gaaaaaccgg aaattgaacg tcctttttta
     1261 tgttgttcat atttagccgt tatatcggtt aactcattta cttgcttgag ggcaaaatct
     1321 aaaacatacg ttttaaaatt actcattgtt ttgtattgat gagcttcgac accaagttgt
     1381 tgtctaaaga tcgatagatc aaaaattggc gttttaccag cagatctcca ttgaattaat
     1441 aactcgtata accgaatagc ataagagcta gttaatctcg aaacctgctc caattcatat
     1501 ttagtaaagt tttcttccag tcgagttatg agtggaacaa tgtcttgcgt gaaacgtagg
     1561 aaaacgatcc cggattgagg ttcatatcca attttatcga cccaacgaca gtgaaagcta
     1621 cgatccttac cagtcttagg attaatgtca tgatacgtga catagcgatc aaacaagctt
     1681 ttagatgcat cccgaagcac ggtataagca gtgtgtttct cgacattaaa ggtattgata
     1741 taactacttg catgtacttc taaaaggcta ttttcagtta tcccgtgccc tgtttcccga
     1801 gcttcagcaa tggctaatag gatcagcctt tgttcaactg tatctagggt atagctagct
     1861 tgaattaaag cattatcctt tacgattaat tcactcattt ctaataataa atttataatt
     1921 aaactaggtt gtacaataga ctattttaat tgaattgtaa atcgacttaa tttggggtcg
     1981 ataaacctag ttttaaggtc gataaaccta gttttatggg tcgataaacc tagttttatg
     2041 ggtcgataaa cctagtttta tgggtcgata aacctagttt tattaactct aaaactatta
     2101 tgaataaagg gttacagctt acttaaaagc atttaaaaga ttaaaaataa ttaaaagccc
     2161 aggtaaagaa aaattgccct tggttttcgc ttcgctcaaa ctctattgaa cttcgctttc
     2221 gctcagttcg tcggggcaat tttttggtga gcacttacgt gactttaaaa aagcaaaagc
     2281 aactcggaat tcgctacgct cataaaactt ttttactcgc tacgctcgat ctagactcaa
     2341 atttctacaa cttttccatg cttatttagg tttcacagcc taaaaagcgg tttttcaggg
     2401 agtctaggca aaattttcca aggatcgctc gtagacactc gctttgttct tatcggtaaa
     2461 atcaaatgat ctccaaaata ctctaatttg cgtttagagc actctaattt ttttattgat
     2521 ggggaatata ggtttaggct aaaaaaggca tatacggctt caacagtgta tttaaaccct
     2581 attatttgag tcaatctaag ctttattgaa aggattcctt ggatcttctt tggcttgctt
     2641 aatcaatttc tccatttcat atagcgattt agccgataaa atatctgttt ttctattttg
     2701 atccaggcta aatgcattgt tcttttctgc tccggattgt gcctgcttaa ataaatcgag
     2761 cattcgatct aaatcttcgt ttgaatttga tcgctctaaa ccgctttgtt ccaataagtt
     2821 taaatatagt gatccgataa aatatttaat gtggtcttgc tgttttctta aagatttact
     2881 aaggggggcc tggttatatt tctcagggtt taaaactcgt tcgaccttcg ctgtaatatt
     2941 cttttcagaa gtctgatttc ttttaatttt taaaacttca tttaatgcat catgcattgc
     3001 aaagtaagca tcaatatctg tttttttact taattctaga atattcaatt ctaatttttt
     3061 taatttctgt ggctcggttt tttggatcag ttgtaattga actatacgtt tattataagt
     3121 attgcgctca taacgacatc gttgaataag ttttaaatct tcttcattca gttgtttcat
     3181 tgattctcgt tcagaaatca ttgctaaaaa agcatcttgc attcgtcttt taaacggcag
     3241 cgctcgaata tgtttatcca aatcgatgat ttcttgttgt gttaaggcaa atgtttttct
     3301 accggccaaa gcggatttcc tgattttggg tatctaaatt gatgttagcg gaattctgat
     3361 ggtgttgaaa caatatttta tgttatacat aaaatattgt ttatctcgtt tccgtgtctt
     3421 tttttaacaa tgattgcttt agcaatcatt gttaaaaaaa gacacttcta ctcgctcctg
     3481 cggaggctac ataaaccact gatttaatta ttaaattatg agtttttctt cgggttttaa
     3541 cggtattttt cttaatggtg aaaaagtaag caatgtctgc cgttacaaga aatgggaaag
     3601 gggtgcaaca aggaaaacag cgaattttag acgagtgcaa caaacttttt tgatgaaata
