LOCUS       NZ_CP133670             5594 bp    DNA     circular CON 13-SEP-2023
DEFINITION  Acinetobacter bereziniae strain UCO-553 plasmid p5kb_UCO-553,
            complete sequence.
ACCESSION   NZ_CP133670
VERSION     NZ_CP133670.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN34266403
            Assembly: GCF_031326185.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter bereziniae
  ORGANISM  Acinetobacter bereziniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 5594)
  AUTHORS   Opazo-Capurro,A.F., Xanthopoulou,K., Gonzalez-Munoz,P., Jerez,C.,
            Aguilera,F., Matus,M., Amsteins,L., Higgins,P.G. and
            Gonzalez-Rocha,G.
  TITLE     Characterisation of plasmids harbouring the tigecycline-resistance
            gene tet(Y) among carbapenem-resistant Acinetobacter bereziniae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5594)
  AUTHORS   Opazo-Capurro,A.F., Xanthopoulou,K., Gonzalez-Munoz,P., Jerez,C.,
            Aguilera,F., Matus,M., Amsteins,L., Higgins,P.G. and
            Gonzalez-Rocha,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2023) Microbiologia, Universidad de Concepcion,
            Barrio Universitario S/N, Concepcion, Bio-Bio 4070386, Chile
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/11/2023 16:11:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,329
            CDSs (total)                      :: 4,226
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 103
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 25 of 66
            Pseudo Genes (incomplete)         :: 46 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 16 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP133670.1.
            Bacteria and source DNA available from Laboratorio de Investigacion
            en Agentes Antibacterianos (LIAA).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5594
                     /organism="Acinetobacter bereziniae"
                     /mol_type="genomic DNA"
                     /strain="UCO-553"
                     /isolation_source="tracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:106648"
                     /plasmid="p5kb_UCO-553"
                     /geo_loc_name="Chile: Santiago"
                     /lat_lon="33.26 S 70.38 W"
                     /collection_date="2021-02-16"
                     /collected_by="Hospital de Urgencia Asistencia Pblica"
     gene            join(4279..5594,1..499)
                     /locus_tag="RG306_RS21635"
                     /old_locus_tag="RG306_21635"
     CDS             join(4279..5594,1..499)
                     /locus_tag="RG306_RS21635"
                     /old_locus_tag="RG306_21635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004706791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_004706791.1"
                     /translation="MIVDFFRHGSGLSKGCLDYLLGEDREREHAQVLSGDVDLTAQLID
                     SSPFAKKYTSGCLSFYEHALSDQDKQQIMQNFEECLFPGLDKDQYQILWVQHQDKVNQD
                     TGETRLELNFVIPNVELSTGKRLQPFYAPVDLDRVDLFKQITNAEHQLYDPDDPNNQQL
                     LINKKNLPKDVKDFKDQLHQRIYQAVVDGKVADRQKLVEWLESNEIKVTRQTQKSISIE
                     NPYEGATRPIRLSGEVYEQGFSATGEYRQEVQQRIATYRGTTSERYRANVTDYQRQLEH
                     KSQYHSDRYPAVRREISAEPTEQLTADREAARPVPSLATVQIEPFKANERADTDKRTAS
