LOCUS       NZ_CP133669             6078 bp    DNA     circular CON 13-SEP-2023
DEFINITION  Acinetobacter bereziniae strain UCO-553 plasmid p6kb_UCO-553,
            complete sequence.
ACCESSION   NZ_CP133669
VERSION     NZ_CP133669.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN34266403
            Assembly: GCF_031326185.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter bereziniae
  ORGANISM  Acinetobacter bereziniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 6078)
  AUTHORS   Opazo-Capurro,A.F., Xanthopoulou,K., Gonzalez-Munoz,P., Jerez,C.,
            Aguilera,F., Matus,M., Amsteins,L., Higgins,P.G. and
            Gonzalez-Rocha,G.
  TITLE     Characterisation of plasmids harbouring the tigecycline-resistance
            gene tet(Y) among carbapenem-resistant Acinetobacter bereziniae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6078)
  AUTHORS   Opazo-Capurro,A.F., Xanthopoulou,K., Gonzalez-Munoz,P., Jerez,C.,
            Aguilera,F., Matus,M., Amsteins,L., Higgins,P.G. and
            Gonzalez-Rocha,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2023) Microbiologia, Universidad de Concepcion,
            Barrio Universitario S/N, Concepcion, Bio-Bio 4070386, Chile
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/11/2023 16:11:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,329
            CDSs (total)                      :: 4,226
            Genes (coding)                    :: 4,160
            CDSs (with protein)               :: 4,160
            Genes (RNA)                       :: 103
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 25 of 66
            Pseudo Genes (incomplete)         :: 46 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 16 of 66
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP133669.1.
            Bacteria and source DNA available from Laboratorio de Investigacion
            en Agentes Antibacterianos (LIAA).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6078
                     /organism="Acinetobacter bereziniae"
                     /mol_type="genomic DNA"
                     /strain="UCO-553"
                     /isolation_source="tracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:106648"
                     /plasmid="p6kb_UCO-553"
                     /geo_loc_name="Chile: Santiago"
                     /lat_lon="33.26 S 70.38 W"
                     /collection_date="2021-02-16"
                     /collected_by="Hospital de Urgencia Asistencia Pblica"
     gene            join(5562..6078,1..1298)
                     /locus_tag="RG306_RS21605"
                     /old_locus_tag="RG306_21605"
     CDS             join(5562..6078,1..1298)
                     /locus_tag="RG306_RS21605"
                     /old_locus_tag="RG306_21605"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_000634474.1"
                     /translation="MIVDFFRHGSGLSKGCLDYLLGEDREREHAQVLNGDVELTAQLID
                     SSPFAKKYTSGCLSFYEHDLSDQDKQKIMQNFEECLFPGLDKDQYQILWVQHQDKINQD
                     TGETRLELNFVIPNVELSTGKRLQPFYAPVDLDRVDLFKQITNTEHSLYDPDDPEHRQL
                     FLNKKNLPKDIKDFKAQLHQRVYRAVANGDVADRQELVQWLESNQINVTRQVKNSISIE