     3661 tcgatgtttg caacaagaag agactgctct ctgcaacaag aattagcccc ttgttacaag
     3721 aaaatttgct gtttcgttac acgtaatttg tttgaggtga actgatgttt tatttaaatc
     3781 taaagcataa tattaaaaaa gagcagagtt ctctgaatgc attccattat ttaactcgga
     3841 cggcacattt tgaaaatcaa aaagacaatg aaaaattaga gtttatgcgg tatggaaata
     3901 tgcccaaatg ggcagaaaat aaaccaaaac ttttttggaa aagtgctgat caatttgaaa
     3961 tttcccgggg aagaacaagc tctactttaa ccattgcttt accaaaagag ttaatactag
     4021 aacaacgagc agaattggtt cagaagttaa tagatcagtt tgccggacaa tatcaatttc
     4081 cttataccgc agtcattcat aatcatccca gtgaaattac tggagaggat caaccacatt
     4141 tacatttgat gtattcagaa agaacaattt cagatgatat tgaacgacca ccagagcagt
     4201 tttttaaaca gtaccggcca aaaaatccaa ctcaaggtgg tgcgcaaaag ttaacagctg
     4261 atgcattagg ttttgggcga aatcaaatca aagtattccg tgaaaaaact caagaattaa
     4321 tgaatttgtt tttagaaaaa tatgcaccaa ctaaaaaagt aatggtttat ggtgtagaaa
     4381 ttgaagtgaa aaatcaggtc agttgcctat ctaatgacga tttcaatcaa aaattcggga
     4441 ccaaattaca ggatgtacca caaatccctc gttggaaact ttatagcgct gatccaatta
     4501 ttcagcttga tgttgaagct caaaaaaaca caattaaaaa aattagaaat cagaacaatg
     4561 ctgaacttta tggcaaggaa tatgatttag aaatatctag acgccatgtg cttactcaga
     4621 aaaaagatcc tggctttagc ttagattgat ttttataagt ggaaaatttg tgttttttga
     4681 acatggaatc ttgaaaaaac ttatccatat tgtgagtgat ttttagccac aacatggaaa
     4741 atttgtgttt tttaggctgc aatgatggat agattgtgtt tttgattcag gtcaataagg
     4801 aaaacctaga tttttgccca taaatgtaaa atctaggttt ttcgcataag gtgtattatg
     4861 ttaattttag gaaatcttta aaatttctaa aaatttagta gttactcaat tcttataatt
     4921 taaaataaat tattcaaata gtgctcatct tattatttaa tcttttttgt ttaataacta
     4981 tacttaacca atatttttac atgatattga aagtatggaa ttttttttta ttttggttgg
     5041 tatgatattg cttgtacctg tatgtatagc tctatctcag tcaaatatat tgagagatga
     5101 atttgatgaa gatgactctc ctaaaacaaa gaatacagct actaaatttc aactccagga
     5161 agaagaacgt aaaaaagcat tagaatattc aaaaatgatg gatgaaaaac gtaagcgagt
     5221 agctgaagaa aaacgtaagc gagtagctga agaaaaacgt aagcgagtag ctgaagaaga
     5281 acctaaacgt gaagaagaaa aacttaagat taaggaagaa gagcgaaaac gcgaagaaga
     5341 aataaaatat acgaaagaaa aaaagcttga agaatttagt atttataaga atcagctcaa
     5401 agaaaagatc cttattattg aaaaaaaaga caataccatt gtaatcaatg gaatacctgt
     5461 aagctcttgg ttagatttaa cattttatca attagcttac ttaataaaag ataatgtaaa
     5521 aaaatatgat gatgctacat taagagcaga atatgacaaa tacaatccca atacatatgg
     5581 tggattacgt ctcttaagag aatctggtgt gttgattatt gagattggaa ataaagaata
     5641 tccggactgt atattactca atagaaatat ccaacttgtt ggttatgaga atactcctct
     5701 aaaagaaata ttaaaaacta taaaagaatc tgaaaaaagc agtaaagcta tgcttgagga
     5761 acaggaacgt caagaacaag aacaagaagt tttacataga caaagaactc aaaatggata
     5821 ttacaatgaa tatgatatgt aacgtagaat ccgattagaa agctagagcg aattagtaat
     5881 tcaatcccat atgtcttaat agatcgggtg tttaatataa acttctaatt tgcaatctca
     5941 gaaactagaa ttttccacca atcccctatc catttaacat aatggccgtt atccgaaatc
     6001 gtcttttata agttaaataa aatcatatgc ttaactattt tacatcaccc gtaaccaccg