                     SEQKPTETAYHFEYGNDFGSCYFNYSHYCARFYGQKQVQRDTDTQYDHQRETNASGSPE
                     PIGGQFDVQHMQTAKQQSPTSMYSDQQGRRGLAEQLHGADGVLSDDRIRNTVIENHRRT
                     TAAITATTNSVAAATAEFRRDAKENYPSLISTIKGNGERTDRLSANTKVIIDNTDTISR
                     IGKKYSDLYRADQWQSQGDEPNYNELRGRTGENLNHTTVSLVTSSQSSAEFARNVRTIE
                     ANIVQMKERKKEMEQPQPKRDRGMNFGM"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(732..2687)
                     /locus_tag="RG306_RS21640"
                     /old_locus_tag="RG306_21640"
     CDS             complement(732..2687)
                     /locus_tag="RG306_RS21640"
                     /old_locus_tag="RG306_21640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017389689.1"
                     /GO_function="GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase dimerization domain-containing
                     protein"
                     /protein_id="WP_004870594.1"
                     /translation="MKVKFLTVSLMFILPFSVFAANGQVEKAVIESNNAYKTKYNLDDM
                     TEFNYAQKGFIAKPTGQIKSEKGNVIWDFDAFNFLNDPAPNTVNPALWRQAKLNNNVGL
                     FKVTDRVWQIRGFDLANMTIIQGDTGWIIVDTLTSKETAEAALKFARQHLGEQKISAII
                     FTHSHIDHFGGALGVLSTEEINARKTPIIAPQGFMEEATSENLMAGSAMIRRATYMYGT
                     FLPKNAEGLVDTGLGKAVAVGKMGILEPTQLITQAEQKMTIDGLDFVFYNVPGSEAPAE
                     LTFSIPSLKLYNGAEILSHTMHNLYTLRGAKVRDALKWVGYLDQAMQHAKASDVLIAQH
                     HWPVWGNDNIQDFIKTQRDVYKFTHDQTVRYMNSGFNGAEIAEKIQLPAALDQKLYAHG
                     YYGTLKHNVKAIYQYYMGWFDAHPSNLDPLPPKAVAKKYIELAGGENNALKNARDAYAQ
                     ADYRWAAEILKHIVLNNPQNQQAKDLLANTYRQLGYAAEASTWRNFFLVGAQELQNNVP
                     LQNTSDPSDLLIHTPTERFLEAMATNLDVENLKNENQCINLVLSDTQENFSLWVENSIM
                     QFKRHDDSKDLASDCPTLTVTKPLYLKMITGQIKGVKVLLSNESKVKGNPLEIGKFFAM
                     FKRPDSTFPIVTRPND"
                     /name="alkyl sulfatase dimerization domain-containing
                     protein"
     gene            3888..4289
                     /locus_tag="RG306_RS21645"
                     /old_locus_tag="RG306_21645"
     CDS             3888..4289
                     /locus_tag="RG306_RS21645"
                     /old_locus_tag="RG306_21645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004706792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_004706792.1"
                     /translation="MEIDSVTKQKVVRERTFKVRLSESEYQLLDANNPFGSIAKLLRQS
                     AVDKATKIYNSKADDAEKIKSTKIVESSYSKTERNLILELGRIGNNVNQIAKAINTDIA
                     GTGTFDKVKLLHLLISIDQQLRELRPDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          3..496
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="8.17e-79"
     Typing          4280..5592
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 tggggtatta agtgatgacc gaattagaaa cacagttatt gaaaatcatc gaagaacaac
       61 agcagcaatt acagcaacaa caaactcagt tgcagcagca acagcagagt ttagacgtga
      121 tgcaaaagaa aattatccaa gccttatcag cacaatcaaa ggcaatggag agcgtacaga
      181 cagacttagt gcaaacacaa aagtcattat cgacaataca gacacaattt cacgaattgg
      241 aaaaaaatac agcgacctat accgagcaga tcaatggcaa agtcagggag atgagccaaa
      301 ttacaacgaa ttacgaggac gtactggaga aaatctcaac cacacaacgg tcagtctcgt
      361 cacaagtagc caaagctcag cagaatttgc tcgaaacgtc cgaactattg aagcgaatat