                     NPYEGAKRPIRLEGEIYEQGFRATGEYRQEVQQRIEEYRGTTSERYRTNVTDYQRQLEH
                     KSQYHCDRYPTVGRENSPEHSKQRPDGREAVKPVSNLAAIEIEPFNAIKRANTEPRTTS
                     PESSGTEKTYHFEYGTDFSSSYFAYSNFLAWSRHQKQVQRDADAKYRDQREANEGRPFE
                     PIGRQFDDQHMQATRQQSTAPMYSDQQERRRMAERLHDSNGVLNDDRIRNTIIENHRRT
                     TATINATTSAIAEATASFRHNANQNYPSLVSTIKDNQERSAKLTASTEIISDNAEAISR
                     ARTKYSKLYRADQWQSPRDEPYHSQQRRNSGENLNHTAFCLVRSSQSAAEFARNIRDIE
                     KNLVQMNERKQEIEKPRPKQDRGMDFGM"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1407..1892
                     /locus_tag="RG306_RS21610"
                     /old_locus_tag="RG306_21610"
     CDS             1407..1892
                     /locus_tag="RG306_RS21610"
                     /old_locus_tag="RG306_21610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005200081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_051064109.1"
                     /translation="MNNNCVVVLTARGKTRILKEGGSQSWRLNAHNAGKCDYLVCVQNR
                     KQSWGEPEADHHTAFLVGKISSISQSQEEDADGRWIINISEYADISIPNMWDGNRNPVS
                     YSSLEEMNIDLNELKFTKLNEDNSSKTLDKISIPEAKRLLANYFDVPQENISITINM"
                     /name="hypothetical protein"
     gene            2025..2693
                     /locus_tag="RG306_RS21615"
                     /old_locus_tag="RG306_21615"
     CDS             2025..2693
                     /locus_tag="RG306_RS21615"
                     /old_locus_tag="RG306_21615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="WP_000423610.1"
                     /translation="MELSNDAVLKIEQALSIPRLSKYENFYKDKGEPYEKSDVLMLYER
                     NLIISNKFFYLLNYFEVVLRNAVVQAIEISFRCNETNSWHENEAFIRSLSRRGRYSPKS
                     MFDSAKEKFPDSPSKMIPELKFVFWQKMLMANYEERIWQGCFNSIFPNATVENRQIFYD
                     WTDQLRELRNRIAHHEPIIFNRNLENDLEVLTKFIYCRCINTYDFIEQSALDLKEYLDF
                     "
                     /name="Abi family protein"
     gene            complement(2852..3385)
                     /gene="aadB"
                     /locus_tag="RG306_RS21620"
                     /old_locus_tag="RG306_21620"
     CDS             complement(2852..3385)
                     /gene="aadB"
                     /locus_tag="RG306_RS21620"
                     /old_locus_tag="RG306_21620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase
                     ANT(2'')-Ia"
                     /protein_id="WP_010883707.1"
                     /translation="MDTTQVALIHQILAAADERNLPLWIGGGWAIDARLGRVTRKHDDI
                     DLTFPGERRGELEAIVEMLGGRVTEELDYGFLAEIGDELLDCEPAWWADEAYEIAEAPQ
                     GSCPEAAEGVIAGRPVRCNSWEAIIWDYFYYADEVPPVDWPTKHIESYRFACTSLGAEK
                     VEVLRAAFRSRYAA"
                     /name="aadB"
     gene            complement(3476..4162)
                     /locus_tag="RG306_RS21625"
                     /old_locus_tag="RG306_21625"
     CDS             complement(3476..4162)
                     /locus_tag="RG306_RS21625"
                     /old_locus_tag="RG306_21625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004986708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="WP_002011386.1"