     6061 caaaaatccg ctttggaaac aaagcggatt ttttttgagc aagtttgata cttcttgcca
     6121 ataattttga ctaaaaatta ctccaattag cttgagtgat acaaatttgt gatacattat
     6181 tgtatataca aatttgaatg ttttttatgg aactgtattt cgaatgggat gaggctaaaa
     6241 accaaaagaa tcagaaaaaa catgatgtat cttttgaaac ggcaagcctc gtttttgaag
     6301 atcctttacg gatctcaatc caggatcgac ataccaatgg tgaagaacgt tggcaaacga
     6361 tcggtatggt taaaggtgta ctgatgttat tggtggctca tacaatcatt gacgatgatg
     6421 actgtgagat catacgaatc attagtgcaa ggcaagtcac taaagcggaa cgcaatcaat
     6481 atgagcatgg ttagatatac acgcaaagaa ctgaatgaaa aattcagtga caagcaagat
     6541 gctgaaatta aacgcttgct tgcaaaagga actgtaccag atgaacaatt agatctatct
     6601 gacattcctg agattaccga ctggagtcat gctgttcgcc atgggcaatt ttatcgtcca
     6661 gtgaagcaac aaacatctgt tcgtctagat gcagatgtac ttgcatggtt aaaggctcaa
     6721 ggcaaaggct atcagacacg catgaataaa attctgcgtg aagcaatgct agaagatctt
     6781 aagaatcatt gatgaaaatg ggagctatag actcccattt ttgcttcgca taagagattt
     6841 tatgttaaag aaaatattaa ataatataat aaataactta ttgaatttaa ttaattttta
     6901 aagaaaaaaa tatggatttt ataagaaaaa ttaatattta tatcaaaaaa cttgattatt
     6961 tttattttta ctgtatattt aacttaacta gaaaaaaagt cttgtttcgg taaatcttcc
     7021 ttaagtatcg cagttttagt cataatcctt cgttttttag attttaagtc tcattcttta
     7081 ttattttcaa agttattaat cagtcaataa aatgactaaa aaattattaa aaattagtag
     7141 gatatattat gtcaaactca aatgttgtta aaggtactgt taagtggttt aacgaaacta
     7201 aaggttttgg tttcattcaa caagaatcag gtccagacgt ttttgctcat ttcagcgaaa
     7261 tcgcgagttc aggtttcaag accttatttg aaggccagca agttgaattc agtgtagctc
     7321 aaggtcaaaa aggaccgaat gctgtaaata ttgttgctat ctaagtaata ataagcagta
     7381 ataaaaagca ctcataagtg ctttttttaa gctttaaaat gttttcaaaa ttgaaaaaat
     7441 ctctaaaaaa taggatataa gttgttaaaa gtaaatgatt taattgcaaa atctaaaaat
     7501 ggtactgaaa ttattgtttc attaattcct ctacataaaa tgcaaaatac acgtcaaggt
     7561 ttaaaacaaa tcgaagtagg taaaagagtt ctactggaat ctggaatcga agttgattta
     7621 aatttggatg gccgtacatt ttacacttct ctaaatcagc tatttaaatt aaatcataaa
     7681 gttgcttagc gataaatttt taaacatcaa taatttatgt gggatttcca ctgcaatttt
     7741 tttaaggaaa tatatatgtt taaacagact ctcagcgtaa aagaccagtt tggcgttaaa
     7801 cacgctattc aagccacagt ggatcatgag tttgccaaca cacaatctca tttgaatata
     7861 aaacatatca ccgttgatgg tgaagatatt cgacctagtt ttgaaatgct ttttcaaagt
     7921 accttaagtg gaaaaatatt taaaatcatc tagaaatata gtttttataa aatctatagt
     7981 ctgaatgaaa tatcaggacc tactcgctat catgcgggtt ttttatacct aatatttata
     8041 tttgtaagcc tttgcttatg cgaaacgaga gttcacagct ctcgtttttt tattctaaaa
     8101 ctgctaaaat tcttatacgc cattgacgta cttaaaccta tgttattcat tgaaaccagt
     8161 atttttacta agcaaatcaa agagcttgtg agtgatgaag agtatcgtca gctccagcaa
     8221 gatctcttgg tacagcctga taagggcgac ttaatcaaga atggtggtgg aattcgtaaa
     8281 gtacgttgtg ctcagggcaa taaaggcaaa agtggcggta tccgtgtgat ctattattgg
     8341 gttacagaag atgatcagat ctttttcctg gtggcttatc caaagtctgt gaaagataat
     8401 ctgacagaca aagaaacctc gattctacgt caattagtaa aggagcaatt tcatggataa