      421 cgtccaaatg aaggaacgga aaaaggaaat ggagcagccc caacctaagc gagatcgggg
      481 catgaatttt ggcatgtagg gtttatgctc gattccgact gttgctaatc aaaaatatgg
      541 aacaatgatt aaatattagg cgtgaaacgt gaagaatcaa taaaaaagcc cgacttagac
      601 aagttaggct tttttattga ttgtaaatta ttgaaataat taaactaatt ttgtatttat
      661 gtgcactaat gtgtgcacat aaaagcggag actttaattt ggtagggttc tttaagccca
      721 gtcatttcat cctaatcatt tggtcgagtg acaattggaa aagttgagtc tggacgttta
      781 aacattgcaa agaattttcc aatttccaat ggattgcctt tgactttact ttcgttagaa
      841 agcaggactt ttaccccttt tatttgacct gtaatcattt ttagatataa gggcttggtt
      901 acggttaatg ttgggcaatc cgatgccaaa tctttactgt catcatgacg cttaaactgc
      961 atgattgagt tttcaaccca taatgaaaaa ttctcttggg tatccgacaa aaccagattg
     1021 atacactggt tttcattttt taaattttcc acatctaaat ttgtcgccat ggcttcaaga
     1081 aatctttcag ttggcgtgtg aatcaataaa tcactcggat cagatgtatt ttgtaggggt
     1141 acattatttt gtagctcttg agcacccacc aaaaagaaat tacgccaagt tgaagcctca
     1201 gcagcatagc ccaattgacg atacgtattt gccaataagt cttttgcttg ttggttttga
     1261 gggttattca acacaatatg ttttaaaatc tcggcagccc atctatagtc agcttgagca
     1321 taggcatccc gcgcattttt taatgcatta ttctccccac ctgccaactc aatatatttt
     1381 tttgcaacag ctttaggcgg taatggatct aaattggaag gatgagcatc gaaccatccc
     1441 atatagtatt ggtatatcgc ttttacatta tgctttaagg ttccataata gccatgcgca
     1501 tatagctttt gatcaagtgc cgctggaagc tgaattttct cggcaatttc agcaccatta
     1561 aaaccagagt tcatatatct caccgtctga tcatgtgtga acttataaac atctcgctgg
     1621 gttttaataa aatcttgaat attgtcattg ccccagacag gccaatgatg ttgtgcaatc
     1681 aatacatcgg atgctttggc atgctgcatt gcttggtcta aatagcctac ccattttaag
     1741 gcatcacgga ctttggcacc gcggagggta taaagattat gcatggtatg cgaaagaatt
     1801 tctgcgccat tataaagttt cagtgatggg atagagaagg ttaactctgc tggcgcttct
     1861 gaacccggca cgttataaaa cacaaaatcc aaaccatcaa tggtcatttt ctgttctgct
     1921 tgggtgatca gttgtgtagg ctcaaggatt cccattttcc caacagcaac agccttacct
     1981 aaacctgtat ccaccaatcc ttcagcattc tttggtaaaa aggttccata catataggtc
     2041 gcacgacgta tcatggctga acctgccatc aaattttcac tggtcgcttc ttccataaag
     2101 ccttgtggcg caataattgg ggtttttcta gcgttaattt cttctgtaga aagaacgcct
     2161 aatgcaccac caaaatgatc gatatggcta tgggtaaaga taatggctga aattttttgc
     2221 tcacctaaat gctgcctagc aaatttaagt gctgcttcag ccgtttcttt agaggttaat
     2281 gtatccacaa taatccagcc agtatcaccc tgaataatgg tcatatttgc caaatcaaaa
     2341 ccacgaattt gccaaactcg atctgtcact ttaaatagac caacattatt gttgagcttt
     2401 gcttgtcgcc atagtgcagg attaacagta tttggtgctg gatcatttaa aaaattaaac
     2461 gcatcaaaat cccagatcac attgcctttt tcacttttaa tttgacctgt tggcttggca
     2521 ataaagcctt tttgcgcata attaaactct gtcatatcgt ctaaattata tttcgtttta
     2581 tacgcattat ttgattcaat gaccgctttt tcgacttgtc catttgctgc aaaaacagaa
     2641 aatggcaaaa taaacatcaa tgaaaccgtt aaaaatttaa ctttcattgt ctttccttct
     2701 tcaaaatatc cataaatgtt gatttccttg tctatactat ttttgatatg tgttgaatta
     2761 atagaagctt ttcaattcaa acattataat aacagccatc attttttatg atggaatcat
     2821 aaaataatat tttacaagaa tatacctgtt tttattaaaa ctacgcagtc cttattgggc
     2881 atgagttagt aggagagaaa aataaaaatt ttgttaaaac ctaaagaaaa acccctctga
     2941 gtcttgcaac ttagaggggt tttgtcttaa atttggtggt acgcagttat ttgatgttcg