                     /translation="MSKLPRFHNKHIEKRKYSNSLLAKSISSKVRFSNKTFFNVNLRGS
                     RLRSCSFKNIKIVLSDFSGVILNKNRFTNVHFKKCVFYAAIFRDCRFKNCTFEKCIFIN
                     TNTESLNHSILDCSKKYNFYEMKIPDDLKDQLTEYRNIPILQKNRLLHLKGGRINTATL
                     FILLSKISKYKLLNGLKKIYIEEQSKVKILSTFKLLEEIKNLVDTNTAFKQNNGALPHP
                     IERLDS"
                     /name="pentapeptide repeat-containing protein"
     gene            5165..5572
                     /locus_tag="RG306_RS21630"
                     /old_locus_tag="RG306_21630"
     CDS             5165..5572
                     /locus_tag="RG306_RS21630"
                     /old_locus_tag="RG306_21630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000016535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_281104429.1"
                     /translation="MSGQEQENKQSKAVRERTFKVRLSESEYQLLDSSNPYGSIAKLLR
                     QAAVEKATKIHNSKAEDDQKIKATKVVESSYSKTERALILELGRIGNNVNQIAKAVNTD
                     IAGVGTFDKVKLLHLLISIDQQLRELRPDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     ARG             complement(2856..3385)
                     /name="ant(2'')-Ia"
                     /gene="ant(2'')-Ia"
                     /gene_name="aminoglycoside nucleotidyltransferase
                     ANT(2'')-Ia"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="98.31"
                     /coverage="100.0"
     ARG             complement(2853..3397)
                     /name="ANT(2'')-Ia"
                     /gene="ANT(2'')-Ia"
                     /gene_name="ANT(2'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="97.74"
                     /coverage="102.26"
     Typing          4..1295
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          5563..6077
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="1.17e-108"
ORIGIN
        1 atattaagga ttttaaagcg cagttacatc aacgtgttta ccgagcggtt gccaatggtg
       61 atgttgccga tcggcaagaa ttggtgcaat ggcttgaatc gaatcaaatc aatgtcactc
      121 gacaagtaaa aaactccata tccattgaaa acccatatga gggcgcaaaa cgtcccatcc
      181 gtttagaggg cgagatttat gagcaaggct ttagagctac tggcgaatat agacaagaag
      241 tacaacaacg aattgaagaa tacagaggaa caacttctga gcggtatcga acaaacgtca
      301 cagactatca gagacagctt gaacataaga gccaatatca ctgcgaccgc tatccaacag
      361 tcggacgaga aaatagccca gagcattcaa aacaacgccc agacggtcga gaagcagtta
      421 agcctgtatc aaaccttgct gcaatcgaaa ttgagccatt taacgcaatt aaacgagcaa
      481 atacagagcc aagaacaaca agcccagaat caagcggtac agaaaaaact taccactttg
      541 aatatgggac tgatttcagt agcagttatt ttgcttatag caattttctc gcttggtctc
      601 gtcaccaaaa acaagtacag cgagatgcag acgctaaata ccgtgatcaa cgagaagcaa
      661 atgagggtcg accatttgaa ccgatcggga ggcagtttga cgatcagcac atgcaagcga
      721 ccagacagca aagcacagcg cctatgtatt cagatcaaca agaacgcagg cgaatggcag
      781 aacggctaca tgattccaat ggggtattaa acgatgaccg aattagaaac acaattattg
      841 agaatcatcg aagaacaaca gcaacaatta atgcaacaac aagtgcaatt gcagaagcaa
      901 cagcaagctt tagacacaat gcaaaccaaa attatccaag ccttgtcagc acaatcaaag
      961 acaatcaaga gcgatcagca aagcttacag caagcacaga aatcattagc gacaatgcag
     1021 aagcaatttc acgagctaga acgaagtaca gcaagctata ccgagcagat caatggcaaa