     8461 caacttattt gatgacctgg ttgcttcaat caaagaagct ggtgccatca agcgtaagga
     8521 gataaaggca agtcgagtca cagagcttga attaccggat attaaagaag tacgtgagaa
     8581 aacaggctta acccaggctg aatttgctgc acgtttgcat atcagcgcga gaaccctgca
     8641 aaactgggaa caaggccgtc gctatccgac tggtcctgca gcgactttaa ttcgtatttt
     8701 agatgctcat ccaactttaa tttaagaagt ttagataata aaaaatcccg acgtcatgtc
     8761 gggatttttc ggtatggagc gcataggtag ggctgactac ctgatggtca tggtgcctga
     8821 ctgattatta ttgttgtttt aagtcattga acatcctgtc catatagatc gatcataaga
     8881 gcttcatcgg gggatagata cagacaattt cgtgaaacgc tgtaagccaa aacgtacaaa
     8941 aaataatcaa aatttatccc aatggttatc cgcagaaatt gtggattctt ttgggtaaaa
     9001 aaggcttaaa aactctatgg aattgctatt tttgatcatt atttaaccaa ttttattggc
     9061 aagaagtatc aaacttgctc aaaaaaaatc cgctttggaa acaaagcgga tttttgcggt
     9121 agtttcgggt tatgtaaaat agctaagtac atgattttat ttaacttata aaagacgatt
     9181 tcggataagg tgtattatgt taaatagagc ttcatctgtg ttgctctcat aaaagcttag
     9241 ctaaaatacc aaaaccagaa agcgaccaac acgcctagtg aaaggactga agcaaacagg
     9301 gtgaaccatg aatgtgattg tgtcccccaa aacatacgct ttgctagcca ttttttaagt
     9361 agaacactat tcagtgtcag tgctgtcaag tgtagtaata cagtagctaa aaacagattt
     9421 gcgaatagtt catgcacttc ttttaaactg tggctatgcg tatagtctgt taaaaagcct
     9481 gccagtacag ttaagggcat caggcaaaaa gcacccagta tgctcagctg aaacaggctg
     9541 gaactggctg cttttaaaaa tgctggggaa agccattgct gcggttgcgc ccaatagcgc
     9601 tgcacaaatg gcttgatcac attaaatcga cggctcgccc ctgaaggctg agtatgagca
     9661 aatcgagaag caagaaactg ccataaaatt cttaatatca ggctaatacc aagtgtatag
     9721 ccaaacgcca tatgcacttg atgccattct tctgcatccc ccgtgagata agccccagta
     9781 aagctcaaaa taatgacgat atgcagaaat cgaatcagta aatctgtaga agatgttgct
     9841 gtgttctgag ccataaaaaa cctttgttga ctgtatttta gttgcagaat agcggctcaa
     9901 gctgaggtga atattaaaga atacagtcaa aaaaaaatgg cgctcattag agcgcaattt
     9961 taaaattgga agatcatgtg aatattaatt ttgtctacaa ttagcttaat acggattata
    10021 cgaagcctga acattggagt cggttttttt aatacgtata acacccatta ttaaacaagg
    10081 ttattttaaa tgtaatggat atgcatcagt agtgacttat attctctatt gtctagctca
    10141 attaaggttt acagataaaa tatttactat aatcattctg ataacagcaa gggactatat
    10201 tgtgcgccat tcttatcata tcgaaaaaca ttatattgaa cggtcagggt ggctacgtgc
    10261 ggctgtacta ggggctaatg atgggattat ttctgtaaca agcctagtag tcggaatagc
    10321 agccagtggt gcttcttctc aaacattatt agtaacatgt gttgcgggat taatttctgg
    10381 ggcggcttca atggctgcag gtgaatatat ttccgttaaa tcacaacaag atattgaaaa
    10441 aaatgatctg aaaatggagg cacgtgagtt aaaactccat cctgaacatg aactgcagga
    10501 actaaaaaat atttatatac aacgtggttt agaaccaact ttagctgaag atgtcgctaa
    10561 acaactaact atgcataatt ctttagatgc acatgctcgt gatgaaattg gcatttcagt
    10621 tcatacctct gccaagccat ttctagcggc atcttcttca gccctcgcat tttctgtagg
    10681 ttcattattt cctttaattt caatgatgct tctgcctgaa caatatttag ataaaggtgt
    10741 catgctgatt ggagttttaa ctctcggaat tatgggagca ttggcgagtt atgctggcgg
    10801 tgtaagtatc tggagaggtt ctgttcgggt tatgatttgg ggaattgtcg caatgttatt
    10861 cagtgcttgg atcggatcac tatttaatgt t
//