     3001 agcatcaagt aacgaagatc ataaggtttg acgaccgtta tgatttcacc tttcggcttg
     3061 taccgctttt caaattctat caaattgata aaaaatggca agcattgtat cagtaaaaag
     3121 tctttcgtta cctgactctc gttcatccaa taagcaatac atagacagta ttcgagcctt
     3181 atcagcttct gtgcatcctt tttaaggaag atatgcggtg catgtggagg gaataagtgt
     3241 cactggtgca tcgtagcgat agtgaaagct gagaagcagg agctgaaccg ttacagggga
     3301 atcccacaaa agcggagcgt tgagagtagt tgtataggtt ggttaagttg catgttggag
     3361 aaatcctcgc agagataccg ccatacttcg acagggagcg cgtggggaga ggtgcagtac
     3421 ctttagtcct tgtctgccat gtatgtgaag catggtgcgg atacgaccga tgcgtggctc
     3481 cgtcagccta atcaggttaa atcggcaata cttaggggat gtgttatttt ttgaatgaca
     3541 gcccttaggg agagtattgc cgaaactctc tcaacgctca ccatctgcct gagcaaagcg
     3601 agggctgtgg tggtggtgag aaagagttga gtgctattgt tttacaagat ttatgccagt
     3661 tcttattagg gtttatatac tgtatcattt gattaaattt tagtataaat aattctttcc
     3721 ctttttaaat aagcaaggat acacagttac ccctatttca cttcgtttaa tagtgctaag
     3781 ctgtgacctt gatcaggggc ttcacccccg atacccccga aaaccggttt aaagaaaaat
     3841 caaagatttt cctctaaacc ggttagacca agaatgggat tgttcaagtg gaaatagatt
     3901 cagtaactaa acagaaagta gtacgggaac ggacgtttaa agtcagactt tcagaatcag
     3961 aatatcaatt gctcgatgcc aataatcctt ttggttcaat cgcaaagttg ctacgtcagt
     4021 cagcagtaga taaagcaaca aagatttata attccaaagc agatgatgct gaaaaaatta
     4081 aatcaacaaa gattgttgaa tcttcatata gcaagacaga gagaaattta atcctagaat
     4141 tgggtcgtat aggtaataac gtgaatcaga ttgccaaagc gattaatacg gatattgcag
     4201 ggacaggtac ttttgacaaa gtgaagttac ttcatttgtt gatttccata gatcaacaac
     4261 ttcgggagtt acgaccagat gatcgttgat ttttttagac atggttcagg attatctaaa
     4321 ggttgtctag attatttgct tggtgaagat agagagcgag aacatgcaca ggttttaagt
     4381 ggtgatgttg acttgaccgc tcaactgatt gatagcagtc cttttgccaa gaaatatacc
     4441 agtggttgtt tatcctttta cgagcatgct ttatctgacc aggataaaca gcagatcatg
     4501 caaaattttg aggaatgcct tttccctggt ctcgataaag atcagtacca aattttatgg
     4561 gtacagcacc aagataaggt gaatcaagat actggagaga cacggcttga gctgaatttt
     4621 gtgattccaa acgttgagct ttcgacaggc aaacggctac agccatttta tgcgccagtg
     4681 gatttagatc gagtggatct gtttaagcag attaccaatg cagaacatca actatatgat
     4741 cccgatgatc cgaacaatca gcagctttta atcaataaga agaacttacc caaggatgtg
     4801 aaggatttta aagatcagtt acaccaaagg atttaccaag ctgtagtcga tggcaaagtt
     4861 gccgatcggc aaaaattggt tgaatggctt gaatcgaatg aaatcaaagt cacccgacaa
     4921 acccaaaaat caatatcaat cgaaaatcca tacgagggcg caacacgtcc tatccgttta
     4981 agtggagagg tttatgagca aggctttagc gctactggcg aatatcgaca agaagtacaa
     5041 cagcgaattg caacgtaccg aggaacaact tctgagcggt atcgagcaaa cgtcacagat
     5101 tatcaaagac agcttgagca caagagccaa tatcacagcg accgctatcc agcagtcaga
     5161 cgagaaatta gcgcagaacc tacagaacag ctcacagcag atcgagaagc agctcgacca
     5221 gtaccaagcc ttgctacagt ccaaattgag ccgtttaaag caaatgaacg agcagataca
     5281 gacaaaagaa cagcaagttc agaacaaaag cctactgaaa cagcttacca ctttgaatat
     5341 ggcaatgatt ttgggagctg ctattttaat tattctcatt attgcgctcg gttttatggg
     5401 caaaagcaag tacaacgaga tacggacact caatatgacc atcaacgaga aacaaatgcg
     5461 agtggatcac ctgaaccgat cggggggcag tttgacgttc agcacatgca gacagcaaaa
     5521 cagcaaagcc caacgtctat gtattcagat caacaaggac gcaggggatt ggcagaacaa
     5581 cttcatggtg ccga
//