     1081 gtccgagaga tgagccttat cacagccaac aacgaagaaa ttctggagag aatctcaacc
     1141 acacagcatt ctgtctcgtc agaagtagcc aaagcgcagc agaatttgct cgaaacatcc
     1201 gagatattga aaaaaatctc gtccagatga atgagcgtaa acaagagata gaaaaacctc
     1261 gacctaagca agatcgaggt atggattttg ggatgtaatt attttctggt atgttggtgt
     1321 ttatttctta gtattagctt gtactaatgt ttaaatttag ctatattgaa tgtagtatag
     1381 gcttgtttat atattttggt ggtgttatga ataataactg tgtagtagta ctaactgctc
     1441 gtggaaagac tagaatttta aaagaaggtg gtagtcagtc ttggagacta aatgctcata
     1501 atgcaggaaa gtgcgattat ctagtttgtg tgcaaaatag gaaacaatct tggggagaac
     1561 cagaggctga tcatcacact gcatttttag ttggcaaaat atctagtatc agccaatctc
     1621 aagaagaaga tgcagatggt agatggataa taaatatctc cgaatatgct gatatatcta
     1681 ttcccaatat gtgggatggt aatcggaatc cagtttctta tagttcatta gaagagatga
     1741 atatcgattt gaatgagctg aaatttacca aattaaatga agataattct tctaaaacac
     1801 tggataaaat atctattcct gaagctaaga gactattagc taattatttt gatgtccctc
     1861 aagaaaatat ttcaattaca atcaatatgt aagcttttaa ttgaccaaaa aaagaaaaaa
     1921 gcctttcacg ttaatgaaag gcttttttga atcttagcgc ttgggaggga agatcttaga
     1981 tccgcagtct ctcaaactta caactatata ttagggtata tacggtggaa ttgtcaaatg
     2041 atgcagtgct taagatagag caggctttat ctataccacg cttatctaag tatgaaaatt
     2101 tctataaaga taaaggtgaa ccatatgaaa aatctgatgt attgatgctt tatgaacgta
     2161 atttaataat ttccaataag tttttttact tattaaatta ttttgaagtt gttctgagaa
     2221 atgctgttgt tcaggcaata gaaatatctt ttaggtgtaa tgaaacaaat agttggcatg
     2281 aaaatgaagc ttttattcga agccttagcc gtagagggag gtatagccct aaaagtatgt
     2341 ttgatagtgc taaagagaag ttcccagatt cgcctagtaa aatgatccct gagcttaaat
     2401 ttgtattctg gcaaaagatg cttatggcta attatgaaga acgtatttgg cagggatgtt
     2461 ttaattctat ttttcctaat gcaacagttg aaaacaggca aattttttac gattggacag
     2521 atcaattacg tgaattaaga aacaggattg ctcatcatga accgattatt tttaaccgta
     2581 atctagaaaa tgatttagag gttttaacaa aatttattta ctgtcgttgt attaatacat
     2641 acgattttat cgagcaatct gcattagatc taaaagaata tttagatttt taagataaga
     2701 atttcacccc aaacaatcga aaggtcagac tattttgcaa atgttgaatt aacaaggtta
     2761 gtagtaaagc agtaaatccg ataacggtag cagtgtttcc taacacctga gttaagccgc
     2821 gccgcaaagc ggcgtcggct tggacgaatt gttaggccgc atatcgcgac ctgaaagcgg
     2881 cacgcaagac ctcaaccttt tccgccccga gtgaggtgca tgcgaacctg taggactcta
     2941 tgtgctttgt aggccagtcc actggtggta cttcatcggc atagtaaaag taatcccaga
     3001 tgatcgcctc ccagctgtta caacggactg gccgcccggc gatgacaccc tcagccgcct
     3061 ctgggcacga gccctgcgga gcctccgcga tttcatacgc ttcgtctgcc caccaagcag
     3121 gttcgcagtc aagtaactca tccccgatct ccgctaagaa tccatagtcc aactcctccg
     3181 tgacgcgccc gccgagcatt tcaactattg cctcgagctc gccgcgcctc tcgccgggaa
     3241 aagtcagatc aatatcatcg tgcttgcgtg ttacacgccc tagccgtgca tcgatcgccc
     3301 agcccccacc gatccagagc ggcagatttc gctcatctgc cgcagctaga atttggtgta
     3361 tcaatgcgac ctgcgttgtg tccatgcggc ctaacatagt aagccctccc agaatgtcag
     3421 taattagact attaattgaa ttgttacatc caatagtgag ctcccagtga ttgtcttaac
     3481 tatccaacct ttcgattgga tggggcagag caccattatt ctgtttgaat gcggtgttag
