LOCUS       NZ_CP133665             2366 bp    DNA     circular CON 13-SEP-2023
DEFINITION  Staphylococcus epidermidis strain AR4072 plasmid p-unnamed1,
            complete sequence.
ACCESSION   NZ_CP133665
VERSION     NZ_CP133665.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37262287
            Assembly: GCF_031326165.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus epidermidis
  ORGANISM  Staphylococcus epidermidis
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Chen,Y.
  TITLE     A mosaic plasmid carrying a Tn552-related fosD resistance gene in
            Staphylococcus epidermidis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Chen,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2023) Infectious Diseases, Zhejiang University, 3
            East Qingchun Road, Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. 0.4.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/11/2023 15:53:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,741
            CDSs (total)                      :: 2,660
            Genes (coding)                    :: 2,592
            CDSs (with protein)               :: 2,592
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 40 of 68
            Pseudo Genes (incomplete)         :: 37 of 68
            Pseudo Genes (internal stop)      :: 22 of 68
            Pseudo Genes (multiple problems)  :: 23 of 68
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP133665.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Staphylococcus epidermidis"
                     /mol_type="genomic DNA"
                     /strain="AR4072"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1282"
                     /plasmid="p-unnamed1"
                     /geo_loc_name="China:hangzhou"
                     /lat_lon="30.3 N 120.2 E"
                     /collected_by="feng zhao"
     gene            complement(390..866)
                     /locus_tag="RGC89_RS13700"
                     /old_locus_tag="RGC89_13700"
     CDS             complement(390..866)
                     /locus_tag="RGC89_RS13700"
                     /old_locus_tag="RGC89_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664727.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_000664727.1"
                     /translation="MKERYGTVYKGSQRLIDEESGEVIEVDKLYRKQTSGNFVKAYIVQ
                     LISMLDMIGGKKLKIVNYILDNVHLSNNTMIATTREIAKATGTSLQTVITTLKILEEGN
                     IIKRKTGVLMLNPELLMRGDDQKQKYLLLEFGNFEQEANEKQENALSDYYSFKD"
                     /name="replication/maintenance protein RepL"
     gene            complement(1314..2048)
                     /gene="erm(C)"
                     /locus_tag="RGC89_RS13705"
                     /old_locus_tag="RGC89_13705"
     ARG             complement(1318..2048)
                     /name="erm(C)"
                     /gene="erm(C)"
                     /gene_name="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(C)"
                     /tool="amrfinderplus"
                     /drug_class="LINCOSAMIDE/MACROLIDE/STREPTOGRAMIN"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(1315..2048)
                     /name="ErmC"
                     /gene="ErmC"
                     /gene_name="Erm 23S ribosomal RNA methyltransferase"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic; lincosamide antibiotic;
                     streptogramin antibiotic; streptogramin A antibiotic;
                     streptogramin B antibiotic"
                     /identity="99.59"
                     /coverage="100.0"
     Typing          complement(381..866)
                     /name="rep_cluster_1947"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.591"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ctattggttg gaatggcgtg tgtgttagcc aaagctttgg cgagttggtt gggggtttca
       61 tgggattaat cccatgaaag taccaactca acaacacact aacgcctgtt ggttccaacc
      121 aataggaaat tggaataagc aattagtata atgagagtat aatgttggta taacgttagt
      181 ataatgatgc tttttttcat tatatttttt atgtacttta aacctgcacg cttatgtgaa
      241 ttagaaaaag cttaatcgca tttcatagat tgacctccca ataactacgt ggtgttattg
      301 ggaggtcaat ctatttcatt tgcgttaagc ttttgatcta cattaagcgt gcaggttttt
      361 tgtctatttg tagacgattt tatgttatac tagtccttga aagaataata atcagataat
      421 gcattttctt gtttttcatt tgcctcttgc tcaaagttcc caaattcgag taagaggtat
      481 ttttgttttt ggtcgtcgcc tctcattagt agttcagggt ttaacattaa tactccagtt
      541 tttcttttta taatatttcc ttcttctaag attttaagtg ttgttattac tgtttgtaga
      601 cttgttcctg tagcttttgc tatttctctt gttgtagcta tcattgtatt gttacttaag
      661 tggacattat ctaggatata gttaacgatt ttaagttttt ttccgccaat catatctaac
      721 atacttatta attgcactat atatgccttt acgaagttac cagacgtttg tttacggtat
      781 aacttgtcta cctctatgac ttctccactt tcttcgtcta tgagcctctg agagccttta
      841 tagactgttc catatctttc tttcatcttt ttctcactcc ttattttaaa ctattctaac
      901 tatatcataa ctgttctaaa aaaaaaagaa catttgttaa aagaaattag aacaaaatga
      961 gtgaaaaatt agaacaaaca aattccttat aaaccttatc atctcaacct atattaagat
     1021 tttacctagt tgaatcttct tttctatata aagcgtcgga gcatatcagg gggttatcta
     1081 acgtaaatgc tacccttcgg ctcgctttcg ctcggcattg acgtcagata ctgcaccccc
     1141 tgaaccccca tgctccaaca gcaaaaagga aactttttgc tgcttttccg acgcttattc
     1201 gcttcgctca tatttatata gaaaagaagt gaatgcgcaa aagacataat cgattcacaa
     1261 aaaataggta cacgaaaaac aagttaaggg atgcagttta tgcatccctt aacttactta
     1321 ttaaataatt tatagctatt gaaaagagat aagaattgtt caaagctaat attgtttaaa
     1381 tcgtcaattc ctgcatgttt taaggaattg ttaaattgat tttttgtaaa tattttcttg
     1441 tattctttgt taacccattt cataacgaaa taattatact tctgtttatc tttgtgtgat
     1501 attcttgatt tttttctatt taatctgata agtgagctat tcactttagg tttaggatga
     1561 aaatattctc ttggaaccat acttaatata gaaatatcaa cttctgccat taaaaataat
     1621 gccaatgagc gttttgtatt taataatctt ttagcaaacc cgtattccac gattaaataa
     1681 atctcatcag ctatactatc aaaaacaatt ttgcgtatta tatccgtact tatgttataa
     1741 ggtatattac caaatatttt ataggattgg tttttaggaa atttaaactg caatatatcc
     1801 ttgtttaaaa cttggaaatt atcgtgatca acaagtttat tttctgtagt tttgcataat
     1861 ttatggtcta tttcaatggc agttacgaaa ttacacctct gtactaattc aagggtaaaa
     1921 tgcccttttc ctgagccgat ttcaaagata ttatcatgtt catttaatct tatatttgtc
     1981 attattttat ctatattatg ttttgaagta ataaagtttt gactgtgttt tatatttttc
     2041 tcgttcatta taaccctctt tattttttcc tccttataaa attagtataa ttatagcacg
     2101 agctctgata aatatgaaca tgatgagtga tcgttaaatt tatactgcaa tctgatgcga
     2161 ttattgaata aaagatatga gagatttatc tagtttcttt ttttacaaga aaaaagaaag
     2221 ttcttaaagg ttttatactt ttggtcgtag agcacacggt ttaacgactt aattacgaag
     2281 taaataagtc tagtgtgtta gactttaatg tttttttaag gcattagtgc atttaagcgt
     2341 cagagcatgg ctttatgccg agaaaa
//