     3541 tgtcaactag gttctttatt tcttcaagga gtttgaaagt gctaagtatt tttactttac
     3601 tttgttcctc aatataaatt tttttaagac catttagcag cttgtatttg gatattttgg
     3661 ataggagaat gaataaggtt gcggtattga ttcttccccc ttttagatga agtaaacggt
     3721 ttttttgcag tattggaata tttctatatt ctgttagttg atcttttaag tcatcaggaa
     3781 ttttcatttc gtaaaaatta tattttttag aacagtctaa tatcgagtga tttaggcttt
     3841 ctgtatttgt attaataaaa atgcattttt caaatgtgca atttttaaag cgacaatctc
     3901 taaaaatagc agcataaaat acgcactttt tgaagtgaac attcgtaaac ctattcttat
     3961 ttaagattac tcctgaaaaa tcagataaaa caatttttat atttttgaat gaacatgatc
     4021 gtaggcggga tcctctcaaa ttaacattaa aaaatgtttt attagagaaa cgtactttag
     4081 aagaaataga tttggctagg aggctatttg aatacttacg tttttcgatg tgcttgttat
     4141 gaaatctagg aagttttgac attaatagtc caaagaatag gagtttcagc ttaattctta
     4201 ggtgtaatca aaaaaaataa tggacaggtc tcttgcgaaa tctgtccatt agttttaaat
     4261 tcgatgggta cagttatttg atgcttgcaa catcaattaa cgaaagatcg gcagagcttg
     4321 caactcatac tgatattcac cttaaaggct agtaccacta tctattttaa agaattagaa
     4381 cagtttgaca agcctcaatt tgaagtcctt cgttaattca gcagtttaaa tcatcaaata
     4441 agcacctaac ggtcgagctt tatctacttc tgtgcgcctt ttttaaggaa gatatgcgtt
     4501 gcatgtggag ggaataagtg tcactggtgc atcgtagcga tagtcgcaag gctaaaccgc
     4561 ttacagggga tctcacatag gcggagcgtt ggaagtagtt gtataggttg gttaagttgc
     4621 atgttggagt aatcctcgca gagataccac catacttcga cagggagcgc gtgggagaag
     4681 gtgcaaaacc tttagtcctt gtctgcatgg tcagcaaaac catgtgcgga tacgaccgat
     4741 gcgtggctcc gtcagcccaa ttacaggtta atcggcaata cttaagagga tatttgattt
     4801 ttttaaatca aagcctctta gggaaagtat tgccgaaact ctctcaacgt tcaccatcag
     4861 cccgagcaaa gcgagggatg tggggacggt gagaaagagt tgagcgcatt tgtttttaca
     4921 agattcatgc caatacctac tagagtttat ataccgtgtc atttgattag tttttattga
     4981 atttcactcg tccattttta aataagcaag gatacacagc tacccctatt taacttcgtt
     5041 aaacagtgat agtctgtgac cttgatcagg ggcttcaccc ccgatacccc cgaaaagctt
     5101 taaacagaaa aatcaaagat tttccaattt aaagcttaca aaagccaaag cagggtagat
     5161 tgaaatgtca ggacaggaac aagaaaacaa acagagcaaa gcagttcgag aacgaacctt
     5221 taaagtcaga ctttcagaat cagaatatca attgctcgat tccagtaatc cttatggttc
     5281 gattgcaaag ttgcttagac aagcagcagt agagaaagcg actaaaattc ataattccaa
     5341 agcagaagat gaccaaaaaa ttaaagcaac aaaggttgtt gaatcttcgt atagcaagac
     5401 agagcgagca ttaattttag agcttggtcg gattggcaac aatgtgaatc agattgctaa
     5461 agcagtgaat accgatattg caggggtagg tacttttgac aaagtgaagt tacttcattt
     5521 gttgatttcc atagatcagc agcttcggga gttacgtcca gatgatcgtt gatttcttta
     5581 gacatggttc aggtttatca aaaggctgtc tcgattattt gttaggtgaa gatcgagagc
     5641 gtgaacatgc tcaagtgctc aatggtgatg tagaactgac agcacaactc attgatagca
     5701 gtccttttgc caaaaaatat accagtggtt gtttgtcctt ttatgagcat gatttatctg
     5761 accaggataa acagaaaatc atgcaaaatt ttgaggaatg tttattcccg ggactagata
     5821 aagaccaata tcaaatttta tgggtacagc accaagacaa aattaatcag gacacaggtg
     5881 agacaaggct tgagcttaat tttgtgattc ctaatgttga gctttcaaca ggcaaacgcc
     5941 tacagccatt ctatgcgcca gtcgatttag atcgagtcga tctatttaag cagatcacta
     6001 ataccgaaca ttcactgtat gaccccgatg atcctgagca tcgacagcta tttttaaata
     6061 aaaagaacct acccaaag
//
